Journal of economic entomology最新文献_第2页

INFEST: an R web application to perform statistical analysis of insect feeding behavior data from EPG systems. INFEST：一个R web应用程序，用于对EPG系统中的昆虫摄食行为数据进行统计分析。

Journal of economic entomology Pub Date : 2025-09-25 DOI: 10.1093/jee/toaf232

Anderson Rodrigo da Silva, André Cirilo de Sousa Almeida, Flávio Gonçalves de Jesus, José Alexandre Freitas Barrigossi

{"title":"INFEST: an R web application to perform statistical analysis of insect feeding behavior data from EPG systems.","authors":"Anderson Rodrigo da Silva, André Cirilo de Sousa Almeida, Flávio Gonçalves de Jesus, José Alexandre Freitas Barrigossi","doi":"10.1093/jee/toaf232","DOIUrl":"https://doi.org/10.1093/jee/toaf232","url":null,"abstract":"Eletropenetrography (EPG) can help to unveil the feeding behavior of sucking insects feeding from a host of interest, including vegetal and animal tissues. It can be used to evaluate the effect of treatments such as insecticides. Based on a user's interpretation of electrical waveforms, a single EPG experiment can generate dozens of response variables associated with specific feeding activities. The variables consist, mostly, of duration and count of events. Some variables often present problematic data, such as an excess of zeros and overdispersion, which causes the classical statistical methods to be ineffective in discriminating treatments. In this article, we present INFEST-Insect Feeding Behavior Statistics, a user-friendly web application to process raw data files and to fit sophisticated statistical models to perform statistical analysis of EPG data. The program can import files from DC (direct current) and AC-DC (alternating current-direct current) EPG monitors' software and automatically calculates duration, count, and sequential variables. The statistical approach to compare experimental groups is based on Generalized Additive Models for Location, Shape, and Scale-GAMLSS, a very flexible class of parametric models that incorporates several probability distributions, including normal.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inside or out: insect foraging behavior drives trade-offs between berry number and berry size in highbush blueberry. 内部或外部：昆虫觅食行为驱动高丛蓝莓浆果数量和浆果大小之间的权衡。

Journal of economic entomology Pub Date : 2025-09-24 DOI: 10.1093/jee/toaf256

José N Mesquita-Neto, Bruna K Pinheiro-Costa

{"title":"Inside or out: insect foraging behavior drives trade-offs between berry number and berry size in highbush blueberry.","authors":"José N Mesquita-Neto, Bruna K Pinheiro-Costa","doi":"10.1093/jee/toaf256","DOIUrl":"https://doi.org/10.1093/jee/toaf256","url":null,"abstract":"Highbush blueberry (Vaccinium corymbosum L.) depends on insect pollinators, but frequently experiences pollination deficits, which limit crop yield and quality. Understanding the relationship between pollinator traits and pollination efficiency and their relative importance for pollen transfer, can provide valuable insights into how different foraging strategies influence pollination success. This study aimed to determine the influence of bee foraging behavior, specifically whether bees forage inside or outside the corolla, on pollination efficiency and fruit production in highbush blueberry. Fieldwork was carried out in 5 commercial blueberry orchards in southern Chile over 2 yr, where we recorded 893 flower visits from 16 species of bees. We classified pollinators into 2 behavioral groups: inside flower foragers (smaller-bodied native bees that enter the corolla) and outside flower foragers (larger-bodied bees that forage from the flower exterior). Using a single-visit pollination test and generalized linear mixed models, we quantified fruit set, fruit weight, and seed set by the 2 different foraging behaviors. Our results indicate that inside flower foragers significantly increased fruit set compared to unvisited flowers and outside flower foragers. However, outside flower foragers contributed more to fruit weight and seed set, highlighting a trade-off between pollination quantity and quality. This suggests that foraging behavior, rather than species identity alone, can serve as a predictive functional trait for pollination efficiency in blueberries. The behavior of foraging inside/outside the flower can serve as a functional trait to evaluate pollination efficiency, providing a new perspective beyond traditional species-level taxonomic identification.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sublethal and intergenerational effects of flonicamid on biological traits in the rose-grain aphid, Metopolophium dirhodum. 氟硝胺对玫瑰粒蚜生物学性状的亚致死效应和代际效应。

Journal of economic entomology Pub Date : 2025-09-24 DOI: 10.1093/jee/toaf234

Xinan Li, Chengze Song, Wen Zhang, Xiaoya Zhang, Fengfan Wang, Ruiyang Qin, Ganyu Zhu, Xun Zhu, Yunhui Zhang

