Analytical science advances最新文献

{"title":"Challenges and recent advances in quantitative mass spectrometry-based metabolomics","authors":"Nathan Ghafari,&nbsp;Lekha Sleno","doi":"10.1002/ansa.202400007","DOIUrl":"10.1002/ansa.202400007","url":null,"abstract":"<p>The field of metabolomics has gained tremendous interest in recent years. Whether the goal is to discover biomarkers related to certain pathologies or to better understand the impact of a drug or contaminant, numerous studies have demonstrated how crucial it is to understand variations in metabolism. Detailed knowledge of metabolic variabilities can lead to more effective treatments, as well as faster or less invasive diagnostics. Exploratory approaches are often employed in metabolomics, using relative quantitation to look at perturbations between groups of samples. Most metabolomics studies have been based on metabolite profiling using relative quantitation, with very few studies using an approach for absolute quantitation. Using accurate quantitation facilitates the comparison between different studies, as well as enabling longitudinal studies. In this review, we discuss the most widely used techniques for quantitative metabolomics using mass spectrometry (MS). Various aspects will be addressed, such as the use of external and/or internal standards, derivatization techniques, in vivo isotopic labelling, or quantitative MS imaging. The principles, as well as the associated limitations and challenges, will be described for each approach.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 5-6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141473392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted and untargeted metabolomics and lipidomics in dried blood microsampling: Recent applications and perspectives","authors":"Pauline Couacault,&nbsp;Dennisse Avella,&nbsp;Sara Londoño-Osorio,&nbsp;Ana S. Lorenzo,&nbsp;Ana Gradillas,&nbsp;Olli Kärkkäinen,&nbsp;Elizabeth Want,&nbsp;Michael Witting","doi":"10.1002/ansa.202400002","DOIUrl":"10.1002/ansa.202400002","url":null,"abstract":"<p>Blood microsampling (BµS) offers an alternative to conventional methods that use plasma or serum for profiling human health, being minimally invasive and cost effective, especially beneficial for vulnerable populations. We present a non-systematic review that offers a synopsis of the analytical methods, applications and perspectives related to dry blood microsampling in targeted and untargeted metabolomics and lipidomics research in the years 2022 and 2023. BµS shows potential in neonatal and paediatric studies, therapeutic drug monitoring, metabolite screening, biomarker research, sports supervision, clinical disorders studies and forensic toxicology. Notably, dried blood spots and volumetric absorptive microsampling options have been more extensively studied than other volumetric technologies. Therefore, we suggest that a further investigation and application of the volumetric technologies will contribute to the use of BµS as an alternative to conventional methods. Conversely, we support the idea that harmonisation of the analytical methods when using BµS would have a positive impact on its implementation.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 5-6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202400002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141344304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection and association of 12.1 kb deletion within the high mobility AT-hook 2 gene in the Netherlands dwarf rabbit (Oryctolagus Cuniculus)","authors":"Tai Duc Nguyen,&nbsp;Lam Van Dang,&nbsp;Phuong Nhu Nguyen Tran,&nbsp;Dai Van Nguyen,&nbsp;Anh Phu Nam Bui","doi":"10.1002/ansa.202300050","DOIUrl":"10.1002/ansa.202300050","url":null,"abstract":"<p>Rabbits are mainly bred for human consumption and medical research. However, it has been recently showed that several rabbit breeds are also kept as pets for human leisure. The Netherlands dwarf rabbit is currently in the immense interest of many Vietnamese customers due to its personality and miniature stature. However, 12.1 kb deletion from position 44,709,089 to 44,721,236 bp in the high mobility AT-hook 2 (HMGA2) gene on chromosome 4 was identified as the structural variant causing dwarfism and altered craniofacial development in this breed. It has been documented that HMGA2 plays an important role in regulating growth and individuals with genotype HMGA2 <i>del/del</i> are fatal several days after birth. Despite the economically high value of the Netherlands dwarf rabbit, there has been no study on the genetic survey of lethal alleles in this breed in Vietnam. The aim of this study is to develop a fast and reliable method to screen the frequency of lethal alleles of HMGA2 in the South of Vietnam. Rabbit saliva was collected, and DNA extraction