Journal of medical entomology最新文献_第2页

Blood-feeding stable flies, Stomoxys calcitrans (Diptera: Muscidae), are attracted to, and transmit Staphylococcus aureus (Bacillales: Staphylococcaceae), a causal agent of bovine mastitis: a laboratory pilot study. 食血厩蝇（Stomoxys calcitrans，双翅目：鹟科）会被金黄色葡萄球菌（芽孢杆菌科：葡萄球菌属）吸引并传播金黄色葡萄球菌，金黄色葡萄球菌是牛乳腺炎的病原体：一项实验室试验研究。

Journal of medical entomology Pub Date : 2024-11-14 DOI: 10.1093/jme/tjae101

Saif Nayani, Sanam Meraj, Asim Renyard, Gerhard Gries

{"title":"Blood-feeding stable flies, Stomoxys calcitrans (Diptera: Muscidae), are attracted to, and transmit Staphylococcus aureus (Bacillales: Staphylococcaceae), a causal agent of bovine mastitis: a laboratory pilot study.","authors":"Saif Nayani, Sanam Meraj, Asim Renyard, Gerhard Gries","doi":"10.1093/jme/tjae101","DOIUrl":"10.1093/jme/tjae101","url":null,"abstract":"Stable flies, Stomoxys calcitrans (Linnaeus) (Diptera: Muscidae), are common blood-feeding ectoparasites of cows and thus potential vectors of the skin-dwelling bacterium Staphylococcus aureus, a causal agent of bovine mastitis which inflicts udder inflammation in cows. Our objectives were to determine whether stable flies (i) are attracted to disease-causing strains of S. aureus, and (ii) transmit S. aureus from infected blood to sterile blood. In 3-chamber olfactometers, five of eight S. aureus strains grown on agar and tested versus sterile agar attracted female stable flies. When flies ingested droplets of blood inoculated with S. aureus at doses of 0 (control), 105 (low), 107 (medium), and > 109 (high) colony-forming units per milliliter and subsequently ingested sterile blood, they transmitted S. aureus to the sterile blood. The dose of S. aureus in blood droplets fed upon by flies during their first feeding bout dose-dependently affected the amount of bacteria that flies transmitted to sterile blood during their second feeding bout, but the time elapsed between feeding bouts (0 h, 1 h, 8 h, and 24 h) had no effect on the amount of microbes transmitted to sterile blood. Our data infer the existence of a positive feedback loop. First, stable flies carrying S. aureus and feeding on cows transmit S. aureus, thereby causing mastitis. As S. aureus bacteria of afflicted cows proliferate, they attract even more flies which, in turn, worsen the infection. This type of feedback loop underscores the need for effective stable fly control tactics that curtail the incidence of bovine mastitis in cows.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1470-1477"},"PeriodicalIF":0.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11562966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142116588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of Trichophoromyia jariensis, a new species of phlebotomine sand fly (Diptera: Psychodidae) from the eastern Amazon. 描述来自亚马逊东部的沙蝇新种 Trichophoromyia jariensis（双翅目：食蚜蝇科）。

Journal of medical entomology Pub Date : 2024-11-14 DOI: 10.1093/jme/tjae095

Keison S Cavalcante, Bruno L Rodrigues, Laura Posada-López, Taires Peniche, José F Saraiva, Allan Kardec R Galardo, Eunice A B Galati

引用次数: 0

Commercial products are not effective at repelling European deer keds, Lipoptena cervi (Diptera: Hippoboscidae) but may increase mortality after exposure. 商业产品不能有效驱赶欧洲鹿荨麻（Lipoptena cervi）（双翅目：Hippoboscidae），但可能会增加接触后的死亡率。

