JSFA reports最新文献

筛选
英文 中文
Applications of Curcumin Loaded Nanoemulsions in Photoinactivation of A. Niger spores 姜黄素负载纳米乳液在光灭活A.Niger孢子中的应用
JSFA reports Pub Date : 2023-05-25 DOI: 10.1002/jsf2.134
J. A. Arboleda‐Murillo, Andrés F. Cañon-Ibarra, Leidy T. Sanchez, M. I. Pinzón, Cristian C. Villa
{"title":"Applications of Curcumin Loaded Nanoemulsions in Photoinactivation of\u0000 A. Niger\u0000 spores","authors":"J. A. Arboleda‐Murillo, Andrés F. Cañon-Ibarra, Leidy T. Sanchez, M. I. Pinzón, Cristian C. Villa","doi":"10.1002/jsf2.134","DOIUrl":"https://doi.org/10.1002/jsf2.134","url":null,"abstract":"","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47671390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dietary exposure and risk assessment of polychlorinated biphenyls to artisanal fishermen and fish vendors: A case study of Dar es Salaam 个体渔民和鱼贩对多氯联苯的饮食暴露和风险评估:以达累斯萨拉姆为例
JSFA reports Pub Date : 2023-05-25 DOI: 10.1002/jsf2.133
Gabriel Jacob Gabriel Mwaijibe, Beatrice Mgaya Kilima
{"title":"Dietary exposure and risk assessment of polychlorinated biphenyls to artisanal fishermen and fish vendors: A case study of Dar es Salaam","authors":"Gabriel Jacob Gabriel Mwaijibe,&nbsp;Beatrice Mgaya Kilima","doi":"10.1002/jsf2.133","DOIUrl":"https://doi.org/10.1002/jsf2.133","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Fish is one of the main sources of food and income in Tanzania, with 25% of the population depending on fishing activities as a source of food, income, and employment. But fish consumers are vulnerable to exposure to toxic pollutants including PCBs due to the lipophilic nature of the pollutants which accumulate in fish.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The estimated mean NDL PCBs concentration level was 0.84 ng/g w/w and the mean fish consumption rate among fishermen was 0.4 kg/day. Mean exposure of the fishermen and fish vendors to total PCBs ranged between 9.62 and 36.12 ng/kg bw/day depending on the estimation scenario (lower bound/upper bound) for both normal and higher fish consumers. The general population exposure to total PCBs was 0.4 and 0.8 ng/kg bw/day in lower-bound and upper-bound estimation scenarios respectively. The estimated lifetime carcinogenic and non-carcinogenic hazard indices from intake of estimated total PCBs by fishermen and fish vendors exceeded the recommended tolerable daily intake, TDI, raising serious concerns for adverse health effects to fishermen and fish vendors. However, for the general population, there was no concern for any potential health effects as the estimated hazard indices were far below the recommended levels for intake of total PCBs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The exposure levels of fishermen and fish vendors to PCBs via consumption of <i>Rastrelliger kanagutra</i> (Indian mackerel) in Dar es Salaam are high enough to course serious health concerns, especially for high fish consumers and efforts should be taken to encourage diversification of fish species intake.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 6","pages":"282-289"},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50154299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evolution of the quality and sensory characteristics of extra virgin olive oil as affected by phenolic content during long-time storage at room temperature 在室温下长时间储存期间,受酚含量影响的特级初榨橄榄油质量和感官特性的演变
JSFA reports Pub Date : 2023-05-25 DOI: 10.1002/jsf2.132
Natália Cristina Averbuch, Lucas Tolio Silva, Luana Souza Cavalcante, Isabel Cristina Kasper Machado, Sara Oberlaender dos Santos, Júlia Leão Nogueira, Camila Scheid, Josias Merib, Juliano Garavaglia
{"title":"Evolution of the quality and sensory characteristics of extra virgin olive oil as affected by phenolic content during long-time storage at room temperature","authors":"Natália Cristina Averbuch,&nbsp;Lucas Tolio Silva,&nbsp;Luana Souza Cavalcante,&nbsp;Isabel Cristina Kasper Machado,&nbsp;Sara Oberlaender dos Santos,&nbsp;Júlia Leão Nogueira,&nbsp;Camila Scheid,&nbsp;Josias Merib,&nbsp;Juliano Garavaglia","doi":"10.1002/jsf2.132","DOIUrl":"10.1002/jsf2.132","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Extra Virgin olive oils are directly obtained from olives and their quality is influenced by the oxidative process during storage as consequence the category may be penalized. Sensory attributes are crucial parameter of olive oils quality and classifications. This study evaluated the main sensorial and volatile profile of three olive oils with high medium and low content of initial total phenolic compounds (from ‘Koroneiki’ Blend of varieties and ‘Arbequina’ respectively) including quality parameters up to 15 months of storage.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A significant increase of peroxide index value (around 50%) and reduction of phenolic compounds level (around 60%) were observed. The sensory attributes of sweet herbaceous almond and fruity notes decreased and the majority of volatile compounds found in all olive oils were (E)-2-hexenal (Z)-3-hexen-1-ol 1-penten-3-one and hexanal even after 15 months. The reductions of different parameters are related to initial phenolic level and samples are classified as Virgin after seven (low phenolics) nine (medium phenolics) and 11 (high phenolics) months displaying defect of rancid in distinct intensities.