{"title":"Sublethal and intergenerational effects of flonicamid on biological traits in the rose-grain aphid, Metopolophium dirhodum.","authors":"Xinan Li, Chengze Song, Wen Zhang, Xiaoya Zhang, Fengfan Wang, Ruiyang Qin, Ganyu Zhu, Xun Zhu, Yunhui Zhang","doi":"10.1093/jee/toaf234","DOIUrl":"https://doi.org/10.1093/jee/toaf234","url":null,"abstract":"The rose-grain aphid, Metopolophium dirhodum (Walker), has been identified as a predominant aphid species in wheat agroecosystems. Flonicamid, a novel pyridinamide insect growth regulator, has demonstrated high efficacy against various sap-sucking pests. We investigated the sublethal and intergenerational effects of flonicamid on M. dirhodum using life table analysis. We found that flonicamid had a certain degree of acute toxicity to M. dirhodum. Exposure to sublethal concentrations of flonicamid (LC15 and LC35) significantly reduced adult longevity, reproductive duration, and fecundity in the F0 generation of M. dirhodum. Moreover, these sublethal concentrations adversely affected the F1 generation, decreasing fecundity and altering key life table parameters, including adult longevity, total longevity, reproductive period, adult pre-reproductive period, nymph production per female, age-stage-specific survival rate (sxj), intrinsic rate of increase (r), net reproductive rate (R0), and finite rate of increase (λ). Population projections further demonstrated a significant decline in the total population size of M. dirhodum under sublethal flonicamid exposure compared to the control. These findings demonstrate that flonicamid exerts adverse sublethal and intergenerational effects on M. dirhodum, ultimately suppressing its population growth. The study outcomes provide valuable evidence for evaluating flonicamid's overall pesticidal potential and enhancing integrated pest management protocols for wheat aphid control.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Household and Structural InsectsIneffectiveness of over-the-counter bait and aerosol insecticide products against field-collected German cockroaches (Blattodea: Ectobiidae). 家用和结构昆虫：非处方毒饵和喷雾杀虫剂产品对野外采集的德国蜚蠊的有效性。

Journal of economic entomology Pub Date : 2025-09-24 DOI: 10.1093/jee/toaf248

Rattanan Chungsawat, Dong-Hwan Choe, Michael K Rust, Chow-Yang Lee

{"title":"Household and Structural InsectsIneffectiveness of over-the-counter bait and aerosol insecticide products against field-collected German cockroaches (Blattodea: Ectobiidae).","authors":"Rattanan Chungsawat, Dong-Hwan Choe, Michael K Rust, Chow-Yang Lee","doi":"10.1093/jee/toaf248","DOIUrl":"https://doi.org/10.1093/jee/toaf248","url":null,"abstract":"Over-the-counter (OTC) insecticide products are popular, easily available, and inexpensive do-it-yourself options for home residents. In this study, we investigated the performance of OTC insecticides consisting of bait and spray products against susceptible and field-collected strains of the German cockroach, Blattella germanica (L.) (Blattodea: Ectobiidae) using several test methods. In open arena bioassays, all OTC baits tested were ineffective on field-collected German cockroaches. The least effective OTC bait was unable to kill all test cockroaches of any strains at 14 d post-treatment. Direct spray experiments demonstrated that cockroaches treated with pyrethroid spray registered significantly shorter mean knockdown times when compared to that of the natural product. On the other hand, the natural product spray caused significantly shorter mean survival time in test insects than the pyrethroid spray (<1 h vs. >1 d). The residual efficacy of spray deposits was evaluated in the Ebeling choice box and revealed that all sprays have poor residual performance against field-collected German cockroach strains. Insecticide resistance and potential avoidance behavior may have contributed to the low performance index (PI) values of pyrethroid-based sprays, as cockroaches may have survived initial contact and subsequently avoided treated surfaces. Conversely, natural product spray deposits exhibited negative PI values for most of the study period due to spatial repellency. In general, the performance of OTC insecticide products was lower on field-collected German cockroach strains than on the susceptible strain. These products are likely ineffective at controlling the German cockroach infestations in homes.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nilaparvata lugens (Hemiptera: Delphacidae) feeding alters carbohydrate-redox homeostasis of rice plants grown under elevated CO2. 褐飞虱（半翅目：飞虱科）取食改变CO2升高条件下水稻碳水化合物氧化还原平衡。

