European journal of microbiology & immunology最新文献_第4页

Association between ABO and Rh blood groups and Toxoplasma gondii infection: A cross sectional study. ABO 和 Rh 血型与弓形虫感染的关系：一项横断面研究。

European journal of microbiology & immunology Pub Date : 2024-01-12 Print Date: 2024-02-23 DOI: 10.1556/1886.2023.00052

Alma Rosa Pérez-Álamos, Marisela Aguilar-Durán, Sergio Estrada Martínez, Agar Ramos-Nevárez, Carlos Alberto Guido-Arreola, Antonio Sifuentes-Álvarez, Sandra Margarita Cerrillo-Soto, Raúl Graciano Ibarra, Cosme Alvarado-Esquivel

{"title":"Association between ABO and Rh blood groups and Toxoplasma gondii infection: A cross sectional study.","authors":"Alma Rosa Pérez-Álamos, Marisela Aguilar-Durán, Sergio Estrada Martínez, Agar Ramos-Nevárez, Carlos Alberto Guido-Arreola, Antonio Sifuentes-Álvarez, Sandra Margarita Cerrillo-Soto, Raúl Graciano Ibarra, Cosme Alvarado-Esquivel","doi":"10.1556/1886.2023.00052","DOIUrl":"10.1556/1886.2023.00052","url":null,"abstract":"We aimed to determine the association between the seropositivity to Toxoplasma gondii and the ABO and Rh blood groups in 2,053 people. ABO and Rhesus blood groups and anti-T. gondii IgG and IgM antibodies were determined using commercially available assays. Of the 2,053 people studied, 171 (8.3%) were positive for anti-T. gondii IgG antibodies. Sixty-five (38.0%) and 36 (21.1%) of these 171 individuals had high anti-T. gondii IgG antibody levels (≥150 IU mL-1) and anti-T. gondii IgM antibodies, respectively. We found the following prevalences of T. gondii infection among the ABO groups: 8.5% in group A, 4.3% in group B, 4.7% in group AB, and 8.9% in group O (P = 0.19). The prevalences of T. gondii infection among Rh groups were: 8.4% in the Rh-positive group and 7.1% in the Rh-negative group (P = 0.58). Logistic regression analysis showed that the frequencies of ABO and Rh blood groups were similar (P > 0.05) among people with positive and negative serology for anti-T. gondii IgG antibodies, with high (≥150 IU mL-1) and lower (<150 IU mL-1) levels of anti-T. gondii IgG antibodies, and with positive and negative serology for anti-T. gondii IgM antibodies. Results does not support an association between T. gondii infection and ABO and Rh blood groups.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10895359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139428091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prophylactic oral application of resveratrol to alleviate acute campylobacteriosis in human gut microbiota associated IL-10-/- mice. 预防性口服白藜芦醇可缓解与人类肠道微生物群相关的 IL-10-/- 小鼠的急性弯曲杆菌病。

European journal of microbiology & immunology Pub Date : 2023-12-15 Print Date: 2023-12-21 DOI: 10.1556/1886.2023.00042

Markus M Heimesaat, Niklas Schabbel, Luis Q Langfeld, Nizar W Shayya, Soraya Mousavi, Stefan Bereswill

{"title":"Prophylactic oral application of resveratrol to alleviate acute campylobacteriosis in human gut microbiota associated IL-10-/- mice.","authors":"Markus M Heimesaat, Niklas Schabbel, Luis Q Langfeld, Nizar W Shayya, Soraya Mousavi, Stefan Bereswill","doi":"10.1556/1886.2023.00042","DOIUrl":"10.1556/1886.2023.00042","url":null,"abstract":"Human infections with the food-borne zoonotic enteropathogen Campylobacter jejuni are increasing globally. Since multi-drug resistant bacterial strains are further on the rise, antibiotic-independent measures are needed to fight campylobacteriosis. Given its anti-microbial and anti-inflammatory properties the polyphenolic compound resveratrol constitutes such a promising candidate molecule. In our present placebo-controlled intervention trial, synthetic resveratrol was applied perorally to human gut microbiota-associated (hma) IL-10-/- mice starting a week before oral C. jejuni infection. Our analyses revealed that the resveratrol prophylaxis did not interfere with the establishment of C. jejuni within the murine gastrointestinal tract on day 6 post-infection, but alleviated clinical signs of campylobacteriosis and resulted in less distinct colonic epithelial apoptosis. Furthermore, oral resveratrol dampened C. jejuni-induced colonic T and B cell responses as well as intestinal secretion of pro-inflammatory mediators including nitric oxide, IL-6, TNF-α, and IFN-γ to basal levels. Moreover, resveratrol application was not accompanied by significant shifts in the colonic commensal microbiota composition during campylobacteriosis in hma IL-10-/- mice. In conclusion, our placebo-controlled intervention study provides evidence that prophylactic oral application of resveratrol constitutes a promising strategy to alleviate acute campylobacteriosis and in consequence, to reduce the risk for post-infectious autoimmune sequelae.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10755668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138813548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Campylobacter in Africa - A specific viewpoint. 非洲的弯曲杆菌--一种特殊观点。

