Biotechnologia最新文献_第2页

Gliclazide protects ionizing radiation-induced intestinal injury in mice by inhibiting oxidative stress and caspase-3. 格列齐特通过抑制氧化应激和caspase-3来保护电离辐射诱导的小鼠肠道损伤。

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145257

Soroush Arzani, Soghra Farzipour, Fereshteh Talebpour Amiri, Seyed Jalal Hosseinimehr

{"title":"Gliclazide protects ionizing radiation-induced intestinal injury in mice by inhibiting oxidative stress and caspase-3.","authors":"Soroush Arzani, Soghra Farzipour, Fereshteh Talebpour Amiri, Seyed Jalal Hosseinimehr","doi":"10.5114/bta.2024.145257","DOIUrl":"10.5114/bta.2024.145257","url":null,"abstract":"Gliclazide (GLZ), an oral antihyperglycemic medication, has additional beneficial effects, such as anti-inflammatory and antioxidant properties, besides lowering blood glucose levels. In this study, the radio-protective effect of GLZ was evaluated against ionizing radiation (IR)-induced intestinal injury in mice. Eight groups of mice were randomized as follows: control, GLZ (5, 10, and 25 mg/kg), IR (6 Gy), and IR + GLZ (at 5, 10, and 25 mg/kg). GLZ was administered to the mice for eight consecutive days, after which they were exposed to X-rays at a single dose of 6 Gy. After irradiation, biochemical parameters, immunohistochemical, and histological examinations were conducted on the ileum of the mice. IR exposure increased the levels of malondialdehyde and protein carbonyl, while glutathione levels, as oxidative stress biomarkers, decreased. Apoptosis in ileum tissues was also assessed. Furthermore, histopathological changes were observed in the irradiated mice. GLZ treatment significantly mitigated these changes. The administration of GLZ resulted in a marked decrease in caspase-3 immunoreactivity in the ileum of irradiated mice. This preclinical study exhibited that GLZ has a radioprotective effect against intestinal injury by inhibiting oxidative stress and apoptosis.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"367-376"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin nanoparticles as a therapeutic approach: pharmacological actions and potential applications in therapy. 槲皮素纳米颗粒作为一种治疗方法：药理作用和潜在的治疗应用。

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145258

Reham Farouk El-Sayed Baiomy

{"title":"Quercetin nanoparticles as a therapeutic approach: pharmacological actions and potential applications in therapy.","authors":"Reham Farouk El-Sayed Baiomy","doi":"10.5114/bta.2024.145258","DOIUrl":"10.5114/bta.2024.145258","url":null,"abstract":"The utilization of quercetin nanoparticles as a novel therapeutic strategy has garnered significant attention in recent years. These nanoparticles offer a unique approach to enhancing delivery and effectiveness while overcoming the drawbacks of quercetin. By exploiting the advantages of nanotechnology, such as increased stability and improved bioavailability, quercetin nanoparticles hold significant potential for developing innovative treatments across various medical fields. Quercetin nanoparticles have emerged as an indispensable component in numerous pharmaceutical and medicinal formulations. They are recognized for their anticancer, antitumor, anti-inflammatory, and antidiabetic properties, making them valuable in addressing allergic reactions, metabolic disorders, inflammatory disorders, cardiovascular diseases, and arthritis. From a pharmacological perspective, quercetin nanoparticles have demonstrated beneficial effects against Alzheimer's disease, primarily through their inhibitory impact on acetylcholinesterase. Furthermore, these nanoparticles have been scientifically documented to possess antioxidant, anticarcinogenic, hepatoprotective, and cytotoxic activities. This comprehensive review aims to explore the pharmacokinetics and biological activities associated with quercetin nanoparticles. It also highlights their potential as therapeutic agents in treating a wide range of diseases, including Alzheimer's disease, cancer, and neurodegenerative disorders.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"377-393"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of antioxidant, antimicrobial, and enzymatic properties of thermophilic cyanobacteria extracts. 嗜热蓝藻提取物的抗氧化、抗菌和酶促特性研究。

