Bioscience of microbiota, food and health最新文献

Utilization of the enzyme pullulanase and Lactobacillus acidophilus as to produce synbiotic rice yogurt cakes. 利用普鲁兰酶和嗜酸乳杆菌生产合成米酸奶蛋糕。

IF 2.1

Bioscience of microbiota, food and health Pub Date : 2026-01-01 Epub Date: 2026-05-12 DOI: 10.12938/bmfh.2025-112

Anh Hoang Nguyen, Phat Thuan Nguyen, Duy Doan Nguyen LE

{"title":"Utilization of the enzyme pullulanase and Lactobacillus acidophilus as to produce synbiotic rice yogurt cakes.","authors":"Anh Hoang Nguyen, Phat Thuan Nguyen, Duy Doan Nguyen LE","doi":"10.12938/bmfh.2025-112","DOIUrl":"10.12938/bmfh.2025-112","url":null,"abstract":"This study developed a synbiotic rice yogurt cake with a medium glycemic index by combining resistant starch-enhanced rice with Lactobacillus acidophilus. The effects of substrate concentration (20-40%), pullulanase enzyme activity (20-40 U/g), and hydrolysis time (4-8 hr) on resistant starch content were investigated. At a substrate concentration of 30%, an enzyme activity of 30 U/g, and a hydrolysis time of 6 hr, the optimal resistant starch content was 14.54 ± 0.96%. After being treated to enhance its resistant starch content, IR504 rice was mixed with milk and processed to produce rice milk. The rice milk was fermented for 12 hr at 37°C with a 3% inoculum ratio, yielding the highest L. acidophilus count of 5.62 × 107 CFU/g. After freeze-drying, the synbiotic rice yogurt cake contained 3.8 × 107 CFU/g L. acidophilus and 3.55 g/100 g resistant starch. Nutritional analysis showed 82.4 g carbohydrates, 6.78 g lipids, 8.90 g proteins, and 1.05% moisture per 100 g. This product achieved a lower estimated glycemic index of 69.32 ± 0.534 compared with non-resistant starch (77.74 ± 0.369). Microbiological tests confirmed safety standards were met, with undetectable Enterobacteriaceae (<10 CFU/g), Listeria monocytogenes (undetectable/25 g), and undetectable total mold. The predicted shelf life of the synbiotic rice yogurt cake was approximately 198 days under typical storage conditions at 30°C and L. acidophilus count of 4.8 × 106 CFU/g. Therefore, the synbiotic rice yogurt cake can be used as a product supporting glycemic control while simultaneously providing a substrate for beneficial gut bacteria.","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 3","pages":"204-216"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148378494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiogeographic insights as keys to understanding personalized gut microbiota responses: the role of Bifidobacterium residing on intestinal starch granules. 微生物地理学的见解是理解个性化肠道微生物群反应的关键：双歧杆菌在肠道淀粉颗粒上的作用。

IF 2.1

Bioscience of microbiota, food and health Pub Date : 2026-01-01 Epub Date: 2026-03-04 DOI: 10.12938/bmfh.2025-065

Yusuke Nagara

{"title":"Microbiogeographic insights as keys to understanding personalized gut microbiota responses: the role of Bifidobacterium residing on intestinal starch granules.","authors":"Yusuke Nagara","doi":"10.12938/bmfh.2025-065","DOIUrl":"10.12938/bmfh.2025-065","url":null,"abstract":"There is growing optimism regarding the potential therapeutic and preventive benefits of regulating intestinal microbiota for various diseases. Diet is one of the most straightforward and safest methods for modulating the intestinal microbiota; however, considerable individual differences have been observed in the microbiota response to dietary interventions. These individual differences pose substantial challenges in application, which are primarily attributed to variations in the commensal flora and bacterial competition for nutrients. Our previous research indicated that the microscopic localization of bacteria provides valuable insights into the mechanisms by which specific intestinal bacterial species acquire nutrients within a competitive gut environment. Furthermore, our analysis revealed that the combination of bifidobacterial species and the nutrient source found in the localization analysis determined individual differences in microbiota response. These findings suggest that bacterial colonization facilitates the efficient, preferential, and presumably exclusive utilization of solid nutrient sources in the human gut. Moreover, the impact of a single nutrient source on the gut and human body may vary depending on the presence or absence of the primary species colonizing that source. In this review, we examined the micrometer-scale localization of intestinal bacteria and individual variability in microbiota responses to diet, drawing upon the results of our previous studies.","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 3","pages":"163-172"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148371286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Bacillus subtilis isolated from the rumen contents of Mongolian pasture-grazing sheep. 蒙古放牧羊瘤胃内容物分离的枯草芽孢杆菌的鉴定。

