Canadian journal of veterinary research = Revue canadienne de recherche veterinaire最新文献_第4页

Production of a new tetravalent vaccine targeting fimbriae and enterotoxin of enterotoxigenic Escherichia coli. 针对肠毒性大肠杆菌的鞭毛和肠毒素生产新型四价疫苗。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2024-04-01

ChongLi Xu, Yuhan She, Fengyang Fu, ChongBo Xu

{"title":"Production of a new tetravalent vaccine targeting fimbriae and enterotoxin of enterotoxigenic Escherichia coli.","authors":"ChongLi Xu, Yuhan She, Fengyang Fu, ChongBo Xu","doi":"","DOIUrl":"","url":null,"abstract":"Enterotoxigenic Escherichia coli (ETEC) is an important type of pathogenic bacteria that causes diarrhea in pigs. The objective of this study was to prepare a novel tetravalent vaccine to effectively prevent piglet diarrhea caused by E. coli. In order to realize the production of K88ac-K99-ST1-LTB tetravalent inactivated vaccine, the biological characteristics, stability, preservation conditions, and safety of the recombinant strain BL21(DE3) (pXKKSL4) were studied, and the vaccine efficacy and minimum immune dose were measured. The results indicated that the biological characteristics, target protein expression, and immunogenicity of the 1st to 10th generations of the strain were stable. Therefore, the basic seed generation was preliminarily set as the 1st to 10th generations. The results of the efficacy tests showed that the immune protection rate could reach 90% with 1 minimum lethal dose (MLD) virulent strain attack in mice. The immunogenicity was stable, and the minimum immune dose was 0.1 mL per mouse. Our research showed that the genetically engineered vaccine developed in this way could prevent piglet diarrhea caused by enterotoxigenic E. coli through adhesin and enterotoxin. In order to realize industrial production of the vaccine as soon as possible, we conducted immunological tests and production process research on the constructed K88ac-K99-ST1-LTB tetravalent inactivated vaccine. The results of this study provide scientific experimental data for the commercial production of vaccines and lay a solid foundation for their industrial production.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"88 2","pages":"38-44"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11000427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140867930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of pathogens in honey bee colonies and association with clinical signs in southwestern Quebec, Canada. 加拿大魁北克省西南部蜜蜂群中病原体的流行情况及其与临床症状的关系。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2024-04-01

Gabrielle Claing, Pascal Dubreuil, Martine Bernier, Julie Ferland, Yvan L'Homme, Edisleidy Rodriguez, Julie Arsenault

{"title":"Prevalence of pathogens in honey bee colonies and association with clinical signs in southwestern Quebec, Canada.","authors":"Gabrielle Claing, Pascal Dubreuil, Martine Bernier, Julie Ferland, Yvan L'Homme, Edisleidy Rodriguez, Julie Arsenault","doi":"","DOIUrl":"","url":null,"abstract":"Honey bees can be affected by a variety of pathogens, which impacts their vital role as pollinators in agriculture. A cross-sectional study was conducted in southwestern Quebec to: i) estimate the prevalence of 11 bee pathogens; ii) assess the agreement between beekeeper suspicion of a disease and laboratory detection of the causative pathogen; and iii) explore the association between observed clinical signs and pathogen detection in a colony. A total of 242 colonies in 31 apiaries owned by 15 beekeepers was sampled in August 2017. The prevalence of Varroa destructor detection was estimated as 48% for colonies and 93% for apiaries. The apparent prevalence of colonies infected by Nosema spp. and Melissococcus plutonius was estimated as 40% and 21%, respectively. At least 180 colonies were tested by polymerase chain reaction (PCR) for deformed wing virus (DWV), acute-Kashmir-Israeli complex (AKI complex), and black queen cell virus (BQCV), which were detected in 33%, 9%, and 95% of colonies, respectively. Acarapis woodi, Paenibacillus larvae, and Aethina tumida were not detected. Varroasis was suspected by beekeepers in 14 of the 15 beekeeping operations in which the mite was detected. However, no correlation was found between suspected European foulbrood and detection of M. plutonius or between suspected nosemosis and detection of Nosema spp. Colony weakness was associated with Nosema spore counts of at least 0.5 × 106 per bee. Melissococcus plutonius was more frequently detected in colonies showing scattered brood.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"88 2","pages":"45-54"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11000428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140873843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of blood profiles between housed and grazing Korean indigenous cattle (Hanwoo). 韩国本土牛（Hanwoo）舍饲和放牧血液特征的比较。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2024-04-01

