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The International journal of developmental biology最新文献

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SV40T reprograms Schwann cells into stem-like cells that can re-differentiate into terminal nerve cells. SV40T将雪旺细胞重编程为干细胞样细胞，可以重新分化为末梢神经细胞。
The International journal of developmental biology Pub Date : 2020-02-26 DOI: 10.21203/rs.2.24550/v1
Rui-fang Li, Guo-xin Nan, Dan Wang, Chang Gao, Juan Yang, T. He, Zhong-lin Zhang
{"title":"SV40T reprograms Schwann cells into stem-like cells that can re-differentiate into terminal nerve cells.","authors":"Rui-fang Li, Guo-xin Nan, Dan Wang, Chang Gao, Juan Yang, T. He, Zhong-lin Zhang","doi":"10.21203/rs.2.24550/v1","DOIUrl":"https://doi.org/10.21203/rs.2.24550/v1","url":null,"abstract":"BACKGROUND\u0000The specific effect of SV40T on neurocytes has been rarely investigated by the researchers. We transfected Schwann cells (SCs) that did not have differentiation ability with MPH 86 plasmid containing SV40T in order to explore the effects of SV40T on Schwann cells.\u0000\u0000\u0000METHODS\u0000SCs were transfected with MPH 86 plasmid carrying the SV40T gene and cultured in different media, as well as co-cultured with neural stem cells (NSCs). In our study, SCs overexpressing SV40T were defined as SV40T-SCs. The proliferation of these cells was detected by WST-1, and the expression of different biomarkers was analyzed by qPCR and immunohistochemistry.\u0000\u0000\u0000RESULTS\u0000SV40T induced the characteristics of NSCs, such as the ability to grow in suspension, form spheroid colonies and proliferate rapidly, in the SCs, which were reversed by knocking out SV40T by the Flip-adenovirus. In addition, SV40T upregulated the expressions of neural crest-associated markers Nestin, Pax3 and Slug, and down-regulated S100b as well as the markers of mature SCs MBP, GFAP and Olig1/2. These cells also expressed NSC markers like Nestin, Sox2, CD133 and SSEA-1, as well as early development markers of embryonic stem cells (ESCs) like BMP4, c-Myc, OCT4 and Gbx2. Co-culturing with NSCs induced differentiation of the SV40T-SCs into neuronal and glial cells.\u0000\u0000\u0000CONCLUSIONS\u0000SV40T reprograms Schwann cells to stem-like cells at the stage of neural crest cells (NCCs) that can differentiate to neurocytes.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84277330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Memoriam - Antony Durston.
The International journal of developmental biology Pub Date : 2020-01-01 DOI: 10.1387/ijdb.200236vn
M. Cohen, V. Nanjundiah, C. Weijer, K. Zhu
{"title":"In Memoriam - Antony Durston.","authors":"M. Cohen, V. Nanjundiah, C. Weijer, K. Zhu","doi":"10.1387/ijdb.200236vn","DOIUrl":"https://doi.org/10.1387/ijdb.200236vn","url":null,"abstract":"Antony Durston, Tony to friends and colleagues, died on February 21, 2020 following sepsis caused by an underlying medical condition. He made important and highly original contributions to our understanding of the principles that underlie multicellular organisation and development (see Supplementary Material). The attitude which he brought to bear while doing science is as noteworthy as his research. What follows is a brief sketch of his career and persona. After obtaining a Bachelor of Science degree with Botany as his major from the University of Nottingham in 1965, Tony joined Neville Symonds to do a PhD in bacteriophage genetics at the University of Sussex, where he was influenced as well by Brian Goodwin and John Maynard Smith. It was Symonds who inspired him to develop his natural tendency to think outside the box.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"62 1","pages":"393-395"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72646487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chick midgut morphogenesis. 鸡中肠形态发生。
IF 0.7
The International journal of developmental biology Pub Date : 2018-01-01 DOI: 10.1387/ijdb.170325ct
Tyler R Huycke, Clifford J Tabin
{"title":"Chick midgut morphogenesis.","authors":"Tyler R Huycke,&nbsp;Clifford J Tabin","doi":"10.1387/ijdb.170325ct","DOIUrl":"https://doi.org/10.1387/ijdb.170325ct","url":null,"abstract":"<p><p>The gastrointestinal tract is an essential system of organs required for nutrient absorption. As a simple tube early in development, the primitive gut is patterned along its anterior-posterior axis into discrete compartments with unique morphologies relevant to their functions in the digestive process. These morphologies are acquired gradually through development as the gut is patterned by tissue interactions, both molecular and mechanical in nature, involving all three germ layers. With a focus on midgut morphogenesis, we review work in the chick embryo demonstrating how these molecular signals and mechanical forces sculpt the developing gut tube into its mature form. In particular, we highlight two mechanisms by which the midgut increases its absorptive surface area: looping and villification. Additionally, we review the differentiation and patterning of the intestinal mesoderm into the layers of smooth muscle that mechanically drive peristalsis and the villification process itself. Where relevant, we discuss the mechanisms of chick midgut morphogenesis in the context of experimental data from other model systems.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"109-119"},"PeriodicalIF":0.7,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1387/ijdb.170325ct","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35975418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
