Adriyan Pramono, Martha Ardiaria, Edward Kurnia Setiawan Limijadi, Etika Ratna Noer, Endang Sri Lestari, Ferbian Milas Siswanto
{"title":"Intermittent fasting modulates human gut microbiota diversity in a phenotype-dependent manner: a systematic review.","authors":"Adriyan Pramono, Martha Ardiaria, Edward Kurnia Setiawan Limijadi, Etika Ratna Noer, Endang Sri Lestari, Ferbian Milas Siswanto","doi":"10.12938/bmfh.2023-111","DOIUrl":"10.12938/bmfh.2023-111","url":null,"abstract":"<p><p>Cumulative evidence suggests that intermittent fasting (IF) has beneficial effects on human metabolic health. It has been indicated that its impact on the gut microbiota may mediate these beneficial effects. As a result, we hypothesized that IF may impact the human gut microbiota. A systematic review was carried out according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) protocol using the PubMed, Scopus, and CINAHL databases. We registered our systematic review protocol in PROSPERO under registration number CRD42021270050. Human intervention studies published until April 30, 2023, were included. The quality of the included studies was assessed using National Institutes of Health (NIH) quality assessment study tools for intervention studies. The search in the database returned 166 studies, of which 13 matched all criteria for the final qualitative analysis. The body of evidence suggests that IF modulates human gut microbiota alpha and beta diversity in lean (relatively healthy) and relatively healthy overweight/obese individuals but not in individuals with metabolic syndrome. Furthermore, IF also alters human gut microbiota composition in all phenotypes. Of interest, the gut microbiota taxa or microbial metabolites after an IF intervention are associated with metabolic markers. According to this review, IF influences the diversity and taxonomic levels of the human gut microbiota. Individual metabolic phenotypes may alter the effect of IF on the diversity and taxonomic levels of the gut microbiota.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11220331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141536226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of prebiotic properties of rice bran extract.","authors":"Thornthan Sawangwan, Daleena Kajadman, Ratchanon Kulchananimit","doi":"10.12938/bmfh.2023-090","DOIUrl":"10.12938/bmfh.2023-090","url":null,"abstract":"<p><p>This research investigated and compared the prebiotic properties of a rice bran extract obtained through commercial xylanase extraction in comparison with water extraction. Prebiotic properties were evaluated by probiotic growth stimulation (<i>Lacticaseibacillus casei</i> and <i>Lactiplantibacillus plantarum</i>) and gastrointestinal pathogen inhibition (<i>Bacillus cereus</i> and <i>Escherichia coli</i>). The rice bran extract obtained with xylanase (RB1) displayed significantly higher total polysaccharide and total reducing sugar contents than those obtained with water (RB2; p<0.05). After extraction for 30 min, RB1 exhibited the highest total polysaccharide and total reducing sugar contents. HPLC (high performance liquid chromatography) analysis revealed that RB1 primarily contained xylose, while RB2 contained less glucose and lacked other sugar derivatives. RB1 proved effective in stimulating the growth of <i>L. casei</i> and <i>L. plantarum</i>, surpassing even inulin (a commercial prebiotic). Furthermore, it demonstrated a high potential for inhibiting the growth of pathogenic <i>B. cereus</i> and <i>E. coli,</i> comparable to inulin. In contrast, RB2 exhibited lower inhibitory capacity against <i>B. cereus</i> and <i>E. coli</i>.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11220324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141536351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of multi-strain bifidobacteria supplementation on intestinal microbiota development in low birth weight neonates: a randomized controlled trial.","authors":"Kenichi Takeshita, Haruka Takei, Saori Tanaka, Haruka Hishiki, Yuta Iijima, Hitoshi Ogata, Kensuke Fujishiro, Takahiro Tominaga, Yuki Konno, Yukiko Iwase, Mamiko Endo, Naruhiko Ishiwada, Yoshiteru Osone, Ryo Takemura, Hiromichi Hamada, Naoki Shimojo","doi":"10.12938/bmfh.2023-093","DOIUrl":"10.12938/bmfh.2023-093","url":null,"abstract":"<p><p>Single-strain <i>Bifidobacterium</i> species are commonly used as probiotics with low birth weight neonates. However, the effectiveness and safety of multi-strain <i>Bifidobacterium</i> supplementation are not well known. Thirty-six neonates weighing less than 2,000 g (558-1,943 g) at birth and admitted to a neonatal intensive care unit were randomly assigned to receive a single strain or triple strains of <i>Bifidobacterium</i> with lactulose enterally for 4 weeks from birth. The relative abundances of <i>Staphylococcus</i> and <i>Bifidobacterium</i> in the fecal microbiota at weeks 1, 2, and 4 were investigated. Based on the study results, no significant difference was detected between the two groups in the abundance of <i>Staphylococcus</i>; however, the triple-strain group had significantly high abundances of <i>Bifidobacterium</i> at weeks 2 and 4. The fecal microbiota in the triple-strain group had significantly lower alpha diversity (<i>Bifidobacterium</i>-enriching) after week 4 and was different from that in the single-strain group, which showed a higher abundance of <i>Clostridium.