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This investigation provided detailed about Brazilian olive oils useful in a global context moreover modifications of sensory and volatile compounds profile and its evolution according to the long-time storage were established.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 7","pages":"320-330"},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49132240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of adaptation length on assayed metabolizable energy of maize and barley supplemented with or without glucanase for broiler chickens using the index method 指数法测定玉米和大麦添加或不添加葡聚糖酶对肉鸡代谢能的影响
JSFA reports Pub Date : 2023-05-25 DOI: 10.1002/jsf2.135
Shravani Veluri, Oluyinka A. Olukosi
{"title":"Influence of adaptation length on assayed metabolizable energy of maize and barley supplemented with or without glucanase for broiler chickens using the index method","authors":"Shravani Veluri,&nbsp;Oluyinka A. Olukosi","doi":"10.1002/jsf2.135","DOIUrl":"https://doi.org/10.1002/jsf2.135","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>This effect of adaptation length (AL) on the apparent metabolizable energy (AME) and nitrogen-corrected AME (AMEn) of maize and barley supplemented with or without β-glucanase enzyme was assessed in this study. Three experimental diets were formulated with a wheat-soybean meal diet as a reference diet (RD) and two additional diets with maize or barley included at 300 g kg<sup>−1</sup> into the reference diet. A total of 324 Cobb 500 broilers were used for the experiment in a 3 × 2 factorial with factors AL (10, 7, or 4 days) and enzyme (with or without) for cereal grains maize and barley. Birds on 10, 7, or 4 days AL were given experimental diets beginning at from d 11, 14, and 17 of age, respectively. Excreta were collected on d 20 and 21. The AME and AMEn of cereal grain were calculated by the difference method.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>There was no significant interaction between the factors for barley AME and AMEn. The AME and AMEn of maize decreased <i>p</i> &lt; 0.05) step-wise with increasing in AL from 4 to 10 days. However, when supplemented with an enzyme, the AME and AMEn of maize were similar at 4, 7, and 10 days AL. Enzyme supplementation improved (<i>p</i> &lt; 0.01) AME and AMEn of barley, but there was no effect of AL on barley AME and AMEn.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>In conclusion, the influence of AL on assayed AME in cereal grains supplemented with enzyme was more pronounced for maize and less so for barley.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 7","pages":"304-309"},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50143885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dietary exposure and risk assessment of polychlorinated biphenyls ( PCBs ) to artisanal fishermen and fish vendors. A case study of Dar es Salaam 个体渔民和鱼类供应商对多氯联苯的饮食接触和风险评估。达累斯萨拉姆案例研究
JSFA reports Pub Date : 2023-05-25 DOI: 10.1002/jsf2.133
Gabriel Jacob Gabriel Mwaijibe, B. Kilima
{"title":"Dietary exposure and risk assessment of polychlorinated biphenyls (\u0000 PCBs\u0000 ) to artisanal fishermen and fish vendors. A case study of Dar es Salaam","authors":"Gabriel Jacob Gabriel Mwaijibe, B. Kilima","doi":"10.1002/jsf2.133","DOIUrl":"https://doi.org/10.1002/jsf2.133","url":null,"abstract":"","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45278815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Volatile components of Pelaverga red wines from Piedmont (Italy) 意大利皮埃蒙特佩拉韦加红葡萄酒挥发性成分分析
JSFA reports Pub Date : 2023-05-18 DOI: 10.1002/jsf2.129
Elisabetta Bonometti, Lorenza Operti, Roberto Rabezzana, Giorgio Volpi
{"title":"Volatile components of Pelaverga red wines from Piedmont (Italy)","authors":"Elisabetta Bonometti,&nbsp;Lorenza Operti,&nbsp;Roberto Rabezzana,&nbsp;Giorgio Volpi","doi":"10.1002/jsf2.129","DOIUrl":"10.1002/jsf2.129","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The characterization of a typical red wine from Piedmont (north-west Italy), Pelaverga, was performed by means of headspace solid phase microextraction coupled to gas chromatography–mass spectrometry. Two different kinds of Pelaverga are known, hystorical and Verduno, which differ with the area of production. The main volatile compounds of the wine were identified and semi-quantified. Principal components analysis (PCA) was performed to identify possible clusters among the different samples analyzed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Forty-eight volatile compounds were unambiguously identified in the wines sampled. The two types of Pelaverga analyzed displayed a very similar volatile profile. The most abundant volatile compounds were two esters, ethyl octanoate and diethyl succinate, and two alcohols, isoamyl alcohol and ethyl phenyl alcohol. Few varietal compounds were detected, probably due to their low concentration; among these, we were not able to detect rotundone, which is reported to impart characteristic spicy notes to this kind of wine. Application of PCA did not allow to differentiate between the two types of Pelaverga on the basis of their volatile profile.