The International journal of developmental biology最新文献

{"title":"Polarized contact behavior in directionally migrating <i>Xenopus</i> gastrula mesendoderm.","authors":"Martina Nagel, Rudolf Winklbauer","doi":"10.1387/ijdb.230123rw","DOIUrl":"10.1387/ijdb.230123rw","url":null,"abstract":"<p><p>The control of cell-cell adhesion and detachment is essential for collective migration and cell rearrangement. Here, we have used the contact behavior of <i>Xenopus</i> gastrula mesoderm explants migrating directionally on ectoderm conditioned substratum to study the regulation of active cell-cell detachment. When colliding laterally, explants repelled each other, whereas they fused front-to-back when aligned in the direction of migration. For this mesoderm polarization by the substratum, we identified three control modules. First, PDGF-A signaling normally suppresses contact-induced collapse of lamellipodia in a polarized manner. Disruption of PDGF-A function, or of Xwnt6, decreased the polarization of explant contact behavior. Second, the Wnt receptor Xfz7 acted upstream of the kinase Pak1 to control explant fusion independently of PDGF-A-promoted lamellipodia stability. Third, ephrinB1 acted with Dishevelled (Dvl) in front-to-back explant fusion. The second and third modules have been identified previously as regulators of tissue separation at the ectoderm-mesoderm boundary. On non-polarizing, fibronectin-coated substratum, they controlled repulsion between explants in the same way as between tissues during boundary formation. However, explant repulsion/fusion responses were reversed on conditioned substratum by the endogenous guidance cues that also control oriented contact inhibition of lamellipodia. Together, control modules and substratum-bound guidance cues combine preferential front-back adhesion and diminished lateral adhesion of cells to promote collective directional mesoderm migration.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"79-90"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41171630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the role of the Wnt signaling pathway in the regeneration of animal model systems.","authors":"Katarzyna S Walczyńska, Ling Zhu, Yujun Liang","doi":"10.1387/ijdb.220144yl","DOIUrl":"10.1387/ijdb.220144yl","url":null,"abstract":"<p><p>Regeneration enables the regrowth and restoration of missing body parts. It is a common phenomenon among animals. However, only some species exhibit remarkable regeneration capabilities and can regenerate organs such as limbs, lenses or hearts. Regeneration has been widely studied, thereby giving rise to new fields, such as regenerative medicine. Furthermore, regeneration has the potential to be applied to the human body. However, the molecular mechanisms governing this process should be elucidated first. Recent advancements in research methods have led to the identification of numerous signaling pathways involved in regeneration. One of them, the Wnt transduction pathway, is an ancient and evolutionarily conserved pathway that plays an important role in both embryonic development and regeneration. The Wnt pathway plays an important role during the regeneration process, as it is implicated in cell fate determination, cell migration, cell polarity and adult cell homeostasis. To date, two major Wnt pathways have been identified: the canonical (β-catenin dependent) pathway and the non-canonical pathway. The latter pathway can be further divided into planar cell polarity, the Wnt/Ca2+ pathway and the JNK pathway. In this review, we summarize the current state of knowledge regarding the Wnt signaling pathway and its role in regeneration, with a particular emphasis on key model species.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"67 3","pages":"65-78"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71490792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental Biology: from genes to functional organism - news from the 3^rd Meeting of the Visegrád Group Society for Developmental Biology.","authors":"Ewelina Trela, Agnieszka Walewska","doi":"10.1387/ijdb.230228et","DOIUrl":"10.1387/ijdb.230228et","url":null,"abstract":"<p><p>The third meeting of the Visegrád Group Society for Developmental Biology (V4SDB) was held on September 8^th-10^th, 2023 in Warsaw, Poland. It was a continuation of previous meetings, the first organized in the Czech Republic in 2018 and the second in Hungary in 2021. Similarly to the previous meetings, the organizers created a friendly platform for networking and science sharing. The conference gathered an excellent group of 160 researchers working on various animal models, who during lecture and poster sessions discussed a broad range of subjects, including early embryonic development, organogenesis, genetic and epigenetic control of developmental processes, stem cells and regeneration, cellular dynamics and migration in developmental biology, and <i>in vitro</i> models in development and disease. Additionally, two satellite events were organized: the Young Developmental Biologists' Forum, which gave young researchers an opportunity to share and promote their work and to participate in hands-on courses, and an outreach initiative \"Developmental Biology for Everyone\", which presented different aspects of developmental biology to a broad audience.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"109-114"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139089805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strontium-doped hydroxyapatite and its role in osteogenesis and angiogenesis","authors":"Ling Ran, Lishuang Liu, Jingjing Gao, Yang Pan, Murugan Ramalingam, Xiaoyu Du, Ying Liu, Lijia Cheng, Zheng Shi","doi":"10.1387/ijdb.230091lc","DOIUrl":"https://doi.org/10.1387/ijdb.230091lc","url":null,"abstract":"For the past 50 years, hydroxyapatite (HA) has been widely used in bone defect repair because it is the main inorganic component of the mineral phase of a human bone. Extensive preclinical and clinical studies have shown that strontium (Sr) can safely and effectively help prevent and treat bone diseases, including osteoporosis. These findings have resulted in the concept of integrating Sr and HA for bone disease management. The doped Sr can improve the physicochemical properties of HA and enhance its angiogenic and bone regeneration ability. Nevertheless, no study has reviewed the design strategy of Sr-doped HA (Sr-HA) to understand its biological roles. Therefore, in this article, we review recent developments in Sr-HA preparation and its effect on osteogenesis and angiogenesis in vitro and in vivo along with key suggestions for future research and development.