Biomaterials research最新文献

Autophagy-Regulating, Photothermal Polydopamine-Coated, and Photodynamic Zirconium/Porphyrin-Framed Metal-Organic Frameworks for Enhanced Doxorubicin Therapy in Colon Cancer. 自噬调节、光热聚多巴胺包被和光动力锆/卟啉框架金属有机框架增强阿霉素治疗结肠癌。

IF 8.1

Biomaterials research Pub Date : 2025-06-12 eCollection Date: 2025-01-01 DOI: 10.34133/bmr.0218

Junghan Lee, Kwangsun Yu, Enkhzaya Davaa, Ratchapol Jenjob, Phuong Hoa Tran, Dahee Ryu, Jongyoon Kim, Seongju Lee, Zheyu Shen, Wha-Seung Ahn, Chung-Sung Lee, Su-Geun Yang

{"title":"Autophagy-Regulating, Photothermal Polydopamine-Coated, and Photodynamic Zirconium/Porphyrin-Framed Metal-Organic Frameworks for Enhanced Doxorubicin Therapy in Colon Cancer.","authors":"Junghan Lee, Kwangsun Yu, Enkhzaya Davaa, Ratchapol Jenjob, Phuong Hoa Tran, Dahee Ryu, Jongyoon Kim, Seongju Lee, Zheyu Shen, Wha-Seung Ahn, Chung-Sung Lee, Su-Geun Yang","doi":"10.34133/bmr.0218","DOIUrl":"10.34133/bmr.0218","url":null,"abstract":"Metal-organic frameworks (MOFs) have immense potential for biomedical applications. This paper reports the development of multifunctional zirconium-based metal-organic framework (ZrMOF) nanohybrids, featuring a photodynamic porphyrin-framed zirconium cluster with photothermal polydopamine (PD) coating. The PD-coated ZrMOF (PD/ZrMOF) nanohybrids exhibit enhanced colloidal stability and biocompatibility. The PD/ZrMOF nanohybrids in the present study exhibited a unique combination of functionalities, including photodynamic therapy (PDT), photothermal therapy (PTT), and the delivery of anticancer agents. Furthermore, hydrazone-modified doxorubicin (DOX-hyd) was encapsulated within the PD/ZrMOF nanohybrids, enabling a pH-responsive release mechanism that responds to acidic conditions within the tumor microenvironment. This study examined how MOFs influence autophagy, which is essential for maintaining cellular homeostasis in various human diseases, resulting in autophagy activation by MOF treatment. Additional research into the possible mechanisms of autophagy by MOF showed that the up-regulation of Beclin-1 and ATG7, independent of the mTOR pathway, contributes to autophagy induction. Furthermore, the DOX-hyd-encapsulated PD/ZrMOF nanohybrids (DOX-hyd-PD/ZrMOF) exhibited remarkable cancer suppression ability in vitro and in vivo, owing to their tri-mode therapeutic capabilities comprising PDT, PTT, and chemotherapy. This versatile \"three-in-one\" nanoplatform enables efficient cancer imaging and offers a powerful strategy for multi-mode combination treatments.","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0218"},"PeriodicalIF":8.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12160315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144287474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanomaterials for Combating Cancer while Safeguarding Organs: Safe and Effective Integrative Tumor Therapy. 抗癌护器官的纳米材料：安全有效的肿瘤综合治疗。

IF 8.1

Biomaterials research Pub Date : 2025-06-12 eCollection Date: 2025-01-01 DOI: 10.34133/bmr.0165

Keqin Ji, Xianghe Jiang, Zhuanzhuan Zhang, Mengfan Li, Zhu Peng, Yao Wang, Jie Gao

