Ibrain Pub Date : 2023-08-24 DOI: 10.1002/ibra.12129

Anna-Maria V. Yerofeyeva, Sergey V. Pinchuk, Svetlana N. Rjabceva, Alla Y. Molchanova

{"title":"The role of cannabinoid CB1 receptors in the antinociceptive and reparative actions of mesenchymal stem cells in rats with peripheral neuropathic pain","authors":"Anna-Maria V. Yerofeyeva, Sergey V. Pinchuk, Svetlana N. Rjabceva, Alla Y. Molchanova","doi":"10.1002/ibra.12129","DOIUrl":"10.1002/ibra.12129","url":null,"abstract":"Mesenchymal stem cells (MSCs) can produce antinociceptive and reparative effects. Presumably, the MSCs-induced antinociception may be partly due to the involvement of the endocannabinoid system. The study aimed to evaluate the antinociceptive and reparative effects of adipose-derived MSCs (ADMSCs) upon pharmacological modulation of cannabinoid CB1 receptor in peripheral tissues or on ADMSCs' membranes in a rat model of peripheral neuropathy. ADMSCs were injected into the area of rat sciatic nerve injury (i) with no additional treatments, (ii) at the tissue CB1 receptor activation by endogenous agonist anandamide (AEA) or blockade with a selective AM251 antagonist; and (iii) preincubated with AEA or AM251. The evaluation of CB1 receptor activity involved analyzing nociceptive responses, gait parameters, and histology. Transplantation of ADMSCs upon activation of CB1 receptors, both on AMSCs' membranes or in the area of nerve injury, accelerated the analgesia and recovery of dynamic gait parameters, abolished static gait disturbances, and promoted the fastest nerve regeneration. Only blockade of CB1 receptors on ADMSCs shortened ADMSCs-induced analgesia and decreased the number of preserved nerve fibers. CB1 receptors on ADMSCs significantly contribute to their pain-relieving and tissue-repairing capabilities by stimulating the growth factors secretion and suppressing the release of pro-inflammatory cytokines. Peripheral CB1 receptors do not significantly influence ADMSC-induced antinociception.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"9 3","pages":"245-257"},"PeriodicalIF":0.0,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ibra.12129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41161256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CCA repair or ECA ligation—Which middle cerebral artery occlusion is better in the reperfusion mouse model? CCA修复还是ECA结扎在再灌注小鼠模型中哪种大脑中动脉闭塞更好？

Ibrain Pub Date : 2023-08-18 DOI: 10.1002/ibra.12128

Yue Hu, Zhen-Hong Yang, Feng Yan, Shuang-Feng Huang, Rong-Liang Wang, Zi-Ping Han, Jun-Fen Fan, Yang-Min Zheng, Ping Liu, Yu-Min Luo, Si-Jie Li

{"title":"CCA repair or ECA ligation—Which middle cerebral artery occlusion is better in the reperfusion mouse model?","authors":"Yue Hu, Zhen-Hong Yang, Feng Yan, Shuang-Feng Huang, Rong-Liang Wang, Zi-Ping Han, Jun-Fen Fan, Yang-Min Zheng, Ping Liu, Yu-Min Luo, Si-Jie Li","doi":"10.1002/ibra.12128","DOIUrl":"10.1002/ibra.12128","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 A reliable animal model is essential for ischemic stroke research. The implications of the external carotid artery (ECA) transection or common carotid artery (CCA) ligation have been described. Thus, a modified animal model, the CCA-repair model, has been established, and studies have shown that the CCA-repair model has potential advantages over the CCA-ligation model. However, whether the CCA-repair model is superior to the ECA-ligation model remains unclear. Sixty male C57BL/6 mice were randomly assigned to establish the CCA-repair (n = 34) or ECA-ligation (n = 26) models. Cerebral blood flow before middle cerebral artery occlusion (MCAO), immediately after MCAO and reperfusion were monitored and the operation duration, postoperative body weight, and food intake within 7 days, and the number of intraoperative and postoperative deaths within 7 days were recorded in the two models. Modified neurological severity scores and Bederson (0–5) scores were used to evaluate postoperative neurological function deficits on Days 1/3/5/7. 2,3,5-Triphenyltetrazolium chloride staining was used to quantify lesion volume on Day 7 after the operation. We found the establishment of the CCA-repair model required a longer total operation duration (p = 0.0175), especially the operation duration of reperfusion (p < 0.0001). However, there was no significant difference in body weight and food intake development, lesion volume and intragroup variability, neurological function deficits, mortality, and survival probability between the two groups. The CCA-repair model has no significant advantage over the ECA-ligation model. The ECA-ligation model is still a better choice for focal cerebral ischemia.\u0000 </section>\u0000 </div>","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"9 3","pages":"258-269"},"PeriodicalIF":0.0,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ibra.12128","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41160621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of Robo1 in the pentylenetetrazol-kindled epilepsy mouse model Robo1 在戊四唑诱发癫痫小鼠模型中的作用机制

