Asian journal of andrology最新文献_第2页

Retraction note: The calcium-sensing receptor participates in testicular damage in streptozotocin-induced diabetic rats. 撤回注：钙敏感受体参与链脲佐菌素诱导的糖尿病大鼠睾丸损伤。

IF 2.7

Asian journal of andrology Pub Date : 2026-05-01 Epub Date: 2026-03-06 DOI: 10.4103/aja2025121

Wei-Yuan Kong, Li-Quan Tong, Hai-Jun Zhang, Yong-Gang Cao, Gong-Chen Wang, Jin-Zhi Zhu, Feng Zhang, Xue-Ying Sun, Tie-Hui Zhang, Lin-Lin Zhang

引用次数: 0

Clinical features and genetic analysis of androgen receptor gene variants in 30 prepubertal patients with androgen insensitivity syndrome. 30例青春期前雄激素不敏感综合征患者雄激素受体基因变异的临床特征及遗传分析。

IF 2.7

Asian journal of andrology Pub Date : 2026-05-01 Epub Date: 2026-01-20 DOI: 10.4103/aja202578

Jia-Nan Li, Jiang Wei, Jing Hui, Zi-Xuan Wang, Ji-Yun Yang

{"title":"Clinical features and genetic analysis of androgen receptor gene variants in 30 prepubertal patients with androgen insensitivity syndrome.","authors":"Jia-Nan Li, Jiang Wei, Jing Hui, Zi-Xuan Wang, Ji-Yun Yang","doi":"10.4103/aja202578","DOIUrl":"10.4103/aja202578","url":null,"abstract":"Androgen insensitivity syndrome (AIS; Online Mendelian Inheritance in Man [OMIM; #300068]) is an X-linked recessive disorder caused by pathogenic variants in the androgen receptor (AR) gene located in the Xq11-q13 region. In this retrospective study of 30 patients with AIS, next-generation sequencing identified 24 variants in AR, including 20 missense, 1 nonsense, and 3 splice-site variants. Seven novel variants were detected in 8 patients. Of the 24 variants, 15 were classified as likely pathogenic, 8 as pathogenic, and 1 as of uncertain significance. Variants included 5 de novo and 24 familial cases. These AR variants were predominantly located in the functional domains, with the ligand-binding domain (LBD) harboring 10 variants, the DNA-binding domain (DBD) harboring 5 variants, the N-terminal domain (NTD) harboring 2 variants, and the hinge region (HR) harboring 1 variant. The highest variant detection rate occurred in exon 5 (11/30), followed by exon 3 (9/30). These findings advance our understanding of genotype including 20 missense, 1 nonsense, and 3 splice-site variants through the identification of 7 novel variants.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"335-342"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146013903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fresh or frozen testicular sperm? Guidance from an etiology-classified retrospective study on ICSI cycles for patients with azoospermia. 新鲜的还是冷冻的睾丸精子？无精子症患者ICSI周期病因分类回顾性研究的指导。

IF 2.7

Asian journal of andrology Pub Date : 2026-05-01 DOI: 10.4103/aja2025120

Zi-Jue Zhu, Liang-Hong Ma, Cun-Zhong Deng, Fu-Rong Bai, Peng Li, Li-Na Zhou, Shan Jiang, Xiao-Jin He, Zheng Li, Wei Chen