European journal of microbiology & immunology Pub Date : 2023-12-05 Print Date: 2023-12-21 DOI: 10.1556/1886.2023.00043

Ellis Kobina Paintsil, Wycliffe O Masanta, Annika Dreyer, Leonid Ushanov, Stella I Smith, Hagen Frickmann, Andreas E Zautner

{"title":"Campylobacter in Africa - A specific viewpoint.","authors":"Ellis Kobina Paintsil, Wycliffe O Masanta, Annika Dreyer, Leonid Ushanov, Stella I Smith, Hagen Frickmann, Andreas E Zautner","doi":"10.1556/1886.2023.00043","DOIUrl":"10.1556/1886.2023.00043","url":null,"abstract":"Campylobacter infections and campylobacteriosis-associated post-infectious sequelae are a significant global health burden that needs to be addressed from a specific African perspective. We conducted a comprehensive literature search on NCBI PubMed to compile a comprehensive narrative review article on Campylobacter infections in Africa, focusing on key aspects in human and veterinary medicine as well as food hygiene. We specifically focused on the epidemiology of enteropathogenic Campylobacter spp. in sub-Saharan and North Africa considering antimicrobial susceptibility. The most significant sequela resulting from molecular mimicry to Campylobacter surface structures is the Guillain-Barré syndrome, which was mainly examined in the context of limited studies conducted in African populations. A dedicated subsection is allocated to the limited research on the veterinary medically important species Campylobacter fetus. There are significant differences in the composition of the gut microbiome, especially in rural areas, which affect the colonization with Campylobacter spp. and the manifestation of campylobacteriosis. There may be a problem of overdiagnosis due to asymptomatic colonization, particularly in the detection of Campylobacter using molecular biological techniques. To reduce the colonization and infection rate of Campylobacter, we propose implementing several control measures and urge further research to improve the current understanding of the peculiarities of campylobacteriosis in Africa.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10755667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138489399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Actinomyces spp. and related organisms in the development of medication-related osteonecrosis of the jaw (MRONJ): Clinical evidence based on a case series. 放线菌及其相关生物在药物相关性颌骨骨坏死(MRONJ)发展中的作用:基于病例系列的临床证据。

European journal of microbiology & immunology Pub Date : 2023-12-01 Print Date: 2023-12-21 DOI: 10.1556/1886.2023.00041

Zsanett Kövér, Ágnes Bán, Márió Gajdács, Beáta Polgár, Edit Urbán

{"title":"Role of Actinomyces spp. and related organisms in the development of medication-related osteonecrosis of the jaw (MRONJ): Clinical evidence based on a case series.","authors":"Zsanett Kövér, Ágnes Bán, Márió Gajdács, Beáta Polgár, Edit Urbán","doi":"10.1556/1886.2023.00041","DOIUrl":"10.1556/1886.2023.00041","url":null,"abstract":"Medication-related osteonecrosis of the jaw (MRONJ) is an increasingly common consequence of antiresorptive treatment, which often leads to the development of necrotic exposed bone surfaces with inflammatory processes affecting the jawbone. Although the development of MRONJ is often associated with the inflammatory response or infections caused by the colonizing members of the oral microbiota, the exact pathogenesis of MRONJ is still not fully understood. In the present paper, we aimed to provide additional, microbiological culture-supported evidence, supporting the \"infection hypothesis\" that Actinomyces spp. and related organisms may play an important pathogenic role in the development of MRONJ and the resulting bone necrosis. In our case series, all patients presented with similar underlying conditions and anamnestic data, and have received antiresorptive medications (bisphosphonates or a RANK ligand (RANKL) inhibitor) to prevent the occurrence or progression of bone metastases, secondary to prostate cancer. Nevertheless, a few years into antiresorptive drug therapy, varying stages of MRONJ was identified in the mentioned patients. In all three cases, quantitative microbiological culture of the necrotic bone samples yielded a complex microbiota, dominated by Actinomyces and Schaalia spp. with high colony counts. Additionally, our followed-up case series document the treatment of these patients with a combination of surgical intervention and long-term antibiotic therapy, where favourable clinical responses were seen is all cases. If the \"infection hypothesis\" is valid, it may have significant consequences in the preventative and therapeutic strategies associated with this disease.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10755666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138465153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral application of carvacrol, butyrate, ellagic acid, and 2'-fucosyl-lactose to mice suffering from acute campylobacteriosis - Results from a preclinical placebo-controlled intervention study. 口服香芹酚、丁酸、鞣花酸和2′-焦酰基乳糖治疗急性弯曲菌病小鼠——临床前安慰剂对照干预研究结果

