Journal of medical entomology最新文献_第8页

Bacteria in stable fly (Diptera: Muscidae) feces inform foraging decisions of conspecific flies. 稳定蝇（双翅目：蝇科）粪便中的细菌为同种蝇的觅食决策提供信息。

IF 2

Journal of medical entomology Pub Date : 2026-05-05 DOI: 10.1093/jme/tjag084

Emmanuel Hung, Augustus Negraeff, Caelen Watson, Regine Gries, Pantea Zamani, Aryan Monfared, Cyan Luke, Gerhard Gries

{"title":"Bacteria in stable fly (Diptera: Muscidae) feces inform foraging decisions of conspecific flies.","authors":"Emmanuel Hung, Augustus Negraeff, Caelen Watson, Regine Gries, Pantea Zamani, Aryan Monfared, Cyan Luke, Gerhard Gries","doi":"10.1093/jme/tjag084","DOIUrl":"10.1093/jme/tjag084","url":null,"abstract":"Blood-feeding stable flies, Stomoxys calcitrans (L.), are reportedly attracted to conspecific feces. We investigated whether stable flies are attracted to fly feces-derived bacteria, and whether attraction varies with bacterial species and growth substrate, and the sex and reproductive status of foraging flies. We isolated seven bacterial species from fecal deposits of stable flies. In laboratory two-choice bioassays with paired adhesive-coated traps baited with sterile agar or bacteria-inoculated agar, the seven bacterial species in combination repelled flies. However, the bacterial congeners Serratia marcescens and Serratia surfactantfaciens attracted flies when tested singly. Analyses of the headspace volatiles of S. marcescens and S. surfactantfaciens by gas chromatography-mass spectrometry revealed near-identical odor profiles, which may explain their comparable attractiveness to flies. Flies were more attracted to S. marcescens grown on trypticase soy agar than to S. marcescens grown on bovine blood agar, likely due to substrate-dependent odor profiles. Feces-dwelling bacteria appear to function in the context of foraging rather than oviposition given that S. marcescens attracted both female and male stable flies, and both gravid and non-gravid females. That agar plates inoculated with all seven bacterial species failed to attract foraging flies could mean that the microbial community on agar did not sufficiently resemble that in fly feces, perhaps due to the limitations of culture-dependent isolation methods. Alternatively, additional feces-derived odors may also be necessary for fly attraction. Further investigation is warranted to identify the semiochemicals, and possibly other cues, produced by S. marcescens that make this bacterium distinctively attractive to stable flies.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13264395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148255262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of environmental fungi from Crete reveals candidates for biological control of mosquitoes. 克里特岛环境真菌的筛选揭示了生物防治蚊虫的候选物。

IF 2

Journal of medical entomology Pub Date : 2026-05-05 DOI: 10.1093/jme/tjag069

Joel C Couceiro, Martyn J Wood, Juan J Silva, Andronikos Papadopoulos, John Vontas, George Dimopoulos

{"title":"Screening of environmental fungi from Crete reveals candidates for biological control of mosquitoes.","authors":"Joel C Couceiro, Martyn J Wood, Juan J Silva, Andronikos Papadopoulos, John Vontas, George Dimopoulos","doi":"10.1093/jme/tjag069","DOIUrl":"10.1093/jme/tjag069","url":null,"abstract":"Mosquito-borne diseases remain a pressing global health concern, exacerbated by vector range-expansion and insecticide resistance. Entomopathogenic fungi represent a promising alternative to chemical insecticides, yet most mycoinsecticides are based on a few well-studied species. In this study, we surveyed environmental samples from Crete, Greece, isolating 46 isolates representing 13 genera. After a preliminary screening, 8 isolates were selected for larvicidal and adulticidal assays against Culex pipiens molestus Forskål (Diptera: Culicidae). The most promising isolate was BS2-C3 (Metarhizium Sorokin) which achieved over 90% mortality in both assays. Isolates from other genera (eg Cladosporium Link, Penicillium Link, Purpureocillium Luangsa-ard, Hywel-Jones, Houbraken & Samson) also exhibited activity, warranting further investigation. The performance of BS2-C3 was compared with commercially available strains GHA [Beauveria bassiana (Bals.-Criv.) Vuill.] and V275 (Metarhizium brunneum Petch), being as efficient as V275 and more efficient than GHA. These findings demonstrate that, in addition to the well-established genera, other fungal taxa may also harbor mosquitocidal potential, underscoring the importance of continued exploration of fungal diversity for biocontrol. Overall, our results expand the pool of fungi with demonstrated mosquito activity and highlight BS2-C3 (Metarhizium sp.) as a strong candidate for further development into safe, effective, and environmentally sustainable mycoinsecticides for integrated vector management.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13202464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148038755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cattle skin twitching as a behavioral indicator of trap-based stable fly activity under seasonal and thermal conditions. 在季节和热条件下，牛皮肤抽搐作为捕蝇器蝇类稳定活动的行为指标。

