Biotechnologia最新文献

Overexpression of UGPase and SPP genes in Nicotiana tabacum leaves causes accelerated plant development and increased biomass. 烟草叶片中UGPase和SPP基因的过表达导致植株发育加快，生物量增加。

Biotechnologia Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.5114/bta/201460

Magdalena Rakoczy, Jan Podkowinski, Marek Figlerowicz

{"title":"Overexpression of UGPase and SPP genes in Nicotiana tabacum leaves causes accelerated plant development and increased biomass.","authors":"Magdalena Rakoczy, Jan Podkowinski, Marek Figlerowicz","doi":"10.5114/bta/201460","DOIUrl":"10.5114/bta/201460","url":null,"abstract":"Background: Sucrose phosphate phosphatase (SPP) and uridine diphosphate-glucose pyrophosphorylase (UGPase) genes were overexpressed in Nicotiana tabacum to enhance the efficiency of the photosynthesis-related sucrose synthesis pathway, the primary route for incorporating newly fixed carbon into plant metabolism.Materials and methods: To target transgene expression specifically to the leaves, the Chrysanthemum x morifolium Rubisco small subunit promoter was used.Results: Transgenic plants overexpressing HvSPP and HvUGPase exhibited high transgene expression in the leaves, exceeding those of the corresponding N. tabacum genes by more than tenfold. These plants grew faster and entered the generative phase earlier than control plants, without showing any other developmental abnormalities. By the end of the generative phase, transgenic plants had greater dry mass and contained a higher proportion of carbohydrates than the control group. In result, they accumulated 14.9-17.5% more energy in the aboveground parts compared to reference plants.Conclusions: The high leaf specificity of the C. x morifolium Rubisco small subunit promoter was confirmed, indicating that transgene activity in leaves was effectively separated from its effects on metabolism in non-photosynthetic tissues. Overexpression of HvUGPase and HvSPP under this promoter accelerated plant growth and development, ultimately increasing biomass. These characteristics are particularly advantageous for energy crops grown as after-crops or in regions with short growing seasons.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 1","pages":"31-48"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Second brain: reviewing the gut microbiome's role in lifestyle diseases. 第二大脑：回顾肠道微生物群在生活方式疾病中的作用。

Biotechnologia Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.5114/bta/195495

Hindol Ray, Janatum Khatum, Srijan Haldar, Priyanka Bhowmik

{"title":"Second brain: reviewing the gut microbiome's role in lifestyle diseases.","authors":"Hindol Ray, Janatum Khatum, Srijan Haldar, Priyanka Bhowmik","doi":"10.5114/bta/195495","DOIUrl":"10.5114/bta/195495","url":null,"abstract":"The recent COVID-19 pandemic has highlighted another silent pandemic: lifestyle diseases. Conditions, such as cardiovascular diseases, anxiety, and type 2 diabetes (T2D), are increasingly becoming public health threats, affecting even younger populations worldwide. In recent years, extensive research has uncovered the pivotal role of the human gut microbiome in various aspects of human physiology, including metabolism, cellular homeostasis, immune defense, and disease development. The gut microbiome, often referred to as the \"second brain,\" is now recognized as a key player in health and disease. Lifestyle factors such as diet, mental health, stress, exercise, and others significantly influence the composition of the gut microbiome. Imbalances in this composition, termed \"dysbiosis,\" have been linked to a wide range of diseases, including cancer, cardiovascular diseases, obesity, T2D, asthma, and neurological disorders like Alzheimer's and Parkinson's disease. These findings underscore the profound influence of gut microbiome health on overall well-being. A working understanding of the gut microbiome's composition and its impact on disease processes is crucial for the advancement of personalized or precision medicine. This review article aims to explore recent advancements in the field, shedding light on how the gut microbiome contributes to the development and prognosis of lifestyle diseases.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 1","pages":"103-122"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical and transcriptomic profiling analysis of drought tolerant related genes in ML 82-2 and ML 125-2 rice mutant lines. 水稻mml 82-2和mml 125-2突变系耐旱相关基因的生化和转录组学分析

Biotechnologia Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.5114/bta/200704