European journal of microbiology & immunology Pub Date : 2023-11-21 Print Date: 2023-11-23 DOI: 10.1556/1886.2023.00037

Soraya Mousavi, Lia V Busmann, Rasmus Bandick, Nizar W Shayya, Stefan Bereswill, Markus M Heimesaat

{"title":"Oral application of carvacrol, butyrate, ellagic acid, and 2'-fucosyl-lactose to mice suffering from acute campylobacteriosis - Results from a preclinical placebo-controlled intervention study.","authors":"Soraya Mousavi, Lia V Busmann, Rasmus Bandick, Nizar W Shayya, Stefan Bereswill, Markus M Heimesaat","doi":"10.1556/1886.2023.00037","DOIUrl":"10.1556/1886.2023.00037","url":null,"abstract":"Background: Acute campylobacteriosis caused by oral infections with the enteropathogen Campylobacter jejuni represent serious threats to global human health. Since novel treatment options with safe and antibiotics-independent compounds would be highly appreciable, we here investigated the anti-bacterial and disease-alleviating effects of carvacrol, butyrate, ellagic acid, and 2'-fucosyl-lactose in acute murine campylobacteriosis. To address this, secondary abiotic IL-10-/- mice were perorally infected with C. jejuni and treated with either compound alone or all four in combination via the drinking water starting two days post-infection.Results: On day 6, the duodenal pathogen loads were lower in mice of the combination versus the vehicle treatment cohort. Importantly, mice treated with carvacrol and the combination presented with less distinct diarrheal symptoms, colonic histopathology, epithelial cell apoptosis, and immune cell responses when compared to vehicle counterparts on day 6 post-infection. Furthermore, the combination treatment did not only diminish colonic IFN-γ, TNF-α, and IL-6 secretion in C. jejuni infected mice, but also dampened extra-intestinal and even systemic pro-inflammatory cytokine concentrations to basal levels as measured in liver, kidneys, lungs, and serum samples.Conclusions: Our preclinical placebo-controlled intervention trial provides evidence that the combined oral application of carvacrol, butyrate, ellagic acid, and 2'-fucosyl-lactose alleviates acute campylobacteriosis in the vertebrate host.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138178289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxoplasma gondii infection of the central nervous system and suicide: A case-control study of decedents. 刚地弓形虫感染中枢神经系统与自杀:死者病例对照研究。

European journal of microbiology & immunology Pub Date : 2023-11-20 Print Date: 2023-11-23 DOI: 10.1556/1886.2023.00033

Laura Alejandra Mendoza-Larios, Fernando García-Dolores, Luis Francisco Sánchez-Anguiano, Elizabeth Irasema Antuna-Salcido, Jesús Hernández-Tinoco, Adriana Rocha-Salais, Marcela Araceli Segoviano-Mendoza, Antonio Sifuentes-Álvarez, Cosme Alvarado-Esquivel

引用次数: 0

Global transcriptomic analysis of breast cancer and normal mammary epithelial cells infected with Borrelia burgdorferi. 伯氏疏螺旋体感染的癌症和正常乳腺上皮细胞的全转录组学分析。

European journal of microbiology & immunology Pub Date : 2023-10-19 Print Date: 2023-11-23 DOI: 10.1556/1886.2023.00031

Vishwa A Khatri, Sambuddha Paul, Niraj Jatin Patel, Sahaja Thippani, Janhavi Y Sawant, Katie L Durkee, Cassandra L Murphy, Geneve Ortiz Aleman, Justine A Valentino, Jasmine Jathan, Anthony Melillo, Eva Sapi