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135705501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterogeneity of quiescent and active neural stem cells in the postnatal brain.","authors":"Dimitrios Dimitrakopoulos, D. Kakogiannis, I. Kazanis","doi":"10.1387/ijdb.220010ik","DOIUrl":"https://doi.org/10.1387/ijdb.220010ik","url":null,"abstract":"In the postnatal mammalian brain, neurogenic activity is retained in anatomically restricted areas, driven by pools of Neural Stem Cells (NSCs). These cells and their progeny have been studied intensively as potential targets for regenerative treatments, aiming either to their in situmanipulation, or to their use as sources of cells for transplantation-based strategies. Although their full identity, heterogeneity and differentiation potential remain elusive, due to the absence of specific cell-type markers, our knowledge on their properties is constantly expanding. Here, we focus on the NSC niche that is located at the Subependymal Zone (SEZ/ also known as Subventricular Zone) of the lateral ventricles of the brain. We review, summarize and explain the different faces of the NSC, as they have been described using a wide range of experimental approaches in a time-frame of three decades: the primitive, definitive, quiescent or activated NSC. We also review the accumulating evidence on the existence of latent NSCs outside of niches, in the brain parenchyma, that constitute new promising therapeutic targets, complemented by the novel technologies of in vivocell reprogramming.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88124798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epithelial endothelial transition and endothelial mesenchymal transition.","authors":"D. Ribatti","doi":"10.1387/ijdb.210234dr","DOIUrl":"https://doi.org/10.1387/ijdb.210234dr","url":null,"abstract":"The movement of continuous sheets of epithelial cells occurs during embryonic development, tissue repair, and cancer. Common to cellular and molecular principles of collective cell migration, invading cancers seem to reactivate embryonic pathways and patterns of cell movement. Epithelial cells possess the capability to become mesenchymal cells in a process called epithelial mesenchymal transition (EMT), which has been extensively studied and described. The aim of this article is to summarizes the most recent literature data concerning less known epithelial-endothelial transition and endothelial-mesenchymal transition.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76497210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronan receptor CD44: developmentally regulated expression and role in the early chick embryo.","authors":"K. Konstantopoulos, Alexandros Dimiropoulos, N. Zagris","doi":"10.1387/ijdb.220008nz","DOIUrl":"https://doi.org/10.1387/ijdb.220008nz","url":null,"abstract":"CD44 is a membrane glycoprotein and is the main receptor for hyaluronan. We studied the CD44 expression and spatio-temporal distribution by RT-PCR and immunofluorescence, and used an anti-CD44 blocking antibody to perturb CD44-depended signalling programs in the early chick embryo. The intense CD44 levels we detected in the morula embryo (XI) were of novel interest suggestive of a maternally stored transcript. Intriguingly, the CD44 early presence seemed to be essential for the rapid synthesis of hyaluronan. At stage XIII (blastula), CD44 expression was intense in the epiblast and hypoblast. During gastrulation (HH3-4), the cells ingressing into the primitive groove and migrating and the blood islands expressed CD44 intensely. At HH8, the folding neural plate showed polarity regulation of CD44 expression, and expression was also intense in neural crest, notochord, and blood islands. During early organogenesis, CD44 was expressed intensely in the developing cranial and caudal neural tube which showed polarity regulation, in optic stalks, otic vesicles, pre-and migratory neural crest cells, ganglia, notochord, pharynx, gut, liver, aortae, heart, somites, vascular area, amnion, chorion and was distinct in extracellular matrix of cranial neural tube and otic vesicle lumens. Antibody-mediated perturbation of CD44 function resulted in unorganized extracellular matrix, loss of tissue spaces, grossly abnormal notochord, intermingling of clumped neuroectoderm and mesenchyme, absence of somites and blood vessels, inhibition of neural crest cell emigration. CD44 has various pivotal roles in matrix integrity and tissue patterning consistent with its known biochemical features and interactions with hyaluronan, growth factors, receptors and other signaling molecules.