Maher Ramadan I Alhawsawi, David A Lewis, Ronja A Frigard, Ellen M Smith, Jaishna Sivakumar, Ajay M Sharma, Adalynn R Nantz, Chloe Elizabeth G Sabile, Jasmine Kennedy, Rashi Loni, Gabrielle LeFevre, Akshita Vaka, Quinn Leanza, Melissa Kelley, Crystal L Stacey, Richa A Santhosh, Nathan Catlett, Tabitha L Cady, Raaidh S Rizvi, Zach Wagner, Pia U Olafson, Joshua B Benoit
{"title":"Developmental stage and level of submersion in water impact the viability of lone star and winter tick eggs.","authors":"Maher Ramadan I Alhawsawi, David A Lewis, Ronja A Frigard, Ellen M Smith, Jaishna Sivakumar, Ajay M Sharma, Adalynn R Nantz, Chloe Elizabeth G Sabile, Jasmine Kennedy, Rashi Loni, Gabrielle LeFevre, Akshita Vaka, Quinn Leanza, Melissa Kelley, Crystal L Stacey, Richa A Santhosh, Nathan Catlett, Tabitha L Cady, Raaidh S Rizvi, Zach Wagner, Pia U Olafson, Joshua B Benoit","doi":"10.1093/jme/tjae143","DOIUrl":"10.1093/jme/tjae143","url":null,"abstract":"<p><p>Female ticks deposit large egg clusters that range in size from hundreds to thousands. These egg clusters are restricted to a deposition site as they are stationary, usually under leaf litter and other debris. In some habitats, these sites can be exposed to periodic flooding. When the clusters of tick eggs are disturbed, they may float to the surface or remain underneath organic debris entirely submerged underwater. Here, we examined the viability of egg clusters from winter ticks, Dermacentor albipictus, and lone star ticks, Amblyomma americanum, when partially or fully submerged in water and in relation to the developmental stages of the eggs under lab conditions. In general, egg clusters that were older and partially submerged had a higher viability than fully submerged, younger eggs. Of the two species, A. americanum was more resistant to water exposure. These studies highlight that egg clusters for certain tick species can remain viable when exposed to water for at least two weeks. These results also suggest that distribution by flooding of egg clusters could occur for some species and water submersion will differentially impact tick egg survival based on the specific developmental stage of exposure and species.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"479-484"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammad Bagher Erfanian, Hossein Barahoei, Mohammad Mahdi Zeynali, Omid Mirshamsi
{"title":"Employing habitat suitability modeling to assess the distribution and envenomation potential of scorpion species in Iran.","authors":"Mohammad Bagher Erfanian, Hossein Barahoei, Mohammad Mahdi Zeynali, Omid Mirshamsi","doi":"10.1093/jme/tjae151","DOIUrl":"10.1093/jme/tjae151","url":null,"abstract":"<p><p>Iran is a biodiversity hotspot of scorpions with 80 recorded species. Thus, scorpion envenomation is a serious public health problem in the country. Here, we used habitat suitability modeling to assess the spatial distribution of scorpions in Iran. Only 45 species had sufficient georeferenced data. We used bioclimatic variables, soil temperature layers, and 9 modeling algorithms to perform habitat suitability modeling. We employed an ensemble approach to obtain the final models. We calculated the richness map and drew distribution maps for genera with more than 1 species. Also, we assessed the scorpions' species richness inside and outside of national parks. Finally, we created a risk map of encountering a venomous scorpion. The results showed that the highest scorpion richness is in the southern and southwestern parts of Iran, especially in the coastal areas of the Persian Gulf. We observed 3 biodiversity hotspots for scorpions that are located in the south and southwestern, central parts, and eastern parts of Iran. Except for northern parts, there is a possibility of encountering a venomous scorpion in other parts of Iran. The 3 biodiversity hotspots are also the areas with the highest chance of encountering a venomous species. We found that the hotspots are not protected and are located in areas facing land-use changes. Thus, hotspots have the highest human-scorpion conflicts. Our results provide new insight into the distribution of scorpion species in Iran. Conservation actions that ensure both human safety and species richness are essential and can be achieved by halting further land degradation in scorpion habitats and providing easy-to-understand manuals for local people.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"337-346"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142901472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Christina M Parise, Shelby L Ford, James Burtis, Andrias Hojgaard, Rebecca J Eisen, Lars Eisen