{"title":"Fresh or frozen testicular sperm? Guidance from an etiology-classified retrospective study on ICSI cycles for patients with azoospermia.","authors":"Zi-Jue Zhu, Liang-Hong Ma, Cun-Zhong Deng, Fu-Rong Bai, Peng Li, Li-Na Zhou, Shan Jiang, Xiao-Jin He, Zheng Li, Wei Chen","doi":"10.4103/aja2025120","DOIUrl":"https://doi.org/10.4103/aja2025120","url":null,"abstract":"Abstract: Using testicular sperm, men with azoospermia can father children. Sperm cryopreservation helps avoid the risk of cycle canceling because of the failure of testicular sperm retrieval. However, cryopreservation of testicular sperm can potentially impair sperm function. In addition, the different qualities of testicular sperm retrieved from men with various etiologies of azoospermia can lead to different intracytoplasmic sperm injection (ICSI) outcomes after sperm cryopreservation. A retrospective analysis was performed to elucidate the influence of frozen testicular sperm from men with different etiologies of azoospermia on assisted reproductive technology (ART) treatment outcomes. Testicular sperm retrieval rates were also compared. The fertilization rate was found to be influenced by testicular sperm cryopreservation in most cases, especially for patients with azoospermia factor c (AZFc) deletion, and embryo development was significantly impeded when frozen testicular sperm from Klinefelter syndrome (KS) patients were used. Moreover, ART treatment outcomes after embryo transfer were not significantly influenced by testicular sperm cryopreservation for any of the etiologies. In addition, significantly higher sperm retrieval rates were achieved in cryptorchidism, KS, and AZFc deletion patients, whereas the testicular sperm retrieval rate was significantly lower in patients with idiopathic nonobstructive azoospermia. Collectively, our findings suggest that except for patients with KS, testicular sperm retrieval prior to initiating ovarian stimulation of the patient's partner should be considered the first-line approach. In addition, assisted oocyte activation should be performed in ICSI cycles using frozen testicular sperm, especially for patients with AZFc deletion.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147847341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of individualized surgical strategies for pediatric hypospadias: a multicenter penile morphometric analysis. 儿童尿道下裂个体化手术策略的发展：多中心阴茎形态计量学分析。

IF 2.7

Asian journal of andrology Pub Date : 2026-05-01 Epub Date: 2026-01-13 DOI: 10.4103/aja202571

Yi-Wei Fang, Hong-Cheng Song, Yun-Man Tang, Lu-Gang Huang, Yi Yang, Min Chao, Hong Ma, Jing-Ti Zhang, Xu-Hui Zhang, Shou-Lin Li, Ning Li, Chao Chen, Da-Wei He, Wen-Bo Wu, Hua Xie, Yong Guan, Yan-Fang Yang, Jian-Guo Zhang

{"title":"Development of individualized surgical strategies for pediatric hypospadias: a multicenter penile morphometric analysis.","authors":"Yi-Wei Fang, Hong-Cheng Song, Yun-Man Tang, Lu-Gang Huang, Yi Yang, Min Chao, Hong Ma, Jing-Ti Zhang, Xu-Hui Zhang, Shou-Lin Li, Ning Li, Chao Chen, Da-Wei He, Wen-Bo Wu, Hua Xie, Yong Guan, Yan-Fang Yang, Jian-Guo Zhang","doi":"10.4103/aja202571","DOIUrl":"10.4103/aja202571","url":null,"abstract":"This multicenter study aimed to establish a quantitative, individualized surgical decision algorithm for pediatric hypospadias by analyzing multicenter penile anatomical data, surgical approaches, and follow-up outcomes. To achieve this purpose, clinical data from 1500 primary hypospadias cases across 17 tertiary centers in China (December 2018 to September 2021) were retrospectively reviewed, with patients stratified into urethral plate preservation group ( n = 715) and transection group ( n = 785). Using multivariate logistic regression, key predictors for intraoperative urethral plate transection were identified, and morphometric parameters were analyzed to guide surgical selection. This analysis led to the development of a predictive nomogram and risk stratification thresholds, which were subsequently validated. The results demonstrated that significant predictors of transection included glans length (odds ratio [OR]: 1.17, 95% confidence interval [CI]: 1.07-1.29), glans width (OR: 0.35, 95% CI: 0.29-0.43), and penile curvature (OR: 1.07, 95% CI: 1.06-1.08), with the nomogram showing excellent discrimination (area under the receiver operating characteristic curve [AUC]: 0.914 in training and 0.87 in validation). Furthermore, for urethral plate preservation, a urethral plate width threshold of 4.25 mm differentiated optimal candidates for tubularized incised plate urethroplasty (width ≥4.25 mm) versus onlay island flap (width <4.25 mm). Notably, in transected cases, a urethral defect length of >3.55 cm was associated with higher complication rates in single-stage repairs (45.8% vs 33.4%, P < 0.05), favoring staged approaches. This algorithm integrates preoperative morphometrics and intraoperative measurements to provide objective, quantifiable guidance for individualized surgical planning, particularly benefiting less experienced surgeons.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"259-266"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145960951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional and epigenetic changes associated with lead exposure in spermatozoa. 精子中与铅暴露相关的转录和表观遗传变化。

