Journal of medical entomology最新文献

Entomological surveillance of container-breeding mosquitoes focusing on Aedes (Stegomyia) (Diptera: Culicidae) vectors along altitudinal range in Nepal. 对尼泊尔海拔高度范围内以伊蚊（Stegomyia）（双翅目：库蚊科）病媒为重点的容器繁殖蚊虫进行昆虫学监测。

Journal of medical entomology Pub Date : 2024-11-06 DOI: 10.1093/jme/tjae141

Punya Ram Sukupayo, Ram Chandra Poudel, Tirth Raj Ghimire

{"title":"Entomological surveillance of container-breeding mosquitoes focusing on Aedes (Stegomyia) (Diptera: Culicidae) vectors along altitudinal range in Nepal.","authors":"Punya Ram Sukupayo, Ram Chandra Poudel, Tirth Raj Ghimire","doi":"10.1093/jme/tjae141","DOIUrl":"https://doi.org/10.1093/jme/tjae141","url":null,"abstract":"Mosquitoes are a significant public health concern due to their role in transmitting various diseases. This study aimed to investigate mosquitoes' diversity, abundance, and ecological aspects, mainly focusing on Aedes (Diptera: Culicidae) mosquitoes, in central Nepal. The research explored variations across regions, seasons, altitudes, and years. Fieldwork for mosquito collection was conducted between May 2022 and October 2023. Dipping and pipetting methods were employed to collect larvae and pupae, whereas Biogents-Mosquitaire trap captured adult mosquitoes. A total of 7,223 (3,640 larvae and 3,583 adults) mosquitoes, belonging to 8 genera and 18 species, were collected and analyzed. Additionally, a survey examined 5,941 wet containers of 20 different types to assess potential breeding sites. The study revealed Culex pipiens (Linnaeus, 1758) (34.13%) and Aedes albopictus (Skuse, 1895) (27.36%) as the most abundant species. Interestingly, larvae were predominantly Aedes spp. (66.13%), whereas only 13.76% of adults belonged to this genus. Mosquito abundance varied across locations and altitudes, with Siwalik region (331-700 m asl) exhibiting the highest numbers. The monsoon season showed the highest overall abundance (1,492). Used tires were identified as significant breeding sites for Aedes mosquitoes, and infestation rates were higher in shaded containers. Seasonal analysis showed the House Index (HI) reaching its peak (10.92%) and the Breteau Index (BI) reaching 23.08% during the monsoon. Conversely, the Container Index (CI) reached its highest point (37.67%) in the post-monsoon season. The results emphasize the need for comprehensive disease prevention strategies at local and national levels, including public awareness campaigns, to address mosquito-borne illnesses in this famous tourist region.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142592118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of a novel Pavlovskyella (Acari: Argasidae) from Chile, parasite of the culpeo fox (Lycalopex culpaeus). 来自智利的新型 Pavlovskyella（蛔虫：蛔虫科）的鉴定和特征描述，它是 culpeo 狐（Lycalopex culpaeus）的寄生虫。

Journal of medical entomology Pub Date : 2024-11-04 DOI: 10.1093/jme/tjae136

Sebastián Muñoz-Leal, Alexander R Kneubehl, Karen Ardiles, Catalina Parragué-Migone, Ignacio Troncoso-Toro, Pablo Oyarzún-Ruiz, Job E Lopez