IF 2.1

Bioscience of microbiota, food and health Pub Date : 2026-01-01 Epub Date: 2025-11-04 DOI: 10.12938/bmfh.2025-029

Tsogtbaatar Lkhagvajav, Purevtsogt Dorjgoo, Olzii-Uchral Davaasuren, Banzragch Sukhbat, Tumenjargal Bazarragchaa, Dugersuren Jamyan, Narantuya Ayushjav, Zolzaya Majigsuren, Yuan-Kun Lee, Jiro Nakayama, Jin Shan, Dacheng Liu, Demberel Shirchin

{"title":"Characterization of Bacillus subtilis isolated from the rumen contents of Mongolian pasture-grazing sheep.","authors":"Tsogtbaatar Lkhagvajav, Purevtsogt Dorjgoo, Olzii-Uchral Davaasuren, Banzragch Sukhbat, Tumenjargal Bazarragchaa, Dugersuren Jamyan, Narantuya Ayushjav, Zolzaya Majigsuren, Yuan-Kun Lee, Jiro Nakayama, Jin Shan, Dacheng Liu, Demberel Shirchin","doi":"10.12938/bmfh.2025-029","DOIUrl":"10.12938/bmfh.2025-029","url":null,"abstract":"The aim of this study was to isolate and select biologically active local strains of Bacillus subtilis from the rumen contents of Mongolian pasture-grazing sheep and to evaluate their potential probiotic characteristics for application in ruminant health. Samples of rumen contents were collected from 15 sheep grazing in Bayan-Unjuul soum, Tuv province, Mongolia, and 62 primary isolates were obtained through culturing on selective solid and liquid media. The primary isolates were evaluated based on their morphological, biochemical, and physiological characteristics, enzymatic activities (amylase, protease, catalase), ability to produce acetoin, milk-coagulating ability, antagonistic activity, antibiotic susceptibility, and growth conditions. Phenotypic and genotypic analyses confirmed that these isolates belonged to the Bacillus subtilis species. Among the isolates, Bacillus subtilis c2-7c demonstrated superior probiotic properties for animal use, including strong enzymatic activity, antagonistic effects, and favorable antibiotic resistance profiles. The results show that the Bacillus subtilis c2-7c strain isolated and selected in this study has the most active probiotic characteristics, making it a promising candidate for applications in animal husbandry and veterinary medicine.","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 2","pages":"115-126"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13083183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147725054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Longitudinal changes in the gut microbiota with the progression of obesity-associated type 2 diabetes in KK-Ay mice. KK-Ay小鼠肠道微生物群纵向变化与肥胖相关的2型糖尿病进展

IF 2.1

Bioscience of microbiota, food and health Pub Date : 2026-01-01 Epub Date: 2026-01-20 DOI: 10.12938/bmfh.2025-104

Moeka Yasui, Hinata Takahashi, Fumina Ohsaka, Kei Sonoyama

引用次数: 0

Research on the combination of Polygonatum sibiricum and Poria cocos for preventing constipation due to pi deficiency. 黄精与茯苓配伍预防肾虚便秘的研究。

IF 2.1

Bioscience of microbiota, food and health Pub Date : 2026-01-01 Epub Date: 2025-11-25 DOI: 10.12938/bmfh.2025-045

