Canadian journal of veterinary research = Revue canadienne de recherche veterinaire最新文献_第5页

Effect of dietary iron supplementation on the equine fecal microbiome. 日粮补铁对马粪便微生物组的影响。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2023-04-01

Julia Assis Arantes, Alexandre Secorun Borges, Luiza Stachewski Zakia, Michael Gordon Surette, Jeffrey Scott Weese, Marcio Carvalho Costa, Luis Guillermo Arroyo

{"title":"Effect of dietary iron supplementation on the equine fecal microbiome.","authors":"Julia Assis Arantes, Alexandre Secorun Borges, Luiza Stachewski Zakia, Michael Gordon Surette, Jeffrey Scott Weese, Marcio Carvalho Costa, Luis Guillermo Arroyo","doi":"","DOIUrl":"","url":null,"abstract":"Iron is an essential element for all living organisms, including bacteria, as several virulence factors and replication components are influenced by iron concentration. The objective of this study was to determine whether the composition and diversity of the fecal microbiota of adult horses are affected by supplemental dietary iron. Ten clinically healthy horses were randomly divided into a control and an iron-supplemented group (n = 5). The treated group was supplemented with oral ferrous sulphate monohydrate (720 ppm of iron), whereas the control group received 320 ppm of iron daily for 15 d. Fecal samples were collected before and 5, 10, 15, and 30 d after supplementation and frozen at -80°C. DNA was sequenced using an Illumina MiSeq platform and data were analyzed using the software Mothur and linear discriminant analysis (LDA) effect size (LEfSe). Iron supplementation caused no change in the overall composition of the fecal microbiota, but some minor changes were observed in the low-abundant bacteria, as well as an increased alpha diversity after 15 d of supplementation. Significant differences in community composition of the fecal microbiota over time were observed in both groups, highlighting the importance of a control group, as there are variables that cannot be controlled in microbiome studies.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"87 2","pages":"97-104"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10069153/pdf/cjvr_02_97.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41124126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Administration safety of medical-grade honey (MGH) in septic synovial structures in horses: 3 cases. 医用蜂蜜（MGH）治疗马脓毒症滑膜结构的给药安全性：3例。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2023-04-01

Janine A Terschuur, Richard P C Coomer, Shaun A McKane

{"title":"Administration safety of medical-grade honey (MGH) in septic synovial structures in horses: 3 cases.","authors":"Janine A Terschuur, Richard P C Coomer, Shaun A McKane","doi":"","DOIUrl":"","url":null,"abstract":"The objective of this study was to investigate the postoperative use of intrasynovial honey as an antimicrobial after treatment for synovial sepsis in horses. One colt and 2 mares were presented with acute lameness, with or without an associated wound. All 3 cases were initially managed with surgical endoscopic or tenoscopic debridement and lavage for treatment of different synovial structures. Collection of synovial fluid was consistent with synovial sepsis and this was diagnosed in each case. All horses subsequently underwent arthroscopic lavage under general anesthetic and intraarticular or intrathecal medical-grade honey (MGH) was then instilled. All 3 cases recovered well and were free from lameness at all gaits. Although there is extensive research about the antimicrobial properties of honey and growing interest in the biocompatibility of honey in joints with the use of honey hydrogels in human medicine, the research in veterinary medicine is lacking. There are studies describing the antimicrobial properties of honey in healing wounds in horses, but there are no published studies describing the use of honey within a synovial structure. Further research is necessary to assess the biocompatibility of honey in equine articular cartilage. In the cases described in this article, the use of honey demonstrated a safe adjunctive therapy after conventional surgical treatment for septic arthritis.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"87 2","pages":"153-156"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10069145/pdf/cjvr_02_153.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41174637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of freezing and multiple freeze-thaw cycles on Brix refractometry estimates of immunoglobulin concentration in beef cattle colostrum. 冷冻和多次冻融循环对Brix折射法估计肉牛初乳中免疫球蛋白浓度的影响。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2023-04-01

