Journal of economic entomology最新文献_第3页

Applying a computer model to evaluate the evolution of resistance by western corn rootworm to multiple Bt traits in transgenic maize. 应用计算机模型评估西部玉米根虫对转基因玉米中多种 Bt 性状的抗性演变。

Journal of economic entomology Pub Date : 2024-11-05 DOI: 10.1093/jee/toae260

John B McCulloch, Aaron J Gassmann

{"title":"Applying a computer model to evaluate the evolution of resistance by western corn rootworm to multiple Bt traits in transgenic maize.","authors":"John B McCulloch, Aaron J Gassmann","doi":"10.1093/jee/toae260","DOIUrl":"https://doi.org/10.1093/jee/toae260","url":null,"abstract":"Western corn rootworm, Diabrotica virgifera virgifera (LeConte) (Coleoptera: Chrysomelidae), is a major pest of maize in the United States. Transgenic maize producing insecticidal toxins from the bacterium Bacillus thuringiensis (Bt) have been used to manage this pest since 2003. Refuges of non-Bt maize have been used to delay resistance to Bt maize by western corn rootworm, and are planted in conjunction with maize producing single or multiple (i.e., pyramids) Bt toxins. Two Bt toxins, Cry3Bb1 and Gpp34/Tpp35Ab1, were used individually before being combined as a pyramid, at which point resistance had already evolved to Cry3Bb1. Pyramids targeting western corn rootworm therefore contained at least one toxin to which resistance had evolved. Western corn rootworm has now evolved resistance to all four commercially available Bt toxins used for rootworm management. We used laboratory and field-generated data to parameterize a deterministic model to simulate the effectiveness of refuges and Bt pyramids to delay resistance to Bt maize in western corn rootworm. Resistance to the pyramid of Cry3Bb1 with Gpp34/Tpp35Ab1 evolved more rapidly when resistance to Cry3Bb1 was already present. This effect arose when model conditions affecting initial resistance allele frequency, inheritance of resistance, and fitness costs were varied. Generally, resistance evolved faster when initial resistance allele frequencies were higher, inheritance of resistance was nonrecessive, and fitness costs were absent, which is consistent with previous models that simulated resistance evolution. We conclude that new transgenic pyramids should pair novel, independently acting toxins with abundant refuges to minimize the risk of rapid resistance evolution.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial communities in Asecodes hispinarum (Hymenoptera: Eulophidae) and its host Brontispa longissima (Coleoptera: Chrysomelidae), with comparison of Wolbachia dominance. Asecodes hispinarum (Hymenoptera: Eulophidae) 及其宿主 Brontispa longissima (Coleoptera: Chrysomelidae) 的细菌群落，以及 Wolbachia 优势比较。