{"title":"Identification and characterization of a novel Pavlovskyella (Acari: Argasidae) from Chile, parasite of the culpeo fox (Lycalopex culpaeus).","authors":"Sebastián Muñoz-Leal, Alexander R Kneubehl, Karen Ardiles, Catalina Parragué-Migone, Ignacio Troncoso-Toro, Pablo Oyarzún-Ruiz, Job E Lopez","doi":"10.1093/jme/tjae136","DOIUrl":"https://doi.org/10.1093/jme/tjae136","url":null,"abstract":"Soft ticks (Argasidae) of the subgenus Pavlovskyella Pospelova-Shtrom are worldwide distributed parasites of medical importance. However, the systematics of the subgenus are currently under debate because genetic data shows that the group is paraphyletic. Meanwhile, species of Pavlovskyella continue to be discovered. In this study a novel species of the subgenus is described from specimens collected on a fox in central Chile. The larva of this new species differentiates from other Pavlovskyella spp. by having the following combination of characters: subpyriform dorsal plate; 15 pairs of setae, 7 anterolateral, 3 central, and 5 posterolateral, and hypostome with denticles in the distal third. Nymphs and adults of the species lack cheeks, eyes or bulging structures on the flank, but exhibit dorsoventral grooves, and humps on tarsi I, II, and III. Moreover, a patch of glabrous integument appears on the distal portion of coxal folds. A phylogenetic analysis using the mitogenome indicates a monophyletic group composed by Ornithodoros (Pavlovskyella) brasiliensis Aragão, Ornithodoros (Pavlovskyella) furcosus Neumann, Ornithodoros (Pavlovskyella) improvisus Muñoz-Leal & Venzal, and Ornithodoros (Pavlovskyella) rostratus Aragão. Interestingly, a phylogeny using 18S-28S rDNA sequences shows that South American Pavlovskyella spp. are paraphyletic, as depicted in previous studies. Adding species of the subgenus from the Neotropical region to phylogenetic analyses could aid to solve this paraphyly. Furthermore, this is the fifth species of Pavlovskyella described in South America, and the second in Chile.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ticks and hemoplasma screening in dromedary camels (Camelus dromedarius) from Somalia. 索马里单峰骆驼（Camelus dromedarius）的蜱虫和血浆筛查。

Journal of medical entomology Pub Date : 2024-11-04 DOI: 10.1093/jme/tjae138

Flávia C M Collere, Larissa D R Ferrari, Aamir M Osman, Ahmed A Hassan-Kadle, Mohamed A Shair, Vanessa S Coradi, Abdalla M Ibrahim, Thiago F Martins, Abdulkarim A Yusuf, Ivan R de Barros-Filho, Rogério R Lange, Marcos R André, Thállitha S W J Vieira, Rosangela Z Machado, Rafael F C Vieira

{"title":"Ticks and hemoplasma screening in dromedary camels (Camelus dromedarius) from Somalia.","authors":"Flávia C M Collere, Larissa D R Ferrari, Aamir M Osman, Ahmed A Hassan-Kadle, Mohamed A Shair, Vanessa S Coradi, Abdalla M Ibrahim, Thiago F Martins, Abdulkarim A Yusuf, Ivan R de Barros-Filho, Rogério R Lange, Marcos R André, Thállitha S W J Vieira, Rosangela Z Machado, Rafael F C Vieira","doi":"10.1093/jme/tjae138","DOIUrl":"https://doi.org/10.1093/jme/tjae138","url":null,"abstract":"Hemotropic mycoplasmas (hemoplasmas) are small pleomorphic bacteria that parasitize the surface of red blood cells of mammals. Hemoplasmas have been described in different species from the Camelidae Family, such as llamas and alpacas (South American camelids), but data on dromedary camels (Camelus dromedarius) are limited to a few reports. Somalia has one of the world's largest dromedary camel populations, and studies on hemoplasmas and tick-borne pathogens are lacking. Accordingly, this study aimed to screen dromedaries from Somalia for hemoplasmas by PCR-based assays. A total of 155 dromedary camel blood samples from 2 different areas of Mogadishu (n = 104) and the Lower Shabelle Region (n = 51) of the country were collected. All blood DNA samples were screened for hemoplasmas using a SYBR Green Universal Real-Time PCR (qPCR), nested PCR (nPCR), and conventional PCR (cPCR) assays targeting the 16S rRNA gene of hemoplasmas. Five out of 155 animals (3.23%; 95% confidence interval [CI]: 1.39-7.33%) were positive for hemoplasmas. A total of 346 (228 M, 117 F, and 1 nymph) ticks were collected from 79/155 (50.9%; 95% CI: 42.8-59.1%) dromedary camels with a mean of 4.4 ticks per animal. Ticks were identified as Rhipicephalus pulchellus (174/346; 50.3%), Hyalomma dromedarii (103/346; 29.8%), Hyalomma rufipes (35/346; 10.1%), Hyalomma marginatum (16/346; 4.6%), Rhipicephalus humeralis (14/346; 4.0%), Amblyomma lepidum (2/346; 0.6%), Amblyomma gemma (1/346; 0.3%), and Ornithodoros sp. (1/185; 0.5). This is the first study on the molecular screening for hemoplasmas in dromedary camels from Somalia and the first report of A. lepidum and R. humeralis in Somali dromedary camels.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novo plant-based mosquito repellent shows promise for exclusion of Aedes mosquitoes from "window" entry. 诺沃植物驱蚊剂有望从 "窗户 "入口处驱除伊蚊。

