Journal of medical entomology最新文献_第3页

Surveillance of tick-borne pathogens present in ticks (Acari: Ixodidae) removed from companion animals in Louisiana, USA. 对美国路易斯安那州从伴侣动物身上清除的蜱虫（Acari: Ixodidae）中存在的蜱传病原体进行监测。

Journal of medical entomology Pub Date : 2025-01-13 DOI: 10.1093/jme/tjae122

Sabrina M Valdes, Sean Simonson, Christine Scott-Waldron, Gary Balsamo, Britton J Grasperge, Lane D Foil, Udeni Balasuriya, Kevin R Macaluso

{"title":"Surveillance of tick-borne pathogens present in ticks (Acari: Ixodidae) removed from companion animals in Louisiana, USA.","authors":"Sabrina M Valdes, Sean Simonson, Christine Scott-Waldron, Gary Balsamo, Britton J Grasperge, Lane D Foil, Udeni Balasuriya, Kevin R Macaluso","doi":"10.1093/jme/tjae122","DOIUrl":"10.1093/jme/tjae122","url":null,"abstract":"Current knowledge of tick distribution and tick-borne pathogen presence across Louisiana is limited. Collaborating with veterinarians across the state, ticks removed from companion animals were recovered and assessed for the presence of zoonotic pathogens. A large number of ticks (n = 959) were removed from companion animals and subsequently screened using qPCR for Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, Bartonella henselae, Ehrlichia chaffeensis, and spotted fever group Rickettsia. Five different tick species, Ixodes scapularis (54.5%), Amblyomma americanum (18.4%), Amblyomma maculatum (12.5%), Dermacentor variabilis (11.2%), and Rhipicephalus sanguineus (0.3%) from different regions of Louisiana were collected from October 2018 to July 2019. There were 15 PCR-positive ticks for Rickettsia parkeri (1.6% prevalence), and four ticks were positive for Ehrlichia chaffeensis (0.4% prevalence). This survey identifies ticks and tick-borne pathogens associated with companion animals and areas for future active surveillance.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"181-188"},"PeriodicalIF":0.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Entomological surveillance of container-breeding mosquitoes focusing on Aedes (Stegomyia) (Diptera: Culicidae) vectors along altitudinal range in Nepal. 对尼泊尔海拔高度范围内以伊蚊（Stegomyia）（双翅目：库蚊科）病媒为重点的容器繁殖蚊虫进行昆虫学监测。

Journal of medical entomology Pub Date : 2025-01-13 DOI: 10.1093/jme/tjae141

Punya Ram Sukupayo, Ram Chandra Poudel, Tirth Raj Ghimire

{"title":"Entomological surveillance of container-breeding mosquitoes focusing on Aedes (Stegomyia) (Diptera: Culicidae) vectors along altitudinal range in Nepal.","authors":"Punya Ram Sukupayo, Ram Chandra Poudel, Tirth Raj Ghimire","doi":"10.1093/jme/tjae141","DOIUrl":"10.1093/jme/tjae141","url":null,"abstract":"Mosquitoes are a significant public health concern due to their role in transmitting various diseases. This study aimed to investigate mosquitoes' diversity, abundance, and ecological aspects, mainly focusing on Aedes (Diptera: Culicidae) mosquitoes, in central Nepal. The research explored variations across regions, seasons, altitudes, and years. Fieldwork for mosquito collection was conducted between May 2022 and October 2023. Dipping and pipetting methods were employed to collect larvae and pupae, whereas Biogents-Mosquitaire trap captured adult mosquitoes. A total of 7,223 (3,640 larvae and 3,583 adults) mosquitoes, belonging to 8 genera and 18 species, were collected and analyzed. Additionally, a survey examined 5,941 wet containers of 20 different types to assess potential breeding sites. The study revealed Culex pipiens (Linnaeus, 1758) (34.13%) and Aedes albopictus (Skuse, 1895) (27.36%) as the most abundant species. Interestingly, larvae were predominantly Aedes spp. (66.13%), whereas only 13.76% of adults belonged to this genus. Mosquito abundance varied across locations and altitudes, with Siwalik region (331-700 m asl) exhibiting the highest numbers. The monsoon season showed the highest overall abundance (1,492). Used tires were identified as significant breeding sites for Aedes mosquitoes, and infestation rates were higher in shaded containers. Seasonal analysis showed the House Index (HI) reaching its peak (10.92%) and the Breteau Index (BI) reaching 23.08% during the monsoon. Conversely, the Container Index (CI) reached its highest point (37.67%) in the post-monsoon season. The results emphasize the need for comprehensive disease prevention strategies at local and national levels, including public awareness campaigns, to address mosquito-borne illnesses in this famous tourist region.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"207-219"},"PeriodicalIF":0.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142592118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Updated checklist with new records and molecular data for the mosquitoes (Diptera: Culicidae) of Hong Kong. 香港蚊子（双翅目：Culicidae）新记录和分子数据更新核对表。