Journal of economic entomology Pub Date : 2024-11-04 DOI: 10.1093/jee/toae234

Sheng-Yuan Yang, Yu-Ying Lin, Zheng Hao, Zi-Jie Li, Zheng-Qiang Peng, Tao Jin

{"title":"Bacterial communities in Asecodes hispinarum (Hymenoptera: Eulophidae) and its host Brontispa longissima (Coleoptera: Chrysomelidae), with comparison of Wolbachia dominance.","authors":"Sheng-Yuan Yang, Yu-Ying Lin, Zheng Hao, Zi-Jie Li, Zheng-Qiang Peng, Tao Jin","doi":"10.1093/jee/toae234","DOIUrl":"https://doi.org/10.1093/jee/toae234","url":null,"abstract":"The endoparasitoid Asecodes hispinarum (Bouček) (Hymenoptera: Eulophidae) serves as an effective biological control agent against Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae), a notorious palm pest. Endosymbionts found in parasitoids and their hosts have attracted significant attention due to their substantial influence on biocontrol efficacy. In this study, we employed 16S rRNA sequencing, polymerase chain reaction, and fluorescence in situ hybridization to assess the symbiotic bacteria composition, diversity, phylogeny, and localization in A. hispinarum and its host B. longissima. Our findings showed significant differences in the richness, diversity, and composition of symbiotic bacteria among different life stages of B. longissima. Notably, the bacterial richness, diversity, and composition of A. hispinarum was similar to that of B. longissima. Firmicutes and Proteobacteria were the dominant phyla, while Wolbachia was the dominant genera across the parasitoid and host. It was discovered for the first time that Wolbachia was present in A. hispinarum with a high infection rate at ≥ 96.67%. Notably, the Wolbachia strain in A. hispinarum was placed in supergroup A, whereas it was categorized under supergroup B in B. longissima. Furthermore, Wolbachia is concentrated in the abdomen of A. hispinarum, with particularly high levels observed in the ovipositors of female adults. These findings highlight the composition and diversity of symbiotic bacteria in both A. hispinarum and its host B. longissima, providing a foundation for the development of population regulation strategies targeting B. longissima.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A larval expressed chemosensory protein involved in recognition of anthocyanins in Spodoptera litura (Lepidoptera: Noctuidae). 一种幼虫表达的化学感知蛋白参与了 Spodoptera litura（鳞翅目：夜蛾科）花青素的识别。

Journal of economic entomology Pub Date : 2024-11-01 DOI: 10.1093/jee/toae239

Xiaolin Dong, Yaling Huang, Yanfang Pei, Ling Chen, Tianliang Tan, Fayun Xiang, Chuanren Li, Lijun Fu

{"title":"A larval expressed chemosensory protein involved in recognition of anthocyanins in Spodoptera litura (Lepidoptera: Noctuidae).","authors":"Xiaolin Dong, Yaling Huang, Yanfang Pei, Ling Chen, Tianliang Tan, Fayun Xiang, Chuanren Li, Lijun Fu","doi":"10.1093/jee/toae239","DOIUrl":"https://doi.org/10.1093/jee/toae239","url":null,"abstract":"Anthocyanins are secondary metabolites which act as diverse functions during plant growth. Insects can discriminate host plants by their sensitive gustatory systems. It is hypothetical that chemosensory proteins (CSPs) play a crucial role in regulating this behavioral process. However, the underlying molecular mechanisms remain obscure. In the present study, we characterized a CSP SlitCSP8 from the Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Quantitative real-time-polymerase chain reaction analysis demonstrated that SlitCSP8 was mainly expressed in the head of the 7th S. litura larvae, especially labrum. Further, recombinant SlitCSP8 was obtained using bacterial expression system. Fluorescence competitive binding assays demonstrated that the purified SlitCSP8 exhibited a strong binding affinity to anthocyanins, a natural compound derived from the host plant. Silencing SlitCSP8 through RNAi significantly reduced the sensitivity of S. litura larvae to anthocyanins-treated leaf disks, the development from larva to pupae was not affected. These data provide insight into the molecular basis that CSP8 can detect anthocyanins in host plants by chemosensory system of insects. It can be further used in designing novel optimal food attractant targeting to the CSPs for pest control.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring and management of the Nearctic leafhopper Scaphoideus titanus (Hemiptera: Cicadellidae) in Italian vineyards. 意大利葡萄园中近地叶蝉 Scaphoideus titanus（半翅目：蝉科）的监测与管理。

Journal of economic entomology Pub Date : 2024-11-01 DOI: 10.1093/jee/toae250

Bruno Bagnoli, Renato Ricciardi, Livia De Fazi, Giordana D'Anna, Piero Braccini, Giovanni Benelli, Andrea Lucchi