</i> No severe adverse events occurred in either group during the study period. Although no significant difference was detected between single- and multi-strain bifidobacteria supplementation in the colonization of <i>Staphylococcus</i> in the fecal microbiota of the neonates, multi-strain bifidobacteria supplementation contributed toward early enrichment of the microbiota with bifidobacteria and suppression of other pathogenic bacteria, such as <i>Clostridium</i> spp.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11444860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of applying <i>Lactiplantibacillus plantarum</i> subsp. <i>plantarum</i> N793 to the scalps of men and women with thinning hair: a randomized, double-blind, placebo-controlled, parallel-group study.","authors":"Ayaka Mori-Ichioka, Yosuke Sunada, Takashi Koikeda, Hideo Matsuda, Shinji Matsuo","doi":"10.12938/bmfh.2023-056","DOIUrl":"10.12938/bmfh.2023-056","url":null,"abstract":"<p><p><i>Lactiplantibacillus plantarum</i> subsp. <i>plantarum</i> N793 (N793) is a lactic acid bacterium (LAB) isolated from corn. We previously showed that N793 increases the level of keratinocyte growth factor, which is required for hair growth, in the culture supernatant of human follicle dermal papilla cells. Additionally, an open-label, single-arm study reported that applying a lotion containing N793 to the scalp for 24 weeks improved hair density in men and women with thinning hair. The present study was a double-blind, placebo-controlled, parallel-group study aimed at verifying the efficacy of N793 for thinning hair. A lotion containing N793, and a control lotion (placebo) were applied once daily for 24 weeks to 104 healthy Japanese men and women. Analysis of all participants revealed no difference in hair density between the N793 and placebo groups. However, an additional analysis limited to participants with relatively mild progression of thinning hair showed a significantly better hair density in the N793 group than in the placebo group. These findings suggest that topical application of N793 improves thinning hair in men and women when the condition's progression is relatively mild.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11220327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141536213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Lot-to-lot variation in the microbiota during the brewing process of <i>kimoto</i>-type Japanese rice wine.","authors":"Momoka Yamane, Shuntaro Sakai, Miho Hirai, Mizuki Takayama, Kohya Sasayama, Kazutoshi Douchi, Shinji Kawabata, Shinya Ikeda, Masayuki Sugawara","doi":"10.12938/bmfh.2023-092","DOIUrl":"10.12938/bmfh.2023-092","url":null,"abstract":"<p><p><i>Kimoto</i>-type Japanese rice wine (<i>sake</i>) has a wide variety of flavors, as the predominant microbes, including lactic acid bacteria (LAB) and nitrate-reducing bacteria, that spontaneously proliferate in the fermentation starter vary depending on the brewery. In this study, we traced the microbiota in four lots of starters manufactured in a newly established brewery and evaluated the lot-to-lot variation and characteristics of the microbiota in the brewery. The results of a 16S ribosomal RNA amplicon analysis showed that the starters brewed in the second brewing year had a more diverse microbiota than those in the first brewing year. Among the LAB predominated at the middle production stage, lactococci, including <i>Leuconostoc</i> spp., were detected in all the lots, while lactobacilli predominated for the first time in the second year. These results suggest that repeated brewing increased microbial diversity and altered the microbial transition pattern in the <i>kimoto</i>-style fermentation starters. Phylogenetic analyses for the LAB isolates from each starter identified <i>Leuconostoc suionicum, Leuconostoc citreum</i>, and <i>Leuconostoc mesenteroides</i> as predominant lactococci as well as a unique lactobacillus in place of <i>Latilactobacillus sakei</i>. We also found that a rice <i>koji</i>-derived <i>Staphylococcus gallinarum</i> with nitrate-reducing activity was generally predominant during the early production stage, suggesting that there was a case in which staphylococci played a role in nitrite production in the starters. These findings are expected to contribute to the understanding of the diversity of microbiota in <i>kimoto</i>-type <i>sake</i> brewing and enable control of the microbiota for consistent <i>sake</i> quality.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11220333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141536227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yang Ping, Jianing Liu, Huilin Wang, Yan Wang, Hongbin Qiu, Yu Zhang