Jun Ying Low, Rhun Yian Koh, Hussein Sobri, Ahmad Faiz, Anna Pick Kiong Ling

{"title":"Biochemical and transcriptomic profiling analysis of drought tolerant related genes in ML 82-2 and ML 125-2 rice mutant lines.","authors":"Jun Ying Low, Rhun Yian Koh, Hussein Sobri, Ahmad Faiz, Anna Pick Kiong Ling","doi":"10.5114/bta/200704","DOIUrl":"10.5114/bta/200704","url":null,"abstract":"Background: Backcrossing of gamma-irradiated IS21 with MR220 has generated progenies (ML 82-2 and ML 125-2) with improved traits. However, studies on these new mutant lines remain limited. This study aimed to determine and compare the biochemical characteristics and transcriptomic profiles of drought-tolerance-related genes in ML 82-2 and ML 125-2, as well as in the parent lines, IS21 and MR220.Materials and methods: Seeds were germinated for 14 days under a controlled photoperiod (16 h light and 8 h darkness) at a constant temperature of 25 ± 2°C. Biochemical analyses, including total soluble protein content, specific peroxidase activity (SPA), chlorophyll content, and proline content, were conducted. Transcriptomic profiling was performed using STRING and gene ontology (GO) enrichment analysis.Results: ML 82-2 exhibited the highest SPA, which was significantly different from that of MR220 and IS21, as well as significantly different total soluble protein content. However, ML 82-2 did not significantly differ from MR220 in chlorophyll and proline content. Drought stress-responsive genes Os01g0124401, Os08g0473900, and Os08g0518800 were identified in ML 82-2. Conversely, ML 125-2 displayed total soluble protein content and SPA similar to IS21, while chlorophyll and proline content were not significantly different from those of MR220. Drought stress-responsive genes Os10g0471100, Os01g0197100, and Os11g0701400 were identified in ML 125-2.Conclusions: ML 82-2 demonstrated improved SPA, whereas ML 125-2 exhibited enhanced total soluble protein content. The identified genes in both mutant lines are associated with drought resistance, with most sharing a similar genomic profile with MR220. These findings contribute to plant physiology studies and stress-responsive gene discovery in rice.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 1","pages":"13-30"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12093092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of salinity on the morpho-biochemical traits of hydroponically cultivated Spinacia oleracea L. 盐度对水培菠菜形态生化性状的影响。

Biotechnologia Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.5114/bta/202318

Archana A Naik, Shekhar D Tidke, Mahadev R Chambhare, Ravindra D Bansode, Panchshila S Kabnoorkar

{"title":"Impact of salinity on the morpho-biochemical traits of hydroponically cultivated Spinacia oleracea L.","authors":"Archana A Naik, Shekhar D Tidke, Mahadev R Chambhare, Ravindra D Bansode, Panchshila S Kabnoorkar","doi":"10.5114/bta/202318","DOIUrl":"10.5114/bta/202318","url":null,"abstract":"Background: Spinacia oleracea, a nutrient-dense vegetable composed of 91% water, 4% starch, and 3% protein, is a staple in the Indian diet. However, salinity stress can hinder its growth. This study examines the effects of salinity on the morpho-biochemical traits of spinach in a hydroponic system.Materials and methods: Spinach seeds were sown on coco peat, and after 10 days, the seedlings were transferred to the hydroponic setup. The plants were treated with salt concentrations of ECe 0, 4.0, 6.0, 8.0, 10.0, and 12.0 ds/m, and various morpho-biochemical parameters were assessed at 10-day intervals.Results: In the control group, seed germination was 59.6 ± 1.45%, while increasing salt concentrations (4 to 10 dS/m) progressively reduced germination (50 ± 1.2% to 14 ± 2%). Root and shoot lengths (root: 14.16 ± 0.19 cm; shoot: 4.23 ± 0.28 cm at 4 dS/m), relative water content (47.5 ± 0.43% to 32.1 ± 0.86%), and leaf surface area (25.03 ± 0.26 to 9 ± 0.12 cm2) all declined with increasing salinity. Conversely, proline content (0.055 ± 0 to 0.12 ± 0 μg/ml) and antioxidant enzyme activity (SOD: 1.83±0.04/g protein; APX: 0.53 ± 0.1/mg protein; CAT: 0.0054 ± 0/mg protein) increased compared to the control. However, chlorophyll content (3.73 ±0.02 to 1.95 ± 0.03 mg/g at 12 dS/m) and protein content (0.13 ± 0 at 4 dS/m to 0.054 ± 0 μg/ml at 12 dS/m) decreased with rising salinity.Conclusions: Therefore, it is concluded that spinach grown hydroponically can tolerate salt stress up to ECe 6.0 dS/m after 30 days of treatments, and more increased (8.0 to 12 dS/m) salt concentration that adversely affects overall morpho-biochemical performance.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 1","pages":"49-62"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expectations and limitations of biotechnology in the perspective of 2050. 展望2050年生物技术的发展前景与局限性。