IF 2

Journal of medical entomology Pub Date : 2026-05-05 DOI: 10.1093/jme/tjag088

Tatsuo Fujioka, Koshi Asami, Kotaro Akaishi, Haruho Kodama, Kishin Inoue, Kazunori Matsuo

{"title":"Cattle skin twitching as a behavioral indicator of trap-based stable fly activity under seasonal and thermal conditions.","authors":"Tatsuo Fujioka, Koshi Asami, Kotaro Akaishi, Haruho Kodama, Kishin Inoue, Kazunori Matsuo","doi":"10.1093/jme/tjag088","DOIUrl":"https://doi.org/10.1093/jme/tjag088","url":null,"abstract":"The stable fly, Stomoxys calcitrans (Diptera: Muscidae), is a blood-sucking pest of livestock worldwide. Cattle respond to stable fly attacks with evasive behaviors such as skin twitching, foot stamping, and tail flicking. Although such behaviors may be useful indicators of biting fly pressure, their relationship with trap-based stable fly activity across seasons remains unclear. We conducted year-round hourly observations of stable fly flight activity and cattle skin twitching in a dairy barn in Fukuoka, Japan. Stable fly flight activity was monitored using blue sticky traps, and cattle skin twitching was recorded simultaneously. Model-based analyses showed that stable fly flight activity was concentrated during daytime, varied seasonally, and had a nonlinear relationship with air temperature. The predicted maximum daytime flight activity occurred at 26.7 °C. Cattle skin twitch frequency was associated with stable fly activity and air temperature. These results suggest that cattle skin twitching may serve as a practical supplementary indicator of stable fly activity, although its interpretation should account for seasonal and thermal conditions.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148267555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal flooding and environmental variation decrease blacklegged tick (Acari: Ixodidae; Ixodes scapularis) survival in a wetland ecosystem. 季节性洪水和环境变化降低了湿地生态系统中黑腿蜱（蜱螨科）的生存。

IF 2

Journal of medical entomology Pub Date : 2026-05-05 DOI: 10.1093/jme/tjag103

Ningzhu Bai, Afsoon Sabet, Allison Williams, Risa Pesapane

{"title":"Seasonal flooding and environmental variation decrease blacklegged tick (Acari: Ixodidae; Ixodes scapularis) survival in a wetland ecosystem.","authors":"Ningzhu Bai, Afsoon Sabet, Allison Williams, Risa Pesapane","doi":"10.1093/jme/tjag103","DOIUrl":"https://doi.org/10.1093/jme/tjag103","url":null,"abstract":"Increased volatility in precipitation can result in higher levels of rainfall and contribute to flooding events. Ticks spend the majority of their life in the environment, and this volatility may impact their survival and long-term occupancy in certain habitat types. While underwater survival has been assessed in some tick species, the effect of water inundation on blacklegged ticks (Ixodes scapularis Say) is not well understood. Here, we elucidated the survival of blacklegged tick nymphs in a wetland ecosystem by creating microcosms called tickaria (n = 48) and deploying these tickaria in \"dry\" and \"wet\" sites within an experimental wetland research station in Ohio. Nymphs (n = 10) were contained for the duration of the nymphal season in Ohio, and tickaria were destructively sampled periodically between May and August to assess survival. We found that ticks in the wet sites that experienced flooding had a higher risk of death compared to ticks in dry sites without flooding (P < 0.001). High volumetric water content in soil also contributed to a higher risk of death (P < 0.001). This work suggests that blacklegged tick nymphs are unable to withstand flooding events or saturated soil for long periods of time and expands our understanding of tick survival while inundated in an ecologically relevant system.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructure of mouthpart and terminalia in Musca crassirostris (Diptera: Muscidae) with molecular confirmation from Thailand. 泰国白蝇（双翅目：蝇科）口器和末端的超微结构及其分子鉴定。