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The present work is, to the best of our knowledge, the first complete report about the volatile profile of Pelaverga wines and represents the first step of a research project aiming at characterizing food products from Piedmont on the basis of chemical parameters. Differentiation between the two types of Pelaverga will likely require the determination of different chemical parameters in conjunction with volatile compounds.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 6","pages":"262-270"},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47315575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of spray drying conditions on physicochemical and functional properties of apple cider vinegar powder 喷雾干燥条件对苹果醋粉理化性能和功能性能的影响
JSFA reports Pub Date : 2023-05-18 DOI: 10.1002/jsf2.130
Ipek Altay, Peter Reimer Stubbe, Mohammad Amin Mohammadifar
{"title":"Effect of spray drying conditions on physicochemical and functional properties of apple cider vinegar powder","authors":"Ipek Altay,&nbsp;Peter Reimer Stubbe,&nbsp;Mohammad Amin Mohammadifar","doi":"10.1002/jsf2.130","DOIUrl":"10.1002/jsf2.130","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Apple cider vinegar (ACV) contains many health benefits due to its antioxidant and acetic acid content. However, the adverse effects of directly consuming the vinegar should be eliminated to make it possible to regulate its intake as a dietary supplement. The objective of the study is to optimize the spray drying operating conditions to produce ACV powder with health benefits. A central composite design (CCD) of response surface methodology (RSM) was used to optimize the spray drying process of ACV. The influences of inlet temperature (130°C–170°C), gum Arabic concentration (5%–15% w/w), and feed flow rate (1–2 kg/h) on some product and process aspects were investigated. Spray-dried powders were evaluated for their moisture content, process yield, acetic acid content, solubility, and antioxidant content.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The relationships between the model factors and final powder properties were established with ANOVA and multiple regression analysis. The model factors affected the investigated powder properties at different significance levels, where carrier concentration was the main influencing factor. Gum Arabic contributed to the retention of volatile and heat-sensitive compounds providing health benefits. All powders showed high solubility (&gt;94%) and high antioxidant recovery, and most treatments achieved a drying yield of higher than 50%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Optimization of spray drying process was achieved with application of RSM and relationships between model factors and powder properties were established. This study highlighted the feasibility of the development of ACV-based functional powder as a dietary supplement.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 6","pages":"271-281"},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48286168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of cyanide intake through consumption of cassava-based diets in the Democratic Republic of Congo 在刚果民主共和国,通过食用以木薯为基础的饮食来评估氰化物摄入量
JSFA reports Pub Date : 2023-05-18 DOI: 10.1002/jsf2.131
Christus C. Miderho, Lucy G. Njue, George O. Abong'
{"title":"Assessment of cyanide intake through consumption of cassava-based diets in the Democratic Republic of Congo","authors":"Christus C. Miderho,&nbsp;Lucy G. Njue,&nbsp;George O. Abong'","doi":"10.1002/jsf2.131","DOIUrl":"10.1002/jsf2.131","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Konzo is attributed to inadequate cassava processing and consumption of cassava meals with little protein, which is characterized by a neurological disorder that causes paralysis of the lower limbs. However, the exposure to cyanide in the Mwana health zone is still unknown while the prevalence of konzo is assumed to be high compared with other areas in the Democratic Republic of Congo (DRC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The results showed that women are the most affected (56.7%), followed by young people under the age of 10 (27%), and people from low socioeconomic class at 93.4%. The prevalence of konzo in Burhinyi is 0.06%. Cyanide intake by the population of Burhinyi ranged from 26 to 271 mg/kg bw/day at the 95th percentile through consumption of cassava-based <i>ugali</i>. Margin of exposure was around 0.271 and 0.393 g/person/day.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Poor people, women, and children are more at risk for konzo disease due to a diet dominated by under processed food made from cassava. However, since 2011, the study area's konzo prevalence has remained constant.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 7","pages":"310-319"},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41559821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Approaches to check unauthorized genetically modified events in supply chain: Challenges and solutions in the Indian context 检查供应链中未经授权的转基因事件的方法:印度背景下的挑战和解决方案