{"title":"Global transcriptomic analysis of breast cancer and normal mammary epithelial cells infected with Borrelia burgdorferi.","authors":"Vishwa A Khatri, Sambuddha Paul, Niraj Jatin Patel, Sahaja Thippani, Janhavi Y Sawant, Katie L Durkee, Cassandra L Murphy, Geneve Ortiz Aleman, Justine A Valentino, Jasmine Jathan, Anthony Melillo, Eva Sapi","doi":"10.1556/1886.2023.00031","DOIUrl":"10.1556/1886.2023.00031","url":null,"abstract":"The bacterial spirochete Borrelia burgdorferi, the causative agent of Lyme Disease, can disseminate and colonize various tissues and organs, orchestrating severe clinical symptoms including arthritis, carditis, and neuroborreliosis. Previous research has demonstrated that breast cancer tissues could provide an ideal habitat for diverse populations of bacteria, including B. burgdorferi, which is associated with a poor prognosis. Recently, we demonstrated that infection with B. burgdorferi enhances the invasion and migration of triple-negative MDA-MB-231 cells which represent a type of breast tumor with more aggressive cancer traits. In this study, we hypothesized that infection by B. burgdorferi affects the expression of cancer-associated genes to effectuate breast cancer phenotypes. We applied the high-throughput technique of RNA-sequencing on B. burgdorferi-infected MDA-MB-231 breast cancer and normal-like MCF10A cells to determine the most differentially expressed genes (DEG) upon infection. Overall, 142 DEGs were identified between uninfected and infected samples in MDA-MB-231 while 95 DEGs were found in MCF10A cells. A major trend of the upregulation of C-X-C and C-C motif chemokine family members as well as genes and pathways was associated with infection, inflammation, and cancer. These genes could serve as potential biomarkers for pathogen-related tumorigenesis and cancer progression which could lead to new therapeutic opportunities.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49686566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effect of theaflavin-3,3'-digallate can involve its binding to the "stem" domain of α-hemolysin of Staphylococcus aureus. 茶黄素-3,3'-二没食子酸盐的抑制作用可能涉及其与金黄色葡萄球菌α-溶血素的“茎”结构域的结合。

European journal of microbiology & immunology Pub Date : 2023-10-18 Print Date: 2023-11-23 DOI: 10.1556/1886.2023.00032

Anna Goc, Waldemar Sumera, Matthias Rath, Aleksandra Niedzwiecki

{"title":"Inhibitory effect of theaflavin-3,3'-digallate can involve its binding to the \"stem\" domain of α-hemolysin of Staphylococcus aureus.","authors":"Anna Goc, Waldemar Sumera, Matthias Rath, Aleksandra Niedzwiecki","doi":"10.1556/1886.2023.00032","DOIUrl":"10.1556/1886.2023.00032","url":null,"abstract":"Infections caused by Staphylococcus aureus are currently a worldwide threat affecting millions of individuals. The pathogenicity of S. aureus is associated with numerous virulence factors, including cell surface proteins, polysaccharides, and secreted toxins. The pore-forming α-hemolysin, known as α-toxin, is produced by nearly all virulent strains of S. aureus and is implicated in several diseases including skin and soft tissue infections, atopic dermatitis, and pneumonia. There are currently no vaccines available for the prevention of S. aureus infections and the efficacy of available antibiotics has been fading. In this study we examined the mode of antihemolytic activity of theaflavin-3,3'-digallate against α-hemolysin of methicillin-resistant S. aureus by molecular docking using AutoDock Vina as the molecular docking tool. The theaflavin-3,3'-digallate docked the molecular sequence of the Hla (PDB ID:7ahl). The scores of the top 10 binding modes obtained were between -9.0 and -8.5 kcal mol-1, and the best binding mode was -9.0 kcal mol-1. Direct binding sites of theaflavin-3,3'-digallate to the \"stem\" domain of Hla were revealed which primarily targeted of the residues Met113, Thr117, Asn139. The disclosure of this potential binding mode warrants further clinical evaluation of theaflavin-3,3'-digallate as an anti-hemolytic compound in order to practically validate our results.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10668921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49686567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Very low prevalence of Mansonella perstans-specific cell-free DNA in serum samples of Ghanaian HIV patients. 加纳HIV患者血清样本中的Mansonella perstans特异性无细胞DNA的患病率非常低。

European journal of microbiology & immunology Pub Date : 2023-09-26 DOI: 10.1556/1886.2023.00028

Kirsten Alexandra Eberhardt, Luzia Veletzky, Felix Weinreich, Richard Odame Phillips, Fred Stephen Sarfo, Torsten Feldt, Albert Dompreh, Shadrack Osei Asibey, Richard Boateng, Hagen Frickmann

