Acta odontologica latinoamericana : AOL最新文献

{"title":"Radiographic study of mandibular alterations resulting from aketogenic diet in growing rats.","authors":"Silvia C Romano, Adrián E Martin, Gustavo M Rodríguez, María L Córdoba, María M Mir, María K Salum, Juan A Garat","doi":"10.54589/aol.39/1/30","DOIUrl":"10.54589/aol.39/1/30","url":null,"abstract":"<p><strong>Aim: </strong>This study analyzes the development of mandibular condyles and its likely impact on the direction of mandibular displacement during maxillofacial development in a ketogenic diet model in growing rats.</p><p><strong>Materials and method: </strong>Twenty male Wistar rats, weaned at 21 days, were assigned to one of the following groups: control (fed a regular hard diet ad libitum) and experimental (fed a ketogenic hard diet, ad libitum). After 30 days of diet, ketonemia was determined and animals were euthanized. The mandibles were resected, hemisected at the symphysis and fixed in 10% formalin. Remaining soft tissues were removed. Metallic landmarks were placed in the mandibular foramens of one hemimandible of each rat. The hemimandibles were radiographed and cephalometric study was performed on paper tracings of the projected image of the radiographs. Data obtained were analyzed with Student's \"t\" Test.</p><p><strong>Results: </strong>Food consumed was lower in the experimental group. Body weight was 179±1 g in controls, and 128±11 g in the experimental group, p<0.05. Ketonemia was 0.29±0.042mmol/l in controls and 1.89±0.7 mmol/l in the experimental group, p<0.05. Condylar process length and angular process convexity were significantly lower in the experimental group.</p><p><strong>Conclusions: </strong>These results suggest that the ketogenic diet alters the development of the condylar process and the angle of the mandible in rats, promoting a dolichofacial growth pattern.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"39 1","pages":"30-5"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric analysis of dental research in Peruvian universities, 2013-2023.","authors":"Cesar D Rojas-Senador, Neila Cristhine Quiroz Silva, María Claudia Garcés-Elías, Ana Isabel Correa-Orrego, Andrés A Agudelo-Suárez, Roberto A León-Manco","doi":"10.54589/aol.39/1/21","DOIUrl":"10.54589/aol.39/1/21","url":null,"abstract":"<p><p>Scientific production in dentistry has grown in Peru over the past decade; however, institutional differences in publication impact, thematic areas, and collaboration patterns remain unclear.</p><p><strong>Aim: </strong>To compare the Scopus-indexed dental scientific output of five Peruvian universities between 2013 and 2023.</p><p><strong>Materials and method: </strong>A cross-sectional bibliometric study was conducted using Scopus to identify dental publications affiliated with the five most productive Peruvian universities from 2013 to 2023. Eligibility criteria included dental-related topics and full variable availability. After screening 825 records, 584 were included. Extracted variables included publication year, type, thematic area, citations, scientific productivity, number of authors, and collaboration patterns. Descriptive indicators were computed; normality was assessed using Shapiro-Wilk. Group differences were tested with Chisquare and Kruskal-Wallis tests, with Bonferroni-adjusted post hoc analyses. Statistical significance was set at p<0.05.</p><p><strong>Results: </strong>The greatest increase in scientific output occurred between 2019 and 2023, peaking in 2021. Universidad Científica del Sur produced the highest number of publications (34.25%), while Universidad Peruana Cayetano Heredia obtained the highest citation impact. Public Oral Health was the most frequently represented area. Significant differences were found in thematic specialization (p=0.016), citation averages (p<0.001), number of authors (p=0.007), and collaboration types (p<0.001). National collaborations predominated, while international partnerships remained limited.</p><p><strong>Conclusions: </strong>Peruvian dental research indexed in Scopus has increased substantially in recent years. Citation impact and thematic specialization differ among universities, reflecting heterogeneous research development. The predominance of national collaboration highlights the need to reinforce international cooperative networks. These findings support the formulation of differentiated strategies to strengthen institutional research capacity and global visibility.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"39 1","pages":"21-29"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Penetration effect of sodium hypochlorite activated by the NSKVarios 370 ultrasonic system and the EQ-S sonic system in singlerooted premolars.","authors":"Manuel Alfredo Urrea Campoy, Julio César Garcia Briones, Eduardo Alfonso Campoy Félix, Leticia Yael Terán Espinoza, Javier Dominguez Bernal","doi":"10.54589/aol.39/1/101","DOIUrl":"10.54589/aol.39/1/101","url":null,"abstract":"<p><p>The effectiveness of endodontic disinfection largely depends on the ability of sodium hypochlorite (NaOCl) to penetrate deeply into dentinal tubules, particularly in the apical third, where the anatomy is more complex and mechanical access is limited. Sonic and ultrasonic activation techniques have been proposed to enhance this penetration by generating dynamic fluid movement.