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145253

Aytan Fataliyeva, Meral Yilmaz Cankilic, Nalan Yilmaz Sariozlu

{"title":"Investigation of antioxidant, antimicrobial, and enzymatic properties of thermophilic cyanobacteria extracts.","authors":"Aytan Fataliyeva, Meral Yilmaz Cankilic, Nalan Yilmaz Sariozlu","doi":"10.5114/bta.2024.145253","DOIUrl":"10.5114/bta.2024.145253","url":null,"abstract":"Background: The present study investigated the antioxidant, antimicrobial, and partial enzymatic properties of 52 thermophilic cyanobacteria isolates in vitro.Materials and methods: The DPPH scavenging method was applied to test the antioxidant potential of isolates' methanol extracts. Agar block diffusion and agar well diffusion methods were used to evaluate the antimicrobial activity and measured in milimeters. The measurement of enzyme activity was carried out by a modification of the agar block method by the growth of the cyanobacteria.Results: Among the cyanobacterial extracts, strain 37 (0.78 ± 0.055 mg/ml) showed an IC50 value close to ascorbic acid (0.22 ± 0.04 mg/ml), indicating that it has a specific antioxidant source. Isolate G13 was shown to have the strongest antimicrobial activity against Micrococcus luteus NRRL B-4375 in the agar well diffusion method. In addition, the ability to produce enzymes was determined in isolate G1 (25 ± 5.66 mm), which had the highest cellulase activity at pH 8, and isolate K42 (22 ± 0.71 mm), which had the highest lipase activity at pH 7.Conclusion: When percent inhibition and IC50 values were examined, it was found that cyanobacterial methanol extracts had moderate and low scavenging activity in comparison to the standard antioxidant ascorbic acid. In a study using the agar well diffusion method, the activity of cyanobacterial extracts against test bacteria was observed. In light of the results obtained, it is believed that the isolates exhibited lipase and cellulase (pH 7 and 8) enzyme activity at both pH levels and have potential for industrial use.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"311-323"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First identification report for amino acid composition of red algae Gracilaria spp. obtained from Central Java, Indonesia. 印度尼西亚中爪哇红藻紫菜属（Gracilaria spp.）氨基酸组成首次鉴定报告。

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145254

Klara Kharisma Bunga Chandra, Tri Rini Nuringtyas, Tri Joko Raharjo

{"title":"First identification report for amino acid composition of red algae Gracilaria spp. obtained from Central Java, Indonesia.","authors":"Klara Kharisma Bunga Chandra, Tri Rini Nuringtyas, Tri Joko Raharjo","doi":"10.5114/bta.2024.145254","DOIUrl":"10.5114/bta.2024.145254","url":null,"abstract":"Gracilaria is a genus of red algae found mainly in Asia. Various species of Gracilaria are distributed throughout Indonesia's marine waters, especially in coastal areas, and are cultivated for research and economic purposes. Gracilaria mainly consists of polysaccharides and pigments, which have hindered protein identification. The unique protein expressions have never been analyzed using a proteomic approach, and no reports are available on Gracilaria spp. amino acid sequences in Central Java. Based on this background, we aimed to explore Gracilaria protein characterization using unique peptide sequence analysis via LC-HRMS. The usage of liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) has been growing in biomedical and environmental sciences, offering high accuracy in protein detection. We followed the LC-HRMS standard protocol with an optimized precipitation procedure. TCA/acetone precipitation was used for protein purification, after which the precipitate was subjected to protein digestion to obtain small peptide fractions. Protein analysis results are presented as protein concentrations, molecular models, and peptide sequences. This experiment identified four sequences derived from the Rhodophyta database: TKKILDK (845.5455 Da), TVKSLLTK (889.5717 Da), ILVKTLK (814.5761 Da), and TGcGRSKR (921.4683 Da). This study reveals peptide sequences for Gracilaria spp., showing similarities with other red algae species, along with the functions of the peptide sequences. Furthermore, amino acid models of secondary structures were provided to support our findings.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"325-335"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Newly developed genomic SSR markers revealed the population structure and genetic characteristics of abaca (Musa textilis Nee). 新开发的基因组SSR标记揭示了abaca （Musa textilis Nee）的群体结构和遗传特征。

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145255

Mariecris Rizalyn R Mendoza, Antonio C Laurena, Maria Genaleen Q Diaz, Eureka Teresa M Ocampo, Tonette P Laude, Antonio G Lalusin