{"title":"Monitoring and management of the Nearctic leafhopper Scaphoideus titanus (Hemiptera: Cicadellidae) in Italian vineyards.","authors":"Bruno Bagnoli, Renato Ricciardi, Livia De Fazi, Giordana D'Anna, Piero Braccini, Giovanni Benelli, Andrea Lucchi","doi":"10.1093/jee/toae250","DOIUrl":"https://doi.org/10.1093/jee/toae250","url":null,"abstract":"The Nearctic leafhopper Scaphoideus titanus Ball (Hemiptera: Cicadellidae) is the main vector of the grapevine flavescence dorée (FD) phytoplasmas in Europe. To limit the impact of pest control activities on human health and the environment, EU legislation is withdrawing a growing number of active ingredients (a.i.), highlighting the need to evaluate alternative S. titanus management tools. In this context, we carried out a three-year (2021-2023) study in Italian vineyards characterized by high S. titanus populations. We compared the effectiveness of different insecticide-based strategies, i.e., targeting only nymphs (2021) or nymphs and adults (2022-2023), by spraying some of the currently allowed products (i.e., flupyradifurone, acetamiprid, sulfoxaflor, etofenprox, deltamethrin, and pyrethrins). The effectiveness of insecticide-based treatments was evaluated in terms of adult catches on yellow sticky traps. Furthermore, to achieve useful insights for optimizing current monitoring tools, we compared S. titanus catches on sticky traps of five different colors (i.e., yellow, green, blue, black, and white), as well as those achieved with two types of currently marketed yellow sticky traps. Results showed that etofenprox, deltamethrin, and sulfoxaflor were the most effective products for reducing S. titanus adult populations (33.64%, 30.30%, and 31.26%, respectively). Yellow and green sticky traps were more attractive to S. titanus adults than white, blue, and black ones. Moreover, a significant difference in capture efficiency was observed between two commercial yellow devices (i.e., tenfold higher catches using Glutor over Super color). Practical implications of our results are discussed in the context of current IPM against this key leafhopper vector of FD.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of insecticide resistance and their mechanisms in field populations of the German cockroach (Blattodea: Ectobiidae) in Taiwan under different treatment regimes. 台湾德国小蠊（Blattodea: Ectobiidae）田间种群在不同处理制度下的杀虫剂抗药性特征及其机制。

Journal of economic entomology Pub Date : 2024-11-01 DOI: 10.1093/jee/toae252

Panida Kruaysawat, Mei-Er Chen, Shao-Hung Lee, Chow-Yang Lee, Kok-Boon Neoh

{"title":"Characterization of insecticide resistance and their mechanisms in field populations of the German cockroach (Blattodea: Ectobiidae) in Taiwan under different treatment regimes.","authors":"Panida Kruaysawat, Mei-Er Chen, Shao-Hung Lee, Chow-Yang Lee, Kok-Boon Neoh","doi":"10.1093/jee/toae252","DOIUrl":"https://doi.org/10.1093/jee/toae252","url":null,"abstract":"This study investigated how management strategies influence resistance profiles in German cockroach (Blattella germanica (L.)) populations and their impact on the performance of commercial gel baits containing fipronil, imidacloprid, and indoxacarb. Field populations from premises managed under 3 different strategies: Baiting, random insecticide (RI) used, and insecticide rotation (IR) were tested. Almost all populations under RI and IR were resistant to deltamethrin, but low to moderate resistance was observed under the Baiting approach. Cytochrome P450 monooxygenases (P450) were involved in deltamethrin resistance in these resistant populations. All individuals under Baiting and RI were homozygous for the L993F mutation, but the populations under IR lacked homozygous-resistant individuals. Eighty-three percent of field populations with complete homozygosity for the Rdl mutation displayed low mortality upon exposure to 3× LD95 fipronil. The effect of P450 and the Rdl mutation conferred high fipronil resistance in populations under the Baiting approach, recording moderate performance indices (PI) of 44-67 in fipronil bait. By contrast, those populations under RI and IR, in which involve glutathione S-transferases in fipronil resistance, had high PIs of 78-93. Almost 80% of populations exhibited over 90% mortality at 3× LD95 indoxacarb treatment, accompanied by high PIs of 90-100 in indoxacarb bait. Partial mortality from 1× LD95 imidacloprid occurred across all field populations due to the involvement of P450. PIs of imidacloprid bait ranged 5-57 and 20-94 in populations under RI and IR, respectively. Field populations demonstrate different resistance profiles depending on the treatment regimes, and the resistance mechanisms involved influenced gel bait's effectiveness.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection, inheritance, life-history traits, and flight capacity of Grapholita molesta (Lepidoptera: Tortricidae) resistant to lambda-cyhalothrin. 抗溴氰菊酯的 Grapholita molesta（鳞翅目：蓟马科）的选择、遗传、生活史特征和飞行能力。

