Asian journal of andrology最新文献_第2页

Genitourethral foreign bodies: 20-year experience and outcomes from a single center. 生殖泌尿道异物：一个中心 20 年的经验和结果。

Asian journal of andrology Pub Date : 2025-03-01 Epub Date: 2024-11-19 DOI: 10.4103/aja202497

Claris Oh, Darcy Noll, Athul John, Matthew Hong

{"title":"Genitourethral foreign bodies: 20-year experience and outcomes from a single center.","authors":"Claris Oh, Darcy Noll, Athul John, Matthew Hong","doi":"10.4103/aja202497","DOIUrl":"10.4103/aja202497","url":null,"abstract":"Abstract: The presentation of a foreign body in the lower urinary tract is a challenging urological emergency with highly variable management approaches. A retrospective cohort study was undertaken to identify the patients who presented with self-inserted genitourethral foreign body to adult tertiary centers within our local health network (Southern Adelaide Local Health Network, Adelaide, Australia) from October 2002 to October 2022. Patient demographics, type of foreign body, psychiatric comorbidities, retrieval techniques, complications, and readmission data were extracted. Twenty-seven cases were identified with a median age of 44 years. Twenty-three (85.2%) were male and 4 (14.8%) were female. Ten patients (37.0%) had a concurrent psychiatric illness. The most commonly inserted foreign bodies were plastic tubes/wires (29.6%). Twenty cases (74.1%) were retrieved endoscopically. Three patients (11.1%) required open cystotomy and one underwent a transperineal urethrotomy. Four patients (14.8%) represented after discharge. Two patients had repeat presentations for genitourinary foreign body insertion. A significant monotonic positive trend of foreign body presentations was identified (Mann-Kendall tau = 0.95, P = 0.043). Presentations of genitourethral foreign body insertions have trended upward over the past two decades. However, most cases were successfully managed endoscopically.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"185-188"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142670084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High sperm DNA fragmentation - finding a needle in the haystack: tips on selecting the best sperm for ICSI and ART. 精子 DNA 碎片率高--大海捞针：为 ICSI 和 ART 挑选最佳精子的技巧。

Asian journal of andrology Pub Date : 2025-03-01 Epub Date: 2024-09-03 DOI: 10.4103/aja202451

Elisabetta Baldi, Giovanni M Colpi, Zhong-Wei Huang, Balasingam Balagobi, Florence Boitrelle, Rupin Shah, Ashok Agarwal

引用次数: 0

Exploring the clinical implications of novel SRD5A2 variants in 46,XY disorders of sex development. 探索新型 SRD5A2 变异对 46,XY 性发育障碍的临床影响。

Asian journal of andrology Pub Date : 2025-03-01 Epub Date: 2024-09-24 DOI: 10.4103/aja202469

Yu Mao, Jian-Mei Huang, Yu-Wei Chen-Zhang, He Lin, Yu-Huan Zhang, Ji-Yang Jiang, Xue-Mei Wu, Ling Liao, Yun-Man Tang, Ji-Yun Yang

{"title":"Exploring the clinical implications of novel SRD5A2 variants in 46,XY disorders of sex development.","authors":"Yu Mao, Jian-Mei Huang, Yu-Wei Chen-Zhang, He Lin, Yu-Huan Zhang, Ji-Yang Jiang, Xue-Mei Wu, Ling Liao, Yun-Man Tang, Ji-Yun Yang","doi":"10.4103/aja202469","DOIUrl":"10.4103/aja202469","url":null,"abstract":"Abstract: This study was conducted retrospectively on a cohort of 68 patients with steroid 5 α-reductase 2 (SRD5A2) deficiency and 46,XY disorders of sex development (DSD). Whole-exon sequencing revealed 28 variants of SRD5A2 , and further analysis identified seven novel mutants. The preponderance of variants was observed in exon 1 and exon 4, specifically within the nicotinamide adenine dinucleotide phosphate (NADPH)-binding region. Among the entire cohort, 53 patients underwent initial surgery at Sichuan Provincial People's Hospital (Chengdu, China). The external genitalia scores (EGS) of these participants varied from 2.0 to 11.0, with a mean of 6.8 (standard deviation [s.d.]: 2.5). Thirty patients consented to hormone testing. Their average testosterone-to-dihydrotestosterone (T/DHT) ratio was 49.3 (s.d.: 23.4). Genetic testing identified four patients with EGS scores between 6 and 9 as having this syndrome; and their T/DHT ratios were below the diagnostic threshold. Furthermore, assessments conducted using the crystal structure of human SRD5A2 have provided insights into the potential pathogenic mechanisms of these novel variants. These mechanisms include interference with NADPH binding (c.356G>C, c.365A>G, c.492C>G, and c.662T>G) and destabilization of the protein structure (c.727C>T). The c.446-1G>T and c.380delG variants were verified to result in large alterations in the transcripts. Seven novel variations were identified, and the variant database for the SRD5A2 gene was expanded. These findings contribute to the progress of diagnostic and therapeutic approaches for individuals with SRD5A2 deficiency.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"211-218"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142309351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Associations of Ureaplasma urealyticum infection with male infertility and intrauterine insemination outcomes. 尿解支原体感染与男性不育症和宫腔内人工授精结果的关系。