Journal of medical entomology Pub Date : 2025-01-13 DOI: 10.1093/jme/tjae125

Shaolin Han, Elliott F Miot, Yunshi Liao, Pradya Somboon, Ralph E Harbach, Ka Mei Sze-To, Lilia Tsz-Wing Tang, Benoit Guénard, Tommy Tsan-Yuk Lam

{"title":"Updated checklist with new records and molecular data for the mosquitoes (Diptera: Culicidae) of Hong Kong.","authors":"Shaolin Han, Elliott F Miot, Yunshi Liao, Pradya Somboon, Ralph E Harbach, Ka Mei Sze-To, Lilia Tsz-Wing Tang, Benoit Guénard, Tommy Tsan-Yuk Lam","doi":"10.1093/jme/tjae125","DOIUrl":"10.1093/jme/tjae125","url":null,"abstract":"An extensive mosquito survey was carried out in Hong Kong from September to October 2022, employing a variety of collection methods. Specimens were identified using a combination of morphology and mitochondrial cytochrome C oxidase subunit 1 (COI) barcode sequences. Twenty-nine species, including three new records, i.e., Culex bicornutus (Theobald), Culex cinctellus Edwards, and Lutzia chiangmaiensis Somboon & Harbach, were collected. Phylogenetic analysis of COI sequences of Culex annulus Theobald and Culex vishnui Theobald collected in Hong Kong and elsewhere revealed that the sequences of the two nominal species are genetically very similar and are included in the same clade. Consequently, the synonymy of Cx. annulus with Cx. vishnui is reinstated. Lutzia halifaxii (Theobald) is removed from the list of species in Hong Kong and is replaced with Lutzia vorax Edwards, the identification of which is confirmed in the present study. The record of Culex spiculothorax Bram recorded in Hong Kong is replaced with the senior synonym Culex sasai Kano, Nitahara & Awaya. The occurrence of Anopheles fluviatilis James and Aedes aegypti (Linnaeus) is discussed. Finally, an updated checklist of the mosquitoes of Hong Kong, which now includes 76 species representing 14 genera, is provided, with notation of those species that vector pathogens of human diseases.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"99-111"},"PeriodicalIF":0.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A numbers game: mosquito-based arbovirus surveillance in two distinct geographic regions of Latin America. 数字游戏：拉丁美洲两个不同地理区域以蚊子为基础的虫媒病毒监测。

Journal of medical entomology Pub Date : 2025-01-13 DOI: 10.1093/jme/tjae121

Jacqueline Mojica, Valentina Arévalo, Jose G Juarez, Ximena Galarza, Karla Gonzalez, Andrés Carrazco, Harold Suazo, Eva Harris, Josefina Coloma, Patricio Ponce, Angel Balmaseda, Varsovia Cevallos

{"title":"A numbers game: mosquito-based arbovirus surveillance in two distinct geographic regions of Latin America.","authors":"Jacqueline Mojica, Valentina Arévalo, Jose G Juarez, Ximena Galarza, Karla Gonzalez, Andrés Carrazco, Harold Suazo, Eva Harris, Josefina Coloma, Patricio Ponce, Angel Balmaseda, Varsovia Cevallos","doi":"10.1093/jme/tjae121","DOIUrl":"10.1093/jme/tjae121","url":null,"abstract":"Aedes mosquitoes, as vectors of medically important arthropod-borne viruses (arboviruses), constitute a major public health threat that requires entomological and epidemiological surveillance to guide vector control programs to prevent and reduce disease transmission. In this study, we present the collaborative effort of 1 year of Aedes ageypti (Linnaeus, 1762) mosquito-based arbovirus surveillance in 2 geographically distinct regions of Latin America (Nicaragua and Ecuador). Adult female mosquitoes were collected using backpack aspirators in over 2,800 randomly selected households (Nicaragua, Ecuador) and 100 key sites (Nicaragua) from 8 distinct communities (Nicaragua: 2, Ecuador: 6). A total of 1,358 mosquito female pools were processed for RNA extraction and viral RNA detection using real-time reverse transcription-polymerase chain reaction. Ten positive dengue virus (DENV) pools were detected (3 in Nicaragua and 7 in Ecuador), all of which were found during the rainy season and matched the serotypes found in humans (Nicaragua: DENV-1 and DENV-4; Ecuador: DENV-2). Infection rates ranged from 1.13 to 23.13, with the Nicaraguan communities having the lowest infection rates. Our results demonstrate the feasibility of detecting DENV-infected Aedes mosquitoes in low-resource settings and underscore the need for targeted mosquito arbovirus sampling and testing, providing valuable insights for future surveillance programs in the Latin American region.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"220-224"},"PeriodicalIF":0.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735261/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142304983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novo plant-based mosquito repellent shows promise for exclusion of Aedes mosquitoes from "window" entry. 诺沃植物驱蚊剂有望从 "窗户 "入口处驱除伊蚊。

