Biotechnologia最新文献_第2页

Induction of an immune response by a nonreplicating adenoviruses-based formulation versus a commercial pseudo-SARS-CoV-2 vaccine. 以非复制腺病毒为基础的制剂与商用伪 SARS-CoV-2 疫苗相比可诱导免疫反应。

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141805

Joanna Baran, Łukasz Kuryk, Mariangela Garofalo, Katarzyna Pancer, Magdalena Wieczorek, Michalina Kazek, Monika Staniszewska

{"title":"Induction of an immune response by a nonreplicating adenoviruses-based formulation versus a commercial pseudo-SARS-CoV-2 vaccine.","authors":"Joanna Baran, Łukasz Kuryk, Mariangela Garofalo, Katarzyna Pancer, Magdalena Wieczorek, Michalina Kazek, Monika Staniszewska","doi":"10.5114/bta.2024.141805","DOIUrl":"https://doi.org/10.5114/bta.2024.141805","url":null,"abstract":"Screening for effective vaccines requires broad studies on their immunogenicity in vitro and ex vivo . We used a PBMC-based system to assess changes in CD4+ T cells, CD8+ T cells, and CD19+ B cells upon stimulation with different combinations of antigens and adjuvants. We studied the activation mechanism using flow cytometry and two different adenoviral adjuvants characterized by the presence or absence of costimulatory ligands for the ICOS and CD40 receptors. Our studies identified the cellular targets and molecular mechanisms driving ongoing switched-antibody diversification. Class-switched memory B cells were the main precursor cells (95.03% ± 0.38 vs. mock 82.33% ± 0.45, P < 0.05) after treatment with the immunogenic formula: adenovirus armed (MIX1) or not (MIX2) with the ICOS and CD40 ligand, the recombinant receptor binding domain (rRBD), and Lentifect™ SARS-CoV-2 spike-pseudotyped lentivirus (GeneCopoeia, USA). Bcell class-switching towards the IgG+IgM+- positive phenotypes was noted (~50-fold increase vs. mock, P < 0.05). A significant increase was observed in the CD8+TEM population of the MIX1 (~2-fold, P < 0.05) and MIX2 (~4.7-fold, P < 0.05) treated samples. CD8+TEMRA increased when PBMCs were treated with MIX2 (9.63% ± 0.90, P < 0.05) vs. mock (2.63% ± 1.96). Class-switched memory B cells were the dominant antigen-specific cells in primary reactions. We indicated a correlation between the protection offered by vaccine regimens and their ability to induce high frequencies of multifunctional T cells.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"263-272"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of some explants for callus induction and plantlet regeneration in Solanum lycopersicum L. under treatment of different plant growth regulators. 在不同植物生长调节剂的作用下，某些外植体诱导番茄茄属植物胼胝体和小植株再生的潜力

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141803

Anjana Kumari, Avinash K Nagpal, Jatinder K Katnoria

{"title":"Potential of some explants for callus induction and plantlet regeneration in Solanum lycopersicum L. under treatment of different plant growth regulators.","authors":"Anjana Kumari, Avinash K Nagpal, Jatinder K Katnoria","doi":"10.5114/bta.2024.141803","DOIUrl":"https://doi.org/10.5114/bta.2024.141803","url":null,"abstract":"Plant growth regulators (PGRs) control signaling networks and developmental processes involved in plant responses to various biotic and abiotic stresses, making it crucial to study PGRs in vitro. The protocol for micropropagation of Solanum lycopersicum L., following callus induction and regeneration through explants such as internodal segments, leaves, and nodal segments, was established during the present study. Explants were inoculated on Murashige and Skoog (MS) medium supplemented with different plant growth regulators like BA (6-benzylaminopurine), 2,4-D (2,4-dichlorophenoxyacetic acid), BA + 2,4-D, IAA (Indoleacetic acid), IBA (Indolebutyric acid), and NAA (Naphthaleneacetic acid). It was observed that among all explants, the nodal segment showed maximum callus induction (83.33%) and multiplication (86.67%) at 0.25 mg/l of 2,4-D; the highest shoot number (3.33) at 0.5 mg/l of IAA + 0.5 mg/l of BA; the greatest shoot length (7.57 cm) at 0.75 mg/l of BA; root induction (80.95%), root number (21.88), and root length (10.62 cm) at 1 mg/l of IAA. Additionally, the maximum fresh weight (2.448 g) was observed at 0.5 mg/l of BA, while the highest dry weight (0.172 g) and dry matter content (14.25%) were noted at 1 mg/l of BA + 1 mg/l of 2,4-D for the internodal segment. Results of the present study revealed that among different explants, the best response was given by nodal segments, followed by internodal segments. Among the different PGRs, 2,4-D resulted in the highest callus induction and multiplication percentage.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"227-247"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on xylanase sources, classification, mode of action, fermentation processes, and applications as a promising biocatalyst. 综述木聚糖酶的来源、分类、作用方式、发酵过程以及作为一种有前途的生物催化剂的应用。

