Journal of medical microbiology最新文献

{"title":"Clinical presentation and microbiological characteristics of community-acquired <i>Staphylococcus aureus</i> bacteraemia at a tertiary hospital in Costa Rica.","authors":"Natalia Solís, Cristian Pérez, Manuel Ramírez, José Castro, César Rodríguez","doi":"10.1099/jmm.0.001883","DOIUrl":"https://doi.org/10.1099/jmm.0.001883","url":null,"abstract":"<p><p><b>Introduction.</b> <i>Staphylococcus aureus</i> is a leading agent in community-acquired bacteraemia (CAB) and has been linked to elevated mortality rates and methicillin resistance in Costa Rica.<b>Gap statement and aim.</b> To update and enhance previous data obtained in this country, we analysed the clinical manifestations of 54 <i>S. aureus</i> CAB cases in a tertiary hospital and delineated the sequence types (STs), virulome, and resistome of the implicated isolates.<b>Methodology.</b> Clinical information was retrieved from patient files. Antibiotic susceptibility profiles were obtained with disc diffusion and automated phenotypic tests. Genomic data were exploited to type the isolates and for detection of resistance and virulence genes.<b>Results.</b> Primary infections predominantly manifested as bone and joint infections, followed by skin and soft tissue infections. Alarmingly, 70% of patients continued to exhibit positive haemocultures beyond 48 h of treatment modification, with nearly a quarter requiring mechanical ventilation or developing septic shock. The 30-day mortality rate reached an alarming 40%. More than 60% of the patients were found to have received suboptimal or inappropriate antibiotic treatment, and there was an alarming tendency towards the overuse of third-generation cephalosporins as empirical treatment. Laboratory tests indicated elevated creatinine levels, leukocytosis, and bandaemia within the first 24 h of hospitalization. However, most showed improvement after 48 h. The isolates were categorized into 13 STs, with a predominance of representatives from the clonal complexes CC72 (ST72), CC8 (ST8), CC5 (ST5, ST6), and CC1 (ST188). Twenty-four isolates tested positive for <i>mecA</i>, with ST72 strains accounting for 20. In addition, we detected genes conferring acquired resistance to aminoglycosides, MLS_B antibiotics, trimethoprim/sulfamethoxazole, and mutations for fluoroquinolone resistance in the isolate collection. Genes associated with biofilm formation, capsule synthesis, and exotoxin production were prevalent, in contrast to the infrequent detection of enterotoxins or exfoliative toxin genes.<b>Conclusions.</b> Our findings broaden our understanding of <i>S. aureus</i> infections in a largely understudied region and can enhance patient management and treatment strategies.</p>","PeriodicalId":94093,"journal":{"name":"Journal of medical microbiology","volume":"73 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing antimicrobial resistance profiles of <i>Salmonella enterica</i> in the pork production system.","authors":"Teerarat Prasertsee, Sakaoporn Prachantasena, Phakawat Tantitaveewattana, Podjanakorn Chuaythammakit, Ben Pascoe, Prapas Patchanee","doi":"10.1099/jmm.0.001894","DOIUrl":"https://doi.org/10.1099/jmm.0.001894","url":null,"abstract":"<p><p><b>Introduction.</b> <i>Salmonella enterica</i> is a significant enteric pathogen affecting human and livestock health. Pork production is a common source of <i>Salmonella</i> contamination, with emerging multidrug resistance (MDR) posing a global health threat.<b>Gap statement.</b> <i>Salmonella</i> contamination and antimicrobial resistance (AMR) profiles in the pig production chain are underreported.<b>Aim.</b> To investigate the prevalence of <i>S. enterica</i> in the pig production chain and characterise their AMR profiles.<b>Methodology.</b> We collected 485 samples from pig farms, a standard pig abattoir and retail markets in Patthalung and Songkhla provinces in southern Thailand. Antimicrobial susceptibility testing was performed on these samples, and AMR profiles were determined.<b>Results.