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Influence of adaptation length on assayed metabolizable energy of maize and barley supplemented with or without glucanase for broiler chickens using the index method 指数法测定玉米和大麦添加或不添加葡聚糖酶对肉鸡代谢能的影响
JSFA reports Pub Date : 2023-05-25 DOI: 10.1002/jsf2.135
Shravani Veluri, Oluyinka A. Olukosi
{"title":"Influence of adaptation length on assayed metabolizable energy of maize and barley supplemented with or without glucanase for broiler chickens using the index method","authors":"Shravani Veluri,&nbsp;Oluyinka A. Olukosi","doi":"10.1002/jsf2.135","DOIUrl":"https://doi.org/10.1002/jsf2.135","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>This effect of adaptation length (AL) on the apparent metabolizable energy (AME) and nitrogen-corrected AME (AMEn) of maize and barley supplemented with or without β-glucanase enzyme was assessed in this study. Three experimental diets were formulated with a wheat-soybean meal diet as a reference diet (RD) and two additional diets with maize or barley included at 300 g kg<sup>−1</sup> into the reference diet. A total of 324 Cobb 500 broilers were used for the experiment in a 3 × 2 factorial with factors AL (10, 7, or 4 days) and enzyme (with or without) for cereal grains maize and barley. Birds on 10, 7, or 4 days AL were given experimental diets beginning at from d 11, 14, and 17 of age, respectively. Excreta were collected on d 20 and 21. The AME and AMEn of cereal grain were calculated by the difference method.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>There was no significant interaction between the factors for barley AME and AMEn. The AME and AMEn of maize decreased <i>p</i> &lt; 0.05) step-wise with increasing in AL from 4 to 10 days. However, when supplemented with an enzyme, the AME and AMEn of maize were similar at 4, 7, and 10 days AL. Enzyme supplementation improved (<i>p</i> &lt; 0.01) AME and AMEn of barley, but there was no effect of AL on barley AME and AMEn.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>In conclusion, the influence of AL on assayed AME in cereal grains supplemented with enzyme was more pronounced for maize and less so for barley.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 7","pages":"304-309"},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50143885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dietary exposure and risk assessment of polychlorinated biphenyls ( PCBs ) to artisanal fishermen and fish vendors. A case study of Dar es Salaam 个体渔民和鱼类供应商对多氯联苯的饮食接触和风险评估。达累斯萨拉姆案例研究
JSFA reports Pub Date : 2023-05-25 DOI: 10.1002/jsf2.133
Gabriel Jacob Gabriel Mwaijibe, B. Kilima
{"title":"Dietary exposure and risk assessment of polychlorinated biphenyls (\u0000 PCBs\u0000 ) to artisanal fishermen and fish vendors. A case study of Dar es Salaam","authors":"Gabriel Jacob Gabriel Mwaijibe, B. Kilima","doi":"10.1002/jsf2.133","DOIUrl":"https://doi.org/10.1002/jsf2.133","url":null,"abstract":"","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45278815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Volatile components of Pelaverga red wines from Piedmont (Italy) 意大利皮埃蒙特佩拉韦加红葡萄酒挥发性成分分析
JSFA reports Pub Date : 2023-05-18 DOI: 10.1002/jsf2.129
Elisabetta Bonometti, Lorenza Operti, Roberto Rabezzana, Giorgio Volpi
{"title":"Volatile components of Pelaverga red wines from Piedmont (Italy)","authors":"Elisabetta Bonometti,&nbsp;Lorenza Operti,&nbsp;Roberto Rabezzana,&nbsp;Giorgio Volpi","doi":"10.1002/jsf2.129","DOIUrl":"10.1002/jsf2.129","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The characterization of a typical red wine from Piedmont (north-west Italy), Pelaverga, was performed by means of headspace solid phase microextraction coupled to gas chromatography–mass spectrometry. Two different kinds of Pelaverga are known, hystorical and Verduno, which differ with the area of production. The main volatile compounds of the wine were identified and semi-quantified. Principal components analysis (PCA) was performed to identify possible clusters among the different samples analyzed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Forty-eight volatile compounds were unambiguously identified in the wines sampled. The two types of Pelaverga analyzed displayed a very similar volatile profile. The most abundant volatile compounds were two esters, ethyl octanoate and diethyl succinate, and two alcohols, isoamyl alcohol and ethyl phenyl alcohol. Few varietal compounds were detected, probably due to their low concentration; among these, we were not able to detect rotundone, which is reported to impart characteristic spicy notes to this kind of wine. Application of PCA did not allow to differentiate between the two types of Pelaverga on the basis of their volatile profile.