Acta odontologica latinoamericana : AOL最新文献

{"title":"Trigeminal neuroplasticity after pulpitis and oral administration ofparacetamol in rats.","authors":"Mariela Celeste Canzobre, Yamila Boix, Maria E Miyashiro, Alejandra R Paganelli, Laura R Caltana, Hugo Ríos","doi":"10.54589/aol.39/1/83","DOIUrl":"10.54589/aol.39/1/83","url":null,"abstract":"<p><p>Experimental stimulation of dental pulp nociceptors induces plastic changes in the trigeminal subnucleus caudalis (Vc) through increased intracellular calcium levels and discharge of nociceptive afferent fibers. Acetaminophen (paracetamol) is a potent and selective inhibitor of COX-2 within the central nervous system (CNS) and may act in association with the cannabinoid system and the descending serotonergic pathway.</p><p><strong>Aim: </strong>In this context, the main objective of this study is to analyze the plastic changes induced by paracetamol in the nociceptive circuit of the Vc following unilateral dental pulp inflammation (pulpitis) in rats.</p><p><strong>Material and method: </strong>Twenty-one female Wistar rats, 50 days old, were divided into three groups. In the experimental group 1 (EG1), a unilateral pulpitis was induced in the left mandibular first molar, and animals received no further treatment. In group 2 (EG2), the procedure was the same as in EG1, but 48 h later, animals received paracetamol in 3 daily doses (300 mg/kg each) every 4 h for 2 days. The control group (CG) received no treatment. After euthanasia, liver, hemimandibles and brainstem samples were analyzed. The results were analyzed using Student's t-test or ANOVA (p<0.05).</p><p><strong>Results: </strong>5-hydroxytryptamine transporter (5HT-T) analysis in the ipsilateral Vc demonstrated that paracetamol administration increased plexus length and varicosity density compared with EG1 and CG (p<0.05). Paracetamol also increased cannabinoid CB1 receptor (CB1r) expression (p<0.05), and decreased the number of calbindin D28K positive projection neurons (p<0.05).</p><p><strong>Conclusion: </strong>Following experimental pulpitis, oral administration of paracetamol induces plastic changes in the serotonergic and endocannabinoid systems within the Vc. This plasticity is reflected by a reduced number of fusiform calbindin D28K projection neurons in lamina I, associated with modulation of orofacial nociceptive processing.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"39 1","pages":"83-92"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Validation of an Instrument to Assess Awake Bruxism in Adults.","authors":"Maria Andrada, Miguel Wilken, Federico Stolbizer, Pablo Salgado, Malco Rossi, Marcelo Merello","doi":"10.54589/aol.39/1/10","DOIUrl":"10.54589/aol.39/1/10","url":null,"abstract":"<p><p>The definition of bruxism has evolved over the past few years. Currently, two clinical presentations are recognized: Sleep Bruxism (SB) and Awake Bruxism (AB). Although various bruxism assessment strategies have been developed to guide clinical practice, no specific, valid, reliable tool has been designed specifically for patients with AB.</p><p><strong>Aim: </strong>To design and validate a questionnaire to assess the presence, severity and symptomatology of AB. Secondary objectives included exploring the clinical and psychiatric manifestations of AB.</p><p><strong>Materials and method: </strong>A prospective case-control study was conducted on 14 AB cases and 20 healthy controls. The presence or absence of the condition was confirmed by electromyographic study. A specific questionnaire for AB (AB-Q) was developed, and AB psychometric properties were evaluated. The AB-Q consists of 24 items grouped into four domains. The reliability, sensitivity and specificity of the AB-Q were assessed.</p><p><strong>Results: </strong>Significant differences were observed between groups: EMG-confirmed bruxism episodes were recorded in all 14 AB patients and none of the controls. The AB-Q showed high internal consistency (Cronbach's alpha: 0.924) and strong discriminative capacity, with a sensitivity of 100% and a specificity of 90%. A significant association was found between bruxism and the presence of anxiety and depressive disorders, as well as obsessivecompulsive traits.</p><p><strong>Conclusion: </strong>The AB-Q questionnaire proved to be a useful tool with acceptable clinimetric properties for assessing the presence and severity of bruxism.