Jacqueline M Stalker, Lisa Gamsjäger, Jennifer M Pearson, Douglas W Morck, M Claire Windeyer

{"title":"The impact of freezing and multiple freeze-thaw cycles on Brix refractometry estimates of immunoglobulin concentration in beef cattle colostrum.","authors":"Jacqueline M Stalker, Lisa Gamsjäger, Jennifer M Pearson, Douglas W Morck, M Claire Windeyer","doi":"","DOIUrl":"","url":null,"abstract":"Evaluation of immunoglobulin G (IgG) concentration in colostrum is important to guide on-farm management. Studies have shown that digital Brix refractometry accurately estimates colostrum IgG concentration in both dairy and beef cattle colostrum. Colostrum is often frozen in both clinical and research settings. The implications of this freezing on the accuracy of Brix refractometry measurements are largely unknown. The first objective of this study was to evaluate the agreement between digital Brix percentage measurements of IgG in beef cattle colostrum taken before and after different durations of freezing. The second objective was to evaluate the effects of multiple freeze-thaw (FT) cycles on Brix percentage measurements of IgG in beef cattle colostrum. There was good agreement between Brix percentages in fresh colostrum and after short (2 to 8 d), medium (4 to 7 mo), and long (3 y) periods of freezing (concordance correlation coefficient: 0.95, 0.96, and 0.96, respectively). Although there was no significant change in mean Brix percentages over 2 FT cycles (P > 0.05), mean Brix percentages decreased with 3 FT cycles (P = 0.017). Samples from the fourth and fifth FT cycles were observably coagulated, and these measurements were therefore deemed inaccurate. Data from this study indicate that freezing had minimal impact on digital Brix refractometer estimates of IgG concentration in beef cattle colostrum, but that samples stored for future testing should not undergo more than 2 FT cycles.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"87 2","pages":"146-152"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10069141/pdf/cjvr_02_146.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41175349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seneca Valley virus induces proinflammatory cytokine and chemokine response in vitro. Seneca Valley病毒在体外诱导促炎细胞因子和趋化因子反应。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2023-04-01

Jianguo Dong, Mingrui Chen, Linyang Yu, Dan Rao, Ning Zhang, Feng Cong

引用次数: 0

Comparison of surgeon experience using simple interrupted and simple continuous suture patterns in intestinal resection and anastomosis. 在肠切除和吻合中使用简单间断和简单连续缝合模式的外科医生经验比较。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2022-07-01

Christina M Fruehwald, Penny J Regier, Kaitlyn M Mullen, Monica Waln, Kaitlyn L McNamara, James Colee

{"title":"Comparison of surgeon experience using simple interrupted and simple continuous suture patterns in intestinal resection and anastomosis.","authors":"Christina M Fruehwald, Penny J Regier, Kaitlyn M Mullen, Monica Waln, Kaitlyn L McNamara, James Colee","doi":"","DOIUrl":"","url":null,"abstract":"This experimental study compared leak pressures and completion time of intestinal anastomoses performed by novice veterinarians and a Board-certified surgeon using simple interrupted and simple continuous suture patterns. Grossly normal jejunal segments (n = 108) from 6 fresh canine cadavers were used to harvest 8-cm cooled canine cadaveric jejunal segments that were randomly assigned to a control group (12 segments) and 4 treatment groups (24 segments/group, 12 constructs/group): i) simple interrupted anastomoses performed by a Board-certified surgeon (BSI); ii) simple continuous anastomoses performed by a Board-certified surgeon (BSC); iii) simple interrupted anastomoses performed by novice veterinarians (NSI); and iv) simple continuous anastomoses performed by novice veterinarians (NSC). Median (range) initial leak pressure (ILP) for control was 400.2 mmHg (226.0 to 500.0 mmHg), BSI 37.4 (14.4 to 124.0), BSC 32.5 (13.4 to 91.0), NSI 36.5 (22.9 to 62.0), and NSC 47.5 (8.9 to 120.0). No difference was noted between experience (P = 0.73, P = 0.53), suture technique (P = 0.07, P = 0.38), or across treatment groups (P = 0.17, P = 0.94), for ILP or MIP (maximum intraluminal pressure), respectively. Time to construct completion differed based on suture technique (P < 0.0001) and experience (P < 0.0001). The median and mean ILP of all anastomoses exceeded physiologic intraluminal peristaltic pressures. Simple continuous anastomoses were faster to complete overall. Both handsewn anastomosis techniques are appropriate for intestinal anastomoses.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"86 3","pages":"165-171"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251800/pdf/cjvr_03_165.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41158150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CONSORT-guided, randomized, double-blind, controlled pilot clinical trial of inhaled lidocaine for the treatment of equine asthma. CONSORT指导、随机、双盲、对照的吸入利多卡因治疗马哮喘的中试临床试验。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2022-04-01