Journal of medical entomology Pub Date : 2025-01-13 DOI: 10.1093/jme/tjae137

Sare I Yavasoglu, Martyn J Wood, James C Bull, Nergis Alkış, Emrecan Doğan, Abeer M Alkhaibari, Tariq M Butt

{"title":"Novo plant-based mosquito repellent shows promise for exclusion of Aedes mosquitoes from \"window\" entry.","authors":"Sare I Yavasoglu, Martyn J Wood, James C Bull, Nergis Alkış, Emrecan Doğan, Abeer M Alkhaibari, Tariq M Butt","doi":"10.1093/jme/tjae137","DOIUrl":"10.1093/jme/tjae137","url":null,"abstract":"Mosquitoes threaten over half of the world's population through vectored diseases such as malaria, zika, yellow fever, dengue, and chikungunya. Mosquitoes have a highly developed olfactory system attuned to chemotaxis relating to host-seeking, mating, and oviposition behavior. In this study, we aimed to determine the spatial efficacy of 2 plant-based repellent blends (Blend3 and Blend4 that had previously been found to successfully repel Aedes, Anopheles and Culex mosquitoes in wind tunnel assays) in excluding Aedes aegypti from the window entry. A new cage system was developed for parallel \"no-choice\" and \"choice\" olfactometric assays. In the no-choice trial, Blends 3 and 4, as well as commercial products (N, N-diethyl-3-methylbenzamide, p-menthane-3,8-diol [PMD], 3-(N-n-butyl-N-acetyl)-amino-propionic acid ethyl ester, and 2-(2-hydroxyethyl)-1-methylpropylstyrene 1-piperidine carboxylate), were adsorbed into filter papers of different sizes and placed in a window created between 2 attached bug dorms. Then, the number of mosquitoes entering the window was counted through a 6-min period. In choice olfactometric assays, Blends 3, 4, and PMD were adsorbed into filter paper and the number of mosquitoes moving away from Blend 3 and PMD were compared. No-choice assays showed that Blend3 (P < 0.001) and Blend4 (P = 0.0012) were more repellent than the best commercial product PMD. Additionally, while Blend 4 was significantly more repellent than Blend 3 (P = 0.012) in the choice assay, overall, these 2 blends show promise as new repellents for the spatial exclusion of Aedes aegypti from window entry alone or as part of a \"push-pull'' strategy.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"39-46"},"PeriodicalIF":0.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A scoping review to determine if adverse human health effects are associated with use of organophosphates for mosquito control. 进行范围界定审查，以确定使用有机磷控制蚊虫是否会对人类健康产生不利影响。

Journal of medical entomology Pub Date : 2025-01-13 DOI: 10.1093/jme/tjae130

Zoe Tai, C Roxanne Connelly, Suzanne Kuczynski Lange, Nicole Foley, Juan De Leon Rivera, Saul Lozano, Randall J Nett

