儿童造血干细胞移植受者唾液巨细胞病毒检测及基因分型的可行性

Sonia P Bohórquez-Ávila, Diana C Álvarez-Correa, Johana Madroñero, Myriam L Velandia-Romero, Jaime E Castellanos
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引用次数: 0

摘要

人巨细胞病毒(HCMV)是一种主要的病毒病原体,可导致免疫抑制个体,特别是造血干细胞移植受者的严重并发症。在这些患者中,巨细胞病毒与胃肠炎、肺炎、肝炎甚至移植物抗宿主病相关,并且已经确定巨细胞病毒基因型与临床病程、并发症和结局之间可能存在关系。早期检测巨细胞病毒感染或再激活是很重要的,先前的研究结果表明,它可能在唾液中进行评估,其中HCMV引起无症状的病毒脱落。由于唾液可以轻松安全地收集,因此评估其在HCMV检测和基因分型方面的潜力非常重要,特别是在接受造血干细胞移植的儿科患者中。目的:本研究的目的是评估在儿童造血干细胞移植受者(HSCTR)队列中使用唾液检测HCMV并进行基因分型的可行性。材料和方法:本研究在哥伦比亚波哥大的Fundación医院Pediátrico la Misericordia进行。每周收集1次刺激唾液样本,定性PCR检测HCMV,巢式PCR分型,测序。最后,构建了系统发育树。结果:共纳入20例患者,收集唾液样本105份,其中HCMV阳性29份。12例患者至少有一个阳性样本。gB1基因型未与其他基因型合并感染。系统发育分析表明,一些唾液样本更接近Towne实验室菌株的序列,而另一些更接近Merlin菌株,两者之间略有差异。结论:研究表明,唾液可用于儿童移植受者巨细胞病毒的检测和基因分型,且样本采集容易,评估的儿童患者无出血或不适的风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Feasibility of using saliva for Cytomegalovirus detection and genotyping in pediatric hematopoietic stem cell transplant recipients.

Feasibility of using saliva for Cytomegalovirus detection and genotyping in pediatric hematopoietic stem cell transplant recipients.

Human Cytomegalovirus (HCMV) is a major viral pathogen that causes severe complications in immunosuppressed individuals, particularly hematopoietic stem cell transplant recipients. In these patients, Cytomegalovirus has been associated with gastroenteritis, pneumonia, hepatitis, and even graft-versus-host disease, and a possible relationship has been identified between Cytomegalovirus genotypes and clinical course, complications and outcome. Early detection of Cytomegalovirus infection or reactivation is important, and previous findings show that it could potentially be evaluated in saliva, where HCMV causes asymptomatic viral shedding. Since saliva can be collected easily and safely, it is important to evaluate its potential for HCMV detection and genotyping, especially in pediatric patients who are receiving hematopoietic stem cell transplantation.

Aim: The purpose of this study was to evaluate the feasibility of using saliva to detect and genotype HCMV in a cohort of pediatric hematopoietic stem cell transplant recipients (HSCTR).

Materials and method: This study was conducted at Fundación Hospital Pediátrico la Misericordia, in Bogota, Colombia. Stimulated saliva samples were collected once a week and subjected to HCMV detection by qualitative PCR and genotyping by nested PCR followed by sequencing. Finally, a phylogenetic tree was constructed.

Results: Twenty patients were enrolled, and 105 saliva samples were collected, of which 29 were positive for HCMV. Twelve patients had at least one positive sample. The gB1 genotype was identified with no coinfection with any other genotype. Phylogenetic analysis showed that some saliva samples were closer to the sequence reported for the Towne laboratory strain, while others were closer to the Merlin strain, with slight differences between them.

Conclusions: It was demonstrated that saliva can be used to detect and genotype Cytomegalovirus in pediatric transplant recipients, and that sample collection is easy, with no risk of bleeding or discomfort in the pediatric patients evaluated.

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