Biomaterials research最新文献

{"title":"Evolution of Biological Hydroxyapatite Modification Strategy: Anti-Inflammation Approach Rescues the Calcium-NOD-Like Receptor-Inflammation Axis-Mediated Periodontal Redevelopment Failure.","authors":"Xuan Zhou, Junlong Xue, Yanshu Zhang, Ruidi Xia, Zhengjie Shan, Linjun Zhang, Mixiao Gui, Guanqi Liu, Zetao Chen","doi":"10.34133/bmr.0131","DOIUrl":"10.34133/bmr.0131","url":null,"abstract":"<p><p>Periodontal regenerative medicine is currently undergoing a paradigm shift from dissecting the healing process toward utilization of the developmental program. Biological hydroxyapatite (BHA), a major component of guided tissue regeneration, has long been optimized for inducing multidirectional differentiation of periodontal ligament cells (PDLCs). However, this approach runs counter to the redevelopment strategy. Thus, the conventional BHA should evolve to induce the redevelopment process of periodontal tissue. In this study, histopathological changes and immune microenvironment characteristics of the periodontal developmental process, natural healing process (Blank group), and BHA-mediated healing process (BHA group) were compared to evaluate the main manifestations of BHA-mediated periodontal \"developmental engineering\" outcome. Our results suggested that neither the Blank nor BHA group could recur key events in periodontal development. The implantation of BHA led to pro-inflammatory immune microenvironment and an unstable blood fibrin clot structure, which facilitated the invasion of outer gingival fibroblasts, consequently disrupting redevelopmental events. High-throughput chip technology was further used to explore the underlying mechanism of immune activation, revealing that the calcium-NOD-like receptor-inflammation axis signaling axis promoted the activation of pro-inflammatory immune response that contributed to redevelopment failure. An immunomodulatory cytokine interleukin 4 (IL4)-modified BHA was used to further validate the efficacy of developmental engineering strategy. IL4 could partially rescued the redevelopment failure through immunosuppression. This study presented an innovative strategy for the development of advanced periodontal regenerative materials and offered a potential approach for the application of development-inspired periodontal tissue engineering strategies. It represented a marked advancement in the development of regenerative medicine and propelled the clinical organ restoration forward.</p>","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0131"},"PeriodicalIF":8.1,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11862812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143517612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boron Nanocomposites for Boron Neutron Capture Therapy and in Biomedicine: Evolvement and Challenges.","authors":"Farooq Ahmad","doi":"10.34133/bmr.0145","DOIUrl":"10.34133/bmr.0145","url":null,"abstract":"<p><p>Cancer remains a major concern for human health worldwide. To fight the curse of cancer, boron neutron capture therapy is an incredibly advantageous modality in the treatment of cancer as compared to other radiotherapies. Due to tortuous vasculature in and around tumor regions, boron (¹⁰B) compounds preferentially house into tumor cells, creating a large dose gradient between the highly mingled cancer cells and normal cells. Epithermal or thermal neutron bombardment leads to tumor-cell-selective killing due to the generation of heavy particles yielded from in situ fission reaction. However, the major challenges for boron nanocomposites' development have been from the synthesis part as well as the requirement for selective cancer targeting and the delivery of therapeutic concentrations of boron (¹⁰B) with nominal healthy tissue accumulation and retention. To circumvent the above challenges, this review discusses boride nanocomposite design, safety, and biocompatibility for biomedical applications for general public use. This review sparks interest in using boron nanocomposites as boron neutron capture therapy agents and repurposing them in comorbidity treatments, with future scientific challenges and opportunities, with a hope to accelerate the stimulus of developing possible boron composite nanomedicine research and applications worldwide.