Xianqing Sun, Fan Jiang, Zhoujin Tan, Nenqun Xiao

{"title":"Research on the combination of Polygonatum sibiricum and Poria cocos for preventing constipation due to pi deficiency.","authors":"Xianqing Sun, Fan Jiang, Zhoujin Tan, Nenqun Xiao","doi":"10.12938/bmfh.2025-045","DOIUrl":"10.12938/bmfh.2025-045","url":null,"abstract":"This study investigated the preventive effects of combination of Polygonatum sibiricum and Poria cocos on constipation due to pi deficiency and aimed to explore a potential early intervention strategy. The administered groups were gavaged with medicinal liquids containing different ratios of Polygonatum sibiricum to Poria cocos (A, 3:1; B, 1:1; and C, 1:3) for 14 days. Subsequently, a mouse model of constipation due to pi deficiency was established through a combination of Folium Sennae decoction gavage, diet regulation, water restriction, and abnormal hunger and satiety. The normal group was fed normally for 29 days. After 14 days of normal feeding, the model group mice were modeled according to the above modeling method. The study compared fecal characteristics, water content, serum D-xylose levels, intestinal microbial counts, and enzyme activities among the different groups. The results showed that mice with constipation due to pi deficiency exhibited altered body weights and behavior, along with altered fecal features and reduced fecal water content. Dysbiosis of the intestinal microbiota and disturbances in enzyme activities were observed. The mice in the administered groups showed less fluctuation in body weight and improved behavior. Fecal traits were improved, and the water content of feces was increased. Notably, ratio B (1:1) demonstrated the most significant effects, effectively restoring the intestinal flora balance and alleviating enzyme activity disorders. These findings suggest that the combination of Polygonatum sibiricum and Poria cocos at a 1:1 ratio exerts the most beneficial preventive effects on constipation due to pi deficiency by improving intestinal health and microbial homeostasis.","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 2","pages":"127-138"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13084061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147724973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Milk fat globule membrane ameliorates depressive-like behaviors in chronic unpredictable mild stress rats by modulating the microbiota-gut-brain axis. 乳脂球膜通过调节微生物-肠-脑轴改善慢性不可预测轻度应激大鼠的抑郁样行为。

IF 2.9

Bioscience of microbiota, food and health Pub Date : 2026-01-01 Epub Date: 2025-10-03 DOI: 10.12938/bmfh.2025-031

Li Huang, Yunqin Zheng, Qianfeng Liu, Yiran Feng, Zewei Ma, Xinrui Zhao, Xinru Wei, Xinyue Yu, Xia Lv, Jie Lv, Liang Li, Huan Liu, Xiaolei Ze, Meilin Zhang

{"title":"Milk fat globule membrane ameliorates depressive-like behaviors in chronic unpredictable mild stress rats by modulating the microbiota-gut-brain axis.","authors":"Li Huang, Yunqin Zheng, Qianfeng Liu, Yiran Feng, Zewei Ma, Xinrui Zhao, Xinru Wei, Xinyue Yu, Xia Lv, Jie Lv, Liang Li, Huan Liu, Xiaolei Ze, Meilin Zhang","doi":"10.12938/bmfh.2025-031","DOIUrl":"10.12938/bmfh.2025-031","url":null,"abstract":"Depression is one of the common psychiatric disorders, and it has been reported that the imbalance in the microbiota-gut-brain (MGB) axis contributes to the pathogenesis of depression. Milk fat globule membrane (MFGM) can impact the gut-brain axis by regulating the intestinal flora and metabolite production. The aim of this study was to investigate whether MFGM could ameliorate depressive-like behaviors induced by chronic unpredictable mild stress (CUMS) and further elucidate the potential mechanism through a fecal microbiota transplantation (FMT) experiment. Male Sprague-Dawley rats were provided with an MFGM diet for 5 weeks after the induction with CUMS. Depressive-like behaviors were assessed, and the levels of neurotransmitters, neuroendocrine hormones, microbiota, short-chain fatty acids (SCFAs), and tight junction proteins, including occludin and zonula occludens-1 (ZO-1), were measured. It was revealed that MFGM could alleviate the depressive-like behaviors in CUMS rats. MFGM up-regulated the expression of occludin and ZO-1 and ameliorated intestine pathological changes in CUMS rats. Moreover, MFGM increased the levels of 5-hydroxytryptamine (5-HT), dopamine (DA), and norepinephrine and decreased the levels of neuroendocrine hormones in CUMS rats. Furthermore, it was confirmed that the concentrations of SCFAs, DA, 5-HT, and tight junction proteins significantly increased in the recipient rats that were inoculated with the fecal microbiota from the rats after MFGM treatment. These findings demonstrated that MFGM could alleviate depressive-like behaviors in CUMS rats and was possibly associated with modulation of the gut microbiota and up-regulation of SCFAs and monoamine neurotransmitters.","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 1","pages":"66-78"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12765539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145907250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fermentation of soymilk with Lacticaseibacillus paracasei strain Shirota reduces soluble Gly m 4 levels and allergic reactions associated with soybean-related pollen-food allergy syndrome. 用副干酪乳杆菌Shirota菌株发酵豆浆可降低可溶性Gly - 4水平和与大豆相关的花粉-食物过敏综合征相关的过敏反应。

IF 2.9

Bioscience of microbiota, food and health Pub Date : 2026-01-01 Epub Date: 2025-10-03 DOI: 10.12938/bmfh.2025-025

Takahiro Shiba, Toshihide Kobayashi, Emi Iwata, Kazuhiko Yoshizawa, Atsuko Adachi, Tatsuya Moriyama