A snail tale and the chicken embryo. 蜗牛故事和鸡胚。
IF 0.7
The International journal of developmental biology Pub Date : 2018-01-01 DOI: 10.1387/ijdb.170301mn
M Angela Nieto
{"title":"A snail tale and the chicken embryo.","authors":"M Angela Nieto","doi":"10.1387/ijdb.170301mn","DOIUrl":"https://doi.org/10.1387/ijdb.170301mn","url":null,"abstract":"<p><p>Some 25 years ago, a clone was identified that contained the chicken Slug sequences (now called Snail2 ). How could we anticipate at that time how much the chick embryo would help us to understand the ins and outs of cell migration during development and in disease? Indeed, the chick embryo helped us identify Snail2 as the first transcription factor that could induce the epithelial-mesenchymal transition (EMT), key for the migration of embryonic and cancer cells.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"121-126"},"PeriodicalIF":0.7,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1387/ijdb.170301mn","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35975419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
From soil mechanics to chick development. 从土壤力学到小鸡发育。
IF 0.7
The International journal of developmental biology Pub Date : 2018-01-01 DOI: 10.1387/ijdb.180030LW
Lewis Wolpert
{"title":"From soil mechanics to chick development.","authors":"Lewis Wolpert","doi":"10.1387/ijdb.180030LW","DOIUrl":"https://doi.org/10.1387/ijdb.180030LW","url":null,"abstract":"<p><p>Here, I provide some recollections of my life, starting as a civil engineer in South Africa and how I gradually became interested in biology, particularly pattern formation. In retrospect, I think that my decision to work on chick embryos to study limb development back in 1966 turned out to be the right one. The principles discovered in these 50 years, both by my collaborators and by other colleagues, have established the principles of how the limb develops in higher vertebrates, including humans.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"35-41"},"PeriodicalIF":0.7,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1387/ijdb.180030LW","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35975760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Insights into neural crest development from studies of avian embryos. 从鸟类胚胎研究中了解神经嵴发育。
IF 0.7
The International journal of developmental biology Pub Date : 2018-01-01 DOI: 10.1387/ijdb.180038sg
Shashank Gandhi, Marianne E Bronner
{"title":"Insights into neural crest development from studies of avian embryos.","authors":"Shashank Gandhi,&nbsp;Marianne E Bronner","doi":"10.1387/ijdb.180038sg","DOIUrl":"https://doi.org/10.1387/ijdb.180038sg","url":null,"abstract":"<p><p>The neural crest is a multipotent and highly migratory cell type that contributes to many of the defining features of vertebrates, including the skeleton of the head and most of the peripheral nervous system. 150 years after the discovery of the neural crest, avian embryos remain one of the most important model organisms for studying neural crest development. In this review, we describe aspects of neural crest induction, migration and axial level differences, highlighting what is known about the underlying gene regulatory mechanisms. Past and emerging technologies continue to improve the resolution with which we can examine important questions of neural crest development, with modern avian molecular embryology continuing to make important contributions.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"183-194"},"PeriodicalIF":0.7,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1387/ijdb.180038sg","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35975868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Specification of sensory placode progenitors: signals and transcription factor networks. 感觉基板祖细胞的规范:信号和转录因子网络。
IF 0.7
The International journal of developmental biology Pub Date : 2018-01-01 DOI: 10.1387/ijdb.170298as
Andrea Streit
{"title":"Specification of sensory placode progenitors: signals and transcription factor networks.","authors":"Andrea Streit","doi":"10.1387/ijdb.170298as","DOIUrl":"https://doi.org/10.1387/ijdb.170298as","url":null,"abstract":"<p><p>Sensory placodes contribute to much of the sensory nervous system in the vertebrate head. They give rise to parts of the eye, ear and nose, as well as to the sensory ganglia that innervate the face, tongue, oesophagus and visceral tissues. Despite their diversity, during development placodes arise from a population of common progenitor cells, which are first specified at the border of the neural plate. The chick has been particularly instrumental in dissecting the timing of these events, and recent evidence has highlighted the close relationship of placode progenitors and precursors for neural crest cells and the central nervous system. This review focuses on the induction of placode progenitors by localised signalling events, and the transcriptional networks that lead to their specification.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"195-205"},"PeriodicalIF":0.7,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1387/ijdb.170298as","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35975870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
General principles of spinal motor circuit development: early contributions from research on avian embryos. 脊柱运动回路发育的一般原理:来自禽类胚胎研究的早期贡献。
IF 0.7
The International journal of developmental biology Pub Date : 2018-01-01 DOI: 10.1387/ijdb.170305LL