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"39 1","pages":"10-20"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An ex vivo comparison of bond strength measured by the push-out test between AH Plus and Endosequence BC sealer Hiflow.","authors":"Thayane da Costa Pinheiro, Carlos E da Silveira Bueno, Alexandre Sigrist De Martin, Carolina Pessoa Stringheta, Carlos E Fontana, Rina A Pelegrine, Ana G Limoeiro, Wayne M Nascimento, Marilia Fagury Videira Marceliano-Alves, Thais Machado de Carvalho Coutinho, Cesar A Perini Rosas, Daniel G Pedro Rocha","doi":"10.54589/aol.38/3/210","DOIUrl":"10.54589/aol.38/3/210","url":null,"abstract":"<p><strong>Introduction: </strong>Endodontic obturation is fundamental to treatment success, with the bond strength of sealer cements to dentin being a crucial parameter for evaluating their effectiveness.</p><p><strong>Aim: </strong>To evaluate the bond strength between root canal dentin walls and the obturation materials AH Plus and Endosequence BC Sealer HiFlow using the push-out test.</p><p><strong>Material and method: </strong>Twenty single-rooted mandibular premolars were divided into two groups (n=10) according to the sealer used: Group AH (AH Plus Sealer) and Group HF (Endosequence BC Sealer HiFlow). The canals were instrumented with Wave One Gold Large, using 20 mL of 2.5% sodium hypochlorite as irrigation solution. The teeth were cut into 1 mm thick slices in the cervical and middle third regions to perform the push-out test. The failure mode was observed under a stereomicroscope at 40x magnification. The results were subjected to Tukey's test and ANOVA with a 5% significance level.</p><p><strong>Results: </strong>In the push-out bond strength test, there was no statistically significant difference between AH Plus and Endosequence BC Sealer HiFlow cements (p=0.1952). Similarly, the failure modes showed no significant difference between the cements, in both the cervical and middle thirds (p=0.5703).</p><p><strong>Conclusion: </strong>AH Plus and Endosequence BC Sealer HiFlow demonstrated similar behavior in terms of bond strength and failure mode in root canal dentin walls.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"38 3","pages":"210-215"},"PeriodicalIF":0.0,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12937117/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147292083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of compressive and flexural properties of three contemporary bulk fill resin composites.","authors":"Norma Borja-Farfán, Danilo Loyola-Livias, Leslie Casas-Apayco, Monica Hermoza-Novoa","doi":"10.54589/aol.38/3/237","DOIUrl":"10.54589/aol.38/3/237","url":null,"abstract":"<p><strong>Introduction: </strong>Contemporary restorative materials must demonstrate how much stress they can withstand before permanently deforming under the constant forces generated during chewing.</p><p><strong>Aim: </strong>To compare the compressive and flexural strength of three bulk fill resin composites.</p><p><strong>Materials and method: </strong>This study analyzed three bulk fill resin composites: Opus™ bulk fill (OBF), Tetric® N-Ceram bulk fill (TNC), and Filtek™ bulk fill (FBF), and one conventional resin composite: Filtek™ Z350XT (FZXT) as a control group. Each composite was used to prepare 14 cylindrical specimens (n=56) for compressive strength tests, and 19 rectangular specimens (n=76) for flexural strength tests. Mechanical tests were performed using an Instron® universal testing machine - 100 kN load at a speed of 1 mm/1min.</p><p><strong>Results: </strong>Descriptive (Mean ± SD) and inferential statistics (Kruskal-Wallis test/ post-hoc test), p<0.05 were used. The compressive test results (Means ± SD) were: OBF (141.68MPa ± 22.20), TNC (139.03MPa ± 23.56), FBF (235.59MPa ± 26.08), and FZXT (99.28MPa ± 11.36). The flexural test results (Means ± SD) were: OBF (116.29 ± 7.20), TNCBF (109.67MPa ± 7.58), FBF (200.53MPa ± 10.32), and FZXT (90.08MPa ± 8.63). Statistically significant differences were found between the resin composites (p<0.001) in both mechanical tests.</p><p><strong>Conclusions: </strong>The bulk fill resin composites demonstrated higher compressive and flexural strength than the conventional resin composite. However, the use of these restorative materials may depend on specific clinical requirements and material handling preferences.