{"title":"Acquisition of Borrelia burgdorferi sensu stricto (Spirochaetales: Spirochaetaceae) by Haemaphysalis longicornis (Acari: Ixodidae) nymphs during interrupted feeding.","authors":"Christina M Parise, Shelby L Ford, James Burtis, Andrias Hojgaard, Rebecca J Eisen, Lars Eisen","doi":"10.1093/jme/tjae156","DOIUrl":"10.1093/jme/tjae156","url":null,"abstract":"<p><p>A previous laboratory study using Haemaphysalis longicornis Neumann (Acari: Ixodidae) ticks of North American origin showed that larvae could acquire the Lyme disease spirochete, Borrelia burgdorferi sensu stricto (s.s.) (Spirochaetales: Spirochaetaceae) while feeding to completion on infected mice. However, the infection was lost during the molt to the nymphal stage. Nonetheless, questing H. longicornis nymphs and adults collected by drag sampling in the northeastern United States have been reported infected with B. burgdorferi s.s. DNA; occasionally these ticks appeared to be partially engorged. This raises the question of whether H. longicornis ticks can (i) acquire B. burgdorferi s.s. during an interrupted, partial blood meal on an infected host and (ii) transmit spirochetes while completing the blood meal on a second host. In this laboratory study, we demonstrated that H. longicornis nymphs could acquire B. burgdorferi s.s. from infected Mus musculus mice during a partial blood meal. Borrelia burgdorferi s.s. was detected by a multiplex polymerase chain reaction amplicon sequencing assay in 2 of 32 (6.3%) nymphs allowed to remain attached to infected mice for 48 h but, paradoxically, not in any of 25 nymphs that remained attached to infected mice for 72 h. Unfortunately, due to the low percentage of infected nymphs, we were not able to examine if such partially fed, infected nymphs were able to transmit B. burgdorferi s.s. while completing their blood meal on a second, naïve host.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"475-478"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marie Paul Audrey Mayi, Cyril Kowo, Foncha David Forfuet, Damian Nota Anong, Andongma Esack Fonda, Mirabel Elad, Charlène Jordane Piam Djomo, Timoleon Tchuinkam, Ravinder N M Sehgal, Anthony John Cornel
{"title":"Water sources selected for immature development of some African rainforest dwelling mosquitoes under different landscapes in Cameroon.","authors":"Marie Paul Audrey Mayi, Cyril Kowo, Foncha David Forfuet, Damian Nota Anong, Andongma Esack Fonda, Mirabel Elad, Charlène Jordane Piam Djomo, Timoleon Tchuinkam, Ravinder N M Sehgal, Anthony John Cornel","doi":"10.1093/jme/tjae146","DOIUrl":"10.1093/jme/tjae146","url":null,"abstract":"<p><p>Little is known about the behaviors of African equatorial rain forest mosquito species and their potential role as sylvatic and bridge-vectors of various pathogens of animal and public health. In 2016 and 2017, the diversity and sources of water supporting immature development of mosquitoes in Talangaye Rainforest (South West Cameroon) before, during and after deforestation were investigated. Mosquito eggs, larvae and pupae were collected from 12 natural, seminatural, and artificial water sources and reared to adults. A total of 595 adult mosquitoes belonging to seven genera and at least 43 species were identified. Culex was the most abundant (56.3%) and was encountered in the majority in bamboo pots. Aedes and Uranotaenia species were mostly found in rock pools, while Anopheles and Hodgesia species solely prefer stream pools. In terms of mosquito abundance, rock pools were the most productive (29.91%) followed by bamboo pots (24.7%). Natural sites such as rock pools, tree holes, and stream pools recorded a greater number of species (S = 21, 14 and 12 respectively). During the rainy season, rock pools (46.23%) and bamboo pots (18.7%) were the most productive water bodies, while in the dry season, bamboo pots (35.71%) and stream pools (35.71%) harbored the most mosquitoes. The disturbed and pristine-like habitats had the greatest number of mosquitoes and breeding sites compared to palm plantation. This study provides some useful data on water sources used for immature development of forest mosquito species in Southwest Cameroon and how some species might adapt to changing landscapes, especially due to deforestation.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"301-314"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zephirin Somda, Nicolas Zanré, Dimitri W Wangrawa, Hyacinthe K Toé, Aboubacar Sombié, Erisha Saiki, Shinya Fukumoto, Tatsuya Sakurai, Antoine Sanon, Philip J McCall, Hirotaka Kanuka, David Weetman, Athanase Badolo
{"title":"High pyrethroid resistance is associated with high frequencies of 1014F and 1014S kdr mutations in Anopheles arabiensis (Diptera: Culicidae) from Ouagadougou, Burkina Faso.","authors":"Zephirin Somda, Nicolas Zanré, Dimitri W Wangrawa, Hyacinthe K Toé, Aboubacar Sombié, Erisha Saiki, Shinya Fukumoto, Tatsuya Sakurai, Antoine Sanon, Philip J McCall, Hirotaka Kanuka, David Weetman, Athanase Badolo","doi":"10.1093/jme/tjae135","DOIUrl":"10.1093/jme/tjae135","url":null,"abstract":"<p><p>Malaria remains a major public health threat in Burkina Faso, as in most sub-Saharan Africa countries. Malaria control relies mainly on long-lasting insecticide-treated nets (LLINs) and indoor residual spraying. In Burkina Faso, an escalating of insecticide resistance has been observed over the last decades. This study aimed to investigate insecticide resistance and the underlying mechanisms in Anopheles gambiae complex in Ouagadougou. Anopheles gambiae s.l. larvae were collected from gutters and ponds, in Zogona, Tampouy and Tanghin, 3 localities in Ouagadougou from July to October 2018. The larvae were reared