Biotechnologia最新文献_第2页

Impact of salinity on the morpho-biochemical traits of hydroponically cultivated Spinacia oleracea L. 盐度对水培菠菜形态生化性状的影响。

Biotechnologia Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.5114/bta/202318

Archana A Naik, Shekhar D Tidke, Mahadev R Chambhare, Ravindra D Bansode, Panchshila S Kabnoorkar

{"title":"Impact of salinity on the morpho-biochemical traits of hydroponically cultivated Spinacia oleracea L.","authors":"Archana A Naik, Shekhar D Tidke, Mahadev R Chambhare, Ravindra D Bansode, Panchshila S Kabnoorkar","doi":"10.5114/bta/202318","DOIUrl":"10.5114/bta/202318","url":null,"abstract":"Background: Spinacia oleracea, a nutrient-dense vegetable composed of 91% water, 4% starch, and 3% protein, is a staple in the Indian diet. However, salinity stress can hinder its growth. This study examines the effects of salinity on the morpho-biochemical traits of spinach in a hydroponic system.Materials and methods: Spinach seeds were sown on coco peat, and after 10 days, the seedlings were transferred to the hydroponic setup. The plants were treated with salt concentrations of ECe 0, 4.0, 6.0, 8.0, 10.0, and 12.0 ds/m, and various morpho-biochemical parameters were assessed at 10-day intervals.Results: In the control group, seed germination was 59.6 ± 1.45%, while increasing salt concentrations (4 to 10 dS/m) progressively reduced germination (50 ± 1.2% to 14 ± 2%). Root and shoot lengths (root: 14.16 ± 0.19 cm; shoot: 4.23 ± 0.28 cm at 4 dS/m), relative water content (47.5 ± 0.43% to 32.1 ± 0.86%), and leaf surface area (25.03 ± 0.26 to 9 ± 0.12 cm2) all declined with increasing salinity. Conversely, proline content (0.055 ± 0 to 0.12 ± 0 μg/ml) and antioxidant enzyme activity (SOD: 1.83±0.04/g protein; APX: 0.53 ± 0.1/mg protein; CAT: 0.0054 ± 0/mg protein) increased compared to the control. However, chlorophyll content (3.73 ±0.02 to 1.95 ± 0.03 mg/g at 12 dS/m) and protein content (0.13 ± 0 at 4 dS/m to 0.054 ± 0 μg/ml at 12 dS/m) decreased with rising salinity.Conclusions: Therefore, it is concluded that spinach grown hydroponically can tolerate salt stress up to ECe 6.0 dS/m after 30 days of treatments, and more increased (8.0 to 12 dS/m) salt concentration that adversely affects overall morpho-biochemical performance.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 1","pages":"49-62"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expectations and limitations of biotechnology in the perspective of 2050. 展望2050年生物技术的发展前景与局限性。

Biotechnologia Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.5114/bta/203189

Tomasz Twardowski

引用次数: 0

Evaluation of the physical, chemical, antioxidant, and antibacterial properties of Camellia oleifera Abel. seed oil. 油茶的物理、化学、抗氧化和抗菌性能评价。籽油。

Biotechnologia Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.5114/bta/195496

Pham My Hao, Luu Thao Nguyen, Tran Thi Mai Anh, Le Pham Tan Quoc

{"title":"Evaluation of the physical, chemical, antioxidant, and antibacterial properties of Camellia oleifera Abel. seed oil.","authors":"Pham My Hao, Luu Thao Nguyen, Tran Thi Mai Anh, Le Pham Tan Quoc","doi":"10.5114/bta/195496","DOIUrl":"10.5114/bta/195496","url":null,"abstract":"Background: Camellia seeds are rich in oil and contain fatty acids that offer significant health benefits.Materials and methods: This study aimed to characterize Camellia oleifera seeds and their oil. Physical properties of the seeds, including 1000-seed weight, density, moisture content, specific gravity, and angle of repose, were determined. Camellia seed oil was extracted using the pressing method, and various physicochemical and biological properties of the oil-such as density, color, acid, peroxide, and saponification values, as well as antioxidant and antibacterial activities-were assessed.Results: Pressing the oil at 140oC yielded optimal results, achieving a recovery efficiency of 21.67%. Gas chromatography-mass spectrometry identified seven fatty acid components in the oil, with oleic acid (71.03%) being the most abundant. The antioxidant capacity of the oil was evaluated using a DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, yielding an IC50 value of 265.8 mg/mL. However, the oil exhibited no inhibitory effects against four bacterial strains (Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, and Staphylococcus aureus).Conclusions: These findings highlight the potential of Camellia seed oil in food technology, particularly as an alternative to commercial cooking oils.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 1","pages":"5-12"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico screening and identification of CTL and HTL epitopes in the secreted virulence factors of Mycobacterium tuberculosis. 结核分枝杆菌分泌毒力因子中CTL和HTL表位的筛选与鉴定。