</p>","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0145"},"PeriodicalIF":8.1,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin-Like Growth Factor 2 Secreted from Mesenchymal Stem Cells with High Glutathione Levels Alleviates Osteoarthritis via Paracrine Rejuvenation of Senescent Chondrocytes.","authors":"Gun Hee Cho, Hyun Cheol Bae, Yu Jeong Lee, Ha Ru Yang, Hyewon Kang, Hee Jung Park, Sun Young Wang, You Jung Kim, Heun-Soo Kang, In Gyu Kim, Byung Sun Choi, Hyuk-Soo Han","doi":"10.34133/bmr.0152","DOIUrl":"10.34133/bmr.0152","url":null,"abstract":"<p><p>Senescent chondrocytes, which are increased in osteoarthritic (OA) cartilage, promote cartilage defects and the senescent knee microenvironment by inducing senescence to surrounding normal chondrocytes by secreting senescence-associated secretory proteins. Many studies have used mesenchymal stem cells (MSCs) to treat OA, but MSC treatment remains challenging for clinical application owing to MSC quality control, engraftment, and fibrocartilage regeneration. Here, rather than relying on the direct regeneration of MSCs, we present a novel strategy to suppress OA by MSC-mediated senescent chondrocyte targeting via the paracrine activity of MSCs, thereby improving the knee microenvironment. First, to enable quality control of umbilical cord MSCs, priming MSCs by supplementing human platelet lysate (hPL) greatly enhanced MSC functions by increasing cellular glutathione levels throughout serial passaging. Intra-articular injection of primed MSCs successfully suppressed OA progression and senescent chondrocyte accumulation without direct regeneration. Indirect coculture with primed MSCs using transwell ameliorated the senescence phenotypes in OA chondrocytes, suggesting paracrine rejuvenation. Based on secretome analysis, we identified insulin-like growth factor 2 (IGF2) as a key component that induces paracrine rejuvenation by primed MSCs. The rejuvenation effects of IGF2 act through autophagy activation through the up-regulation of autophagy-related gene expression and autophagic flux. To cross-validate the effects of secreted IGF2 in vivo, knockdown of IGF2 in primed MSCs substantially abolished its therapeutic efficacy in a rabbit OA model. Collectively, these findings demonstrate that hPL supplementation enables MSC quality control by increasing MSC glutathione levels. The therapeutic mechanism of primed MSCs was secreted IGF2, which induces paracrine rejuvenation of senescent OA chondrocytes by activating autophagy.</p>","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0152"},"PeriodicalIF":8.1,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Therapeutic Potential of Extracellular Vesicles Anchored to the Sea Cucumber Extracellular Matrix for Treating Atopic Dermatitis.","authors":"Sung-Han Jo, Seon-Hwa Kim, Su Chin Heo, Hongsik Cho, Iman Janghorban Esfahani, Sang-Hyug Park","doi":"10.34133/bmr.0154","DOIUrl":"10.34133/bmr.0154","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) are crucial for intercellular communication and affect various physiological and pathological processes. Although terrestrial EVs have been extensively studied, marine-derived EVs have yet to be explored. This study investigated the therapeutic potential of sea cucumbers, known for their regenerative and immune abilities. Sea cucumber extracellular matrix (ECM)-anchored EVs (SEVs) were isolated and characterized using physical and electrophoretic analyses. Morphological assessments have shown that SEVs have shape and size distributions similar to mammalian EVs. Internal cargo analysis revealed the encapsulation of diverse proteins and genetic molecules. In anti-inflammatory tests with a lipopolysaccharide (LPS)-induced macrophage model, the results have shown that SEVs can alleviate inflammation factors regarding inducible nitric oxide synthase (iNOS) protein and immune-related mRNA expression. Microarray analysis was conducted to elucidate SEV's pharmacological efficacy and anti-inflammatory mechanisms, showing that SEVs inhibit the nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) signaling pathway. An in vivo study using a mouse model of atopic dermatitis (AD) induced by 2,4-dinitrochlorobenzene (DNCB) involved subcutaneous SEV administration, followed by severity scoring and histological analyses. Therapeutic efficacy analysis indicated improvements in the AD mouse models, including reduced skin thickness and mast cell numbers. These findings indicate their potential for treating AD. This study highlights the potential clinical applications of marine-derived EVs and offers important implications for future research and therapeutic developments.