JSFA reportsPub Date : 2023-07-13DOI: 10.1002/jsf2.140
Kavitha Susan Issac, Shubha Banerjee, Sahayog N. Jamdar
{"title":"Improving functional properties of rice protein isolates by hydrolysis using alcalase enzyme and assessing bioactivities of hydrolysates","authors":"Kavitha Susan Issac, Shubha Banerjee, Sahayog N. Jamdar","doi":"10.1002/jsf2.140","DOIUrl":"10.1002/jsf2.140","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Developing value-added products from rice grains is becoming increasingly significant to enhance productivity from excess rice grains. Therefore, the objective of this study was to improve the functional properties of rice protein from four rice varieties, namely, RRF-127, RRF-105, Swarna, and Protezin by enzymatic hydrolysis, and also to evaluate bioactivities like antioxidant, ACE, and DPP-IV inhibitory activities of the hydrolysates.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The alcalase hydrolysis increased protein solubility to >80% in the pH range of 4–10. The foaming capacity and stability of rice protein hydrolysates (RPHs) improved with the increase in pH. The hydrolysis significantly improved the emulsification activity index (EAI) in the pH range of 5–9. The hydrolysates showed antioxidant activity, namely, ABTS<sup>•<i>+</i></sup>radical scavenging, reducing power, and metal ion chelation. RRF-105 was found to possess the most potent antioxidant capacity among the hydrolysates. RPH could alleviate oxidative damage in erythrocytes, and their protective effects were concentration-dependent. The hydrolysates had stronger inhibition of ACE and DPP-IV enzymes. Protezin exhibited the highest ACE inhibition (63%), while Swarna demonstrated the most effective DPP-IV inhibition (66%).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Hydrolysis with alcalase improved the functional and bioactive properties of rice proteins, indicating that RPH could serve as a source of bioactive peptides or as a functional ingredient with potential properties.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 8","pages":"364-376"},"PeriodicalIF":0.0,"publicationDate":"2023-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44651826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
JSFA reportsPub Date : 2023-06-23DOI: 10.1002/jsf2.139
Heloisa Patrício Inácio, Gabriela Soster Santetti, Marina Volpato Dacoreggio, Isabel Cristina da Silva Haas, Julia Baranzelli, Tatiana Emanuelli, Rodrigo Barcellos Hoff, Aniela Pinto Kempka, Carlise Beddin Fritzen Freire, Renata Dias de Mello Castanho Amboni
{"title":"Effects of different extraction methods on the phenolic profile, antioxidant and antimicrobial activity of the coffee grounds and coffee silverskin (Coffea arabica L.)","authors":"Heloisa Patrício Inácio, Gabriela Soster Santetti, Marina Volpato Dacoreggio, Isabel Cristina da Silva Haas, Julia Baranzelli, Tatiana Emanuelli, Rodrigo Barcellos Hoff, Aniela Pinto Kempka, Carlise Beddin Fritzen Freire, Renata Dias de Mello Castanho Amboni","doi":"10.1002/jsf2.139","DOIUrl":"10.1002/jsf2.139","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>During coffee processing, a large volume of waste is generated. This study investigated the phenolic profile, antioxidant and antibacterial activity of coffee grounds and silverskin using different extraction methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), and ultrasound-assisted enzyme extraction (UAEE) were effective for extraction of polyphenols, with UAE being the method that promoted the highest extraction of polyphenols. The coffee ground and coffee silverskin extracts showed high antioxidant capacity for the three methods ABTS, DPPH, and ORAC (4013.36–3241.89 μmol Trolox/g; 1.82–2.06 IC<sub>50</sub> μg/mL; and 26.60–16.20 μmol Trolox/mL, respectively). The phenolic profile demonstrated that most of the compounds in both matrices were chlorogenic acid, caffeic acid, 2,4-DHBA, 2,5-DHBA, and 3,4-DHB. EAE promoted a greater release of polyphenols in the coffee ground (2676.42 μg/g), while UAEE was more effective for the coffee silverskin (2111.54 μg/g). The Principal Component Analysis grouped the samples according to the extraction method, based on their content and phenolic potential, for coffee ground and coffee silverskin. As for antibacterial activity, the most expressive result was for Gram-positive bacteria. For both matrices, when extracted by the UAEE method, they presented the minimum inhibitory concentration to inhibit the growth of <i>Bacillus subtilis</i>, <i>Bacillus cereus</i>, and <i>Listeria monocytogenes</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The extraction methods used are environmentally safe alternatives, enabling the reuse of natural compounds and contributing to the circular economy, seeking to transform waste into raw material for the extraction of compounds of interest and possible subsequent application in agriculture as biofertilizers or bioherbicides.