Biotechnologia Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.5114/bta/201461

Edward Kevin B Bragais, Francisco M Heralde, Kim Claudette J Fernandez, Salvador Eugenio C Caoili, Leana Rich Herrera-Ong

{"title":"In silico screening and identification of CTL and HTL epitopes in the secreted virulence factors of Mycobacterium tuberculosis.","authors":"Edward Kevin B Bragais, Francisco M Heralde, Kim Claudette J Fernandez, Salvador Eugenio C Caoili, Leana Rich Herrera-Ong","doi":"10.5114/bta/201461","DOIUrl":"10.5114/bta/201461","url":null,"abstract":"Background: Mycobacterium tuberculosis (MTb) is a highly infectious pathogen and a global health threat due to its resilient cell wall and immune evasion strategies. Despite the availability of the antituberculosis Bacille Calmette-Guérin (BCG) vaccine, its efficacy varies (0%-80%) and gradually decreases over time. This study aimed to identify cytotoxic T-lymphocyte (CTL) and helper T-lymphocyte (HTL) epitopes in MTb secretory proteins using immunoinformatics tools.Materials and methods: The Protein Variability Server was used to identify highly conserved sequences, and epitope population coverage was estimated for the Southeast Asian (SEA) region. Selected epitopes were also docked to their major histocompatibility complex alleles.Results: Five secretory proteins critical to MTb pathogenesis and virulence were identified as antigenic (antigenicity score > 0.4). Predicted epitopes had IC50 values ≤ 500 nM, indicating strong binding affinity, with an estimated 94% population coverage in SEA. All candidate epitopes were highly conserved (Shannon index ≤ 0.1) and showed no significant sequence similarity to human proteins, allergens, or toxic peptides. Docking analysis confirmed favorable binding to their corresponding HLA alleles, as indicated by low Gibbs free energy change (ΔG) values and dissociation constants (K D nM).Conclusions: Overall, this study identified immunoactive CTL and HTL epitopes that could serve as promising candidates for future antiTB vaccine development. Further in vitro and in vivo studies are required to validate these preliminary findings.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"106 1","pages":"63-76"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutting-edge AI tools revolutionizing scientific research in life sciences. 尖端的人工智能工具革新了生命科学的科学研究。

Biotechnologia Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.5114/bta/200803

Katarzyna Lorenc-Kukuła

引用次数: 0

In silico evaluation, characterization, and in vitro anticancer activity of curcumin-nimbin loaded nanoformulation in HCT-116 cell lines. 姜黄素-宁宾负载纳米制剂在HCT-116细胞系中的体外抗癌活性

