Analytical science advances最新文献

{"title":"Emerging Scientists in Analytical Sciences: Zhuoheng Zhou","authors":"Zhuoheng Zhou","doi":"10.1002/ansa.202400057","DOIUrl":"https://doi.org/10.1002/ansa.202400057","url":null,"abstract":"<p>Through a collection of editorials titled “Emerging Scientists in Analytical Sciences,” we aim to spotlight promising individuals who are actively engaged in the realm of analytical sciences. For this editorial, we invited Zhuoheng Zhou who recently submitted his PhD thesis at the Vrije Universiteit Brussel.</p><p>During my junior high school years, I was very grateful to have attended a scientific event named “<i>knowing about colours</i>”, where teachers demonstrated the light dispersion through a prism and the separation of chlorophyll green and carotenoid yellow by a piece of filter paper (until many years later that I realized it was the “<i>Eureka moment</i>” of chromatography). I was fascinated by the hidden complexity of mother nature, as much as the fact that it tends to leave us a backdoor to have a glimpse of the core. There was no such thing as attractive to a teenager as solving riddles with obscure hints scattered here and there. I later read about the biography of Antoine Lavoisier and his illumination on the essence of combustion [<span>1</span>]. The profound impact of his quantitative and analytical thinking on modern chemistry research made me realize that analytical science is <i>de facto</i> pro-science, the philosophies, methodologies and techniques it comprises are primers for any scientific research activities. To understand something, you have to “see” or “feel” it first.</p><p>I also owe my special thanks to Professor Bo Zhang at Xiamen University for hosting me as a research trainee during the summer of my second bachelor's year. This was the first time I had a chance to step on the floor of a research lab and to have a flavour of modern analytical instruments (mainly chromatographs, electrophoresis apparatus and spectrometers). Despite my “greenness”, I was still assigned to a small project on developing a fast reverse-phase chromatographic method as the second-separation dimension in a two-dimensional liquid chromatography (2D-LC) set-up, following a first-dimension strong cation-exchange separation, for intact protein profiling in velvet antler (a constituent of traditional Chinese medicines). The trainee program had a very generous deadline which permitted me to start with extensive exploratory scouting runs (a nicer way to say “trial-and-error”) and to get myself familiarized with various column chemistries and separation conditions (although in a rather primitive and intuitive way). I remember vividly the goose bumps that I had when I saw my first chromatogram on the screen with five distinctive peaks, only five minutes after I manually injected some sort of colourless, transparent, and homogeneous liquid that a senior prepared for me. The joy of knowing the supposedly unknown (more like finishing the last chapter of a detective fiction book) sparked my deep interest in the world of analytical science and particularly separation science up to this day.</p><p>My PhD thesis comprised of two major parts. The first","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 9-10","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202400057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142525687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive and Cost-Effective Tools in the Detection of Ovarian Cancer Biomarkers","authors":"Anis Elhami,&nbsp;Ahmad Mobed,&nbsp;Reza Soleimany,&nbsp;Yalda Yazdani,&nbsp;Esmat Sadat Kazemi,&nbsp;Mahya Mohammadi,&nbsp;Hossein Saffarfar","doi":"10.1002/ansa.202400029","DOIUrl":"https://doi.org/10.1002/ansa.202400029","url":null,"abstract":"<p>Women diagnosed with late-stage ovarian cancer suffer a very high rate of mortality. Accordingly, it is imperative to detect and diagnose the disease as early as possible in its development. Achievement of this aim implies relatively large-scale screening of women at an age of clinical significance through assay of biomarkers for disease present in blood or serum. Biosensor detection offers an attractive technology for the automated detection of such species. Among several biomarkers that have been identified that are present in patients with ovarian cancer, the only one that is commonly tested for in clinical use is cancer antigen 125, which is considered to be a poor biomarker for the disease. Here, we describe several biosensors that developed in the past decade for the detection of ovarian cancer biomarkers such as CA125, human epididymis protein 4 (HE4) and apolipoprotein A1. The challenges presented by the fabrication of biosensor devices for detecting ovarian cancer and the limited number of biosensors developed for this purpose are discussed.