was followed. Multiplex polymerase chain reaction (PCR) with three primers was optimized and performed to detect the presence of 12.1 kb deletion within the HMGA2 sequence. Our data showed that the 12.1 kb deletion in the Netherlands dwarf rabbit population was detected by our optimized multiplex PCR. In 100 rabbit animals, 34 and 16 individuals were homozygous wild type (<i>+/+</i>) and homozygous mutant (del/del), respectively, while 50 rabbits were heterozygous. The frequency of HMGA2 lethal allele carrier was 66% (66/100 individuals). Our results indicated that we successfully developed a fast, accurate multiplex PCR to detect carrier individuals. Verification of the genotypes was followed by sequencing. We recommend implementing our multiplex PCR procedure in genetic selection for carrier and homozygous wild-type animals in the mating scheme to prevent the lethality of the rabbit offspring. Additionally, awareness should be raised among rabbit breeders to monitor the genetic makeup of the Netherlands dwarf rabbit populations. However, due to the limitation of the sample size, more samples should be taken in future studies to obtain the genetic frequency of the HMGA2 lethal allele more accurately.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 7-8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202300050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141273279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ensuring food safety: Microfluidic-based approaches for the detection of food contaminants","authors":"Tom Kasputis,&nbsp;Kait Elizabeth Hosmer,&nbsp;Yawen He,&nbsp;Juhong Chen","doi":"10.1002/ansa.202400003","DOIUrl":"10.1002/ansa.202400003","url":null,"abstract":"<p>Detecting foodborne contamination is a critical challenge in ensuring food safety and preventing human suffering and economic losses. Contaminated food, comprising biological agents (e.g. bacteria, viruses and fungi) and chemicals (e.g. toxins, allergens, antibiotics and heavy metals), poses significant risks to public health. Microfluidic technology has emerged as a transformative solution, revolutionizing the detection of contaminants with precise and efficient methodologies. By manipulating minute volumes of fluid on miniaturized systems, microfluidics enables the creation of portable chips for biosensing applications. Advancements from early glass and silicon devices to modern polymers and cellulose-based chips have significantly enhanced microfluidic technology, offering adaptability, flexibility, cost-effectiveness and biocompatibility. Microfluidic systems integrate seamlessly with various biosensing reactions, facilitating nucleic acid amplification, target analyte recognition and accurate signal readouts. As research progresses, microfluidic technology is poised to play a pivotal role in addressing evolving challenges in the detection of foodborne contaminants. In this short review, we delve into various manufacturing materials for state-of-the-art microfluidic devices, including inorganics, elastomers, thermoplastics and paper. Additionally, we examine several applications where microfluidic technology offers unique advantages in the detection of food contaminants, including bacteria, viruses, fungi, allergens and more. This review underscores the significant advancement of microfluidic technology and its pivotal role in advancing the detection and mitigation of foodborne contaminants.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 5-6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202400003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140705195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biopolymer-based intelligent packaging integrated with natural colourimetric sensors for food safety and sustainability","authors":"Ivy Chiu,&nbsp;Tianxi Yang","doi":"10.1002/ansa.202300065","DOIUrl":"10.1002/ansa.202300065","url":null,"abstract":"<p>Increasing concerns about global food safety and security demands innovative solutions, particularly in food packaging technologies. This review paper investigates the advanced integration of natural colourimetric sensors with biopolymer-based packaging materials, with a focus on developments over the past 5 years. These sensors change colour in response to environmental stimuli such as oxygen, temperature, pH and relative humidity, intuitively indicating food freshness and safety. The paper emphasizes the recent advancements in using natural colourants, such as alizarin, anthocyanins, betacyanins, chlorophyll, curcumin and shikonin. When combined with either natural or synthetic biopolymers, these colourants contribute to a sustainable and eco-friendly approach to food packaging. Such technological advances could notably decrease the incidence of foodborne illnesses by signaling potential spoilage or contamination, while also addressing food wastage by providing clear indications of edibility. Although challenges remain in sensor longevity and widespread adoption, the prospects for biopolymer-based food packaging with embedded natural colourimetric sensors are promising.