Journal of economic entomology Pub Date : 2024-11-01 DOI: 10.1093/jee/toae247

Xiaohe Zhang, Sha Su, Chengzhi Jian, Baojian Huang, Jilong Zhang, Jinbo Suo, Xiong Peng, Maohua Chen

{"title":"Selection, inheritance, life-history traits, and flight capacity of Grapholita molesta (Lepidoptera: Tortricidae) resistant to lambda-cyhalothrin.","authors":"Xiaohe Zhang, Sha Su, Chengzhi Jian, Baojian Huang, Jilong Zhang, Jinbo Suo, Xiong Peng, Maohua Chen","doi":"10.1093/jee/toae247","DOIUrl":"https://doi.org/10.1093/jee/toae247","url":null,"abstract":"Grapholita molesta (Busck) (Lepidoptera: Tortricidae) is a globally significant fruit pest traditionally managed using chemical insecticides such as lambda-cyhalothrin. However, repeated applications have led to the development of resistance in field populations. The mechanisms and fitness costs associated with lambda-cyhalothrin resistance in G. molesta remain poorly understood. In this study, we established a lambda-cyhalothrin-resistant strain (RS) of G. molesta through continuous selection with lambda-cyhalothrin in the laboratory for 21 generations, resulting in a 249.6-fold increase in resistance. Bioassay data from the F1 progeny (F1RS and F1SR) of reciprocal crosses between RS and susceptible strain (SS) showed similar degrees of dominance (0.40 for F1RS and 0.32 for F1SR). Significant differences between observed and expected mortalities of backcross individuals suggested that resistance to lambda-cyhalothrin is polygenic. Compared to the SS strain, the RS strain exhibited significantly prolonged periods of larval, prepupal, and pupal stages, as well as a significant decrease in larval weight. In addition, the RS strain showed significant reductions in fecundity, cumulative flight distance, maximum flight distance, cumulative flight time, maximum flight time, and average flight speed. These results suggest that resistance to lambda-cyhalothrin in G. molesta is autosomal, incompletely dominant, and polygenic. Life-history traits such as fecundity and flight capacity were significantly reduced in RS strains compared with the SS strain. These findings provide valuable insights for mitigating lambda-cyhalothrin resistance development and promoting sustainable control of G. molesta in the field.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of different rearing methods on cocoon silk strength in silkworm Bombyx mori (Lepidoptera: Bombycidae). 不同饲养方法对家蚕（鳞翅目：蚕科）茧丝强度的影响

Journal of economic entomology Pub Date : 2024-10-30 DOI: 10.1093/jee/toae242

Shengxiang Zhang, Ran Liu, Fengjie Lan, Yi Wang, Huizhen Wang, Yangyang Liu, Chunjiu Ren, Huiju Gao