IF 2

Journal of medical entomology Pub Date : 2026-05-05 DOI: 10.1093/jme/tjag097

Ketsarin Thipphet, Sangob Sanit, Kwankamol Limsopatham, Sukanya Horpaopan, Tarinee Chaiwong, Kittikhun Moophayak, Apichat Vitta, Pluemkamon Phuwanatsarunya, Jidapa Kunlanantakun, Kanyakon Watanasak, Mukmanee Photo, Wanida Kanta, Hiromu Kurahashi, Kabkaew L Sukontason, Nophawan Bunchu

{"title":"Ultrastructure of mouthpart and terminalia in Musca crassirostris (Diptera: Muscidae) with molecular confirmation from Thailand.","authors":"Ketsarin Thipphet, Sangob Sanit, Kwankamol Limsopatham, Sukanya Horpaopan, Tarinee Chaiwong, Kittikhun Moophayak, Apichat Vitta, Pluemkamon Phuwanatsarunya, Jidapa Kunlanantakun, Kanyakon Watanasak, Mukmanee Photo, Wanida Kanta, Hiromu Kurahashi, Kabkaew L Sukontason, Nophawan Bunchu","doi":"10.1093/jme/tjag097","DOIUrl":"10.1093/jme/tjag097","url":null,"abstract":"Musca crassirostris Stein, 1903 (Diptera: Muscidae) is a medically and veterinary important hematophagous fly whose feeding activity causes significant irritation and blood loss in livestock and is occasionally associated with human myiasis. Although this species is widely distributed and epidemiologically relevant, its feeding-related functional morphology and genital diagnostic characters have remained insufficiently documented at the ultrastructural level. Here, we present the first comprehensive scanning electron microscopy (SEM)-based characterization of the labellar feeding apparatus and male and female terminalia of M. crassirostris, integrating stereomicroscopy and mitochondrial cytochrome c oxidase subunit I (COI) DNA barcoding for species confirmation. SEM images revealed well-developed prestomal teeth and serrated pseudotracheal structures, features directly associated with hematophagous feeding. Distinctive genital characters are also present and facilitate accurate species identification. The COI barcoding confirmed species identity with complete concordance among BOLD, GenBank, and species delimitation analyses, providing new reference data for Southeast Asian populations. By linking ultrastructural morphology with feeding function and diagnostic utility, this study establishes a robust morphological-molecular framework that advances current understanding of M. crassirostris and supports its identification and surveillance in medical and veterinary entomology.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148335226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Household insecticide use and metabolic resistance mechanisms in Anopheles gambiae s.l. from Central-Western and Eastern Côte d'Ivoire. Côte科特迪瓦中西部和东部冈比亚按蚊的家庭杀虫剂使用和代谢抗性机制。

IF 2

Journal of medical entomology Pub Date : 2026-05-05 DOI: 10.1093/jme/tjag064

Armand Kouassi Ekra, Constant Victorien Ako Edi, Christabelle Gba Sadia, Romaric Landry Gballet, France Paraudie Kouadio, Kouadio Fodjo Behi, Assamoi Jean-Baptiste, Danho Mathias, Benjamin Guibehi Koudou

