Biotechnologia最新文献_第3页

First identification report for amino acid composition of red algae Gracilaria spp. obtained from Central Java, Indonesia. 印度尼西亚中爪哇红藻紫菜属（Gracilaria spp.）氨基酸组成首次鉴定报告。

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145254

Klara Kharisma Bunga Chandra, Tri Rini Nuringtyas, Tri Joko Raharjo

{"title":"First identification report for amino acid composition of red algae Gracilaria spp. obtained from Central Java, Indonesia.","authors":"Klara Kharisma Bunga Chandra, Tri Rini Nuringtyas, Tri Joko Raharjo","doi":"10.5114/bta.2024.145254","DOIUrl":"10.5114/bta.2024.145254","url":null,"abstract":"Gracilaria is a genus of red algae found mainly in Asia. Various species of Gracilaria are distributed throughout Indonesia's marine waters, especially in coastal areas, and are cultivated for research and economic purposes. Gracilaria mainly consists of polysaccharides and pigments, which have hindered protein identification. The unique protein expressions have never been analyzed using a proteomic approach, and no reports are available on Gracilaria spp. amino acid sequences in Central Java. Based on this background, we aimed to explore Gracilaria protein characterization using unique peptide sequence analysis via LC-HRMS. The usage of liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) has been growing in biomedical and environmental sciences, offering high accuracy in protein detection. We followed the LC-HRMS standard protocol with an optimized precipitation procedure. TCA/acetone precipitation was used for protein purification, after which the precipitate was subjected to protein digestion to obtain small peptide fractions. Protein analysis results are presented as protein concentrations, molecular models, and peptide sequences. This experiment identified four sequences derived from the Rhodophyta database: TKKILDK (845.5455 Da), TVKSLLTK (889.5717 Da), ILVKTLK (814.5761 Da), and TGcGRSKR (921.4683 Da). This study reveals peptide sequences for Gracilaria spp., showing similarities with other red algae species, along with the functions of the peptide sequences. Furthermore, amino acid models of secondary structures were provided to support our findings.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"325-335"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Newly developed genomic SSR markers revealed the population structure and genetic characteristics of abaca (Musa textilis Nee). 新开发的基因组SSR标记揭示了abaca （Musa textilis Nee）的群体结构和遗传特征。

Biotechnologia Pub Date : 2024-12-19 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.145255

Mariecris Rizalyn R Mendoza, Antonio C Laurena, Maria Genaleen Q Diaz, Eureka Teresa M Ocampo, Tonette P Laude, Antonio G Lalusin

{"title":"Newly developed genomic SSR markers revealed the population structure and genetic characteristics of abaca (Musa textilis Nee).","authors":"Mariecris Rizalyn R Mendoza, Antonio C Laurena, Maria Genaleen Q Diaz, Eureka Teresa M Ocampo, Tonette P Laude, Antonio G Lalusin","doi":"10.5114/bta.2024.145255","DOIUrl":"10.5114/bta.2024.145255","url":null,"abstract":"Abaca (Musa textilis Nee) is the primary source of manila hemp fiber, a vital industrial product for the country. Previous studies have relied on molecular markers designed for other Musa species or distant genera like rice, limiting accurate genetic characterization and germplasm conservation. To address this, we developed 50 genome-specific molecular markers based on the recently released whole genome sequence assembly of Abaca var. Abuab by Galvez et al. (2021). Among these markers, 28 showed high polymorphism, with an average PIC value of 0.78. Population analysis revealed a heterozygosity of 0.428, indicating moderate genetic diversity, supported by an alpha value of 0.0735 and an F stp value of 0.0815, which suggests moderate genetic differentiation among abaca accessions. Cluster analyses, generated by DARwin and STRUCTURE software with 91% similarity, identified four clusters. The new markers were also able to distinguish six Musa accessions exhibiting morphological traits of both abaca and banana. Discrepancies in sample identification due to identical or inverted names were resolved using population structure analysis. Molecular variance analysis showed a 12% variance among the four abaca subpopulations and 88% within populations, suggesting recent divergence. Our study highlights the diversity, identity, and genetic variation within the abaca collection using accurate, robust, cost-effective, and computationally simple genome-specific markers. These markers are pivotal for genetic studies of abaca, including traitmarker mapping and the differentiation of accessions even in the juvenile stage, when phenotypic differences may be subtle.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 4","pages":"337-353"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of CNS depressant and muscle relaxant effects of the ethnomedicinal plant Macropanax dispermus on Swiss Albino mice and its effect against oxidative stress. 民族药用植物大戟对瑞士白化小鼠中枢神经系统抑制和肌肉松弛作用的研究及其对氧化应激的影响