IF 2.7

Asian journal of andrology Pub Date : 2026-05-01 Epub Date: 2026-01-13 DOI: 10.4103/aja202563

Xu Zhang, Xiao-Rong Shen, Bin Wu, Xue-Mei Wang, Hui-Juan Shi, Tian-Cheng Zhang

{"title":"Transcriptional and epigenetic changes associated with lead exposure in spermatozoa.","authors":"Xu Zhang, Xiao-Rong Shen, Bin Wu, Xue-Mei Wang, Hui-Juan Shi, Tian-Cheng Zhang","doi":"10.4103/aja202563","DOIUrl":"10.4103/aja202563","url":null,"abstract":"Lead (Pb) exposure is a risk factor of male infertility, while the transcriptional and epigenetic changes associated with lead exposure in spermatozoa are poorly understood. Our previous findings revealed significant changes in DNA methylation of the calcium (Ca) homeostasis pathway of human spermatozoa in men with a blood Pb level over 5 µg dl -1 , which was associated with decreased sperm motility. In this study, we explored the effects of Pb exposure on expression of differentially methylated genes (DMGs) by analyzing semen samples from six healthy, non-smoking, and non-drinking men (aged 20-40 years). Using methylated DNA immunoprecipitation sequencing (MeDIP-seq) and RNA sequencing (RNA-seq), we compared DNA methylation and RNA abundance patterns between two groups: three men with blood Pb level 0-2.5 µg dl -1 and three men with blood Pb level 5-10 µg dl -1 . Additionally, we experimentally validated the regulatory function of the differentially methylated regions associated with 11 hub genes using dual-luciferase reporter assays. We revealed differences in promoter activity between methylated and unmethylated promoter regions of seven cloned genes, namely calcium voltage-gated channel subunit alpha1 H ( CACNA1H ), calcium voltage-gated channel subunit alpha1 G ( CACNA1G ), calcium voltage-gated channel subunit alpha1 I ( CACNA1I ), calcium/calmodulin dependent protein kinase II gamma ( CAMK2G ), ATPase sarcoplasmic/endoplasmic reticulum Ca² + transporting 3 ( ATP2A3 ), solute carrier family 8 member A2 ( SLC8A2 ), and glutamate ionotropic receptor NMDA type subunit 2D ( GRIN2D ). Our results of Pb exposure-induced expression changes of essential genes associated with the calcium signaling pathway, particularly CACNA1H , SLC8A2, and GRIN2D , in spermatozoa, may be a potential cause of low sperm quality.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"297-303"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145961083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SPACA4 regulates the structure and molecular basis of spermatid maturation and ultimately affects sperm quality in mice. SPACA4调节精子成熟的结构和分子基础，最终影响小鼠精子质量。

IF 2.7

Asian journal of andrology Pub Date : 2026-05-01 Epub Date: 2026-01-13 DOI: 10.4103/aja202553

Xu Chen, Hai-Qian Wu, Dan-Yang Wan, Xiang-Zheng Zhang, Xin-Li Zhou, Xue-Jiang Guo, Hui Zhu