</p><p><strong>Aim: </strong>To evaluate and compare the depth of penetration of 5.25% NaOCl into dentinal tubules using passive ultrasonic irrigation (PUI; NSK Varios 370) and sonic activation (SIM; EQ-S Metabiomed), in comparison to conventional needle irrigation.</p><p><strong>Materials and method: </strong>thirty-six extracted human single-rooted premolars were instrumented to working length with ProTaper Gold F3. The teeth were randomly assigned to three groups (n = 12): PUI, SIM, and control. A standardized final irrigation protocol was performed, followed by the application of 1% methylene blue, activated or not, according to the group. Three transverse sections were obtained from each tooth (coronal, middle, and apical), and penetration depth was measured using ImageJ image analysis. Data were analyzed with nonparametric tests (Mann-Whitney U; p < 0.05).</p><p><strong>Results: </strong>Both PUI and SIM showed significantly greater penetration depths than the control group in all three thirds of the canal (p < 0.05). No significant difference was found between PUI and SIM (p > 0.05). The greatest penetration was observed in the coronal third, followed by the middle and apical thirds in all groups.</p><p><strong>Conclusions: </strong>Ultrasonic and sonic activation techniques produced greater marker penetration into dentinal tubules compared to conventional irrigation. Furthermore, the EQ-S system yielded similar results to those obtained with ultrasonic activation, with no statistically significant difference between the two systems.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"39 1","pages":"101-108"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of resveratrol-enriched endodontic sealants on pulp stem cell viability and collagen production.","authors":"Pedro Antunes Teixeira, Cláudia Fernandes de Magalhães Silveira, Alexandre Sigrist de Martin, Carlos Eduardo Silveira Bueno, João Pedro Rangel-Coelho, Elizabeth Ferreira Martinez","doi":"10.54589/aol.39/1/36","DOIUrl":"10.54589/aol.39/1/36","url":null,"abstract":"<p><p>Root canal sealers should provide effective sealing while remaining biocompatible with periapical tissues. Strategies to further improve their biological performance are desirable. Resveratrol, a bioactive polyphenol with antioxidant and regenerative properties, may enhance cellular responses and tissue repair, representing a promising adjunct for endodontic materials.</p><p><strong>Aim: </strong>The aim of this study was to evaluate cell viability and type I collagen expression in undifferentiated mesenchymal stem cell cultures exposed to EndoSequence BC bioceramic or AH Plus resin-based endodontic cements, with or without the addition of resveratrol.</p><p><strong>Materials and method: </strong>Cement specimens were prepared in standardized molds (5 mm × 2 mm), allowed to set for 48 hours, sterilized, and conditioned in culture medium at a concentration of 0.2 g/mL at 37 °C. Resveratrol was added to the culture media at concentrations of 0.25 μM and 0.5 μM. The mesenchymal cell cultures were exposed to the different experimental conditions for 24, 48 and 72 hours, after which viability was assessed by MTT assay, and type I collagen secretion was quantified using the enzyme-linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>EndoSequence BC demonstrated significantly greater cell viability than AH Plus (p<0.05). At 48 hours, AH Plus combined with 0.5 μM resveratrol showed significantly greater cell viability than the cement alone and the 0.25 μM resveratrol group (p<0.05). In addition, supplementation with 0.5 μM resveratrol significantly increased type I collagen secretion for both cements compared to the corresponding groups without resveratrol (p<0.05), indicating a positive effect on extracellular matrix production.</p><p><strong>Conclusions: </strong>These findings suggest that resveratrol is a promising additive for improving the bioactivity of endodontic cements, enhancing collagen production and potentially contributing to tissue regeneration and bone repair.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"39 1","pages":"36-42"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Graph Attention and Graph Convolutional Networks for Predicting Candidate SNP-miRNA-DrugInteractions in Integrated Regulatory Datasets.","authors":"Varun Batra, Pradeep K Yadalam, Carlos M Ardila","doi":"10.54589/aol.39/1/43","DOIUrl":"10.54589/aol.39/1/43","url":null,"abstract":"<p><p>Complex genetic and epigenetic factors, such as single-nucleotide polymorphisms (SNPs) that influence the function of microRNAs (miRNAs), are involved in periodontal inflammation and tissue regeneration. However, computational approaches for predicting potential SNP-miRNA interactions that may influence inflammatory signaling and drug responses remain limited in precision periodontology.