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The present work is, to the best of our knowledge, the first complete report about the volatile profile of Pelaverga wines and represents the first step of a research project aiming at characterizing food products from Piedmont on the basis of chemical parameters. Differentiation between the two types of Pelaverga will likely require the determination of different chemical parameters in conjunction with volatile compounds.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 6","pages":"262-270"},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47315575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of spray drying conditions on physicochemical and functional properties of apple cider vinegar powder 喷雾干燥条件对苹果醋粉理化性能和功能性能的影响
JSFA reports Pub Date : 2023-05-18 DOI: 10.1002/jsf2.130
Ipek Altay, Peter Reimer Stubbe, Mohammad Amin Mohammadifar
{"title":"Effect of spray drying conditions on physicochemical and functional properties of apple cider vinegar powder","authors":"Ipek Altay,&nbsp;Peter Reimer Stubbe,&nbsp;Mohammad Amin Mohammadifar","doi":"10.1002/jsf2.130","DOIUrl":"10.1002/jsf2.130","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Apple cider vinegar (ACV) contains many health benefits due to its antioxidant and acetic acid content. However, the adverse effects of directly consuming the vinegar should be eliminated to make it possible to regulate its intake as a dietary supplement. The objective of the study is to optimize the spray drying operating conditions to produce ACV powder with health benefits. A central composite design (CCD) of response surface methodology (RSM) was used to optimize the spray drying process of ACV. The influences of inlet temperature (130°C–170°C), gum Arabic concentration (5%–15% w/w), and feed flow rate (1–2 kg/h) on some product and process aspects were investigated. Spray-dried powders were evaluated for their moisture content, process yield, acetic acid content, solubility, and antioxidant content.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The relationships between the model factors and final powder properties were established with ANOVA and multiple regression analysis. The model factors affected the investigated powder properties at different significance levels, where carrier concentration was the main influencing factor. Gum Arabic contributed to the retention of volatile and heat-sensitive compounds providing health benefits. All powders showed high solubility (&gt;94%) and high antioxidant recovery, and most treatments achieved a drying yield of higher than 50%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Optimization of spray drying process was achieved with application of RSM and relationships between model factors and powder properties were established. This study highlighted the feasibility of the development of ACV-based functional powder as a dietary supplement.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 6","pages":"271-281"},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jsf2.130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48286168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of cyanide intake through consumption of cassava-based diets in the Democratic Republic of Congo 在刚果民主共和国,通过食用以木薯为基础的饮食来评估氰化物摄入量
JSFA reports Pub Date : 2023-05-18 DOI: 10.1002/jsf2.131
Christus C. Miderho, Lucy G. Njue, George O. Abong'