Journal of medical entomology Pub Date : 2024-11-01 DOI: 10.1093/jme/tjae137

Sare I Yavasoglu, Martyn J Wood, James C Bull, Nergis Alkış, Emrecan Doğan, Abeer M Alkhaibari, Tariq M Butt

{"title":"Novo plant-based mosquito repellent shows promise for exclusion of Aedes mosquitoes from \"window\" entry.","authors":"Sare I Yavasoglu, Martyn J Wood, James C Bull, Nergis Alkış, Emrecan Doğan, Abeer M Alkhaibari, Tariq M Butt","doi":"10.1093/jme/tjae137","DOIUrl":"https://doi.org/10.1093/jme/tjae137","url":null,"abstract":"Mosquitoes threaten over half of the world's population through vectored diseases such as malaria, zika, yellow fever, dengue, and chikungunya. Mosquitoes have a highly developed olfactory system attuned to chemotaxis relating to host-seeking, mating, and oviposition behavior. In this study, we aimed to determine the spatial efficacy of 2 plant-based repellent blends (Blend3 and Blend4 that had previously been found to successfully repel Aedes, Anopheles and Culex mosquitoes in wind tunnel assays) in excluding Aedes aegypti from the window entry. A new cage system was developed for parallel \"no-choice\" and \"choice\" olfactometric assays. In the no-choice trial, Blends 3 and 4, as well as commercial products (N, N-diethyl-3-methylbenzamide, p-menthane-3,8-diol [PMD], 3-(N-n-butyl-N-acetyl)-amino-propionic acid ethyl ester, and 2-(2-hydroxyethyl)-1-methylpropylstyrene 1-piperidine carboxylate), were adsorbed into filter papers of different sizes and placed in a window created between 2 attached bug dorms. Then, the number of mosquitoes entering the window was counted through a 6-min period. In choice olfactometric assays, Blends 3, 4, and PMD were adsorbed into filter paper and the number of mosquitoes moving away from Blend 3 and PMD were compared. No-choice assays showed that Blend3 (P < 0.001) and Blend4 (P = 0.0012) were more repellent than the best commercial product PMD. Additionally, while Blend 4 was significantly more repellent than Blend 3 (P = 0.012) in the choice assay, overall, these 2 blends show promise as new repellents for the spatial exclusion of Aedes aegypti from window entry alone or as part of a \"push-pull'' strategy.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dermacentor species (Acari: Ixodidae) in western Canada, with detection of Dermacentor similis. 加拿大西部的 Dermacentor 种类（蛔虫：Ixodidae），并发现了 Dermacentor similis。

Journal of medical entomology Pub Date : 2024-11-01 DOI: 10.1093/jme/tjae133

Grace K Nichol, Paula Lado, Louwrens P Snyman, Shaun J Dergousoff, J Scott Weese, Amy L Greer, Katie M Clow