Journal of medical entomology Pub Date : 2024-11-14 DOI: 10.1093/jme/tjae109

Michael J Skvarla, Karen Poh, Calvin Norman, Erika T Machtinger

{"title":"Commercial products are not effective at repelling European deer keds, Lipoptena cervi (Diptera: Hippoboscidae) but may increase mortality after exposure.","authors":"Michael J Skvarla, Karen Poh, Calvin Norman, Erika T Machtinger","doi":"10.1093/jme/tjae109","DOIUrl":"10.1093/jme/tjae109","url":null,"abstract":"European deer keds, Lipoptena cervi (Linnaeus, 1758), are hematophagous ectoparasitic flies known to bite cervids and noncervids, including humans. To prevent deer keds from landing and biting hosts, 5 commercially available insect repellents (DEET, IR3535, oil of lemon eucalyptus (OLE), picaridin, and permethrin) and water control were evaluated to determine repellency efficacy and postexposure mortality of deer keds. While there was a significant difference between the groups tested, a post hoc analysis revealed that no treatment was significantly different from the water control. Deer ked survival was different amongst the treatments, with deer keds exposed to permethrin dying much sooner than those exposed to other treatments or control (median survival for permethrin = 0.58 h). Post-hoc pairwise comparisons revealed that deer keds exposed to DEET or IR3535 had similar survival rates (4.82 and 5.15 h, respectively). Still, there were significantly lower survival times for DEET compared to OLE (6.33 h) and picaridin (15.00 h). Deer keds exposed to the water control survived the longest (23.12 h). Overall, deer ked repellency was not significantly different from the control, but permethrin-treated clothes can effectively kill deer keds in a short amount of time, thereby protecting those who recreate outdoors or encounter animals carrying deer keds.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1435-1442"},"PeriodicalIF":0.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laboratory maintenance and care of Rhodnius prolixus (Hemiptera: Reduviidae) and other Triatominae. Rhodnius prolixus（半翅目：Reduviidae）和其他 Triatominae 的实验室维护和保养。

Journal of medical entomology Pub Date : 2024-11-14 DOI: 10.1093/jme/tjae119

Alice C Sutcliffe, Ellen M Dotson

{"title":"Laboratory maintenance and care of Rhodnius prolixus (Hemiptera: Reduviidae) and other Triatominae.","authors":"Alice C Sutcliffe, Ellen M Dotson","doi":"10.1093/jme/tjae119","DOIUrl":"10.1093/jme/tjae119","url":null,"abstract":"Triatomines (Hemiptera: Reduviidae) are hematophagous insects that transmit Trypanosoma cruzi, the etiological agent for Chagas disease, to humans and other mammals. As medically important vectors, species such as Rhodnius prolixus (Hemiptera: Reduviidae) have long been used as a model organism for physiological studies. Laboratory rearing of triatomines is needed to support vector and parasite research. Many environmental conditions, such as suitable housing containers, light source and duration, temperature, humidity, and density, must be addressed when adapting triatomines from a natural habitat for artificial rearing to create conditions for optimal growth and survival. Food source is also an important factor, as triatomines are considered the obligate blood feeders. Parasites and pathogens present risks not only for triatomines but also for the laboratorians handling them. Equipping an insectary space should apply best practices to ensure community, personnel, and insect health. Various triatomine colonies have been maintained in the Centers for Disease Control and Prevention (CDC) Entomology Branch insectary for over 25 years and have more recently been made available to the research community through the Biodefense and Emerging Infections Research Resources Repository (BEI Resources). The CDC Rhodnius prolixus genome has been characterized and thus represents an opportunity for continued model organism research. In addition to fulfilling requests for live triatomines, inquiries are received for support in establishing new and troubleshooting existing laboratory colonies. To accompany the extensive MR4 manual, Methods in Anopheles Research, procedures for triatomine husbandry have been developed and are shared here to address the aforementioned topics.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1345-1367"},"PeriodicalIF":0.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142304984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly abundant bacteria in the gut of Triatoma dimidiata (Hemiptera: Reduviidae) can inhibit the growth of Trypanosoma cruzi (Kinetoplastea: Trypanosomatidae). Triatoma dimidiata（半翅目：Reduviidae）肠道中丰富的细菌可抑制克鲁斯锥虫（Kinetoplastea：Trypanosomatidae）的生长。