{"title":"Nanomaterials for Combating Cancer while Safeguarding Organs: Safe and Effective Integrative Tumor Therapy.","authors":"Keqin Ji, Xianghe Jiang, Zhuanzhuan Zhang, Mengfan Li, Zhu Peng, Yao Wang, Jie Gao","doi":"10.34133/bmr.0165","DOIUrl":"10.34133/bmr.0165","url":null,"abstract":"Cancer remains a leading cause of mortality globally. Combating cancer while safeguarding organs (CCSO) has emerged as a specialized field that employs a multifaceted approach to cancer management. Postsurgery solid tumors face issues such as recurrence and organ dysfunction due to residual cancer, resection, inflammation, and infections. Adjuvant and preventive treatments may also impair organ function, adding to treatment challenges. This review delineates the multifaceted landscape of multidimensional nanomaterials, spanning from 0-dimensional nanoparticles to 3-dimensional scaffolds, and their collaborative roles in concurrent cancer management and organ protection. We underscore the importance of nanomaterial synthesis, functionalization, and responsive release mechanisms in the tumor and organ microenvironments. A comprehensive analysis of nanomaterial applications in integrated cancer management, including melanoma, osteosarcoma, breast cancer, liver cancer, pancreatic cancer, and gastric cancer, is presented, highlighting their potential to overcome therapeutic challenges. The discourse also addresses the obstacles and future directions for nanomaterials for CCSO, offering valuable insights for advancing cancer management and organ protection. This review aims to enhance the comprehension and progress of nanomaterials for CCSO, fostering the development of more effective cancer management modalities.","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0165"},"PeriodicalIF":8.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144287475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ginsenoside Rh2-Pretreated Mesenchymal Stem Cell Exosomes Ameliorate Collagen-Induced Arthritis via N6-Methyladenosine Methylation. 人参皂苷rh2预处理间充质干细胞外泌体通过n6 -甲基腺苷甲基化改善胶原诱导的关节炎。

IF 8.1

Biomaterials research Pub Date : 2025-06-11 eCollection Date: 2025-01-01 DOI: 10.34133/bmr.0220

Zhongsheng Zhou, Shuhui Wu, Yang Li, Pu Shao, Jinlan Jiang

{"title":"Ginsenoside Rh2-Pretreated Mesenchymal Stem Cell Exosomes Ameliorate Collagen-Induced Arthritis via N6-Methyladenosine Methylation.","authors":"Zhongsheng Zhou, Shuhui Wu, Yang Li, Pu Shao, Jinlan Jiang","doi":"10.34133/bmr.0220","DOIUrl":"10.34133/bmr.0220","url":null,"abstract":"This research examines the impact of exosomes derived from mesenchymal stem cells that have been pretreated with ginsenoside Rh2 (Rh2-pre Exo) in the context of collagen-induced arthritis (CIA). Rheumatoid arthritis (RA) is a persistent inflammatory condition marked by joint pain and swelling, which, in advanced stages, may result in joint damage and reduced functionality. We found that Rh2-pro Exo regulates the Toll-like receptor 4 (TLR4)/Myd88/nuclear factor κB (NF-κB) signaling pathway by modulating the m6A methylation levels of C-C motif chemokine receptor like 2 (CCRL2). The interaction between CCRL2 and TLR4 is a key factor influencing the activity of this signaling pathway. Our results indicate that this regulatory mechanism enhances the anti-inflammatory phenotype of macrophages, promoting a shift from pro-inflammatory to anti-inflammatory responses. Furthermore, treatment with Rh2-pre Exo substantially alleviated clinical symptoms and reduced joint damage in CIA models. These findings provide new insights into the therapeutic potential of Rh2-pre Exo in the treatment of RA, emphasizing the importance of m6A methylation in regulating immune responses. This study suggests that targeting the m6A methylation pathway of CCRL2 could offer a promising strategy for developing effective therapies for RA, ultimately improving patient outcomes and quality of life.","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0220"},"PeriodicalIF":8.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144277043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumoricidal Efficacy of Artesunate-Eluting Microsphere: Differential Role of Bax/Bak in Orchestration of Cell Death Pathways. 青蒿琥酯洗脱微球的杀瘤效果：Bax/Bak在细胞死亡通路编排中的不同作用。

IF 8.1

Biomaterials research Pub Date : 2025-06-10 eCollection Date: 2025-01-01 DOI: 10.34133/bmr.0217

