{"title":"小鼠卵母细胞成熟过程中CDK1蛋白与CDK1 mRNA的相关性研究。","authors":"Ya-Ting Sun, Ai-Zhen Zhu","doi":"10.1387/ijdb.220002za","DOIUrl":null,"url":null,"abstract":"<p><p>The aim of this study was to investigate the correlation between CDK1 protein and CDK1 mRNA during oocyte maturation <i>in vivo</i> in mouse. GV, GVBD, MI and MII oocytes were obtained from mice, respectively. Western blot validated that the CDK1 protein expression increased continuously and significantly with oocyte maturation <i>in vivo</i> (P<0.05). Real-time qRT-PCR showed that CDK1 mRNA expression was down-regulated significantly during transformation from GV to MI stages (P<0.05), and up-regulated significantly during transformation from MI to MII stages (P<0.05). The level of CDK1 mRNA peaked at MII stages. Spearman correlation analysis indicated that CDK1 protein expression was poor correlation with CDK1 mRNA expression during oocyte maturation <i>in vivo</i> (R=0.200). This finding suggested that the increase of CDK1 protein during oocyte maturation <i>in vivo</i> was not entirely caused by the change of transcription level. The results provide new food for thought for further research on the molecular mechanism of oocyte maturation <i>in vivo</i>.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"305-309"},"PeriodicalIF":0.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Correlation between CDK1 protein and CDK1 mRNA during oocyte maturation in mouse.\",\"authors\":\"Ya-Ting Sun, Ai-Zhen Zhu\",\"doi\":\"10.1387/ijdb.220002za\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The aim of this study was to investigate the correlation between CDK1 protein and CDK1 mRNA during oocyte maturation <i>in vivo</i> in mouse. GV, GVBD, MI and MII oocytes were obtained from mice, respectively. Western blot validated that the CDK1 protein expression increased continuously and significantly with oocyte maturation <i>in vivo</i> (P<0.05). Real-time qRT-PCR showed that CDK1 mRNA expression was down-regulated significantly during transformation from GV to MI stages (P<0.05), and up-regulated significantly during transformation from MI to MII stages (P<0.05). The level of CDK1 mRNA peaked at MII stages. Spearman correlation analysis indicated that CDK1 protein expression was poor correlation with CDK1 mRNA expression during oocyte maturation <i>in vivo</i> (R=0.200). This finding suggested that the increase of CDK1 protein during oocyte maturation <i>in vivo</i> was not entirely caused by the change of transcription level. The results provide new food for thought for further research on the molecular mechanism of oocyte maturation <i>in vivo</i>.</p>\",\"PeriodicalId\":94228,\"journal\":{\"name\":\"The International journal of developmental biology\",\"volume\":\" \",\"pages\":\"305-309\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The International journal of developmental biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1387/ijdb.220002za\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The International journal of developmental biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1387/ijdb.220002za","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
本研究旨在探讨小鼠体内卵母细胞成熟过程中CDK1蛋白与CDK1 mRNA的相关性。分别从小鼠获得GV、GVBD、MI和MII卵母细胞。Western blot验证了CDK1蛋白在体内随着卵母细胞成熟持续显著升高(Pin - vivo (R=0.200))。这一发现提示，体内卵母细胞成熟过程中CDK1蛋白的增加并不完全是由转录水平的变化引起的。这些结果为进一步研究卵母细胞在体内成熟的分子机制提供了新的思路。
Correlation between CDK1 protein and CDK1 mRNA during oocyte maturation in mouse.
The aim of this study was to investigate the correlation between CDK1 protein and CDK1 mRNA during oocyte maturation in vivo in mouse. GV, GVBD, MI and MII oocytes were obtained from mice, respectively. Western blot validated that the CDK1 protein expression increased continuously and significantly with oocyte maturation in vivo (P<0.05). Real-time qRT-PCR showed that CDK1 mRNA expression was down-regulated significantly during transformation from GV to MI stages (P<0.05), and up-regulated significantly during transformation from MI to MII stages (P<0.05). The level of CDK1 mRNA peaked at MII stages. Spearman correlation analysis indicated that CDK1 protein expression was poor correlation with CDK1 mRNA expression during oocyte maturation in vivo (R=0.200). This finding suggested that the increase of CDK1 protein during oocyte maturation in vivo was not entirely caused by the change of transcription level. The results provide new food for thought for further research on the molecular mechanism of oocyte maturation in vivo.
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