Ibrain Pub Date : 2023-08-15 DOI: 10.1002/ibra.12127

Zheng Liu, Wei Huang, Man-Min Zhu, Zhong-Xiang Xu, Zu-Cai Xu, Chang-Yin Yu, Hao Huang

{"title":"Mechanism of Robo1 in the pentylenetetrazol-kindled epilepsy mouse model","authors":"Zheng Liu, Wei Huang, Man-Min Zhu, Zhong-Xiang Xu, Zu-Cai Xu, Chang-Yin Yu, Hao Huang","doi":"10.1002/ibra.12127","DOIUrl":"10.1002/ibra.12127","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 The neural network hypothesis is one of the important pathogenesis of drug-resistant epilepsy. Axons guide molecules through synaptic remodeling and brain tissue remodeling, which may result in the formation of abnormal neural networks. Therefore, axon guidance plays a crucial role in disease progression. However, although Robo1 is one of the important components of axon guidance, the role of Robo1 in epilepsy remains unclear. In this study, we aimed to explore the mechanism of Robo1 in epilepsy. Male adult C57BL/6 mice were intraperitoneally injected with pentylenetetrazol to establish an epilepsy model. Lentivirus (LV) was given via intracranial injection 2 weeks before pentylenetetrazol injection. Different expressions of Robo1 between the control group, LV-mediated Robo1 short hairpin RNA group, empty vector control LV group, and normal saline group were analyzed using Western blot, immunofluorescence staining, Golgi staining, and video monitoring. Robo1 was increased in the hippocampus in the pentylenetetrazol-induced epilepsy mouse model; lentiviral Robo1 knockdown prolonged the latency of seizure and reduced the seizure grade in mice and resulted in a decrease in dendritic spine density, while the number of mature dendritic spines was maintained. We speculate that Robo1 has been implicated in the development and progression of epilepsy through its effects on dendritic spine morphology and density. Epileptic mice with Robo1 knockdown virus intervention had lower seizure grade and longer latency. Follow-up findings suggest that Robo1 may modulate seizures by affecting dendritic spine density and morphology. Downregulation of Robo1 may negatively regulate epileptogenesis by decreasing the density of dendritic spines and maintaining a greater number of mature dendritic spines.\u0000 </section>\u0000 </div>","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"9 4","pages":"369-380"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ibra.12127","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81339762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress of postoperative cognitive dysfunction in cardiac surgery under cardiopulmonary bypass 心肺旁路下心脏手术术后认知功能障碍的研究进展