{"title":"SPACA4 regulates the structure and molecular basis of spermatid maturation and ultimately affects sperm quality in mice.","authors":"Xu Chen, Hai-Qian Wu, Dan-Yang Wan, Xiang-Zheng Zhang, Xin-Li Zhou, Xue-Jiang Guo, Hui Zhu","doi":"10.4103/aja202553","DOIUrl":"10.4103/aja202553","url":null,"abstract":"Sperm quality is crucial for sperm function and can even affect embryo quality and offspring health. Spermatid maturation is extremely complex, as spermatids undergo morphological changes, laying the foundation for the execution of sperm function. The function of sperm acrosome-associated 4 (SPACA4) in spermatogenesis is not well known. The present study revealed that SPACA4 was specifically expressed in the acrosomes and cytoplasm of mouse spermatids. Spaca4 knockout mice demonstrated that the loss of SPACA4 led to male subfertility. The quality of mature sperm was abnormal in Spaca4 -/- mice, manifested by decreased motility and multiple deformities. Spaca4 -/- sperm exhibited irregular nuclear shapes, abnormal nuclei with vacuoles, missing or incompletely fused acrosomes, and multiple cross-sections enclosed in the same sperm cell membrane. Electron microscopy and molecular expression analyses of testicles revealed that the loss of SPACA4 affected the differentiation of the acrosome, acroplaxome, and manchette, resulting in abnormalities in nuclear elongation, chromatin condensation, and flagellar development. Interestingly, SPACA4 did not regulate spermiogenesis via the acetylcholine signaling pathway. Analysis of the differential protein expression profile revealed that the expression of 9 proteins was significantly decreased in Spaca4 -/- spermatids. A decreased protein, transformation-related protein 53 target 5 (TRP53TG5), was knocked down in spermatids and found that the phenotype was consistent with Spaca4 knockout mice. These results revealed that the absence of SPACA4 leads to abnormal spermatid maturation and affects sperm quality in mice. Abnormal sperm quality in Spaca4 -/- mice results in decreased sperm capacitation and a decreased acrosome response, ultimately affecting the fertility of male mice.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"310-320"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145961071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urinary isoflavone concentrations and semen parameters of Japanese men seeking fertility treatment. 寻求生育治疗的日本男性尿异黄酮浓度和精液参数。

IF 2.7

Asian journal of andrology Pub Date : 2026-05-01 Epub Date: 2026-01-13 DOI: 10.4103/aja202566

Shoko Konishi, Yuki Mizuno, Kazumitsu Yamasaki, Masahiro Uchida, Teruaki Iwamoto

{"title":"Urinary isoflavone concentrations and semen parameters of Japanese men seeking fertility treatment.","authors":"Shoko Konishi, Yuki Mizuno, Kazumitsu Yamasaki, Masahiro Uchida, Teruaki Iwamoto","doi":"10.4103/aja202566","DOIUrl":"10.4103/aja202566","url":null,"abstract":"This study aimed to analyze the associations between urinary genistein, daidzein, and equol concentrations and semen parameters in Japanese men exposed to high levels of isoflavone in their diet. Between September 2020 and March 2021, men seeking fertility treatment (n = 157) at Sanno Hospital (Tokyo, Japan) and Tsukuba Gakuen Hospital (Ibaraki, Japan) provided spot urine and semen specimens on the same day. Sperm concentration, sperm count, and total sperm motility were quantified. Urinary isoflavones were measured using liquid chromatography-tandem mass spectrometry and adjusted for specific gravity. The median (interquartile range [IQR]) total sperm count and concentration were 112 × 10 6 (50 × 10 6 , 221 × 10 6 ) and 39 × 10 6 (17 × 10 6 , 72 × 10 6 ) ml -1 , respectively. Men in the second, third, and fourth quartile of urinary daidzein concentration had -40% (95% confidence interval [CI]: -59%, -13%), -37% (95% CI: -56%, -9%), and -32% (95% CI: -53%, -3%) low sperm count, respectively, than those in the lowest quartile. Men in the second, third, and fourth quartile of urinary genistein concentration had -23% (95% CI: -47%, 12%), -50% (95% CI: -66%, -26%), and -29% (95% CI: -51%, 3%) low sperm count than those in the lowest quartile, respectively. Sperm count showed no association with urinary equol concentration ( P > 0.05). No associations were observed between urinary isoflavones and total sperm motility. A higher isoflavone intake may be associated with reduced sperm concentration and count. The effect of these alterations in semen parameters on the fecundity of couples trying to conceive remains unknown.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"252-258"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145961042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current risk factors for male infertility and semen parameters: an umbrella review of systematic reviews and meta-analyses. 当前男性不育的危险因素和精液参数：系统评价和荟萃分析的综合综述。