{"title":"Household insecticide use and metabolic resistance mechanisms in Anopheles gambiae s.l. from Central-Western and Eastern Côte d'Ivoire.","authors":"Armand Kouassi Ekra, Constant Victorien Ako Edi, Christabelle Gba Sadia, Romaric Landry Gballet, France Paraudie Kouadio, Kouadio Fodjo Behi, Assamoi Jean-Baptiste, Danho Mathias, Benjamin Guibehi Koudou","doi":"10.1093/jme/tjag064","DOIUrl":"10.1093/jme/tjag064","url":null,"abstract":"The widespread use of household insecticides represents a potential but often overlooked source of selection pressure contributing to insecticide resistance in malaria vectors. In Côte d'Ivoire, resistance to pyrethroids is widespread; however, the role of domestic insecticide practices in shaping metabolic resistance mechanisms remains poorly documented. A cross-sectional household survey was conducted in 3 urban localities of Côte d'Ivoire (Abengourou, Daloa, and Gagnoa) to characterize insecticide use patterns, frequencies, and application practices. In parallel, larvae of Anopheles gambiae s.l. were collected from the same localities, reared to adulthood, and analyzed for the expression of 8 detoxification genes (CYP6P3, CYP6M2, CYP9K1, CYP6P4, CYP6Z1, GSTE2, CYP6P1, and CYP4G16) using quantitative real-time PCR. Gene expression levels were compared with those of the insecticide-susceptible reference strain Anopheles gambiae Kisumu using REST software. Overall, 63.0% of households reported regular use of insecticides, with marked heterogeneity between sites Pyrethroid-based formulations, predominantly permethrin-transfluthrin combinations, were the most commonly used products. Household insecticide consumption was highest in Abengourou, exceeding 2 aerosol cans (300 ml) per week. Molecular analyses revealed significant overexpression of CYP6P1 in An. gambiae s.l. from Abengourou and CYP6Z1 in populations from Daloa and Gagnoa compared with the Kisumu reference strain. The intensive and recurrent use of household insecticides is associated with the presence of metabolic resistance markers; however, causality cannot be established. These findings highlight the need to integrate domestic insecticide practices into national insecticides resistance management strategies.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative taxonomy reveals a new species of black fly (Diptera: Simuliidae) in the Simulium varicorne species-group from Thailand. 综合分类揭示了泰国黑蝇种群一新种（双翅目：黑蝇科）。

IF 2

Journal of medical entomology Pub Date : 2026-05-05 DOI: 10.1093/jme/tjag062

Kittipat Aupalee, Wichai Srisuka, Kevin Charoenvilaisiri, Wanchai Maleewong, Atiporn Saeung, Hiroyuki Takaoka

{"title":"Integrative taxonomy reveals a new species of black fly (Diptera: Simuliidae) in the Simulium varicorne species-group from Thailand.","authors":"Kittipat Aupalee, Wichai Srisuka, Kevin Charoenvilaisiri, Wanchai Maleewong, Atiporn Saeung, Hiroyuki Takaoka","doi":"10.1093/jme/tjag062","DOIUrl":"10.1093/jme/tjag062","url":null,"abstract":"Black flies are a group of hematophagous insects with high species diversity, driven by a combination of ecological, evolutionary, and life-history factors. By integrating DNA barcoding with traditional morphological method, a new black fly species was discovered. Simulium (Gomphostilbia) wiangpingense sp. nov. is described based on females, males, pupae, and mature larvae collected from Chiang Mai province, Thailand. This new species is assigned to the S. burtoni subgroup of the S. varicorne species-group within the subgenus Gomphostilbia Enderlein, 1921. It is characterized in the female and male adults by the antennae with eight flagellomeres, and pleural membrane bare; in the female by the sensory vesicle elongate, 0.62 to 0.70 times length of the third palpal segment, mandible without teeth on the outer margin, and wing with the subcosta haired; in the male by the sensory vesicle ellipsoidal, 0.42 to 0.43 times length of the third palpal segment; in the pupa by the gill with eight filaments arranged as 3 + 3 + 2 from dorsal to ventral; and in the larva by the long postgenal cleft. Morphologically and genetically, the new species is most closely related to S. burtoni Takaoka & Davies, 1995. Phylogenetic analysis and species delimitation clearly distinguish the new species from its congeners, providing additional support for its recognition as a distinct species. Taxonomic notes for distinguishing the new species from related species within the S. burtoni subgroup from Thailand and other countries, and identification keys to all the 16 species of the S. varicorne species-group are provided.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147966250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Susceptibility of secondary screwworm (Diptera: Calliphoridae) to the larval parasitoid Tachinaephagus zealandicus (Hymenoptera: Encyrtidae). 次生螺旋蝇（双翅目：小锥蝇科）对新西兰锥蝇幼虫（膜翅目：小锥蝇科）的敏感性。