{"title":"Newly developed genomic SSR markers revealed the population structure and genetic characteristics of abaca (Musa textilis Nee).","authors":"Mariecris Rizalyn R Mendoza, Antonio C Laurena, Maria Genaleen Q Diaz, Eureka Teresa M Ocampo, Tonette P Laude, Antonio G Lalusin","doi":"10.5114/bta.2024.145255","DOIUrl":"10.5114/bta.2024.145255","url":null,"abstract":"Abaca (Musa textilis Nee) is the primary source of manila hemp fiber, a vital industrial product for the country. Previous studies have relied on molecular markers designed for other Musa species or distant genera like rice, limiting accurate genetic characterization and germplasm conservation. To address this, we developed 50 genome-specific molecular markers based on the recently released whole genome sequence assembly of Abaca var. Abuab by Galvez et al. (2021). Among these markers, 28 showed high polymorphism, with an average PIC value of 0.78. Population analysis revealed a heterozygosity of 0.428, indicating moderate genetic diversity, supported by an alpha value of 0.0735 and an F stp value of 0.0815, which suggests moderate genetic differentiation among abaca accessions. Cluster analyses, generated by DARwin and STRUCTURE software with 91% similarity, identified four clusters. The new markers were also able to distinguish six Musa accessions exhibiting morphological traits of both abaca and banana. Discrepancies in sample identification due to identical or inverted names were resolved using population structure analysis. Molecular variance analysis showed a 12% variance among the four abaca subpopulations and 88% within populations, suggesting recent divergence. Our study highlights the diversity, identity, and genetic variation within the abaca collection using accurate, robust, cost-effective, and computationally simple genome-specific markers. These markers are pivotal for genetic studies of abaca, including traitmarker mapping and the differentiation of accessions even in the juvenile stage, when phenotypic differences may be subtle.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"337-353"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of CNS depressant and muscle relaxant effects of the ethnomedicinal plant Macropanax dispermus on Swiss Albino mice and its effect against oxidative stress. 民族药用植物大戟对瑞士白化小鼠中枢神经系统抑制和肌肉松弛作用的研究及其对氧化应激的影响

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141804

Syeda Rubaiya Afrin, Mohammad Rashedul Islam, Md Ashraful Alam, Ummah Tasnim Nisat, Bakul Akter, Mohammed Kamrul Hossain

{"title":"Investigation of CNS depressant and muscle relaxant effects of the ethnomedicinal plant Macropanax dispermus on Swiss Albino mice and its effect against oxidative stress.","authors":"Syeda Rubaiya Afrin, Mohammad Rashedul Islam, Md Ashraful Alam, Ummah Tasnim Nisat, Bakul Akter, Mohammed Kamrul Hossain","doi":"10.5114/bta.2024.141804","DOIUrl":"https://doi.org/10.5114/bta.2024.141804","url":null,"abstract":"Background and objective: Since plant-based natural drugs are widely accepted in modern times and possess numerous pharmacological effects with an extensive therapeutic range, an ethnomedicinal plant native to Bangladesh was selected to investigate for investigation of its various pharmacological effects. Macropanax dispermus has been traditionally used and has demonstrated numerous pharmacological effects in preclinical investigations. Therefore, this research aimed to assess the central nervous system (CNS) depressant and antioxidant activities of the crude methanol extracts of the stem barks (MDMS), leaves (MDML), and their different fractions.Methods: The CNS depressant activity was assessed using the hole cross, rota-rod, and elevated plus maze tests on Swiss Albino mice, while the antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl free radical, hydrogen peroxide (H2O2) nonradical scavenging, and ferric reducing power assays.Results: The conducted assays successfully demonstrated that the chloroform fraction of MDML is a significantly (P < 0.001) effective CNS depressant, muscle relaxant, and anxiolytic agent with excellent antioxidative effects compared to standard and control. The aqueous fraction of MDML also acted as a significantly (P < 0.001) active CNS depressant and muscle relaxant, and it was a highly active ferric-reducing agent. All effects were dose and concentration-dependent.Conclusion: The presence of various phytochemicals might contribute to these activities. However, further research is suggested to isolate their active compounds and evaluate their mechanisms of action.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"249-261"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the new substitution glycine to alanine within the Kringle-2 domain of reteplase: a molecular dynamics study. 研究网状血小板酶 Kringle-2 结构域中甘氨酸到丙氨酸的新取代：分子动力学研究。