IF 2.7

Asian journal of andrology Pub Date : 2026-05-01 Epub Date: 2026-01-13 DOI: 10.4103/aja202552

Qi-Hao Wang, Jian-Jun Ye, Ze-Yu Chen, Chi-Chen Zhang, Xin-Yang Liao, Lei Zheng, Kai Chen, Xiang Tu, Liang-Ren Liu, Qiang Wei, Yi-Ge Bao

{"title":"Current risk factors for male infertility and semen parameters: an umbrella review of systematic reviews and meta-analyses.","authors":"Qi-Hao Wang, Jian-Jun Ye, Ze-Yu Chen, Chi-Chen Zhang, Xin-Yang Liao, Lei Zheng, Kai Chen, Xiang Tu, Liang-Ren Liu, Qiang Wei, Yi-Ge Bao","doi":"10.4103/aja202552","DOIUrl":"10.4103/aja202552","url":null,"abstract":"Male infertility poses a substantial healthcare challenge and severely impacts the lives of patients. We aimed to investigate the risk factors for infertility and abnormal semen parameters. We conducted a comprehensive search of the articles published in Web of Science, MEDLINE, and Embase databases from January 2000 to February 2025. Infertility, semen volume, sperm concentration, sperm count, sperm morphology, sperm motility, and sperm progressive motility were used as endpoints to evaluate the relevance of risk factors. A total of 43 studies were included, covering 67 risk factors associated with infertility and abnormal sperm parameters. A total of 249 effect sizes were scored individually using the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) tool, of which 136 (54.6%) were classified as \"very low\", 59 (23.7%) as \"low\", and 54 (21.7%) as \"moderate\". Suffering from type 1 diabetes, metabolic syndrome, hyperthyroidism, systemic lupus erythematosus, chronic prostatitis, and leukocytospermia may increase the risk of abnormal semen parameters. Poor lifestyle habits (obesity, sleep disorders, and smoking), exposure to pollutants and various compounds (carbon disulfide, organophosphates, and lead), the use of medications (sulfasalazine, mesalazine, and selective serotonin reuptake inhibitors), and even some viral infections (severe acute respiratory syndrome coronavirus 2, human papillomavirus, and hepatitis viruses) were associated with decreased semen quality. Regular physical exercise, nut consumption, and adherence to a healthy dietary pattern may reverse this process. An increasing number of factors are associated with infertility; however, some of the aforementioned studies lack verification of causal relationships. Future studies need to be well designed to further confirm these relationships.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"284-296"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145960978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rho-kinase inhibition reduces DNA fragmentation in human sperm cryopreservation. rho激酶抑制减少人类精子冷冻保存中的DNA断裂。

IF 2.7

Asian journal of andrology Pub Date : 2026-04-28 DOI: 10.4103/aja2025107

Pawan Limvorapitux, Isarin Thanaboonyawat, Japarath Prechapanich, Pitak Laokirkkiat, Roungsin Choavaratana, Somsin Petyim

