Christopher J Geden, Alex P Arp, Paul V Hickner, Mackenzie Tietjen, Alexandra A Pagac
{"title":"Susceptibility of secondary screwworm (Diptera: Calliphoridae) to the larval parasitoid Tachinaephagus zealandicus (Hymenoptera: Encyrtidae).","authors":"Christopher J Geden, Alex P Arp, Paul V Hickner, Mackenzie Tietjen, Alexandra A Pagac","doi":"10.1093/jme/tjag087","DOIUrl":"10.1093/jme/tjag087","url":null,"abstract":"<p><p>The reinfestation of New World screwworm (Cochliomyia hominivorax [Coquerel]) (Diptera: Calliphoridae) in North America has prompted a broad effort to identify new management tools. In this study, the larval parasitoid Tachinaephagus zealandicus (Ashmead) (Hymenoptera: Encyrtidae) was evaluated as a potential biocontrol using the congeneric secondary screwworm Cochliomyia macellaria (Fab.) (Diptera: Calliphoridae) as a proxy host. When host:parasitoid ratios were varied from 5:1 to 40:1, female parasitoids killed up to 18 hosts (at 40:1) and produced 66 adult wasp progeny per female over 24 h. Parasitized pupae produced from 5.6 to 11.7 adult parasitoids per pupa at host:parasitoid ratios of 40:1 and 5:1, respectively. Parasitoids showed no preference for larvae of C. macellaria or Sarcophaga bullata Parker (Diptera: Sarcophagidae). Kill rates and successful parasitism was twice as high in moist (15% moisture) as in dry sandy loam soil. Addition of a layer of leaf litter to the soil surface had no effect on the ability of parasitoids to locate hosts. Parasitism in moist clay soil was similar to that in moist sandy soil, but higher parasitism was observed in dry clay than on dry sandy soil. Placing faux rocks as potential larval refuge on the pupation substrate surface provided larvae with some protection from parasitism in dry substrates. When larvae were given a 2-h head start before adding parasitoids, there was no effect on host mortality or parasitism in dry soil. Results are encouraging for possible use of T. zealandicus as a supplemental management tool for calliphorid pests.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148207273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Chlorella sorokiniana KU.B2 microalga on the development of Culex quinquefasciatus (Diptera: Culicidae) larvae.","authors":"Jemisha Dudhat, Jassada Saingamsook, Baby Kyi Soe, Nuttha Sanevas, Saowalak Kaewmee, Chonlada Mano, Catherine Walton, Padet Siriyasatien, George Dimopoulos, Narissara Jariyapan","doi":"10.1093/jme/tjag089","DOIUrl":"https://doi.org/10.1093/jme/tjag089","url":null,"abstract":"<p><p>Culex quinquefasciatus (Say) is a significant vector of lymphatic filariasis and arboviruses. Current control strategies rely heavily on chemical insecticides, highlighting the need for alternative and environmentally sustainable approaches. This study evaluated the effects of the microalga Chlorella sorokiniana KU.B2 on the development of Cx. quinquefasciatus larvae. Larvae were reared on microalgal-rabbit food diets: a control (100% rabbit food) and four treatments with increasing proportions of C. sorokiniana suspension (25 microalgae [Al], 50Al, 75Al, and 100Al). Additional rabbit food-only controls with matched food proportions were included to distinguish microalgal effects from reduced food availability. Larval growth, pupation, adult emergence, and mortality were assessed, and gut histology and ultrastructure were examined descriptively. Higher proportions of microalgae (75Al and 100Al) were associated with failure of pupation and adult emergence, resulting in complete larval mortality. Lower proportions (25Al and 50Al) caused delayed development and reduced survival compared with controls. Larvae provided reduced amounts of rabbit food alone were still able to complete development, indicating that the observed effects were associated with microalgal inclusion rather than reduced food availability alone. Histological and ultrastructural observations revealed numerous intact and partially degraded microalgal cells within the larval gut, while recovery of viable cells after gut passage suggested that some ingested cells remained viable after transit through the larval digestive tract. Overall, C. sorokiniana KU.B2 negatively affected the development and survival of Cx. quinquefasciatus larvae in a dose-dependent manner. Further investigation into the mechanisms underlying these effects and their ecological relevance is needed.