Annie Fréchette, Mylène Généreux, Gilles Fecteau, Caroline Côté, Simon Dufour
{"title":"Ability of unsterilized recycled manure solids bedding to support growth of <i>Klebsiella pneumoniae</i> and <i>Escherichia coli</i>.","authors":"Annie Fréchette, Mylène Généreux, Gilles Fecteau, Caroline Côté, Simon Dufour","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Although recycled manure solids (RMS) bedding is used on dairy farms, it could allow bacterial growth when contaminated by feces and thus increase the incidence of clinical mastitis in cows. The objective of this study was to describe bacterial growth in three different types of RMS bedding, as well as in sand, when samples were experimentally inoculated with <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i>. Two 3-day trials were conducted, during which treatments included inoculating bedding samples with <i>E. coli</i> and <i>K. pneumoniae</i>, as well as no inoculation. The trial was repeated 3 times for each bedding sample on each day. Samples were incubated at 15°C for 3 d and bacterial counts were measured every day. After inoculation, there was no significant <i>K. pneumoniae</i> or <i>E. coli</i> growth phase during the trial in those RMS samples that were prepared either in a container or in a heap. Recycled manure solids and sand samples prepared in a rotary drum, however, showed a similar active growth phase of <i>K. pneumoniae</i> during the first 24 h of the trial. Moreover, a significant <i>E. coli</i> growth phase was observed in the samples of sand bedding in the first 24 h. The 3 different types of RMS bedding samples did not react in a similar manner to coliform inoculation. No active growth phase was observed in bedding samples already containing a high bacterial concentration following inoculation with coliforms.</p>","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"88 1","pages":"12-18"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10782466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Acknowledgment of reviewers/translators.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"88 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10782464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Metabolic response of broiler chickens to different doses of <i>Enterococcus faecium</i> ICIS 96 in the diet.","authors":"Elena Kochkina, Alfia Andreeva, Alexey Torshkov, Veronika Dymova, Oleg Altynbekov, Mariia Sycheva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The competitiveness and profitability of the poultry industry in market conditions are hampered by growing problems with the safety and quality of poultry meat. The use of targeted microbial preparations can assist in resolving these problems. Numerous studies of the properties of bacteria of the genus <i>Enterococcus</i> have shown their effectiveness in the practice of poultry farming. The objectives of this study were to identify the effect of various doses of <i>Enterococcus faecium</i> ICIS 96 added to the diet of broiler chickens on their metabolism and to evaluate the productive qualities of the chickens when different doses are used. The experiment was carried out on 72 Cobb-500 cross broiler chickens, divided into 3 groups. Chickens in the first group received a suspension of <i>E. faecium</i> ICIS 96 containing 1 × 10<sup>8</sup> cells in 1 mL of sterile saline, at a dose of 0.1 mL per 1 kg of live weight. The second group of chickens received a double dose of enterococcus, that is 0.2 mL of the suspension per 1 kg of live weight per day. The results demonstrated that 0.1 mL of a 1 × 10<sup>8</sup> per mL enterococcus culture suspension in the poultry diet per 1 kg of live weight per day intensified metabolism and increased the live weight of chickens after 40 d of treatment.</p>","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"88 1","pages":"19-23"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10782467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Retrospective study of perioperative antimicrobial use in horses undergoing elective laparoscopy at a single institution.","authors":"Lucciana Recchi, Nicola Cribb, Nathalie Côté, Marie-Soleil Dubois, Judith Koenig, Alexander Valverde, Gabrielle Monteith","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Antimicrobial stewardship has shown significant development in recent years. Perioperative prophylaxis accounts for a substantial volume of antimicrobial use and is a field in which improvements can likely be made. The objective of this study was to evaluate practices associated with perioperative antimicrobial use in equine elective laparoscopy at a single institution over a 21-year period and to determine whether antimicrobial therapy influenced the occurrence of postoperative complications. Medical records of horses that underwent elective laparoscopy at a teaching hospital from January 2000 to September 2021 were reviewed. Data obtained included signalment, surgeon, type and duration of procedure, perioperative antimicrobial use, and intraoperative and postoperative complications. Exact univariate logistic regression was used to explore the association between possible risk factors and occurrence of postoperative complications, as well as the association between year of presentation and time of antimicrobial administration. Duration of surgery was log-transformed to meet assumption of normality, followed by analysis of variance (ANOVA) to compare mean surgery time per procedure and postoperative complications. Significance was set at <i>P</i> < 0.05. Sixty horses met the inclusion criteria. All horses received antimicrobial prophylaxis, but none received intraoperative redosing. Only 13 horses (26%) received antimicrobials within 60 min of the first incision. Time of administration improved with each year of the study (<i>P</i> = 0.005). Only 17 horses (28%) received antimicrobials for less than 24 h, but median duration of antimicrobial therapy was 1.25 d (range: 0.25 to 10 d). Antimicrobial use practices at this institution differed from general recommendations for optimal perioperative prophylaxis, which suggests that intervention is required.</p>","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"88 1","pages":"24-29"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10782465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maggie Henry, Wade McDonald, Robert M Friendship, Amy L Greer, Zvonimir Poljak