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"38 3","pages":"237-241"},"PeriodicalIF":0.0,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12937118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147292147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluoride content in children's dentifrices marketed in Argentina.","authors":"Ana Sorazabal, Lidia Ar Valadas, Angela B Argentieri, Pablo Salgado, Thereza C Farias Botelho Dantas, Celiane M Carneiro Tapety, Aldo Squassi","doi":"10.54589/aol.38/3/242","DOIUrl":"10.54589/aol.38/3/242","url":null,"abstract":"<p><strong>Introduction: </strong>Fluoridated dentifrices are the most widely used formulations for caries prevention and control. However, there is great concern regarding legislation on the use of fluoride formulations in early childhood from the standpoint of avoiding increased risk of fluorosis while at the same time achieving a concentration that ensures anticaries activity.</p><p><strong>Aim: </strong>The aim of this study was to evaluate the stability of fluoride concentration in the main children's toothpaste brands marketed in Argentina.</p><p><strong>Materials and method: </strong>Twenty different brands of children's toothpaste were evaluated by analyzing the concentration of total fluoride (TF), total soluble fluoride (TSF) and ionic fluoride (IF) in two or three samples of each brand. Each sample was analyzed in duplicate using an ion-specific electrode calibrated with fluoride standards, and the results were expressed in ppm (µg F/g).</p><p><strong>Results: </strong>TF was found to be lower than stated by the manufacturer in 85% of the brands analyzed. TF concentration found in fluoride toothpastes ranged from 651.5 to 1090.4 μg/ml F-, while TSF ranged from 504.2 to 915.5 μg/ml F, and IF from 298.6 to 838.2 μg/ml F-.</p><p><strong>Conclusions: </strong>The formulation and fluoride content in children's toothpastes marketed in Argentina vary greatly according to brand. In all the samples, TF concentration was lower than 1500 ppm F-, and TSF concentration was lower than 1000 ppm F-, the minimum amount necessary to ensure anticaries effect. This indicates the need for improved formulations and regulations.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"38 3","pages":"242-250"},"PeriodicalIF":0.0,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12937122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147292139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between dental caries and sociodemographic factors in children aged 5-12 years.","authors":"Elmer Jehovani Portillo-Sorto","doi":"10.54589/aol.38/3/183","DOIUrl":"10.54589/aol.38/3/183","url":null,"abstract":"<p><strong>Introduction: </strong>Dental caries is a global public health issue, particularly in children, and is influenced by sociodemographic conditions.</p><p><strong>Aim: </strong>To assess the association between dental caries and sociodemographic factors in children aged 5-12 years in Victoria, Cabañas, El Salvador.</p><p><strong>Materials and methods: </strong>A cross-sectional, correlational study was conducted among 137 children (48.2% boys, 51.8%girls), measuring dental caries using DMFT and dmft indices, and oral hygiene using the O'LearyPlaque Index. Sociodemographic data were obtained through caregiver questionnaires.</p><p><strong>Results: </strong>Caries prevalence was 63.5%. DMFT increased with age (highest in 10-12-year-old girls: mean = 5.44), while dmft declined (lowest in 12-year-old girls: mean = 0.56). Most children (98.5%) had poor oral hygiene. A weak but significant negative correlation was found between income and dmft (ρ = -0.189; p = 0.027). No other significant association was observed between caries indices and maternal education, income, or plaque index.</p><p><strong>Conclusions: </strong>Caries patterns differed significantly according to age and sex. Despite the high prevalence, most sociodemographic variables showed no strong statistical association with caries. Preventive interventions should focus on age- and sex-specific risks.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"38 3","pages":"183-194"},"PeriodicalIF":0.0,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12937121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147292078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of root canal mechanical preparation with two systems with different kinematics. Ex vivo micro-CT study.","authors":"Li Ting Lee, María E Santillán, Eugenia Miklaszewski, María L Cabirta, Liliana G Sierra, Pablo A Rodríguez","doi":"10.54589/aol.38/3/202","DOIUrl":"10.54589/aol.38/3/202","url":null,"abstract":"<p><strong>Introduction: </strong>Oval and ribbed root canals present a major challenge in endodontic treatment due to their complex anatomy, which fosters the accumulation of debris and bacteria. Mechanical preparation systems have been developed to improve shaping efficiency and canal centricity in these anatomically complex canals.