Jia Yan Zhang, Meng Ting Liu, Yu Hao Liu, Huan Deng, Juan Bai, Jian Hua Xie, Xiang Xiao
{"title":"Barley Protein LFBEP-C1 from <i>Lactiplantibacillus plantarum dy-1</i> Fermented Barley Extracts by Inhibiting Lipid Accumulation in a <i>Caenorhabditis elegans</i> Model.","authors":"Jia Yan Zhang, Meng Ting Liu, Yu Hao Liu, Huan Deng, Juan Bai, Jian Hua Xie, Xiang Xiao","doi":"10.3967/bes2024.042","DOIUrl":"10.3967/bes2024.042","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the lipid-lowering activity of LFBEP-C1 in high glucose-fed <i>Caenorhabditis elegans (C. elegans)</i>.</p><p><strong>Methods: </strong>In this study, the fermented barley protein LFBEP-C1 was prepared and tested for its potential anti-obesity effects on <i>C. elegans</i>. The worms were fed <i>Escherichia coli</i> OP50 ( <i>E. coli</i> OP50), glucose, and different concentrations of LFBEP-C1. Body size, lifespan, movement, triglyceride content, and gene expression were analyzed. The results were analyzed using ANOVA and Tukey's multiple comparison test.</p><p><strong>Results: </strong>Compared with the model group, the head-swing frequency of <i>C. elegans</i> in the group of LFBEP-C1 at 20 μg/mL increased by 33.88%, and the body-bending frequency increased by 27.09%. This indicated that LFBEP-C1 improved the locomotive ability of <i>C. elegans.</i> The average lifespan of <i>C. elegans</i> reached 13.55 days, and the body length and width of the <i>C. elegans</i> decreased after LFBEP-C1 intake. Additionally, LFBEP-C1 reduced the content of lipid accumulation and triglyceride levels. The expression levels of <i>sbp-1</i>, <i>daf-2</i>, and <i>mdt-15</i> significantly decreased, while those of <i>daf-16</i>, <i>tph-1</i>, <i>mod-1</i>, and <i>ser-4</i> significantly increased after LFBEP-C1 intake. Changes in these genes explain the signaling pathways that regulate lipid metabolism.</p><p><strong>Conclusion: </strong>LFBEP-C1 significantly reduced lipid deposition in <i>C. elegans</i> fed a high-glucose diet and alleviated the adverse effects of a high-glucose diet on the development, lifespan, and exercise behavior of <i>C. elegans</i>. In addition, LFBEP-C1 regulated lipid metabolism mainly by mediating the expression of genes in the sterol regulatory element-binding protein, insulin, and 5-hydroxytryptamine signaling pathways.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140899977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hong Yang, Yi Sun, Shu Tong Chai, Yan Hui Liang, Ya Ni Liu, Guan E Yang
{"title":"Therapeutic Effect of <i>Rhodopseudomonas palustris</i>-Bioconverted Extract of <i>Lycium barbarum</i> on Rats with Lead Exposure.","authors":"Hong Yang, Yi Sun, Shu Tong Chai, Yan Hui Liang, Ya Ni Liu, Guan E Yang","doi":"10.3967/bes2024.050","DOIUrl":"https://doi.org/10.3967/bes2024.050","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jun Zhou, Yan Yan, Ya Wei Dong, Xiao Yu Liu, Ping Wang, Jia Ming Lin, Peng Xuan Dong, Rui Quan, Rong Tian Wang, Bao Hong Mi, Wei Heng Chen
{"title":"Prevalence, Characteristics and Treatment of Knee Osteoarthritis in Urban Residents of Beijing, China: A Community-Based Cross-Sectional Study.","authors":"Jun Zhou, Yan Yan, Ya Wei Dong, Xiao Yu Liu, Ping Wang, Jia Ming Lin, Peng Xuan Dong, Rui Quan, Rong Tian Wang, Bao Hong Mi, Wei Heng Chen","doi":"10.3967/bes2024.045","DOIUrl":"https://doi.org/10.3967/bes2024.045","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zi Jin Zhao, Xiao Ping Chen, Shao Wei Hua, Feng Yu Li, Meng Zhao, Chen Hao Xing, Jie Wang, Feng Yu Tian, Rui Qing Zhang, Xiao Na Lyu, Zhi Qiang Han, Yu Xin Wang, Hong Yi Li, Xin Xin Shen, Xue Jun Ma, Yan Qing Tie