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148255297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multiple genetic lineages of the invasive brown widow spider (Araneae: Theridiidae) from inland areas in the Kanto region, Japan.","authors":"Hirotaka Katahira, Syu Tsutsui, Sana Kishino, Takumi Tsujino, Hiroki Yajima, Yuusuke Sawada, Yuya Eguchi, Takahiro Horiguchi, Rei Hirasawa, Daisuke Hayasaka, Kozue Nitta","doi":"10.1093/jme/tjag077","DOIUrl":"10.1093/jme/tjag077","url":null,"abstract":"<p><p>The brown widow spider, Latrodectus geometricus Koch, 1841, is a medically important invasive species that possesses neurotoxic venom capable of causing latrodectism in humans. This species has recently been found in inland areas of the Kanto region in Japan, raising concerns about its ongoing range expansion and associated public health risks. Here, we analyzed partial sequences of mitochondrial cytochrome c oxidase subunit I (cox1) and 16S ribosomal DNA from 99 individuals (3 males and 96 females) collected from multiple sites in Tokyo and Kanagawa prefectures, evaluating their phylogenetic positions and introduction history. The analysis revealed two major lineages, with six cox1 haplotypes (LGc1-LGc6) and five 16S rDNA haplotypes (LGs1-LGs5). One lineage comprised previously unreported basal haplotypes, including LGc5 and LGc6 in cox1 and LGs5 in 16S rDNA. The other lineage included four haplotypes (LGc1-LGc4 and LGs1-LGs4), which have been reported from South Africa and various invaded regions globally. In Sagamihara City (Kanagawa), where numerous individuals were consistently detected in previous surveys, both lineages co-occurred, suggesting multiple introductions and/or high propagule pressure. To clarify the dispersal routes and source populations of each lineage and haplotype, more comprehensive genetic data from both its native range in Africa and globally established populations are needed.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148177245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Spotted lanternfly honeydew as a sugar source for mosquitoes.","authors":"Irving Forde Upshur, Chin-Cheng Scotty Yang, Chloé Lahondère","doi":"10.1093/jme/tjag086","DOIUrl":"https://doi.org/10.1093/jme/tjag086","url":null,"abstract":"<p><p>Spotted lanternflies and mosquitoes are among the most concerning invasive insects, which have introduced a myriad of public health, social, economic, and agricultural problems. In addition to their characteristic blood-feeding behavior, mosquitoes exhibit frequent sugar-feeding activity, targeting a diverse range of sugar sources. Spotted lanternflies are ravenous sap-feeders and, as a result, produce large aggregates of honeydew, a sugary excreta. Here, we used spotted lanternfly honeydew-specific DNA barcoding to determine that both invasive and native mosquito species feed on spotted lanternfly honeydew in the field. The implications and applications of these findings for invasive interspecies interactions and disease vector management are discussed.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148240888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lynn M Osikowicz, Andrias Hojgaard, Mason Yonge, Shelby L Ford, Erik Foster, Sarah E Maes, Rebecca J Eisen
{"title":"Geographic variation in the distribution of hard tick relapsing fever spirochete (Borrelia miyamotoi) (Spirochaetales: Spirochaetaceae) genotypes in host-seeking Ixodes scapularis (Acari: Ixodidae) nymphs and adults in the eastern United States.","authors":"Lynn M Osikowicz, Andrias Hojgaard, Mason Yonge, Shelby L Ford, Erik Foster, Sarah E Maes, Rebecca J Eisen","doi":"10.1093/jme/tjag104","DOIUrl":"https://doi.org/10.1093/jme/tjag104","url":null,"abstract":"<p><p>Borrelia miyamotoi Fukunaga (Spirochaetales: Spirochaetaceae), the etiological agent of hard tick relapsing fever, has been detected at low prevalence in Ixodes spp. (Acari: Ixodidae) ticks from the Northern Hemisphere. Genetically distinct populations of B. miyamotoi have been described across continents and among Ixodes tick species distributed within continents. Within the eastern United States, a previous study identified two B. miyamotoi genotypes infecting Ixodes scapularis Say (Acari: Ixodidae). Here, we expand upon that work by developing a new multiplex PCR amplicon sequencing assay to differentiate B. miyamotoi genotypes (Am-East-1 and Am-East-2), and we use the new assay to differentiate genotypes in an expanded sample of 186 B. miyamotoi ticks collected from 17 eastern states. All 80 infections derived from the Northeast (Delaware, Massachusetts, Maryland, Maine, New Hampshire, and Vermont) or northern states in the Southeast region (North Carolina and Virginia) were identified as Am-East-1. Among the 106 B. miyamotoi infections identified in I. scapularis from the Ohio Valley (Illinois, Indiana, Kentucky, Ohio, and West Virginia), Upper Midwest (Michigan, Minnesota, and Wisconsin), or Northern Rocky Mountains and Plains (Nebraska) regions, 10 (9.4%) were characterized as Am-East-1 and 96 (90.6%) were characterized as Am-East-2. We also identified a new B. miyamotoi genotype (Am-IxKi1) in a single I. keiransi Beati, Nava, Venzal, & Guglielmone (Acari: Ixodidae) collected from Virginia.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agustín Maceyra, Laura V Harburguer, Ariel C Toloza, Paula V Gonzalez
