Biotechnologia最新文献

{"title":"Search continues: Exploring immunoinformatics platforms for designing an effective multiepitope malaria vaccine candidate.","authors":"Charles Osuji, Godwin Etuk-Udo","doi":"10.5114/bta/204528","DOIUrl":"https://doi.org/10.5114/bta/204528","url":null,"abstract":"<p><strong>Background: </strong>The prevailing public health threat posed by malaria, especially in developing countries, remains a serious concern despite the availability of preventive and control measures. While vaccination offers a powerful means of combating malaria, it has not been fully exploited due to previous unsuccessful attempts before the launch of the RTS,S vaccine. A major challenge in malaria vaccine development continues to be the identification of effective targets capable of eliciting robust immunity, given the complexity of the parasites' life cycle. Leveraging on the breakthrough of the newly approved malaria vaccine, efforts to develop more effective prophylactic solutions continue with renewed determination.</p><p><strong>Materials and methods: </strong>In this study, a standard structural bioinformatics pipeline was employed to design a multiepitope subunit vaccine against <i>Plasmodium</i>, particularly <i>P. falciparum</i>. Thirty subunit epitopes were mined from selected variant surface antigens of <i>P. falciparum</i> proteins expressed at different stages of its life cycle, based on their vaccine-likeness. These epitopes were conjugated with suitable adjuvants and linkers into a vaccine construct, which was then subjected to stringent downstream analyses.</p><p><strong>Results: </strong>Out of an initial pool of 133 epitopes, 30 vaccine-fit epitopes were selected, resulting in a final vaccine construct comprising 570 amino acid residues. This included 12 linear B-cells, 11 cytotoxic T-lymphocytes, and 7 helper T-lymphocyte epitopes, all with favorable predicted structural, antigenic, and physicochemical properties. The construct also demonstrated strong global population coverage (95.04%), robust molecular binding, and simulated immune responses.</p><p><strong>Conclusions: </strong>With the evolving \"Omics\" technologies through reverse vaccinology, discovering and designing promising vaccine candidates becomes easier without many challenging experimental rigors. This study highlights the potential of immunoinformatics-aided approaches in accelerating effective malaria vaccine development.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 2","pages":"151-168"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144700883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethanol extract from <i>Argyreia acuta</i> Lour. leaves exhibit analgesic, antipyretic, and anti-inflammatory effects in mouse models.","authors":"Tran Thi Phuong Nhung, Le Pham Tan Quoc, Dang Thi Kim Thy","doi":"10.5114/bta/204527","DOIUrl":"https://doi.org/10.5114/bta/204527","url":null,"abstract":"<p><strong>Background: </strong><i>Argyreia acuta</i> has traditionally been used for its analgesic, antipyretic, and anti-inflammatory properties; however, scientific validation of these effects remains limited. This study aimed to evaluate the pharmacological potential of ethanol extract from <i>A. acuta</i> leaves (AAEE) in murine models of pain, fever, and inflammation.</p><p><strong>Materials and methods: </strong>The pharmacological properties of <i>A. acuta</i> leaf extract were assessed. Analgesic activity was evaluated using a hot plate and tail-flick assays, while antipyretic effects were tested via a yeast-induced pyrexia model. The anti-inflammatory potential was investigated through carrageenan-induced paw edema and by quantifying pro-inflammatory mediators, including TNF-α, IL-1β, IL-6, COX-2, and PGE₂. Histopathological analysis of paw tissues was performed to confirm inflammatory changes.</p><p><strong>Results: </strong>AAEE exhibited significant, dose-dependent analgesic effects, as indicated by prolonged latency times and increased pain inhibition (<i>p</i> < 0.05), with the 200 mg/kg dose showing the greatest efficacy. In the antipyretic model, AAEE at 200 mg/kg reduced rectal temperature to 36.93°C, corresponding to an inhibition rate of 82.61% (<i>p</i> < 0.05). The extract significantly reduced paw edema (41.39% inhibition at 200 mg/kg) and markedly lowered levels of TNF-α, IL-1β, IL-6, COX-2, and PGE₂ (<i>p</i> < 0.05). The histological analysis supported these findings, revealing decreased edema and inflammatory cell infiltration in treated groups.