{"title":"Rho-kinase inhibition reduces DNA fragmentation in human sperm cryopreservation.","authors":"Pawan Limvorapitux, Isarin Thanaboonyawat, Japarath Prechapanich, Pitak Laokirkkiat, Roungsin Choavaratana, Somsin Petyim","doi":"10.4103/aja2025107","DOIUrl":"https://doi.org/10.4103/aja2025107","url":null,"abstract":"Human sperm cryopreservation is essential for assisted reproductive technology; however, the freeze-thaw process reduces motility and increases DNA fragmentation. Rho-associated coiled-coil kinase (ROCK) inhibitors improve post-thaw survival in several cell types, yet their application to human sperm remains underexplored. We evaluated whether ROCK inhibition reduces DNA fragmentation during cryopreservation by liquid nitrogen vapor slow freezing or vitrification. Each semen sample was divided into four aliquots: 2 were cryopreserved via liquid nitrogen vapor slow freezing with or without ROCK inhibitor and 2 underwent vitrification with or without ROCK inhibitor. After 1 week of cryostorage, samples were thawed at the room temperature, and DNA fragmentation was quantified using the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. DNA fragmentation decreased with ROCK inhibitor versus control after liquid nitrogen vapor slow freezing (mean ± standard deviation [s.d.]: 10.47% ± 6.77% vs 13.54% ± 7.0%; P = 0.002) and after vitrification (mean ± s.d.: 12.51% ± 5.84% vs 16.5% ± 10.77%; P = 0.006). ROCK inhibition thus reduces post-thaw sperm DNA fragmentation with both cryopreservation methods, particularly with liquid nitrogen vapor slow freezing, supporting its potential to improve sperm storage for assisted reproductive technology.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147791815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Psychological and sexual associations of azoospermia in men: a cross-sectional pre-biopsy study. 男性无精子症的心理和性关联：一项横断面活检前研究。

IF 2.7

Asian journal of andrology Pub Date : 2026-04-28 DOI: 10.4103/aja2025116

Sule Yildirim Kopuk, Deniz Kulaksiz, Mehmet Yilmaz, Muhsin Balaban, Basar Ayribas, Asgar Garayev, Recep Burak Degirmentepe, Mehmet Ozay Ozgur, Eda Ozcan, Ahmet Guzel, Ismail Baglar, Tuncay Toprak

{"title":"Psychological and sexual associations of azoospermia in men: a cross-sectional pre-biopsy study.","authors":"Sule Yildirim Kopuk, Deniz Kulaksiz, Mehmet Yilmaz, Muhsin Balaban, Basar Ayribas, Asgar Garayev, Recep Burak Degirmentepe, Mehmet Ozay Ozgur, Eda Ozcan, Ahmet Guzel, Ismail Baglar, Tuncay Toprak","doi":"10.4103/aja2025116","DOIUrl":"https://doi.org/10.4103/aja2025116","url":null,"abstract":"Azoospermia can lead to significant psychological distress, low self-esteem, and compromised sexual function. However, the impact of these issues before any invasive diagnostic or treatment procedures is not well studied. We conducted a cross-sectional study involving 134 men diagnosed with azoospermia and 164 age-matched fertile controls. All assessments, including anxiety (Beck Anxiety Inventory [BAI]), depression (Beck Depression Inventory-II [BDI-II]), self-esteem (Rosenberg Self-Esteem Scale [RSES]), and erectile function (International Index of Erectile Function-5 [IIEF-5]), were conducted during the diagnosis visit, prior to any testicular biopsy for infertility treatment. Compared with age-matched fertile controls, men with azoospermia exhibited significantly higher anxiety levels (mean ± standard deviation [s.d.]: 17.3 ± 3.9 vs 9.8 ± 2.8), increased depression scores (mean ± s.d.: 24.1 ± 6.3 vs 12.5 ± 5.1), lower self-esteem (mean ± s.d.: 2.8 ± 0.5 vs 1.8 ± 0.3), and poorer erectile function (mean ± s.d.: 17.2 ± 5.1 vs 21.4 ± 5.2), all with P < 0.001. Importantly, decreased self-esteem was strongly correlated with both depression (r = 0.851) and anxiety (r = 0.843). Additionally, erectile dysfunction showed an inverse correlation with both depression (r = -0.867) and anxiety (r = -0.813), with P < 0.05 for both correlations. The psychological and sexual effects of infertility are often overlooked in the treatment process. Therefore, we advocate that all men with azoospermia receive psychological and sexual support alongside infertility treatment.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147791838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0