{"title":"Establishment of a Multiplex Detection Method for Common Bacteria in Blood Based on Human Mannan-Binding Lectin Protein-Conjugated Magnetic Bead Enrichment Combined with Recombinase-Aided PCR Technology.","authors":"Zi Jin Zhao, Xiao Ping Chen, Shao Wei Hua, Feng Yu Li, Meng Zhao, Chen Hao Xing, Jie Wang, Feng Yu Tian, Rui Qing Zhang, Xiao Na Lyu, Zhi Qiang Han, Yu Xin Wang, Hong Yi Li, Xin Xin Shen, Xue Jun Ma, Yan Qing Tie","doi":"10.3967/bes2024.043","DOIUrl":"10.3967/bes2024.043","url":null,"abstract":"<p><strong>Objective: </strong>Recombinase-aided polymerase chain reaction (RAP) is a sensitive, single-tube, two-stage nucleic acid amplification method. This study aimed to develop an assay that can be used for the early diagnosis of three types of bacteremia caused by <i>Staphylococcus aureus</i> (SA), <i>Pseudomonas aeruginosa</i> (PA), and <i>Acinetobacter baumannii</i> (AB) in the bloodstream based on recombinant human mannan-binding lectin protein (M1 protein)-conjugated magnetic bead (M1 bead) enrichment of pathogens combined with RAP.</p><p><strong>Methods: </strong>Recombinant plasmids were used to evaluate the assay sensitivity. Common blood influenza bacteria were used for the specific detection. Simulated and clinical plasma samples were enriched with M1 beads and then subjected to multiple recombinase-aided PCR (M-RAP) and quantitative PCR (qPCR) assays. Kappa analysis was used to evaluate the consistency between the two assays.</p><p><strong>Results: </strong>The M-RAP method had sensitivity rates of 1, 10, and 1 copies/μL for the detection of SA, PA, and AB plasmids, respectively, without cross-reaction to other bacterial species. The M-RAP assay obtained results for < 10 CFU/mL pathogens in the blood within 4 h, with higher sensitivity than qPCR. M-RAP and qPCR for SA, PA, and AB yielded Kappa values of 0.839, 0.815, and 0.856, respectively ( <i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>An M-RAP assay for SA, PA, and AB in blood samples utilizing M1 bead enrichment has been developed and can be potentially used for the early detection of bacteremia.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yu Shan Hou, Yi Chen Jin, Chang Cai, Hou Lin Tang, Qian Qian Qin, Fan Lyu
{"title":"Characteristics of the HIV/AIDS Epidemic among People Aged ≥ 50 Years in China during 2018-2021.","authors":"Yu Shan Hou, Yi Chen Jin, Chang Cai, Hou Lin Tang, Qian Qian Qin, Fan Lyu","doi":"10.3967/bes2024.044","DOIUrl":"https://doi.org/10.3967/bes2024.044","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to determine the current epidemiological status of PLWHA aged ≥ 50 years in China from 2018 to 2021. It also aimed to recommend targeted interventions for the prevention and treatment of HIV/AIDS in elderly patients.</p><p><strong>Methods: </strong>Data on newly reported cases of PLWHA, aged ≥ 50 years in China from 2018 to 2021, were collected using the CRIMS. Trend tests and spatial analyses were also conducted.</p><p><strong>Results: </strong>Between 2018 and 2021, 237,724 HIV/AIDS cases were reported among patients aged ≥ 50 years in China. The main transmission route was heterosexual transmission (91.24%). Commercial heterosexual transmission (CHC) was the primary mode of transmission among males, while non-marital non-CHC ([NMNCHC]; 60.59%) was the prevalent route in women. The proportion of patients with CHC decreased over time ( <i>Z</i> = 67.716, <i>P</i> < 0.01), while that of patients with NMNCHC increased ( <i>Z</i> = 153.05, <i>P</i> < 0.01). The sex ratio varied among the different modes of infection, and it peaked at 17.65 for CHC. The spatial analysis indicated spatial clustering, and the high-high clustering areas were mainly distributed in the southwestern and central-southern provinces.</p><p><strong>Conclusion: </strong>In China, PLWHA, aged ≥ 50 years, were predominantly infected through heterosexual transmission. The primary modes of infection were CHC and NMNCHC. There were variations in the sex ratio among different age groups, infected through various sexual behaviors. HIV/AIDS cases exhibited spatial clustering. Based on these results, the expansion of HIV testing, treatment, and integrated behavioral interventions in high-risk populations is recommended to enhance disease detection in key regions.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yi Shan Jin, Zong Chun Yi, Yu Jing Zhang, Long Rong, Chun Hong Yu
