Biomedical and environmental sciences : BES最新文献_第8页

Barley Protein LFBEP-C1 from Lactiplantibacillus plantarum dy-1 Fermented Barley Extracts by Inhibiting Lipid Accumulation in a Caenorhabditis elegans Model. 植物乳杆菌 dy-1 发酵大麦提取物中的大麦蛋白 LFBEP-C1 在秀丽隐杆线虫模型中抑制脂质积累

Biomedical and environmental sciences : BES Pub Date : 2024-04-20 DOI: 10.3967/bes2024.042

Jia Yan Zhang, Meng Ting Liu, Yu Hao Liu, Huan Deng, Juan Bai, Jian Hua Xie, Xiang Xiao

{"title":"Barley Protein LFBEP-C1 from Lactiplantibacillus plantarum dy-1 Fermented Barley Extracts by Inhibiting Lipid Accumulation in a Caenorhabditis elegans Model.","authors":"Jia Yan Zhang, Meng Ting Liu, Yu Hao Liu, Huan Deng, Juan Bai, Jian Hua Xie, Xiang Xiao","doi":"10.3967/bes2024.042","DOIUrl":"10.3967/bes2024.042","url":null,"abstract":"Objective: This study aimed to investigate the lipid-lowering activity of LFBEP-C1 in high glucose-fed Caenorhabditis elegans (C. elegans).Methods: In this study, the fermented barley protein LFBEP-C1 was prepared and tested for its potential anti-obesity effects on C. elegans. The worms were fed Escherichia coli OP50 ( E. coli OP50), glucose, and different concentrations of LFBEP-C1. Body size, lifespan, movement, triglyceride content, and gene expression were analyzed. The results were analyzed using ANOVA and Tukey's multiple comparison test.Results: Compared with the model group, the head-swing frequency of C. elegans in the group of LFBEP-C1 at 20 μg/mL increased by 33.88%, and the body-bending frequency increased by 27.09%. This indicated that LFBEP-C1 improved the locomotive ability of C. elegans. The average lifespan of C. elegans reached 13.55 days, and the body length and width of the C. elegans decreased after LFBEP-C1 intake. Additionally, LFBEP-C1 reduced the content of lipid accumulation and triglyceride levels. The expression levels of sbp-1, daf-2, and mdt-15 significantly decreased, while those of daf-16, tph-1, mod-1, and ser-4 significantly increased after LFBEP-C1 intake. Changes in these genes explain the signaling pathways that regulate lipid metabolism.Conclusion: LFBEP-C1 significantly reduced lipid deposition in C. elegans fed a high-glucose diet and alleviated the adverse effects of a high-glucose diet on the development, lifespan, and exercise behavior of C. elegans. In addition, LFBEP-C1 regulated lipid metabolism mainly by mediating the expression of genes in the sterol regulatory element-binding protein, insulin, and 5-hydroxytryptamine signaling pathways.","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140899977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Effect of Rhodopseudomonas palustris-Bioconverted Extract of Lycium barbarum on Rats with Lead Exposure. 枸杞生物转化提取物对铅暴露大鼠的治疗效果

Biomedical and environmental sciences : BES Pub Date : 2024-04-20 DOI: 10.3967/bes2024.050

Hong Yang, Yi Sun, Shu Tong Chai, Yan Hui Liang, Ya Ni Liu, Guan E Yang

引用次数: 0

Lecanemab Unveiled: Exploring Alzheimer's Treatment Advancements, Assessing Strengths, Limitations, and Its Therapeutic Landscape Position. Lecanemab 揭开面纱：探索阿尔茨海默氏症治疗进展，评估其优势、局限性及其治疗格局定位。

Biomedical and environmental sciences : BES Pub Date : 2024-04-20 DOI: 10.3967/bes2024.047

Victor Abiola Adepoju, Okechukwu Innocent Onyezue, Safayet Jamil, Olalekan John Okesanya, Eliseo Lucero-Prisno Iii Don

引用次数: 0

Prevalence, Characteristics and Treatment of Knee Osteoarthritis in Urban Residents of Beijing, China: A Community-Based Cross-Sectional Study. 中国北京城市居民膝骨关节炎的患病率、特征和治疗：一项基于社区的横断面研究。

Biomedical and environmental sciences : BES Pub Date : 2024-04-20 DOI: 10.3967/bes2024.045

Jun Zhou, Yan Yan, Ya Wei Dong, Xiao Yu Liu, Ping Wang, Jia Ming Lin, Peng Xuan Dong, Rui Quan, Rong Tian Wang, Bao Hong Mi, Wei Heng Chen