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 5-6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202300065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140733640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiating the aromatic positional isomers of methylbuphedrones and methoxybuphedrones via chemical ionization-mass spectrometry","authors":"Shinji Tsunoi,&nbsp;Tomohiro Yasuhisa,&nbsp;Takahiro Hisasue,&nbsp;Itaru Suzuki,&nbsp;Ikuya Shibata","doi":"10.1002/ansa.202300064","DOIUrl":"10.1002/ansa.202300064","url":null,"abstract":"<p>Discrimination of aromatic positional isomers of methylbuphedrones and methoxybuphedrones was successfully achieved. Meta isomers were discriminated by chemical ionization-tandem mass spectrometry (CI-MS/MS) using acetonitrile as a reagent gas. Furthermore, all the aromatic positional isomers were discriminated by CI-MS/MS using vinyltrimethylsilane as a reagent gas.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 3-4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202300064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139786836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical, chemical and biological characteristics of clays from Durban (South Africa) for applications in cosmetics","authors":"S'busiso M Nkosi,&nbsp;Nokukhanya Thembane","doi":"10.1002/ansa.202300062","DOIUrl":"10.1002/ansa.202300062","url":null,"abstract":"<p>Clay soils are rock-decomposed materials comprised of both clay- and non-clay-like minerals. Clays' physiochemical and mineralogical composition determines their applicability use in cosmetics. Because of their high bioburden, they must be effectively characterized before being incorporated into cosmetics. The scope of the current study was to characterize two different samples of red and white clays for their physical, chemical and biological properties; mined from Durban, South Africa. Characterization was performed using techniques like X-ray fluorescence, X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscope, hydrogen potential, soil colour, oil absorption, swelling capacity, texture, bulk density, plastic and apparent viscosity, sun protection factor and microbiological analysis.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 3-4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202300062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139851386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two common pitfalls in the analysis of water-stable isotopologues with cryogenic vacuum extraction and cavity ring-down spectroscopy","authors":"Simon Haberstroh,&nbsp;Angelika Kübert,&nbsp;Christiane Werner","doi":"10.1002/ansa.202300053","DOIUrl":"10.1002/ansa.202300053","url":null,"abstract":"<p>Water stable isotopologue analysis is widely used to disentangle ecohydrological processes. Yet, there are increasing reports of measurement uncertainties for established and emerging methods, such as cryogenic vacuum extraction (CVE) or cavity ring-down spectroscopy (CRDS). With this study, we investigate two pitfalls, that potentially contribute to uncertainties in water-stable isotopologue research. To investigate fractionation sources in CVE, we extracted pure water of known isotopic composition with cotton, glass wool or without cover and compared the isotopologue results with non-extracted reference samples. To characterise the dependency of δ²H and δ¹⁸O on the water mixing ratio in CRDS, which is of high importance for in-situ applications with large natural variations in mixing ratios, we chose samples with a large range of isotopic compositions and determined δ²H and δ¹⁸O for different water mixing ratios with two CRDS analysers (Picarro, Inc.). Cotton wool had a strong fractionation effect on δ²H values, which increased with more ²H-enriched samples. δ²H and δ¹⁸O values showed a strong dependency on the water mixing ratio analysed with CRDS with differences of up to 34.5‰ (δ²H) and 3.9‰ (δ¹⁸O) for the same sample at different mixing ratios. CVE and CRDS, now routinely applied in water stable isotopologue research, come with pitfalls, namely fractionation effects of cover materials and water mixing ratio dependencies of δ²H and δ¹⁸O, which can lead to erroneous isotopologue results and thus, invalid conclusions about (ecohydrological) processes. These practical issues identified here should be reported and addressed adequately in water-stable isotopologue research.