JSFA reports Pub Date : 2023-04-19 DOI: 10.1002/jsf2.115
Monika Singh, Nina Papazova, Raghavendra Aminedi, Marie-Alice Fraiture, Nancy Roosens, Gurinderjit Randhawa
{"title":"Approaches to check unauthorized genetically modified events in supply chain: Challenges and solutions in the Indian context","authors":"Monika Singh,&nbsp;Nina Papazova,&nbsp;Raghavendra Aminedi,&nbsp;Marie-Alice Fraiture,&nbsp;Nancy Roosens,&nbsp;Gurinderjit Randhawa","doi":"10.1002/jsf2.115","DOIUrl":"10.1002/jsf2.115","url":null,"abstract":"<p>The approval status of a genetically modified (GM) event varies from country to country. GM events approved in one country (considered as authorized GM or AGM) may not necessarily have the same approval status in other countries (considered as unauthorized GM or UGM). Detecting UGM in the supply chain is a challenge as the genetic information is not always available. In India, four <i>Bt</i> cotton events are approved, whereas several other GM events have been imported for research purposes. Many food derivatives (non-GM) are being imported from the countries where GM events of food crops are approved so it is necessary to track the unauthorized entry of GM products. Selected consignments or food products need to be checked for GM status for regulatory compliance. In farmers' fields, the chances of unintentional introgression or adventitious presence of transgenes also need to be monitored in a systematic manner. An appropriate strategy needs to be developed to check for UGM in the food and agricultural supply chain. In this article, approaches for UGM detection have been discussed with a focus on application in the Indian context. Detection methods based on the GMO matrix, multiplex PCR, real-time PCR (qPCR), and loop-mediated isothermal amplification (LAMP) could be employed keeping in view the regulatory requirement or practical application. For checking UGM with unknown genetic construct, methods such as next-generation sequencing (NGS) may be employed. The advantages and disadvantages of the different approaches are discussed in the function of the analytical strategy and its application for control purposes.</p>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 5","pages":"184-195"},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43757779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biotive protease inhibitor from leaves of Capsicum annuum L.: Potential use as an antifungal molecule against Colletotrichum scovillei 辣椒叶片中的生物蛋白酶抑制剂:作为抗炭疽菌分子的潜在用途
JSFA reports Pub Date : 2023-04-19 DOI: 10.1002/jsf2.116
Lídia S. Pereira, Gabriel B. Taveira, Thaynã A. M. Souza, André O. Carvalho, Virginia S. Carvalho, Rosana Rodrigues, Katia Valevski S. Fernandes, Erica O. Mello, Valdirene M. Gomes
{"title":"Biotive protease inhibitor from leaves of Capsicum annuum L.: Potential use as an antifungal molecule against Colletotrichum scovillei","authors":"Lídia S. Pereira,&nbsp;Gabriel B. Taveira,&nbsp;Thaynã A. M. Souza,&nbsp;André O. Carvalho,&nbsp;Virginia S. Carvalho,&nbsp;Rosana Rodrigues,&nbsp;Katia Valevski S. Fernandes,&nbsp;Erica O. Mello,&nbsp;Valdirene M. Gomes","doi":"10.1002/jsf2.116","DOIUrl":"10.1002/jsf2.116","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>A diverse set of antimicrobial molecules have been found mainly in the seeds and fruits of <i>Capsicum annuum</i> UENF1381, accession resistant to different phytopathogenic microorganisms, like bacteria of the genus <i>Xanthomonas</i> and fungi of the genus <i>Colletotrichum</i>. However, there is little information about the defense mechanisms in other plant organs. The work aimed to study the induction of resistance in leaves and roots of <i>C</i>. <i>annuum</i> through the identification and characterization of antimicrobial peptides against <i>Colletotrichum scovillei</i>, one of the main pathogens of the crop.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Based on the results of differential protein expression in response to infection, the extracts, LC<sub>48</sub> (Control Leaf mock inoculated 48 h), and RC<sub>48</sub> (Control Root mock inoculated 48 h) were selected. Analysis on the HPLC system resulted in 23 fractions (L1 to L23) obtained from the LC<sub>48</sub> extract and 27 fractions from the RC<sub>48</sub> extract (R1 to R27). Our results show that 10 μg mL<sup>−1</sup> of the L1 fraction is able to inhibit 88.4% of fungal growth, causing cell membrane permeabilization, endogenous reactive oxygen species induction and mitochondrial activity reduction. The peptide in the L1 fraction, named <i>Ca</i>LPI, showed similarities with different sequences of proteinase inhibitors.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The study provides information on the diversity of antimicrobial proteins and peptides present in the leaves and roots of <i>C</i>. <i>annuum</i> UENF1381. With these results, we hope to contribute to the use of peptides as potential molecules in microbial control.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 5","pages":"248-257"},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43549057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信