</p><p><strong>Conclusions: </strong>These findings provide scientific support for the traditional use of <i>A. acuta</i>, demonstrating its significant analgesic, antipyretic, and anti-inflammatory activities. AAEE may represent a promising natural therapeutic agent for treating pain, fever, and inflammation.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 2","pages":"169-182"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144700878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of bioeconomy within regional policy frameworks: a case study of the Wielkopolska Voivodeship.","authors":"Ewa Woźniak-Gientka","doi":"10.5114/bta/205149","DOIUrl":"https://doi.org/10.5114/bta/205149","url":null,"abstract":"<p><strong>Background: </strong>Recently, many countries and regions have started developing targeted bioeconomy strategies and plans. The concept of the bioeconomy has evolved since the implementation of the first European Union bioeconomy strategy in 2012. Current trends include, among others, the circular economy, biotechnology, and sustainable development.</p><p><strong>Materials and methods: </strong>This article examines the role of the bioeconomy in the strategic documents of the Wielkopolska Voivodeship, using a case study approach along with analytical and critical methods. Additionally, it explores local government initiatives supporting bioeconomy development, identified through interviews.</p><p><strong>Results: </strong>In Wielkopolska, there is currently no formal plan to develop a dedicated bioeconomy strategy. However, numerous documents and plans related to the bioeconomy have been developed since 2020, aligning regional policies with the objectives of European Union policy. The local government of Wielkopolska is actively engaged in food waste reduction through education, investment, and support for NGOs and entrepreneurs. There is strong institutional support for bioeconomy-related innovation, including dedicated strategies, funding, competitions, and stakeholder cooperation platforms.</p><p><strong>Conclusions: </strong>A regional bioeconomy strategy is essential for leveraging local resources, addressing region-specific challenges, and aligning with EU policy frameworks. While Wielkopolska currently lacks a formal strategy, elements of the bioeconomy are included in existing plans. Developing regionally tailored strategies, fostering public awareness, enhancing education, and encouraging cross-sector collaboration is key to building a sustainable, innovation-driven bioeconomy.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 2","pages":"195-208"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144700880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of proteomics and bioinformatics in traumatic brain injury biomarker discovery.","authors":"Mohamed M Mohamed, El-Sayed A El-Absawy, Hala M Ahmed, Mohamed E Hasan","doi":"10.5114/bta/202470","DOIUrl":"https://doi.org/10.5114/bta/202470","url":null,"abstract":"<p><strong>Background: </strong>Traumatic brain injury (TBI) is a significant medical crisis with no FDA-approved therapies to improve functional outcomes. Key biomarkers, such as glial fibrillary acidic protein (GFAP), S-100 calcium-binding protein B (S-100B), and ubiquitin C-terminal hydrolase L1 (UCH-L1), are crucial for understanding TBI pathology.</p><p><strong>Materials and methods: </strong>This study integrates proteomic and bioinformatic approaches to explore established TBI biomarkers' structural and functional complexities: GFAP, S-100B, and UCH-L1.</p><p><strong>Results: </strong>Our comprehensive secondary structure and solvent accessibility assessment, conducted with PredictProtein, confirmed the predominance of alpha-helices in GFAP and S-100B, while UCH-L1 displayed a balanced mix of helices (65.00, 67.39, and 40.81%), beta strands (6.20, 0, and 17.94%), and coils (40.81, 17.94, and 41.26%). AlphaFold and I-TASSER were identified as the best servers for full-length tertiary structure prediction for the three target proteins, based on root-mean-square deviation (RMSD), TM-score, and C-score assessments. Protein motif database scans predicted four, eight, and one protein-binding motifs and two, three, and one post-translational modifications for GFAP, S-100B, and UCH-L1, respectively.