{"title":"A scoping review to determine if adverse human health effects are associated with use of organophosphates for mosquito control.","authors":"Zoe Tai, C Roxanne Connelly, Suzanne Kuczynski Lange, Nicole Foley, Juan De Leon Rivera, Saul Lozano, Randall J Nett","doi":"10.1093/jme/tjae130","DOIUrl":"10.1093/jme/tjae130","url":null,"abstract":"Organophosphate insecticides are widely used for adult mosquito control. Although proven effective in reducing mosquito populations and limiting arbovirus transmission, public concern exists regarding potential human health effects associated with organophosphate exposure. The aim of this scoping review was to describe any reported human health conditions associated with organophosphates during their use for adult mosquito control in the United States and Canada. Original peer-reviewed articles published in English language journals from 1 January 2000 to 22 May 2024, were obtained by searching from the databases MEDLINE, EMBASE, Agricultural & Environmental Science Collection, CAB Abstracts, and Scopus. The search identified 6,154 screened articles. Following an independent review, 10 studies were identified that described human health conditions associated with organophosphate exposure during adult mosquito control applications. Of the 10 included studies, only two articles were published within the last 11 years (2013 to 22 May 2024). Three types of study design were represented in the included studies: cohort (n = 5), case study (n = 1), and risk assessment (n = 4). The included studies could not determine causality between exposure to adulticides and development of illness or adverse impacts. Exposure to organophosphates did not contribute to an observed increase in metabolic toxicity, hospitalization rates, or self-reported symptoms and exposure. The available and limited evidence indicates that organophosphates can be used safely to control nuisance mosquitoes or mosquitoes that transmit arboviruses. Continued research regarding the human health effects associated with organophosphate applications for adult mosquito control could help evaluate the basis of the public's concerns and inform public health decision-making.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"8-18"},"PeriodicalIF":0.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and age estimation of the intrapuparial period of Phormia regina (Diptera: Calliphoridae) for postmortem interval estimation. Phormia regina（Diptera: Calliphoridae）产卵期的发展和年龄估计，用于死后间隔期估计。

Journal of medical entomology Pub Date : 2025-01-13 DOI: 10.1093/jme/tjae123

Ruonan Zhang, Yinghui Wang, Na Chen, Liangliang Li, Yi Guo, Gengwang Hu, Yundi Gao, Shipeng Shao, Zhiya Gu, Jiangfeng Wang, Yu Wang

引用次数: 0

Genetic characterization of immune adaptor molecule MyD88 in Culex pipiens complex (Diptera: Culicidae) mosquitoes from China. 中国喙库蚊（双翅目：库蚊科）免疫适应分子MyD88的遗传特征。

Journal of medical entomology Pub Date : 2025-01-13 DOI: 10.1093/jme/tjae128

Xueting Wang, Lilan Zhu, Caifei Huo, Dan He, Haifeng Tian, Xiaolan Fan, Yongqing Lyu, Yan Li

{"title":"Genetic characterization of immune adaptor molecule MyD88 in Culex pipiens complex (Diptera: Culicidae) mosquitoes from China.","authors":"Xueting Wang, Lilan Zhu, Caifei Huo, Dan He, Haifeng Tian, Xiaolan Fan, Yongqing Lyu, Yan Li","doi":"10.1093/jme/tjae128","DOIUrl":"10.1093/jme/tjae128","url":null,"abstract":"Mosquitoes of the Culex (Cx.) pipiens complex are vectors of severe diseases including West Nile fever by West Nile virus, Japanese encephalitis by Japanese encephalitis virus, and Lymphatic filariasis by filarial nematode Wuchereria bancrofti. As a major portion of mosquito immune system, the Toll pathway implicates in response against infections of mosquito-borne pathogens and biocontrol agents. The genetic diversity of immune-related molecules is expected to be a feasible and effective introduction to expand our knowledge of the mosquito-microbe interplay. However, a comprehensive description is currently lacking regarding the genetic characteristic of the Toll pathway molecules in Cx. pipiens complex mosquitoes. In the present study, genetic changes in Cx. pipiens complex MyD88 (Myeloid differentiation primary response protein 88) were analyzed as a precedent for the Toll pathway molecules in this taxon. MyD88 is a critical adaptor of the pathway transducing signals from TIR-containing receptors to downstream death domain-containing molecules. Our results revealed that adaptive selection has influenced the genetic changes of the molecule, giving rise to acceleration of diversity at a number of amino acid sites. The adaptively selected sites lie in the death domain, intermediate domain, and C-terminal extension. The characteristics of the genetic changes shed insights into the prominent molecular-level structural basis and the involvement strategy of the adaptor in the arms race against exogenous challenges. This finding would be beneficial for further exploration and deeper understanding of the mosquitoes' vectorial capacity and facilitating the effectiveness and sustainability of the biocontrol agents.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"29-38"},"PeriodicalIF":0.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nocturnal activity and forestry-urban dispersal of phlebotomine sand flies (Diptera: Psychodidae) from an ecotourism park in Amazonian Brazil. 巴西亚马逊生态旅游区沙蝇（双翅目：食蚜蝇科）的夜间活动和森林-城市传播。

Journal of medical entomology Pub Date : 2025-01-13 DOI: 10.1093/jme/tjae120

Danielly Mota Neves, Yetsenia Del Valle Sánchez Uzcátegui, Fernando Tobias Silveira, Thiago Vasconcelos Dos Santos