Journal of medical entomology Pub Date : 2024-11-14 DOI: 10.1093/jme/tjae012

Juan Carlos Cambronero-Heinrichs, Diego Rojas-Gätjens, Mónica Baizán, Johan Alvarado-Ocampo, Keilor Rojas-Jimenez, Randall Loaiza, Max Chavarría, Ólger Calderón-Arguedas, Adriana Troyo

{"title":"Highly abundant bacteria in the gut of Triatoma dimidiata (Hemiptera: Reduviidae) can inhibit the growth of Trypanosoma cruzi (Kinetoplastea: Trypanosomatidae).","authors":"Juan Carlos Cambronero-Heinrichs, Diego Rojas-Gätjens, Mónica Baizán, Johan Alvarado-Ocampo, Keilor Rojas-Jimenez, Randall Loaiza, Max Chavarría, Ólger Calderón-Arguedas, Adriana Troyo","doi":"10.1093/jme/tjae012","DOIUrl":"10.1093/jme/tjae012","url":null,"abstract":"Chagas disease, caused by the protozoan Trypanosoma cruzi, is a zoonosis primarily found in rural areas of Latin America. It is considered a neglected tropical disease, and Triatoma dimidiata is the main vector of the parasite in Central America. Despite efforts, Chagas disease continues to be a public health concern, and vector control remains a primary tool to reduce transmission. In this study, we tested the hypothesis that highly abundant bacteria in the gut of T. dimidiata inhibit the growth of T. cruzi. To achieve this, bacterial diversity in the gut of T. dimidiata specimens from Costa Rica was characterized by metabarcoding of the 16S rRNA, microbial isolation was performed, and the effect of freeze-dried supernatants of the isolates on T. cruzi was investigated. Metabarcoding showed that the most abundant genera in the gut were Corynebacterium, Tsukamurella, Brevibacterium, and Staphylococcus. Barcoding and sequences comparison confirmed that 8 of the 30 most abundant amplicon sequence variants (ASVs) were isolated, and 2 of them showed an inhibitory effect on the growth of T. cruzi epimastigotes. These bacteria correspond to isolates of Tsukamurella and Brevibacterium, which were respectively the second and sixth most abundant ASVs in the gut of T. dimidiata. Notably, only the isolate of Brevibacterium showed a significant difference in growth inhibition against epimastigotes of both T. cruzi strains tested. These findings suggest that the gut microbiota of T. dimidiata may play an active role in modulating parasite development.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1333-1344"},"PeriodicalIF":0.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity of the Chagas vector Triatoma dimidiata s.l. (Hemiptera: Reduviidae) across geographic scales in a top-priority area for control. 南美锥虫病病媒 Triatoma dimidiata s.l.（半翅目：Reduviidae）在最优先防治地区不同地理尺度上的遗传多样性。

Journal of medical entomology Pub Date : 2024-11-14 DOI: 10.1093/jme/tjae066

Sergio Melgar, Salvador Castellanos, Lori Stevens, María Carlota Monroy, Patricia L Dorn