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141804

Syeda Rubaiya Afrin, Mohammad Rashedul Islam, Md Ashraful Alam, Ummah Tasnim Nisat, Bakul Akter, Mohammed Kamrul Hossain

{"title":"Investigation of CNS depressant and muscle relaxant effects of the ethnomedicinal plant Macropanax dispermus on Swiss Albino mice and its effect against oxidative stress.","authors":"Syeda Rubaiya Afrin, Mohammad Rashedul Islam, Md Ashraful Alam, Ummah Tasnim Nisat, Bakul Akter, Mohammed Kamrul Hossain","doi":"10.5114/bta.2024.141804","DOIUrl":"https://doi.org/10.5114/bta.2024.141804","url":null,"abstract":"Background and objective: Since plant-based natural drugs are widely accepted in modern times and possess numerous pharmacological effects with an extensive therapeutic range, an ethnomedicinal plant native to Bangladesh was selected to investigate for investigation of its various pharmacological effects. Macropanax dispermus has been traditionally used and has demonstrated numerous pharmacological effects in preclinical investigations. Therefore, this research aimed to assess the central nervous system (CNS) depressant and antioxidant activities of the crude methanol extracts of the stem barks (MDMS), leaves (MDML), and their different fractions.Methods: The CNS depressant activity was assessed using the hole cross, rota-rod, and elevated plus maze tests on Swiss Albino mice, while the antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl free radical, hydrogen peroxide (H2O2) nonradical scavenging, and ferric reducing power assays.Results: The conducted assays successfully demonstrated that the chloroform fraction of MDML is a significantly (P < 0.001) effective CNS depressant, muscle relaxant, and anxiolytic agent with excellent antioxidative effects compared to standard and control. The aqueous fraction of MDML also acted as a significantly (P < 0.001) active CNS depressant and muscle relaxant, and it was a highly active ferric-reducing agent. All effects were dose and concentration-dependent.Conclusion: The presence of various phytochemicals might contribute to these activities. However, further research is suggested to isolate their active compounds and evaluate their mechanisms of action.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"249-261"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the new substitution glycine to alanine within the Kringle-2 domain of reteplase: a molecular dynamics study. 研究网状血小板酶 Kringle-2 结构域中甘氨酸到丙氨酸的新取代：分子动力学研究。

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141801

Kaveh Haji-Allahverdipoor, Habib Eslami, Koosha Rokhzadi, Mokhtar Jalali Javaran, Sajad Rashidi Monfared, Mohamad Bagher Khademerfan

{"title":"Investigation of the new substitution glycine to alanine within the Kringle-2 domain of reteplase: a molecular dynamics study.","authors":"Kaveh Haji-Allahverdipoor, Habib Eslami, Koosha Rokhzadi, Mokhtar Jalali Javaran, Sajad Rashidi Monfared, Mohamad Bagher Khademerfan","doi":"10.5114/bta.2024.141801","DOIUrl":"https://doi.org/10.5114/bta.2024.141801","url":null,"abstract":"Background: Recombinant plasminogen activator (r-PA) consists of the Kringle-2 and protease domains of human tissue-type plasminogen. It is used clinically to treat coronary artery thrombosis and acute myocardial infarction. However, the expression and production of reteplase (r-PA) are limited due to its susceptibility to proteolysis during manufacturing processes. Therefore, efforts have been made to address this limitation.Materials and methods: To enhance the conformational stability of r-PA and increase its resistance to proteolysis, we used Gly 6 Ala substitutions in the Kringle-2 domain through in silico . We created an in silico mutant collection with eight structures, incorporating four designated mutations (R103S, G39A, G53A, and G55A). Using MODELLER software and homology modeling, we developed three-dimensional structures for two Kringle-2 and tissue plasminogen activator protease domains, including the wild noncleavable form (R103S) and mutants with all four designated mutations. We assessed protein stability using a dynamic cross-correlation matrix by extracting global properties such as Root Mean Square Deviation (RMSD) and Root Mean Square Fluctuation (RMSF) from trajectory files.Results: The findings revealed that a single glycine-alanine substitution (G39A) enhanced the conformational stability of r-PA, as evidenced by improvements in RMSD, RMSF, radius of gyration, surface accessibility, hydrogen bond formation, eigenvector projection, and density analysis.Conclusion: The conformational stability of r-PA conferred by glycine replacement with alanine may decrease the propensity for proteolysis in protease - rich environments across various recombinant systems and potentially enhance its production and expression levels.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"201-213"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanotechnology in cancer therapeutics, diagnosis, and management. 癌症治疗、诊断和管理中的纳米技术。