{"title":"Effects of different rearing methods on cocoon silk strength in silkworm Bombyx mori (Lepidoptera: Bombycidae).","authors":"Shengxiang Zhang, Ran Liu, Fengjie Lan, Yi Wang, Huizhen Wang, Yangyang Liu, Chunjiu Ren, Huiju Gao","doi":"10.1093/jee/toae242","DOIUrl":"https://doi.org/10.1093/jee/toae242","url":null,"abstract":"In recent years, the use of artificial diet to rear silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae) has advanced rapidly in China. However, significant differences are found in the production and performance of silk from silkworms reared on artificial diet and mulberry leaves, thereby affecting the development of artificial diet usage in sericulture. To understand the reasons for these differences, we tested the following 3 rearing methods: all-instar mulberry leaf rearing (Mul), all-instar artificial diet rearing (Diet), and instars 1-3 reared on artificial diet followed by instars 4-5 reared on mulberry leaf (Mix). The results showed that the silk production was significantly lower under Diet than Mix and Mul. Electron microscopy images revealed that the protein synthesis and energy supply were decreased under Dier and Mix compared with Mul. Subsequent strength analysis indicated that the relative strength of silk was highest under Mix, followed by Mul, and weakest under Diet. However, no significant differences in elongation were observed among treatments. The β-sheet content of silk was significantly higher under Mix than Diet and Mul, and a similar trend was observed for the crystallinity. Furthermore, the elevated expression of BmChiNAG and the reduced expression of BmTpn genes may be a significant factor for the notable disparities in cocoon silk fineness and strength among the threes. These findings provide deep insights into the differences in silk produced by silkworms reared on mulberry leaves and artificial diet, as well as providing a reference for improving artificial diet for rearing silkworms.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subterranean termites (Coptotermes formosanus [Blattodea: Rhinotermitidae]) colonies can readily intercept commercial inground bait stations placed at label-prescribed distance. 地下白蚁（Coptotermes formosanus [Blattodea: Rhinotermitidae]）群落很容易拦截按标签规定距离放置的商用地下诱饵站。

Journal of economic entomology Pub Date : 2024-10-29 DOI: 10.1093/jee/toae259

Kaitlin Gazdick, Sang-Bin Lee, Nobuaki Mizumoto, Thomas Chouvenc, Nan-Yao Su

{"title":"Subterranean termites (Coptotermes formosanus [Blattodea: Rhinotermitidae]) colonies can readily intercept commercial inground bait stations placed at label-prescribed distance.","authors":"Kaitlin Gazdick, Sang-Bin Lee, Nobuaki Mizumoto, Thomas Chouvenc, Nan-Yao Su","doi":"10.1093/jee/toae259","DOIUrl":"https://doi.org/10.1093/jee/toae259","url":null,"abstract":"The Formosan subterranean termite, Coptotermes formosanus Shiraki, is both an economically impactful pest and a successful invader. One method of subterranean termite control is baiting. According to the label, baits are installed surrounding the structure at a uniform interval distance of ≈3 m. However, homeowners and pest control professionals are often concerned that termites may bypass bait stations and have access to the structure. To address this concern, we experimentally duplicated field conditions using a large planar arena (3.6 m × 1.1 m) to study the optimal distribution of bait stations based on colony-wide foraging activity. We installed 2 bait stations 3 m apart as per label instructions and introduced C. formosanus colonies to allow them to explore the arena by tunneling through the sand. In this real-scale arena, all termite colonies intercepted a bait station in an average of 21 (± 8 SD) days. We assumed that termites could find bait faster if there were more bait stations by overlaying additional hypothetical baits closer than per label instruction, but the improvement was incremental, requiring 4 times more stations (0.45 m interval) to obtain a significant difference. We also revealed the characteristic behavior after intercepting bait stations, termites created a burst of tunnels that radiated from the bait station. These branching tunnels averaged 16 cm in length, suggesting immediate interceptions of additional auxiliary stations placed within 16 cm of an active station. These findings contribute to our understanding on how subterranean termites intercept inground bait stations.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosome-level genome of Osmia excavata (Hymenoptera: Megachilidae) provides insights into low-temperature tolerance of Osmia pollinator. 挖掘茭白（膜翅目：Megachilidae）染色体水平的基因组为了解茭白授粉昆虫的低温耐受性提供了线索。

Journal of economic entomology Pub Date : 2024-10-29 DOI: 10.1093/jee/toae257

Fang Ouyang, Honggang Guo, Wen Xie, Yingying Song, Zhuo Yan, Zhenqing Peng, Yongsheng Zhang, Ritao Qu, Huaigen Xin, Zheming Yuan, Zhishu Xiao, Xingyuan Men