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 8","pages":"354-363"},"PeriodicalIF":0.0,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45394402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Target-mediated suppression of rolling circle amplification for label-free detection of aflatoxin B1","authors":"Lili Yao, Yuqin Cheng, Baolei Dong, Ling Yan, Hao Qu, Yu Mao, Changhong Liu, Lei Zheng","doi":"10.1002/jsf2.138","DOIUrl":"10.1002/jsf2.138","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) has been considered as a prevailing natural carcinogenic mycotoxin in various food and feedstuff products, posing serious endangers to human health. The quantitative and selective detection of AFB<sub>1</sub> was significantly essential for food safety control.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We developed a label-free fluorescent aptasensor using a target-binding aptamer probe as the rolling circle amplification (RCA) primer for AFB<sub>1</sub> detection. In the presence of AFB<sub>1</sub>, the probe preferentially combined with AFB<sub>1</sub> rather than binding to the circular template, inhibiting the initiation of RCA process. The detection limit of the proposed method was as low as 6.6 nM, and the linear dynamic response range was four orders of magnitude wide. Besides, it revealed excellent specificity for AFB<sub>1</sub> and high-quality detection performance in food samples.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The presented detection method was simple, rapid, and economical, which indicated a promising application in food safety monitoring.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 8","pages":"346-353"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47441120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Traditional Asian plant-based fermented foods as vitamin B12 sources: A mini-review","authors":"Kyohei Koseki, Fei Teng, Tomohiro Bito, Fumio Watanabe","doi":"10.1002/jsf2.137","DOIUrl":"10.1002/jsf2.137","url":null,"abstract":"<p>Plant-based foods, such as vegetables and beans, are deficient in vitamin B<sub>12</sub> (B<sub>12</sub>). If plant-based foods containing adequate B<sub>12</sub> can be produced microbially, incorporating them into a vegetarian diet would prevent B<sub>12</sub> deficiency. Asian indigenous fermented foods from soybeans, vegetables, and tea leaves, are commercially accessible. The B<sub>12</sub> level in the fermented products has been characterized to determine whether they may serve as a viable source of B<sub>12</sub> in humans. Since their B<sub>12</sub> content is very low (<0.5 μg/100 g wet weight), most traditional Asian plant-based fermented foods do not appear to serve as adequate daily B<sub>12</sub> sources. However, some stinky tofu and pickled vegetable products contain >10 μg of B<sub>12</sub> per 100 g wet weight. Nonetheless, further clinical research is warranted to clarify whether these products can increase the serum B<sub>12</sub> levels of humans.</p>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 7","pages":"294-298"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41390128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
JSFA reportsPub Date : 2023-06-15DOI: 10.1002/jsf2.136
Jing Mo, Wenbin Wang, Yongyi Wang, Huachun Cheng, Xiaofang Li, Bo Wang
{"title":"Rapid and simultaneous identification of Lonicera Japonicae Flos and Lonicerae Flos using MLPA-HRM and rhPCR-HRM","authors":"Jing Mo, Wenbin Wang, Yongyi Wang, Huachun Cheng, Xiaofang Li, Bo Wang","doi":"10.1002/jsf2.136","DOIUrl":"10.1002/jsf2.136","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Lonicera Japonicae Flos (LJF) and Lonicerae Flos (LF), derived from different species of <i>Lonicera</i>, are two different types of traditional Chinese medicine (TCM) according to their differences in composition and effectiveness. Because of the high price, good effectiveness of the medicinal, and functional values, LJF is often adulterated by LF in the market. Therefore, two single nucleotide polymorphism-based genotyping methods, multiplex ligation-dependent probe amplification high-resolution melting (MLPA-HRM) and RNase H2-dependant PCR (rhPCR)-HRM, were established for the identification of LF and LJF.