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141806

Tariku Abena, Addis Simachew

{"title":"A review on xylanase sources, classification, mode of action, fermentation processes, and applications as a promising biocatalyst.","authors":"Tariku Abena, Addis Simachew","doi":"10.5114/bta.2024.141806","DOIUrl":"https://doi.org/10.5114/bta.2024.141806","url":null,"abstract":"The utilization of hydrolytic enzymes in various industrial processes worldwide has gained more attention than chemical catalysts due to the high selectivity of enzymes, their ease of control, and their negligible environmental impact, as they produce very small amounts of byproducts. Xylanase is one such enzyme that catalyzes the breakdown of the β-1,4 linkage of xylan, the second most abundant renewable heteropolysaccharide and hemicellulosic constituent of the plant cell wall. Naturally, xylanase can be obtained from various sources such as mollusks, insects, plants, animals, and various microorganisms (bacteria, fungi, yeast, and algae). The utilization of xylanase could greatly improve the overall economics of processing lignocellulosic materials for the generation of monosaccharides, liquid fuels, and chemicals. Microbial xylanase is suitable for applications in food and feed, paper and pulp, textile, pharmaceutical, and biorefining industries. It has gained global attention due to its substrate specificities, biochemical properties, and various biotechnological applications. This review focuses on xylanase production, sources, fermentation processes, modes of action, purification methods, and applications in various industries.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"273-285"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the antioxidant, antidiabetic, and antibacterial potential of postbiotic compounds derived from Lactiplantibacillus plantarum O7S1. 探索从植物乳杆菌 O7S1 中提取的后生物化合物的抗氧化、抗糖尿病和抗菌潜力。

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141802

Nadia Aliouche, Mohamed Sifour, Houria Ouled-Haddar

{"title":"Exploring the antioxidant, antidiabetic, and antibacterial potential of postbiotic compounds derived from Lactiplantibacillus plantarum O7S1.","authors":"Nadia Aliouche, Mohamed Sifour, Houria Ouled-Haddar","doi":"10.5114/bta.2024.141802","DOIUrl":"https://doi.org/10.5114/bta.2024.141802","url":null,"abstract":"Probiotic bacteria are distinguished by their ability to produce various functional postbiotic metabolites. Therefore, this study aimed to explore the in vitro antioxidant, antidiabetic, and antibacterial properties of two postbiotics generated by Lactiplantibacillus plantarum O7S1 (Lpb. plantarum O7S1) during the fermentation process: cell-free supernatant (CFS) and exopolysaccharides (EPS). The antioxidant potential of these postbiotics was assessed using various radical scavenging assays and ferric-reducing antioxidant potential. The antidiabetic activity was evaluated through α-amylase inhibitory assays, while antibacterial activity was determined using agar well diffusion assays. The results of the present study revealed that CFS exhibited significant antioxidant and antidiabetic efficacy in contrast to EPS (P < 0.001). Specifically, CFS displayed remarkable scavenging ability against DPPH, hydroxyl, and superoxide radicals, with inhibition rates of 88.78, 78.91, and 34.85%, respectively, while EPS showed comparatively lower inhibition rates. Additionally, CFS demonstrated higher reducing activity (0.30 optical density units at 700 nm) and potent α-amylase inhibitory activity (95.87%) compared to EPS (67.17%) (P < 0.001). The agar well diffusion assay reported that CFS showed significant antimicrobial activity against both Gram-positive and Gram-negative pathogens, while no activity was observed with EPS. Furthermore, carbohydrate fermentation analysis indicated the strain's ability to metabolize various carbohydrates and their derivatives, potentially enhancing digestive health. These findings suggest that both CFS and EPS exhibit promising hypoglycemic, antioxidant, and antibacterial properties, making them potential candidates for incorporation into functional foods and pharmaceuticals aimed at preventing oxidative damage, diabetes, and pathogenic bacterial infections.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"215-225"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical composition, antioxidant, antibacterial, and enzyme inhibitory activities of organic extracts from flower buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry. Cleistocalyx operculatus (Roxb.) Merr. et Perry.