Sarah Helmueller, Sanghee Lee, Xinxin Song, Dong-Hyun Kim, Yong J Lee

{"title":"Tumoricidal Efficacy of Artesunate-Eluting Microsphere: Differential Role of Bax/Bak in Orchestration of Cell Death Pathways.","authors":"Sarah Helmueller, Sanghee Lee, Xinxin Song, Dong-Hyun Kim, Yong J Lee","doi":"10.34133/bmr.0217","DOIUrl":"10.34133/bmr.0217","url":null,"abstract":"Artesunate (ART), an antimalarial drug, has been identified as a ferroptotic agent, inducing the generation of reactive oxygen species (ROS) and lipid peroxidation, which, in turn, activate endoplasmic reticulum (ER) stress responses and promote mitochondrial-dependent apoptosis. In our previous studies, we demonstrated that ART enhances tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis through crosstalk between the ER stress-mediated signal pathway and the Bid-Bax mitochondrial apoptotic cascade. To further explore the mechanisms underlying ferroptotic-apoptotic crosstalk and evaluate the potential of intra-arterial drug-eluting microspheres for targeted tumor therapy, we developed artesunate-eluting microspheres (ART-EMs) and investigated the tumoricidal efficacy of ART-EMs combined with TRAIL. Our findings reveal that the combined ART-EMs with TRAIL (AT) treatment synergistically enhances cancer cell death. Specifically, we observed increased apoptosis in HCT116 and BxPC-3 cell lines, accompanied by notable morphological changes and enhanced cytotoxicity. Importantly, our results demonstrate that the pro-apoptotic proteins Bid and Bax play essential roles in driving synergistic apoptosis during AT treatment. Furthermore, the contrasting apoptotic responses between AT treatment and the chemotherapeutic agent mitomycin C's dependence on p53-Bak-associated pathways underscore the differential activation of intrinsic apoptosis pathways across cancer cell lines. This study provides deeper insight into the roles of Bak and Bax in orchestrating apoptosis, offering potential strategies for more effective cancer treatments.","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0217"},"PeriodicalIF":8.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144268162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polydopamine/Montmorillonite-Based Brushite Calcium Phosphate Cements: Synergize Mechanical and Osteogenic Capacity for Bone Regeneration. 聚多巴胺/蒙脱石基刷石磷酸钙水泥：骨再生的协同机械和成骨能力。

IF 8.1

Biomaterials research Pub Date : 2025-06-09 eCollection Date: 2025-01-01 DOI: 10.34133/bmr.0213

Yukang Gong, Yanan Niu, Chenhao Wang, Gaoqi Ye, Menghang Chen, Gaokai Hu, Yang Hu, Yuhan Xue, Tao Guo, Wenjuan Yin, Yuangong Zhang, Zheng Gao, Feng Liu, Wenshan Gao

{"title":"Polydopamine/Montmorillonite-Based Brushite Calcium Phosphate Cements: Synergize Mechanical and Osteogenic Capacity for Bone Regeneration.","authors":"Yukang Gong, Yanan Niu, Chenhao Wang, Gaoqi Ye, Menghang Chen, Gaokai Hu, Yang Hu, Yuhan Xue, Tao Guo, Wenjuan Yin, Yuangong Zhang, Zheng Gao, Feng Liu, Wenshan Gao","doi":"10.34133/bmr.0213","DOIUrl":"10.34133/bmr.0213","url":null,"abstract":"Although brushite calcium phosphate cements (Bru-CPCs) possess good bioactivity and biocompatibility, their low compressive strength hinders their effective application in osteoporotic bone defect treatment. Therefore, the aim of this study was to improve the compressive strength without sacrificing the osteogenesis properties of Bru-CPCs. Montmorillonite (MMT) was added into Bru-CPCs to get Bru-CPCs/1.5% MMT. The compressive strength of Bru-CPCs/1.5% MMT increased to 13.31 MPa, but the interfacial interactions between MMT and Bru-CPCs (inorganic-inorganic phase) limited further improvement in compressive strength. Inspired by the adhesive proteins in mussels, MMT was coated with a polydopamine (PDA) layer to get MMT@PDA. The compressive strength of Bru-CPCs/1.5% MMT@PDA further increased to 16.58 MPa. Then, the surface morphology, adhesiveness, biocompatibility, and bioactivity of CPCs/1.5% MMT@PDA were evaluated. All data indicated that by adding MMT@PDA, the compressive strength was improved without sacrificing the osteogenesis properties of Bru-CPCs.","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0213"},"PeriodicalIF":8.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144259563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-Active Nanoimmunomodulators for the Synergistic Enhancement of the Antitumor Efficacy of Photodynamic Immunotherapy. 双活性纳米免疫调节剂协同增强光动力免疫治疗的抗肿瘤效果。