{"title":"Genetic diversity of the Chagas vector Triatoma dimidiata s.l. (Hemiptera: Reduviidae) across geographic scales in a top-priority area for control.","authors":"Sergio Melgar, Salvador Castellanos, Lori Stevens, María Carlota Monroy, Patricia L Dorn","doi":"10.1093/jme/tjae066","DOIUrl":"10.1093/jme/tjae066","url":null,"abstract":"Population genetic structure of arthropod disease vectors provides important information on vector movement and climate or other environmental variables that influence their distribution. This information is critical for data-driven vector control. In the first comprehensive study of the genetic structure of T. dimidiata s.l. (Latreille, 1811) we focus on an area of active transmission designated as a top priority for control. We examined a high number of specimens across a broad geographic area along the border of Guatemala and El Salvador including multiple spatial scales using a high number of genome-wide markers. Measuring admixture, pairwise genetic differentiation, and relatedness, we estimated the specimens represented three genetic clusters. We found evidence of movement (migration/gene flow) across all spatial scales with more admixture among locations in El Salvador than in Guatemala. Although there was significant isolation by distance, the 2 close villages in Guatemala showed either the most or least genetic variation indicating an additional role of environmental variables. Further, we found that social factors may be influencing the genetic structure. We demonstrated the power of genomic studies with a large number of specimens across a broad geographic area. The results suggest that for effective vector control movement must be considered on multiple spatial scales along with its contributing factors.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1309-1321"},"PeriodicalIF":0.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141545677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and serological detection of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in domestic dogs in Manaus city, Amazonas, Brazil. 巴西亚马孙马瑙斯市家犬中婴儿利什曼原虫（锥虫：锥虫科）的分子和血清学检测。

Journal of medical entomology Pub Date : 2024-11-14 DOI: 10.1093/jme/tjae110

Hevila Gabrieli Nascimento de Campos, Solange Maria Gennari, Ryan Emiliano da Silva, Herbert Sousa Soares, Jaciara de Oliveira Jorge Costa, Sergio Santos de Azevedo, Arlei Marcili

{"title":"Molecular and serological detection of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in domestic dogs in Manaus city, Amazonas, Brazil.","authors":"Hevila Gabrieli Nascimento de Campos, Solange Maria Gennari, Ryan Emiliano da Silva, Herbert Sousa Soares, Jaciara de Oliveira Jorge Costa, Sergio Santos de Azevedo, Arlei Marcili","doi":"10.1093/jme/tjae110","DOIUrl":"10.1093/jme/tjae110","url":null,"abstract":"Leishmaniasis are zoonosis widely spread in Brazil, caused by the protozoan of the genus Leishmania, which includes several species. The disease manifests itself in the visceral or tegumentary form, and the main reservoir is the dogs. Manaus is the largest city in the Brazilian Amazon region, and despite the importance of the municipality, practically nothing is known about leishmaniosis in humans and animals. The objective of this study was to evaluate the occurrence and risk factors associated with the presence of Leishmania infantum in domiciliated dogs from Manaus. Molecular (polymerase chain reaction) and serological (immunofluorescent antibody test) methods were used as an indication of the circulation of the parasite. Blood samples for 154 domiciled dogs were obtained, and prevalence ratio and analysis of the variables were performed. Serum antibodies anti-Leishmania spp. were detected in 20.8% (95% CI: 14.4%-27.2%). Access to the street and zone of residence (P < 0.01) were associated with higher seropositivity. Molecular diagnosis for L. infantum detected positivity in 60 (39%) of the 154 (95% CI: 31.3%-46.7%) animals, and the variables street access, contact with dogs, and zone of residence were associated with higher frequencies of positivity (P < 0.05). Both serology and molecular diagnosis detected positive dogs in the municipality. This is the first description of the circulation of L. infantum infecting dogs in Manaus. As the municipality is classified as nonendemic, studies of isolation and characterization of the isolate must be done urgently.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1519-1523"},"PeriodicalIF":0.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of different spectral ranges of UV-LED lighting for outdoor mosquito trapping in forested area in Thailand. 比较不同光谱范围的紫外线-LED 照明在泰国森林地区户外诱捕蚊子的效果。

Journal of medical entomology Pub Date : 2024-11-14 DOI: 10.1093/jme/tjae112

Suthat Lhaosudto, Ratchadawan Ngoen-Klan, Vithee Meunworn, Monthathip Kongmee, Jeffrey Hii, Theeraphap Chareonviriyaphap

