Acta cirurgica brasileira最新文献_第8页

Maturation and development of fetal pig intestinal tissue in immunodeficient mice. 免疫缺陷小鼠胎猪肠道组织的成熟与发育

Acta cirurgica brasileira Pub Date : 2024-02-26 eCollection Date: 2024-01-01 DOI: 10.1590/acb390624

Atsushi Harada, Naoto Matsumoto, Yoshitaka Kinoshita, Kenji Matsu, Yuka Inage, Keita Morimoto, Shuichiro Yamanaka, Masashi Kurobe, Takashi Yokoo, Haruki Kume, Takao Ohki, Eiji Kobayashi

{"title":"Maturation and development of fetal pig intestinal tissue in immunodeficient mice.","authors":"Atsushi Harada, Naoto Matsumoto, Yoshitaka Kinoshita, Kenji Matsu, Yuka Inage, Keita Morimoto, Shuichiro Yamanaka, Masashi Kurobe, Takashi Yokoo, Haruki Kume, Takao Ohki, Eiji Kobayashi","doi":"10.1590/acb390624","DOIUrl":"10.1590/acb390624","url":null,"abstract":"Purpose: This study aimed to compare the degree of maturation and development of fetal pig segmental intestinal tissue with that of spheroids created by in-vitro reaggregation of dissociated fetal intestinal cells after transplantation into immunodeficient mice.Methods: Fetal pig small intestines were transplanted as segmental grafts into the omentum and subrenal capsules of immunodeficient mice or enzymatically treated to generate single cells. Spheroids made by in-vitro reaggregation of these cells were transplanted into the subrenal capsules of immunodeficient mice. The segmental grafts and spheroids were harvested four and eight weeks after transplantation, and the structural maturity and in-vivo development of these specimens were histologically evaluated.Results: The spheroids were engrafted and supplied blood vessels from the host mice, but an intestinal layered structure was not clearly observed, and there was almost no change in size. On the other hand, the segmental grafts formed deep crypts in the mucus membrane, the inner circular layer, and outer longitudinal muscles. The crypts of the transplanted grafts harvested at eight weeks were much deeper, and the smooth muscle layer and the enteric nervous system were more mature than those of grafts harvested at the fourth week, although the intestinal peristaltic wave was not observed.Conclusions: Spheroids created from fetal small intestinal cells could not form layered structures or mature sufficiently. Conversely, segmental tissues structurally matured and developed after in-vivo transplantation and are therefore potential grafts for transplantation.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e390624"},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139998531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical behavior of recurrent pleomorphic adenoma in the palate: a systematic review. 腭部复发性多形性腺瘤的临床表现：系统综述。

Acta cirurgica brasileira Pub Date : 2024-02-26 eCollection Date: 2024-01-01 DOI: 10.1590/acb390824

Gabriela Lopes-Santos, Najara Gomes de Oliveira Marques, Kellen Cristine Tjioe, Denise Tostes Oliveira

{"title":"Clinical behavior of recurrent pleomorphic adenoma in the palate: a systematic review.","authors":"Gabriela Lopes-Santos, Najara Gomes de Oliveira Marques, Kellen Cristine Tjioe, Denise Tostes Oliveira","doi":"10.1590/acb390824","DOIUrl":"10.1590/acb390824","url":null,"abstract":"Purpose: This systematic review analyzed the clinical behavior and odds of malignancy of the palatal recurrent pleomorphic adenomas.Methods: Systematic review of patients with recurrent pleomorphic adenoma arising in the palate. Database search: MEDLINE, Scopus, Web of Science, Cochrane, EMBASE, Virtual Health Library, Google Scholar, and OpenGrey. A binomial logistic regression was performed to assess the odds of detecting recurrence five, 10 and 20 years after the treatment of primary tumor.Results: Thirteen studies (n = 18 patients) out of 336 were included. The recurrent pleomorphic adenoma in palate was more common in females (61.6%), average age was 49 years old (range 9-73 years old). Four patients progressed to malignant transformation. The odds ratio (OR) of detecting a recurrence at 10 (OR = 5.57; 95% confidence interval - 95%CI 1.13-27.52), and 20 years (OR = 18.78; 95%CI 3.18-110.84) after treatment of primary pleomorphic adenoma was significantly higher than at one-year follow-up.Conclusions: The recurrence of pleomorphic adenoma in palate remains a rare event of late occurrence. It mainly affects middle-aged female and carries a risk of malignant transformation. Although uncommon, patients with palatal pleomorphic adenoma should be warned about the possibility of recurrence or malignant transformation of tumor at advanced ages.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e390824"},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139998530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone protective effect of sinomenine against monosodium iodoacetate induced knee and hip injury in rat model: an inflammatory pathway. 西诺明对碘乙酸钠诱导的大鼠膝关节和髋关节损伤的骨保护作用：炎症途径。