{"title":"Proteomics Study of Benzene Metabolite Hydroquinone Induced Hematotoxicity in K562 Cells.","authors":"Yi Shan Jin, Zong Chun Yi, Yu Jing Zhang, Long Rong, Chun Hong Yu","doi":"10.3967/bes2024.039","DOIUrl":"10.3967/bes2024.039","url":null,"abstract":"<p><strong>Objective: </strong>Hydroquinone (HQ), one of the phenolic metabolites of benzene, is widely recognized as an important participant in benzene-induced hematotoxicity. However, there are few relevant proteomics in HQ-induced hematotoxicity and the mechanism hasn't been fully understood yet.</p><p><strong>Methods: </strong>In this study, we treated K562 cells with 40 μmol/L HQ for 72 h, examined and validated protein expression changes by Label-free proteomic analysis and Parallel reaction monitoring (PRM), and performed bioinformatics analysis to identify interaction networks.</p><p><strong>Results: </strong>One hundred and eighty-seven upregulated differentially expressed proteins (DEPs) and 279 downregulated DEPs were identified in HQ-exposed K562 cells, which were involved in neutrophil-mediated immunity, blood microparticle, and other GO terms, as well as the lysosome, metabolic, cell cycle, and cellular senescence-related pathways. Focusing on the 23 DEGs and 5 DEPs in erythroid differentiation-related pathways, we constructed the network of protein interactions and determined 6 DEPs (STAT1, STAT3, CASP3, KIT, STAT5B, and VEGFA) as main hub proteins with the most interactions, among which STATs made a central impact and may be potential biomarkers of HQ-induced hematotoxicity.</p><p><strong>Conclusion: </strong>Our work reinforced the use of proteomics and bioinformatic approaches to advance knowledge on molecular mechanisms of HQ-induced hematotoxicity at the protein level and provide a valuable basis for further clarification.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xian Xia, Guo Ming Li, Yi Tang, Si Quan Wang, Fei He, Yong Zhong Jiang, Hong Mei Yang, Hong Lin Jiang, Jing Lyu, Ling Feng Mao
{"title":"[The First Reported Case of Co-Infection with <i>Neisseria</i> <i>Meningitidis</i>and <i>Bordetella Pertussis</i> in the Cerebrospinal Fluid Specimen of a Normal Four-Year-Old Child].","authors":"Xian Xia, Guo Ming Li, Yi Tang, Si Quan Wang, Fei He, Yong Zhong Jiang, Hong Mei Yang, Hong Lin Jiang, Jing Lyu, Ling Feng Mao","doi":"10.3967/bes2024.048","DOIUrl":"10.3967/bes2024.048","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140899761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xi Luo, Xue Li, Miao Miao Zhang, Qing Lian Huang, Yi Quan Zhang, Shen Jie Ji, Ren Fei Lu
{"title":"Characterization of the CpsQ Regulon Reveals Its Role in the Transcription of Type VI Secretion System 2 Genes in <i>Vibrio parahaemolyticus</i>.","authors":"Xi Luo, Xue Li, Miao Miao Zhang, Qing Lian Huang, Yi Quan Zhang, Shen Jie Ji, Ren Fei Lu","doi":"10.3967/bes2024.049","DOIUrl":"10.3967/bes2024.049","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sai Zhang, Zhi Xian Chen, Mo Zhang, Yan Du, Jia Yi Zhou, Jing Wu, Yin Hua Yu, Qi Cao, Hong Bo Zhao
{"title":"WNT5A Regulated by miR154-5p is Associated with Angiopoiesis and Invasion of Placental Site Trophoblastic Tumor (PSTT).","authors":"Sai Zhang, Zhi Xian Chen, Mo Zhang, Yan Du, Jia Yi Zhou, Jing Wu, Yin Hua Yu, Qi Cao, Hong Bo Zhao","doi":"10.3967/bes2024.046","DOIUrl":"10.3967/bes2024.046","url":null,"abstract":"","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}