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The K129 capsular polysaccharide produced by Acinetobacter baumannii MAR 15–4076 has the same composition as K84 but differs in the linkage between units altering the overall branching topology 鲍曼不动杆菌 MAR 15-4076 产生的 K129 胶囊多糖与 K84 的成分相同,但在改变整体分支拓扑结构的单位之间的连接上存在差异
IF 2.4 3区 化学
Carbohydrate Research Pub Date : 2024-09-17 DOI: 10.1016/j.carres.2024.109273
Nikolay P. Arbatsky , Alexander S. Shashkov , Mikhail M. Shneider , Yulia V. Mikhailova , Andrey A. Shelenkov , Eugene A. Sheck , Anastasia A. Kasimova , Nadezhda A. Kalinchuk , Johanna J. Kenyon , Yuriy A. Knirel
{"title":"The K129 capsular polysaccharide produced by Acinetobacter baumannii MAR 15–4076 has the same composition as K84 but differs in the linkage between units altering the overall branching topology","authors":"Nikolay P. Arbatsky ,&nbsp;Alexander S. Shashkov ,&nbsp;Mikhail M. Shneider ,&nbsp;Yulia V. Mikhailova ,&nbsp;Andrey A. Shelenkov ,&nbsp;Eugene A. Sheck ,&nbsp;Anastasia A. Kasimova ,&nbsp;Nadezhda A. Kalinchuk ,&nbsp;Johanna J. Kenyon ,&nbsp;Yuriy A. Knirel","doi":"10.1016/j.carres.2024.109273","DOIUrl":"10.1016/j.carres.2024.109273","url":null,"abstract":"<div><div>Capsular polysaccharide (CPS) is a heteroglycan that coats the cell surface of most isolates of the important Gram-negative bacterial pathogen, <em>Acinetobacter baumannii.</em> Strain MAR 15–4076, a clinical isolate recovered in Russia in 2015, was found to carry the KL129 sequence at the CPS biosynthesis K locus. The CPS was isolated from the strain and studied by sugar analysis, Smith degradation, one- and two-dimensional <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy. It was composed of branched pentasaccharide units that include a →3)-α-<span>l</span>-Rha<em>p</em>-(1 → 3)-α-<span>l</span>-Rha<em>p</em>-(1 → 3)-β-<span>d</span>-Glc<em>p</em>NAc-(1→ mainchain and α-<span>d</span>-Man<em>p</em>NAc-(1 → 3)-<span>l</span>-Rha<em>p</em> side branch. Though the pentasaccharide units are identical to those that make up the K84 CPS produced by <em>A. baumannii</em> LUH5540, the units are linked differently via the substitution of an alternate <span>l</span>-Rha<em>p</em> residue, resulting in a difference in the overall topology of the CPS. This was due to the replacement of the Wzy polymerase gene encoded at the K locus.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"545 ","pages":"Article 109273"},"PeriodicalIF":2.4,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interaction between reacetylated chitosan and albumin in alcalescent media 再乙酰化壳聚糖和白蛋白在炼金介质中的相互作用
IF 2.4 3区 化学
Carbohydrate Research Pub Date : 2024-09-14 DOI: 10.1016/j.carres.2024.109277
Inesa V. Blagodatskikh , Oxana V. Vyshivannaya , Nikita A. Tishchenko , Evgeniya A. Bezrodnykh , Vladimir E. Piskarev , Rinat R. Aysin , Yurij A. Antonov , Victor N. Orlov , Vladimir E. Tikhonov
{"title":"Interaction between reacetylated chitosan and albumin in alcalescent media","authors":"Inesa V. Blagodatskikh ,&nbsp;Oxana V. Vyshivannaya ,&nbsp;Nikita A. Tishchenko ,&nbsp;Evgeniya A. Bezrodnykh ,&nbsp;Vladimir E. Piskarev ,&nbsp;Rinat R. Aysin ,&nbsp;Yurij A. Antonov ,&nbsp;Victor N. Orlov ,&nbsp;Vladimir E. Tikhonov","doi":"10.1016/j.carres.2024.109277","DOIUrl":"10.1016/j.carres.2024.109277","url":null,"abstract":"<div><p>Interaction of chitosan and its derivatives with proteins of animal blood at blood pH relevant conditions is of a particular interest for construction of antimicrobial chitosan/protein-based drug delivery systems. In this work, the interaction of a series of <em>N</em>-reacetylated