</b> <i>S. enterica</i> was detected in 68.67% of farm samples, 45.95% of abattoir samples and 50.67% of retail market samples. Analysis of 264 isolates, representing 18 serotypes, identified <i>S. enterica</i> serotype Rissen as the most prevalent. The predominant resistance phenotypes included ampicillin (AMP, 91.29%), tetracycline (TET, 88.26%) and streptomycin (STR, 84.47%). Over 80% of isolates showed resistance to three or more antimicrobial classes, indicating MDR. The AMP-STR-TET resistance pattern was found in nearly 70% of all MDR isolates across the production chain.<b>Conclusions.</b> The high prevalence of MDR is consistent with extensive antimicrobial use in the livestock sector. The presence of extensively resistant <i>S. enterica</i> highlights the urgent need for antimicrobial stewardship. Strengthening preventive strategies and control measures is crucial to mitigate the risk of MDR <i>Salmonella</i> spreading from farm to fork.</p>","PeriodicalId":94093,"journal":{"name":"Journal of medical microbiology","volume":"73 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11423857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Launching the <i>Clostridioides difficile</i> collection.","authors":"Lovleen Tina Joshi, Stephen Michell, Ed Kuijper","doi":"10.1099/jmm.0.001877","DOIUrl":"10.1099/jmm.0.001877","url":null,"abstract":"","PeriodicalId":94093,"journal":{"name":"Journal of medical microbiology","volume":"73 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11335305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142006114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CagA 3' region polymorphism of <i>Helicobacter pylori</i> and its association with chronic gastritis in the Chinese population.","authors":"Xiaoyan Zhu, Chao Ma, Rina Sa, Yaxuan Wang, Chaohui Zhu, Yajiao Zhao, Juan Luo, Xiaochuan Liu","doi":"10.1099/jmm.0.001880","DOIUrl":"10.1099/jmm.0.001880","url":null,"abstract":"<p><p><b>Introduction</b>. Cytotoxin-associated gene A (CagA) from <i>Helicobacter pylori</i> is highly related to chronic gastritis. Tyrosine phosphorylation of Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs from CagA determines the pathogenicity of <i>H. pylori</i>.<b>Gap statement</b>. The precise amino acid variations surrounding the EPIYA motifs and their correlation with clinical outcomes have been poorly explored.<b>Aim</b>. The purpose of this study was to examine the CagA 3' region polymorphism of <i>H. pylori</i> and its association with chronic gastritis in the Chinese population.<b>Method</b>. A total of 86 <i>cagA</i>-positive <i>H. pylori</i> strains were isolated from patients with chronic gastritis in two different hospitals in Beijing, PR China. Genomic DNA was extracted commercial kits, and the <i>cagA</i> 3' variable region of <i>H. pylori</i> was amplified by polymerase chain reaction (PCR). The PCR products were sequenced and analysed using the CLC Sequence Viewer, BioEdit, and WebLogo 3.<b>Results</b>. Two hundred and fifty-nine EPIYA motifs were identified from <i>cagA</i>-positive <i>H. pylori</i> strains. Notably, EPIYA-B exhibited a higher frequency of variation in comparison to EPIYA-A, EPIYA-C, and EPIYA-D. The prevalent sequences for East-Asian-type CagA were QVNK and TIDF, while KVNK and TIDD were most commonly observed for Western-type CagA. The CRPIA motifs of East-Asian-type CagA and Western-type CagA varied at positions 4, 6, 7, 8, and 10. CagA-ABD (73.2%) was the most prevalent type, followed by CagA-ABC (18.6%) and CagA-AB (3.4%). The ratio of CagA-ABD was observed to be higher in cases of chronic non-atrophic gastritis with erosive (NAGE) or chronic atrophic gastritis (AG) compared to chronic non-atrophic gastritis (NAG), and the difference was found to be statistically significant (χ2=59.000/64.000, <i>P</i><0.001).<b>Conclusions</b>. The EPIYA segments of Western-type CagA and East-Asian-type CagA differ significantly and the presence of CagA-ABD may be associated with severe chronic gastritis from this study.