斑马鱼胚胎发生过程中前列腺素E受体2a和4b亚型的分子特征及其表达模式

Yongjun Han, Hongbo Chang, Hong Wu
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摘要

迄今为止,斑马鱼ep2a和ep4b的分子表达谱尚未确定。斑马鱼和其他物种EP2a和EP4b的系统发育树显示,人类EP4和斑马鱼EP4b的亲缘关系比EP2a更近。斑马鱼EP2a是一种281个氨基酸的蛋白,与人类(43%)、小鼠(44%)、大鼠(43%)、狗(44%)、牛(41%)、鸡(41%)具有较高的同源性。斑马鱼EP4b编码497个氨基酸前体,与哺乳动物(人类57%)、小鼠(54%)、大鼠(55%)、狗(55%)、牛(56%)和鸡(54%)具有较高的氨基酸同源性。全安装原位杂交显示,ep2a在10个体体阶段的前4个体体中强烈表达,但在19 hpf时的体体中不存在。在24 hpf时肾原管、位于干部的中间细胞团和30 hpf时吻侧血岛均可见。在48 hpf时,脊索中也有Ep2a的表达。在体细胞发生过程中,ep4b在眼睛、体细胞和主干神经嵴高度表达。在30 ~ 48 hpf范围内,ep4b可在后主静脉及邻近的ICM中检测到。根据这些数据,我们得出结论,ep2a和ep4b在脊椎动物中是保守的,ep2a和ep4b转录本在发育阶段的存在推断了它们在早期斑马鱼幼虫发育中的作用。此外,两种受体异构体的可变表达强烈暗示了分子调控的不同作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular characterization of the prostaglandin E receptor subtypes 2a and 4b and their expression patterns during embryogenesis in zebrafish.
The molecular expression profiles of zebrafish ep2a and ep4b have not been defined to-date. Phylogenetic trees of EP2a and EP4b in zebrafish and other species revealed that human EP4 and zebrafish EP4b were more closely related than EP2a. Zebrafish EP2a is a 281 amino acid protein with high identity to that of human (43%), mouse (44%), rat (43%), dog (44%), cattle (41%), and chicken (41%). Zebrafish EP4b encoded a precursor of 497 amino acids with high amino acid identity to that of mammals, including human (57%), mouse (54%), rat (55%), dog (55%), cattle (56%), and chicken (54%). Whole-mount in situ hybridization revealed that ep2a was robustly expressed in the anterior four somites at the 10-somites stages, but was absent in the somites at 19 hpf. It was observed again in the pronephric duct at 24 hpf, in the intermediate cell mass located in the trunk, and in the rostral blood island at 30 hpf. Ep2a was also expressed in the notochord at 48 hpf. During somitogenesis, ep4b was highly expressed in the eyes, somites, and the trunk neural crest. From 30 to 48 hpf, ep4b could be detected in the posterior cardinal vein and the neighboring ICM. From these data, we conclude that ep2a and ep4b are conserved in vertebrates and that the presence of ep2a and ep4b transcripts during developmental stages infers their role during early zebrafish larval development. In addition, the variable expression of the two receptor isoforms was strongly suggestive of divergent roles of molecular regulation.
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