Lynn T Landmesser
{"title":"General principles of spinal motor circuit development: early contributions from research on avian embryos.","authors":"Lynn T Landmesser","doi":"10.1387/ijdb.170305LL","DOIUrl":"https://doi.org/10.1387/ijdb.170305LL","url":null,"abstract":"<p><p>Birds and mammals, both being amniotes, share many common aspects of development. Thus our understanding of how limb-innervating mammalian spinal motor circuits develop was greatly influenced by the use of the avian embryo (chick/quail) to bring about experimental perturbations to identify basic underlying mechanisms. These included embryonic surgery, the application of drugs to influence activity or molecular interactions, and the ability to observe motor behavior and make physiological recordings in intact developing embryos. This article will review some of these contributions, highlighting several areas including the acquisition of motoneuron subtype identity and target selection, as well as the role of spontaneous rhythmic activity in circuit development.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"235-243"},"PeriodicalIF":0.7,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1387/ijdb.170305LL","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35975873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Sex determination and gonadal sex differentiation in the chicken model. 鸡模型的性别决定和性腺性别分化。
IF 0.7
The International journal of developmental biology Pub Date : 2018-01-01 DOI: 10.1387/ijdb.170319cs
Claire E Hirst, Andrew T Major, Craig A Smith
{"title":"Sex determination and gonadal sex differentiation in the chicken model.","authors":"Claire E Hirst,&nbsp;Andrew T Major,&nbsp;Craig A Smith","doi":"10.1387/ijdb.170319cs","DOIUrl":"https://doi.org/10.1387/ijdb.170319cs","url":null,"abstract":"<p><p>Our understanding of avian sex determination and gonadal development is derived primarily from the studies in the chicken. Analysis of gynandromorphic chickens and experimental chimeras indicate that sexual phenotype is at least partly cell autonomous in the chicken, with sexually dimorphic gene expression occurring in different tissue and different stages. Gonadal sex differentiation is just one of the many manifestations of sexual phenotype. As in other birds, the chicken has a ZZ male: ZW female sex chromosome system, in which the male is the homogametic sex. Most evidence favours a Z chromosome dosage mechanism underling chicken sex determination, with little evidence of a role for the W chromosome. Indeed, the W appears to harbour a small number of genes that are un-related to sexual development, but have been retained because they are dosage sensitive factors. As global Z dosage compensation is absent in birds, Z-linked genes may direct sexual development in different tissues (males having on average 1.5 to 2 times the expression level of females). In the embryonic gonads, the Z-linked DMRT1 gene plays a key role in testis development. Beyond the gonads, other combinations of Z-linked genes may govern sexual development, together with a role for sex steroid hormones. Gonadal DMRT1 is thought to activate other players in testis development, namely SOX9 and AMH, and the recently identified HEMGN gene. DMRT1 also represses ovarian pathway genes, such as FOXL2 and CYP19A1. A lower level of DMRT1 expression in the female gonads is compatible with activation of the ovarian pathway. Some outstanding questions include how the key testis and ovary genes, DMRT1 and FOXL2, are regulated. In addition, confirmation of the central role of these genes awaits genome editing approaches.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"153-166"},"PeriodicalIF":0.7,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1387/ijdb.170319cs","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35975424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 51
Chicken genomics. 鸡基因组学。
IF 0.7
The International journal of developmental biology Pub Date : 2018-01-01 DOI: 10.1387/ijdb.170276yc
Yuanyuan Cheng, David W Burt
{"title":"Chicken genomics.","authors":"Yuanyuan Cheng,&nbsp;David W Burt","doi":"10.1387/ijdb.170276yc","DOIUrl":"https://doi.org/10.1387/ijdb.170276yc","url":null,"abstract":"<p><p>As one of the most economically important species and a unique model organism for biological and medical research, the chicken represents the first non-mammalian amniotic species to have its genome sequenced; and so far, the chicken reference genome represents the best assembled and annotated avian genome. Since the release of the first draft genome sequence, the chicken genome assembly has improved greatly in coverage, contiguity and accuracy owing to the continuous efforts made by the chicken genomics community to generate extensive new data using novel sequencing technologies. Transcriptome sequencing, especially the recent effort to characterise full-length transcripts in chicken tissues, has provided key insights into the complexity of structure and function of the chicken genome. In this article, we review the progress in chicken genome assembly and annotation, and recent advances in comparative genomics in birds. Limitations of current data and plans of research are also discussed.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"265-271"},"PeriodicalIF":0.7,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1387/ijdb.170276yc","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35975759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
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