</p>","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0154"},"PeriodicalIF":8.1,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Dual Ag/Cu Ion Implantation to Enhance Antimicrobial Defense on Boston Keratoprosthesis.","authors":"Silvia G Gómez, Gerard Boix-Lemonche, Jordi Orrit-Prat, Raül Bonet, Jaume Caro, Joan Muñoz, Maria-Pau Ginebra, Barbara Skerlavaj, Rafael I Barraquer, José M Manero","doi":"10.34133/bmr.0147","DOIUrl":"10.34133/bmr.0147","url":null,"abstract":"<p><p>The Boston keratoprosthesis (BKPro) is a critical device for vision restoration in complex cases of corneal blindness, although its long-term retention is challenged by infection risks. This study aims to enhance the antimicrobial properties of the titanium (Ti) backplate of the BKPro by ion implanting silver and copper ions to achieve effective infection control while maintaining cytocompatibility. Research on antimicrobial modifications for BKPro is limited, and while metallic ions like Ag and Cu show promise for biomaterial improvement, their effects on human corneal keratocytes (HCKs) require further study. Ag and Cu were implanted onto rough Ti surfaces, as mono- and coimplantations. Cytotoxicity was assessed in HCKs, and antimicrobial efficacy was tested against <i>Pseudomonas aeruginosa</i> and <i>Candida albicans</i>. After 21 d, monoimplanted Ag samples released 300.4 ppb of Ag⁺, coimplanted samples released 427.5 ppb of Ag⁺ and 272.3 ppb of Cu ions, and monoimplanted Cu samples released 567.0 ppb of Cu ions. All ion-implanted surfaces supported HCK proliferation, exhibited no cytotoxicity, and showed strong antimicrobial activity. Ag-implanted surfaces provided antibacterial effects through membrane disruption and reactive oxygen species generation, while Cu-implanted surfaces exhibited antifungal effects via impaired enzymatic functions and reactive oxygen species. Coimplanted AgCu surfaces demonstrated synergistic antimicrobial effects, resulting from the synergy between the bactericidal actions of Ag and the oxidative stress contributions of Cu. Additionally, ion-implanted surfaces enhanced HCK adhesion under co-culture conditions. In conclusion, ion implantation effectively imparts antimicrobial properties to the Ti backplate of BKPro, reducing infection risks while preserving compatibility with corneal cells.</p>","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0147"},"PeriodicalIF":8.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11836197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143461074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Dimensional Printed Cell-Adaptable Nanocolloidal Hydrogel Induces Endogenous Osteogenesis for Bone Repair.","authors":"Wenxin Lu, Li Li, Ruyi Wang, Yanting Wu, Yao Chen, Bowen Tan, Zhihe Zhao, Maling Gou, Yu Li","doi":"10.34133/bmr.0146","DOIUrl":"10.34133/bmr.0146","url":null,"abstract":"<p><p>Repairing critical bone defects remains a formidable challenge in regenerative medicine. Scaffolds that can fill defects and facilitate bone regeneration have garnered considerable attention. However, scaffolds struggle to provide an ideal microenvironment for cell growth and differentiation at the interior of the bone defect sites. The scaffold's structure must meet specific requirements to support endogenous bone regeneration. Here, we introduce a novel 3D-printed nanocolloidal gelatin methacryloyl (GelMA) hydrogel, namely, the nG hydrogel, that was derived from the self-assembly of GelMA in the presence of Pluronics F68, emphasizing its osteoinductive capability conferred solely by the specific nanocolloidal structure. The nG hydrogel, exhibiting remarkable pore connectivity and cell-adaptable microscopic structure, induced the infiltration and migration of rat bone mesenchymal stem cells (rBMSCs) into the hydrogel with a large spreading area in vitro. Moreover, the nG hydrogel with interconnected nanospheres promoted the osteogenic differentiation of rBMSCs, leading to up-regulated expression of ALP, RUNX2, COL-1, and OCN, as well