IF 2

Journal of medical entomology Pub Date : 2026-05-05 DOI: 10.1093/jme/tjag087

Christopher J Geden, Alex P Arp, Paul V Hickner, Mackenzie Tietjen, Alexandra A Pagac

{"title":"Susceptibility of secondary screwworm (Diptera: Calliphoridae) to the larval parasitoid Tachinaephagus zealandicus (Hymenoptera: Encyrtidae).","authors":"Christopher J Geden, Alex P Arp, Paul V Hickner, Mackenzie Tietjen, Alexandra A Pagac","doi":"10.1093/jme/tjag087","DOIUrl":"10.1093/jme/tjag087","url":null,"abstract":"The reinfestation of New World screwworm (Cochliomyia hominivorax [Coquerel]) (Diptera: Calliphoridae) in North America has prompted a broad effort to identify new management tools. In this study, the larval parasitoid Tachinaephagus zealandicus (Ashmead) (Hymenoptera: Encyrtidae) was evaluated as a potential biocontrol using the congeneric secondary screwworm Cochliomyia macellaria (Fab.) (Diptera: Calliphoridae) as a proxy host. When host:parasitoid ratios were varied from 5:1 to 40:1, female parasitoids killed up to 18 hosts (at 40:1) and produced 66 adult wasp progeny per female over 24 h. Parasitized pupae produced from 5.6 to 11.7 adult parasitoids per pupa at host:parasitoid ratios of 40:1 and 5:1, respectively. Parasitoids showed no preference for larvae of C. macellaria or Sarcophaga bullata Parker (Diptera: Sarcophagidae). Kill rates and successful parasitism was twice as high in moist (15% moisture) as in dry sandy loam soil. Addition of a layer of leaf litter to the soil surface had no effect on the ability of parasitoids to locate hosts. Parasitism in moist clay soil was similar to that in moist sandy soil, but higher parasitism was observed in dry clay than on dry sandy soil. Placing faux rocks as potential larval refuge on the pupation substrate surface provided larvae with some protection from parasitism in dry substrates. When larvae were given a 2-h head start before adding parasitoids, there was no effect on host mortality or parasitism in dry soil. Results are encouraging for possible use of T. zealandicus as a supplemental management tool for calliphorid pests.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148207273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Chlorella sorokiniana KU.B2 microalga on the development of Culex quinquefasciatus (Diptera: Culicidae) larvae. 小球藻KU的作用。微藻对致倦库蚊（双翅目：库蚊科）幼虫发育的影响。

IF 2

Journal of medical entomology Pub Date : 2026-05-05 DOI: 10.1093/jme/tjag089

Jemisha Dudhat, Jassada Saingamsook, Baby Kyi Soe, Nuttha Sanevas, Saowalak Kaewmee, Chonlada Mano, Catherine Walton, Padet Siriyasatien, George Dimopoulos, Narissara Jariyapan