</p>","PeriodicalId":94093,"journal":{"name":"Journal of medical microbiology","volume":"73 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marginal Notes, July 2024. Taken for Granted.","authors":"Timothy J J Inglis","doi":"10.1099/jmm.0.001876","DOIUrl":"10.1099/jmm.0.001876","url":null,"abstract":"","PeriodicalId":94093,"journal":{"name":"Journal of medical microbiology","volume":"73 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11335014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142006113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methicillin-resistant <i>Staphylococcus aureus</i> outbreak in a Dutch equine referral clinic.","authors":"Marleen M Kannekens-Jager, Birgitta Duim, Linda van der Graaf-van Bloois, Aldert L Zomer, Marian J Broekhuizen-Stins, Maarten Boswinkel, Jaap A Wagenaar, Els M Broens","doi":"10.1099/jmm.0.001873","DOIUrl":"10.1099/jmm.0.001873","url":null,"abstract":"<p><p>In 2020 and 2022, nine cases of surgical site infections with a methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) were diagnosed in horses in an equine referral clinic. Sixteen isolates (horses, <i>n</i>=9; environment, <i>n</i>=3; and staff members, <i>n</i>=4) were analysed retrospectively using Nanopore whole-genome sequencing to investigate the relatedness of two suspected MRSA outbreaks (2020 and 2022). The MRSA isolates belonged to ST398 and ST612. ST398 genomes from 2020 and 2022 formed three phylogenetic clusters. The first ST398 cluster from 2020 consisted of isolates from five horses and one staff member, and we suspected within clinic transmission. The second cluster of ST398 isolates from 2022 originated from two horses and two staff members but showed higher single nucleotide polymorphism (SNP) distances. One ST398 isolate from an individual staff member was not related to the other two clusters. The ST612 isolates were isolated in 2022 from two horses and three environmental samples and showed very low SNP distances (<7 SNPs), indicating the transmission of MRSA ST612 in this clinic in 2022. Molecular characterization revealed an abundant set of virulence genes and plasmids in the ST612 isolates in comparison to ST398 isolates. Phenotypic antimicrobial susceptibility showed that differences between the two sequence types were consistent with the genetic characteristics. MRSA ST612 has not been reported in Europe before, but it is a dominant clone in African hospitals and has been described in horses and people working with horses in Australia, indicating the importance of surveillance.</p>","PeriodicalId":94093,"journal":{"name":"Journal of medical microbiology","volume":"73 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11361372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142116596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining Gram stain and 16S qPCR improved diagnostic accuracy for suspected pneumonia and could become a new metric in the rapid diagnosis of lower respiratory tract infections.","authors":"Yunas Panikkaveettil Hamza, Mohamed Ali Ben Hadj Kacem, Naema Hassan Al Molawi, Hadi Mohamad Yassine, Hebah Atef Mohammad AlKhatib, Fatiha Benslimane, Hanan Ibrahim Kh B Al-Remaihi, Reham Awni El Kahlout, Basema Ibrahim Ahmed El Kahlout, Hajar Al Khalili, Makiyeh Ahmed Al Khalili, Sanjay H Doiphode, Emad Bashier Ibrahim Elmagboul, Javed Akhter, Einas A/Aziz Eid Al Kuwari, Peter V Coyle","doi":"10.1099/jmm.0.001861","DOIUrl":"https://doi.org/10.1099/jmm.0.001861","url":null,"abstract":"<p><p><b>Introduction.</b> The frequency of multidrug-resistant organisms (MDROs) in hospitals and the risk of delaying effective treatment result in the culture of respiratory secretions for nearly all patients with suspected pneumonia. Culture delays contribute to over prescribing and use of broader spectrum antibiotics.<b>Gap statement.</b> The need for improved rapid diagnostics for early assessment of suspected hospital pneumonia.<b>Aim.</b> To validate a new metric, enhanced Gram stain (EGS), to provide a rapid diagnostic test of high diagnostic accuracy that could be assessed in clinical trials of the use of antibiotics in suspected pneumonia.<b>Methodology.</b> Ninety-two residual lower respiratory samples previously tested by culture and Gram stain were re-tested by 16S ribosomal DNA real-time polymerase chain reaction (16S qPCR) and reported as a combined metric with Gram stain termed EGS. The EGS was assessed for diagnostic accuracy, standard performance measurements and correlation against culture. For samples with discordance between culture and EGS, 16S ribosomal DNA whole operon sequencing (16S rDNA WOS) was used for test resolution. An amended EGS (A-EGS was reassessed against culture.