oligochitosans (RA-CHI) having <em>M</em><sub><em>w</em></sub> of 10–12 kDa and differing in the degree of acetylation (DA 19, 24, and 40 %) with bovine serum albumin (BSA) in alkalescent media is described in first. It is shown that RA-CHI forms soluble complexes with BSA in solutions with pH 7.4 and a low ionic strength. Light scattering study shows that soluble RA-CHI complexes have spherical form with the radius of about 100 nm. Circular dichroism, fluorescent spectroscopy, and micro-IR spectroscopy studies show that the secondary structure of BSA in soluble complexes remain intact. Isothermal titration calorimetry of RA-CHI with DA 24 % and BSA mixing in the buffers with different ionization heats reveals a significant contribution of electrostatic forces to the binding process and an additional ionization of chitosan due to the proton transfer from the buffer substance. An increase of ionic strength to the blood relevant value 0.15 M suppresses the binding. It is shown that application of RA-CHI with higher DA value leads to a decrease in the affinity of RA-CHI to BSA and an alteration of the interaction mechanism. The finding opens an opportunity to the application of N-reacetylated chitosan derivatives in the complex systems compatible with blood plasma proteins.</p></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"545 ","pages":"Article 109277"},"PeriodicalIF":2.4,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regioselective sulfation of alginate at 2-O-position of mannuronic acid unit with Py∙SO3 in DMSO 在二甲基亚砜中用 Py∙SO3 在甘露糖酸单元的 2-O 位对海藻酸进行区域选择性硫酸化反应
IF 2.4 3区 化学
Carbohydrate Research Pub Date : 2024-09-14 DOI: 10.1016/j.carres.2024.109276
Karl Martin Ingerma , Indrek Reile , Rando Tuvikene
{"title":"Regioselective sulfation of alginate at 2-O-position of mannuronic acid unit with Py∙SO3 in DMSO","authors":"Karl Martin Ingerma ,&nbsp;Indrek Reile ,&nbsp;Rando Tuvikene","doi":"10.1016/j.carres.2024.109276","DOIUrl":"10.1016/j.carres.2024.109276","url":null,"abstract":"<div><p>Alginates are brown algal polysaccharides consisting of β-D-mannuronic (M) and α-<span>l</span>-guluronic acid (G) residues linked with 1→4 glycosidic bonds. To functionalize these natural resources for biomedical use, alginates can be chemically modified, including by sulfation. Here regioselective sulfation of alginates at M-2 in DMSO with Py∙SO<sub>3</sub> is described, by either sulfating alginates directly or through using alginates with added protecting groups (PG-s), including TBDMS-ether, Piv-, Bz-esters and intramolecular 3,6-lactone. Highest regioselectivity was found by sulfating TBDMS- and Piv-protected alginates, with over 65 % of M-residues being 2-<em>O</em>-sulfated. However significant reduction in molecular weight was found when alginates were sulfated in DMSO. Results from this work will allow a degree of control over substitution patterns in sulfated alginates. This will allow to more accurately determine structure-property relationships in biomedical research.</p></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"545 ","pages":"Article 109276"},"PeriodicalIF":2.4,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Towards the synthesis of a 2-deoxy-2-fluoro-d-mannose building block and characterisation of an unusual 2-S-phenyl anomeric pyridinium triflate salt via 1 → 2 S-migration 通过 1 → 2 S 迁移合成 2-脱氧-2-氟-d-甘露糖结构单元并鉴定不常见的 2-S-苯基异构体吡啶三酸盐
IF 2.4 3区 化学
Carbohydrate Research Pub Date : 2024-09-13 DOI: 10.1016/j.carres.2024.109275
Sean T. Evans , Graham J. Tizzard , Robert A. Field , Gavin J. Miller