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141807

Disha Hazarika, Sumit Sarma, Priyanka Shankarishan

{"title":"Nanotechnology in cancer therapeutics, diagnosis, and management.","authors":"Disha Hazarika, Sumit Sarma, Priyanka Shankarishan","doi":"10.5114/bta.2024.141807","DOIUrl":"https://doi.org/10.5114/bta.2024.141807","url":null,"abstract":"Nanotechnology presents an exciting opportunity in cancer research by offering significant advancements in therapies, diagnosis, and management. It possesses unparalleled potential to enhance the accuracy and effectiveness of cancer therapy while simultaneously reducing adverse effects, owing to its distinctive capability to manipulate matter at a molecular level. Using nanoparticle carriers has facilitated the precise administration of therapeutic agents to afflicted areas within the human body through customized drug delivery systems, resulting in improved treatment accuracy and efficacy while reducing adverse effects. These techniques improve drug solubility and stability, leading to elevated levels of biochemical availability and improved efficacy outcomes for patients with minimal negative effects during treatment cycles. Another use case for nanoparticles includes tumor imaging; functionalized with targeting ligands containing diagnostic agents, they foster early detection, making quicker remedial action plans possible. Overall, the incorporation of nanotechnology ensures a promising future, although it stresses the need to address regulatory hurdles and safety concerns before widespread clinical implementation. Despite the complexity of cancer research and patient care, nanotechnology shows promise in transforming both fields.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"287-303"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of an immune response by a nonreplicating adenoviruses-based formulation versus a commercial pseudo-SARS-CoV-2 vaccine. 以非复制腺病毒为基础的制剂与商用伪 SARS-CoV-2 疫苗相比可诱导免疫反应。

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141805

Joanna Baran, Łukasz Kuryk, Mariangela Garofalo, Katarzyna Pancer, Magdalena Wieczorek, Michalina Kazek, Monika Staniszewska

{"title":"Induction of an immune response by a nonreplicating adenoviruses-based formulation versus a commercial pseudo-SARS-CoV-2 vaccine.","authors":"Joanna Baran, Łukasz Kuryk, Mariangela Garofalo, Katarzyna Pancer, Magdalena Wieczorek, Michalina Kazek, Monika Staniszewska","doi":"10.5114/bta.2024.141805","DOIUrl":"https://doi.org/10.5114/bta.2024.141805","url":null,"abstract":"Screening for effective vaccines requires broad studies on their immunogenicity in vitro and ex vivo . We used a PBMC-based system to assess changes in CD4+ T cells, CD8+ T cells, and CD19+ B cells upon stimulation with different combinations of antigens and adjuvants. We studied the activation mechanism using flow cytometry and two different adenoviral adjuvants characterized by the presence or absence of costimulatory ligands for the ICOS and CD40 receptors. Our studies identified the cellular targets and molecular mechanisms driving ongoing switched-antibody diversification. Class-switched memory B cells were the main precursor cells (95.03% ± 0.38 vs. mock 82.33% ± 0.45, P < 0.05) after treatment with the immunogenic formula: adenovirus armed (MIX1) or not (MIX2) with the ICOS and CD40 ligand, the recombinant receptor binding domain (rRBD), and Lentifect™ SARS-CoV-2 spike-pseudotyped lentivirus (GeneCopoeia, USA). Bcell class-switching towards the IgG+IgM+- positive phenotypes was noted (~50-fold increase vs. mock, P < 0.05). A significant increase was observed in the CD8+TEM population of the MIX1 (~2-fold, P < 0.05) and MIX2 (~4.7-fold, P < 0.05) treated samples. CD8+TEMRA increased when PBMCs were treated with MIX2 (9.63% ± 0.90, P < 0.05) vs. mock (2.63% ± 1.96). Class-switched memory B cells were the dominant antigen-specific cells in primary reactions. We indicated a correlation between the protection offered by vaccine regimens and their ability to induce high frequencies of multifunctional T cells.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"263-272"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of some explants for callus induction and plantlet regeneration in Solanum lycopersicum L. under treatment of different plant growth regulators. 在不同植物生长调节剂的作用下，某些外植体诱导番茄茄属植物胼胝体和小植株再生的潜力