{"title":"Dermacentor species (Acari: Ixodidae) in western Canada, with detection of Dermacentor similis.","authors":"Grace K Nichol, Paula Lado, Louwrens P Snyman, Shaun J Dergousoff, J Scott Weese, Amy L Greer, Katie M Clow","doi":"10.1093/jme/tjae133","DOIUrl":"https://doi.org/10.1093/jme/tjae133","url":null,"abstract":"Numerous tick species are undergoing significant range expansion in Canada, including several Dermacentor spp Koch (Acari: Ixodidae). With the recent description of Dermacentor similis Lado in the western United States, additional research is required to determine the current range of this species. Five hundred ninety-eight Dermacentor spp. were collected from companion animals in the western Canadian provinces of British Columbia, Alberta, and Saskatchewan. Ticks were morphologically identified to species, followed by PCR and gel electrophoresis of the ITS-2 partial gene target (n = 595). Ninety-seven percent (n = 579/595) generated valid banding patterns. The banding pattern for the majority (74%, n = 206/278) of Dermacentor spp. from southern British Columbia was consistent with D. variabilis (Say), while 26% (n = 72/278) was consistent with D. andersoni Stiles. For samples from Alberta, 38% (n = 3/8) had banding patterns consistent with D. variabilis and 63% (n = 5/8) with D. andersoni. All (n = 293) ticks from Saskatchewan had banding patterns consistent with D. variabilis. After the description of D. similis was published, DNA sequencing of mitochondrial (16S rDNA gene, COI gene) and nuclear (ITS-2) markers was used to confirm the identity of 40 samples. Twenty-seven samples that had banding patterns consistent with D. variabilis from British Columbia were confirmed to be D. similis. One sample from Alberta and five from Saskatchewan were confirmed to be D. variabilis and seven samples from British Columbia were D. andersoni. The ITS-2 amplicons were not useful for differentiating between D. variabilis and D. similis. These results provide evidence of D. similis in western Canada and highlight that sequences of the mitochondrial genes are effective for distinguishing D. andersoni, D. variabilis, and D. similis.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142565397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Published questionnaires and clinical observation surveys on traumatic sheep myiasis: optimizing survey design and the integration of these two survey methods. 关于外伤性羊肌炎的已发布问卷和临床观察调查：优化调查设计并整合这两种调查方法。

Journal of medical entomology Pub Date : 2024-10-28 DOI: 10.1093/jme/tjae132

Tharindu Bandara Bambaradeniya, Paola Annarosa Magni, Ian Robert Dadour

引用次数: 0

Comparative ecological analysis and predictive modeling of tick-borne pathogens. 蜱传病原体的比较生态分析和预测模型。