{"title":"Fermentation of soymilk with Lacticaseibacillus paracasei strain Shirota reduces soluble Gly m 4 levels and allergic reactions associated with soybean-related pollen-food allergy syndrome.","authors":"Takahiro Shiba, Toshihide Kobayashi, Emi Iwata, Kazuhiko Yoshizawa, Atsuko Adachi, Tatsuya Moriyama","doi":"10.12938/bmfh.2025-025","DOIUrl":"10.12938/bmfh.2025-025","url":null,"abstract":"Soybean-related pollen-food allergy syndrome (PFAS) is a food allergy triggered by birch or alder pollen-specific immunoglobulin E, which cross-reacts with soybean proteins homologous to pollen proteins. Although soybean-related PFAS generally causes mild symptoms, some individuals develop severe symptoms, including anaphylaxis, especially after consuming soymilk. This study attempted to reduce the risk of allergy by fermenting soymilk with Lacticaseibacillus paracasei strain Shirota (YIT 9029, LcS). The levels of Gly m 4, which causes soybean-related PFAS, in soymilk and fermented soymilk were analyzed using enzyme-linked immunosorbent assay (ELISA) and western blotting. Reactions to soymilk and fermented soymilk were evaluated in patients with soybean-related PFAS using skin prick tests to compare allergic risks. Gly m 4 levels in the soluble fraction of fermented soymilk were significantly lower than those in soymilk. The reduction in Gly m 4 levels was associated with alterations in the fermentation process, including pH reduction. Additionally, compared with soymilk, fermented soymilk reduced skin prick test reactions in patients with soybean-related PFAS. In conclusion, fermenting soymilk with LcS reduced soluble Gly m 4 levels and alleviated allergic reactions associated with soybean-related PFAS. This study demonstrates the potential of fermented soymilk as a safe and effective alternative for individuals with soybean-related PFAS.","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 1","pages":"58-65"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12765538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145907253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological study on the effects of gut microbiota and nutrients on breath hydrogen and methane concentrations. 肠道菌群和营养物质对呼吸氢和甲烷浓度影响的流行病学研究。

IF 2.9

Bioscience of microbiota, food and health Pub Date : 2026-01-01 Epub Date: 2025-08-21 DOI: 10.12938/bmfh.2025-044

Satoshi Sato, Daisuke Chinda, Shigeyuki Nakaji, Masakazu Tobinai, Nao Ishidoya, Keisuke Furusawa, Kaede Miyashiro, Kenta Yoshida, Chikara Iino, Kaori Sawada, Tatsuya Mikami, Hirotake Sakuraba

{"title":"Epidemiological study on the effects of gut microbiota and nutrients on breath hydrogen and methane concentrations.","authors":"Satoshi Sato, Daisuke Chinda, Shigeyuki Nakaji, Masakazu Tobinai, Nao Ishidoya, Keisuke Furusawa, Kaede Miyashiro, Kenta Yoshida, Chikara Iino, Kaori Sawada, Tatsuya Mikami, Hirotake Sakuraba","doi":"10.12938/bmfh.2025-044","DOIUrl":"10.12938/bmfh.2025-044","url":null,"abstract":"Breath hydrogen concentration measurement is a valuable tool for assessing the intestinal environment; however, few epidemiological studies have investigated the relationship between exhaled hydrogen and gut microbiota in healthy subjects. This study aimed to epidemiologically elucidate the relationships between exhaled hydrogen, gut microbiota, and nutrient intake in healthy residents of the Iwaki area of Hirosaki City, Aomori Prefecture, including those who exhaled methane. We categorized participants into low- and high-exhaled hydrogen groups based on the median exhaled hydrogen concentration of 6.13 ppm and matched background factors by propensity score matching for age, body mass index, and defecation habits. In the high exhaled hydrogen group, intestinal butyrate-producing bacteria such as Faecalibacterium, Anaerostipes, and Roseburia increased, and Bacteroides strains decreased. In addition, the group with high exhaled hydrogen concentrations had a high dietary fiber intake, and positive correlation was observed between dietary fiber intake and butyrate-producing bacteria. This trend was particularly pronounced for soluble dietary fiber. The exhaled methane concentration decreased in the higher exhaled hydrogen concentration group, and intestinal Methanobrevibacter was positively correlated with the exhaled methane concentration, although in extremely small amounts. No significant relationship was found between each nutrient intake and Methanobrevibacter strain. Measurement of the exhaled hydrogen concentration is useful for assessing the intestinal environment associated with nutritional intake. However, methane gas production was not changed by dietary intake, suggesting that intervention with prebiotics may be necessary.","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 1","pages":"9-18"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12765536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145907260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Tongxie Yaofang formula on the gut microbiota of diarrhea model mice: a combined strategy of network pharmacology, molecular docking, and 16S rRNA gene sequencing. 通泻药方对腹泻模型小鼠肠道菌群的影响：网络药理学、分子对接、16S rRNA基因测序联合策略