<b>Results.</b> Gram stain, 16S qPCR, EGS and A-EGS had respective diagnostic accuracies of 77.01 %, 82.76 %, 84.04 % and 94.19 %. The same platforms had respective correlation with culture of <i>r</i> = 0.67, <i>r</i> = 0.71, <i>r</i> = 0.81 and <i>r</i> = 0.89. EGS had the highest negative predictive value (NPV) of 93.18 % (81.99 %-97.62 %). Adding an 16S qPCR result is achievable in most routine laboratories and, combined with Gram stain, could improve early decision-making in patients with suspected hospital pneumonia.<b>Conclusion.</b> EGS could improve early decision-making in patients with suspected hospital pneumonia and could be assessed in clinical trials. The 16S rDNA WOS results in the A-EGS also supported the use of pathogen genomic sequencing in early decision making of suspected pneumonia.</p>","PeriodicalId":94093,"journal":{"name":"Journal of medical microbiology","volume":"73 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141984245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the impact of <i>Acinetobacter baumannii</i> on human health, and exploration of natural compounds as efflux pump inhibitors to treat multidrug resistance.","authors":"Karthiga Sivarajan, Ramya Ravindhiran, Jothi Nayaki Sekar, Rajeswari Murugesan, Kumarappan Chidambaram, Kavitha Dhandapani","doi":"10.1099/jmm.0.001867","DOIUrl":"https://doi.org/10.1099/jmm.0.001867","url":null,"abstract":"<p><p><i>Acinetobacter baumannii</i> is an ESKAPE pathogen and threatens human health by generating infections with high fatality rates. <i>A. baumannii</i> leads to a spectrum of infections such as skin and wound infections, endocarditis, meningitis pneumonia, septicaemia and urinary tract infections. Recently, strains of <i>A. baumannii</i> have emerged as multidrug-resistant (MDR), meaning they are resistant to at least three different classes of antibiotics. MDR development is primarily intensified by widespread antibiotic misuse and inadequate stewardship. The World Health Organization (WHO) declared <i>A. baumannii</i> a precarious MDR species. <i>A. baumannii</i> maintains the MDR phenotype via a diverse array of antimicrobial metabolite-hydrolysing enzymes, efflux of antibiotics, impermeability and antibiotic target modification, thereby complicating treatment. Hence, a deeper understanding of the resistance mechanisms employed by MDR <i>A. baumannii</i> can give possible approaches to treat antimicrobial resistance. Resistance-nodulation-cell division (RND) efflux pumps have been identified as the key contributors to MDR determinants, owing to their capacity to force a broad spectrum of chemical substances out of the bacterial cell. Though synthetic inhibitors have been reported previously, their efficacy and safety are of debate. As resistance-modifying agents, phytochemicals are ideal choices. These natural compounds could eliminate the bacteria or interact with pathogenicity events and reduce the bacteria's ability to evolve resistance. This review aims to highlight the mechanism behind the multidrug resistance in <i>A. baumannii</i> and elucidate the utility of natural compounds as efflux pump inhibitors to deal with the infections caused by <i>A. baumannii</i>.</p>","PeriodicalId":94093,"journal":{"name":"Journal of medical microbiology","volume":"73 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142116594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aspiration pneumonia after SARS-CoV-2 Omicron infection frequently induced physical functional decline in Japan.","authors":"Naoyuki Miyashita, Yasushi Nakamori, Makoto Ogata, Naoki Fukuda, Akihisa Yamura, Tomoki Ito","doi":"10.1099/jmm.0.001872","DOIUrl":"https://doi.org/10.1099/jmm.0.001872","url":null,"abstract":"<p><p><b>Introduction.</b> Nursing and healthcare-associated pneumonia (NHCAP) mainly occurs in older people whose physical functions have declined, and it is the most common type of pneumonia in Japan, a super-ageing society. In older people who meet NHCAP criteria, respiratory tract infections are often accompanied by aspiration pneumonia.