Journal of medical entomology Pub Date : 2024-10-23 DOI: 10.1093/jme/tjae127

William Manley, Tam Tran, Melissa Prusinski, Dustin Brisson

{"title":"Comparative ecological analysis and predictive modeling of tick-borne pathogens.","authors":"William Manley, Tam Tran, Melissa Prusinski, Dustin Brisson","doi":"10.1093/jme/tjae127","DOIUrl":"https://doi.org/10.1093/jme/tjae127","url":null,"abstract":"Tick-borne diseases constitute the predominant vector-borne health threat in North America. Recent observations have noted a significant expansion in the range of the black-legged tick (Ixodes scapularis Say, Acari: Ixodidae), alongside a rise in the incidence of diseases caused by its transmitted pathogens: Borrelia burgdorferi Johnson (Spirochaetales: Spirochaetaceae), Babesia microti Starcovici (Piroplasmida: Babesiidae), and Anaplasma phagocytophilium Zhu (Rickettsiales: Anaplasmataceae), the causative agents of Lyme disease, babesiosis, and anaplasmosis, respectively. Prior research identified environmental features that influence the ecological dynamics of I. scapularis and B. burgdorferi that can be used to predict the distribution and abundance of these organisms, and thus Lyme disease risk. In contrast, there is a paucity of research into the environmental determinants of B. microti and A. phagocytophilium. Here, we use over a decade of surveillance data to model the impact of environmental features on the infection prevalence of these increasingly common human pathogens in ticks across New York State (NYS). Our findings reveal a consistent northward and westward expansion of B. microti in NYS from 2009 to 2019, while the range of A. phagocytophilum varied at fine spatial scales. We constructed biogeographic models using data from over 650 site-year visits and encompassing more than 250 environmental variables to accurately forecast infection prevalence for each pathogen to a future year that was not included in model training. Several environmental features were identified to have divergent effects on the pathogens, revealing potential ecological differences governing their distribution and abundance. These validated biogeographic models have applicability for disease prevention efforts.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Are they there, how many, and how big? Investigating potential trap biases in the surveillance of La Crosse virus vectors. 它们是否存在，有多少，有多大？调查拉克罗斯病毒载体监测中潜在的陷阱偏差。

Journal of medical entomology Pub Date : 2024-10-23 DOI: 10.1093/jme/tjae126

Corey A Day, Rebecca T Trout Fryxell

{"title":"Are they there, how many, and how big? Investigating potential trap biases in the surveillance of La Crosse virus vectors.","authors":"Corey A Day, Rebecca T Trout Fryxell","doi":"10.1093/jme/tjae126","DOIUrl":"https://doi.org/10.1093/jme/tjae126","url":null,"abstract":"Several methods of mosquito collection are used for the surveillance of the primary La Crosse virus (LACV) vectors, Aedes triseriatus (Say, 1823), Ae. albopictus (Skuse, 1895), and Ae. japonicus (Theobald, 1901). However, little is known about how the choice of collection method may confound inferences made from LACV vector surveillance data. Therefore, the objective of this study was to investigate potential biases in the surveillance of LACV vectors using the Biogents BG-Sentinel 2 (BGS), CDC-Light Trap (CDC-LT), Biogents Gravid Aedes Trap (BG-GAT), and standard oviposition cup (ovicup). The traps were deployed simultaneously at 10 sites in Knovxille, Tennessee, USA for 20 consecutive weeks. Surveillance results differed widely among the traps, demonstrating a strong potential for trap biases in LACV vector surveillance. The BGS and CDC-LT were effective for collecting Ae. albopictus but were not sensitive to the presence of Ae. triseriatus or Ae. japonicus. The ovicup was the best trap for detecting Ae. triseriatus, while the BG-GAT was the only trap that regularly collected Ae. japonicus. Surveillance conducted with the CDC-LT or BGS indicated that Ae. albopictus was dominant at all sites, but the ovicup and BG-GAT suggested a much larger relative abundance of Ae. triseriatus and Ae. japonicus, respectively. Aedes albopictus and Ae. triseriatus collected in the BG-GAT were significantly larger than those collected from the BGS and CDC-LT, indicating that the traps sampled different sub-populations. A multi-method surveillance approach is recommended to reduce potential biases when conducting surveillance of LACV vectors.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dengue-mediated changes in the vectorial capacity of Aedes aegypti (Diptera: Culicidae): manipulation of transmission or infection by-product? 登革热介导的埃及伊蚊（双翅目：Culicidae）媒介能力的变化：传播操纵还是感染副产品？