Youngjun Kim, Ji-Yeong Ku, Kwang-Man Park, Jonghun Baek, Kyoung-Seong Choi, Jinho Park

引用次数: 0

Comparison of estimations of urinary bladder volume in different scanning positions using 2D linear dimension formula and 3D bladder circumference tracing in client-owned cats. 使用二维线性维度公式和三维膀胱周径追踪法估算客户饲养的猫在不同扫描位置的膀胱容量的比较。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2024-04-01

Sabrina Ayoub, Xiu Ting Yiew, Gabrielle Monteith, Allan R Willms

{"title":"Comparison of estimations of urinary bladder volume in different scanning positions using 2D linear dimension formula and 3D bladder circumference tracing in client-owned cats.","authors":"Sabrina Ayoub, Xiu Ting Yiew, Gabrielle Monteith, Allan R Willms","doi":"","DOIUrl":"","url":null,"abstract":"Urinary bladder volume (UBV) can be estimated using point-of-care ultrasound. The purpose of this study was to compare 2 UBV estimation methods, i.e., three-dimensional (3D) bladder circumference tracing and 2-dimensional (2D) linear bladder dimension formula, against actual bladder volumes in awake client-owned cats and identify the best scanning position for UBV estimations. Up to 3 paired sets of orthogonal longitudinal and transverse bladder ultrasound images were acquired by a trained clinician from 21 cats positioned in dorsal, right lateral, and left lateral recumbency. UBV estimation was performed with these images by 2 different observers using both methods. Actual bladder volumes were measured through urethral catheterization and compared to the estimated UBV using Lin's concordance correlation coefficient and Bland-Altman analyses. Considering all positions, both methods showed substantial strength-of-agreement with actual bladder volumes; the 3D bladder circumference method (ρc = 0.963, 95% CI: 0.952 to 0.974) with a significant median bias of -4.08 mL (P < 0.001, IQR -7.63 to -0.68 mL, LOA -48.55 to 21.75 mL) and the 2D linear dimension method (ρc = 0.974, 95% CI: 0.966 to 0.982) with a median bias of -0.82 mL (P = 0.686, IQR -3.89 to 4.05 mL, LOA -35.23 to 35.21 mL). Scanning in left lateral recumbency provided the strongest strengths-of-agreement and precision against actual bladder volumes for both methods. Regardless of scanning positions, the 2D linear dimension method is more accurate than the 3D bladder circumference method, although both methods are imprecise with increasing volumes and UBV assessment through urinary catheterization remains the gold standard.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"88 2","pages":"55-65"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11000429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ability of unsterilized recycled manure solids bedding to support growth of Klebsiella pneumoniae and Escherichia coli. 未经消毒的回收粪便固体垫料支持肺炎克雷伯氏菌和大肠埃希氏菌生长的能力。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2024-01-01

Annie Fréchette, Mylène Généreux, Gilles Fecteau, Caroline Côté, Simon Dufour

{"title":"Ability of unsterilized recycled manure solids bedding to support growth of Klebsiella pneumoniae and Escherichia coli.","authors":"Annie Fréchette, Mylène Généreux, Gilles Fecteau, Caroline Côté, Simon Dufour","doi":"","DOIUrl":"","url":null,"abstract":"Although recycled manure solids (RMS) bedding is used on dairy farms, it could allow bacterial growth when contaminated by feces and thus increase the incidence of clinical mastitis in cows. The objective of this study was to describe bacterial growth in three different types of RMS bedding, as well as in sand, when samples were experimentally inoculated with Escherichia coli and Klebsiella pneumoniae. Two 3-day trials were conducted, during which treatments included inoculating bedding samples with E. coli and K. pneumoniae, as well as no inoculation. The trial was repeated 3 times for each bedding sample on each day. Samples were incubated at 15°C for 3 d and bacterial counts were measured every day. After inoculation, there was no significant K. pneumoniae or E. coli growth phase during the trial in those RMS samples that were prepared either in a container or in a heap. Recycled manure solids and sand samples prepared in a rotary drum, however, showed a similar active growth phase of K. pneumoniae during the first 24 h of the trial. Moreover, a significant E. coli growth phase was observed in the samples of sand bedding in the first 24 h. The 3 different types of RMS bedding samples did not react in a similar manner to coliform inoculation. No active growth phase was observed in bedding samples already containing a high bacterial concentration following inoculation with coliforms.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"88 1","pages":"12-18"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10782466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgment of reviewers/translators. 鸣谢审稿人/译者。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2024-01-01