Biotechnologia Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.139753

Doan Thien Thanh, Vo Kieu Oanh, Hoang Chinh Nguyen, Luong Thi My Ngan, Tran Trung Hieu

{"title":"Phytochemical composition, antioxidant, antibacterial, and enzyme inhibitory activities of organic extracts from flower buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry.","authors":"Doan Thien Thanh, Vo Kieu Oanh, Hoang Chinh Nguyen, Luong Thi My Ngan, Tran Trung Hieu","doi":"10.5114/bta.2024.139753","DOIUrl":"10.5114/bta.2024.139753","url":null,"abstract":"Cleistocalyx operculatus flower buds have been widely used in traditional medicine because of their rich content of bioactive constituents. In this study, we obtained seven solvent extracts from the flower buds and evaluated their total phenolic (TPC), flavonoid (TFC), tannin (TTC), triterpenoid saponin (TSC), and alkaloid (TAC) contents. We assessed antioxidant activities using the DPPH assay and also looked at antimicrobial and enzyme inhibitory effects. The water extract possessed the highest TPC (328.9 mg GAE/g extract), followed by ethanol, methanol, and hexane extracts (85.4-101.5 mg GAE/g extract). Chloroform, butanol, ethyl acetate, and ethanol extracts had high TSCs (245.4-287.2 mg OAE/g extract). The hexane extract was richest in TTC and TFC (32.7 mg CE/g extract and 81.1 mg QE/g extract, respectively). Ethanol and methanol extracts exhibited the strongest antioxidant activities (IC50 values of 25.2 and 30.3 μg/ml, respectively), followed by the water extract (IC50 of 40.2 μg/ml). The hexane extract displayed the most growth-inhibitory activity against Helicobacter pylori ATCC51932 and ATCC43504 strains and Salmonella enterica serovar Typhimurium ATCC13311 (MIC values of 0.06, 0.13, and 0.4 mg/ml, respectively). Moreover, the hexane extract revealed the strongest inhibition of H. pylori urease activity (IC50 of 4.51 μg/ml), whereas the water and methanol extracts had potent inhibitory effects on α-glucosidase activity (IC50 values of 9.9 and 15.1 μg/ml, respectively). These flower bud extracts could be used for health protection, especially in preventing bacterial infections and inhibiting enzymes associated with various human diseases. Further investigation into the application of C. operculatus flower buds in the food and pharmaceutical industries is necessary.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 2","pages":"137-147"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11231999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Spirulina platensis biomass on the viability of Lactobacillus delbrueckii subsp. bulgaricus strain during the freeze-drying process. 冷冻干燥过程中螺旋藻生物量对保加利亚乳杆菌亚种活力的影响

Biotechnologia Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.139751

Ivo Ganchev

引用次数: 0

Secondary metabolites, spectra characterization, and antioxidant correlation analysis of the polar and nonpolar extracts of Bryophyllum pinnatum (Lam) Oken. 奥肯（Bryophyllum pinnatum (Lam) Oken）极性和非极性提取物的次生代谢物、光谱特征和抗氧化相关性分析。

Biotechnologia Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.139752

Chinyere E Okafor, Ikechukwu K Ijoma, Chiamaka A Igboamalu, Chinaza E Ezebalu, Chukwuemeka F Eze, Jessica C Osita-Chikeze, Chisom E Uzor, Adaugo L Ekwuekwe

{"title":"Secondary metabolites, spectra characterization, and antioxidant correlation analysis of the polar and nonpolar extracts of Bryophyllum pinnatum (Lam) Oken.","authors":"Chinyere E Okafor, Ikechukwu K Ijoma, Chiamaka A Igboamalu, Chinaza E Ezebalu, Chukwuemeka F Eze, Jessica C Osita-Chikeze, Chisom E Uzor, Adaugo L Ekwuekwe","doi":"10.5114/bta.2024.139752","DOIUrl":"10.5114/bta.2024.139752","url":null,"abstract":"Oxidative stress-related pathologies have guided the scientific community into delving into natural product-based research on plant-based metabolites. Plant secondary metabolites serve as a valid alternative in managing oxidative stress-related pathologies. In this study, we present the secondary metabolite constituents of the polar extract (PE) and nonpolar extract (NPE) from the leaves of Bryophyllum pinnatum. These constituents were determined through qualitative and quantitative phytochemical screening. The functional groups and structures of these metabolites were determined based on FTIR and GC-MS experiments, respectively. Antioxidant and free radical scavenging (FRS) activities were determined using standard methods, including phosphomolybdenum, FRAP, DPPH, HRSA, and reducing power assays, with comparisons made to the ascorbic acid (AA) standard. Through Pearson correlation analysis, we estimated the relationship between antioxidant and FRS activities. The DPPH results revealed IC50s of 380.104 ± 0.001, 16.763 ± 0.001, and 7.684 ± 0.003 μg/ml for NPE, AA, and PE, respectively, indicating a trend of PE > AA > NPE. However, all other experiments showed a trend of AA > PE > NPE in antioxidant and FRS activities. These results showed the potential antioxidant and FRS properties of both PE and NPE. Additionally, the correlation analysis indicated a strong positive correlation between the antioxidant and FRS activities of PE and NPE. The research results suggest high antioxidant and FRS activities of PE and validate the use of B. pinnatum in managing free radical-related pathologies.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 2","pages":"121-136"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11232000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological lactic acid production from low-cost renewable sources via anaerobic microbial processes. 通过厌氧微生物工艺利用低成本可再生资源生产乳酸的生物技术。