Acta cirurgica brasileira Pub Date : 2024-02-05 eCollection Date: 2024-01-01 DOI: 10.1590/acb390924

Yi-Hao Lei, Xing-Xi Hu, Hong-Jie Wen, Yong-Cheng Deng, Jun-Liang Jiang, Qing-Gang Zhao

{"title":"Bone protective effect of sinomenine against monosodium iodoacetate induced knee and hip injury in rat model: an inflammatory pathway.","authors":"Yi-Hao Lei, Xing-Xi Hu, Hong-Jie Wen, Yong-Cheng Deng, Jun-Liang Jiang, Qing-Gang Zhao","doi":"10.1590/acb390924","DOIUrl":"10.1590/acb390924","url":null,"abstract":"Purpose: Osteoarthritis (OA) is a degenerative joint disease which is categorized via destruction of joint cartilage and it also affects the various joints, especially knees and hips. Sinomenine active phytoconstituents isolated from the stem of Sinomenium acutum and already proof anti-inflammatory effect against the arthritis model of rodent. In this experimental protocol, we scrutinized the anti-osteoarthritis effect of sinomenine against monosodium iodoacetate (MIA) induced OA in rats.Methods: MIA (3 mg/50 μL) was used for inducing the OA in the rats, and rats received the oral administration of sinomenine (2.5, 5 and 7.5 mg/kg body weight) up to the end of the experimental study (four weeks). The body and organs weight were estimated. Aggrecan, C-terminal cross-linked telopeptide of type II collagen (CTX-II), glycosaminoglycans (GCGs), monocyte chemoattractant protein-1 (MCP-1), Interferon gamma (IFN-γ), antioxidant, inflammatory cytokines, inflammatory mediators and matrix metalloproteinases (MMP) were analyzed.Results: Sinomenine significantly (P < 0.001) boosted the body weight and reduced the heart weight, but the weight of spleen and kidney remain unchanged. Sinomenine significantly (P < 0.001) reduced the level of nitric oxide, MCP-1 and improved the level of aggrecan, IFN-γ and GCGs. Sinomenine remarkably upregulated the level of glutathione, superoxide dismutase and suppressed the level of malonaldehyde. It effectually modulated the level of inflammatory cytokines and inflammatory mediators and significantly (P < 0.001) reduced the level of MMPs, like MMP-1, 2, 3, 9 and 13.Conclusions: Sinomenine is a beneficial active agent for the treatment of OA disease.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e390924"},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10852535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139704128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined administration of gallic acid and glibenclamide mitigate systemic complication and histological changes in the cornea of diabetic rats induced with streptozotocin. 联合服用没食子酸和格列本脲可减轻全身并发症和链脲佐菌素诱导的糖尿病大鼠角膜的组织学变化。

Acta cirurgica brasileira Pub Date : 2024-02-05 eCollection Date: 2024-01-01 DOI: 10.1590/acb390124

