Calendula glycolic extract enhances wound healing of alginate hydrogel.

Acta cirurgica brasileira Pub Date : 2024-11-29 eCollection Date: 2024-01-01 DOI:10.1590/acb399724

Gisele de Oliveira Krubniki Possa, Solange Chopek, Airton Vicente Pereira, Adriana Yuriko Koga, Marcia Regina Paes de Oliveira, Michele Dietrich Moura Costa

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Abstract

Purpose: To assess the cytotoxicity and wound healing properties of an alginate hydrogel containing calendula glycolic extract.

Methods: Cell viability in murine fibroblasts (3T3 cells) was evaluated using MTT and SRB assays. The wound healing effect was tested in an incisional wound model on 50 female Wistar rats, divided into two groups: rats treated with alginate hydrogel (n = 25), and rats treated with calendula-alginate hydrogel. Wound healing was assessed by measuring wound retraction rate and histological analysis of lesion tissues over a 28-day period. Histological analyses were performed on days 2, 7, 14, 21, and 28 post-surgery to examine inflammatory infiltrate, macrophage count, and angiogenesis. Picrosirius red staining was used to compare the relative abundance of collagen types I and III fibers.

Results: Cytotoxicity tests on 3T3 cells revealed increased cell viability with the calendula-alginate hydrogel. The calendula-alginate hydrogel also demonstrated a significant improvement in wound closure, supported by histopathological analysis, showing reduced inflammation, increased macrophage activity, and enhanced collagen deposition.

Conclusions: These findings evidenced the therapeutic potential of combining calendula extract and alginate for promoting enhanced wound healing.

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金盏花乙醇酸提取物促进伤口愈合海藻酸盐水凝胶。

目的：评价含有金盏花乙醇酸提取物的海藻酸盐水凝胶的细胞毒性和伤口愈合性能。方法：采用MTT法和SRB法测定小鼠成纤维细胞（3T3细胞）的细胞活力。采用50只雌性Wistar大鼠建立切口创面模型，观察创面愈合效果，将其分为两组：海藻酸盐水凝胶组（n = 25）和金盏花-海藻酸盐水凝胶组（n = 25）。在28天的时间里，通过测量伤口缩回率和损伤组织的组织学分析来评估伤口愈合情况。在术后第2、7、14、21和28天进行组织学分析，检查炎症浸润、巨噬细胞计数和血管生成。用小天狼星红染色比较I型和III型胶原纤维的相对丰度。结果：对3T3细胞的细胞毒性试验显示，金盏花-海藻酸盐水凝胶提高了细胞活力。组织病理学分析显示，金盏花-海藻酸盐水凝胶在伤口愈合方面也有显着改善，显示炎症减少，巨噬细胞活性增加，胶原沉积增强。结论：这些发现证明了金盏花提取物和海藻酸盐联合使用促进伤口愈合的治疗潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta cirurgica brasileira

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