{"title":"Development and validation of a farm- and province-level swine flow simulation model using discrete events and Ontario swine farm and provincial input data.","authors":"Maggie Henry, Wade McDonald, Robert M Friendship, Amy L Greer, Zvonimir Poljak","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Infectious disease events can cause disruptions in service-based and agricultural industries. The list of possible events is long and varies from the incursion or emergence of a reportable animal pathogen to the recently documented interruptions caused by the COVID-19 pandemic. There is a need to develop models that can determine the impact of pathogens and mitigation measures on populations that are not directly affected by the pathogen in the case of a reportable disease, particularly when the health and welfare of these populations could be affected due to resulting disruptions in trade and supply chains. The primary objective of this study was to develop a discrete-event simulation (DES) model of swine production, including pork processing, for scenarios without major disruptions, which could be scaled from the level of an individual farm to the entire province of Ontario, Canada. The secondary objective was to validate the developed simulation against observed farm- and province-level statistics. A weekly discrete-event simulation consisting of 3 connected areas (a sow farm, a pig farm, and abattoirs) was developed using AnyLogic modelling software. Using Mann-Whitney tests, model outputs representative of the standard industry statistics were compared to data from 6 individual farms separately, as well as to provincial data from Ontario. A scalable discrete-event simulation of the swine production system for typical scenarios was accomplished. The model outputs were consistent with individual farm and industry statistics. As such, the model can be used to simulate swine production at distinct levels and could be further modified to represent swine marketing in other provinces or internationally.</p>","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"88 1","pages":"3-11"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10782463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Complications associated with a flash glucose monitoring system in diabetic dogs.","authors":"Carson Campbell, Adam Shoelson, Orla Mahony","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Interstitial glucose monitoring systems are commonly being used in diabetic dogs. The aim of this study was to document the incidence of complications associated with the use of a flash glucose monitoring system (FGMS) in dogs. Medical records of dogs that had placement of a 14-day FGMS during a 1-year period were reviewed. Data retrieved included the number of days the sensor remained attached and functional, sensor detachment, sensor failure prior to the end of the 14-day monitoring period, and dermatologic changes at the sensor site. Descriptive statistics were used to characterize the data. Thirty-four dogs had FGMSs placed. Most [32/34 (94%)] sensors were placed over the dorsolateral aspect of the thorax caudal to the scapula. Twenty-four sensors (71%) remained attached for the full 14 days. Incidence of complications associated with FGMS use was 13/34 (38%). The most frequent complication was mild dermatologic changes at the sensor site [6/34 (18%)]. Erythema and crusting at the attachment site were common and could be related to contact dermatitis, hypersensitivity, or skin preparation prior to placement. Flash glucose monitoring systems are safe in dogs, although there are some potential complications that should be discussed with dog owners.</p>","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"87 4","pages":"260-264"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41149675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Francisco Aranda-Jiménez, Carlos Martin-Bernal, Daniel Hernández-León, Jose Luis Fontalba-Navas, Ignacio Calvo
{"title":"Short- and long-term outcomes of subtotal ear canal ablation and lateral bulla osteotomy in French bulldogs.","authors":"Francisco Aranda-Jiménez, Carlos Martin-Bernal, Daniel Hernández-León, Jose Luis Fontalba-Navas, Ignacio Calvo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The objective of this retrospective study was to report the overall peri-operative complications and short- to long-term outcomes of subtotal ear canal ablation and lateral bulla osteotomy in French bulldogs with otitis media. Medical records from 2018 to 2021 were reviewed for preoperative neurological signs, surgical technique, postoperative complications, and long-term outcomes. Postoperative complications, such as a head tilt, nystagmus, ataxia, facial nerve dysfunction, as well as skin or wound infection, were recorded. Eighteen client-owned dogs (20 ears) were included in the study. Postoperative complications were observed in 5/18 dogs (6/20 ears) with 2 of them being considered major complications. Referring veterinarians and owner telephone follow-up (mean: 33 mo, range: 17 to 56 mo) were obtained for all patients. Total recovery was reported in 9/18 dogs (11/20 ears) with a postoperative improvement of the vestibular signs and facial nerve paresis within 8 wk. All dogs maintained the pinnas' natural, erect morphology, and movement.</p>","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"87 4","pages":"277-281"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41168777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alison A Cain, Garrett J Davis, Spencer Davis, Richard P Bastian, Veronica Marquez, Hollyn Probasco, Emma Desantis