{"title":"Assessment of cyanide intake through consumption of cassava-based diets in the Democratic Republic of Congo","authors":"Christus C. Miderho,&nbsp;Lucy G. Njue,&nbsp;George O. Abong'","doi":"10.1002/jsf2.131","DOIUrl":"10.1002/jsf2.131","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Konzo is attributed to inadequate cassava processing and consumption of cassava meals with little protein, which is characterized by a neurological disorder that causes paralysis of the lower limbs. However, the exposure to cyanide in the Mwana health zone is still unknown while the prevalence of konzo is assumed to be high compared with other areas in the Democratic Republic of Congo (DRC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The results showed that women are the most affected (56.7%), followed by young people under the age of 10 (27%), and people from low socioeconomic class at 93.4%. The prevalence of konzo in Burhinyi is 0.06%. Cyanide intake by the population of Burhinyi ranged from 26 to 271 mg/kg bw/day at the 95th percentile through consumption of cassava-based <i>ugali</i>. Margin of exposure was around 0.271 and 0.393 g/person/day.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Poor people, women, and children are more at risk for konzo disease due to a diet dominated by under processed food made from cassava. However, since 2011, the study area's konzo prevalence has remained constant.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 7","pages":"310-319"},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41559821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Approaches to check unauthorized genetically modified events in supply chain: Challenges and solutions in the Indian context 检查供应链中未经授权的转基因事件的方法:印度背景下的挑战和解决方案
JSFA reports Pub Date : 2023-04-19 DOI: 10.1002/jsf2.115
Monika Singh, Nina Papazova, Raghavendra Aminedi, Marie-Alice Fraiture, Nancy Roosens, Gurinderjit Randhawa
{"title":"Approaches to check unauthorized genetically modified events in supply chain: Challenges and solutions in the Indian context","authors":"Monika Singh,&nbsp;Nina Papazova,&nbsp;Raghavendra Aminedi,&nbsp;Marie-Alice Fraiture,&nbsp;Nancy Roosens,&nbsp;Gurinderjit Randhawa","doi":"10.1002/jsf2.115","DOIUrl":"10.1002/jsf2.115","url":null,"abstract":"<p>The approval status of a genetically modified (GM) event varies from country to country. GM events approved in one country (considered as authorized GM or AGM) may not necessarily have the same approval status in other countries (considered as unauthorized GM or UGM). Detecting UGM in the supply chain is a challenge as the genetic information is not always available. In India, four <i>Bt</i> cotton events are approved, whereas several other GM events have been imported for research purposes. Many food derivatives (non-GM) are being imported from the countries where GM events of food crops are approved so it is necessary to track the unauthorized entry of GM products. Selected consignments or food products need to be checked for GM status for regulatory compliance. In farmers' fields, the chances of unintentional introgression or adventitious presence of transgenes also need to be monitored in a systematic manner. An appropriate strategy needs to be developed to check for UGM in the food and agricultural supply chain. In this article, approaches for UGM detection have been discussed with a focus on application in the Indian context. Detection methods based on the GMO matrix, multiplex PCR, real-time PCR (qPCR), and loop-mediated isothermal amplification (LAMP) could be employed keeping in view the regulatory requirement or practical application. For checking UGM with unknown genetic construct, methods such as next-generation sequencing (NGS) may be employed. The advantages and disadvantages of the different approaches are discussed in the function of the analytical strategy and its application for control purposes.</p>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 5","pages":"184-195"},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43757779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biotive protease inhibitor from leaves of Capsicum annuum L.: Potential use as an antifungal molecule against Colletotrichum scovillei 辣椒叶片中的生物蛋白酶抑制剂:作为抗炭疽菌分子的潜在用途
JSFA reports Pub Date : 2023-04-19 DOI: 10.1002/jsf2.116
Lídia S. Pereira, Gabriel B. Taveira, Thaynã A. M. Souza, André O. Carvalho, Virginia S. Carvalho, Rosana Rodrigues, Katia Valevski S. Fernandes, Erica O. Mello, Valdirene M. Gomes