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 3-4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202300053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139598355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening stabilisers for cyanoenone triterpenoid TX101 in rat plasma samples by simultaneous analysis of parent drug and the epoxidation product","authors":"Lynn Tian,&nbsp;Qingguo Tian,&nbsp;Edward Tamer","doi":"10.1002/ansa.202300058","DOIUrl":"10.1002/ansa.202300058","url":null,"abstract":"<p>In the development of bioanalytical methods, stabilizing drug molecules in biological matrices is crucial for ensuring reliable exposure data in pharmacokinetic and toxicokinetic sample analyses. This study focuses on the evaluation of stabilizing effects on the synthetic triterpenoid TX101, a cyanoenone triterpenoid Nrf2 activator with known instability in plasma samples. The molecule's unsaturated double bond is susceptible to oxidation, either nonenzymatically via oxygen or enzymatically through cytochrome P450 enzyme-catalyzed epoxidation. The research explores the impact of antioxidants (L-ascorbic acid, sodium metabisulfite, sodium sulfite) and P450 enzyme inhibitors (sodium diethyldithiocarbamate, memantine hydrochloride, 1-aminobenzotriazole) on TX101 stability in rat plasma samples. Results reveal that adding 2.5 mg/mL sodium sulfite or sodium metabisulfite effectively inhibits the nonenzymatic oxidation of TX101 to TX101-epoxide, while L-ascorbic acid shows minimal stabilizing effect. Among P450 enzyme inhibitors, sodium diethyldithiocarbamate and memantine hydrochloride exhibit modest stabilizing effects, likely attributed to their antioxidant activity. The developed High-formance liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) method, incorporating Supported Liquid Extraction for sample cleanup, allows simultaneous monitoring of TX101 and TX101-epoxide. Application of this method in a rat dose-range finding study confirms successful inhibition of TX101-epoxide formation in samples treated with sodium sulfite or sodium metabisulfite. Overall, the study emphasizes the importance of stabilizers in preventing nonenzymatic oxidation reactions during sample storage, providing valuable insights for bioanalytical method development and validation.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 1-2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202300058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139524089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In situ simultaneous measuring method for the determination of key processes of soil organic carbon cycling: Soil microbial respiration using laser spectrometry","authors":"Hongzhao Yuan,&nbsp;Zhen He,&nbsp;Liping Zhang,&nbsp;Jiurong Wang,&nbsp;Zhenke Zhu,&nbsp;Tida Ge","doi":"10.1002/ansa.202300054","DOIUrl":"https://doi.org/10.1002/ansa.202300054","url":null,"abstract":"<p>Rationale: Soil microbial heterotrophic C-CO₂ respiration is important for C cycling. Soil CO₂ differentiation and quantification are vital for understanding soil C cycling and CO₂ emission mitigation. Presently, soil microbial respiration (SR) quantification models are based on native soil organic matter (SOM) and require consistent monitoring of δ¹³C and CO₂.</p><p>Methods: We present a new apparatus for achieving in situ soil static chamber incubation and simultaneous CO₂ and δ¹³C monitoring by cavity ring-down spectroscopy (CRDS) coupled with a soil culture and gas introduction module (SCGIM) with multi-channel. After a meticulous five-point inter-calibration, the repeatability of CO₂ and δ¹³C values by using CRDS-SCGIM were determined, and compared with those obtained using gas chromatography (GC) and isotope ratio mass spectrometry (IRMS), respectively. We examined the method regarding quantifying SR with various concentrations and enrichment of glucose and then applied it to investigate the responses of SR to the addition of different exogenous organic materials (glucose and rice residues) into paddy soils during a 21-day incubation.</p><p>Results: The CRDS-SCGIM CO₂ and δ¹³C measurements were conducted with high precision (&lt; 1.0 µmol/mol and 1‰, respectively). The optimal sampling interval and the amount added were not exceeded 4 h and 200 mg C/100 g dry soil in a 1 L incubation bottle, respectively; the ¹³C-enrichment of 3%–7% was appropriate. The total SR rates observed were 0.6–4.2 µL/h/g and the exogenous organic materials induced -49%–28% of priming effects in native SOM mineralisation.</p><p>Conclusions: Our results show that CRDS-SCGIM is a method suitable for the quantification of soil microbial CO₂ respiration, requiring less extensive lab resources than GC/IRMS.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 1-2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202300054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139716978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}