Ibrain Pub Date : 2023-08-14 DOI: 10.1002/ibra.12123

Yi-Ming Zhuang, Ji-Yang Xu, Kun Zheng, Hong Zhang

{"title":"Research progress of postoperative cognitive dysfunction in cardiac surgery under cardiopulmonary bypass","authors":"Yi-Ming Zhuang, Ji-Yang Xu, Kun Zheng, Hong Zhang","doi":"10.1002/ibra.12123","DOIUrl":"10.1002/ibra.12123","url":null,"abstract":"Cardiopulmonary bypass (CPB) is often used in cardiothoracic surgery because its nonphysiological state causes pathophysiological changes in the body, causing multiorgan and multitissue damage to varying degrees. Postoperative cognitive dysfunction (POCD) is a common central nervous system complication after cardiac surgery. The etiology and mechanism of POCD are not clear. Neuroinflammation, brain mitochondrial dysfunction, cerebral embolism, ischemia, hypoxia, and other factors are related to the pathogenesis of POCD. There is a close relationship between CPB and POCD, as CPB can cause inflammation, hypoxia and reperfusion injury, and microemboli formation, all of which can trigger POCD. POCD increases medical costs, seriously affects patients' quality of life, and increases mortality. Currently, there is a lack of effective treatment methods for POCD. Commonly used methods include preoperative health management, reducing inflammation response during surgery, preventing microemboli formation, and implementing individualized rehabilitation programs after surgery. Strengthening preventive measures can minimize the occurrence of POCD and its adverse effects.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"10 3","pages":"290-304"},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ibra.12123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75458583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Double gene mutations of LRSAM1 and REEP1 and a new REEP1 mutation site found in a patient with amyotrophic lateral sclerosis with subjective paresthesia: A case report LRSAM1和REEP1双基因突变和一个新的REEP1突变位点在肌萎缩侧索硬化症伴有主观感觉异常的患者中发现:1例报告

Ibrain Pub Date : 2023-08-14 DOI: 10.1002/ibra.12125

Jiahui Qin, Zun‐Lin Zhou, Yuankun Zhou, Ya Chen, Xiao‐Yan Yang, Hai‐Qing Zhang, Zucai Xu

{"title":"Double gene mutations of LRSAM1 and REEP1 and a new REEP1 mutation site found in a patient with amyotrophic lateral sclerosis with subjective paresthesia: A case report","authors":"Jiahui Qin, Zun‐Lin Zhou, Yuankun Zhou, Ya Chen, Xiao‐Yan Yang, Hai‐Qing Zhang, Zucai Xu","doi":"10.1002/ibra.12125","DOIUrl":"https://doi.org/10.1002/ibra.12125","url":null,"abstract":"Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by selective degeneration of upper and lower motor neurons. Although dyskinesia is the most prominent clinical manifestation of ALS, with an in‐depth understanding of disease pathogenesis and clinical detection, more and more ALS patients are found to have nonmotor symptoms, such as sensory impairment. Genetic testing technology has developed rapidly in recent years. New genes have been proven to be involved in the pathogenesis of ALS. However, according to the existing research evidence, no literature has reported that patients with ALS have leucine‐rich repeats and sterility α mutations in motif 1 (LRSAM1) and receptor expression accessory protein 1 (REEP1). The mutation sites of REEP1 gene have not been reported, and the simultaneous mutations of two genes have not been reported. In the largest human gene mutation frequency database gnomad, the mutation sites of two genes are currently defined as new heterozygous variants with unclear clinical significance. Therefore, this article reports the clinical data of this case to further deepen the clinicians' understanding of the disease, and may provide evidence for further study of the new genotype–phenotype of LRSAM1 and REEP1.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"2016 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86594547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gold and silver nanoparticles in Alzheimer's and Parkinson's diagnostics and treatments 阿尔茨海默氏症和帕金森氏症诊断和治疗中的金和银纳米颗粒。

Ibrain Pub Date : 2023-08-14 DOI: 10.1002/ibra.12126

Edoardo Scarpa, Mariafrancesca Cascione, Anna Griego, Paolo Pellegrino, Giorgia Moschetti, Valeria De Matteis

{"title":"Gold and silver nanoparticles in Alzheimer's and Parkinson's diagnostics and treatments","authors":"Edoardo Scarpa, Mariafrancesca Cascione, Anna Griego, Paolo Pellegrino, Giorgia Moschetti, Valeria De Matteis","doi":"10.1002/ibra.12126","DOIUrl":"10.1002/ibra.12126","url":null,"abstract":"Neurodegenerative diseases (NDs) impose substantial medical and public health burdens on people worldwide and represent one of the major threats to human health. The prevalence of these age-dependent disorders is dramatically increasing over time, a process intrinsically related to a constantly rising percentage of the elderly population in recent years. Among all the NDs, Alzheimer's and Parkinson's are considered the most debilitating as they cause memory and cognitive loss, as well as severely affecting basic physiological conditions such as the ability to move, speak, and breathe. There is an extreme need for new and more effective therapies to counteract these devastating diseases, as the available treatments are only able to slow down the pathogenic process without really stopping or resolving it. This review aims to elucidate the current nanotechnology-based tools representing a future hope for NDs treatment. Noble metal nano-systems, that is, gold and silver nanoparticles (NPs), have indeed unique physicochemical characteristics enabling them to deliver any pharmacological treatment in a more effective way within the central nervous system. This can potentially make NPs a new hope for reversing the actual therapeutic strategy based on slowing down an irreversible process into a more effective and permanent treatment.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"9 3","pages":"298-315"},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ibra.12126","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41180784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA methylation: The epigenetic mechanism of Alzheimer's disease DNA 甲基化：阿尔茨海默病的表观遗传学机制