Jing Zhao, Shaik Althaf Hussain, Narendra Maddu

{"title":"Combined administration of gallic acid and glibenclamide mitigate systemic complication and histological changes in the cornea of diabetic rats induced with streptozotocin.","authors":"Jing Zhao, Shaik Althaf Hussain, Narendra Maddu","doi":"10.1590/acb390124","DOIUrl":"10.1590/acb390124","url":null,"abstract":"Purpose: To determine the effect of gallic acid or its combination with glibenclamide on some biochemical markers and histology of the cornea of streptozotocin (STZ) induced diabetic rats.Methods: Following induction of diabetes, 24 male albino rats were divided into four groups of six rats each. Groups 1 and 2 (control and diabetic) received rat pellets and distilled water; group 3 (gallic acid) received rat pellets and gallic acid (10 mg/kg, orally) dissolved in the distilled water; and group 4 (gallic acid + glibenclamide) received rat pellets, gallic acid (10 mg/kg, orally), and glibenclamide (5 mg/kg, orally) dissolved in the distilled water. The treatments were administered for three months after which the rats were sacrificed after an overnight fast. Blood and sera were collected for the determination of biochemical parameters, while their eyes were excised for histology.Results: STZ administration to the rats induced insulin resistance, hyperglycemia, microprotenuria, loss of weight, oxidative stress, inflammation, and alteration of their cornea histology, which was abolished following supplementation with gallic acid or its combination with glibenclamide.Conclusions: The study showed the potentials of gallic acid and glibenclamide in mitigating systemic complication and histological changes in the cornea of diabetic rats induced with STZ.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e390124"},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10852537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139704129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotics prophylaxis at the time of catheter removal after radical prostatectomy: a systematic review of the literature and meta-analysis. 根治性前列腺切除术后拔除导管时的抗生素预防：文献系统回顾和荟萃分析。

Acta cirurgica brasileira Pub Date : 2024-02-05 eCollection Date: 2024-01-01 DOI: 10.1590/acb390424

Letícia Lourenço Dos Santos, Isabela de Almeida Fraga, Vitor Amaral de Almeida, Andressa Hellen Ribeiro Santos, Isabelle Matos Almeida, Tatiana Roberta Nascimento, Breno Cordeiro Porto, Carlo Camargo Passerotti, Everson Luiz de Almeida Artifon, Jose Pinhata Otoch, José Arnaldo Shiomi da Cruz

{"title":"Antibiotics prophylaxis at the time of catheter removal after radical prostatectomy: a systematic review of the literature and meta-analysis.","authors":"Letícia Lourenço Dos Santos, Isabela de Almeida Fraga, Vitor Amaral de Almeida, Andressa Hellen Ribeiro Santos, Isabelle Matos Almeida, Tatiana Roberta Nascimento, Breno Cordeiro Porto, Carlo Camargo Passerotti, Everson Luiz de Almeida Artifon, Jose Pinhata Otoch, José Arnaldo Shiomi da Cruz","doi":"10.1590/acb390424","DOIUrl":"10.1590/acb390424","url":null,"abstract":"Purpose: To conduct a systematic literature review with meta-analysis to identify whether antibiotic prophylaxis after removal of the indwelling urinary catheter reduces posterior infections.Methods: A systematic literature review was conducted in the databases PubMed, Embase, Cochrane, Google Scholar, and Latin American and Caribbean Health Sciences Literature, using the keywords \"antibiotics\" AND \"prostatectomy\" AND \"urinary catheter.\"Results: Three articles were identified having the scope of our review, with 1,040 patients, which were subjected to our meta-analysis revealing a marginally significant decrease in the risk of urinary infection after indwelling urinary catheter removal (odds ratio-OR = 0.51; 95% confidence interval-95%CI 0.27-0.98; p = 0.04; I2 = 0%). No difference was found regarding the presence of bacteriuria (OR = 0.39; 95%CI 0.12-1.24; p = 0.11; I2 = 73%).Conclusions: In our meta-analysis, there was a significant decrease in urinary tract infection with antibiotic prophylaxis after indwelling urinary catheter removal following radical prostatectomy.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e390424"},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10854371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139704127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flap of the sternocephalicus muscle in the repair of a partial defect in the trachea of a rabbit (Oryctolagus cuniculus). 修复兔子气管部分缺损的胸锁乳突肌瓣。