Biotechnologia Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.5114/bta/203189

Tomasz Twardowski

引用次数: 0

Evaluation of the physical, chemical, antioxidant, and antibacterial properties of Camellia oleifera Abel. seed oil. 油茶的物理、化学、抗氧化和抗菌性能评价。籽油。

Biotechnologia Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.5114/bta/195496

Pham My Hao, Luu Thao Nguyen, Tran Thi Mai Anh, Le Pham Tan Quoc

{"title":"Evaluation of the physical, chemical, antioxidant, and antibacterial properties of Camellia oleifera Abel. seed oil.","authors":"Pham My Hao, Luu Thao Nguyen, Tran Thi Mai Anh, Le Pham Tan Quoc","doi":"10.5114/bta/195496","DOIUrl":"10.5114/bta/195496","url":null,"abstract":"Background: Camellia seeds are rich in oil and contain fatty acids that offer significant health benefits.Materials and methods: This study aimed to characterize Camellia oleifera seeds and their oil. Physical properties of the seeds, including 1000-seed weight, density, moisture content, specific gravity, and angle of repose, were determined. Camellia seed oil was extracted using the pressing method, and various physicochemical and biological properties of the oil-such as density, color, acid, peroxide, and saponification values, as well as antioxidant and antibacterial activities-were assessed.Results: Pressing the oil at 140oC yielded optimal results, achieving a recovery efficiency of 21.67%. Gas chromatography-mass spectrometry identified seven fatty acid components in the oil, with oleic acid (71.03%) being the most abundant. The antioxidant capacity of the oil was evaluated using a DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, yielding an IC50 value of 265.8 mg/mL. However, the oil exhibited no inhibitory effects against four bacterial strains (Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, and Staphylococcus aureus).Conclusions: These findings highlight the potential of Camellia seed oil in food technology, particularly as an alternative to commercial cooking oils.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 1","pages":"5-12"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico screening and identification of CTL and HTL epitopes in the secreted virulence factors of Mycobacterium tuberculosis. 结核分枝杆菌分泌毒力因子中CTL和HTL表位的筛选与鉴定。

Biotechnologia Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.5114/bta/201461

Edward Kevin B Bragais, Francisco M Heralde, Kim Claudette J Fernandez, Salvador Eugenio C Caoili, Leana Rich Herrera-Ong

{"title":"In silico screening and identification of CTL and HTL epitopes in the secreted virulence factors of Mycobacterium tuberculosis.","authors":"Edward Kevin B Bragais, Francisco M Heralde, Kim Claudette J Fernandez, Salvador Eugenio C Caoili, Leana Rich Herrera-Ong","doi":"10.5114/bta/201461","DOIUrl":"10.5114/bta/201461","url":null,"abstract":"Background: Mycobacterium tuberculosis (MTb) is a highly infectious pathogen and a global health threat due to its resilient cell wall and immune evasion strategies. Despite the availability of the antituberculosis Bacille Calmette-Guérin (BCG) vaccine, its efficacy varies (0%-80%) and gradually decreases over time. This study aimed to identify cytotoxic T-lymphocyte (CTL) and helper T-lymphocyte (HTL) epitopes in MTb secretory proteins using immunoinformatics tools.Materials and methods: The Protein Variability Server was used to identify highly conserved sequences, and epitope population coverage was estimated for the Southeast Asian (SEA) region. Selected epitopes were also docked to their major histocompatibility complex alleles.Results: Five secretory proteins critical to MTb pathogenesis and virulence were identified as antigenic (antigenicity score > 0.4). Predicted epitopes had IC50 values ≤ 500 nM, indicating strong binding affinity, with an estimated 94% population coverage in SEA. All candidate epitopes were highly conserved (Shannon index ≤ 0.1) and showed no significant sequence similarity to human proteins, allergens, or toxic peptides. Docking analysis confirmed favorable binding to their corresponding HLA alleles, as indicated by low Gibbs free energy change (ΔG) values and dissociation constants (K D nM).Conclusions: Overall, this study identified immunoactive CTL and HTL epitopes that could serve as promising candidates for future antiTB vaccine development. Further in vitro and in vivo studies are required to validate these preliminary findings.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 1","pages":"63-76"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutting-edge AI tools revolutionizing scientific research in life sciences. 尖端的人工智能工具革新了生命科学的科学研究。