Ananya Mahalingam-Dhingra, Melissa R Mazan, Daniela Bedenice, Michelle Ceresia, Jill Minuto, Edward F Deveney

{"title":"A CONSORT-guided, randomized, double-blind, controlled pilot clinical trial of inhaled lidocaine for the treatment of equine asthma.","authors":"Ananya Mahalingam-Dhingra, Melissa R Mazan, Daniela Bedenice, Michelle Ceresia, Jill Minuto, Edward F Deveney","doi":"","DOIUrl":"","url":null,"abstract":"There are limited options for treatment of the common disease, equine asthma. The aim of this study was to estimate the feasibility and potential efficacy of using nebulized lidocaine for treating equine asthma, while at the same time treating a separate cohort of asthmatic horses with inhaled budesonide. Nineteen horses with a history consistent with equine asthma were recruited from our referral population for a double-blind, randomized, controlled pilot clinical trial using Consolidated Standards of Reporting Trials (CONSORT) guidelines. After screening, 16 horses met the inclusion criteria for equine asthma and 13 horses actually completed the study. Horses were treated by their owners at home for 14 d before returning to our hospital for follow-up assessment. Interventions consisted of nebulization q12h for 14 d with 1.0 mg/kg body weight (BW) of lidocaine or corticosteroid treatment (nebulized budesonide 1 μg/kg, q12h). Clinical and tracheal mucus score, pulmonary function testing, and respiratory secretion cytology were assessed after 2 weeks of treatment to determine the outcome. Both lidocaine and budesonide cohorts had significant decreases (P < 0.05) in clinical score; the lidocaine cohort showed a significant decrease in bronchoalveolar lavage (BAL) neutrophil percentage and tracheal mucus score. Neither treatment resulted in significant changes in lung function parameters. No adverse events occurred. Lidocaine may be an effective and safe treatment for equine asthma in horses that cannot tolerate treatment with corticosteroids.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"86 2","pages":"116-124"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978287/pdf/cjvr_02_116.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41175739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of an energy additive on the metabolism of cattle. 能量添加剂对牛新陈代谢的影响。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-07-01