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78362851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pleiotrophin, nitric oxide and glutamate AMPA receptors in chick cerebellum morphogenesis.","authors":"Vasiliki Kommata, Evaggelia Alexopoulou, Elentina K. Argyrousi, C. Dermon","doi":"10.1387/ijdb.210213cd","DOIUrl":"https://doi.org/10.1387/ijdb.210213cd","url":null,"abstract":"Avian cerebellum, a highly conserved, laminated and foliated structure, provides an excellent model for developmental studies. During the intermediate embryonic stages, granule cell progenitor proliferation and the inwards migration of post-mitotic granule cells have been implicated in the morphogenesis of cerebellar cortex cytoarchitecture and foliation. The present study questioned the spatio-temporal expression pattern of pleiotrophin, an extracellular matrix growth factor, during the morphogenesis of embryonic cerebellum and the roles of ionotropic AMPA glutamate receptors and the diffusible neuromodulator nitric oxide (NO) in the proliferation pattern of EGL granule cell progenitors. For this, the density of proliferating cells in the developing embryonic external granule layer (EGL) was determined following acute treatment with AMPA receptor antagonist CNQX or NO synthase inhibitor L-NAME, at embryonic stages HH38-41 (E12-E15 days), by means of BrdU immunohistochemistry and double immunofluorescence. Importantly, at earlier stages pleiotrophin-like immunoreactivity showed high expression levels in the EGL that gradually decreased, persisting within the growing folia apices, later in development. Interestingly, blockage of AMPA receptors had no effect; while NOS inhibition resulted in transient age- and region-specific increases of EGL granule progenitor cell proliferation at earlier stages, but decreased the post-mitotic granule cells at folia apices, at a later stage HH41 (E15 day). Taken all together, NO had a transient anti-proliferative effect in EGL similar to mammalian cerebellum, acting as a modulator of the EGL function at different stages, suggesting its possible implication in complex processes guiding cerebellar cytoarchitecture and folia formation.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87368888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the sister cells of embryo sac: developmental and functional attributes.","authors":"Inderdeep Kaur,&nbsp;Monika Koul","doi":"10.1387/ijdb.220025mk","DOIUrl":"https://doi.org/10.1387/ijdb.220025mk","url":null,"abstract":"<p><p>Synergids are metabolically dynamic cells of the egg apparatus and represent an important component of the female gametophyte. Besides directing the growth of the pollen tube towards the micropylar end of the embryo sac, these ephemeral structures make room for the pollen tube cytoplasm. The nature of chemotrophic substances that direct the growth of the pollen tube, the mechanism of degeneration of one of the synergids before fertilization and the molecular aspects of synergid morphogenesis have been studied in detail. Research carried out on model systems such as <i>Arabidopsis, Brassica, Capsella, Triticum</i> and <i>Torenia</i> has expanded our understanding of the molecular regulation of the pollen tube journey, its guidance and navigation in the pistil. Recently, the critical role of the central cell in fertilization and prevention of polytubey has also been thoroughly investigated. Interesting aspects that lead to degeneration of synergids, and the factors governing degeneration, including molecular aspects, have produced a paradigm shift in the understanding of these intriguing units. Sophisticated confocal microscopy, live cell imaging, and molecular tools have helped in furthering our knowledge of the functioning of synergids. Recent research using high throughput techniques has deciphered the role of various genes that regulate and govern the release of chemotropic substances, cell-to-cell interaction and synergid cell degeneration. Moreover, with the diversity displayed in form and function of organs in the angiosperms, and the switching of roles of the cells of egg apparatus, new insights have been provided into the involvement of synergids both pre- and post-fertilization. The present review provides a comprehensive account of synergids, their role in fertilization and the post fertilization events that have emerged using interdisciplinary approaches in recent years. We also discuss the variations observed in degeneration of synergids and the mechanisms that have been unraveled recently. Study of the dynamism exhibited by synergids reveals newer roles of these in fertilization. How synergids in angiosperm taxa where genetic transformation/alteration is carried out will respond to pollen stimuli is still unknown. Since environmental factors such as light and temperature have a significant impact on synergids and fertilization, it would be rewarding to study the role of chemo-attractants and other factors in elucidating the functional roles of synergids. Further research into developing adequate protocols for manipulating synergid functions is certainly required. This research has enormous potential in the advancement of basic science and has potential applications in agriculture, horticulture, and bioprospecting.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"349-358"},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40720125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between CDK1 protein and CDK1 mRNA during oocyte maturation in mouse.","authors":"Ya-Ting Sun,&nbsp;Ai-Zhen Zhu","doi":"10.1387/ijdb.220002za","DOIUrl":"https://doi.org/10.1387/ijdb.220002za","url":null,"abstract":"<p><p>The aim of this study was to investigate the correlation between CDK1 protein and CDK1 mRNA during oocyte maturation <i>in vivo</i> in mouse. GV, GVBD, MI and MII oocytes were obtained from mice, respectively. Western blot validated that the CDK1 protein expression increased continuously and significantly with oocyte maturation <i>in vivo</i> (P<0.05). Real-time qRT-PCR showed that CDK1 mRNA expression was down-regulated significantly during transformation from GV to MI stages (P<0.05), and up-regulated significantly during transformation from MI to MII stages (P<0.05). The level of CDK1 mRNA peaked at MII stages. Spearman correlation analysis indicated that CDK1 protein expression was poor correlation with CDK1 mRNA expression during oocyte maturation <i>in vivo</i> (R=0.200). This finding suggested that the increase of CDK1 protein during oocyte maturation <i>in vivo</i> was not entirely caused by the change of transcription level. The results provide new food for thought for further research on the molecular mechanism of oocyte maturation <i>in vivo</i>.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"305-309"},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40407582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}