</p><p><strong>Aim: </strong>To implement and compare two graph neural network-based machine learning models-Graph Attention Network (GAT) and Graph Convolutional Network (GCN)- for predicting potential SNP-miRNA-drug interactions that may be relevant to inflammatory and regenerative pathways associated with periodontal disease.</p><p><strong>Materials and method: </strong>SNPs found in miRNA precursor and target regions associated with immune and drug-response genes were included in three selected miRNA SNP v3.0 database datasets. After preprocessing, composite vectors were created by early fusion of heterogeneous features. Principal component analysis (PCA) was then used to normalize and reduce the composite vectors. Using matched hyperparameters, the GAT and GCN models were built in PyTorch Geometric and trained with cross-entropy loss. Regression metrics (RMSE, MAE, R2) and classification metrics (accuracy, precision, recall, F1-score, ROC-AUC, PR-AUC) were used to evaluate performance.</p><p><strong>Results: </strong>With a perfect recall (1.0), F1-score of 0.666, and PR-AUC of 0.750, GAT performed better in classification than GCN, demonstrating higher sensitivity for identifying candidate interactions within the integrated dataset. Although R2 values were close to zero, indicating limited variance explanation, regression performance was comparable for both models (RMSE ≈ 0.524). Visual diagnostics showed that GAT maintained more prediction variability, while GCN predictions collapsed around mean values.</p><p><strong>Conclusions: </strong>Because of its attention-based architecture, GAT showed improved performance in classification metrics compared with GCN, suggesting potential advantages for prioritizing candidate SNP-miRNA-drug interactions within integrated regulatory datasets. However, the limited predictive performance observed indicates that these findings should be interpreted primarily as a comparative computational evaluation rather than as direct biological evidence. Future studies incorporating periodontalspecific molecular datasets and experimentally validated interactions will be necessary to improve biological interpretability and translational relevance.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"39 1","pages":"43-53"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MRSA carriage rate among dental healthcare personnel: amulticentric cross-sectional study.","authors":"Yirman Adrian García Herrera, Eric Hernández Ochoa, Daniel Hurtado Ortíz, Jorge Orlando Cuellar Mansilla, Martha Jhoanna Arias M, Yeily Isabel Thomas Alvarado, Jesús Arturo Ramirez Sulvaran, Nestor Iván Cardona Perez, Yuly Elien Bernal Rosas, Juana Patricia Sánchez Villamil","doi":"10.54589/aol.39/1/64","DOIUrl":"10.54589/aol.39/1/64","url":null,"abstract":"<p><p>Methicillin-resistant Staphylococcus aureus (MRSA) represents a significant global healthcare challenge, yet its prevalence among dental personnel remains poorly understood.</p><p><strong>Objective: </strong>The aim of this study was to determine the carriage rate and related risk factors of MRSA among Colombian oral healthcare providers.</p><p><strong>Materials and method: </strong>A multicenter descriptive cross-sectional study was conducted. Nasal swabs were obtained from 457 randomly selected healthcare professionals from dental services across eight cities in Colombia, using proportional stratified sampling and snowball sampling in 29% of the cases. Inclusion criteria were being a full time worker in a dentistry setting, and being healthy with no upper respiratory tract infection, fever, or use of antimicrobial therapy in the previous three months. Gram staining, catalase and coagulase tests were used to identify S. aureus colonies. The methicillin-resistant phenotype was detected using the Kirby-Bauer disc method. The virulence factor encoding genes mecA and Panton Valentine Leukocidine (PVL) were detected by polymerase chain reaction (PCR).</p><p><strong>Results: </strong>Overall prevalence of MRSA was 4.4% [95% confidence interval (CI): 2.5% - 6.2%], determined using mecA gene detection. Women carried 75% of the MRSA strains, and dentists accounted for 60%. Carrier rates were 4.03% [95% CI: 1.8% - 6.2%] for dentists, and 5.03% [95% CI: 1.6% - 8.4%] for other personnel. These rates did not differ significantly from each other (Fisher's exact test, p=0.67). A personal history of hospitalization over the previous year was identified as a predisposing factor for the host (p=0.033).</p><p><strong>Conclusion: </strong>This multicenter study reveals a 4.4% MRSA carriage rate among Colombian dental healthcare providers. MRSA-carrying dental healthcare workers did not appear to have risk factors associated with professional activity.