Asian journal of andrology Pub Date : 2025-03-01 Epub Date: 2024-11-08 DOI: 10.4103/aja202489

Yang-Yang Wan, Xiao-Yun Shi, Wen-Jing Liu, Shun Bai, Xin Chen, Si-Yao Li, Xiao-Hua Jiang, Li-Min Wu, Xian-Sheng Zhang, Juan Hua

{"title":"Associations of Ureaplasma urealyticum infection with male infertility and intrauterine insemination outcomes.","authors":"Yang-Yang Wan, Xiao-Yun Shi, Wen-Jing Liu, Shun Bai, Xin Chen, Si-Yao Li, Xiao-Hua Jiang, Li-Min Wu, Xian-Sheng Zhang, Juan Hua","doi":"10.4103/aja202489","DOIUrl":"10.4103/aja202489","url":null,"abstract":"Abstract: Ureaplasma urealyticum (UU) is one of the most commonly occurring pathogens associated with genital tract infections in infertile males, but the impact of seminal UU infection in semen on intrauterine insemination (IUI) outcomes is poorly understood. We collected data from 245 infertile couples who underwent IUI at The First Affiliated Hospital of USTC (Hefei, China) between January 2021 and January 2023. The subjects were classified into two groups according to their UU infection status: the UU-positive group and the UU-negative group. We compared semen parameters, pregnancy outcomes, and neonatal birth outcomes to investigate the impact of UU infection on IUI outcomes. There were no significantly statistical differences in various semen parameters, including semen volume, sperm concentration, total and progressive motility, sperm morphology, leukocyte count, the presence of anti-sperm antibody, and sperm DNA fragmentation index (DFI), between the UU-positive and UU-negative groups of male infertile patients (all P > 0.05). However, the high DNA stainability (HDS) status of sperm differed between the UU-positive and UU-negative groups, suggesting that seminal UU infection may affect sperm nuclear maturation ( P = 0.04). Additionally, there were no significant differences in pregnancy or neonatal birth outcomes between the two groups (all P > 0.05). These results suggest that IUI remains a viable and cost-effective option for infertile couples with UU infection who are facing infertility issues.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"219-224"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel biallelic MCMDC2 variants were associated with meiotic arrest and nonobstructive azoospermia. 新型双等位基因MCMDC2变异与减数分裂停止和非阻塞性无精子症有关。

Asian journal of andrology Pub Date : 2025-03-01 Epub Date: 2025-01-10 DOI: 10.4103/aja202495

Hao-Wei Bai, Na Li, Yu-Xiang Zhang, Jia-Qiang Luo, Ru-Hui Tian, Peng Li, Yu-Hua Huang, Fu-Rong Bai, Cun-Zhong Deng, Fu-Jun Zhao, Ren Mo, Ning Chi, Yu-Chuan Zhou, Zheng Li, Chen-Cheng Yao, Er-Lei Zhi