{"title":"Towards the synthesis of a 2-deoxy-2-fluoro-d-mannose building block and characterisation of an unusual 2-S-phenyl anomeric pyridinium triflate salt via 1 → 2 S-migration","authors":"Sean T. Evans ,&nbsp;Graham J. Tizzard ,&nbsp;Robert A. Field ,&nbsp;Gavin J. Miller","doi":"10.1016/j.carres.2024.109275","DOIUrl":"10.1016/j.carres.2024.109275","url":null,"abstract":"<div><div>Regio- and stereo-selective synthetic routes to 2-deoxy-2-fluoro-<span>d</span>-mannose building blocks are often experimentally challenging when using Selectfluor with the corresponding glycal. We targeted a late-stage method to introduce fluorine in a stereospecific manner using inversion <em>via</em> a triflate. Accordingly, synthesis of a conventionally protected 2-deoxy-2-fluoro-<span>d</span>-mannose β-thioglycoside donor, directly applicable to oligosaccharide synthesis, was attempted using C2-triflate inversion of the corresponding <span>d</span>-glucoside with TBAF. Unexpectedly, an anomeric pyridinium salt was isolated when attempting to form the C2-triflate using Tf<sub>2</sub>O in pyridine. Indicatively, this proceeds <em>via</em> a 1 → 2 <em>S</em>-migration delivering a 1,2-<em>trans</em> product with α-<span>d</span>-manno configuration and the anomeric pyridinium in a pseudo-equatorial position. The structure of this unexpected intermediate was confirmed in the solid-state using X-ray crystallography. Omission of the pyridine solvent led to dimer formation. Switching the aglycone to an <em>O</em>-<em>para</em>-methoxyphenyl enabled smooth C2 inversion to the desired 2-deoxy-2-fluoro <span>d</span>-mannose system, suitably equipped for further anomeric manipulation.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"545 ","pages":"Article 109275"},"PeriodicalIF":2.4,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing the antibacterial activity of ampicillin loaded into chitosan/starch nanocomposites against AMR Staphylococcus aureus 增强壳聚糖/淀粉纳米复合材料负载的氨苄西林对金黄色葡萄球菌AMR的抗菌活性
IF 2.4 3区 化学
Carbohydrate Research Pub Date : 2024-09-13 DOI: 10.1016/j.carres.2024.109274
Vinh Nghi Nguyen , Van Bon Nguyen , Minh Dinh Tran , Manh Dung Doan , Dinh Sy Nguyen , Thi Huyen Nguyen , Chien Thang Doan , Thi Ngoc Tran , San Lang Wang , Anh Dzung Nguyen
{"title":"Enhancing the antibacterial activity of ampicillin loaded into chitosan/starch nanocomposites against AMR Staphylococcus aureus","authors":"Vinh Nghi Nguyen ,&nbsp;Van Bon Nguyen ,&nbsp;Minh Dinh Tran ,&nbsp;Manh Dung Doan ,&nbsp;Dinh Sy Nguyen ,&nbsp;Thi Huyen Nguyen ,&nbsp;Chien Thang Doan ,&nbsp;Thi Ngoc Tran ,&nbsp;San Lang Wang ,&nbsp;Anh Dzung Nguyen","doi":"10.1016/j.carres.2024.109274","DOIUrl":"10.1016/j.carres.2024.109274","url":null,"abstract":"<div><p>Ampicillin (Amp), an antibiotic, is widely used to treat bacterial infections in humans and livestock, but recently the rate of resistance has increased rapidly. The aim of this work was to enhancing the antibacterial effect of this compound against <em>AMR Staphylococcus aureus</em> via loading Amp into chitosan/starch nanocomposites by spray drying technique. The results showed that the different ratio of chitosan gel and starch gel used in preparing the nanocomposites can affect its properties and performance. The size distribution of the nanocomposite particles was ranging from 122.0 to 816.9 nm. The zeta potential values of the nanocomposites range from +29.47 to +93.07 mV, indicating the stability of the particles and their tendency to repel each other. Ampicillin was loaded into the chitosan/starch nanocomposites with encapsulation efficiency of 70.7–77.3 %, then