Acta cirurgica brasileira Pub Date : 2024-02-05 eCollection Date: 2024-01-01 DOI: 10.1590/acb390324

Gustavo Fernandes, Ariadne Rein, Gabriel Luiz Montanhim, Marcelo Carrijo da Costa, Marcella Dall'Agnol Leite, Nicolle Pereira Soares, Paola Castro Moraes

{"title":"Flap of the sternocephalicus muscle in the repair of a partial defect in the trachea of a rabbit (Oryctolagus cuniculus).","authors":"Gustavo Fernandes, Ariadne Rein, Gabriel Luiz Montanhim, Marcelo Carrijo da Costa, Marcella Dall'Agnol Leite, Nicolle Pereira Soares, Paola Castro Moraes","doi":"10.1590/acb390324","DOIUrl":"10.1590/acb390324","url":null,"abstract":"Purpose: The current study aimed at evaluating the repair of a partial defect of the trachea with a muscle flap, an advanced technique that employs combined suture patterns.Methods: Sixteen healthy male New Zealand white rabbits were used as an experimental model. A partial defect in the trachea within the ventral region of the fourth to eighth tracheal ring was created. Subsequently, repair was initiated with a flap of the sternocephalicus muscle. The animals were divided into four groups for postoperative evaluation using clinical, tracheoscopic, and histopathological analyses. Each group was separated according to the time of euthanasia, programmed at interval of seven (G7), 15 (G15), 30 (G30), and 60 days (G60).Results: One animal from the G60 group died, whereas the other animals had good surgical recovery without serious changes in the breathing pattern. The major clinical signs observed were stridor and coughing. Tracheoscopy revealed secretions in the tracheal lumen, exuberant granulation, and stenosis. Histopathological analysis showed growth of the ciliary respiratory epithelium at the flap site 30 days after implantation.Conclusions: Partial repair showed satisfactory results owing to the anatomical location of the muscle, adequate vascular support, and structural and physiological maintenance without serious changes in the respiratory system.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e390324"},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10852536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139704131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of rosmarinic acid and doxorubicine on an ovarian adenocarsinoma cell line (OVCAR3) via the EGFR pathway. 迷迭香酸和多柔比星通过表皮生长因子受体途径对卵巢腺癌细胞系（OVCAR3）的影响

Acta cirurgica brasileira Pub Date : 2024-02-05 eCollection Date: 2024-01-01 DOI: 10.1590/acb390524

Umut Sarı, Fuat Zaman

{"title":"Effects of rosmarinic acid and doxorubicine on an ovarian adenocarsinoma cell line (OVCAR3) via the EGFR pathway.","authors":"Umut Sarı, Fuat Zaman","doi":"10.1590/acb390524","DOIUrl":"10.1590/acb390524","url":null,"abstract":"Purpose: We aimed to reveal the effects of rosmarinic acid (RA), which has come to the forefront with its antitumor and antioxidant properties in many studies recently in the ovarian adenocarcinoma cell line, on the epidermal growth factor receptor (EFGR) signaling pathway in the presence of doxorubicin (DOX).Methods: Ovarian adenocarcinoma cell line (OVCAR3) and human skin keratinocyte cell line human skin keratinocyte cell line (HaCaT) were used as control. (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test was applied to determine the effect of RA and DOX on the proliferation of OVCAR3 and HaCaT cells. Bcl2 expression and epidermal growth factor receptor (EGFR) and western blot analysis were performed to determine the expression levels of the markers.Results: It was determined that RA (IC50 = 437.6 μM) and DOX (IC50 = 0.08 μM) have the ability to inhibit the proliferation of OVCAR3 cells and induce apoptosis in a 72-hour time and dose-dependent manner. Western blot showed that the expression level of Bcl-2 and EGFR in OVCAR3 cells was down-regulated by RA and DOX.Conclusions: Apoptosis in OVCAR3 cells can potentially be induced by RA via the EGFR pathway, and RA may be a potent agent for cancer therapy.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e390524"},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10852540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139704130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of chronic restraint stress in the prostate of prepubertal and adult rats. 慢性抑制性应激对青春期前和成年大鼠前列腺的影响。