{"title":"Novel biallelic MCMDC2 variants were associated with meiotic arrest and nonobstructive azoospermia.","authors":"Hao-Wei Bai, Na Li, Yu-Xiang Zhang, Jia-Qiang Luo, Ru-Hui Tian, Peng Li, Yu-Hua Huang, Fu-Rong Bai, Cun-Zhong Deng, Fu-Jun Zhao, Ren Mo, Ning Chi, Yu-Chuan Zhou, Zheng Li, Chen-Cheng Yao, Er-Lei Zhi","doi":"10.4103/aja202495","DOIUrl":"10.4103/aja202495","url":null,"abstract":"Abstract: Nonobstructive azoospermia (NOA), one of the most severe types of male infertility, etiology often remains unclear in most cases. Therefore, this study aimed to detect four biallelic detrimental variants (0.5%) in the minichromosome maintenance domain containing 2 ( MCMDC2 ) genes in 768 NOA patients by whole-exome sequencing (WES). Hematoxylin and eosin (H&E) demonstrated that MCMDC2 deleterious variants caused meiotic arrest in three patients (c.1360G>T, c.1956G>T, and c.685C>T) and hypospermatogenesis in one patient (c.94G>T), as further confirmed through immunofluorescence (IF) staining. The single-cell RNA sequencing data indicated that MCMDC2 was substantially expressed during spermatogenesis. The variants were confirmed as deleterious and responsible for patient infertility through bioinformatics and in vitro experimental analyses. The results revealed four MCMDC2 variants related to NOA, which contributes to the current perception of the function of MCMDC2 in male fertility and presents new perspectives on the genetic etiology of NOA.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"268-275"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142960177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Androgen receptor inhibitors in treating prostate cancer. 治疗前列腺癌的雄激素受体抑制剂。

Asian journal of andrology Pub Date : 2025-03-01 Epub Date: 2024-11-19 DOI: 10.4103/aja202494

Ryan N Cole, Qinghua Fang, Kanako Matsuoka, Zhou Wang

{"title":"Androgen receptor inhibitors in treating prostate cancer.","authors":"Ryan N Cole, Qinghua Fang, Kanako Matsuoka, Zhou Wang","doi":"10.4103/aja202494","DOIUrl":"10.4103/aja202494","url":null,"abstract":"Abstract: Androgens play an important role in prostate cancer development and progression. Androgen action is mediated through the androgen receptor (AR), a ligand-dependent DNA-binding transcription factor. AR is arguably the most important target for prostate cancer treatment. Current USA Food and Drug Administration (FDA)-approved AR inhibitors target the ligand-binding domain (LBD) and have exhibited efficacy in prostate cancer patients, particularly when used in combination with androgen deprivation therapy. Unfortunately, patients treated with the currently approved AR-targeting agents develop resistance and relapse with castration-resistant prostate cancer (CRPC). The major mechanism leading to CRPC involves reactivation of AR signaling mainly through AR gene amplification, mutation, and/or splice variants. To effectively inhibit the reactivated AR signaling, new approaches to target AR are being actively explored. These new approaches include novel small molecule inhibitors targeting various domains of AR and agents that can degrade AR. The present review provides a summary of the existing FDA-approved AR antagonists and the current development of some of the AR targeting agents.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"144-155"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142670082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between maximal urethral length preservation and postoperative continence after robot-assisted radical prostatectomy: a meta-analysis and systematic review. 机器人辅助根治性前列腺切除术后最大尿道长度保留与术后尿失禁之间的关系：一项荟萃分析和系统综述。

Asian journal of andrology Pub Date : 2025-03-01 Epub Date: 2024-10-22 DOI: 10.4103/aja202481

