Biomedical and environmental sciences : BES最新文献

{"title":"Endometrial Cancer Research Based on Gut Microbiomics and Metabolomics: An Analysis of Correlation and Differences.","authors":"Dan Xu, Fengqin Xue, Ruifang Zhai, Sanyuan Zhang, Zhe Wang, Peiyue Yu","doi":"10.3967/bes2024.145","DOIUrl":"https://doi.org/10.3967/bes2024.145","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"37 10","pages":"1204-1207"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simulated Microgravity can Promote the Apoptosis and Change Inflammatory State of Kupffer Cells.","authors":"Jun Ge, Fei Liu, Hongyun Nie, Yuan Yue, Kaige Liu, Haiguan Lin, Hao Li, Tao Zhang, Hongfeng Yan, Bingxin Xu, Hongwei Sun, Jianwu Yang, Shaoyan Si, Jinlian Zhou, Yan Cui","doi":"10.3967/bes2024.141","DOIUrl":"https://doi.org/10.3967/bes2024.141","url":null,"abstract":"<p><strong>Objective: </strong>In this study, we analyzed the transcriptome sequences of Kupffer cells exposed to simulated microgravity for 3 d and conducted biological experiments to determine how microgravity initiates apoptosis in Kupffer cells.</p><p><strong>Methods: </strong>Rotary cell culture system was used to construct a simulated microgravity model. GO and KEGG analyses were conducted using the DAVID database. GSEA was performed using the R language. The STRING database was used to conduct PPI analysis. qPCR was used to measure the <i>IL1B, TNFA, CASP3</i>, <i>CASP9,</i> and <i>BCL2L11</i> mRNA expressions <i>.</i> Western Blotting was performed to detect the level of proteins CASP3 and CASP 9. Flow cytometry was used to detect apoptosis and mitochondrial membrane cells. Transmission electron microscopy was used to detect changes in the ultrastructure of Kupffer cells.</p><p><strong>Results: </strong>Transcriptome Sequencing indicated that simulated microgravity affected apoptosis and the inflammatory state of Kupffer cells. Simulated microgravity improved the <i>CASP3</i>, <i>CASP9,</i> and <i>BCL2L11</i> expressions in Kupffer cells. Annexin-V/ PI and JC-1 assays showed that simulated microgravity promoted apoptosis in Kupffer cells. Simulated microgravity causes M1 polarization in Kupffer cells.</p><p><strong>Conclusion: </strong>Our study found that simulated microgravity facilitated the apoptosis of Kupffer cells through the mitochondrial pathway and activated Kupffer cells into M1 polarization, which can secrete TNFA to promote apoptosis.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"37 10","pages":"1117-1127"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial Heterogeneity and Risk Factors of Dental Caries in 12-Year-Old Children in Shanxi Province, China.","authors":"Ruxia Hou, Tingting Yang, Jiajia Liu, Hao Chen, Wen Kang, Junming Li, Xiaotong Shi, Yi Liang, Junyu Liu, Bin Zhao, Xiangyu Wang","doi":"10.3967/bes2024.102","DOIUrl":"https://doi.org/10.3967/bes2024.102","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to explore the spatial heterogeneity and risk factors for dental caries in 12-year-old children in Shanxi province, China.</p><p><strong>Methods: </strong>The data encompassed 3,721 participants from the two most recent oral health surveys conducted across 16 districts in Shanxi Province in 2015 and 2018. Eighteen specific variables were analyzed to examine the interplay between socioeconomic factors, medical resources and environmental conditions. The Geo-detector model was employed to assess the impacts and interactions of these ecological factors.</p><p><strong>Results: </strong>Socioeconomic factors ( <i>Q</i> = 0.30, <i>P</i> < 0.05) exhibited a more substantial impact compared to environmental ( <i>Q</i> = 0.19, <i>P</i> < 0.05) and medical resource factors ( <i>Q</i> = 0.25, <i>P</i> < 0.05). Notably, the urban population percentage (UPP) demonstrated the most significant explanatory power for the spatial heterogeneity in caries prevalence, as denoted by its highest <i>q</i>-value ( <i>q</i> = 0.51, <i>P</i> < 0.05). Additionally, the spatial distribution's heterogeneity of caries was significantly affected by SO ₂ concentration ( <i>q</i> = 0.39, <i>P</i> < 0.05) and water fluoride levels ( <i>q</i> = 0.27, <i>P</i> < 0.05) among environmental factors.</p><p><strong>Conclusion: </strong>The prevalence of caries exhibited spatial heterogeneity, escalating from North to South in Shanxi Province, China, influenced by socioeconomic factors, medical resources, and environmental conditions to varying extents.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"37 10","pages":"1173-1183"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Blood Lipid Profiles on Chronic Kidney Disease in a Prospective Cohort: Based on a Regression Discontinuity Design.","authors":"Kang Lyu, Shaodong Liu, Yanli Liu, Jinlong You, Xue Wang, Min Jiang, Chun Yin, Desheng Zhang, Yana Bai, Minzhen Wang, Shan Zheng","doi":"10.3967/bes2024.113","DOIUrl":"https://doi.org/10.3967/bes2024.113","url":null,"abstract":"<p><strong>Objective: </strong>Previous studies on the association between lipid profiles and chronic kidney disease (CKD) have yielded inconsistent results and no defined thresholds for blood lipids.