{"title":"Nocturnal activity and forestry-urban dispersal of phlebotomine sand flies (Diptera: Psychodidae) from an ecotourism park in Amazonian Brazil.","authors":"Danielly Mota Neves, Yetsenia Del Valle Sánchez Uzcátegui, Fernando Tobias Silveira, Thiago Vasconcelos Dos Santos","doi":"10.1093/jme/tjae120","DOIUrl":"10.1093/jme/tjae120","url":null,"abstract":"Phlebotomine sand flies are insects of notorious importance in public health, mainly due to their involvement in the transmission of Leishmania protozoa. Their flight activity occurs predominantly in the twilight/night period, being stimulated mainly by the need to search for food and reproduction. Despite being naturally wild, some species are able to invade anthropized environments. Present work aimed to assess the nocturnal activity and forestry-urban dispersal of phlebotomine sand flies from an ecotourism park in Belém, Amazonian Brazil. The study area comprised a horizontal transect, extending from a forest park to the neighboring urban environment, in Belém. Sampling was conducted with night-operating light traps. Nocturnal activity was assessed through time-set captures with a collection bottle rotator in the forest environment. Dispersal was assessed through captures carried out along the transect, starting from the forest edge (0 m), extending to the urban environment (50-200 m), phlebotomine sand flies were identified. Abundance, richness, diversity, and sampling sufficiency were estimated. Fourteen species were recorded in the surveyed environments, with Nyssomyia antunesi, Trichophoromyia brachipyga, and Trichophoromyia ubiquitalis being the most abundant. Nocturnal activity behavior of Ny. antunesi occurred between 8 PM and 4 AM while that of Th. brachipyga and Th. ubiquitalis occurred between 2 AM and 6 AM. In the urban environment, the 150 m site presented the highest abundance. Bichromomyia flaviscutellata was sampled in all sites. Gravid females of Pressatia choti and Bi. flaviscutellata were sampled in the urban environment. Putative differential activity between the species herein assessed and their urban dispersal observed are worthy of note, adding data for supporting vector surveillance at a local scale.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"112-122"},"PeriodicalIF":0.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142309508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viable Trypanosoma cruzi cultured from a dead Paratriatoma lecticularia (Hemiptera: Reduviidae) encountered in a large dog kennel environment in south Texas, USA. 从美国得克萨斯州南部大型犬舍环境中发现的一只死亡的 Paratriatoma lecticularia（半翅目：Reduviidae）中培养出的克鲁斯 Trypanosoma cruzi。

Journal of medical entomology Pub Date : 2025-01-13 DOI: 10.1093/jme/tjae129

Rachel E Busselman, Keswick C Killets, Ashley B Saunders, Sarah A Hamer

{"title":"Viable Trypanosoma cruzi cultured from a dead Paratriatoma lecticularia (Hemiptera: Reduviidae) encountered in a large dog kennel environment in south Texas, USA.","authors":"Rachel E Busselman, Keswick C Killets, Ashley B Saunders, Sarah A Hamer","doi":"10.1093/jme/tjae129","DOIUrl":"10.1093/jme/tjae129","url":null,"abstract":"Trypanosoma cruzi (Chagas, 1909) is a protozoan parasite transmitted by triatomine (Hemiptera: Reduviidae) insects and is the causative agent of Chagas disease. Oral transmission of the parasite occurs through consumption of contaminated food or infected triatomines and may depend on the degree to which T. cruzi survives in triatomine abdomens. Dead triatomines may be abundant in areas with insecticide use, such as dog kennels where animals may encounter them. We attempted to culture T. cruzi from the gut material of 108 triatomines collected near dog kennels-14 found alive and 94 found dead-and also tested for T. cruzi DNA and discrete typing units using PCR. In total, 30 (27.8%) tested positive for T. cruzi using PCR, 5 alive (35.7%) and 25 dead (26.6%), with no difference in infection between insects found alive versus dead (P-value = 0.53) and more PCR positives identified in dead triatomines with intact gut contents than in dead desiccated triatomines (P-value = 0.049). One Paratriatoma lecticularia (Stål, 1859) that was found dead (1.1%, n = 94) had T. cruzi growth in culture. Given the use of bleach for external decontamination of triatomines as well as the level of bacterial and fungal contamination of cultures, both of which may have impacted the growth of T. cruzi, the apparent prevalence of viable parasites in this study should be interpreted as a conservative estimate. Vector control initiatives should consider that dead insects may still pose a risk of T. cruzi transmission to animals and humans.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":" ","pages":"225-229"},"PeriodicalIF":0.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0