Tian-Yu Xiong, Zhan-Liang Liu, Hao-Yu Wu, Yun-Peng Fan, Yi-Nong Niu

{"title":"Association between maximal urethral length preservation and postoperative continence after robot-assisted radical prostatectomy: a meta-analysis and systematic review.","authors":"Tian-Yu Xiong, Zhan-Liang Liu, Hao-Yu Wu, Yun-Peng Fan, Yi-Nong Niu","doi":"10.4103/aja202481","DOIUrl":"10.4103/aja202481","url":null,"abstract":"Abstract: Urinary incontinence is a common complication following robot-assisted radical prostatectomy (RARP). Urethral length has been identified as a factor affecting postoperative continence recovery. In this meta-analysis, we examined the association between use of the maximal urethral length preservation (MULP) technique and postoperative urinary continence in patients undergoing RARP. We conducted a comprehensive search of PubMed, Web of Science, Embase, and the Cochrane Library up to December 31, 2023. The quality of the literature was assessed using the Newcastle-Ottawa Scale. A random-effects meta-analysis was performed to synthesize data and calculate the odds ratio (OR) from eligible studies on continence and MULP. Six studies involving 1869 patients met the eligibility criteria. MULP was positively associated with both early continence (1 month after RARP; Z = 3.62, P = 0.003, OR = 3.10, 95% confidence interval [CI]: 1.68-5.73) and late continence (12 months after RARP; Z = 2.34, P = 0.019, OR = 2.10, 95% CI: 1.13-3.90). Oncological outcomes indicated that MULP did not increase the overall positive surgical margin rate or the positive surgical margin status at the prostate apex (both P > 0.05). In conclusion, the use of the MULP technique in RARP significantly improved both early and late postoperative continence outcomes without compromising oncological outcomes.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"225-230"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the influence of the sY1192 gene locus in the AZFb/c region on sperm quality and pregnancy outcome. 研究 AZFb/c 地区 sY1192 基因位点对精子质量和妊娠结果的影响。

Asian journal of andrology Pub Date : 2025-03-01 Epub Date: 2024-10-18 DOI: 10.4103/aja202478

Gang-Xin Chen, Yan Sun, Rui Yang, Zhi-Qing Huang, Hai-Yan Li, Bei-Hong Zheng

{"title":"Study on the influence of the sY1192 gene locus in the AZFb/c region on sperm quality and pregnancy outcome.","authors":"Gang-Xin Chen, Yan Sun, Rui Yang, Zhi-Qing Huang, Hai-Yan Li, Bei-Hong Zheng","doi":"10.4103/aja202478","DOIUrl":"10.4103/aja202478","url":null,"abstract":"Abstract: Y chromosome microdeletions are an important cause of male infertility. At present, research on the Y chromosome is mainly focused on analyzing the loss of large segments of the azoospermia factor a/b/c (AZFa/b/c) gene, and few studies have reported the impact of unit point deletion in the AZF band on fertility. This study analyzed the effect of sperm quality after sY1192 loss in 116 patients. The sY1192-independent deletion accounted for 41.4% (48/116). Eight patterns were found in the deletions associated with sY1192. The rate of sperm detection was similar in the semen of patients with the independent sY1192 deletion and the combined sY1192 deletions (52.1% vs 50.0%). The patients with only sY1192 gene loss had a higher probability of sperm detection than the patients whose sY1192 gene locus existed, but other gene loci were lost (52.1% vs 32.0%). The hormone levels were similar in patients with sY1192 deletion alone and in those with sY1192 deletion and other types of microdeletions in the presence of the sY1192 locus. After multiple intracytoplasmic sperm injection (ICSI) attempts, the pregnancy rate of spouses of men with sY1192-independent deletions was similar to that of other types of microdeletions, but the fertilization and cleavage rates were higher. We observed that eight deletion patterns were observed for sY1192 microdeletions of AZFb/c, dominated by the independent deletion of sY1192. After ICSI, the fertilization rate and cleavage rate of the sY1192-independent microdeletion were higher than those of other Y chromosome microdeletion types, but there was no significant difference in pregnancy outcomes.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"231-238"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of IGF2 levels with sperm quality, inflammation, and DNA damage in infertile patients. 不育患者体内 IGF2 水平与精子质量、炎症和 DNA 损伤的相关性。

Asian journal of andrology Pub Date : 2025-03-01 Epub Date: 2024-10-29 DOI: 10.4103/aja202487

Jing-Gen Wu, Cai-Ping Zhou, Wei-Wei Gui, Zhong-Yan Liang, Feng-Bin Zhang, Ying-Ge Fu, Rui Li, Fang Wu, Xi-Hua Lin