</p><p><strong>Aim: </strong>To compare, using micro-computed tomography, pulp space volume increase, untouched canal surface area, and apical transportation in oval root canals prepared with WaveOne Gold (WOG) or XP-endo Shaper (XPS).</p><p><strong>Materials and method: </strong>30 extracted single-rooted upper second premolars with oval root canals were randomly divided into two experimental groups and instrumented with WOG (n: 15) or XPS (n: 15). All specimens were scanned with micro-CT (SkyScan 1272; Bruker-microCT) for comparison before and after instrumentation using the following acquisition parameters: 19µm voxel, 30 kV, 800 mA, 0.6° rotation step, 360° rotation and 1 mm thick aluminum filter. Initial and final volumes, surface areas and centricities were analyzed to calculate pulp space volume increase, untouched surface area and transportation. Data were analyzed using paired Student's t-tests, Wilcoxon signed ranks or Mann-Whitney U tests, as appropriate (p < 0.05 significant).</p><p><strong>Results: </strong>There was no significant difference between WOG and XPS in pulp space volume increase (p=0.26) or untouched surface area (p>0.99). However, there were significant differences in centricity on the X and Y axes (p<0.05), while on the Z axis, the differences were not significant (p=0.05).</p><p><strong>Conclusion: </strong>WOG and XPS had similar shaping effectiveness. All files were able to clean and shape moderately curved canals with minimal apical transportation.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"38 3","pages":"202-209"},"PeriodicalIF":0.0,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12937120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147292097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Midpalatal suture expansion: an in vivo histological and immunohistochemical study of the impact of force magnitude.","authors":"Mónica I Yamauchi, Rubén Piña Lugo, Luciana M Sánchez, Romina C De Lucca, Carola B Bozal","doi":"10.54589/aol.38/3/225","DOIUrl":"10.54589/aol.38/3/225","url":null,"abstract":"<p><strong>Introduction: </strong>Although there is sufficient clinical evidence regarding the effectiveness of rapid expansion of the middle palate, some of the biological responses to variables such as the magnitude of the expansive force are still unknown.</p><p><strong>Aim: </strong>The aim of this study was to conduct a qualitative and quantitative analysis of the response of midpalatal suture tissues in growing rats exposed to expansive forces of different magnitudes.</p><p><strong>Materials and method: </strong>Twenty-four 7-week-old male Wistar rats were divided into 3 groups: a control group (C) and two experimental groups to which 60 g or 90 g expansive forces were applied for 7 days (E60 and E90, respectively). Frontal sections of the upper maxillae were stained with haematoxylin-eosin, Masson's trichrome and PAS-Alcian Blue. The expression of runt related transcription factor 2 (Runx2) and receptor activator of nuclear factor kappa B ligand (RANKL) was evaluated by immunohistochemical staining.</p><p><strong>Results: </strong>The degree of expansion and the biological response of the cells involved in the suture ossification process were evaluated histomorphometrically. ANOVA was used for statistical comparisons. Both the expansive forces applied caused significant increases in maxillary width and suture area. Group E60 showed qualitative changes in the composition of the cartilage extracellular matrix, a significant increase in the percentage of Runx2+ mesenchymal like cells, and a significant reduction in the number of RANKL+ chondrocytes/mm2.</p><p><strong>Conclusions: </strong>These results show that lower expansive forces would stimulate osteogenesis in a direct manner not associated to the endochondral ossification of suture secondary cartilage, enabling partial elucidation of how bone resorption and formation are regulated during rapid maxillary expansion.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"38 3","pages":"225-236"},"PeriodicalIF":0.0,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12937119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147291064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective analysis of guided access technique in calcified canals using cone beam tomography and digital scanning.","authors":"Bruna de Athayde Casadei, Alexandre Sigrist De Martin, Rina A Pelegrine, Wayne Martins Nascimento, Daniel Guimarães Pedro Rocha, Carlos E Fontana, Vinicius de Carvalho Machado, Carolina Pessoa Stringheta, Ana G Limoeiro, Michel Klymus, Thais Machado de Carvalho Coutinho, Marilia Fagury Videira Marceliano-Alves, Karoline de Oliveira Vieira Clemente, Carlos E da Silveira Bueno","doi":"10.54589/aol.38/3/195","DOIUrl":"10.54589/aol.38/3/195","url":null,"abstract":"<p><strong>Introduction: </strong>The endodontic treatment of teeth presenting with calcified pulp is associated with significant technical difficulties.