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"39 1","pages":"64-73"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compressive strength of endodontically treated mandibularpremolars rehabilitated with three techniques.","authors":"Paula Cebada, Josefina Ingrassia, Eliana G Mamani, Sofía A Pascual, Romina Chaintiou Piorno, Pablo A Martínez, Francisco Somoza, Mariana Picca, Ariel F Gualtieri, Pablo A Rodríguez","doi":"10.54589/aol.39/1/54","DOIUrl":"10.54589/aol.39/1/54","url":null,"abstract":"<p><p>The biomechanical rehabilitation of endodontically treated premolars with extensive mesio-occlusodistal (MOD) cavities is a clinical challenge due to their increased susceptibility to fracture.</p><p><strong>Aim: </strong>To compare the compressive strength of endodontically treated mandibular premolars restored using different post-endodontic reconstruction protocols, using intact teeth as a reference for maximum mechanical performance.</p><p><strong>Materials and method: </strong>Forty single-rooted premolars were distributed into four groups: one control group with no interventions, and three experimental groups with standardized mesio-occluso-distal (MOD) preparations. Experimental groups were restored respectively with core buildup, glass fiber posts, and polyethylene fiber reinforcement. After final composite resin restoration, specimens were tested for compressive strength using an INSTRON 1011 universal testing machine. Data were analyzed using the Kruskal-Wallis test (p<0.05).</p><p><strong>Results: </strong>When examining median (95%CI) compressive strength in Newtons (N), the highest value was found in the control group with 557.4 N (100.8-574.8), followed by the polyethylene fiber group with 471.4 N (194.2 to 565.5), the glass fiber posts group with 427.5 N (146.3 to 495.4) and the core buildup group with 345.1 N (216.8 to 574.9), (p = 0.84).</p><p><strong>Conclusion: </strong>Under the experimental conditions of the present study, the compressive strength of endodontically treated mandibular premolars did not differ significantly among the evaluated reconstruction protocols. Although polyethylene fiber reinforcement showed a tendency toward higher resistance values, the results do not enable the establishment of conclusive mechanical advantages among the restorative systems assessed.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"39 1","pages":"54-63"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of adding graphene oxide to composite resin: An in vitrostudy.","authors":"Beatriz M Árquez Villegas, Carolina Sámano Valencia, Jessica Campos-Delgado, América Paulina Rivera Urbalejo, Erik Reyes Cervantes, Kelly Leite Dos Santos Castro Assis, Julia Flores-Tochihuitl","doi":"10.54589/aol.39/1/3","DOIUrl":"10.54589/aol.39/1/3","url":null,"abstract":"<p><p>Composite resin has been extensively used as a direct restorative material to restore posterior teeth due to its low cost and more complete preservation of the tooth than conventional resin. Nanohybrid resins are a popular alternative due to their apparently better mechanical strength and aesthetic properties compared to conventional resins. However, their clinical performance is still under evaluation. Adding small amounts of graphene oxide (GO) could improve the physicochemical properties of these resins.</p><p><strong>Aim: </strong>The purpose of the current study was to evaluate the effects on the morphological and physicochemical properties of nanohybrid composite resins when GO is added to them.</p><p><strong>Materials and method: </strong>Three kinds of composite resin (Universal Nanohybrid Restorer 3M Filtek Z250 XT) were evaluated: G0, without GO; G1, composite resin with 0.01 % GO; and G2, composite resin with 0.02 % GO. Surface morphology and physicochemical characterization were analyzed for all three groups. Data were submitted to analysis of variance, followed by post hoc Tukey's tests at a significance level of p < 0.05.</p><p><strong>Results: </strong>The addition of GO did not modify the surface of the composite resin compared to the control. The peak of the GO spectrum obtained by FTIR was very small and almost imperceptible due to the close combination of graphene molecules with the rest of the material. Flexural strength increased significantly in group G1, but decreased in G2 compared to the control group. Both GO concentrations resulted in a significant increase in resistance to wear compared to the control group.</p><p><strong>Conclusions: </strong>The addition of graphene oxide did not modify the surface morphology of the nanohybrid compound resin evaluated. However, it did produce changes in mechanical properties: resistance to wear increased significantly at both concentrations studied, while flexural strength changed in a concentration-dependent manner. These results suggest that low concentrations of GO may contribute to improving certain physicochemical properties of nanohybrid compound resins.