Acta cirurgica brasileira Pub Date : 2023-12-04 eCollection Date: 2023-01-01 DOI: 10.1590/acb387123

Isabella Mendes Procópio, Carina Teixeira Ribeiro, Roger Gaspar Marchon, Waldemar Silva Costa, Gabriela Faria Buys-Gonçalves, Francisco José Barcellos Sampaio, Marco Aurélio Pereira-Sampaio, Diogo Benchimol de Souza

{"title":"Effects of chronic restraint stress in the prostate of prepubertal and adult rats.","authors":"Isabella Mendes Procópio, Carina Teixeira Ribeiro, Roger Gaspar Marchon, Waldemar Silva Costa, Gabriela Faria Buys-Gonçalves, Francisco José Barcellos Sampaio, Marco Aurélio Pereira-Sampaio, Diogo Benchimol de Souza","doi":"10.1590/acb387123","DOIUrl":"10.1590/acb387123","url":null,"abstract":"Purpose: To investigate the effects of chronic stress in the prostate of prepubertal and adult rats.Methods: Thirty-two male rats were assigned into four groups depending on the type of treatment (control or stressed) and the age at which stress was initiated (prepubertal or adult). Restraint stress stimuli were applied for six weeks. Stressed prepubertal and adult rats evaluated immediately after the last stress stimuli were named SP and SA groups, respectively. Age-matched rats were used as control groups (CP and CA). At the end of the experiment, the rats were euthanized, and prostate morphological parameters were evaluated and statistically compared.Results: Application of stress stimuli to the SP group resulted in reduced body weight, but no prostate morphological modification was noted. The SA group showed reduced testosterone level and prostatic epithelium surface density, in comparison to CA group. Further, the prostatic lumen surface density was increased in adult stressed animals, in comparison to adult controls.Conclusions: The stress stimuli promoted changes in hormonal and morphological parameters in the prostate of adult stressed rats. Prepubertal stressed animals did not presented modifications of prostate morphology.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e387123"},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10695186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Are both distinct epithelial and stromal cells molecular analysis from phyllodes tumors versus fibroadenoma components affected in breast fibroepithelial progression? 叶状瘤和纤维腺瘤成分的不同上皮细胞和基质细胞分子分析是否影响乳腺纤维上皮的进展?

Acta cirurgica brasileira Pub Date : 2023-12-04 eCollection Date: 2023-01-01 DOI: 10.1590/acb386823

Ângela Flavia Logullo Waitzberg, Elisa Napolitano E Ferreira, Mabel Pinilla, Paulo Pineda, Andréa Cristina de Moraes Malinverni, Fernando Augusto Soares, Dirce Maria Carraro