Elina Nafikova, Irina Mironova, Igor Gazeev, Dmitry Blagov, Azat Nigmatiyanov

{"title":"The effect of an energy additive on the metabolism of cattle.","authors":"Elina Nafikova, Irina Mironova, Igor Gazeev, Dmitry Blagov, Azat Nigmatiyanov","doi":"","DOIUrl":"","url":null,"abstract":"The objective of this study was to determine the effect of an energy additive on the metabolism of cattle. This article provides information on the analysis of the diet of young cattle calculated for when the animals were both indoors and outdoors. The ration was prepared for 40 heifers, divided into 4 groups consisting of 10 animals in each group. Three of these groups were fed different amounts of a high-energy additive, which was not fed to the control group. The effectiveness of the additive was analyzed according to the balance experiment and by calculating digestibility coefficients. It was determined that the percentage of nitrogen use in young animals was higher in the groups that were fed the additive than in the control group. Increasing the dose of the additive increased the level of nitrogen use. Comparative analysis of live weight indicated intergroup differences in favor of heifers in the groups that were fed the additive of 1.34% to 2.41% at the age of 9 mo; 2.51% to 4.16% at 12 mo; 3.14% to 5.46% at 15 mo; and 3.57% to 6.30% at 18 mo. The average daily growth dynamics indicated a gradual increase in all animals up to 15 mo, with a slight decrease by 18 mo of age. The difference among the groups ranged from 5.08% to 8.85% at 6 to 9 mo of age; 7.08% to 10.79% at 9 to 12 mo; 5.64% to 10.97% at 12 to 15 mo; and 6.05% to 11.11% at 18 mo. It was concluded that feeding the energy additive Tanrem to heifers increased their metabolism so that nitrogen use was improved, and feed was digested more efficiently, which in turn improved the growth of animals. Using an energy additive at the mid-range dose of 500 g a day per animal is recommended, since the effect was similar at the mid-range and maximum dosages.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 3","pages":"210-217"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243801/pdf/cjvr_03_210.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41166789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treatment of naturally occurring asthma with inhaled fluticasone or oral prednisolone: A randomized pilot trial. 吸入氟替卡松或口服泼尼松治疗自然发生的哮喘：一项随机试验。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2021-01-01

Michael Verschoor-Kirss, Elizabeth A Rozanski, Claire R Sharp, Trisha J Oura, Ashley Egan, Perry Bain, Joyce Knoll

{"title":"Treatment of naturally occurring asthma with inhaled fluticasone or oral prednisolone: A randomized pilot trial.","authors":"Michael Verschoor-Kirss, Elizabeth A Rozanski, Claire R Sharp, Trisha J Oura, Ashley Egan, Perry Bain, Joyce Knoll","doi":"","DOIUrl":"","url":null,"abstract":"The objective of this study was to compare inhaled glucocorticoids with oral glucocorticoids for treatment of naturally occurring feline asthma. Secondary goals were to evaluate serum allergy testing results in cats and to quantify the effect of an inhaled glucocorticoid (fluticasone) on glucose homeostasis. Nine cats with asthma were enrolled on the basis of clinical signs, thoracic radiographic findings, and airway eosinophilia. Cats were randomized and 4 cats were treated with oral glucocorticoids and 5 cats with inhaled glucocorticoids, with a 7-day course of oral glucocorticoids overlapping at the start of therapy. Cats were evaluated at baseline and at 8 wk with thoracic radiographs, bronchoalveolar lavage, lung function testing, and fructosamine levels. Serum allergen panels were evaluated. All cats were clinically normal after treatment and had significantly improved airway eosinophilia and decreased nucleated cell count. No improvement was seen in radiographic changes after treatment with either therapy. Oral, but not inhaled glucocorticoids, caused a decrease in airway resistance, although cats in the inhaled group had a higher baseline resistance than those in the oral group. Fructosamine levels did not change with treatment. Fifty percent of cats tested positive for immunoglobulin E (IgE) antibodies. Asthma is a heterogeneous condition; individual cats responded well to both oral and inhaled glucocorticoids. Ongoing evaluation of the potential underlying causes and therapeutic options is warranted with a larger group of cats.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"85 1","pages":"61-67"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747657/pdf/cjvr_01_61.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41166790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Duration of immunity after rabies vaccination in dogs: The Rabies Challenge Fund research study. 狂犬病疫苗接种后狗的免疫持续时间：狂犬病挑战基金研究。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2020-04-01