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145256

Arumugam Madeswaran, Selvam Tamilazhagan, Sellappan Mohan

{"title":"In silico evaluation, characterization, and in vitro anticancer activity of curcumin-nimbin loaded nanoformulation in HCT-116 cell lines.","authors":"Arumugam Madeswaran, Selvam Tamilazhagan, Sellappan Mohan","doi":"10.5114/bta.2024.145256","DOIUrl":"10.5114/bta.2024.145256","url":null,"abstract":"Colorectal cancer is one of the most prevalent malignancies worldwide and a leading cause of mortality. Chemotherapy medications are often limited in use due to issues like drug resistance, P-glycoprotein efflux, and relapse of chemotherapy. In this study, we formulated a nanosuspension with curcumin and nimbin to address these limitations and assessed its anticancer potential using in silico molecular docking and in vitro MTT assay.Methods: In silico docking and ADMET analyses targeted proteins implicated in colorectal cancer, with doxorubicin as the standard. The docking studies were conducted using AutoDock 4.2, while in vitro anticancer activity was assessed through the MTT assay in HCT 116 cell lines.Results: In silico docking of curcumin and nimbin showed significant interactions with target proteins compared to the standard. ADMET analysis indicated favorable Caco-2 permeability and intestinal absorption of the selected phytoconstituents. The MTT assay demonstrated concentration-dependent cell viability inhibition in HCT 116 cell lines treated with the nanosuspension, with an IC50 value of 30%.Conclusion: The curcumin-nimbin loaded nanosuspension demonstrated promising anticancer activity against HCT 116 cell lines in both in silico and in vitro studies. Further studies are required to evaluate the anticancer effect of curcumin-nimbin loaded nanosupension through clinical and preclinical studies for the progress of potential formulation in the treatment of colorectal cancer.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"355-365"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm architecture and dynamics of the oral ecosystem. 口腔生态系统的生物膜结构与动力学。

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145259

Rina Rani Ray

{"title":"Biofilm architecture and dynamics of the oral ecosystem.","authors":"Rina Rani Ray","doi":"10.5114/bta.2024.145259","DOIUrl":"10.5114/bta.2024.145259","url":null,"abstract":"The oral cavity, being a nutritionally enriched environment, has been proven to be an ideal habitat for biofilm development. Various microenvironments, including dental enamel, supra- and subgingival surfaces, salivary fluid, and the dorsal surface of the tongue, harbor diverse microbes. These biofilms typically consist of four major layers. Depending on the food, age, clinical state, and lifestyle of the patient, the microbial growth dynamics in oral biofilm varies significantly. The presence of pathogenic bacteria that disrupt the normal floral composition of the oral cavity can lead to plaque biofilm formation, which is a precursor to various diseases. Noteworthy pathogenic bacteria, such as Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans, often initiate biofilm formation. Undiagnosed and untreated oral biofilm can lead to severe diseases like periodontitis and eventual tooth loss. Therefore, studying the architecture and dynamics of oral biofilms is essential and can be achieved through image analysis and modern technologies, such as AI-enabled technologies and surface topography-adaptive robotic superstructures.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"395-402"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gliclazide protects ionizing radiation-induced intestinal injury in mice by inhibiting oxidative stress and caspase-3. 格列齐特通过抑制氧化应激和caspase-3来保护电离辐射诱导的小鼠肠道损伤。

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145257

Soroush Arzani, Soghra Farzipour, Fereshteh Talebpour Amiri, Seyed Jalal Hosseinimehr

{"title":"Gliclazide protects ionizing radiation-induced intestinal injury in mice by inhibiting oxidative stress and caspase-3.","authors":"Soroush Arzani, Soghra Farzipour, Fereshteh Talebpour Amiri, Seyed Jalal Hosseinimehr","doi":"10.5114/bta.2024.145257","DOIUrl":"10.5114/bta.2024.145257","url":null,"abstract":"Gliclazide (GLZ), an oral antihyperglycemic medication, has additional beneficial effects, such as anti-inflammatory and antioxidant properties, besides lowering blood glucose levels. In this study, the radio-protective effect of GLZ was evaluated against ionizing radiation (IR)-induced intestinal injury in mice. Eight groups of mice were randomized as follows: control, GLZ (5, 10, and 25 mg/kg), IR (6 Gy), and IR + GLZ (at 5, 10, and 25 mg/kg). GLZ was administered to the mice for eight consecutive days, after which they were exposed to X-rays at a single dose of 6 Gy. After irradiation, biochemical parameters, immunohistochemical, and histological examinations were conducted on the ileum of the mice. IR exposure increased the levels of malondialdehyde and protein carbonyl, while glutathione levels, as oxidative stress biomarkers, decreased. Apoptosis in ileum tissues was also assessed. Furthermore, histopathological changes were observed in the irradiated mice. GLZ treatment significantly mitigated these changes. The administration of GLZ resulted in a marked decrease in caspase-3 immunoreactivity in the ileum of irradiated mice. This preclinical study exhibited that GLZ has a radioprotective effect against intestinal injury by inhibiting oxidative stress and apoptosis.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"367-376"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin nanoparticles as a therapeutic approach: pharmacological actions and potential applications in therapy. 槲皮素纳米颗粒作为一种治疗方法：药理作用和潜在的治疗应用。