{"title":"Correlation of IGF2 levels with sperm quality, inflammation, and DNA damage in infertile patients.","authors":"Jing-Gen Wu, Cai-Ping Zhou, Wei-Wei Gui, Zhong-Yan Liang, Feng-Bin Zhang, Ying-Ge Fu, Rui Li, Fang Wu, Xi-Hua Lin","doi":"10.4103/aja202487","DOIUrl":"10.4103/aja202487","url":null,"abstract":"Abstract: Insulin-like growth factor 2 (IGF2) is a critical endocrine mediator implicated in male reproductive physiology. To investigate the correlation between IGF2 protein levels and various aspects of male infertility, specifically focusing on sperm quality, inflammation, and DNA damage, a cohort of 320 male participants was recruited from the Women's Hospital, Zhejiang University School of Medicine (Hangzhou, China) between 1 st January 2024 and 1 st March 2024. The relationship between IGF2 protein concentrations and sperm parameters was assessed, and Spearman correlation and linear regression analysis were employed to evaluate the independent associations between IGF2 protein levels and risk factors for infertility. Enzyme-linked immunosorbent assay (ELISA) was used to measure IGF2 protein levels in seminal plasma, alongside markers of inflammation (tumor necrosis factor-alpha [TNF-α] and interleukin-1β [IL-1β]). The relationship between seminal plasma IGF2 protein levels and DNA damage marker phosphorylated histone H2AX (γ-H2AX) was also explored. Our findings reveal that IGF2 protein expression decreased notably in patients with asthenospermia and teratospermia. Correlation analysis revealed nuanced associations between IGF2 protein levels and specific sperm parameters, and low IGF2 protein concentrations correlated with increased inflammation and DNA damage in sperm. The observed correlations between IGF2 protein levels and specific sperm parameters, along with its connection to inflammation and DNA damage, underscore the importance of IGF2 in the broader context of male reproductive health. These findings lay the groundwork for future research and potential therapeutic interventions targeting IGF2-related pathways to enhance male fertility.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"204-210"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142523808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A convenient research strategy for functional verification of epigenetic regulators during spermatogenesis. 精子发生过程中表观遗传调节因子功能验证的便捷研究策略。

Asian journal of andrology Pub Date : 2025-03-01 Epub Date: 2024-09-17 DOI: 10.4103/aja202453

Shan Li, Ying Yuan, Ke-Yu Zhang, Yi-Dan Guo, Lu-Tong Wang, Xiao-Yuan Zhang, Shu Zhang, Qi Yan, Rong Zhang, Jie Chen, Feng-Tang Yang, Jing-Rui Li

{"title":"A convenient research strategy for functional verification of epigenetic regulators during spermatogenesis.","authors":"Shan Li, Ying Yuan, Ke-Yu Zhang, Yi-Dan Guo, Lu-Tong Wang, Xiao-Yuan Zhang, Shu Zhang, Qi Yan, Rong Zhang, Jie Chen, Feng-Tang Yang, Jing-Rui Li","doi":"10.4103/aja202453","DOIUrl":"10.4103/aja202453","url":null,"abstract":"Abstract: Spermatogenesis is a fundamental process that requires a tightly controlled epigenetic event in spermatogonial stem cells (SSCs). The mechanisms underlying the transition from SSCs to sperm are largely unknown. Most studies utilize gene knockout mice to explain the mechanisms. However, the production of genetically engineered mice is costly and time-consuming. In this study, we presented a convenient research strategy using an RNA interference (RNAi) and testicular transplantation approach. Histone H3 lysine 9 (H3K9) methylation was dynamically regulated during spermatogenesis. As Jumonji domain-containing protein 1A (JMJD1A) and Jumonji domain-containing protein 2C (JMJD2C) demethylases catalyze histone H3 lysine 9 dimethylation (H3K9me2), we firstly analyzed the expression profile of the two demethylases and then investigated their function. Using the convenient research strategy, we showed that normal spermatogenesis is disrupted due to the downregulated expression of both demethylases. These results suggest that this strategy might be a simple and alternative approach for analyzing spermatogenesis relative to the gene knockout mice strategy.","PeriodicalId":93889,"journal":{"name":"Asian journal of andrology","volume":" ","pages":"261-267"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142303267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0