引用次数: 0

Metabolic response of broiler chickens to different doses of Enterococcus faecium ICIS 96 in the diet. 肉鸡对日粮中不同剂量的肠球菌 ICIS 96 的代谢反应。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2024-01-01

Elena Kochkina, Alfia Andreeva, Alexey Torshkov, Veronika Dymova, Oleg Altynbekov, Mariia Sycheva

{"title":"Metabolic response of broiler chickens to different doses of Enterococcus faecium ICIS 96 in the diet.","authors":"Elena Kochkina, Alfia Andreeva, Alexey Torshkov, Veronika Dymova, Oleg Altynbekov, Mariia Sycheva","doi":"","DOIUrl":"","url":null,"abstract":"The competitiveness and profitability of the poultry industry in market conditions are hampered by growing problems with the safety and quality of poultry meat. The use of targeted microbial preparations can assist in resolving these problems. Numerous studies of the properties of bacteria of the genus Enterococcus have shown their effectiveness in the practice of poultry farming. The objectives of this study were to identify the effect of various doses of Enterococcus faecium ICIS 96 added to the diet of broiler chickens on their metabolism and to evaluate the productive qualities of the chickens when different doses are used. The experiment was carried out on 72 Cobb-500 cross broiler chickens, divided into 3 groups. Chickens in the first group received a suspension of E. faecium ICIS 96 containing 1 × 108 cells in 1 mL of sterile saline, at a dose of 0.1 mL per 1 kg of live weight. The second group of chickens received a double dose of enterococcus, that is 0.2 mL of the suspension per 1 kg of live weight per day. The results demonstrated that 0.1 mL of a 1 × 108 per mL enterococcus culture suspension in the poultry diet per 1 kg of live weight per day intensified metabolism and increased the live weight of chickens after 40 d of treatment.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"88 1","pages":"19-23"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10782467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective study of perioperative antimicrobial use in horses undergoing elective laparoscopy at a single institution. 对一家医疗机构接受择期腹腔镜手术的马匹围手术期抗菌药使用情况的回顾性研究。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2024-01-01

Lucciana Recchi, Nicola Cribb, Nathalie Côté, Marie-Soleil Dubois, Judith Koenig, Alexander Valverde, Gabrielle Monteith

{"title":"Retrospective study of perioperative antimicrobial use in horses undergoing elective laparoscopy at a single institution.","authors":"Lucciana Recchi, Nicola Cribb, Nathalie Côté, Marie-Soleil Dubois, Judith Koenig, Alexander Valverde, Gabrielle Monteith","doi":"","DOIUrl":"","url":null,"abstract":"Antimicrobial stewardship has shown significant development in recent years. Perioperative prophylaxis accounts for a substantial volume of antimicrobial use and is a field in which improvements can likely be made. The objective of this study was to evaluate practices associated with perioperative antimicrobial use in equine elective laparoscopy at a single institution over a 21-year period and to determine whether antimicrobial therapy influenced the occurrence of postoperative complications. Medical records of horses that underwent elective laparoscopy at a teaching hospital from January 2000 to September 2021 were reviewed. Data obtained included signalment, surgeon, type and duration of procedure, perioperative antimicrobial use, and intraoperative and postoperative complications. Exact univariate logistic regression was used to explore the association between possible risk factors and occurrence of postoperative complications, as well as the association between year of presentation and time of antimicrobial administration. Duration of surgery was log-transformed to meet assumption of normality, followed by analysis of variance (ANOVA) to compare mean surgery time per procedure and postoperative complications. Significance was set at P < 0.05. Sixty horses met the inclusion criteria. All horses received antimicrobial prophylaxis, but none received intraoperative redosing. Only 13 horses (26%) received antimicrobials within 60 min of the first incision. Time of administration improved with each year of the study (P = 0.005). Only 17 horses (28%) received antimicrobials for less than 24 h, but median duration of antimicrobial therapy was 1.25 d (range: 0.25 to 10 d). Antimicrobial use practices at this institution differed from general recommendations for optimal perioperative prophylaxis, which suggests that intervention is required.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"88 1","pages":"24-29"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10782465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of a farm- and province-level swine flow simulation model using discrete events and Ontario swine farm and provincial input data. 利用离散事件和安大略省猪场及省输入数据，开发并验证猪场和省一级的猪流量模拟模型。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2024-01-01