{"title":"Biotive protease inhibitor from leaves of Capsicum annuum L.: Potential use as an antifungal molecule against Colletotrichum scovillei","authors":"Lídia S. Pereira,&nbsp;Gabriel B. Taveira,&nbsp;Thaynã A. M. Souza,&nbsp;André O. Carvalho,&nbsp;Virginia S. Carvalho,&nbsp;Rosana Rodrigues,&nbsp;Katia Valevski S. Fernandes,&nbsp;Erica O. Mello,&nbsp;Valdirene M. Gomes","doi":"10.1002/jsf2.116","DOIUrl":"10.1002/jsf2.116","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>A diverse set of antimicrobial molecules have been found mainly in the seeds and fruits of <i>Capsicum annuum</i> UENF1381, accession resistant to different phytopathogenic microorganisms, like bacteria of the genus <i>Xanthomonas</i> and fungi of the genus <i>Colletotrichum</i>. However, there is little information about the defense mechanisms in other plant organs. The work aimed to study the induction of resistance in leaves and roots of <i>C</i>. <i>annuum</i> through the identification and characterization of antimicrobial peptides against <i>Colletotrichum scovillei</i>, one of the main pathogens of the crop.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Based on the results of differential protein expression in response to infection, the extracts, LC<sub>48</sub> (Control Leaf mock inoculated 48 h), and RC<sub>48</sub> (Control Root mock inoculated 48 h) were selected. Analysis on the HPLC system resulted in 23 fractions (L1 to L23) obtained from the LC<sub>48</sub> extract and 27 fractions from the RC<sub>48</sub> extract (R1 to R27). Our results show that 10 μg mL<sup>−1</sup> of the L1 fraction is able to inhibit 88.4% of fungal growth, causing cell membrane permeabilization, endogenous reactive oxygen species induction and mitochondrial activity reduction. The peptide in the L1 fraction, named <i>Ca</i>LPI, showed similarities with different sequences of proteinase inhibitors.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The study provides information on the diversity of antimicrobial proteins and peptides present in the leaves and roots of <i>C</i>. <i>annuum</i> UENF1381. With these results, we hope to contribute to the use of peptides as potential molecules in microbial control.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 5","pages":"248-257"},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43549057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chemical, biochemical and volatile profiles of saffron (Crocus sativus L.) from different growing areas of Morocco 摩洛哥不同种植区藏红花的化学、生化和挥发性特征
JSFA reports Pub Date : 2023-04-19 DOI: 10.1002/jsf2.114
Nadia Naim, Ibtissame Guirrou, Marie-Laure Fauconnier, Hanine Hafida, Abdessalem Tahiri, Ilham Madani, Rachid Lahlali, Said Ennahli
{"title":"Chemical, biochemical and volatile profiles of saffron (Crocus sativus L.) from different growing areas of Morocco","authors":"Nadia Naim,&nbsp;Ibtissame Guirrou,&nbsp;Marie-Laure Fauconnier,&nbsp;Hanine Hafida,&nbsp;Abdessalem Tahiri,&nbsp;Ilham Madani,&nbsp;Rachid Lahlali,&nbsp;Said Ennahli","doi":"10.1002/jsf2.114","DOIUrl":"10.1002/jsf2.114","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The saffron (<i>Crocus sativus</i> L.) planted area and production increased in the past decade in Morocco. This crop has been extended to new regions beyond its original main area due to the shift in climate conditions. Therefore, this study aimed to investigate the chemical characterization and the quality of saffron stigma samples collected from 11 different localities.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>According to the ISO3632 standard method, all samples were within the ISO36-32.2010 categorization range (category I and II) Contents of crocin responsible for color, picrocrocin responsible for taste, and safranal responsible for the aroma, showed significant differences between samples from different areas. The biochemical analysis revealed that samples from Boulmane (Serghina and El Mers) are rich in polyphenols (5.70 ± 0.34; 5.31 ± 0.004 mg GAE/g DW, respectively) and have an important antioxidant power (IC<sub>50</sub> for the DPPH: 231.12 ± 1.065; 236.77 ± 2109 respectively) compared to those from Taznakht and Taliouine known for their higher organoleptic quality. Interestingly, volatile profiling by gas chromatography–mass spectrometry identified the safranal, isophorone, ketoisophorone, ß-isophorone, and 1-4-cyclohexanedione, 2,2,6-trimethyl as the main volatile compounds in saffron samples from different regions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study is the first to be conducted in Morocco on saffron newly cultivated in new regions (Boulmane, Azilal, and Ain Leuh) and showed the higher potential for growing saffron, thus promoting the adoption of saffron as a substitute crop for the socio-economic development in these new areas.