</p><p><strong>Conclusions: </strong>GFAP's role in axonal transport and synaptic plasticity was emphasized through motifs such as Filament and DUF1664. S-100B's association with neuroinflammation and oxidative stress post-TBI was supported by the S-100/ICaBP-type calcium-binding domain. UCH-L1's dualistic impact on TBI was further clarified by the Peptidase_C12 motif. This approach deepens our comprehension of these biomarkers and paves the way for targeted diagnostics in TBI.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 2","pages":"123-150"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144700881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implications of mtDNA in human health and diseases.","authors":"Smruthi Seethashankar, Shruti Hariharan, Venkatachalam Deepa Parvathi","doi":"10.5114/bta/204532","DOIUrl":"https://doi.org/10.5114/bta/204532","url":null,"abstract":"<p><p>The maternally inherited autonomous organelles, mitochondria, are responsible for a myriad of functions within the cell. They may contain more than one copy of DNA and can themselves be present in multiple numbers within a cell. The integrity of the mitochondrial genome is affected by variations in DNA copy number or the presence of mutations. Compromising this integrity has been documented to result in disorders affecting various systems. Focusing on such trends could enhance knowledge essential for developing strategies to manage these disorders. Irregular patterns of mitochondrial DNA (mtDNA) copy number (CN) variation have been identified in various cancers. Reduced mtDNA CN has been associated with neurodegenerative disorders, cardiovascular diseases, and kidney disorders. Mutations in the mitochondrial respiratory chain complex have been linked to cardiomyopathy. High rates of mtDNA deletions have been found in aging patients and subjects with Parkinson's disease. While sperm function appears to deteriorate with increased mtDNA CN, oogenesis involves a significant increase to enable the oocyte to achieve fertilization and further development. Prospective therapies to treat mitochondrial diseases may include approaches that aim to reduce the levels of mutant mtDNA below the disease-causing threshold, such as targeted removal of defective mitochondria. Mutations in mitochondrial DNA contribute to various diseases; some single substitutions appear to disrupt the normalcy of more than one organ, underscoring the importance of mitochondrial genome integrity. The presence of mutations and copy number variations may serve as diagnostic markers and also provide insight into prognosis.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 2","pages":"209-222"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144700879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> screening of angiotensin converting enzyme and hydroxymethylglutaryl-coenzyme A reductase inhibitory activities in lactic acid bacteria and yeasts isolated from fermented sorghum gruels.","authors":"Praise Temilade Ozabor, Johnson Olaleye Oladele, Ilesanmi Festus Fadahunsi","doi":"10.5114/bta/205474","DOIUrl":"https://doi.org/10.5114/bta/205474","url":null,"abstract":"<p><strong>Background: </strong>3-hydroxyl-3-methylglutaryl-coenzyme A (HMG-CoA) reductase and angiotensin converting enzyme (ACE) are implicated in the pathogenesis of hyperlipidemia and hypertension, which are oxidative-stress linked conditions of public health importance. The adverse effects associated with standard clinical drugs used to inhibit these enzymes have prompted the search for alternative sources. This study was designed to investigate the <i>in vitro</i> inhibitory activities of lactic acid bacteria (LAB) and yeasts isolated from fermented sorghum gruels.</p><p><strong>Materials and methods: </strong>LAB and yeast isolates were obtained and characterized using standard methods. The HMG-CoA reductase and ACE inhibitory activities of the microbial isolates were evaluated using established protocols.