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 9-10","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202400029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142525248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preprocessing of spectroscopic data to highlight spectral features of materials","authors":"Francisco Javier Esquivel,&nbsp;José Luis Romero-Béjar,&nbsp;José Antonio Esquivel","doi":"10.1002/ansa.202400018","DOIUrl":"https://doi.org/10.1002/ansa.202400018","url":null,"abstract":"<p>The study of the extensive data sets generated by spectrometers, which are of the type commonly referred to as big data, plays a crucial role in extracting valuable information on mineral composition in various fields, such as chemistry, geology, archaeology, pharmacy and anthropology. The analysis of these spectroscopic data falls into the category of big data, which requires the application of advanced statistical methods such as principal component analysis and cluster analysis. However, the large amount of data (big data) recorded by spectrometers makes it very difficult to obtain reliable results from raw data. The usual method is to carry out different mathematical transformations of the raw data. Here, we propose to use the affine transformation for highlight the underlying features for each sample. Finally, an application to spectroscopic data collected from minerals or rocks recorded by NASA's Jet Propulsion Laboratory is performed. An illustrative example has been included by analysing three mineral samples, which have different diageneses and parageneses and belong to different mineralogical groups.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 9-10","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202400018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Potential of the Sulfated Exopolysaccharides From the Brown Microalga Halamphora sp.: Antioxidant, Antimicrobial, and Antiapoptotic Profiles","authors":"Fatma Ben Mansour,&nbsp;Wassim Guermazi,&nbsp;Mohamed Chamkha,&nbsp;Khaled Bellassoued,&nbsp;Hichem Ben Salah,&nbsp;Abdel Halim Harrath,&nbsp;Waleed Aldahmash,&nbsp;Md Ataur Rahman,&nbsp;Habib Ayadi","doi":"10.1002/ansa.202400030","DOIUrl":"https://doi.org/10.1002/ansa.202400030","url":null,"abstract":"<p>This study aims to investigate the physicochemical characteristics of the exopolysaccharides (EPS) extracted from the microalgae species <i>Halamphora</i> sp., as well as to evaluate their antioxidant, antibacterial, and anti-apoptotic activities. The crude extracellular polysaccharides from the halophilic diatom <i>Halamphora</i> sp. were found to be extracellular heterosulfated anionic polysaccharides containing carbohydrates (76.33 ± 1.80%), proteins (0.15 ± 0.02%), uronic acids (5.44 ± 0.08%) and sulfate (7.56 ± 0.86%). The lowest protein (0.24%) and lipid (0.15%) contents suggested that EPS was highly pure. Gas chromatography–mass spectrometry analysis revealed that the carbohydrate fraction consisted of xylose, <span>l</span>-galactose, <span>d</span>-galactose, glucose, ribitol, mannose, and inositol with corresponding mole percentages of 40.55, 13.25, 13.00, 9.95, 9.82, 2.90, and 2.28, respectively. In vitro, tests showed a high total antioxidant capacity probably related to <span>l</span>-galactose followed by <span>d</span>-galactose, uronic acid, and ribitol. In addition, extracellular polysaccharides (EPS) demonstrated effective antimicrobial Gram + properties with inhibition zones ranging from 10 to 12 mm. Molecular docking showed an antiapoptotic effect, as the best docking score was generated due to the interaction of xylose and caspase 3 (−6.9 kcal/mol) and <span>l</span>-galactose and caspase 3 (−5 kcal/mol). Overall, the findings of this study suggest the possibility of using the EPS extract of <i>Halamphora</i> sp. as an additive for nutraceutical and cosmetic purposes.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 9-10","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202400030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142525570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of orange fruit peel extract concentration on the synthesis of zinc oxide nanoparticles","authors":"Emebet Wondmnew,&nbsp;Getachew Tizazu","doi":"10.1002/ansa.202400023","DOIUrl":"https://doi.org/10.1002/ansa.202400023","url":null,"abstract":"<p>In this investigation, the impact of reducing agent concentration on the synthesis of zinc oxide nanoparticles (ZnO NPs) was examined. During the synthesis, an assessment of ionic conductivity was carried out, revealing a significant increase in conductivity prior to the introduction of the reducing agent, followed by a sharp decrease upon its addition. Characterization of the ZnO NPs involved ultraviolet-visible spectroscopy, scanning electron microscopy, Fourier-transform infrared spectroscopy, and, X-ray diffraction analysis. The outcomes suggest that the characteristics of the ZnO NPs are influenced by the concentration of the reducing agent during the synthesis process. Notably, the ZnO NPs synthesized with a higher concentration of reducing agent exhibited a narrower optical band gap and increased surface energy. Furthermore, employing a concentration of 0.5 v/v resulted in the rapid production of NPs with relatively uniform sizes. Conversely, concentrations below 0.5 v/v lead to slow formation, while concentrations exceeding 0.5 v/v yielded non-uniform NPs.