Ibrain Pub Date : 2023-08-10 DOI: 10.1002/ibra.12121

Hao-Yue Qin, Jiao-Yan Liu, Chang-Le Fang, Yan-Ping Deng, Ying Zhang

引用次数: 0

The interaction between instrumental activities of daily living and dual sensory function on cognition among the elderly in China: A cross-sectional survey 中国老年人日常生活工具性活动与认知双重感觉功能的互动：一项横断面调查。

Ibrain Pub Date : 2023-08-09 DOI: 10.1002/ibra.12124

Heting Liang, Zhixia Jiang, Xiaoling Yang, Shuang Li, Xiaoling Zhao, Yongya Dai, Siqin Liu, Yumeng Zhang, Xiaoli Yuan

{"title":"The interaction between instrumental activities of daily living and dual sensory function on cognition among the elderly in China: A cross-sectional survey","authors":"Heting Liang, Zhixia Jiang, Xiaoling Yang, Shuang Li, Xiaoling Zhao, Yongya Dai, Siqin Liu, Yumeng Zhang, Xiaoli Yuan","doi":"10.1002/ibra.12124","DOIUrl":"10.1002/ibra.12124","url":null,"abstract":"To explore the interaction of instrumental activities of daily living (IADLs) and dual sensory function on cognition in the elderly. A cross-sectional survey was conducted in six general hospitals in China, from October 2022 to December 2022. Data collection included general information, IADLs scale, self-reported sensory function questionnaire, and mini-mental state examination (MMSE). Binary logistic regression was used to examine the association between factors and cognition. The interactive effect was evaluated by synergy index (S), relative excess risk due to interaction (RERI), and attributable proportion due to interaction (AP). The odds ratio (OR) of IADLs decline in cognition is 4.412 (95% confidence interval [CI]: 3.633–5.358, p < 0.001); the OR of dual sensory difficulty on cognition is 2.502 (95% CI: 1.272–4.921, p = 0.008). The OR of interaction between IADLs decline and dual sensory difficulty on cognition is 13.737 (95% CI: 9.726–19.400, p < 0.001). RERI (95% CI) = 7.823 (3.230–12.417), AP (95% CI) = 0.570 (0.392–0.747), S (95% CI) = 2.593 (1.616–4.160). IADLs decline and dual sensory difficulty are associated with cognitive decline. IADLs decline and dual sensory difficulty have interaction with cognitive decline; the interaction is greater than the sum effect of those two on cognitive decline independently. Sensory and IADLs assessment can be used as early screening items for cognition among the elderly. In addition, protecting sensory function and maintaining IADLs in the elderly can help protect their cognition.","PeriodicalId":94030,"journal":{"name":"Ibrain","volume":"9 3","pages":"281-289"},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ibra.12124","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41163400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Routes and methods of neural stem cells injection in cerebral ischemia 脑缺血时神经干细胞注射的途径和方法。

Ibrain Pub Date : 2023-08-06 DOI: 10.1002/ibra.12122

Xing-Yu Yang, Xiao Zhang, Jun-Feng Cao, Mei Wu, Sheng-Yan Chen, Li Chen

引用次数: 0

Study on the efficacy of compound porcine cerebroside and ganglioside injection in patients with ischemic stroke: A randomized, single‐center, open‐label, prospective study 复方猪脑苷和神经节苷脂注射液对缺血性脑卒中患者的疗效研究:一项随机、单中心、开放标签、前瞻性研究

Ibrain Pub Date : 2023-07-15 DOI: 10.1002/ibra.12120

Ya Chen, Xia Zhang, Hai‐Qing Zhang, Zhong Luo, Xueyan Zhou, Tao Liang, Fei Yang, Jun Zhang, Zucai Xu

引用次数: 0

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