{"title":"Comparison of different spectral ranges of UV-LED lighting for outdoor mosquito trapping in forested area in Thailand.","authors":"Suthat Lhaosudto, Ratchadawan Ngoen-Klan, Vithee Meunworn, Monthathip Kongmee, Jeffrey Hii, Theeraphap Chareonviriyaphap","doi":"10.1093/jme/tjae112","DOIUrl":"10.1093/jme/tjae112","url":null,"abstract":"Mosquito surveillance is critical for actively tracking the location and monitoring population levels and the threat of mosquito-borne disease. Although light-emitting diodes (LEDs) light traps have grown in popularity, there is still a limited understanding of the application of light wavelengths for trapping nocturnally active wild mosquitoes in forest ecotypes. This study evaluated the performance of different UV wavelengths in trapping mosquito populations in a forested mountainous area in Nakhon Ratchasima province, Thailand. Traps with different UV wavelengths were deployed in 6 locations, following a 6 × 6 Latin square replicated 6 times over a total of 36 nights. Light traps were operated between 18:00 and 06:00 h from October 2022 to August 2023. Mosquitoes were separately collected from individual traps every 4 h at 22.00, 2.00, and 6.00 h. Mosquitoes were killed by placing in a freezer (- 20 °C) for at least 30 min and then were morphologically identified using illustrated keys for adult females. Traps fitted with the LED 365 wavelength light source were the most effective in capturing 790 (23.66%) of the total mosquitoes collected, followed by the UV fluorescent 632 (18.93%), with the other 4 LED wavelengths collecting between 16.89% (LED 385) and 12.64% (LED 375) of the mosquitoes. Culex was the most common genus, representing 56.00% of total mosquito abundance. LED 365 and LED 385 were comparable to the UV fluorescent traps (the standard reference). Optimal trapping times were during 18:00-22:00 h. Compared to the other wavelengths, LED 365 was significantly more effective at capturing Coquillettidia and Culex mosquitoes than the UV-based traps.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1510-1518"},"PeriodicalIF":0.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142116589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phlebotomine sand fly (Diptera: Psychodidae) fauna, blood meal source, and detection of Leishmania (Kinetoplastida: Trypanosomatidae) DNA in the Gurupi Biological Reserve, Eastern Amazon, Brazil. 巴西亚马逊东部古鲁皮生物保护区中的沙蝇（双翅目：食心蝇科）动物群、血餐来源以及利什曼病（Kinetoplastida: Trypanosomatidae）DNA的检测。

Journal of medical entomology Pub Date : 2024-11-14 DOI: 10.1093/jme/tjae108

Judson Chaves Rodrigues, Rosa Cristina Ribeiro-da-Silva, Antonia Suely Guimarães-E-Silva, Maxcilene da Silva de Oliveira, Bruno Leite Rodrigues, Ronayce Conceição de Jesus Serrão Pimenta, Eunice Aparecida Bianchi Galati, Valéria Cristina Soares Pinheiro