Journal of economic entomology Pub Date : 2025-09-24 DOI: 10.1093/jee/toaf251

Rui-Chuan Duan, Jia-Cheng Huang, Xin-Ru Xiao, Fa-Jun Chen

{"title":"Nilaparvata lugens (Hemiptera: Delphacidae) feeding alters carbohydrate-redox homeostasis of rice plants grown under elevated CO2.","authors":"Rui-Chuan Duan, Jia-Cheng Huang, Xin-Ru Xiao, Fa-Jun Chen","doi":"10.1093/jee/toaf251","DOIUrl":"https://doi.org/10.1093/jee/toaf251","url":null,"abstract":"The rising atmospheric carbon dioxide (CO2) concentration due to global climate change may affect both plants and herbivorous insects. In this study, we explored the interactions between the feeding behavior of Nilaparvata lugens (Stål, 1854) (Hemiptera: Delphacidae) and rice plant responses-specifically photosynthesis, non-structural carbohydrates (NSCs), and oxidative stress under elevated CO2 (eCO2) conditions. The results showed that without N. lugens infestation, eCO2 enhanced rice plants' photosynthesis parameters and promoted NSCs accumulation and metabolism, while suppressing oxidative stress parameters compared to ambient CO2 (aCO2). However, under N. lugens infestation, eCO2-grown rice plants' photosynthesis parameters and NSCs levels declined, while oxidative stress parameters increased, ultimately surpassing those in aCO2-grown rice plants by 24 h. Electrical penetration graph (EPG) analysis revealed that N. lugens increased feeding activity on rice plants under eCO2 within the first 0 to 24 h, but reduced feeding between 24 and 36 h. Mantel test further verified that the feeding behavior of N. lugens was closely related to photosynthesis, NSCs and oxidative stress of rice plants under eCO2. This study demonstrates that eCO2 dynamically alters the interactions between rice plants and N. lugens by simultaneously modulating plant resource allocation and oxidative stress responses, thereby driving time-dependent shifts in the feeding behavior of N. lugens. These findings provide novel insights into how climate change may reshape trophic interactions in agroecosystems through physiological feedback mechanisms.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of sampling methods for figitid parasitoids of Drosophila suzukii (Diptera: Drosophilidae). 苏氏果蝇（双翅目：果蝇科）榕类寄生蜂取样方法的比较。

Journal of economic entomology Pub Date : 2025-09-24 DOI: 10.1093/jee/toaf207

Robert Czokajlo, Tobin Northfield, Louis Nottingham, Benjamin Diehl, Dylan Beal, Peter Smytheman, Elizabeth H Beers

{"title":"Comparison of sampling methods for figitid parasitoids of Drosophila suzukii (Diptera: Drosophilidae).","authors":"Robert Czokajlo, Tobin Northfield, Louis Nottingham, Benjamin Diehl, Dylan Beal, Peter Smytheman, Elizabeth H Beers","doi":"10.1093/jee/toaf207","DOIUrl":"https://doi.org/10.1093/jee/toaf207","url":null,"abstract":"Drosophila suzukii (Matsumura) is an invasive pest of berries and cherries in Washington State, and as a direct fruit feeder, has become a key pest in the affected crops. The recent interest in classical biological control using Asian parasitoids in the family Figitidae highlights the need for effective sampling methods. Whether adventively established or released by research labs, surveys and follow-up sampling for parasitoid detection is an important step in tracking their spread and ultimately, the success of release endeavors. In this study, we examined 5 proposed sampling approaches to determine their capture rates, relative effort, and ease of use. The study was conducted over 2 seasons in northwestern Washington State, where 2 Asian figitids, Ganaspis kimorum Buffington and Leptopilina japonica Novkovic & Kimura, were adventively established. Liquid-based D. suzukii traps using either wine-vinegar bait or an artificial lure captured ca. 10.6- to 256.6-fold more figitids than field collected fruit. However, liquid traps yielded mediocre specimens (for morphological identification or PCR analyses) and required considerable time to sort out the target species from by-catch. Yellow sticky cards with a synthetic lure had a similar capture rate as fruit sampling, but with poorer quality specimens. Fruit sampling yielded higher quality specimens and allowed for an association (including % parasitism) between host and parasitoid but was restricted to periods when ripe fruit was available. Deploying D. suzukii-infested fruit sentinels was labor intensive and captured only one adult figitid over 2 yr, suggesting that this was the least practical method.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insecticide and repellent tests on Washington leafhopper vectors of cherry X-disease. 樱桃x病华盛顿叶蝉病媒虫的杀虫剂和驱避试验。