</p><p><strong>Methods: </strong>A prospective cohort study including 32,351 subjects who completed baseline and follow-up surveys over 5 years was conducted. Restricted cubic splines and Cox models were used to examine the association between the lipid profiles and CKD. A regression discontinuity design was used to determine the cutoff value of lipid profiles that was significantly associated with increased the risk of CKD.</p><p><strong>Results: </strong>Over a median follow-up time of 2.2 (0.5, 4.2) years, 648 (2.00%) subjects developed CKD. The lipid profiles that were significantly and linearly related to CKD included total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), TC/HDL-C, and TG/HDL-C, whereas low-density lipoprotein cholesterol (LDL-C) and LDL-C/HDL-C were nonlinearly correlated with CKD. TC, TG, TC/HDL-C, and TG/HDL-C showed an upward jump at the cutoff value, increasing the risk of CKD by 0.90%, 1.50%, 2.30%, and 1.60%, respectively, whereas HDL-C showed a downward jump at the cutoff value, reducing this risk by 1.0%. Female and participants with dyslipidemia had a higher risk of CKD, while the cutoff values for the different characteristics of the population were different.</p><p><strong>Conclusion: </strong>There was a significant association between lipid profiles and CKD in a prospective cohort from Northwest China, while TG, TC/HDL-C, and TG/HDL-C showed a stronger risk association. The specific cutoff values of lipid profiles may provide a clinical reference for screening or diagnosing CKD risk.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"37 10","pages":"1158-1172"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilm Elimination Effect of Baicalein-Loaded Hyaluronic acid/β-Cyclodextrin grafted Chitosan Nanoparticles.","authors":"Liting Lai, Wenyou Ding, Guoying Huang, Mengke Wang, Jinqing Chen, Liuzhen Lai, Xiuzhen Deng, Ling Tang, Xinyi Yu, Ya Huang, Thi Minh Hien Truong, Zhongbin Zhang, Wenya Ding","doi":"10.3967/bes2024.160","DOIUrl":"https://doi.org/10.3967/bes2024.160","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"37 10","pages":"1227-1231"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Seasonal and Temperature Variations on the Association between Nitrogen Dioxide Exposure and First-Aid Incidence for Neurological Diseases in Shenzhen, China.","authors":"Tongyu Yang, Qingqing Wang, Yuchen Tian, Siyi Chen, Yilei Ma, Peng Wang, Suli Huang, Ji Peng, Jinquan Cheng, Jing Hu, Ping Yin","doi":"10.3967/bes2024.147","DOIUrl":"https://doi.org/10.3967/bes2024.147","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"37 10","pages":"1213-1220"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Safety of Combined Bedaquiline and Delamanid Use among Patients with Multidrug-Resistant Tuberculosis in Beijing, China.","authors":"Can Guo, Lihui Nie, Yanhua Song, Rongmei Liu, Xiaoguang Wu, Yuanyuan Shang, Xuxia Zhang, Yu Pang, Mengqiu Gao","doi":"10.3967/bes2024.088","DOIUrl":"https://doi.org/10.3967/bes2024.088","url":null,"abstract":"<p><strong>Objectives: </strong>The combined use of bedaquiline and delamanid (BDQ-DLM) is limited by an increased risk of prolonging the QTc interval. We retrospectively evaluated patients who received DLM/BDQ-containing regimens at a TB-specialized hospital. We aimed to present clinical efficacy and safety data for Chinese patients.</p><p><strong>Methods: </strong>This case-control study included patients with multidrug-resistant tuberculosis (MDR-TB) treated with BDQ alone or BDQ plus DLM.</p><p><strong>Results: </strong>A total of 96 patients were included in this analysis: 64 in the BDQ group and 32 in the BDQ + DLM group. Among the 96 patients with positive sputum culture at the initiation of BDQ alone or BDQ combined with DLM, 46 patients (71.9%) in the BDQ group and 29 (90.6%) in the BDQ-DLM group achieved sputum culture conversion during treatment. The rate of sputum culture conversion did not differ between the two groups. The time to sputum culture conversion was significantly shorter in the BDQ-DLM group than in the BDQ group. The most frequent adverse event was QTc interval prolongation; however, the frequency of adverse events did not differ between the groups.</p><p><strong>Conclusion: </strong>In conclusion, our results demonstrate that the combined use of BDQ and DLM is efficacious and tolerable in Chinese patients infected with MDR-TB. Patients in the BDQ-DLM group achieved sputum culture conversion sooner than those in the BDQ group.