{"title":"Chain length influences the repellency of natural aliphatic lactones against Aedes aegypti (Diptera: Culicidae).","authors":"Agustín Maceyra, Laura V Harburguer, Ariel C Toloza, Paula V Gonzalez","doi":"10.1093/jme/tjag073","DOIUrl":"10.1093/jme/tjag073","url":null,"abstract":"<p><p>Aedes aegypti (L.) is a widely distributed mosquito and the primary vector of arboviruses such as dengue. Its obligatory hematophagy, high environmental adaptability, and close association with humans make it a major public health challenge. Natural repellents with low environmental impact are currently being evaluated in experiments as potential complementary tools for managing Ae. aegypti. The development of environmentally compatible repellents has become a priority in vector control strategies. Lactones are cyclic esters naturally present in various foods and fragrances and have been associated with insect behavioral modulation. Based on these properties, we studied the repellency of natural aliphatic lactones against female Ae. aegypti. Repellency was evaluated using a modified tube-choice bioassay. Lactones differing in side-chain length were tested at 7% and compared with N, N-diethyl-m-toluamide (DEET, 7%) and ethanol as a negative control. Repellency efficacy (ER) was quantified at 5, 10, 30, and 60 min post-exposure. Lactones with 11 and 12 carbons showed little or no repellency (ER60min: 37.6 and 19.5, respectively), whereas γ-hexalactone (C6), γ-octalactone (C8), and γ-nonalactone (C9) achieved ER60min values of 89.7, 83.7, and 74.3, comparable or superior to DEET (ER60min: 72.7). A significant negative correlation was observed between chain length and behavioral repellency (mosquito avoidance). Finding efficient natural repellents is crucial for personal protection plans, especially in areas of the Americas where dengue and other arboviral diseases are expanding.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148038721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chip F Markwardt, Cassandra Olds, Phillip Shults, Bethany L McGregor
{"title":"Natural sugar-source associations of Culicoides biting midges (Diptera: Ceratopogonidae) in a Kansas tallgrass prairie.","authors":"Chip F Markwardt, Cassandra Olds, Phillip Shults, Bethany L McGregor","doi":"10.1093/jme/tjag065","DOIUrl":"10.1093/jme/tjag065","url":null,"abstract":"<p><p>Culicoides Latreille is a diverse genus of approximately 1,300 species of small biting flies found across the globe, containing numerous species of medical and veterinary importance. Many diseases associated with Culicoides have no specific treatment or vaccine, necessitating prevention through vector control. Current surveillance and control methods are limited by a lack of ecological knowledge about most Culicoides species, including phytophagous (sugar-feeding) behavior. The plants fed on by wild Culicoides are largely unknown, as are the driving forces behind selection of specific plants. To determine which plants midges are sugar-feeding on in nature, Culicoides were collected from five sites within a tallgrass prairie ecosystem in northeastern Kansas. Molecular sugar meal analysis was performed to detect plant DNA within field-collected midges. DNA was extracted from a total of 788 biting midge individuals, with 321 (40.7%) of these successfully amplifying and just 78 (9.9%) producing a sequence with a successful host match. Most matches were only identified to the plant family level with high confidence. Culicoides were found to feed on a diverse assemblage of families, including the Amaranthaceae, Cupressaceae, Solanaceae, and Verbenaceae; however, no significant association was found between Culicoides species and plant family. These results suggest that multiple sugar sources, including plant nectar and fruit, are utilized by biting midges under natural conditions. This information is vital for improving ecologically based control strategies, such as attractive toxic sugar baits, that can reduce disease burden on livestock and wildlife.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147847995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Michael J Skvarla, Karen C Poh, Jesse R Evans, Erika Machtinger
{"title":"Mapping European deer ked (Diptera: Hippoboscidae) abundance across Pennsylvania using traditional and citizen science methods.","authors":"Michael J Skvarla, Karen C Poh, Jesse R Evans, Erika Machtinger","doi":"10.1093/jme/tjag074","DOIUrl":"10.1093/jme/tjag074","url":null,"abstract":"<p><p>European deer keds (Diptera: Hippoboscidae: Lipoptena cervi (Linnaeus, 1758)) are blood-feeding ectoparasitic flies that usually feed on deer and other cervids but sometimes bite humans. Introduced into North America in the late 1800s, they have since become widespread in the Northeast. European deer keds were historically reported at the regional or state level, but recent work has begun to clarify their range by compiling county-level occurrence data. We expand upon this trend by using multiple methods to determine where deer keds are relatively more and less abundant in Pennsylvania. Based on our results, European deer keds are most abundant in northern Pennsylvania, present but less abundant in central and western Pennsylvania, and absent or nearly so from southeastern counties. Understanding where deer keds occur will inform public education and allow the public to better protect themselves from deer ked bites.