Biotechnologia Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.5114/bta/200803

Katarzyna Lorenc-Kukuła

引用次数: 0

In silico evaluation, characterization, and in vitro anticancer activity of curcumin-nimbin loaded nanoformulation in HCT-116 cell lines. 姜黄素-宁宾负载纳米制剂在HCT-116细胞系中的体外抗癌活性

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145256

Arumugam Madeswaran, Selvam Tamilazhagan, Sellappan Mohan

{"title":"In silico evaluation, characterization, and in vitro anticancer activity of curcumin-nimbin loaded nanoformulation in HCT-116 cell lines.","authors":"Arumugam Madeswaran, Selvam Tamilazhagan, Sellappan Mohan","doi":"10.5114/bta.2024.145256","DOIUrl":"10.5114/bta.2024.145256","url":null,"abstract":"Colorectal cancer is one of the most prevalent malignancies worldwide and a leading cause of mortality. Chemotherapy medications are often limited in use due to issues like drug resistance, P-glycoprotein efflux, and relapse of chemotherapy. In this study, we formulated a nanosuspension with curcumin and nimbin to address these limitations and assessed its anticancer potential using in silico molecular docking and in vitro MTT assay.Methods: In silico docking and ADMET analyses targeted proteins implicated in colorectal cancer, with doxorubicin as the standard. The docking studies were conducted using AutoDock 4.2, while in vitro anticancer activity was assessed through the MTT assay in HCT 116 cell lines.Results: In silico docking of curcumin and nimbin showed significant interactions with target proteins compared to the standard. ADMET analysis indicated favorable Caco-2 permeability and intestinal absorption of the selected phytoconstituents. The MTT assay demonstrated concentration-dependent cell viability inhibition in HCT 116 cell lines treated with the nanosuspension, with an IC50 value of 30%.Conclusion: The curcumin-nimbin loaded nanosuspension demonstrated promising anticancer activity against HCT 116 cell lines in both in silico and in vitro studies. Further studies are required to evaluate the anticancer effect of curcumin-nimbin loaded nanosupension through clinical and preclinical studies for the progress of potential formulation in the treatment of colorectal cancer.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"355-365"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm architecture and dynamics of the oral ecosystem. 口腔生态系统的生物膜结构与动力学。

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145259

Rina Rani Ray

{"title":"Biofilm architecture and dynamics of the oral ecosystem.","authors":"Rina Rani Ray","doi":"10.5114/bta.2024.145259","DOIUrl":"10.5114/bta.2024.145259","url":null,"abstract":"The oral cavity, being a nutritionally enriched environment, has been proven to be an ideal habitat for biofilm development. Various microenvironments, including dental enamel, supra- and subgingival surfaces, salivary fluid, and the dorsal surface of the tongue, harbor diverse microbes. These biofilms typically consist of four major layers. Depending on the food, age, clinical state, and lifestyle of the patient, the microbial growth dynamics in oral biofilm varies significantly. The presence of pathogenic bacteria that disrupt the normal floral composition of the oral cavity can lead to plaque biofilm formation, which is a precursor to various diseases. Noteworthy pathogenic bacteria, such as Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans, often initiate biofilm formation. Undiagnosed and untreated oral biofilm can lead to severe diseases like periodontitis and eventual tooth loss. Therefore, studying the architecture and dynamics of oral biofilms is essential and can be achieved through image analysis and modern technologies, such as AI-enabled technologies and surface topography-adaptive robotic superstructures.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"395-402"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0