Maggie Henry, Wade McDonald, Robert M Friendship, Amy L Greer, Zvonimir Poljak

{"title":"Development and validation of a farm- and province-level swine flow simulation model using discrete events and Ontario swine farm and provincial input data.","authors":"Maggie Henry, Wade McDonald, Robert M Friendship, Amy L Greer, Zvonimir Poljak","doi":"","DOIUrl":"","url":null,"abstract":"Infectious disease events can cause disruptions in service-based and agricultural industries. The list of possible events is long and varies from the incursion or emergence of a reportable animal pathogen to the recently documented interruptions caused by the COVID-19 pandemic. There is a need to develop models that can determine the impact of pathogens and mitigation measures on populations that are not directly affected by the pathogen in the case of a reportable disease, particularly when the health and welfare of these populations could be affected due to resulting disruptions in trade and supply chains. The primary objective of this study was to develop a discrete-event simulation (DES) model of swine production, including pork processing, for scenarios without major disruptions, which could be scaled from the level of an individual farm to the entire province of Ontario, Canada. The secondary objective was to validate the developed simulation against observed farm- and province-level statistics. A weekly discrete-event simulation consisting of 3 connected areas (a sow farm, a pig farm, and abattoirs) was developed using AnyLogic modelling software. Using Mann-Whitney tests, model outputs representative of the standard industry statistics were compared to data from 6 individual farms separately, as well as to provincial data from Ontario. A scalable discrete-event simulation of the swine production system for typical scenarios was accomplished. The model outputs were consistent with individual farm and industry statistics. As such, the model can be used to simulate swine production at distinct levels and could be further modified to represent swine marketing in other provinces or internationally.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"88 1","pages":"3-11"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10782463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complications associated with a flash glucose monitoring system in diabetic dogs. 糖尿病犬闪光血糖监测系统的并发症。

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2023-10-01

Carson Campbell, Adam Shoelson, Orla Mahony

{"title":"Complications associated with a flash glucose monitoring system in diabetic dogs.","authors":"Carson Campbell, Adam Shoelson, Orla Mahony","doi":"","DOIUrl":"","url":null,"abstract":"Interstitial glucose monitoring systems are commonly being used in diabetic dogs. The aim of this study was to document the incidence of complications associated with the use of a flash glucose monitoring system (FGMS) in dogs. Medical records of dogs that had placement of a 14-day FGMS during a 1-year period were reviewed. Data retrieved included the number of days the sensor remained attached and functional, sensor detachment, sensor failure prior to the end of the 14-day monitoring period, and dermatologic changes at the sensor site. Descriptive statistics were used to characterize the data. Thirty-four dogs had FGMSs placed. Most [32/34 (94%)] sensors were placed over the dorsolateral aspect of the thorax caudal to the scapula. Twenty-four sensors (71%) remained attached for the full 14 days. Incidence of complications associated with FGMS use was 13/34 (38%). The most frequent complication was mild dermatologic changes at the sensor site [6/34 (18%)]. Erythema and crusting at the attachment site were common and could be related to contact dermatitis, hypersensitivity, or skin preparation prior to placement. Flash glucose monitoring systems are safe in dogs, although there are some potential complications that should be discussed with dog owners.","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"87 4","pages":"260-264"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41149675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0