{"title":"Chromosome-level genome of Osmia excavata (Hymenoptera: Megachilidae) provides insights into low-temperature tolerance of Osmia pollinator.","authors":"Fang Ouyang, Honggang Guo, Wen Xie, Yingying Song, Zhuo Yan, Zhenqing Peng, Yongsheng Zhang, Ritao Qu, Huaigen Xin, Zheming Yuan, Zhishu Xiao, Xingyuan Men","doi":"10.1093/jee/toae257","DOIUrl":"https://doi.org/10.1093/jee/toae257","url":null,"abstract":"The solitary bee Osmia excavata (Hymenoptera: Megachilidae) is a key pollinator managed on a large scale. It has been widely used for commercial pollination of fruit trees, vegetables, and other crops with high efficiency in increasing the crop seeding rate, yield, and seed quality in Northern hemisphere. Here, a high-quality chromosome-level genome of O. excavata was generated using PacBio sequencing along with Hi-C technology. The genome size was 207.02 Mb, of which 90.25% of assembled sequences were anchored to 16 chromosomes with a contig N50 of 9,485 kb. Approximately 186.83 Mb, accounting for 27.93% of the genome, was identified as repeat sequences. The genome comprises 12,259 protein-coding genes, 96.24% of which were functionally annotated. Comparative genomics analysis suggested that the common ancestor of O. excavata and Osmia bicornis (Hymenoptera: Megachilidae) lived 8.54 million years ago. Furthermore, cytochrome P450 family might be involved in the responses of O. excavata to low-temperature stress. Taken together, the chromosome-level genome assembly of O. excavata provides in-depth knowledge and will be a helpful resource for the pollination biology research.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Agroecological importance of smooth brome in managing wheat stem sawfly (Hymenoptera: Cephidae) via associated braconid parasitoids. 平滑锦鸡儿通过相关的腕足动物寄生虫在管理小麦茎锯蝇（膜翅目：鳞翅目）方面的农业生态学重要性。

Journal of economic entomology Pub Date : 2024-10-28 DOI: 10.1093/jee/toae246

Jackson R Strand, Robert K D Peterson, Tracy M Sterling, David K Weaver

{"title":"Agroecological importance of smooth brome in managing wheat stem sawfly (Hymenoptera: Cephidae) via associated braconid parasitoids.","authors":"Jackson R Strand, Robert K D Peterson, Tracy M Sterling, David K Weaver","doi":"10.1093/jee/toae246","DOIUrl":"https://doi.org/10.1093/jee/toae246","url":null,"abstract":"Wheat stem sawfly (WSS), Cephus cinctus Norton (Hymenoptera: Cephidae), is a major pest of cereal crops throughout the Northern Great Plains of North America. Native parasitoids, Bracon cephi (Gahan) and B. lissogaster Muesebeck (Hymenoptera: Braconidae), play a key role in suppressing WSS populations and limiting associated damage. Smooth brome grass (Bromus inermis Leyss.) serves as a potential trap reservoir for WSS when grown in areas surrounding wheat (Triticum aestivum L.) fields in Montana. Its unique biology allows it to support high WSS infestation while promoting significant larval mortality throughout the growing season. Late-season WSS survivors can then serve as hosts for WSS parasitoids. Our study investigated smooth brome as a host refuge for WSS parasitoids. We measured WSS larval infestation and survival rate inside smooth brome grown within WSS-inclusion cages, finding a maximum infestation of 66.5% and an end-of-year WSS survival of 5.7%. In addition, we collected stems from sites in central and north-central Montana to measure the WSS infestation and parasitoid prevalence in wheat and adjacent smooth brome. WSS infestation was high in both Big Sandy (64.5% smooth brome, 65.7% adjacent wheat) and Moccasin, MT (50.6%, 38.6%). Year-end WSS larval mortality was 43.6% greater in smooth brome compared to adjacent wheat at both field sites, but both hosted similar numbers of WSS parasitoids. This research underscores the importance of smooth brome in providing a sustainable host refuge for WSS parasitoids and highlights its significant role in supporting the economics of wheat cultivation.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142523969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0