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141801

Kaveh Haji-Allahverdipoor, Habib Eslami, Koosha Rokhzadi, Mokhtar Jalali Javaran, Sajad Rashidi Monfared, Mohamad Bagher Khademerfan

{"title":"Investigation of the new substitution glycine to alanine within the Kringle-2 domain of reteplase: a molecular dynamics study.","authors":"Kaveh Haji-Allahverdipoor, Habib Eslami, Koosha Rokhzadi, Mokhtar Jalali Javaran, Sajad Rashidi Monfared, Mohamad Bagher Khademerfan","doi":"10.5114/bta.2024.141801","DOIUrl":"https://doi.org/10.5114/bta.2024.141801","url":null,"abstract":"Background: Recombinant plasminogen activator (r-PA) consists of the Kringle-2 and protease domains of human tissue-type plasminogen. It is used clinically to treat coronary artery thrombosis and acute myocardial infarction. However, the expression and production of reteplase (r-PA) are limited due to its susceptibility to proteolysis during manufacturing processes. Therefore, efforts have been made to address this limitation.Materials and methods: To enhance the conformational stability of r-PA and increase its resistance to proteolysis, we used Gly 6 Ala substitutions in the Kringle-2 domain through in silico . We created an in silico mutant collection with eight structures, incorporating four designated mutations (R103S, G39A, G53A, and G55A). Using MODELLER software and homology modeling, we developed three-dimensional structures for two Kringle-2 and tissue plasminogen activator protease domains, including the wild noncleavable form (R103S) and mutants with all four designated mutations. We assessed protein stability using a dynamic cross-correlation matrix by extracting global properties such as Root Mean Square Deviation (RMSD) and Root Mean Square Fluctuation (RMSF) from trajectory files.Results: The findings revealed that a single glycine-alanine substitution (G39A) enhanced the conformational stability of r-PA, as evidenced by improvements in RMSD, RMSF, radius of gyration, surface accessibility, hydrogen bond formation, eigenvector projection, and density analysis.Conclusion: The conformational stability of r-PA conferred by glycine replacement with alanine may decrease the propensity for proteolysis in protease - rich environments across various recombinant systems and potentially enhance its production and expression levels.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"201-213"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanotechnology in cancer therapeutics, diagnosis, and management. 癌症治疗、诊断和管理中的纳米技术。

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141807

Disha Hazarika, Sumit Sarma, Priyanka Shankarishan

{"title":"Nanotechnology in cancer therapeutics, diagnosis, and management.","authors":"Disha Hazarika, Sumit Sarma, Priyanka Shankarishan","doi":"10.5114/bta.2024.141807","DOIUrl":"https://doi.org/10.5114/bta.2024.141807","url":null,"abstract":"Nanotechnology presents an exciting opportunity in cancer research by offering significant advancements in therapies, diagnosis, and management. It possesses unparalleled potential to enhance the accuracy and effectiveness of cancer therapy while simultaneously reducing adverse effects, owing to its distinctive capability to manipulate matter at a molecular level. Using nanoparticle carriers has facilitated the precise administration of therapeutic agents to afflicted areas within the human body through customized drug delivery systems, resulting in improved treatment accuracy and efficacy while reducing adverse effects. These techniques improve drug solubility and stability, leading to elevated levels of biochemical availability and improved efficacy outcomes for patients with minimal negative effects during treatment cycles. Another use case for nanoparticles includes tumor imaging; functionalized with targeting ligands containing diagnostic agents, they foster early detection, making quicker remedial action plans possible. Overall, the incorporation of nanotechnology ensures a promising future, although it stresses the need to address regulatory hurdles and safety concerns before widespread clinical implementation. Despite the complexity of cancer research and patient care, nanotechnology shows promise in transforming both fields.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"287-303"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of an immune response by a nonreplicating adenoviruses-based formulation versus a commercial pseudo-SARS-CoV-2 vaccine. 以非复制腺病毒为基础的制剂与商用伪 SARS-CoV-2 疫苗相比可诱导免疫反应。

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141805

Joanna Baran, Łukasz Kuryk, Mariangela Garofalo, Katarzyna Pancer, Magdalena Wieczorek, Michalina Kazek, Monika Staniszewska