{"title":"Very low prevalence of Mansonella perstans-specific cell-free DNA in serum samples of Ghanaian HIV patients.","authors":"Kirsten Alexandra Eberhardt, Luzia Veletzky, Felix Weinreich, Richard Odame Phillips, Fred Stephen Sarfo, Torsten Feldt, Albert Dompreh, Shadrack Osei Asibey, Richard Boateng, Hagen Frickmann","doi":"10.1556/1886.2023.00028","DOIUrl":"10.1556/1886.2023.00028","url":null,"abstract":"Background: Mansonellosis is a widely neglected helminth disease which is predominantly observed in tropical regions. This study was conducted to assess potential associations of the prevalence of circulating Mansonella perstans-specific cell-free DNA in human serum and HIV infection in Ghanaian individuals.Methods: For this purpose, serum samples obtained from Ghanaian HIV-patients (n = 989) and non-HIV-infected Ghanaian control individuals (n = 91) were subjected to real-time PCR targeting the ITS-(internal transcribed spacer-)2 sequence of M. perstans and Mansonella sp. Deux.Results: Mansonella-specific cell-free DNA was detected in serum samples of only 2 HIV-positive and 0 HIV-negative individuals, making any reliable conclusions on potential associations between HIV and mansonellosis in tropical Ghana unfeasible.Conclusions: Future epidemiological studies on hypothetical associations between mansonellosis and HIV infections should focus more specifically on high-endemicity settings for both Mansonella spp.-infections and HIV-infections, include higher case numbers and be based on real-time PCR from whole blood rather than from serum, in which only circulating parasite DNA but no more cell-bound parasite DNA can be detected. However, the study did not show associations of HIV infections in Ghanaian individuals with Mansonella worm loads high enough to detect cell-free Mansonella DNA in serum by PCR.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/21/59/eujmi-13-057.PMC10578140.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41171988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and optimization of an improved qPCR assay for the detection of Histoplasma capsulatum. 用于检测荚膜组织浆的改良qPCR检测方法的设计和优化。

European journal of microbiology & immunology Pub Date : 2023-09-21 DOI: 10.1556/1886.2023.00026

Bianca Kelly Neves Izidro da Silva, Ana Cláudia Alves Cortez, Luciana Aires Oliveira, Juan Diego Ribeiro de Almeida, Érica Simplício de Souza, Katia Santana Cruz, Ani Beatriz Jackisch Matsuura, Mauricio Morishi Ogusku, Karolina Jeaneth Solórzano Chavarría, Djane Baía-da-Silva, Quique Bassat, Marcus Vinícius Guimarães Lacerda, Hagen Frickmann, João Vicente Braga de Souza

{"title":"Design and optimization of an improved qPCR assay for the detection of Histoplasma capsulatum.","authors":"Bianca Kelly Neves Izidro da Silva, Ana Cláudia Alves Cortez, Luciana Aires Oliveira, Juan Diego Ribeiro de Almeida, Érica Simplício de Souza, Katia Santana Cruz, Ani Beatriz Jackisch Matsuura, Mauricio Morishi Ogusku, Karolina Jeaneth Solórzano Chavarría, Djane Baía-da-Silva, Quique Bassat, Marcus Vinícius Guimarães Lacerda, Hagen Frickmann, João Vicente Braga de Souza","doi":"10.1556/1886.2023.00026","DOIUrl":"10.1556/1886.2023.00026","url":null,"abstract":"Background: This study aimed at improving a real-time polymerase-chain-reaction (qPCR) assay for the detection of Histoplasma capsulatum, a fungal pathogen that can cause severe respiratory infections in humans, in clinical and soil samples.Methods: Primer and probes were in-silico designed, in-silico and in-vitro evaluated including clinical biopsy materials and finally subjected to a real-world application with collected soil samples.Results: Applying the qPCR assay with liver and lung biopsies from 71 patients each, including 59 patients infected with human immunodeficiency virus (HIV), as well as with Sabouraud (SAB) agar culture as the diagnostic reference standard, diagnostic accuracy of the qPCR assay of 100% (5/5) sensitivity and 96% (63/66) specificity for liver samples and 100% (4/4) sensitivity and 94% (63/67) specificity for the lung samples was recorded. When applying the assay with soil samples from caves near of Presidente Figueiredo city, Amazonas, Brazil, one sample from the Maroaga cave was confirmed as positive.Conclusions: The improved qPCR assessed in this study was successful in detecting H. capsulatum with high efficiency and accuracy in in-vitro evaluation, including the identification of the target pathogen in both clinical and environmental samples.","PeriodicalId":93998,"journal":{"name":"European journal of microbiology & immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/58/5e/eujmi-13-037.PMC10578137.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41180057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0