</p><p><strong>Results: </strong>Screening of LAB for HMG-CoA reductase and ACE inhibitory activities revealed that at concentrations (mg/ml) of 6, 12, 24, and 48, <i>Lactobacillus pentosus</i> WSL5 exhibited the highest %HMG-CoA reductase inhibition of 3.21, 6.42, 9.17, and 12.84, with corresponding ACE inhibitory activities of 6.38, 13.17, 18.13, and 23.47, respectively. At concentrations (mg/ml) of 1, 2, 4, and 8, the yeast isolates <i>Trichomonascus ciferri</i> RSY53 demonstrated %HMG-CoA reductase inhibition of 7.71, 11.47, 14.68, and 16.97, with corresponding ACE inhibitory activities of 11.83, 20.91, 34.73, and 48.28, respectively. Furthermore, <i>L. pentosus</i> WSL5 recorded the lowest HMG-CoA reductase half-maximal inhibitory concentration (IC₅₀) of 219.72 µg/ml and ACE IC₅₀ of 116.22 µg/ml, while <i>T. ciferri</i> RSY53 had even lower IC₅₀ values of 29.55 µg/ml for HMG-CoA reductase and 7.03 µg/ml for ACE inhibition compared to the controls.</p><p><strong>Conclusion: </strong><i>L. pentosus</i> WSL5 and <i>T. ciferri</i> RSY53 can be considered potential starter cultures for the fermentation of functional foods aimed at supporting cardiovascular health.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 2","pages":"183-194"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144700877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional content and renoprotective potential of miracle tree (<i>Moringa oleifera</i>).","authors":"Vibhuti Sharma, Gaytri Mahajan, Reena Gupta","doi":"10.5114/bta/204529","DOIUrl":"https://doi.org/10.5114/bta/204529","url":null,"abstract":"<p><p>Kidney disease is a significant global health issue. This review explores the causes of renal illness, the therapeutic properties of the angiosperm <i>Moringa oleifera</i>, and its potential effectiveness in kidney disease management. In chronic kidney disease, inflammatory processes and immune cell activation lead to excessive free radical production, resulting in oxidative stress due to diminished antioxidant capacity. <i>M. oleifera</i> possesses diverse health benefits, particularly its ability to enhance antioxidant defenses. Extracts from its stem, seed, and leaf powders have been shown to increase the activity of key antioxidant enzymes, including glutathione, superoxide dismutase, and catalase. Additionally, <i>M. oleifera</i> modulates inflammation by reducing the activity of TNF-α, COX-2, and other pro-inflammatory cytokines. This review provides insights into the pharmacological and therapeutic potential of <i>M. oleifera</i>, highlighting its promise in the development of novel treatments for kidney-related disorders. Moreover, its bioactive compounds may contribute to renal tissue regeneration and protection against nephrotoxic agents.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 2","pages":"223-239"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144700882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of <i>UGPase</i> and <i>SPP</i> genes in <i>Nicotiana tabacum</i> leaves causes accelerated plant development and increased biomass.","authors":"Magdalena Rakoczy, Jan Podkowinski, Marek Figlerowicz","doi":"10.5114/bta/201460","DOIUrl":"10.5114/bta/201460","url":null,"abstract":"<p><strong>Background: </strong>Sucrose phosphate phosphatase (<i>SPP</i>) and uridine diphosphate-glucose pyrophosphorylase (<i>UGPase</i>) genes were overexpressed in <i>Nicotiana tabacum</i> to enhance the efficiency of the photosynthesis-related sucrose synthesis pathway, the primary route for incorporating newly fixed carbon into plant metabolism.</p><p><strong>Materials and methods: </strong>To target transgene expression specifically to the leaves, the <i>Chrysanthemum x morifolium</i> Rubisco small subunit promoter was used.</p><p><strong>Results: </strong>Transgenic plants overexpressing <i>HvSPP</i> and <i>HvUGPase</i> exhibited high transgene expression in the leaves, exceeding those of the corresponding <i>N. tabacum</i> genes by more than tenfold. These plants grew faster and entered the generative phase earlier than control plants, without showing any other developmental abnormalities. By the end of the generative phase, transgenic plants had greater dry mass and contained a higher proportion of carbohydrates than the control group. In result, they accumulated 14.9-17.5% more energy in the aboveground parts compared to reference plants.</p><p><strong>Conclusions: </strong>The high leaf specificity of the <i>C. x morifolium</i> Rubisco small subunit promoter was confirmed, indicating that transgene activity in leaves was effectively separated from its effects on metabolism in non-photosynthetic tissues. Overexpression of <i>HvUGPase</i> and <i>HvSPP</i> under this promoter accelerated plant growth and development, ultimately increasing biomass. These characteristics are particularly advantageous for energy crops grown as after-crops or in regions with short growing seasons.