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148159466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Haemosporidian parasites in Kansas birds: molecular and histological evidence implicating Culicoides haematopotus (Diptera: Ceratopogonidae) in transmission.","authors":"Ilya Verekhman, Amanda Fideldy, Caitlin Taylor, Alec Fehr, Bethany McGregor, Phillip Shults","doi":"10.1093/jme/tjag098","DOIUrl":"10.1093/jme/tjag098","url":null,"abstract":"<p><p>Haemosporidian parasites of birds are maintained through complex interactions among vertebrate hosts, arthropod vectors, and the parasites themselves, yet their vector-parasite relationships remain poorly resolved under natural conditions in North America. Haemoproteus species are among the most prevalent avian blood parasites and have been implicated in reduced host fitness and malaria-like disease. Here, we combined molecular and histological approaches to investigate Haemoproteus prevalence in wild birds and to identify potential Culicoides biting midge vectors in Kansas. Blood meals from field-collected Culicoides and hunter-collected turkey blood samples were screened using nested PCR targeting the Haemoproteus cytochrome b gene, followed by Sanger sequencing. We additionally dissected and microscopically examined salivary glands as well as turkey blood smears. Nearly all turkey blood samples (95.7%) were positive for Haemoproteus, revealing 2 distinct parasite clades and frequent coinfections. In contrast, Culicoides-based surveillance may have underestimated parasite prevalence in turkeys. Only Culicoides haematopotus consistently tested positive by PCR, and this species was the only one in which Haemoproteus sporozoites were detected in salivary glands. Sequencing of PCR amplicons from unfed C. haematopotus revealed amplification of a Culicoides-specific noncoding genomic region, highlighting limitations of molecular detection alone for vector incrimination. Together, these findings provide field-based evidence that C. haematopotus may serve as a vector of Haemoproteus in wild turkeys in Kansas, emphasizing the importance of integrating molecular and histological data when evaluating vector competence and potential population-level effects.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13310037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lisset Roblejo-Arias, Adrian A Diaz-Sánchez, Roxana Marrero-Perera, Evelyn Lobo-Rivero, Belkis Corona-González, Neil B Chilton
{"title":"Genetic diversity of Dermacentor nitens (Acari: Ixodidae) collected on horses from western Cuba.","authors":"Lisset Roblejo-Arias, Adrian A Diaz-Sánchez, Roxana Marrero-Perera, Evelyn Lobo-Rivero, Belkis Corona-González, Neil B Chilton","doi":"10.1093/jme/tjag059","DOIUrl":"10.1093/jme/tjag059","url":null,"abstract":"<p><p>The tropical horse tick, Dermacentor nitens Neumann, 1897, has a wide distribution throughout the Neotropics, and is a vector of protozoan haemoparasites that cause equine piroplasmosis. Despite this, little is known about the genetic diversity of this tick species, particularly in Cuba, where horses are important domestic animals used in agriculture and for transportation. The objective of this study was to assess the magnitude of genetic variation in 115 D. nitens adults (57 females and 58 males) collected from horses in the Cuban province of Mayabeque using sequence data of two mitochondrial genes, cytochrome c oxidase subunit 1 gene (cox1) and NADH dehydrogenase subunit 4 gene (nad4). The results revealed the presence of 7 cox1 haplotypes, 14 nad4 haplotypes, and 23 haplotypes with concatenation of the two genetic markers. However, the results of the rarefaction curve analyses predict the existence of other rare haplotypes in Mayabeque Province. The most common haplotype for each genetic marker was identical to a D. nitens from Brazil, Colombia, Panama, and French Guiana. The star-like haplotype networks, each with a central (ie \"ancestral\") and most abundant haplotype, combined with the significant negative values for the Tajima's D and Fu's F tests, is consistent with a hypothesis of the introduction of D. nitens on horses from mainland South America (ie a founder effect) followed by demographic expansion. Additional population genetic studies on D. nitens are needed in other countries where this species occurs to examine the phylogeography of this tick species of socioeconomic importance.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13180250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147960395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}