Biotechnologia Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.139757

Andriy Anta Kacaribu, Darwin

{"title":"Biotechnological lactic acid production from low-cost renewable sources via anaerobic microbial processes.","authors":"Andriy Anta Kacaribu, Darwin","doi":"10.5114/bta.2024.139757","DOIUrl":"10.5114/bta.2024.139757","url":null,"abstract":"Lactic acid (LA) production from microbial fermentation using low-cost renewable sources has emerged as an attractive alternative to the use of petroleum-based products. This approach not only offers sustainable solutions for waste management but also enables the production of value-added products in an eco-friendly manner. However, to make this approach economically viable, optimizing the production process for high yield, productivity, and purity while minimizing costs is crucial. To address these challenges, various approaches have been proposed, including the use of neutralizing agents, high cell density cultures, co-cultures, fed-batch fermentation, and product removal strategies. Overall, this review underscores the potential of microbial fermentation for LA production as a sustainable and cost-effective solution to meet the growing demand for eco-friendly products. Further optimization of fermentation processes and the development of new microbial strains and fermentation techniques are key to advancing this approach. The production of LA through microbial fermentation presents a sustainable and eco-friendly solution to the increasing demand for eco-friendly products. With continued innovation, we can expect to see a significant reduction in the environmental impact of industrial processes, coupled with a more cost-effective and high-purity source of lactic acid for various industries.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 2","pages":"179-194"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11232001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micropropagation of Dipcadi montanum (Dalz.) Baker (Asparagaceae): a rare scapigerous herb. Dipcadi montanum (Dalz.) Baker（天门冬科）的微繁殖：一种罕见的虎皮草。

Biotechnologia Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.139755

Sautrik Basu, Emadul Islam, Debraj Chakraborty

{"title":"Micropropagation of Dipcadi montanum (Dalz.) Baker (Asparagaceae): a rare scapigerous herb.","authors":"Sautrik Basu, Emadul Islam, Debraj Chakraborty","doi":"10.5114/bta.2024.139755","DOIUrl":"10.5114/bta.2024.139755","url":null,"abstract":"Dipcadi montanum (Dalz.) Baker (Asparagaceae) is a rare scapigerous herb endemic to the Western Ghats, a global biodiversity hotspot running parallel to the western coast of India. This study reports the development of a reproducible protocol for mass propagation of this underutilized geophyte using bulb scale and immature leaf base explants. Miniature bulblets were successfully induced from both types of explants after 4 and 8 weeks of culture on full-strength semisolid MS basal medium fortified with 3% sucrose and varying levels of BAP (4.4-17.7 μM) and TDZ (4.5-18.1 μM). The addition of 2.7 μM NAA further enhanced the rate of microbulb induction. Rooting of the 8-week-old bulblets, obtained from both explants, was achieved with more than 90% efficiency on liquid as well as agar-gelled half-strength MS basal medium fortified with varying levels of IBA (2.46-9.84 μM) and NAA (2.68-10.74 μM), with or without 2.32 μM Kinetin. More than 95% of the rooted plants survived the initial acclimatization process under controlled ex-vitro conditions, and a survival rate of over 80% was recorded after 4 weeks of transfer to greenhouse conditions. After a brief dormancy, the regenerants resumed growth in the postmonsoon season and exhibited morphological resemblance to the donor plant. Comparative cytological analysis between the donor and 15 randomly selected regenerants revealed a stable somatic count of 2n = 20.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 2","pages":"159-167"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11231995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetics of antibiotic resistance in methicillin-resistant Staphylococcus aureus (MRSA). 耐甲氧西林金黄色葡萄球菌（MRSA）的抗生素耐药性遗传学。

Biotechnologia Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.139756

Klementyna Marciniak, Agata Tyczewska, Kamilla Grzywacz

引用次数: 0