{"title":"Research progress in the treatment of an immune system disease-type 1 diabetes-by regulating the intestinal flora with Chinese medicine and food homologous drugs.","authors":"Yang Ping, Jianing Liu, Huilin Wang, Yan Wang, Hongbin Qiu, Yu Zhang","doi":"10.12938/bmfh.2023-068","DOIUrl":"10.12938/bmfh.2023-068","url":null,"abstract":"<p><p>Type 1 diabetes (T1D) is a specific autoimmune disease related to genetic and autoimmune factors. Recent studies have found that the intestinal flora is one of the important environmental factors in the development of T1D. The gut microbiota is the largest microbiota in the human body and has a significant impact on material and energy metabolism. Related studies have found that the intestinal floras of T1D patients are unbalanced. Compared with normal patients, the abundance of beneficial bacteria is reduced, and various pathogenic bacteria are significantly increased, affecting the occurrence and development of diabetes. Medicinal and food homologous traditional Chinese medicine (TCM) has a multicomponent, multitarget, and biphasic regulatory effect. Its chemical composition can increase the abundance of beneficial bacteria, improve the diversity of the intestinal flora, reduce blood sugar, and achieve the purpose of preventing and treating T1D by regulating the intestinal flora and its metabolites. Therefore, based on a review of T1D, intestinal flora, and TCM derived from medicine and food, this review describes the relationship between T1D and the intestinal flora, as well as the research progress of TCM interventions for T1D through regulation of the intestinal flora. Medicine and food homologous TCM has certain advantages in treating diabetes and regulating the intestinal flora. It can be seen that there is still great research space and broad development prospects for the treatment of diabetes by regulating the intestinal flora with drug and food homologous TCM.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11220337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141536229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunomodulatory effects of <i>Ulva</i>-derived polysaccharides, oligosaccharides, and residues in a murine model of delayed-type hypersensitivity.","authors":"Jing-Yi Ou, Fang-Ling Liu, Chien-Li Chen, Ming-Chih Fang, Chung-Hsiung Huang","doi":"10.12938/bmfh.2023-065","DOIUrl":"10.12938/bmfh.2023-065","url":null,"abstract":"<p><p><i>Ulva</i>, an edible green alga, contains sulfated polysaccharides and oligosaccharides that possess immunomodulatory and anti-inflammatory properties. The objective of this study was to investigate the anti-allergic effects of <i>Ulva</i>-derived samples of polysaccharides (UP), oligosaccharides (UO), and residues (UR) on delayed-type hypersensitivity (DTH) in mice. Oral treatment of mice with UP, UO, and UR (250 mg/kg body weight) daily noticeably improved the DTH reaction as evidenced by attenuation of footpad swelling and cell infiltration at the allergen-challenge site. Although the <i>Ulva</i> samples had limited impacts on the production of serum total IgG, decreased concentrations of allergen-specific IgG and IgG<sub>2a</sub> and an increased concentration of IgG<sub>1</sub> were observed in the treated mice. Moreover, treatment with them suppressed allergen-induced IFN-γ and TNF-α secretion and elevated IL-4 secretion. However, none of the <i>Ulva</i> sample treatments could modulate the production of IL-10. Concordantly, the <i>in situ</i> data reveal that the <i>Ulva</i> sample treatments suppressed IFN-γ and TNF-α expression at the allergen-injection site. These findings collectively suggest the potential of UP, UO, and UR as functional food candidates for the management of delayed-type hypersensitivity.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10981946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140338053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hendra Zufry, Putri Oktaviani Zulfa, Timotius Ivan Hariyanto
{"title":"The gut microbiota and its role in Graves' Disease: a systematic review and meta-analysis.","authors":"Hendra Zufry, Putri Oktaviani Zulfa, Timotius Ivan Hariyanto","doi":"10.12938/bmfh.2024-020","DOIUrl":"10.12938/bmfh.2024-020","url":null,"abstract":"<p><p>Emerging research indicates the potential involvement of gut bacteria in the etiology of Graves' Disease (GD). However, the evidence regarding this matter is still conflicting. The primary objective of this investigation was to examine the correlation between gut microbiota and GD. A comprehensive search was conducted of the Cochrane Library, Scopus, Europe PMC, and Medline databases up until August 1, 2023, utilizing a combination of relevant keywords. This review incorporates literature that examined the composition of gut microbiota in patients with GD. We employed random-effect models to analyze the standardized mean difference (SMD) and present the outcomes together with their corresponding 95% confidence intervals (CIs). A total of ten studies were incorporated. The results of our meta-analysis indicated that patients with GD have a reduced alpha diversity of gut microbiota as evidence by a significant reduction of Chao1 (std. mean difference -0.58; 95% CI -0.90, -0.26, p=0.0004; <i>I<sup>2</sup></i> =61%), ACE (std. mean difference -0.64; 95% CI -1.09, -0.18, p=0.006; <i>I<sup>2</sup></i> =77%), and Shannon index (std. mean difference -0.71; 95% CI -1.25, -0.17, p=0.01; <i>I<sup>2</sup></i> =90%) when compared with healthy controls. At the phylum level, the abundance of Firmicutes was reduced in GD patients, while that of Bacteroidetes was increased. This study suggests a notable decrease in the richness and variety of gut microbiota among people diagnosed with GD in comparison with healthy controls.