{"title":"Induction of an immune response by a nonreplicating adenoviruses-based formulation versus a commercial pseudo-SARS-CoV-2 vaccine.","authors":"Joanna Baran, Łukasz Kuryk, Mariangela Garofalo, Katarzyna Pancer, Magdalena Wieczorek, Michalina Kazek, Monika Staniszewska","doi":"10.5114/bta.2024.141805","DOIUrl":"https://doi.org/10.5114/bta.2024.141805","url":null,"abstract":"Screening for effective vaccines requires broad studies on their immunogenicity in vitro and ex vivo . We used a PBMC-based system to assess changes in CD4+ T cells, CD8+ T cells, and CD19+ B cells upon stimulation with different combinations of antigens and adjuvants. We studied the activation mechanism using flow cytometry and two different adenoviral adjuvants characterized by the presence or absence of costimulatory ligands for the ICOS and CD40 receptors. Our studies identified the cellular targets and molecular mechanisms driving ongoing switched-antibody diversification. Class-switched memory B cells were the main precursor cells (95.03% ± 0.38 vs. mock 82.33% ± 0.45, P < 0.05) after treatment with the immunogenic formula: adenovirus armed (MIX1) or not (MIX2) with the ICOS and CD40 ligand, the recombinant receptor binding domain (rRBD), and Lentifect™ SARS-CoV-2 spike-pseudotyped lentivirus (GeneCopoeia, USA). Bcell class-switching towards the IgG+IgM+- positive phenotypes was noted (~50-fold increase vs. mock, P < 0.05). A significant increase was observed in the CD8+TEM population of the MIX1 (~2-fold, P < 0.05) and MIX2 (~4.7-fold, P < 0.05) treated samples. CD8+TEMRA increased when PBMCs were treated with MIX2 (9.63% ± 0.90, P < 0.05) vs. mock (2.63% ± 1.96). Class-switched memory B cells were the dominant antigen-specific cells in primary reactions. We indicated a correlation between the protection offered by vaccine regimens and their ability to induce high frequencies of multifunctional T cells.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"263-272"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of some explants for callus induction and plantlet regeneration in Solanum lycopersicum L. under treatment of different plant growth regulators. 在不同植物生长调节剂的作用下，某些外植体诱导番茄茄属植物胼胝体和小植株再生的潜力

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141803

Anjana Kumari, Avinash K Nagpal, Jatinder K Katnoria

{"title":"Potential of some explants for callus induction and plantlet regeneration in Solanum lycopersicum L. under treatment of different plant growth regulators.","authors":"Anjana Kumari, Avinash K Nagpal, Jatinder K Katnoria","doi":"10.5114/bta.2024.141803","DOIUrl":"https://doi.org/10.5114/bta.2024.141803","url":null,"abstract":"Plant growth regulators (PGRs) control signaling networks and developmental processes involved in plant responses to various biotic and abiotic stresses, making it crucial to study PGRs in vitro. The protocol for micropropagation of Solanum lycopersicum L., following callus induction and regeneration through explants such as internodal segments, leaves, and nodal segments, was established during the present study. Explants were inoculated on Murashige and Skoog (MS) medium supplemented with different plant growth regulators like BA (6-benzylaminopurine), 2,4-D (2,4-dichlorophenoxyacetic acid), BA + 2,4-D, IAA (Indoleacetic acid), IBA (Indolebutyric acid), and NAA (Naphthaleneacetic acid). It was observed that among all explants, the nodal segment showed maximum callus induction (83.33%) and multiplication (86.67%) at 0.25 mg/l of 2,4-D; the highest shoot number (3.33) at 0.5 mg/l of IAA + 0.5 mg/l of BA; the greatest shoot length (7.57 cm) at 0.75 mg/l of BA; root induction (80.95%), root number (21.88), and root length (10.62 cm) at 1 mg/l of IAA. Additionally, the maximum fresh weight (2.448 g) was observed at 0.5 mg/l of BA, while the highest dry weight (0.172 g) and dry matter content (14.25%) were noted at 1 mg/l of BA + 1 mg/l of 2,4-D for the internodal segment. Results of the present study revealed that among different explants, the best response was given by nodal segments, followed by internodal segments. Among the different PGRs, 2,4-D resulted in the highest callus induction and multiplication percentage.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"227-247"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0