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145258

Reham Farouk El-Sayed Baiomy

{"title":"Quercetin nanoparticles as a therapeutic approach: pharmacological actions and potential applications in therapy.","authors":"Reham Farouk El-Sayed Baiomy","doi":"10.5114/bta.2024.145258","DOIUrl":"10.5114/bta.2024.145258","url":null,"abstract":"The utilization of quercetin nanoparticles as a novel therapeutic strategy has garnered significant attention in recent years. These nanoparticles offer a unique approach to enhancing delivery and effectiveness while overcoming the drawbacks of quercetin. By exploiting the advantages of nanotechnology, such as increased stability and improved bioavailability, quercetin nanoparticles hold significant potential for developing innovative treatments across various medical fields. Quercetin nanoparticles have emerged as an indispensable component in numerous pharmaceutical and medicinal formulations. They are recognized for their anticancer, antitumor, anti-inflammatory, and antidiabetic properties, making them valuable in addressing allergic reactions, metabolic disorders, inflammatory disorders, cardiovascular diseases, and arthritis. From a pharmacological perspective, quercetin nanoparticles have demonstrated beneficial effects against Alzheimer's disease, primarily through their inhibitory impact on acetylcholinesterase. Furthermore, these nanoparticles have been scientifically documented to possess antioxidant, anticarcinogenic, hepatoprotective, and cytotoxic activities. This comprehensive review aims to explore the pharmacokinetics and biological activities associated with quercetin nanoparticles. It also highlights their potential as therapeutic agents in treating a wide range of diseases, including Alzheimer's disease, cancer, and neurodegenerative disorders.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"377-393"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of antioxidant, antimicrobial, and enzymatic properties of thermophilic cyanobacteria extracts. 嗜热蓝藻提取物的抗氧化、抗菌和酶促特性研究。

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145253

Aytan Fataliyeva, Meral Yilmaz Cankilic, Nalan Yilmaz Sariozlu

{"title":"Investigation of antioxidant, antimicrobial, and enzymatic properties of thermophilic cyanobacteria extracts.","authors":"Aytan Fataliyeva, Meral Yilmaz Cankilic, Nalan Yilmaz Sariozlu","doi":"10.5114/bta.2024.145253","DOIUrl":"10.5114/bta.2024.145253","url":null,"abstract":"Background: The present study investigated the antioxidant, antimicrobial, and partial enzymatic properties of 52 thermophilic cyanobacteria isolates in vitro.Materials and methods: The DPPH scavenging method was applied to test the antioxidant potential of isolates' methanol extracts. Agar block diffusion and agar well diffusion methods were used to evaluate the antimicrobial activity and measured in milimeters. The measurement of enzyme activity was carried out by a modification of the agar block method by the growth of the cyanobacteria.Results: Among the cyanobacterial extracts, strain 37 (0.78 ± 0.055 mg/ml) showed an IC50 value close to ascorbic acid (0.22 ± 0.04 mg/ml), indicating that it has a specific antioxidant source. Isolate G13 was shown to have the strongest antimicrobial activity against Micrococcus luteus NRRL B-4375 in the agar well diffusion method. In addition, the ability to produce enzymes was determined in isolate G1 (25 ± 5.66 mm), which had the highest cellulase activity at pH 8, and isolate K42 (22 ± 0.71 mm), which had the highest lipase activity at pH 7.Conclusion: When percent inhibition and IC50 values were examined, it was found that cyanobacterial methanol extracts had moderate and low scavenging activity in comparison to the standard antioxidant ascorbic acid. In a study using the agar well diffusion method, the activity of cyanobacterial extracts against test bacteria was observed. In light of the results obtained, it is believed that the isolates exhibited lipase and cellulase (pH 7 and 8) enzyme activity at both pH levels and have potential for industrial use.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"311-323"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0