{"title":"Effects of Chlorella sorokiniana KU.B2 microalga on the development of Culex quinquefasciatus (Diptera: Culicidae) larvae.","authors":"Jemisha Dudhat, Jassada Saingamsook, Baby Kyi Soe, Nuttha Sanevas, Saowalak Kaewmee, Chonlada Mano, Catherine Walton, Padet Siriyasatien, George Dimopoulos, Narissara Jariyapan","doi":"10.1093/jme/tjag089","DOIUrl":"https://doi.org/10.1093/jme/tjag089","url":null,"abstract":"Culex quinquefasciatus (Say) is a significant vector of lymphatic filariasis and arboviruses. Current control strategies rely heavily on chemical insecticides, highlighting the need for alternative and environmentally sustainable approaches. This study evaluated the effects of the microalga Chlorella sorokiniana KU.B2 on the development of Cx. quinquefasciatus larvae. Larvae were reared on microalgal-rabbit food diets: a control (100% rabbit food) and four treatments with increasing proportions of C. sorokiniana suspension (25 microalgae [Al], 50Al, 75Al, and 100Al). Additional rabbit food-only controls with matched food proportions were included to distinguish microalgal effects from reduced food availability. Larval growth, pupation, adult emergence, and mortality were assessed, and gut histology and ultrastructure were examined descriptively. Higher proportions of microalgae (75Al and 100Al) were associated with failure of pupation and adult emergence, resulting in complete larval mortality. Lower proportions (25Al and 50Al) caused delayed development and reduced survival compared with controls. Larvae provided reduced amounts of rabbit food alone were still able to complete development, indicating that the observed effects were associated with microalgal inclusion rather than reduced food availability alone. Histological and ultrastructural observations revealed numerous intact and partially degraded microalgal cells within the larval gut, while recovery of viable cells after gut passage suggested that some ingested cells remained viable after transit through the larval digestive tract. Overall, C. sorokiniana KU.B2 negatively affected the development and survival of Cx. quinquefasciatus larvae in a dose-dependent manner. Further investigation into the mechanisms underlying these effects and their ecological relevance is needed.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148255297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiple genetic lineages of the invasive brown widow spider (Araneae: Theridiidae) from inland areas in the Kanto region, Japan. 日本关东内陆地区入侵褐寡妇蜘蛛（蜘蛛目：蜘蛛科）的多重遗传谱系。

IF 2

Journal of medical entomology Pub Date : 2026-05-05 DOI: 10.1093/jme/tjag077

Hirotaka Katahira, Syu Tsutsui, Sana Kishino, Takumi Tsujino, Hiroki Yajima, Yuusuke Sawada, Yuya Eguchi, Takahiro Horiguchi, Rei Hirasawa, Daisuke Hayasaka, Kozue Nitta

{"title":"Multiple genetic lineages of the invasive brown widow spider (Araneae: Theridiidae) from inland areas in the Kanto region, Japan.","authors":"Hirotaka Katahira, Syu Tsutsui, Sana Kishino, Takumi Tsujino, Hiroki Yajima, Yuusuke Sawada, Yuya Eguchi, Takahiro Horiguchi, Rei Hirasawa, Daisuke Hayasaka, Kozue Nitta","doi":"10.1093/jme/tjag077","DOIUrl":"10.1093/jme/tjag077","url":null,"abstract":"The brown widow spider, Latrodectus geometricus Koch, 1841, is a medically important invasive species that possesses neurotoxic venom capable of causing latrodectism in humans. This species has recently been found in inland areas of the Kanto region in Japan, raising concerns about its ongoing range expansion and associated public health risks. Here, we analyzed partial sequences of mitochondrial cytochrome c oxidase subunit I (cox1) and 16S ribosomal DNA from 99 individuals (3 males and 96 females) collected from multiple sites in Tokyo and Kanagawa prefectures, evaluating their phylogenetic positions and introduction history. The analysis revealed two major lineages, with six cox1 haplotypes (LGc1-LGc6) and five 16S rDNA haplotypes (LGs1-LGs5). One lineage comprised previously unreported basal haplotypes, including LGc5 and LGc6 in cox1 and LGs5 in 16S rDNA. The other lineage included four haplotypes (LGc1-LGc4 and LGs1-LGs4), which have been reported from South Africa and various invaded regions globally. In Sagamihara City (Kanagawa), where numerous individuals were consistently detected in previous surveys, both lineages co-occurred, suggesting multiple introductions and/or high propagule pressure. To clarify the dispersal routes and source populations of each lineage and haplotype, more comprehensive genetic data from both its native range in Africa and globally established populations are needed.","PeriodicalId":94091,"journal":{"name":"Journal of medical entomology","volume":"63 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148177245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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