<b>Gap statement.</b> The SARS-CoV-2 Omicron variant frequently causes aspiration pneumonia and has induced a decline in physical function.<b>Aim.</b> To clarify functional outcomes at 1 year after hospital discharge in SARS-CoV-2 Omicron-related NHCAP cases.<b>Methodology.</b> We compared the functional outcomes between 259 patients with primary SARS-CoV-2 pneumonia and 223 patients with aspiration pneumonia.<b>Results.</b> Functional decline rates for calculating the Barthel index at the time of hospital discharge were higher in the aspiration pneumonia group than the primary SARS-CoV-2 pneumonia group [114 patients (51.6%) vs 70 patients (27.0%), <i>P</i><0.0001]. Of 114 patients with aspiration pneumonia who had a decline in physical function at the time of hospital discharge, 91 (79.8%) still showed functional decline 1 year later. In contrast, 9.3% of patients had functional decline at 1 year after hospital discharge in the primary SARS-CoV-2 pneumonia group, which was significantly lower than in the aspiration pneumonia group.<b>Conclusions.</b> The Omicron variant showed decreased infectivity in the lungs and was less pathogenic compared with the Delta and former variants. However, physicians should recommend SARS-CoV-2 vaccination and non-pharmaceutical interventions, depending on the presence or absence of applicable criteria for NHCAP, even when the predominant strain is the Omicron variant.</p>","PeriodicalId":94093,"journal":{"name":"Journal of medical microbiology","volume":"73 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142116593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An end-point multiplex RT-PCR for SARS-CoV-2, Influenza A and B detection, including simultaneous RNAse P amplification: a timely tool for more accessible differential diagnosis.","authors":"Thaísa Regina Rocha Lopes, José Valter Joaquim Silva Júnior, Priscila de Arruda Trindade, Tatiana Schäffer Gregianini, Rudi Weiblen, Eduardo Furtado Flores","doi":"10.1099/jmm.0.001868","DOIUrl":"https://doi.org/10.1099/jmm.0.001868","url":null,"abstract":"<p><p>The multiplex molecular diagnostic assays described for severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), influenza A (IAV) and B (IBV) viruses have been mainly based on real-time reaction, which limits their access to many laboratories or diagnostic institutions. To contribute to available strategies and expand access to differential diagnosis, we describe an end-point multiplex RT-PCR targeting SARS-CoV-2, IAV and IBV with simultaneous endogenous control amplification. Initially, we looked for well-established primers sets for SARS-CoV-2, IAV, IBV and RNAse P whose amplicons could be distinguished on agarose gel. The multiplex assay was then standardized by optimizing the reaction mix and cycle conditions. The limit of detection (LoD) was determined using titrated viruses (for SARS-CoV-2 and IAV) and by dilution from a pool of IBV-positive samples. The diagnostic performance of the multiplex was evaluated by testing samples with different RNAse P and viral loads, previously identified as positive or negative for the target viruses. The amplicons of IAV (146 bp), SARS-CoV-2 (113 bp), IBV (103 bp) and RNAse P (65 bp) were adequately distinguished in our multiplex. The LoD for SARS-CoV-2, IAV and IBV was 0.02 TCID₅₀/ml, 0.07 TCID₅₀/ml and 10^-3 from a pool of positive samples, respectively. All samples positive for SARS-CoV-2 (<i>n</i>=70, Ct 17.2-36.9), IAV (<i>n</i>=53, Ct 14-34.9) and IBV (<i>n</i>=12, Ct 23.9-31.9) remained positive in our multiplex assay. RNAse P from negative samples (<i>n</i>=40, Ct 25.2-30.2) was also amplified in the multiplex. Overall, our assay is a timely and alternative tool for detecting SARS-CoV-2 and influenza viruses in laboratories with limited access to supplies/equipment.</p>","PeriodicalId":94093,"journal":{"name":"Journal of medical microbiology","volume":"73 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141977547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}