{"title":"Infrared thermography as a diagnostic tool to detect cranial cruciate ligament deficiency in dogs.","authors":"Alison A Cain, Garrett J Davis, Spencer Davis, Richard P Bastian, Veronica Marquez, Hollyn Probasco, Emma Desantis","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cranial cruciate ligament (CCL) disease is the leading cause of canine lameness. Diagnosis is based on physical examination and diagnostic imaging findings. Limitations of diagnostic modalities, including patient temperament, cost, availability, and need for general anesthesia, preclude their use for many patients. Infrared thermography is an easy, non-invasive diagnostic screening technique with use in both human and veterinary musculoskeletal conditions. The objective of this study was to investigate the utility of infrared thermography to diagnose CCL disease in a large population of client-owned dogs and investigate for correlations between ability of thermography to diagnose CCL deficiency and recorded explanatory factors. A commercial grade thermal imaging camera was used to collect preoperative images of both stifles in 50 dogs with surgically confirmed CCL rupture in one stifle and a normal opposite stifle. The average maximum temperatures of the affected and unaffected stifles were collected from these images and used for statistical analysis. Infrared thermography was not successful in differentiating between CCL-deficient and unaffected stifles in this cohort. No significant differences in average maximal temperature were observed between stifles when comparing to explanatory factors or variables. Use of infrared thermography should not be relied upon to diagnose canine CCL disease.</p>","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"87 4","pages":"290-296"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41171592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphine concentrations in distal thoracic limb synovial fluid following intravenous regional limb perfusion in horses.","authors":"Alexander Valverde, Nicola Cribb, Luis Arroyo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Twelve adult horses were randomly assigned to 2 groups in a prospective experimental trial. A pneumatic tourniquet (425 mmHg) was placed, under sedation, proximal to the carpus on one randomly chosen thoracic limb. A cephalic vein catheter was placed distal to the tourniquet to establish an intravenous regional limb perfusion technique (IVRLP) with morphine (0.1 mg/kg) diluted with saline 0.9% to 0.1 mL/kg, and the tourniquet left in place for 30 minutes. Horses were euthanized at 1 h (Group I) or 6 h (Group II) following the IVRLP and synovial fluid from the radiocarpal, intercarpal, metacarpophalangeal, distal interphalangeal, and digital flexor tendon sheath was obtained from the injected and contralateral (control) limb immediately after. Morphine concentrations were determined using liquid chromatography-mass spectrometry. An unpaired <i>t</i>-test was used to compare morphine concentrations from both groups (<i>P</i> < 0.05). Synovial morphine concentrations were detected in the injected limb in all horses in Group I, except in one horse in the radiocarpal joint, and detected in 5 of 6 horses in Group II. Mean synovial concentrations ranged between 301.8 ± 192.3 and 608 ± 446.6 ng/mL in Group I and between 27.0 ± 17.7 and 136.8 ± 103.6 ng/mL in Group II and were significantly higher in Group I between paired anatomical sites of both groups for the radiocarpal and distal interphalangeal joints, and the digital flexor tendon sheath. In conclusion, concentrations of morphine after IVRLP can be detected in synovial fluid distal to the tourniquet at 1 and 6 hours in horses.</p>","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"87 4","pages":"254-259"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41164305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jeongmin Suh, Sehyeong Ham, Taehwan Oh, Chanhee Chae
{"title":"Cross-protection of a porcine circovirus types 2a/b (PCV-2a/b) and <i>Mycoplasma hyopneumoniae</i> trivalent vaccine against a dual PCV-2e and <i>Mycoplasma hyopneumoniae</i> challenge.","authors":"Jeongmin Suh, Sehyeong Ham, Taehwan Oh, Chanhee Chae","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The purpose of this experimental study was to determine the cross-protection of a new trivalent vaccine containing porcine circovirus types 2a/b (PCV-2a/b) and <i>Mycoplasma hyopneumoniae.</i> Pigs were vaccinated intramuscularly at 21 days of age, then challenged at 42 days of age with a dual PCV-2e and <i>M. hyopneumoniae</i> challenge. Growth performance was significantly improved during the experimental period (21 to 63 days of age) in vaccinated-challenged pigs compared to unvaccinated-challenged pigs. Pigs that were vaccinated and challenged elicited a significant amount of PCV-2e- and <i>M. hyopneumoniae</i>-specific interferon-γ secreting cells (IFN-γ-SC) and reduced the levels of PCV-2e viremia and laryngeal shedding. The results of the present study demonstrated that a trivalent vaccine provided cross-protection against a dual PCV-2e and <i>M. hyopneumoniae</i> challenge.</p>","PeriodicalId":93919,"journal":{"name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","volume":"87 4","pages":"297-302"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41172917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}