</p><p><strong>Aim: </strong>To evaluate the success rate of the guided access technique for root canals using cone beam computed tomography (CBCT) between 2019 and 2021.</p><p><strong>Material and method: </strong>The study selected cases of anterior and posterior teeth with severe pulp calcification, totaling 100 teeth including 59 incisors, 2 canines, 8 premolars, and 31 molars. CBCT scans were performed for all patients, and a virtual burr path was planned on a computer screen to guide the drill to the beginning of the root canal. The virtual drill path was delineated based on axial and cross-sectional tomographic data, and a template was fabricated using Computer Numerical Control (CNC) technology. Success was determined by reaching the canal lumen without deviation from the original path.</p><p><strong>Results: </strong>The success rate of the guided access technique was 99.1%, with calcification diagnosed in 48.7% of cases. Clinical history revealed deep restoration and necrosis in 34.8% of cases, and trauma in 34.8%. In 65.2% of cases, an attempt was made before referral for guided access.</p><p><strong>Conclusion: </strong>The guided access technique demonstrated high success rates, particularly when appropriately indicated. Clinical relevance: Guided access technique provides a reliable option for managing calcified root canals, thereby improving treatment outcomes.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"38 3","pages":"195-201"},"PeriodicalIF":0.0,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12937121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147291166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological evaluation of dentin-pulp tissue response after transplantation of stem cells from periosteum and human deciduous teeth and in rats.","authors":"Tainara B Yamanaka, Randro M Rajab, Rina A Pelegrine, Carlos E da Silveira Bueno, Alexandre Sigrist De Martin, Carolina Pessoa Stringheta, Elizabeth Ferreira Martinez, Carlos E Fontana, Sérgio L Pinheiro, Daniel Guimarães Pedro Rocha, Larissa Regina Kuntze Dos Santos, André A Pelegrine","doi":"10.54589/aol.38/3/216","DOIUrl":"10.54589/aol.38/3/216","url":null,"abstract":"<p><strong>Introduction: </strong>Endodontic treatment offers a high success rate; however, the search for therapeutic alternatives that promote regeneration of the dentin-pulp complex has elicited great interest.</p><p><strong>Aim: </strong>The aim of this study was to evaluate, in an in vivo model, the potential for dentin-pulp regeneration by transplantation of stem cells from two sources: pulp of human deciduous teeth and periosteum.</p><p><strong>Materials and method: </strong>Eighteen mesiobuccal canals of maxillary first molars of immunosuppressed rats were distributed into three groups (n=6): group DT (deciduous teeth pulp), received stem cells from deciduous tooth pulp in hydrogel vehicle; group P (periosteal), received periosteal stem cells in hydrogel vehicle; and group NC (negative control) received only hydrogel. After pulpectomy and canal preparation, the teeth received the respective materials, and the cavities were sealed. After 12 weeks, the animals were euthanized and the specimens underwent histological processing and qualitative evaluation of intracanal fibrous connective tissue, odontoblast-like cells, intracanal mineralized tissue, and inflammatory cell infiltrate.</p><p><strong>Results: </strong>In the NC group, abundant presence of inflammatory cells was observed throughout the canal, in addition to dentin chips and remnants of amorphous substance from pulp tissue. In groups DT and P, in the apical region, new tissue formation suggestive of repair was observed, with deposition of bone-like mineralized tissue and periodontal ligament-like connective tissue containing blood vessels and osteocyte- and fibroblast-like cells.</p><p><strong>Conclusions: </strong>Transplanted stem cells from pulp of human deciduous teeth and periosteum did not show potential for regeneration, but for tissue repair, with formation of periodontal ligament-like and bone-like tissue.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"38 3","pages":"216-224"},"PeriodicalIF":0.0,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12937123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147292067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}