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"39 1","pages":"3-9"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of disinfection and storage media on surface microhardnessof human enamel blocks.","authors":"Viviana Avila, Carolina Coronel-Ruiz, Karen Daza, Juliana Aguirre, Alejandra Fiesco, Camila Sánchez, Stefania Martignon, Margarita Usuga-Vacca","doi":"10.54589/aol.39/1/74","DOIUrl":"10.54589/aol.39/1/74","url":null,"abstract":"<p><p>Protocolized disinfection and storage are critical to ensuring the quality of dental specimens used in in vitro studies. However, few evaluations have addressed the effect of different combinations of disinfection and storage media on the surface microhardness of human enamel blocks.</p><p><strong>Aim: </strong>To evaluate the effect of different disinfection and storage media on the microhardness of human enamel blocks over four time periods.</p><p><strong>Materials and method: </strong>The study received ethical approval. The surface microhardness of human enamel blocks was measured at baseline (T0). Subsequently, three blocks were assigned to each of six different disinfection-storage media combinations: acetic acid (5%) - chloramine (1%) [AA-CT], hypochlorite (5.25%) - chloramine (1%) [NaOCl-CT], chloramine (0.5%) - chloramine (1%) [CT-CT], acetic acid (5%) - thymol (0.02%) [AA-TH], hypochlorite (5.25%) - thymol (0.02%) [NaOCl-TH], and chloramine (0.5%) - thymol (0.02%) [CT-TH]. After two weeks (T1), one month (T2), three months (T3) or six months (T4) of storage at 4°C, microhardness was reassessed. Percentages of microhardness variation (mineral gain/loss) from baseline were calculated for each time. Data were analyzed using one-way ANOVA and two-way ANOVA to identify differences among disinfection-storage medium combinations and storage times, respectively.</p><p><strong>Results: </strong>A total 72 enamel blocks were analyzed. The microhardness variation percentages showed that the CT-TH combination (T1: 0.00%; T2: 4.05%; T3: 2.78%; T4: -4.74%; p>0.005) had the least variation, and that the microhardness values in the six groups at T3 were the most stable and did not differ significantly (p= 0.630).</p><p><strong>Conclusion: </strong>Combinations of chloramine-thymol and chloramine-chloramine generated the fewest changes in microhardness in enamel blocks, and are suitable for preserving extracted teeth.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"39 1","pages":"74-82"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronology and sequence of permanent tooth eruption in children in Buenos Aires City.","authors":"Marina Andrea Toscano, Jimena Alejandra Anchava, Silvina Gabriela Cortese, Ana María Biondi","doi":"10.54589/aol.39/1/93","DOIUrl":"10.54589/aol.39/1/93","url":null,"abstract":"<p><p>Knowledge of the timing and sequence of permanent tooth eruption is essential for assessing occlusal development and providing population-specific reference standards to support diagnosis, prevention, and treatment planning.</p><p><strong>Aim: </strong>To estimate mean age for eruption of permanent teeth, except third molars, in children in Buenos Aires City (CABA), compare means according to sex, jaw and laterality, and identify the eruption sequence per arch.</p><p><strong>Materials and method: </strong>An observational, prospective, cross-sectional study was conducted (March-July 2024) on 4- to 15-year-old children who received care at Buenos Aires University's School of Dentistry and at four private offices in Buenos Aires City. A tooth was considered erupted when at least one cusp or incisal edge pierced the gingival epithelium. Mean ages were estimated using the Kärber method with 95 % confidence intervals (CI), and the sequence was established by placing those means in order.</p><p><strong>Results: </strong>We evaluated 1,063 children (532 female), mean age 9.9 ± 2.6 years. The first teeth to erupt were lower central incisors, and the last were upper second molars. No difference was observed between hemiarches. Teeth belonging to the lower incisor-canine group erupted significantly earlier than their homologous upper teeth. Girls presented earlier eruption than boys, with significant differences for all teeth except upper central incisors, and upper and lower first molars. Sequence for the upper jaw was 6-1-2-4-3-5-7 in girls, and 6-1-2-4- 5-3-7 in boys. Sequence in the mandible was 1-6-2-3-4-5-7 in girls, and 1-6-2-4-3-5-7 in boys, with chronological proximity between canine and premolars in some comparisons.</p><p><strong>Conclusion: </strong>In this sample, eruption began with lower central incisors, was symmetrical, earlier in girls, and earlier in the lower incisor-canine group, with sequential stability in both sexes during the first transitional period, and variations in the second.</p>","PeriodicalId":93853,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":"39 1","pages":"93-100"},"PeriodicalIF":0.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148868303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}