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 5","pages":"233-247"},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42751544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Valorization of sea buckthorn seed protein to hydrolysates: Impact on morphological, structural, functional, and antioxidant properties 沙棘籽蛋白水解物的缬氨酸化:对形态、结构、功能和抗氧化性能的影响
JSFA reports Pub Date : 2023-04-17 DOI: 10.1002/jsf2.113
Zakir Showkat Khan, Navdeep Singh Sodhi, Shemilah Fayaz, Rayees Ahmad Bakshi, Raashid Ahmad Siddiqi, Basharat N. Dar, Hari N. Mishra, Bhavnita Dhillon
{"title":"Valorization of sea buckthorn seed protein to hydrolysates: Impact on morphological, structural, functional, and antioxidant properties","authors":"Zakir Showkat Khan,&nbsp;Navdeep Singh Sodhi,&nbsp;Shemilah Fayaz,&nbsp;Rayees Ahmad Bakshi,&nbsp;Raashid Ahmad Siddiqi,&nbsp;Basharat N. Dar,&nbsp;Hari N. Mishra,&nbsp;Bhavnita Dhillon","doi":"10.1002/jsf2.113","DOIUrl":"https://doi.org/10.1002/jsf2.113","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Increasing concerns about nutritional deficiency, food security, and sustainability have emphasized work on exploring alternative and sustainable sources of protein. In this study, an initiative to extract and utilize protein concentrates, and hydrolysates from sea buckthorn seed, otherwise a waste material, is undertaken. The effect of hydrolysis using various enzymes namely pepsin (pH 2.0 and 37°C), trypsin (pH 7.0 and 37°C), and protamex (pH 7.0 and 50°C), for different time durations, namely, 30, 60, 90, and 120 min, on the structural and functional properties of sea buckthorn seed protein concentrate (SSPC), was elucidated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The emulsifying activity index and solubility of pepsin-hydrolysed SSPC increased significantly and were higher than those of trypsin and protamex hydrolysed SSPC samples. A Fourier transformation infrared study revealed that the enzyme hydrolysis treatment reduced the peak intensity in amide I, II, and III bands. A significant change was found in the β-sheet, β-turn and random coiling of the secondary structure of SSPC protein by enzymatic hydrolysis. The particle size ranged from 295.8 to 440, 388 to 713.6, 399 to 890 nm for pepsin, trypsin, and protamex, respectively. The microstructure of pepsin-treated samples showed a more porous and loose structure than native, trypsin and protamex. Antioxidant activity increased significantly for all the samples with enzyme treatments carried out for different periods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The results indicate that the protein-rich hydrolysates could create new opportunities for the development of effective techno-functional additives for use in a wide range of food formulations.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 5","pages":"222-232"},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50151551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Valorization of seabuckthorn seed protein to hydrolysates: Characterization of morphological, structural, functional, and in‐vitro radical scavenging activity 沙棘种子蛋白水解产物:形态、结构、功能和体外自由基清除活性的表征
JSFA reports Pub Date : 2023-04-17 DOI: 10.1002/jsf2.113
Z. Khan, N. S. Sodhi, Shemilah Fayaz, Rayees Ahmad Bakshi, Raashid Ahmad Siddiqi, B. N. Dar, H. Mishra, B. Dhillon
{"title":"Valorization of seabuckthorn seed protein to hydrolysates: Characterization of morphological, structural, functional, and in‐vitro radical scavenging activity","authors":"Z. Khan, N. S. Sodhi, Shemilah Fayaz, Rayees Ahmad Bakshi, Raashid Ahmad Siddiqi, B. N. Dar, H. Mishra, B. Dhillon","doi":"10.1002/jsf2.113","DOIUrl":"https://doi.org/10.1002/jsf2.113","url":null,"abstract":"","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49163064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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