IF 8.1

Biomaterials research Pub Date : 2025-06-09 eCollection Date: 2025-01-01 DOI: 10.34133/bmr.0214

Ping Dong, Shaowen Zhang, Ying Zhang, Haifeng Hu, Qing Zhou, Yanzhuo Liu, Zhangfan Mao

{"title":"Dual-Active Nanoimmunomodulators for the Synergistic Enhancement of the Antitumor Efficacy of Photodynamic Immunotherapy.","authors":"Ping Dong, Shaowen Zhang, Ying Zhang, Haifeng Hu, Qing Zhou, Yanzhuo Liu, Zhangfan Mao","doi":"10.34133/bmr.0214","DOIUrl":"10.34133/bmr.0214","url":null,"abstract":"Photodynamic immunotherapy, which combines photodynamic therapy (PDT) with immunotherapy, has become an important and effective treatment for cancer. However, most photodynamic immunotherapy systems for cancer do not allow for the precise release of immunomodulators, leading to systemic side effects and poor patient prognosis. This study reports a dual-activatable nanoimmunomodulator (CPPM), whose photodynamic effect and agonist release are both activated in response to specific stimuli, which can be used for precise photodynamic immunotherapy of cancer. CPPM has a half-life of 119 min in circulation and accumulates in tumor tissue 4 h after injection (23.8%). In addition, CPPM is able to achieve tumor localization of nanomedicines through PD-L1-targeting peptides, blocking the specific binding of PD-L1 to PD-1, exposing tumor surface antigens, and reinvigorating the activity of T cells in combination with macitentan to promote T-cell proliferation. Meanwhile, under laser irradiation, CPPM was able to increase intracellular oxidative stress, inhibit cell proliferation through PDT, and trigger immunogenic cell death, further enhancing tumor immunogenicity through synergistic treatment. Ultimately, CPPM enhanced the immunotherapeutic efficiency against tumors by improving the tumor immunosuppressive microenvironment, synergistically inhibiting the growth of primary and distant tumors while activating systemic antitumor immunity to eliminate lung metastases without obvious side effects. This study presents an uncomplicated and multifunctional strategy for the precise modulation of tumor photodynamic immunotherapy with a dual-activatable smart nanoimmunomodulator that can improve the efficacy of PDT, enhance systemic antitumor immunity, and potentially extend it to a wide range of cancers.","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0214"},"PeriodicalIF":8.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144259562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Icariin-Releasing 3-Dimensionally Printed Scaffolds for Alveolar Cleft Reconstruction. 释放淫羊藿素的三维打印牙槽裂重建支架。

IF 8.1

Biomaterials research Pub Date : 2025-05-30 eCollection Date: 2025-01-01 DOI: 10.34133/bmr.0199

Soomin Park, Alexander B Pascal, Sidney B Eisig, Meng Feng, Hun Jin Jeong, Elen Zhu, Emily Zhang, Chang Hun Lee