引用次数: 0

Establishment of a Multiplex Detection Method for Common Bacteria in Blood Based on Human Mannan-Binding Lectin Protein-Conjugated Magnetic Bead Enrichment Combined with Recombinase-Aided PCR Technology. 基于人甘露结合凝集素蛋白结合磁珠富集与重组酶辅助 PCR 技术的血液中常见细菌多重检测方法的建立

Biomedical and environmental sciences : BES Pub Date : 2024-04-20 DOI: 10.3967/bes2024.043

Zi Jin Zhao, Xiao Ping Chen, Shao Wei Hua, Feng Yu Li, Meng Zhao, Chen Hao Xing, Jie Wang, Feng Yu Tian, Rui Qing Zhang, Xiao Na Lyu, Zhi Qiang Han, Yu Xin Wang, Hong Yi Li, Xin Xin Shen, Xue Jun Ma, Yan Qing Tie

{"title":"Establishment of a Multiplex Detection Method for Common Bacteria in Blood Based on Human Mannan-Binding Lectin Protein-Conjugated Magnetic Bead Enrichment Combined with Recombinase-Aided PCR Technology.","authors":"Zi Jin Zhao, Xiao Ping Chen, Shao Wei Hua, Feng Yu Li, Meng Zhao, Chen Hao Xing, Jie Wang, Feng Yu Tian, Rui Qing Zhang, Xiao Na Lyu, Zhi Qiang Han, Yu Xin Wang, Hong Yi Li, Xin Xin Shen, Xue Jun Ma, Yan Qing Tie","doi":"10.3967/bes2024.043","DOIUrl":"10.3967/bes2024.043","url":null,"abstract":"Objective: Recombinase-aided polymerase chain reaction (RAP) is a sensitive, single-tube, two-stage nucleic acid amplification method. This study aimed to develop an assay that can be used for the early diagnosis of three types of bacteremia caused by Staphylococcus aureus (SA), Pseudomonas aeruginosa (PA), and Acinetobacter baumannii (AB) in the bloodstream based on recombinant human mannan-binding lectin protein (M1 protein)-conjugated magnetic bead (M1 bead) enrichment of pathogens combined with RAP.Methods: Recombinant plasmids were used to evaluate the assay sensitivity. Common blood influenza bacteria were used for the specific detection. Simulated and clinical plasma samples were enriched with M1 beads and then subjected to multiple recombinase-aided PCR (M-RAP) and quantitative PCR (qPCR) assays. Kappa analysis was used to evaluate the consistency between the two assays.Results: The M-RAP method had sensitivity rates of 1, 10, and 1 copies/μL for the detection of SA, PA, and AB plasmids, respectively, without cross-reaction to other bacterial species. The M-RAP assay obtained results for < 10 CFU/mL pathogens in the blood within 4 h, with higher sensitivity than qPCR. M-RAP and qPCR for SA, PA, and AB yielded Kappa values of 0.839, 0.815, and 0.856, respectively ( P < 0.05).Conclusion: An M-RAP assay for SA, PA, and AB in blood samples utilizing M1 bead enrichment has been developed and can be potentially used for the early detection of bacteremia.","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of the HIV/AIDS Epidemic among People Aged ≥ 50 Years in China during 2018-2021. 2018-2021年中国≥50岁人群艾滋病流行特征。

Biomedical and environmental sciences : BES Pub Date : 2024-04-20 DOI: 10.3967/bes2024.044

Yu Shan Hou, Yi Chen Jin, Chang Cai, Hou Lin Tang, Qian Qian Qin, Fan Lyu

{"title":"Characteristics of the HIV/AIDS Epidemic among People Aged ≥ 50 Years in China during 2018-2021.","authors":"Yu Shan Hou, Yi Chen Jin, Chang Cai, Hou Lin Tang, Qian Qian Qin, Fan Lyu","doi":"10.3967/bes2024.044","DOIUrl":"https://doi.org/10.3967/bes2024.044","url":null,"abstract":"Objective: This study aimed to determine the current epidemiological status of PLWHA aged ≥ 50 years in China from 2018 to 2021. It also aimed to recommend targeted interventions for the prevention and treatment of HIV/AIDS in elderly patients.Methods: Data on newly reported cases of PLWHA, aged ≥ 50 years in China from 2018 to 2021, were collected using the CRIMS. Trend tests and spatial analyses were also conducted.Results: Between 2018 and 2021, 237,724 HIV/AIDS cases were reported among patients aged ≥ 50 years in China. The main transmission route was heterosexual transmission (91.24%). Commercial heterosexual transmission (CHC) was the primary mode of transmission among males, while non-marital non-CHC ([NMNCHC]; 60.59%) was the prevalent route in women. The proportion of patients with CHC decreased over time ( Z = 67.716, P < 0.01), while that of patients with NMNCHC increased ( Z = 153.05, P < 0.01). The sex ratio varied among the different modes of infection, and it peaked at 17.65 for CHC. The spatial analysis indicated spatial clustering, and the high-high clustering areas were mainly distributed in the southwestern and central-southern provinces.Conclusion: In China, PLWHA, aged ≥ 50 years, were predominantly infected through heterosexual transmission. The primary modes of infection were CHC and NMNCHC. There were variations in the sex ratio among different age groups, infected through various sexual behaviors. HIV/AIDS cases exhibited spatial clustering. Based on these results, the expansion of HIV testing, treatment, and integrated behavioral interventions in high-risk populations is recommended to enhance disease detection in key regions.","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomics Study of Benzene Metabolite Hydroquinone Induced Hematotoxicity in K562 Cells. 苯代谢物对苯二酚诱导 K562 细胞血液毒性的蛋白质组学研究