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"37 10","pages":"1195-1203"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Metal(loid) Exposure and Risk of Polycystic Ovary Syndrome Mediated by Anti-Müllerian Hormone among Women Undergoing <i>In Vitro</i> Fertilization and Embryo Transfer.","authors":"Shu Su, Mengyuan Ren, Yanqiu Feng, Changxin Lan, Lailai Yan, Qun Lu, Jia Xu, Bin Han, Lili Zhuang, Mingliang Fang, Bin Wang, Hongchu Bao, Bo Pan","doi":"10.3967/bes2024.154","DOIUrl":"https://doi.org/10.3967/bes2024.154","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the relationship and potential pathways between metal(loid) exposure and the risk of polycystic ovary syndrome (PCOS) in women of childbearing age.</p><p><strong>Methods: </strong>This case-control study included 200 patients with PCOS (cases) and 896 non-PCOS controls with the age of 25-37 years. The concentrations of 29 metal(loid)s in the follicular fluid (FF) and clinical indicators in the serum were measured in all participants. Logistic regression analysis and mediation analysis were conducted to evaluate the associations between metal(loid) exposure and PCOS risk and investigate the possible roles of clinical indicators, respectively.</p><p><strong>Results: </strong>Logistic regression analysis revealed an association between high copper levels in FF and increased PCOS risk (highest <i>vs.</i> lowest quartile: adjusted odds ratio = 2.94, 95% confidence interval: 1.83-4.72). A high luteinizing hormone/follicle-stimulating hormone ratio and elevated levels of testosterone and anti-Müllerian hormone (AMH) were strongly associated with increased PCOS risk induced by high copper exposure. The mediation analysis indicated a mediating effect of AMH in the association between copper exposure and PCOS risk.</p><p><strong>Conclusion: </strong>Copper may affect PCOS risk through the hypothalamic-pituitary-ovarian axis, mediated by AMH. Copper exposure and internal AMH levels are important indicators for early warning of PCOS development.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"37 10","pages":"1107-1116"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mito-TEMPO Ameliorates Sodium Palmitate Induced Ferroptosis in MIN6 Cells through PINK1/Parkin-Mediated Mitophagy.","authors":"Baolei Chang, Yanyu Su, Tingting Li, Yanxia Zheng, Ruirui Yang, Heng Lu, Hao Wang, Yusong Ding","doi":"10.3967/bes2024.111","DOIUrl":"https://doi.org/10.3967/bes2024.111","url":null,"abstract":"<p><strong>Objective: </strong>Mitochondrial reactive oxygen species (mtROS) could cause damage to pancreatic β-cells, rendering them susceptible to oxidative damage. Hence, investigating the potential of the mitochondria-targeted antioxidant (Mito-TEMPO) to protect pancreatic β-cells from ferroptosis by mitigating lipid peroxidation becomes crucial.</p><p><strong>Methods: </strong>MIN6 cells were cultured <i>in</i> <i>vitro</i> with 100 μmol/L sodium palmitate (SP) to simulate diabetes. FerroOrange was utilized for the detection of Fe ²⁺ fluorescence staining, BODIPY581/591C11 for lipid reactive oxygen species, and MitoSox-Red for mtROS. Alterations in mitophagy levels were assessed through the co-localization of lysosomal and mitochondrial fluorescence. Western blotting was employed to quantify protein levels of Acsl4, GPX4, FSP1, FE, PINK1, Parkin, TOMM20, P62, and LC3. Subsequently, interventions were implemented using Mito-TEMPO and Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) to observe changes in ferroptosis and mitophagy within MIN6 cells.</p><p><strong>Results: </strong>We found that SP induced a dose-dependent increase in Fe ²⁺ and lipid ROS in MIN6 cells while decreasing the expression levels of GPX4 and FSP1 proteins. Through bioinformatics analysis, it has been uncovered that mitophagy assumes a crucial role within the ferroptosis pathway associated with diabetes. Additionally, SP decreased the expression of mitophagy-related proteins PINK1 and Parkin, leading to mtROS overproduction. Conversely, Mito-TEMPO effectively eliminated mtROS while activating the mitophagy pathways involving PINK1 and Parkin, thereby reducing the occurrence of ferroptosis in MIN6 cells. CCCP also demonstrated efficacy in reducing ferroptosis in MIN6 cells.</p><p><strong>Conclusion: </strong>In summary, Mito-TEMPO proved effective in attenuating mtROS production and initiating mitophagy pathways mediated by PINK1 and Parkin in MIN6 cells. Consequently, this decreased iron overload and lipid peroxidation, ultimately safeguarding the cells from ferroptosis.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"37 10","pages":"1128-1141"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thyroid Nodule Prevalence and Iodine Nutrition: Influencing Factors in Coastal Areas.","authors":"Lijin Wang, Jiani Wu, Xiaoyan Wu, Ying Lan, Meng He, Diqun Chen, Zhihui Chen, Muhua Wang","doi":"10.3967/bes2024.164","DOIUrl":"https://doi.org/10.3967/bes2024.164","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"37 10","pages":"1221-1226"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}