Correlation between differences in the intestinal flora structure and Chinese medicine evidence in patients with Wilson disease-related liver fibrosis analyzed via high-throughput sequencing technology.

IF 2.5 Q3 MICROBIOLOGY
Bioscience of microbiota, food and health Pub Date : 2025-01-01 Epub Date: 2024-12-11 DOI:10.12938/bmfh.2024-081
Yue Pu, Xinxiang Zhang, Juan Zhang, Daojun Xie, Han Wang, Hong Chen, Ying Ma, Nian Peng, Rui Li, Hao Ye
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Abstract

To observe the composition and abundance of the intestinal flora in patients with Wilson disease (WD)-related liver fibrosis and analyze the correlation between the composition of intestinal flora of patients and the evolution of evidence from Chinese medicine, we selected 237 patients with WD-related liver fibrosis and 30 healthy volunteers from the Brain Disease Center of Anhui Provincial Hospital of Chinese Medicine. The patients with WD-related liver fibrosis were divided into 5 groups according to traditional Chinese medicine (TCM) evidence (dampness-heat syndrome, group A; intermingled phlegm and blood stasis syndrome, group B; liver wind stirring up internally syndrome, group C; yin deficiency of the liver and kidney syndrome, group D; and yang deficiency of the spleen and kidney syndrome, group E) and a group healthy volunteers (group F), which served as the control. Stool samples were obtained from the patients in the 6 groups. The 16S rRNA sequencing technique was used to analyze the intestinal flora of the different TCM evidence groups of WD patients and the healthy control group and subjected to a statistical analysis. The intestinal flora abundance was significantly lower in patients with WD-related liver fibrosis than in healthy controls, and the decrease in strain content was more significant in patients with deficiency evidence in groups D and E. In terms of the structure of the phylum-level flora, the Firmicutes phylum was still the dominant phylum, but the contents of the evidence-type groups all decreased, with the most obvious decreases in groups D and E. The results for the Actinobacteria phylum were similar, whereas the opposite was true for the Proteobacteria phylum. The section-level and genus-level results corresponded to the gate level. The intestinal flora of the WD-related liver fibrosis patients and healthy controls differed in terms of abundance and intestinal flora structure, and there were also differences between different Chinese medicine certificates.

利用高通量测序技术分析肝豆状核变性相关肝纤维化患者肠道菌群结构差异与中医证据的相关性
为了观察Wilson病(WD)相关性肝纤维化患者肠道菌群的组成和丰度,并分析患者肠道菌群组成与中医证据演变的相关性,我们选择了安徽省中医院脑病中心237例WD相关性肝纤维化患者和30名健康志愿者。将wd相关性肝纤维化患者根据中医证候分为5组(A组湿热证;痰瘀混证B组;肝风内搅证,C组;肝肾阴虚证,D组;E组为脾虚肾阳虚证,F组为对照组。采集6组患者的粪便标本。采用16S rRNA测序技术对WD患者不同中医证据组和健康对照组的肠道菌群进行分析,并进行统计学分析。wd相关性肝纤维化患者肠道菌群丰度显著低于健康对照组,且D组和e组证据不足患者菌群含量下降更为显著。从门级菌群结构上看,厚壁菌门仍为优势门,但证据型组菌群含量均下降。放线菌门的结果与放线菌门相似,而变形菌门的结果则相反。切片水平和属水平的结果与门水平相对应。wd相关性肝纤维化患者与健康对照组肠道菌群在丰度和肠道菌群结构上存在差异,不同中医证之间也存在差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
6.20
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