Journal of economic entomology Pub Date : 2025-09-23 DOI: 10.1093/jee/toaf247

Adrian T Marshall, Katlyn A Catron, Robert J Orpet, R T Curtiss, Tobin D Northfield, Louis B Nottingham

{"title":"Insecticide and repellent tests on Washington leafhopper vectors of cherry X-disease.","authors":"Adrian T Marshall, Katlyn A Catron, Robert J Orpet, R T Curtiss, Tobin D Northfield, Louis B Nottingham","doi":"10.1093/jee/toaf247","DOIUrl":"https://doi.org/10.1093/jee/toaf247","url":null,"abstract":"A recent outbreak of X-disease, caused by the phytoplasma \"Candidatus Phytoplasma pruni,\" has severely harmed stone fruit production in the Pacific Northwest region of the United States. X-disease is spread by leafhopper vectors, primarily Colladonus montanus reductus in Washington and Euscelidius variegatus in Oregon. Growers rely on frequent insecticide applications and removal of infected trees, but these management recommendations originate from California when the last outbreak occurred in the 1980s. To update insecticide efficacy information for X-disease vectors, we evaluated 23 commercially available insecticidal products via direct spray, spray residues, systemic uptake by plants, or repellency assays. In direct spray bioassays, insecticides containing pyrethrins, pyrethroids, neonicotinoids, or organophosphates caused 100% mortality of C. m. reductus after 24 h. More selective chemistries including cyantraniliprole, flonicamid, and botanical essences also caused high mortality (>70%) of C. m. reductus (Van Duzee). For residues, neonicotinoids and pyrethroids caused the greatest mortality (≥70%) of C. m. reductus. Systemic uptake of neonicotinoids caused significantly higher mortality (51.4% to 100%) than controls (1.4% to 32.4%) of C. m. reductus. Direct sprays and 5 d residues of neonicotinoids caused high mortality (>70%) of E. variegatus. Cherry leaves treated with either kaolin clay or diatomaceous earth had 80% to 90% fewer C. m. reductus residing on them than the controls. Overall, pyrethroids and neonicotinoids were the most effective, but more selective chemistries and repellent clays showed potential and need to be validated in field conditions. Future work should integrate insecticides, repellents, and cultural tactics with phenology-based treatment timings to improve management sustainability.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of three figitid parasitoids of Drosophilidae in Washington State: a tale of 2 ecozones. 华盛顿州果蝇科3种拟蝇类的分布：以2个生态区为例。

Journal of economic entomology Pub Date : 2025-09-22 DOI: 10.1093/jee/toaf209

Robert Czokajlo, Chris Looney, Louis Nottingham, Benjamin Diehl, Paul K Abram, Tobin Northfield, Peter Smytheman, Elizabeth H Beers

{"title":"Distribution of three figitid parasitoids of Drosophilidae in Washington State: a tale of 2 ecozones.","authors":"Robert Czokajlo, Chris Looney, Louis Nottingham, Benjamin Diehl, Paul K Abram, Tobin Northfield, Peter Smytheman, Elizabeth H Beers","doi":"10.1093/jee/toaf209","DOIUrl":"https://doi.org/10.1093/jee/toaf209","url":null,"abstract":"The geographic distribution and relative abundance of 2 adventive Asian parasitoids (Ganaspis kimorum Buffington and Leptopilina japonica Novković & Kimura) (Hymenoptera: Figitidae) of spotted-wing drosophila, Drosophila suzukii Matsumura (Diptera: Drosophilidae) in Washington state were surveyed from 2020 to 2024 using bait or lure traps. G. kimorum and L. japonica were first detected in 2020 to 2021 in western Washington. However, their distribution in eastern Washington, which produces the majority Washington's vulnerable specialty crops, was of interest for establishing a baseline for future biological control efforts. A clear picture emerged regarding the current distribution of the 2 Asian species, and a cosmopolitan figitid, Leptopilina heterotoma (Thompson), which attacks other Drosophila spp. Adventive populations of G. kimorum were only found west of the Cascade Mountains; the few specimens recovered in eastern Washington were found near release sites. Conversely, L. heterotoma was rarely detected using jar traps in western Washington, but was the most abundant of the figitid species found in eastern Washington. L. japonica was common and abundant in both regions, but more so in western Washington. The unaided establishment of L. japonica in eastern Washington may presage its future impact as a biological control agent in this region.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145115746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioconversion of honey bee colony waste by small hive beetle (Coleoptera: Nitidulidae) larvae and the nutritional composition of larvae and frass. 小蜂房甲虫幼虫对蜂群废弃物的生物转化及幼虫和杂草的营养成分。