in the laboratory to adults stage and susceptibility profile to pyrethroid, carbamate, and organophosphate insecticides was assessed using WHO tube assays. Mosquito species and mutations linked with insecticide resistance, were identified through PCR. More than 95% of the collected An. gambiae s.l. were An. arabiensis. An. arabiensis displayed high resistance to permethrin and deltamethrin, with mortalities below 30%, but was fully susceptible to bendiocarb, fenitrothion, and malathion. A high-frequency of the pyrethroid resistance-associated kdr mutation 1014F (0.81) was recorded, while the frequency of 1014S mutation (0.18) was lower. However, the carbamate and organophosphate-associated Ace-1 119S mutation was not detected. Localities and breeding site type appear to influence pyrethroid resistance in the An. arabiensis population of Ouagadougou. The high resistance to pyrethroids in An. arabiensis of urban Ouagadougou is underpinned, at least in part by high-frequency kdr mutations. This result supports the switch to next-generation LLINs, in well-established pyrethroid resistance zones of Burkina Faso including Ouagadougout.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"381-388"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vincent Mai, Robert A Boria, Kerry Padgett, Michelle S Koo, Megan E M Saunders, Sarah Billeter, Javier Asin, Savannah Shooter, Maria Zambrano, Sandor Karpathy, Christopher D Paddock, Andrea Swei
{"title":"Molecular and niche modeling approaches to identify potential amplifying hosts for an emerging tick-borne pathogen, Rickettsia rickettsii subsp. californica, the causative agent of Pacific Coast tick fever.","authors":"Vincent Mai, Robert A Boria, Kerry Padgett, Michelle S Koo, Megan E M Saunders, Sarah Billeter, Javier Asin, Savannah Shooter, Maria Zambrano, Sandor Karpathy, Christopher D Paddock, Andrea Swei","doi":"10.1093/jme/tjae147","DOIUrl":"10.1093/jme/tjae147","url":null,"abstract":"<p><p>Pacific Coast tick fever is a recently described zoonotic disease in California caused by a spotted fever group rickettsia, Rickettsia rickettsii subsp. californica (formerly Rickettsia 364D) and transmitted by the Pacific Coast tick, Dermacentor occidentalis. Like many emerging vector-borne diseases, knowledge regarding the transmission cycle, contribution from potential amplifying hosts, and geographic distribution of R. rickettsii californica is limited. We paired molecular analysis with comparative spatial niche modeling to identify vertebrate hosts potentially involved in the transmission cycle of this pathogen. We identified R. rickettsii californica DNA in three mammal species (Otospermophilus beecheyi, Lepus californicus, and Sylvilagus audubonii). This is the first record of R. rickettsii californica detected in mammals and may indicate potential amplifying hosts for this human pathogen. Species niche modeling of uninfected and infected D. occidentalis identified areas of high suitability along the coast and Sierra Nevada foothills of California. These findings support the hypothesis that amplifying host(s) may support higher infection prevalence in the infected tick regions compared to other parts of the tick's range. Potential host species distribution models (SDMs) were constructed from museum records and niche overlap statistics were used to compare habitat suitability with R. rickettsii californica-infected tick SDMs. We found higher than null overlap of infected ticks with California ground squirrels (O. beecheyii) and trending, but nonsignificant, overlap with two lagomorph species. Pairing molecular and niche modeling may be a useful approach to identify species that are involved in the maintenance of emerging tick-borne zoonoses.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"399-408"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Áine Lehane, Emily M Mader, Joseph D Poggi, Kaci D McCoy, Jeff Gruntmeir, Caroline T Weldon
{"title":"A scoping review of applied tick control research in North America: funding, implementation, and advancement.","authors":"Áine Lehane, Emily M Mader, Joseph D Poggi, Kaci D McCoy, Jeff Gruntmeir, Caroline T Weldon","doi":"10.1093/jme/tjae155","DOIUrl":"10.1093/jme/tjae155","url":null,"abstract":"<p><p>Geographic ranges of ticks and tick-borne pathogens within North America are shifting due to environmental changes and human-driven activities, with species of public health concern presenting a multifaceted risk to human health. Innovative strategies and continued collaboration to control tick populations are needed to combat this growing threat. We conducted a scoping review of the literature to describe the nature of applied tick control research conducted in North America (Canada, Mexico, and the United States) to date, with the goal of describing key concepts and identifying gaps in this research area. A total of 244 articles met our inclusion criteria and were reviewed for patterns in applied tick control authorship and funding, study location, target species, and control methodology. Most studies (83.6%) were conducted exclusively in the United States and 75% focused on ticks of public health concern, principally Amblyomma americanum (Linnaeus, Acari: Ixodidae), Dermacentor variabilis (Say, Acari: Ixodidae), Ixodes scapularis Say (Acari: Ixodidae), and Rhipicephalus sanguineus (Latreille, Acari: Ixodidae). The majority of funding was provided through US federal agencies, predominantly the Centers for Disease Control and Prevention and the United States Department of Agriculture. Ixodes scapularis was the target of over 50% of identified articles, with the majority of research conducted within 3 states in the Northeast region of the U.S. Only 8.2% of included studies evaluated integrated tick management interventions. We note gaps in tick control research regarding (i) non-Ixodes medically relevant tick species, (ii) endemic range coverage, and (iii) control methodologies evaluated.