{"title":"Are both distinct epithelial and stromal cells molecular analysis from phyllodes tumors versus fibroadenoma components affected in breast fibroepithelial progression?","authors":"Ângela Flavia Logullo Waitzberg, Elisa Napolitano E Ferreira, Mabel Pinilla, Paulo Pineda, Andréa Cristina de Moraes Malinverni, Fernando Augusto Soares, Dirce Maria Carraro","doi":"10.1590/acb386823","DOIUrl":"10.1590/acb386823","url":null,"abstract":"Purpose: To determine molecular events involved in the tumorigenesis of phyllodes tumors (PT) and the role of each stromal (SC) and epithelial (EC) cell.Methods: Frozen breast samples enriched with epithelial and stromal cells from three fibroadenomas and 14 PT were retrieved and laser microdissected. Sanger and polymerase chain reaction-based sequencing of exon 2 MED12 and TERT promoter hotspot mutations were performed; 44K microarray platform was used to analyze gene expression.Results: All three fibroadenomas (FAs) presented mutations in MED12, but not in TERT, whose mutation was observed in five of the 14 PTs. EC and SC of each affected tumor displayed identical alterations. Of the total differentially expressed genes (DEG) (EC = 1,543 and SC = 850), 984 were EC-eDEGs and 291 were SC-eDEGs. We found a high similarity of diseases and functions enriched by both cell types, but dissimilarity in the number of enriched canonical pathways. Three signaling canonical pathways overlapping with EC and SC were predicted to be activated in one cell type and inactivated in the other, while no overlap in eDEGs was assigned to them. We also identified 13 EC-eDEGs and five SC-eDEGs enriched networks, in which the SC-eDEGs were able to segregate FA from PT samples.Conclusions: Identical TERT mutations from both SC and ES origins might affect the PTs tumorigenesis. Gene expression differences suggest coordinated molecular processes between these components with determinant differences acquired by SC, able to fully distinguish PTs from FAs lesions.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e386823"},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10695188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing financing models for supplementary healthcare in appendectomy: activity-based costing (fee-for-service) vs. diagnosis related group remuneration (bundled payment) - a systematic review and meta-analysis. 比较阑尾切除术补充医疗的融资模式:基于活动的成本核算(按服务收费)与诊断相关的团体报酬(捆绑支付)——一项系统回顾和荟萃分析。

Acta cirurgica brasileira Pub Date : 2023-12-04 eCollection Date: 2023-01-01 DOI: 10.1590/acb386923

André de Arimatéia de Souza Lino, Jose Arnaldo Shiomi da Cruz, Breno Cordeiro Porto, Rhuan Pimentel Nogueira, José Pinhata Otoch, Everson Luiz de Almeida Artifon

{"title":"Comparing financing models for supplementary healthcare in appendectomy: activity-based costing (fee-for-service) vs. diagnosis related group remuneration (bundled payment) - a systematic review and meta-analysis.","authors":"André de Arimatéia de Souza Lino, Jose Arnaldo Shiomi da Cruz, Breno Cordeiro Porto, Rhuan Pimentel Nogueira, José Pinhata Otoch, Everson Luiz de Almeida Artifon","doi":"10.1590/acb386923","DOIUrl":"10.1590/acb386923","url":null,"abstract":"Purpose: In Brazil, healthcare services traditionally follow a fee-for-service (FFS) payment system, in which each medical procedure incurs a separate charge. An alternative reimbursement with the aim of reducing costs is diagnosis related group (DRG) remuneration, in which all patient care is covered by a fixed amount. This work aimed to perform a systematic review followed by meta-analysis to assess the effectiveness of the Budled Payment for Care Improvement (BPCI) versus FFS.Methods: Our work was performed following the items of the PRISMA report. We included only observational trials, and the primary outcome assessed was the effectiveness of FFS and DRG in appendectomy considering complications. We also assessed the costs and length of hospital stay. Meta-analysis was performed with Rev Man version 5.4.Results: Out of 735 initially identified articles, six met the eligibility criteria. We demonstrated a shorter hospital stay associated with the DRG model (mean difference = 0.39; 95% confidence interval - 95%CI - 0.38-0.40; p < 0.00001; I2 = 0%), however the hospital readmission rate was higher in this model (odds ratio = 1.57; 95%CI 1.02-2.44, p = 0.04; I2 = 90%).Conclusions: This study reveals a potential decrease in the length of stay for appendectomy patients using the DRG approach. However, no significant differences were observed in other outcomes analysis between the two approaches.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e386923"},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10699206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0