{"title":"Phlebotomine sand fly (Diptera: Psychodidae) fauna, blood meal source, and detection of Leishmania (Kinetoplastida: Trypanosomatidae) DNA in the Gurupi Biological Reserve, Eastern Amazon, Brazil.","authors":"Judson Chaves Rodrigues, Rosa Cristina Ribeiro-da-Silva, Antonia Suely Guimarães-E-Silva, Maxcilene da Silva de Oliveira, Bruno Leite Rodrigues, Ronayce Conceição de Jesus Serrão Pimenta, Eunice Aparecida Bianchi Galati, Valéria Cristina Soares Pinheiro","doi":"10.1093/jme/tjae108","DOIUrl":"10.1093/jme/tjae108","url":null,"abstract":"This study was conducted in the Gurupi Biological Reserve (REBIO-Gurupi), the largest area of Amazon rainforest in Maranhão State, Brazil. The objectives were to survey the sand fly (Diptera: Psychodidae) fauna of REBIO-Gurupi, identify blood meal sources, and investigate the presence of Leishmania (Ross, 1903) (Kinetoplastida: Trypanosomatidae) DNA. Individuals were collected using Centers for Disease Control (CDC) light traps and black and white Shannon traps in May and Jun 2022 and Jan 2023. DNA was extracted from female sand flies and subjected to amplification and sequencing of cytochrome b molecular marker (CYTB) for identification of blood meal sources and the first internal transcribed spacer (ITS-1) of ribosomal DNA for Leishmania detection. A total of 514 sand flies individuals were sampled, of which 93 were identified at the genus or series level (9 taxa) and 421 were identified at the species level (24 taxa). Psychodopygus davisi (Root, 1934) (41.1%), Nyssomyia antunesi (Coutinho, 1939) (10.3%), and Psychodopygus (Mangabeira, 1941) Chagasi Series Barretto, 1962 (9.7%) were the most frequently collected. Human (Homo sapiens, Primates, Hominidae) and tapir (Tapirus terrestris, Perissodactyla, Tapiridae) DNA was detected in 10 female sand flies. Leishmania (Leishmania) infantum Cunha and Chagas, 1937 DNA was detected in 2 specimens of Ps. davisi. Given the presence of vectors of Leishmania in REBIO-Gurupi, it is imperative to conduct more comprehensive studies on the interactions among sand flies, Leishmania, and pathogen reservoirs in the area.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1501-1509"},"PeriodicalIF":0.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142121366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecological interactions of Triatoma sanguisuga (Hemiptera: Reduviidae) and risk for human infection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) in Illinois and Louisiana. 伊利诺伊州和路易斯安那州桑吉氏三体虫（半翅目：Reduviidae）与人类感染克鲁兹锥虫（Kinetoplastida：Trypanosomatidae）风险之间的生态相互作用。

Journal of medical entomology Pub Date : 2024-11-14 DOI: 10.1093/jme/tjae017

Eric Dumonteil, Weihong Tu, F Agustín Jiménez, Claudia Herrera

{"title":"Ecological interactions of Triatoma sanguisuga (Hemiptera: Reduviidae) and risk for human infection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) in Illinois and Louisiana.","authors":"Eric Dumonteil, Weihong Tu, F Agustín Jiménez, Claudia Herrera","doi":"10.1093/jme/tjae017","DOIUrl":"10.1093/jme/tjae017","url":null,"abstract":"Triatoma sanguisuga (Leconte) is one of the most widely distributed kissing bugs in the United States, associated with an extensive zoonotic circulation of Trypanosoma cruzi, the agent of Chagas disease, in a large part of the country. However, the actual risk for human infection in the United States is poorly understood. Here, we further assessed the ecology of T. sanguisuga bugs collected in residents' houses in Illinois and Louisiana, using a metagenomic approach to identify their blood-feeding sources, T. cruzi parasites and gut microbiota. Blood meal analysis revealed feeding on domestic animals (dogs, cats, pigs, goats, and turkeys), synanthropic species (raccoons, opossums, and squirrels), as well as the more sylvatic white-tail deer. Human blood was identified in 11/14 (78%) of bugs, highlighting a frequent vector-human contact. The infection rate with T. cruzi was 53% (8/15), and most infected bugs (6/8) had fed on humans. A total of 41 bacterial families were identified, with significant differences in microbiota alpha and beta diversity between bugs from Louisiana and Illinois. However, predicted metabolic functions remained highly conserved, suggesting important constraints to fulfill their role in bug biology. These results confirmed a significant risk for vector-borne transmission of T. cruzi to humans in Louisiana and Illinois, which warrants more active screening for human infections. Also, while there is broad plasticity in the bacterial composition of T. sanguisuga microbiota, there are strong constraints to preserve metabolic profile and function, making it a good target for novel vector control strategies.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"1282-1289"},"PeriodicalIF":0.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139907237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0