Journal of economic entomology Pub Date : 2025-09-22 DOI: 10.1093/jee/toaf220

Francisco Posada-Florez, Jayden C Steward, Emylia L Loaiza, Steven C Cook

{"title":"Bioconversion of honey bee colony waste by small hive beetle (Coleoptera: Nitidulidae) larvae and the nutritional composition of larvae and frass.","authors":"Francisco Posada-Florez, Jayden C Steward, Emylia L Loaiza, Steven C Cook","doi":"10.1093/jee/toaf220","DOIUrl":"https://doi.org/10.1093/jee/toaf220","url":null,"abstract":"A global demand for high protein animal feed and crop fertilizer continues to grow, and insects are an increasingly important alternative to conventional sources due to their reduced ecological and economic impacts. The small hive beetle (SHB), Aethina tumida (Murray) (Coleoptera: Nitidulidae), is a considerable cosmopolitan pest of Apis mellifera (Linnaeus) honey bees but may be a useful contributor to circular economies. SHB larvae are omnivorous, consuming pollen, honey, and honey bee brood. When matured, SHB larvae exit the hive to pupate in the soil, leaving their frass and other detritus behind. Here we present a method for rearing SHB larvae on excess stored pollen from honey bee colonies, calculate the bioconversion of the food source to larvae and frass, and report their nutritional content. Larvae showed a nearly 27% feed conversion efficiency, which is comparable or higher than that of other edible insect species. Larvae contained ∼25% of each protein and fatty acids and were low in carbohydrates (∼3%) and sugars (<0.4%). SHB larvae frass contained the highest nitrogen content compared to that of other edible insect taxa but was low in some other elements, such as iron. Given these data, the relatively innocuous larval diet, the \"self-harvesting\" behavior of the mature larvae, and the easily collected SHB frass, SHB larvae may be a valuable source of animal feed and biomanure. Frames of unused pollen may collect microbial contaminants, which may impose health issues for exposed honey bees. Removal of excess moldy pollen by SHB larvae may ameliorate these issues.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formula optimization of liquid medium for culturing the entomopathogenic nematode Steinernema carpocapsae All (Rhabditida: Steinernematidae). 昆虫病原线虫（拟虫目：拟虫科）培养液体培养基配方优化。

Journal of economic entomology Pub Date : 2025-09-22 DOI: 10.1093/jee/toaf190

Jingyin Tian, Guoyu Zhao, Xia Zhou, Feng Hu, Kangmeng Shao, Sajith Madhusanka Dassanayaka, Linhong Jin

{"title":"Formula optimization of liquid medium for culturing the entomopathogenic nematode Steinernema carpocapsae All (Rhabditida: Steinernematidae).","authors":"Jingyin Tian, Guoyu Zhao, Xia Zhou, Feng Hu, Kangmeng Shao, Sajith Madhusanka Dassanayaka, Linhong Jin","doi":"10.1093/jee/toaf190","DOIUrl":"https://doi.org/10.1093/jee/toaf190","url":null,"abstract":"The successful commercialization of entomopathogenic nematodes (EPNs) as a biopesticide product depends on industrial mass production of viable infective juveniles (IJs) at low cost. This study focused on enhancing the yield of the Steinernema carpocapsae All (Weiser) and reducing its production costs. An optimal basal medium was screened among several basal media, and several optimized components were included in the selected optimal basal medium. The optimal medium formulation was obtained by evaluating the yield, virulence, and survival rates of S. carpocapsae All at different times. The results showed that when isopropylstilbene was added to the basal medium together with insulin, the yield increased from 13.14 × 104 IJs/ml with the basal medium to 19.63 × 104 IJs/ml. At 30 d of low temperature storage, the survival rate increased from 31.42% (basal medium) to 56.83% (optimal medium), demonstrating a prolonged the shelf life. The virulence of S. carpocapsae All was measured before and after 30 d of storage on sponges. The virulence against Tenebrio molitor (Linnaeus) (Coleoptera: Tenebrionidae) and Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae) was 90 and 86.67% (before) and 93.33 and 94.44% (after), respectively, when insects were treated at 48 h, long-term cryogenic storage has no effect on the virulence. The yield with the individual optimization components added to the optimal basal medium was lower than when the components were combined, indicating that there may be a synergy between the 2 components. The results would provide practical technology for low-cost commercial production of S. carpocapsae All and promote field applications.","PeriodicalId":94077,"journal":{"name":"Journal of economic entomology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0