{"title":"Icariin-Releasing 3-Dimensionally Printed Scaffolds for Alveolar Cleft Reconstruction.","authors":"Soomin Park, Alexander B Pascal, Sidney B Eisig, Meng Feng, Hun Jin Jeong, Elen Zhu, Emily Zhang, Chang Hun Lee","doi":"10.34133/bmr.0199","DOIUrl":"10.34133/bmr.0199","url":null,"abstract":"Each year, 1 in every 700 babies is born with an orofacial cleft in the USA. Despite a well-established protocol for early cleft repair, the alveolar cleft persists during craniofacial growth. Current surgical treatments with bone grafts for alveolar cleft often provide inadequate nasal base support and insufficient alveolar bone volume for permanent tooth eruption. Here, we developed 3-dimensionally printed polycaprolactone scaffolds with controlled delivery of icariin (ICA) to facilitate bone reconstruction. After establishing a reliable fabrication process, we determined the optimal loading dose and release kinetics of ICA for induced osteogenic differentiation of bone marrow mesenchymal stem/progenitor cells and mineralized tissue formation in vitro. Then, the ICA-releasing polycaprolactone scaffolds with the preoptimized dose were implanted into rats with full-thickness maxillary defects. Up to 8 weeks, micro-computed tomography analyses demonstrated significantly accelerated bone healing and defect closure with an ICA-releasing scaffold compared to scaffold alone and defect controls. Histology consistently confirmed the formation of dense woven bone with ICA-releasing scaffolds in contrast to unclosed gaps and soft tissue infiltration in controls. Our findings suggest the significant potential of ICA-releasing 3-dimensionally printed scaffolds to serve as a patient-focused and custom-built bone graft to improve the clinical outcome of alveolar cleft reconstruction.","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0199"},"PeriodicalIF":8.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144201046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomimetic Microchannel Integrated Silk Fibroin Scaffold for Regeneration of Intervertebral Disc Degeneration. 仿生微通道集成丝素蛋白支架用于椎间盘退变的再生。

IF 8.1

Biomaterials research Pub Date : 2025-05-28 eCollection Date: 2025-01-01 DOI: 10.34133/bmr.0203

Tongxing Zhang, Zhaojun Cheng, Zhen Zhang, Lilong Du, Zhenhua Li, Zhuyan Jiang, Zhaomin Zheng, Deling Kong, Meifeng Zhu, Wen Li, Baoshan Xu

{"title":"Biomimetic Microchannel Integrated Silk Fibroin Scaffold for Regeneration of Intervertebral Disc Degeneration.","authors":"Tongxing Zhang, Zhaojun Cheng, Zhen Zhang, Lilong Du, Zhenhua Li, Zhuyan Jiang, Zhaomin Zheng, Deling Kong, Meifeng Zhu, Wen Li, Baoshan Xu","doi":"10.34133/bmr.0203","DOIUrl":"10.34133/bmr.0203","url":null,"abstract":"Intervertebral disc degeneration (IVDD) is the primary cause of low back pain, and patients with severe degeneration usually require lumbar fusion or total disc arthroplasty. Lumbar fusion carries the risk of accelerated degeneration of the adjacent intervertebral disc (IVD), and total disc arthroplasty could reduce the risk. However, the clinical application of artificial IVD whose nondegradable properties make it difficult to restore the biological function of the IVD. Therefore, we intend to fabricate a novel biomimetic microchannel integrated silk fibroin scaffold (BMI-SF scaffold) containing annulus fibrosus with oriented cross-microchannels and nucleus pulposus with interconnected porous structure. The BMI-SF scaffold exhibits controllable microchannels as well as excellent biocompatibility and biodegradability. In vitro and in vivo studies have demonstrated that microchannels can direct cells into the BMI-SF scaffold and enhance neovascularization, supplying adequate nutritional support for tissue regeneration. The IVD replacement model showed that the BMI-SF scaffold has superior regenerative effects, such as restoring IVD height and providing motion segments with dynamic mechanical properties akin to the natural IVD. In this study, the BMI-SF scaffold developed using controlled microchannels provides a new strategy for patients with severe IVDD and has broad clinical application prospects.","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0203"},"PeriodicalIF":8.1,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-Free Prediction of Fluorescently Labeled Fibrin Networks. 荧光标记纤维蛋白网络的无标记预测。

IF 8.1

Biomaterials research Pub Date : 2025-05-28 eCollection Date: 2025-01-01 DOI: 10.34133/bmr.0211

Sarah Eldeen, Andres Felipe Guerrero Ramirez, Bora Keresteci, Peter D Chang, Elliot L Botvinick