their releasing and antibacterial effect against AMR <em>S. aureus</em> were investigated. The results indicated that antibacterial activity of chitosan/starch nanocomposites loaded ampicillin was much higher than ampicillin alone. Chitosan/starch nanocomposites loaded ampicillin at concentration 5.0 μg/mL inhibited 88.6 % growth of <em>S. aureus</em> to a similar extent as 7.5 μg/mL of ampicillin alone. Additionally, at same 7.5 μg/mL ampicillin concentration, the nanocomposites loaded ampicillin showed a higher inhibitory rate (93.27 %) compared to ampicillin alone (88.96 %) over a 12 h-period. Especially, the antibacterial activity of chitosan/starch nanocomposites loaded ampicillin still maintained their effectiveness over 48 h (95.43 %) while those the ampicillin decreased down to 85.76 %. This research highlights the potential of using the chitosan/starch nanocomposites as nanocarriers for ampicillin to enhance its antibacterial activity against AMR <em>Staphylococcus aureus.</em> This approach could be a promising strategy to combat antimicrobial resistance.</p></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"545 ","pages":"Article 109274"},"PeriodicalIF":2.4,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study on the role of alkali halides on the mutarotation and dehydration of d-xylose in aqueous solution 碱卤化物对水溶液中 d-木糖的变构和脱水作用的研究
IF 2.4 3区 化学
Carbohydrate Research Pub Date : 2024-09-12 DOI: 10.1016/j.carres.2024.109258
Zheng Li , Zhicheng Jiang , Yiping Luo , Chenyu Ge , Xiaoyan Wang , Changwei Hu
{"title":"Study on the role of alkali halides on the mutarotation and dehydration of d-xylose in aqueous solution","authors":"Zheng Li ,&nbsp;Zhicheng Jiang ,&nbsp;Yiping Luo ,&nbsp;Chenyu Ge ,&nbsp;Xiaoyan Wang ,&nbsp;Changwei Hu","doi":"10.1016/j.carres.2024.109258","DOIUrl":"10.1016/j.carres.2024.109258","url":null,"abstract":"<div><p>Although the xylose mutarotation and transformation have been investigated largely separately, their relationship has been rarely systematically elaborated. The effect of several factors such as xylose concentration, temperature, and salt concentration, affecting the mutarotation of xylose are discussed. Nine alkali halides (LiCl, NaCl, KCl, LiBr, NaBr, KBr, LiI, NaI, and KI) are used to test salt effects. The relationship between xylose rotation rate constant (<em>k</em><sub><em>M</em></sub>), specific optical rotation at equilibrium ([α]<sub>eqm</sub>), α/β ratio, H chemical shift difference (ΔΔδ), Gibbs free energy difference (ΔG), hydrogen ion or hydroxide ion concentration ([H<sup>+</sup>] or [OH<sup>−</sup>]), and xylose conversion is discussed. Different salts dissolved in water result in different pH of the solutions, which affect the mutarotation of xylose, with the nature of both cation and anion. Shortly, the smaller the cation radius is and the larger the anion radius is, the greater the mutarotation rate is. In the dehydration of xylose to furfural in salty solutions, xylose conversion is positively correlated to mutarotation rate, H<sup>+</sup> or OH<sup>−</sup> concentration, and the energy difference between α-xylopyranose and β-xylopyranose. Although the [α]<sub>eqm</sub> of xylose is positively correlated with α/β configuration ratio, there is no obvious correlation with xylose dehydration. The conversion to furfural in chlorides is superior to that in bromines and iodides, which is due to the fact that the pH of chloride salts is smaller than that of the corresponding bromide and iodized salts. Higher H<sup>+</sup> concentration prefers to accelerate the formation of