IF 2.1

Bioscience of microbiota, food and health Pub Date : 2026-01-01 Epub Date: 2026-05-12 DOI: 10.12938/bmfh.2026-027

Qin Liu, Huiying Liu, Huiyi Peng, Zhen Yuan, Lan Shu, Zhoujin Tan

{"title":"Effect of Tongxie Yaofang formula on the gut microbiota of diarrhea model mice: a combined strategy of network pharmacology, molecular docking, and 16S rRNA gene sequencing.","authors":"Qin Liu, Huiying Liu, Huiyi Peng, Zhen Yuan, Lan Shu, Zhoujin Tan","doi":"10.12938/bmfh.2026-027","DOIUrl":"10.12938/bmfh.2026-027","url":null,"abstract":"Tongxie Yaofang (TXYF) is a classic formula for liver stagnation and spleen deficiency diarrhea, but its mechanisms involving the gut microbiota remain unclear. This study aimed to elucidate the mechanisms TXYF by integrating network pharmacology, molecular docking, and 16S rRNA gene sequencing. We employed network pharmacology and molecular docking to identify bioactive compounds, targets, and their associations with gut microbiota metabolites. Sequencing of the 16S rRNA gene revealed changes in the gut microbiota in a diarrheal mouse model after TXYF intervention. Thirty-nine active compounds (525 targets) were identified. Intersection analysis yielded 44 shared targets among TXYF, diarrhea, and gut microbiota metabolites. Molecular docking demonstrated favorable binding of the selected active compounds and representative gut microbiota-derived metabolites with the core targets GSK3B, TP53, and MAPK3. Sequencing of the 16S rRNA gene also showed that TXYF significantly modulated gut microbiota structure, notably altering abundances of Corynebacterium stationis, Proteus vulgaris, and Proteus mirabilis. TXYF treats diarrhea by modulating gut microbiota composition and maintaining microbial homeostasis via a multi-component, multi-target mechanism, supporting its potential as a microbiota-targeted therapy.","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 3","pages":"225-236"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148371317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host genotype is associated with selective colonization of human fecal microbiota in NCG and SGM3 mice: implications for microbiota-based therapies. 宿主基因型与人类粪便微生物群在NCG和SGM3小鼠中的选择性定植有关：对微生物群治疗的影响

IF 2.1

Bioscience of microbiota, food and health Pub Date : 2026-01-01 Epub Date: 2026-03-09 DOI: 10.12938/bmfh.2025-102

Kexin Yang, Guang Peng, Xinyao Zhang, Dingzhen Yang, Yizhou Wang, Renjin Chen

{"title":"Host genotype is associated with selective colonization of human fecal microbiota in NCG and SGM3 mice: implications for microbiota-based therapies.","authors":"Kexin Yang, Guang Peng, Xinyao Zhang, Dingzhen Yang, Yizhou Wang, Renjin Chen","doi":"10.12938/bmfh.2025-102","DOIUrl":"10.12938/bmfh.2025-102","url":null,"abstract":"Humanized mouse models are widely used to investigate host-microbiota interactions, yet the extent to which host background contributes to engraftment fidelity remains incompletely defined. In this study, we transplanted fecal microbiota from healthy human donors into NCG and SGM3 mice and characterized engraftment using 16S rRNA sequencing. Both models exhibited reduced diversity relative to donors, but their colonization trajectories diverged. NCG recipients appeared closer to donors in β-diversity space, a pattern largely associated with the expansion of a limited set of opportunistic Proteobacteria such as Escherichia-Shigella and Citrobacter. In contrast, SGM3 mice displayed modestly higher α-diversity and retained a broader set of donor-associated genera, with selective enrichment of Bacillus, yet exhibited greater predicted functional divergence, with reductions in pathways related to ABC transport and carbohydrate metabolism. Several strictly anaerobic commensals, including Faecalibacterium, failed to colonize in either genotype. Collectively, these findings suggest that host genotype is associated with selective colonization of human fecal microbiota and support the utility of integrating compositional and functional criteria when selecting experimental models for translational microbiome research.","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":"45 3","pages":"188-196"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148371349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0