{"title":"Label-Free Prediction of Fluorescently Labeled Fibrin Networks.","authors":"Sarah Eldeen, Andres Felipe Guerrero Ramirez, Bora Keresteci, Peter D Chang, Elliot L Botvinick","doi":"10.34133/bmr.0211","DOIUrl":"10.34133/bmr.0211","url":null,"abstract":"While fluorescent labeling has been the standard for visualizing fibers within fibrillar scaffold models of the extracellular matrix (ECM), the use of fluorescent dyes can compromise cell viability and photobleach prematurely. The intricate fibrillar composition of ECM is crucial for its viscoelastic properties, which regulate intracellular signaling and provide structural support for cells. Naturally derived biomaterials such as fibrin and collagen replicate these fibrillar structures, but longitudinal confocal imaging of fibers using fluorescent dyes may impact cell function and photobleach the sample long before termination of the experiment. An alternative technique is reflection confocal microscopy (RCM) that provides high-resolution images of fibers. However, RCM is sensitive to fiber orientation relative to the optical axis, and consequently, many fibers are not detected. We aim to recover these fibers. Here, we propose a deep learning tool for predicting fluorescently labeled optical sections from unlabeled image stacks. Specifically, our model is conditioned to reproduce fluorescent labeling using RCM images at 3 laser wavelengths and a single laser transmission image. The model is implemented using a fully convolutional image-to-image mapping architecture with a hybrid loss function that includes both low-dimensional statistical and high-dimensional structural components. Upon convergence, the proposed method accurately recovers 3-dimensional fibrous architecture without substantial differences in fiber length or fiber count. However, the predicted fibers were slightly wider than original fluorescent labels (0.213 ± 0.009 μm). The model can be implemented on any commercial laser scanning microscope, providing wide use in the study of ECM biology.","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0211"},"PeriodicalIF":8.1,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing the Surface Functionalization of Peptide-MXene Nanoplatforms to Amplify Tumor-Targeting Efficiency and Photothermal Therapy. 优化肽- mxene纳米平台的表面功能化以增强肿瘤靶向效率和光热治疗。

IF 8.1

Biomaterials research Pub Date : 2025-05-26 eCollection Date: 2025-01-01 DOI: 10.34133/bmr.0198

Sujin Kim, Sathiyamoorthy Padmanaban, Aravindkumar Sundaram, Gul Karima, In-Kyu Park, Hwan D Kim

{"title":"Optimizing the Surface Functionalization of Peptide-MXene Nanoplatforms to Amplify Tumor-Targeting Efficiency and Photothermal Therapy.","authors":"Sujin Kim, Sathiyamoorthy Padmanaban, Aravindkumar Sundaram, Gul Karima, In-Kyu Park, Hwan D Kim","doi":"10.34133/bmr.0198","DOIUrl":"10.34133/bmr.0198","url":null,"abstract":"Energy storage and conversion extensively use MXenes, a class of 2-dimensional transition metals. Research is currently exploring MXenes in areas such as biomedical imaging, positioning them as a substantial contender in biomedical applications. Even though these biocompatible MXenes have many uses, it is challenging to make nanoparticles that are all the same size. This has made it harder to use them in the biomedical field. Herein, we meticulously crafted nano-sized MXene particles, achieving exceptional uniformity and amplified photothermal conversion efficiency compared to those of their bulkier micro-sized counterparts. To make these nanoparticles better at finding tumors, we added ARGD peptides to their surfaces. These are biomolecules that are known to bind to integrin αvβ3, a protein that is highly expressed in cancerous cells. Our research showed that these RGD-MXene nanoconjugates have excellent targeting accuracy and can eradicate tumors very effectively. This targeted photothermal therapy platform promises to redefine cancer treatment by selectively eradicating malignant cells while safeguarding healthy tissue. Also, MXene's natural ability to change surfaces opens up a world of possibilities for a wide range of uses in nanomedicine, bringing about a new era of sophisticated therapeutic interventions.","PeriodicalId":93902,"journal":{"name":"Biomaterials research","volume":"29 ","pages":"0198"},"PeriodicalIF":8.1,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144153106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0