Journal of medical entomology Pub Date : 2024-10-22 DOI: 10.1093/jme/tjae134

Ioana Mateescu, Sebastian Lequime

{"title":"Dengue-mediated changes in the vectorial capacity of Aedes aegypti (Diptera: Culicidae): manipulation of transmission or infection by-product?","authors":"Ioana Mateescu, Sebastian Lequime","doi":"10.1093/jme/tjae134","DOIUrl":"https://doi.org/10.1093/jme/tjae134","url":null,"abstract":"An arthropod's vectorial capacity summarizes its disease transmission potential. Life-history traits, such as fecundity or survival, and behavioral traits, such as locomotor activity, host-seeking and feeding behavior, are important components of vectorial capacity. Studies have shown that mosquito-borne pathogens may alter important vectorial capacity traits of their mosquito vectors, thus directly impacting their transmission and epidemic potential. Here, we compile and discuss the evidence supporting dengue-mediated changes in the yellow fever mosquito Aedes aegypti (L.), its primary vector, and evaluate whether the observed effects represent an evolved trait manipulation with epidemiological implications. Dengue infection appears to manipulate essential traits that facilitate vector-host contact, such as locomotor activity, host-seeking, and feeding behavior, but the underlying mechanisms are still not understood. Conversely, life-history traits relevant to vector population dynamics, such as survival, oviposition, and fecundity, appear to be negatively impacted by dengue virus. Overall, any detrimental effects on life-history traits may be a negligible cost derived from the virulence that dengue has evolved to facilitate its transmission by manipulating Ae. aegypti behavior and feeding performance. However, methodological disparities among studies render comparisons difficult and limit the ability to reach well-supported conclusions. This highlights the need for more standardized methods for the research into changes in virus-mediated traits. Eventually, we argue that the effects on life-history traits and behavior outlined here must be considered when assessing the epidemiological impact of dengue or other arbovirus-vector-host interactions.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic characterization of immune adaptor molecule MyD88 in Culex pipiens complex (Diptera: Culicidae) mosquitoes from China. 中国喙库蚊（双翅目：库蚊科）免疫适应分子MyD88的遗传特征。

Journal of medical entomology Pub Date : 2024-10-22 DOI: 10.1093/jme/tjae128

Xueting Wang, Lilan Zhu, Caifei Huo, Dan He, Haifeng Tian, Xiaolan Fan, Yongqing Lyu, Yan Li

{"title":"Genetic characterization of immune adaptor molecule MyD88 in Culex pipiens complex (Diptera: Culicidae) mosquitoes from China.","authors":"Xueting Wang, Lilan Zhu, Caifei Huo, Dan He, Haifeng Tian, Xiaolan Fan, Yongqing Lyu, Yan Li","doi":"10.1093/jme/tjae128","DOIUrl":"https://doi.org/10.1093/jme/tjae128","url":null,"abstract":"Mosquitoes of the Culex (Cx.) pipiens complex are vectors of severe diseases including West Nile fever by West Nile virus, Japanese encephalitis by Japanese encephalitis virus, and Lymphatic filariasis by filarial nematode Wuchereria bancrofti. As a major portion of mosquito immune system, the Toll pathway implicates in response against infections of mosquito-borne pathogens and biocontrol agents. The genetic diversity of immune-related molecules is expected to be a feasible and effective introduction to expand our knowledge of the mosquito-microbe interplay. However, a comprehensive description is currently lacking regarding the genetic characteristic of the Toll pathway molecules in Cx. pipiens complex mosquitoes. In the present study, genetic changes in Cx. pipiens complex MyD88 (Myeloid differentiation primary response protein 88) were analyzed as a precedent for the Toll pathway molecules in this taxon. MyD88 is a critical adaptor of the pathway transducing signals from TIR-containing receptors to downstream death domain-containing molecules. Our results revealed that adaptive selection has influenced the genetic changes of the molecule, giving rise to acceleration of diversity at a number of amino acid sites. The adaptively selected sites lie in the death domain, intermediate domain, and C-terminal extension. The characteristics of the genetic changes shed insights into the prominent molecular-level structural basis and the involvement strategy of the adaptor in the arms race against exogenous challenges. This finding would be beneficial for further exploration and deeper understanding of the mosquitoes' vectorial capacity and facilitating the effectiveness and sustainability of the biocontrol agents.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0