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141803

Anjana Kumari, Avinash K Nagpal, Jatinder K Katnoria

{"title":"Potential of some explants for callus induction and plantlet regeneration in Solanum lycopersicum L. under treatment of different plant growth regulators.","authors":"Anjana Kumari, Avinash K Nagpal, Jatinder K Katnoria","doi":"10.5114/bta.2024.141803","DOIUrl":"https://doi.org/10.5114/bta.2024.141803","url":null,"abstract":"Plant growth regulators (PGRs) control signaling networks and developmental processes involved in plant responses to various biotic and abiotic stresses, making it crucial to study PGRs in vitro. The protocol for micropropagation of Solanum lycopersicum L., following callus induction and regeneration through explants such as internodal segments, leaves, and nodal segments, was established during the present study. Explants were inoculated on Murashige and Skoog (MS) medium supplemented with different plant growth regulators like BA (6-benzylaminopurine), 2,4-D (2,4-dichlorophenoxyacetic acid), BA + 2,4-D, IAA (Indoleacetic acid), IBA (Indolebutyric acid), and NAA (Naphthaleneacetic acid). It was observed that among all explants, the nodal segment showed maximum callus induction (83.33%) and multiplication (86.67%) at 0.25 mg/l of 2,4-D; the highest shoot number (3.33) at 0.5 mg/l of IAA + 0.5 mg/l of BA; the greatest shoot length (7.57 cm) at 0.75 mg/l of BA; root induction (80.95%), root number (21.88), and root length (10.62 cm) at 1 mg/l of IAA. Additionally, the maximum fresh weight (2.448 g) was observed at 0.5 mg/l of BA, while the highest dry weight (0.172 g) and dry matter content (14.25%) were noted at 1 mg/l of BA + 1 mg/l of 2,4-D for the internodal segment. Results of the present study revealed that among different explants, the best response was given by nodal segments, followed by internodal segments. Among the different PGRs, 2,4-D resulted in the highest callus induction and multiplication percentage.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"227-247"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on xylanase sources, classification, mode of action, fermentation processes, and applications as a promising biocatalyst. 综述木聚糖酶的来源、分类、作用方式、发酵过程以及作为一种有前途的生物催化剂的应用。

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141806

Tariku Abena, Addis Simachew

{"title":"A review on xylanase sources, classification, mode of action, fermentation processes, and applications as a promising biocatalyst.","authors":"Tariku Abena, Addis Simachew","doi":"10.5114/bta.2024.141806","DOIUrl":"https://doi.org/10.5114/bta.2024.141806","url":null,"abstract":"The utilization of hydrolytic enzymes in various industrial processes worldwide has gained more attention than chemical catalysts due to the high selectivity of enzymes, their ease of control, and their negligible environmental impact, as they produce very small amounts of byproducts. Xylanase is one such enzyme that catalyzes the breakdown of the β-1,4 linkage of xylan, the second most abundant renewable heteropolysaccharide and hemicellulosic constituent of the plant cell wall. Naturally, xylanase can be obtained from various sources such as mollusks, insects, plants, animals, and various microorganisms (bacteria, fungi, yeast, and algae). The utilization of xylanase could greatly improve the overall economics of processing lignocellulosic materials for the generation of monosaccharides, liquid fuels, and chemicals. Microbial xylanase is suitable for applications in food and feed, paper and pulp, textile, pharmaceutical, and biorefining industries. It has gained global attention due to its substrate specificities, biochemical properties, and various biotechnological applications. This review focuses on xylanase production, sources, fermentation processes, modes of action, purification methods, and applications in various industries.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"273-285"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the antioxidant, antidiabetic, and antibacterial potential of postbiotic compounds derived from Lactiplantibacillus plantarum O7S1. 探索从植物乳杆菌 O7S1 中提取的后生物化合物的抗氧化、抗糖尿病和抗菌潜力。