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11444861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shino Takeuchi, Mohammad Shaokat Ali, Yoshihiko Tanimoto, Eriko Kage-Nakadai
{"title":"<i>Lactococcus kimchii</i> extends lifespan and alleviates motility decline in <i>Caenorhabditis elegans</i> through <i>ins-20</i>, an insulin-like peptide gene.","authors":"Shino Takeuchi, Mohammad Shaokat Ali, Yoshihiko Tanimoto, Eriko Kage-Nakadai","doi":"10.12938/bmfh.2023-091","DOIUrl":"10.12938/bmfh.2023-091","url":null,"abstract":"<p><p><i>Lactococcus kimchii</i> is isolated from commercial kimchi, which is a traditional Korean fermented food. This study was conducted to evaluate the probiotic effects of <i>L. kimchii</i>. <i>Caenorhabditis elegans</i> was fed <i>L. kimchii</i>, and its longevity, motility, and gene expression were examined. When fed a 1:1 mixture of <i>Escherichia coli</i> OP50 and <i>L. kimchii</i> (OP+LK), <i>C. elegans</i> had a significantly longer lifespan and increased locomotion than when it was fed OP alone. There was no significant difference in brood size between the OP+LK and OP groups, suggesting that these effects occurred in a dietary restriction-independent manner. RNA sequencing and Gene Ontology analysis showed that the expression of <i>ins-20</i>, an insulin-like peptide and agonist of the insulin receptor, was significantly upregulated in the OP+LK group. The <i>ins-20</i> mutation annulled the effects of OP+LK on lifespan extension and motility. In addition, OP+LK failed to extend the lifespan of <i>C. elegans</i> deficient in <i>daf-2</i>, a receptor for the insulin-like signaling pathway. These results suggest that <i>L. kimchii</i> extends the lifespan and alleviates motility decline in <i>C. elegans</i> through the insulin signaling pathway, highlighting the potential of using <i>L. kimchii</i> as a beneficial bacterium for probiotics and postbiotics.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11220334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141536208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yutaka Makizaki, Mana Kishimoto, Yoshiki Tanaka, Hiroshi Ohno
{"title":"Activation of chloride channels and promotion of bowel movements by heat-killed <i>Bifidobacterium longum</i> CLA8013.","authors":"Yutaka Makizaki, Mana Kishimoto, Yoshiki Tanaka, Hiroshi Ohno","doi":"10.12938/bmfh.2023-084","DOIUrl":"10.12938/bmfh.2023-084","url":null,"abstract":"<p><p>Constipation is strongly associated with the deterioration of quality of life (QOL), and patients with constipation desire clear spontaneous defecation without the feeling of incomplete evacuation, rather than improved defecation frequency. The use of common osmotic or stimulant laxatives has not been shown to lead to a satisfactory improvement of bowel movements. In addition, softening of stools by increasing their water content has been reported to increase the frequency of spontaneous defecation and improve hard stools, straining during defecation, and abdominal symptoms, such as abdominal bloating, thereby leading to improvement of QOL deterioration caused by constipation. Thus, the present study screened bacterial strains <i>in vitro</i> using intestinal epithelial T84 cells, aiming to identify one that activates chloride channels involved in water secretion into the intestinal tract. As a result, the conditioned medium of <i>Bifidobacterium longum</i> CLA8013 was found to induce ion transport. Also, this effect was suppressed by cystic fibrosis transmembrane conductance regulator (CFTR) (inh)-172, a CFTR chloride channel inhibitor. Furthermore, both live and heat-killed CLA8013 similarly induced ion transport, suggesting that bacterial cell components are responsible for the effect. In addition, the administration of heat-killed CLA8013 to loperamide-induced constipation rats resulted in an increase in fecal water content and promoted defecation. These results suggest that the active components in CLA8013 act on CFTR chloride channels in the intestinal tract, promote water secretion into the intestinal tract, and soften stools, thereby promoting bowel movements.</p>","PeriodicalId":93908,"journal":{"name":"Bioscience of microbiota, food and health","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11220339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141536209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}