W Jean Dodds, Laurie J Larson, Kris L Christine, Ronald D Schultz

{"title":"Duration of immunity after rabies vaccination in dogs: The Rabies Challenge Fund research study.","authors":"W Jean Dodds, Laurie J Larson, Kris L Christine, Ronald D Schultz","doi":"","DOIUrl":"","url":null,"abstract":"A prospective study of 65 research beagles kept in a rabies-free environment was undertaken to determine the duration of immunity after they received licensed rabies vaccines. The eventual goal was to extend mandated rabies booster intervals to 5 or 7 years and help reduce the risk of vaccine-associated adverse events. Three groups of dogs were vaccinated with 1 of 2 commercial rabies vaccines or saline at 12 and 15 weeks of age. Beginning 5 years 5 months later, vaccinated and unvaccinated dogs were challenged with virulent rabies virus and observed for 90 days over a series of 3 trials. Humoral and cellular immune responses were examined by serology and flow cytometry. Brain tissue from all challenged dogs was tested for rabies virus. Challenge trial 1 was confounded due to insufficiently virulent virus. In trials 2 and 3 virulent challenge provided 100% mortality in controls. Vaccinate survival was 80% (4/5) after 6 years 7 months, 50% (6/12) after 7 years 1 month, and 20% (1/5) after 8years 0 months. Antibody responses 12 days post-challenge correlated strongly with survival. In a separate non-challenge trial, administration of either a recombinant or a killed rabies vaccine demonstrated memory antibody responses 6 years 1 month after initial vaccination compared with unvaccinated controls. Our data demonstrated that i) duration of immunity to rabies in vaccinated dogs extends beyond 3 years; ii) immunologic memory exists even in vaccinated dogs with serum antibody titer < 0.1 IU/mL; and iii) non-adjuvanted recombinant rabies vaccine induces excellent antibody responses in previously vaccinated dogs 14 days after administration.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"84 2","pages":"153-158"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088826/pdf/cjvr_02_153.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41165391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular variability of Streptococcus uberis isolates from intramammary infections in Canadian dairy farms from the Maritime region. 从加拿大滨海地区奶牛场乳房内感染中分离出的尤伯杯链球菌的分子变异性。

IF 0.8

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2019-07-01

Julian Reyes, Juan C Rodriguez-Lecompte, Adam Blanchard, J T McClure, Javier Sánchez

{"title":"Molecular variability of Streptococcus uberis isolates from intramammary infections in Canadian dairy farms from the Maritime region.","authors":"Julian Reyes, Juan C Rodriguez-Lecompte, Adam Blanchard, J T McClure, Javier Sánchez","doi":"","DOIUrl":"","url":null,"abstract":"The primary objective of this study was to explore the variability of Streptococcus uberis (S. uberis) isolates by extracting multilocus sequence typing (MLST) data from whole-genome sequencing. The secondary objective was to determine the distribution of the phenotypic antimicrobial resistance (AMR) and the associated AMR genes as well as the virulence gene profiles among sequence types (STs). Sixty-two isolates were recovered from 16 herds in 3 Canadian Maritime Provinces: New Brunswick (14.5%), Nova Scotia (48.3%), and Prince Edward Island (37.1%). Of these, 9, 30, and 23 were recovered from post-calving, lactational samples, and post-mastitis samples, respectively. These 62 S. uberis isolates belonged to 34 STs; 11 isolates were typed to 9 known STs and 51 isolates were classified as one of 25 new STs. Thirteen isolates were part of major clonal complexes (CCs). Post-mastitis isolates contained 10 unique STs, lactational isolates contained 11 unique STs, and post-calving isolates had 3 STs. Each farm had only 1 isolate that was a unique ST except for STs 233, 851, 855, 857, 864, and 866, which were found in multiple cows per herd on more than one farm. ST851 and ST857 were found in each of the 3 sample types, with ST857 found in cows from all 3 Maritime provinces. These results indicate that S. uberis is a diverse non-clonal pathogen with specific STs residing in clonal clusters, carrying multiple AMR genes and virulence, with a diverse phenotypic AMR.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"83 3","pages":"168-176"},"PeriodicalIF":0.8,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141629572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0