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Highly specific MLPA probes and rhPCR-primers were designed based on the <i>trnL-trnF</i> regions. The melting temperature (<i>T</i><sub><i>m</i></sub>) of amplicons was derived from the peak of the HRM curve, and the differences in <i>T</i><sub><i>m</i></sub> values were used to identify LJF and LF. The two methods have both shown good specificity for identifying LJF and LF without cross-reaction, and have high sensitivity with the detection limits of 0.1 ng DNA template. For mixed samples, MLPA-HRM could detect LJF mixed with 10% LF while rhPCR-HRM could detect 5% LF in mixed samples. The MLPA-HRM and rhPCR-HRM were performed on DNA extracted from 54 samples of LJF and LF randomly selected from the medicinal material market, all samples were successfully identified and further verified with DNA barcoding.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>MLPA-HRM and rhPCR-HRM methods developed in our study not only provided fast, simple, sensitive, and practical identification of LJF and LF, but also provided technical references for the adulteration identification of other herb-medicines, they are of great significance to the quality control and safe use of plant medicinal materials.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 7","pages":"331-341"},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45554148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
JSFA reportsPub Date : 2023-05-25DOI: 10.1002/jsf2.134
J. Alejandro Arboleda-Murillo, Andres F. Cañon-Ibarra, Leidy T. Sanchez, Magda I. Pinzon, Cristian C. Villa
{"title":"Applications of curcumin loaded nanoemulsions in photoinactivation of Aspergillus niger spores","authors":"J. Alejandro Arboleda-Murillo, Andres F. Cañon-Ibarra, Leidy T. Sanchez, Magda I. Pinzon, Cristian C. Villa","doi":"10.1002/jsf2.134","DOIUrl":"https://doi.org/10.1002/jsf2.134","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p><i>Aspergillus niger</i> is one of the most common fungal contaminants of food products, leading to several economic losses on fruit, vegetables and other food products during storage. Over the years, several new techniques have been developed to control fungal growth, including photodynamic inactivation, in which excitation of a photosensitizer in the right wavelength leads to ROS production and microbial death.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>On this paper, curcumin was used as a natural photosentitizer against <i>A. niger</i> spores. Nanoemulsions were used in order to encapsulated curcumin and increase its water solubility. Results showed that spores inhibition could reach up to 80% after 3 min of irradiation while values for un-encapsulated curcumin were around 30%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Results presented show that nanoemulsions could be use to enhance the photoinactivation properties of curcumin, against fungal spores from <i>A. niger.</i> This type of process can be use for food decontamination and preservation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 7","pages":"299-303"},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50143886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
JSFA reportsPub Date : 2023-05-25DOI: 10.1002/jsf2.135
S. Veluri, O. Olukosi
{"title":"Influence of adaptation length on metabolizable energy assay of maize and barley supplemented with or without glucanase determined by index method in broilers","authors":"S. Veluri, O. Olukosi","doi":"10.1002/jsf2.135","DOIUrl":"https://doi.org/10.1002/jsf2.135","url":null,"abstract":"","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42813396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
JSFA reportsPub Date : 2023-05-25DOI: 10.1002/jsf2.134
J. A. Arboleda‐Murillo, Andrés F. Cañon-Ibarra, Leidy T. Sanchez, M. I. Pinzón, Cristian C. Villa
{"title":"Applications of Curcumin Loaded Nanoemulsions in Photoinactivation of\u0000 A. Niger\u0000 spores","authors":"J. A. Arboleda‐Murillo, Andrés F. Cañon-Ibarra, Leidy T. Sanchez, M. I. Pinzón, Cristian C. Villa","doi":"10.1002/jsf2.134","DOIUrl":"https://doi.org/10.1002/jsf2.134","url":null,"abstract":"","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47671390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}