furfural. In basic salt solutions, the xylulose selectivity is higher than that of furfural at the initial stage of reaction. The furfural selectivity and carbon balance are better in acidic condition rather than in basic condition. In H<sub>2</sub>O-MTHF (2-Methyltetrahydrofuran) biphasic system, the optimal furfural selectivity of 81.0 % is achieved at 190 °C in 1 h with the assistance of LiI and a little HCl (0.2 mmol, 8 mmol/L in aqueous phase). A high mutarotation rate represents rapid xylose conversion, but a high furfural selectivity prefers in acidic solutions, which would be perfect if organic solvents were available to form biphasic systems.</p></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"545 ","pages":"Article 109258"},"PeriodicalIF":2.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of ultrasonic degradation on physicochemical and antioxidant properties of Gleditsia sinensis seed polysaccharides 超声降解对皂荚多糖理化性质和抗氧化性的影响
IF 2.4 3区 化学
Carbohydrate Research Pub Date : 2024-09-12 DOI: 10.1016/j.carres.2024.109272
Qiuping Yao , Longlin Pu , Boyu Dong , Dequan Zhu , Wenwen Wu , Qiong Yang
{"title":"Effects of ultrasonic degradation on physicochemical and antioxidant properties of Gleditsia sinensis seed polysaccharides","authors":"Qiuping Yao ,&nbsp;Longlin Pu ,&nbsp;Boyu Dong ,&nbsp;Dequan Zhu ,&nbsp;Wenwen Wu ,&nbsp;Qiong Yang","doi":"10.1016/j.carres.2024.109272","DOIUrl":"10.1016/j.carres.2024.109272","url":null,"abstract":"<div><p>In this study, two degraded polysaccharides from <em>Gleditsia sinensis</em> seed were obtained under ultrasonic power treatments of 300 and 450 W. The physicochemical properties, structural characteristics, and antioxidant activities of the degraded and undegraded polysaccharides were studied and compared. Ion exchange chromatography and methylation analysis showed that the polysaccharides had similar basic structural features and were composed of the same monosaccharide units before and after degradation, but the ultrasonic treatment increased the total monosaccharide content and changed the Mannose/Galactose value. Furthermore, with the increase in the ultrasonic power, the molecular weight and intrinsic viscosity of polysaccharides decreased, and the micromorphology became looser. The scavenging capacities for 1,1-diphenyl-2-picrylhydrazyl and hydroxyl free radicals and the reducing ability were significantly increased by the ultrasonic treatment. In conclusion, ultrasonic treatment may be an effective way to improve the antioxidant activities of polysaccharides from <em>G</em>. <em>sinensis</em> seed, and further studies on its antioxidant mechanism are still needed.</p></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"545 ","pages":"Article 109272"},"PeriodicalIF":2.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the promising role of chitosan delivery systems in breast cancer treatment: A comprehensive review 探索壳聚糖给药系统在乳腺癌治疗中的前景:综述
IF 2.4 3区 化学
Carbohydrate Research Pub Date : 2024-09-07 DOI: 10.1016/j.carres.2024.109271
Jaya Lakkakula , G.K.P. Srilekha , Palak Kalra , S.A. Varshini , Suprasanna Penna