Biotechnologia Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.141802

Nadia Aliouche, Mohamed Sifour, Houria Ouled-Haddar

{"title":"Exploring the antioxidant, antidiabetic, and antibacterial potential of postbiotic compounds derived from Lactiplantibacillus plantarum O7S1.","authors":"Nadia Aliouche, Mohamed Sifour, Houria Ouled-Haddar","doi":"10.5114/bta.2024.141802","DOIUrl":"https://doi.org/10.5114/bta.2024.141802","url":null,"abstract":"Probiotic bacteria are distinguished by their ability to produce various functional postbiotic metabolites. Therefore, this study aimed to explore the in vitro antioxidant, antidiabetic, and antibacterial properties of two postbiotics generated by Lactiplantibacillus plantarum O7S1 (Lpb. plantarum O7S1) during the fermentation process: cell-free supernatant (CFS) and exopolysaccharides (EPS). The antioxidant potential of these postbiotics was assessed using various radical scavenging assays and ferric-reducing antioxidant potential. The antidiabetic activity was evaluated through α-amylase inhibitory assays, while antibacterial activity was determined using agar well diffusion assays. The results of the present study revealed that CFS exhibited significant antioxidant and antidiabetic efficacy in contrast to EPS (P < 0.001). Specifically, CFS displayed remarkable scavenging ability against DPPH, hydroxyl, and superoxide radicals, with inhibition rates of 88.78, 78.91, and 34.85%, respectively, while EPS showed comparatively lower inhibition rates. Additionally, CFS demonstrated higher reducing activity (0.30 optical density units at 700 nm) and potent α-amylase inhibitory activity (95.87%) compared to EPS (67.17%) (P < 0.001). The agar well diffusion assay reported that CFS showed significant antimicrobial activity against both Gram-positive and Gram-negative pathogens, while no activity was observed with EPS. Furthermore, carbohydrate fermentation analysis indicated the strain's ability to metabolize various carbohydrates and their derivatives, potentially enhancing digestive health. These findings suggest that both CFS and EPS exhibit promising hypoglycemic, antioxidant, and antibacterial properties, making them potential candidates for incorporation into functional foods and pharmaceuticals aimed at preventing oxidative damage, diabetes, and pathogenic bacterial infections.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 3","pages":"215-225"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical composition, antioxidant, antibacterial, and enzyme inhibitory activities of organic extracts from flower buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry. Cleistocalyx operculatus (Roxb.) Merr. et Perry.

Biotechnologia Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.5114/bta.2024.139753

Doan Thien Thanh, Vo Kieu Oanh, Hoang Chinh Nguyen, Luong Thi My Ngan, Tran Trung Hieu

{"title":"Phytochemical composition, antioxidant, antibacterial, and enzyme inhibitory activities of organic extracts from flower buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry.","authors":"Doan Thien Thanh, Vo Kieu Oanh, Hoang Chinh Nguyen, Luong Thi My Ngan, Tran Trung Hieu","doi":"10.5114/bta.2024.139753","DOIUrl":"10.5114/bta.2024.139753","url":null,"abstract":"Cleistocalyx operculatus flower buds have been widely used in traditional medicine because of their rich content of bioactive constituents. In this study, we obtained seven solvent extracts from the flower buds and evaluated their total phenolic (TPC), flavonoid (TFC), tannin (TTC), triterpenoid saponin (TSC), and alkaloid (TAC) contents. We assessed antioxidant activities using the DPPH assay and also looked at antimicrobial and enzyme inhibitory effects. The water extract possessed the highest TPC (328.9 mg GAE/g extract), followed by ethanol, methanol, and hexane extracts (85.4-101.5 mg GAE/g extract). Chloroform, butanol, ethyl acetate, and ethanol extracts had high TSCs (245.4-287.2 mg OAE/g extract). The hexane extract was richest in TTC and TFC (32.7 mg CE/g extract and 81.1 mg QE/g extract, respectively). Ethanol and methanol extracts exhibited the strongest antioxidant activities (IC50 values of 25.2 and 30.3 μg/ml, respectively), followed by the water extract (IC50 of 40.2 μg/ml). The hexane extract displayed the most growth-inhibitory activity against Helicobacter pylori ATCC51932 and ATCC43504 strains and Salmonella enterica serovar Typhimurium ATCC13311 (MIC values of 0.06, 0.13, and 0.4 mg/ml, respectively). Moreover, the hexane extract revealed the strongest inhibition of H. pylori urease activity (IC50 of 4.51 μg/ml), whereas the water and methanol extracts had potent inhibitory effects on α-glucosidase activity (IC50 values of 9.9 and 15.1 μg/ml, respectively). These flower bud extracts could be used for health protection, especially in preventing bacterial infections and inhibiting enzymes associated with various human diseases. Further investigation into the application of C. operculatus flower buds in the food and pharmaceutical industries is necessary.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 2","pages":"137-147"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11231999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0