Biomedical and environmental sciences : BES Pub Date : 2024-04-20 DOI: 10.3967/bes2024.039

Yi Shan Jin, Zong Chun Yi, Yu Jing Zhang, Long Rong, Chun Hong Yu

{"title":"Proteomics Study of Benzene Metabolite Hydroquinone Induced Hematotoxicity in K562 Cells.","authors":"Yi Shan Jin, Zong Chun Yi, Yu Jing Zhang, Long Rong, Chun Hong Yu","doi":"10.3967/bes2024.039","DOIUrl":"10.3967/bes2024.039","url":null,"abstract":"Objective: Hydroquinone (HQ), one of the phenolic metabolites of benzene, is widely recognized as an important participant in benzene-induced hematotoxicity. However, there are few relevant proteomics in HQ-induced hematotoxicity and the mechanism hasn't been fully understood yet.Methods: In this study, we treated K562 cells with 40 μmol/L HQ for 72 h, examined and validated protein expression changes by Label-free proteomic analysis and Parallel reaction monitoring (PRM), and performed bioinformatics analysis to identify interaction networks.Results: One hundred and eighty-seven upregulated differentially expressed proteins (DEPs) and 279 downregulated DEPs were identified in HQ-exposed K562 cells, which were involved in neutrophil-mediated immunity, blood microparticle, and other GO terms, as well as the lysosome, metabolic, cell cycle, and cellular senescence-related pathways. Focusing on the 23 DEGs and 5 DEPs in erythroid differentiation-related pathways, we constructed the network of protein interactions and determined 6 DEPs (STAT1, STAT3, CASP3, KIT, STAT5B, and VEGFA) as main hub proteins with the most interactions, among which STATs made a central impact and may be potential biomarkers of HQ-induced hematotoxicity.Conclusion: Our work reinforced the use of proteomics and bioinformatic approaches to advance knowledge on molecular mechanisms of HQ-induced hematotoxicity at the protein level and provide a valuable basis for further clarification.","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The First Reported Case of Co-Infection with Neisseria Meningitidisand Bordetella Pertussis in the Cerebrospinal Fluid Specimen of a Normal Four-Year-Old Child]. [脑膜炎奈瑟菌和百日咳博德特菌在四岁正常儿童脑脊液标本中合并感染的首例报告]。

Biomedical and environmental sciences : BES Pub Date : 2024-04-20 DOI: 10.3967/bes2024.048

Xian Xia, Guo Ming Li, Yi Tang, Si Quan Wang, Fei He, Yong Zhong Jiang, Hong Mei Yang, Hong Lin Jiang, Jing Lyu, Ling Feng Mao

引用次数: 0

Characterization of the CpsQ Regulon Reveals Its Role in the Transcription of Type VI Secretion System 2 Genes in Vibrio parahaemolyticus. CpsQ 调节子的特征揭示了它在副溶血性弧菌 VI 型分泌系统 2 基因转录中的作用。

Biomedical and environmental sciences : BES Pub Date : 2024-04-20 DOI: 10.3967/bes2024.049

Xi Luo, Xue Li, Miao Miao Zhang, Qing Lian Huang, Yi Quan Zhang, Shen Jie Ji, Ren Fei Lu

引用次数: 0

WNT5A Regulated by miR154-5p is Associated with Angiopoiesis and Invasion of Placental Site Trophoblastic Tumor (PSTT). 受 miR154-5p 调控的 WNT5A 与胎盘部位滋养细胞肿瘤 (PSTT) 的血管生成和侵袭有关。

Biomedical and environmental sciences : BES Pub Date : 2024-04-20 DOI: 10.3967/bes2024.046

Sai Zhang, Zhi Xian Chen, Mo Zhang, Yan Du, Jia Yi Zhou, Jing Wu, Yin Hua Yu, Qi Cao, Hong Bo Zhao

引用次数: 0