{"title":"Exploring the promising role of chitosan delivery systems in breast cancer treatment: A comprehensive review","authors":"Jaya Lakkakula ,&nbsp;G.K.P. Srilekha ,&nbsp;Palak Kalra ,&nbsp;S.A. Varshini ,&nbsp;Suprasanna Penna","doi":"10.1016/j.carres.2024.109271","DOIUrl":"10.1016/j.carres.2024.109271","url":null,"abstract":"<div><p>Breast cancer presents a significant global health challenge, driving the development of novel treatment strategies for therapeutic interventions. Nanotechnology has emerged as a promising avenue for addressing this challenge, with Chitosan (CS) nanoparticles receiving prominence due to their unique characteristics and multitude of potential applications. This review provides a comprehensive overview of the role of Chitosan nanoparticles in breast cancer therapy. The review begins by emphasizing the prevalence and importance of breast cancer as a major health issue, underscoring the necessity for effective treatments. It then delves into the application of Chitosan nanoparticles in breast cancer therapy. One key aspect discussed is their role as carriers for anticancer drugs, enabling targeted delivery and improved cellular uptake. Furthermore, the review explores modified Chitosan nanoparticles and strategies for enhancing their efficacy and specificity in breast cancer treatment. It also examines Chitosan conjugates and hybrids, which offer innovative approaches for combination therapy. Additionally, metal and magnetic Chitosan nanoparticles are discussed spanning their capacity to assist in imaging, hyperthermia, as well as targeted drug delivery. In conclusion, the review summarizes the current research landscape regarding Chitosan nanoparticles for breast cancer therapy and offers insights into future directions. Overall, the review highlights the versatility, potential benefits, and future prospects of Chitosan nanoparticles in combating breast cancer.</p></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"545 ","pages":"Article 109271"},"PeriodicalIF":2.4,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synthesis of a deuterated disaccharide internal standard for LC-MS/MS quantitation of heparan sulfate in biological samples 合成用于 LC-MS/MS 定量生物样本中硫酸肝素的氚代二糖内标物
IF 2.4 3区 化学
Carbohydrate Research Pub Date : 2024-09-07 DOI: 10.1016/j.carres.2024.109270
Yuchen Pan, Qi Qi He, Norbert Wimmer, Vito Ferro
{"title":"Synthesis of a deuterated disaccharide internal standard for LC-MS/MS quantitation of heparan sulfate in biological samples","authors":"Yuchen Pan,&nbsp;Qi Qi He,&nbsp;Norbert Wimmer,&nbsp;Vito Ferro","doi":"10.1016/j.carres.2024.109270","DOIUrl":"10.1016/j.carres.2024.109270","url":null,"abstract":"<div><p>High levels of heparan sulfate (HS) are a marker for several mucopolysaccharidosis (MPS) disorders which are lysosomal storage diseases caused by genetic defects in HS-degrading enzymes. Quantitation of HS in biological samples is therefore critical for diagnosis and evaluating the efficacy of new therapies. Herein we present the efficient synthesis of a butylated GlcN-GlcA disaccharide and its deuterated derivative for use as an internal standard in a quantitative mass spectrometry-based assay for analysis of HS following butanolysis. The synthesis features the stereoselective 1,2-<em>cis</em> glycosylation of a GlcA acceptor with a 6-<em>O</em>-benzoyl-2-deoxy-2-azido thioglucoside donor.</p></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"545 ","pages":"Article 109270"},"PeriodicalIF":2.4,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0008621524002490/pdfft?md5=b907954b03a55d5b604586d7cafdd595&pid=1-s2.0-S0008621524002490-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structure of the O-specific polysaccharide of Asaia bogorensis ATCC BAA-21 lipopolysaccharide Asaia bogorensis ATCC BAA-21 脂多糖的 O 型特异性多糖结构
IF 2.4 3区 化学
Carbohydrate Research Pub Date : 2024-09-05 DOI: 10.1016/j.carres.2024.109266
Anna Kaczmarek , Anna Maciejewska , Katarzyna Kasperkiewicz , Magdalena Noszczyńska , Jolanta Łukasiewicz
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