as augmented formation of calcium nodules. In the critical-sized rat calvarial defect model, the nG hydrogel demonstrated improved repair of bone defects, with enhanced recruitment of endogenous CD29⁺ and CD90⁺ stem cells and increased bone regeneration, as indicated by significantly higher bone mineral density (BMD) in vivo. Mechanistically, the integrin β1/focal adhesion kinase (FAK) mechanotransduction signaling pathway was up-regulated in the nG hydrogel group both in vitro and in vivo, which may partially account for its pronounced osteoinductive capability. In conclusion, the cell-adaptable nG hydrogel shows great potential as a near-future clinical translational strategy for the customized repair of critical-sized bone defects.</p>","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0146"},"PeriodicalIF":8.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11825971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid Nanoparticle Delivery System for Normalization of Tumor Microenvironment and Tumor Vascular Structure.","authors":"Heejin Ha, Yonghyun Choi, Na-Hyeon Kim, Jiwon Kim, Jaehee Jang, Tagbo H R Niepa, Masayoshi Tanaka, Hee-Young Lee, Jonghoon Choi","doi":"10.34133/bmr.0144","DOIUrl":"10.34133/bmr.0144","url":null,"abstract":"<p><p>Tumors grow by receiving oxygen and nutrients from the surrounding blood vessels, leading to rapid angiogenesis. This results in functionally and structurally abnormal vasculature characterized by high permeability and irregular blood flow, causing hypoxia within the tumor microenvironment (TME). Hypoxia exacerbates the secretion of pro-angiogenic factors such as vascular endothelial growth factor (VEGF), further perpetuating abnormal vessel formation. This environment compromises the efficacy of radiotherapy, immunotherapy, and chemotherapy. In this study, we developed a pH-sensitive liposome (PSL) system, termed OD_PSL@AKB, to co-deliver oxygen (OD) and razuprotafib (AKB-9778) to tumors. This system rapidly responds to the acidic TME to alleviate hypoxia and inhibit VEGF secretion, restoring VE-cadherin expression in hypoxic endothelial cell/cancer cell cocultures. Our findings highlight the potential of OD_PSL@AKB in normalizing tumor vasculature and improving therapeutic efficacy.</p>","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0144"},"PeriodicalIF":8.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MXene-Derived Multifunctional Biomaterials: New Opportunities for Wound Healing.","authors":"Dong Luo, Hui-Qi Zhang, Xin-Yang Xuanyuan, Dan Deng, Zheng-Mao Lu, Wen-Shang Liu, Meng Li","doi":"10.34133/bmr.0143","DOIUrl":"10.34133/bmr.0143","url":null,"abstract":"<p><p>The process of wound healing is frequently impeded by metabolic imbalances within the wound microenvironment. MXenes exhibit exceptional biocompatibility, biodegradability, photothermal conversion efficiency, conductivity, and adaptable surface functionalization, demonstrating marked potential in the development of multifunctional platforms for wound healing. Moreover, the integration of MXenes with other bioactive nanomaterials has been shown to enhance their therapeutic efficacy, paving the way for innovative approaches to wound healing. In this review, we provide a systematic exposition of the mechanisms through which MXenes facilitate wound healing and offer a comprehensive analysis of the current research landscape on MXene-based multifunctional bioactive composites in this field. By delving into the latest scientific discoveries, we identify the existing challenges and potential future trajectories for the advancement of MXenes. Our comprehensive evaluation aims to provide insightful guidance for the formulation of more effective wound healing strategies.</p>","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0143"},"PeriodicalIF":8.