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"236-276"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142911319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Unusual bot fly larva removed from the scalp of a returning traveler.","authors":"Jerome Goddard, David Cross, Carla Huston","doi":"10.1093/jme/tjae148","DOIUrl":"10.1093/jme/tjae148","url":null,"abstract":"<p><p>An aberrant bot fly specimen was removed from the scalp of a patient 3 wk after returning from Belize. The specimen showed little resemblance to the typical human bot fly larva, Dermatobia hominis, prompting a molecular identification using cytochrome oxidase I and II (COI and COII, respectively) mitochondrial DNA sequence regions. A BLAST search was subsequently performed, and both our COI and COII amplicon sequences showed 99-100% match with Dermatobia hominis, despite the specimen's clearly aberrant morphology. These findings suggest there is much wider variation in Dermatobia larval morphology than previously known or perhaps that there are new or cryptic species within this group.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"458-460"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rachel McNeal, Jeffrey D Wells, Jeffery K Tomberlin
{"title":"Bernard Greenberg: a legacy in medical, veterinary, and forensic entomology.","authors":"Rachel McNeal, Jeffrey D Wells, Jeffery K Tomberlin","doi":"10.1093/jme/tjae158","DOIUrl":"10.1093/jme/tjae158","url":null,"abstract":"<p><p>Bernard Greenberg was a ground-breaking scientist in the worlds of medical-veterinary and forensic entomology, studying the ability of flies to serve as a vector of human and other vertebrate pathogens. His work also extended beyond these topics, creating key studies on flies and their associated microbial ecology. These efforts led to numerous research publications and two books on flies and their associated microorganisms. Greenberg served a pioneering role in establishing the field of forensic entomology in the USA later in his career, publishing key papers and a book that are highly cited to this day. We present a review of Dr Greenberg's scientific contributions concerning flies and disease, insect/microbe interactions, and insects as forensic indicators.</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"231-235"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142960772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sierra M Schluep, Tse-Yu Chen, Shelley A Whitehead, Eva A Buckner
{"title":"Time-of-day changes in permethrin susceptibility and metabolic gene expression in Florida Aedes aegypti (Diptera: Culicidae).","authors":"Sierra M Schluep, Tse-Yu Chen, Shelley A Whitehead, Eva A Buckner","doi":"10.1093/jme/tjaf013","DOIUrl":"10.1093/jme/tjaf013","url":null,"abstract":"<p><p>Aedes aegypti (Linnaeus) is the principal mosquito vector for many of the most medically significant arboviruses that threaten global public health. A better understanding of time-of-day variation in insecticide resistance mediated by detoxifying enzymes in Ae. aegypti could allow for targeted insecticide applications when susceptibility is highest and the upregulation of detoxification enzymes is lowest. Using a susceptible and metabolically resistant field Ae. aegypti strain from Florida, we investigated simulated photoperiodic changes in permethrin susceptibility and upregulation of detoxification enzymes by measuring permethrin LD50 and expression of detoxification genes (GSTE7, GSTE2, CCEae3A, CYP9J28, and CYPBB2) for both strains every 4 h over a 24 h (12:12 h light: dark) cycle. We found that in both Ae. aegypti strains, permethrin susceptibility was lower during the day as compared to evening, with susceptibility lowest at dusk (18:00) and highest between 02:00 and 14:00. Although no significant changes in gene expression over time were observed in the susceptible Ae. aegypti strain, we documented increased expression of all investigated detoxification genes in the metabolically resistant field Ae. aegypti strain during the night (18:00 to 02:00) as compared to the day (06:00 to 14:00). These data suggest that permethrin applications made between midnight and dawn (06:00) may be more effective against Ae. aegypti as compared to applications made at dusk (approximately 18:00).</p>","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"389-398"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}