{"title":"目标介导的滚动圈扩增抑制黄曲霉毒素B1无标记检测","authors":"Lili Yao, Yuqin Cheng, Baolei Dong, Ling Yan, Hao Qu, Yu Mao, Changhong Liu, Lei Zheng","doi":"10.1002/jsf2.138","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) has been considered as a prevailing natural carcinogenic mycotoxin in various food and feedstuff products, posing serious endangers to human health. The quantitative and selective detection of AFB<sub>1</sub> was significantly essential for food safety control.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>We developed a label-free fluorescent aptasensor using a target-binding aptamer probe as the rolling circle amplification (RCA) primer for AFB<sub>1</sub> detection. In the presence of AFB<sub>1</sub>, the probe preferentially combined with AFB<sub>1</sub> rather than binding to the circular template, inhibiting the initiation of RCA process. The detection limit of the proposed method was as low as 6.6 nM, and the linear dynamic response range was four orders of magnitude wide. Besides, it revealed excellent specificity for AFB<sub>1</sub> and high-quality detection performance in food samples.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The presented detection method was simple, rapid, and economical, which indicated a promising application in food safety monitoring.</p>\n </section>\n </div>","PeriodicalId":93795,"journal":{"name":"JSFA reports","volume":"3 8","pages":"346-353"},"PeriodicalIF":0.0000,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Target-mediated suppression of rolling circle amplification for label-free detection of aflatoxin B1\",\"authors\":\"Lili Yao, Yuqin Cheng, Baolei Dong, Ling Yan, Hao Qu, Yu Mao, Changhong Liu, Lei Zheng\",\"doi\":\"10.1002/jsf2.138\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) has been considered as a prevailing natural carcinogenic mycotoxin in various food and feedstuff products, posing serious endangers to human health. The quantitative and selective detection of AFB<sub>1</sub> was significantly essential for food safety control.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>We developed a label-free fluorescent aptasensor using a target-binding aptamer probe as the rolling circle amplification (RCA) primer for AFB<sub>1</sub> detection. In the presence of AFB<sub>1</sub>, the probe preferentially combined with AFB<sub>1</sub> rather than binding to the circular template, inhibiting the initiation of RCA process. The detection limit of the proposed method was as low as 6.6 nM, and the linear dynamic response range was four orders of magnitude wide. Besides, it revealed excellent specificity for AFB<sub>1</sub> and high-quality detection performance in food samples.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>The presented detection method was simple, rapid, and economical, which indicated a promising application in food safety monitoring.</p>\\n </section>\\n </div>\",\"PeriodicalId\":93795,\"journal\":{\"name\":\"JSFA reports\",\"volume\":\"3 8\",\"pages\":\"346-353\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-06-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"JSFA reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jsf2.138\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"JSFA reports","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jsf2.138","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Target-mediated suppression of rolling circle amplification for label-free detection of aflatoxin B1
Background
Aflatoxin B1 (AFB1) has been considered as a prevailing natural carcinogenic mycotoxin in various food and feedstuff products, posing serious endangers to human health. The quantitative and selective detection of AFB1 was significantly essential for food safety control.
Results
We developed a label-free fluorescent aptasensor using a target-binding aptamer probe as the rolling circle amplification (RCA) primer for AFB1 detection. In the presence of AFB1, the probe preferentially combined with AFB1 rather than binding to the circular template, inhibiting the initiation of RCA process. The detection limit of the proposed method was as low as 6.6 nM, and the linear dynamic response range was four orders of magnitude wide. Besides, it revealed excellent specificity for AFB1 and high-quality detection performance in food samples.
Conclusion
The presented detection method was simple, rapid, and economical, which indicated a promising application in food safety monitoring.
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