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Bioactive Glass into Mineral Trioxide Aggregate on the Biocompatibility and Mineralization Potential of Dental Pulp Stem Cells.","authors":"Hee-Gyun Kim, Bin-Na Lee, Hyun-Jeong Jeong, Hyun-Jung Kim, Jiyoung Kwon, Soram Oh, Duck-Su Kim, Kyoung-Kyu Choi, Reuben H Kim, Ji-Hyun Jang","doi":"10.34133/bmr.0142","DOIUrl":"10.34133/bmr.0142","url":null,"abstract":"<p><p><b>Introduction:</b> Previous studies have shown that bioactive glass (BG) can enhance the formation of hydroxyapatite under simulated body fluid (SBF) conditions when combined with mineral trioxide aggregate (MTA). This study aims to assess the impact of BG-supplemented MTA on the biocompatibility and mineralization potential of dental pulp stem cells (DPSCs). <b>Methods:</b> We prepared ProRoot MTA (MTA) and MTA supplemented with 2% and 4% BG. Five passages of DPSCs were utilized for the experiments. The DPSCs were subjected to various tests to determine their morphology, viability, cell migration, and adhesion assay. Additionally, mineralization ability was assessed through SBF immersion treatment, alkaline phosphatase (ALP) activity test, Alizarin red S (ARS) staining, and real-time quantitative polymerase chain reaction (RT-qPCR) analysis. <b>Results:</b> The biocompatibility of BG-supplemented MTA was found to be comparable to that of conventional MTA, as demonstrated by the cell counting kit-8 (CCK-8) assay, cell migration, adhesion assays, and cell morphology on cement surfaces. Under SBF treatment, MTA supplemented with BG, particularly at a concentration of 4%, exhibited higher mineralization potential than conventional MTA in the ALP activity assay. This was supported by denser ARS staining, increased ALP activity, and higher expression of dentin sialophosphoprotein (DSPP), ALP, and bone morphogenetic protein-2 (BMP-2) in the SBF-treated MTABG group. <b>Conclusion:</b> Our study revealed that the biocompatibility of BG-supplemented MTA is similar to that of conventional MTA. Additionally, under SBF treatment, BG-supplemented MTA displayed enhanced mineralization potential, indicating that BG supplementation can augment the mineralization capabilities of MTA.</p>","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0142"},"PeriodicalIF":8.1,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11803057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regeneration of a Rabbit Segmental Defect with a New Bone Therapy: Autologous Blood Coagulum with Bone Morphogenetic Protein 6 and Synthetic Ceramics.","authors":"Nikola Stokovic, Natalia Ivanjko, Ana Javor, Marko Pecin, Katarina Muzina, Zeljka Magdalena Stepanic, Hrvoje Capak, Zoran Vrbanac, Drazen Maticic, Slobodan Vukicevic","doi":"10.34133/bmr.0140","DOIUrl":"10.34133/bmr.0140","url":null,"abstract":"<p><p>Segmental defects of long bones are among the most challenging and debilitating conditions in clinical medicine. Osteogrow-C is a novel osteoinductive device composed of recombinant human bone morphogenetic protein 6 (rhBMP6) delivered within autologous blood coagulum (ABC) with calcium phosphate ceramics that was proven efficacious in preclinical models of spinal fusion. This study aimed to evaluate the efficacy of Osteogrow-C in comparison to that of other osteoinductive therapies in a clinically relevant segmental defect model in rabbits. Segmental defects (15 mm) of rabbit ulna were treated with Osteogrow-C containing different synthetic ceramic particles (tricalcium phosphate [TCP] and TCP/hydroxyapatite 40%/60%), Osteogrow (rhBMP6/ABC), Infuse (rhBMP2/absorbable collagen sponge), and control implants without bone morphogenetic proteins. Defect healing was evaluated by in vivo x-ray scans 4, 8, and 17 weeks after the surgery, and animals were killed after 17 weeks for further radiographical and histological assessment. Evaluation of x-ray images, micro-computed tomography, and histological sections revealed that both Osteogrow-C formulations as well as Osteogrow and Infuse promoted healing of the ulnar segmental defect. However, radiographic scores were higher in animals treated with Osteogrow-C than those for the other used therapies. Moreover, evaluation of in vivo x-ray scans revealed that Osteogrow-C with TCP ceramics induced the most rapid defect bridging. On the other hand, control implants (ABC/TCP and ABC/biphasic calcium phosphate) promoted limited osteogenesis without defect bridging. The findings of this study suggest that Osteogrow-C is a promising safe therapeutic solution for the treatment of large bone defects, providing relief to millions of patients suffering from this debilitating condition.</p>","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0140"},"PeriodicalIF":8.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11794767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}