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 1","pages":"31-48"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Second brain: reviewing the gut microbiome's role in lifestyle diseases.","authors":"Hindol Ray, Janatum Khatum, Srijan Haldar, Priyanka Bhowmik","doi":"10.5114/bta/195495","DOIUrl":"10.5114/bta/195495","url":null,"abstract":"<p><p>The recent COVID-19 pandemic has highlighted another silent pandemic: lifestyle diseases. Conditions, such as cardiovascular diseases, anxiety, and type 2 diabetes (T2D), are increasingly becoming public health threats, affecting even younger populations worldwide. In recent years, extensive research has uncovered the pivotal role of the human gut microbiome in various aspects of human physiology, including metabolism, cellular homeostasis, immune defense, and disease development. The gut microbiome, often referred to as the \"second brain,\" is now recognized as a key player in health and disease. Lifestyle factors such as diet, mental health, stress, exercise, and others significantly influence the composition of the gut microbiome. Imbalances in this composition, termed \"dysbiosis,\" have been linked to a wide range of diseases, including cancer, cardiovascular diseases, obesity, T2D, asthma, and neurological disorders like Alzheimer's and Parkinson's disease. These findings underscore the profound influence of gut microbiome health on overall well-being. A working understanding of the gut microbiome's composition and its impact on disease processes is crucial for the advancement of personalized or precision medicine. This review article aims to explore recent advancements in the field, shedding light on how the gut microbiome contributes to the development and prognosis of lifestyle diseases.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 1","pages":"103-122"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical and transcriptomic profiling analysis of drought tolerant related genes in ML 82-2 and ML 125-2 rice mutant lines.","authors":"Jun Ying Low, Rhun Yian Koh, Hussein Sobri, Ahmad Faiz, Anna Pick Kiong Ling","doi":"10.5114/bta/200704","DOIUrl":"10.5114/bta/200704","url":null,"abstract":"<p><strong>Background: </strong>Backcrossing of gamma-irradiated IS21 with MR220 has generated progenies (ML 82-2 and ML 125-2) with improved traits. However, studies on these new mutant lines remain limited. This study aimed to determine and compare the biochemical characteristics and transcriptomic profiles of drought-tolerance-related genes in ML 82-2 and ML 125-2, as well as in the parent lines, IS21 and MR220.</p><p><strong>Materials and methods: </strong>Seeds were germinated for 14 days under a controlled photoperiod (16 h light and 8 h darkness) at a constant temperature of 25 ± 2°C. Biochemical analyses, including total soluble protein content, specific peroxidase activity (SPA), chlorophyll content, and proline content, were conducted. Transcriptomic profiling was performed using STRING and gene ontology (GO) enrichment analysis.</p><p><strong>Results: </strong>ML 82-2 exhibited the highest SPA, which was significantly different from that of MR220 and IS21, as well as significantly different total soluble protein content. However, ML 82-2 did not significantly differ from MR220 in chlorophyll and proline content. Drought stress-responsive genes <i>Os01g0124401, Os08g0473900,</i> and <i>Os08g0518800</i> were identified in ML 82-2. Conversely, ML 125-2 displayed total soluble protein content and SPA similar to IS21, while chlorophyll and proline content were not significantly different from those of MR220. Drought stress-responsive genes <i>Os10g0471100, Os01g0197100,</i> and <i>Os11g0701400</i> were identified in ML 125-2.</p><p><strong>Conclusions: </strong>ML 82-2 demonstrated improved SPA, whereas ML 125-2 exhibited enhanced total soluble protein content. The identified genes in both mutant lines are associated with drought resistance, with most sharing a similar genomic profile with MR220. These findings contribute to plant physiology studies and stress-responsive gene discovery in rice.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 1","pages":"13-30"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12093092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}