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 7-8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202400023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Future worldwide coronavirus disease 2019 epidemic predictions by Gaidai multivariate risk evaluation method","authors":"Oleg Gaidai,&nbsp;Yu Cao,&nbsp;Yan Zhu,&nbsp;Alia Ashraf,&nbsp;Zirui Liu,&nbsp;Hongchen Li","doi":"10.1002/ansa.202400027","DOIUrl":"https://doi.org/10.1002/ansa.202400027","url":null,"abstract":"<p>Accurate estimation of pandemic likelihood in every US state of interest and at any time. Coronavirus disease 2019 (COVID-19) is an infectious illness with a high potential for global dissemination and low rates of fatality and morbidity, placing some strains on national public health systems. This research intends to benchmark a novel technique, that enables hazard assessment, based on available clinical data, and dynamically observed patient numbers while taking into account pertinent territorial and temporal mapping. Multicentre, population-based, and biostatistical strategies have been utilized to process raw/unfiltered medical survey data. The expansion of extreme value statistics from the univariate to the bivariate situation meets with numerous challenges. First, the univariate extreme value types theorem cannot be directly extended to the bivariate (2D) case,—not to mention challenges with system dimensionality higher than 2D. Assessing outbreak risks of future outbreaks in any nation/region of interest. Existing bio-statistical approaches do not always have the benefits of effectively handling large regional dimensionality and cross-correlation between various regional observations. These methods deal with temporal observations of multi-regional phenomena. Apply contemporary, novel statistical/reliability techniques directly to raw/unfiltered clinical data. The current study outlines a novel bio-system hazard assessment technique that is particularly suited for multi-regional environmental, bio, and public health systems, observed over a representative period. With the use of the Gaidai multivariate hazard assessment approach, epidemic outbreak spatiotemporal risks may be properly assessed. Based on raw/unfiltered clinical survey data, the Gaidai multivariate hazard assessment approach may be applied to a variety of public health applications. The study's primary finding was an assessment of the risks of epidemic outbreaks, along with a matching confidence range. Future global COVID-19/severe acute respiratory syndrome coronavirus 2 (SARS-COV2) epidemic risks have been examined in the current study; however, COVID-19/SARS-COV2 infection transmission mechanisms have not been discussed.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 7-8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202400027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a kosmotrope (Na2CO3) and chaotrope (NaCl) in chemometric optimization of aqueous two-phase extraction of bioactive compounds in Hypoxis iridifolia","authors":"Rangani Tracy Lukheli,&nbsp;Nikita Tawanda Tavengwa,&nbsp;Tebogo Mphatlalala Mokgehle","doi":"10.1002/ansa.202400006","DOIUrl":"https://doi.org/10.1002/ansa.202400006","url":null,"abstract":"<p>Enterolactone, coumaric acid and vitexin are polyphenolic compounds present in a variety of fruits, vegetables, cereals and plants. These bioactive compounds are in high demand due to their antioxidant property in various tissues and organs. The purpose of this study was to develop a simultaneous extraction method, an aqueous two-phase extraction (ATPE) method, that would enable the extraction of these compounds from <i>Hypoxis iridifolia</i>. This environmentally friendly extraction method only applied water and ethanol as extraction solvents for these analytes from the plant matrix. After phase separation, the analytes were salted-out from the aqueous phase into the organic phase with the aid of a chaotrope (NaCl) or kosmotrope (Na₂CO₃). Thereafter, the analytes were withdrawn by a micro-pipette for analysis on the high-performance liquid chromatography–photodiode array detector. Optimization was conducted using a central composite design, where three parameters were examined which involved percentage ethanol, centrifugation time and salt type. Generally, the optimized conditions for extraction were an ethanol percentage of 100% and a centrifugation time of 10 min, which yielded concentrations of 2942, 23,823 and 8881 mg kg⁻¹ for enterolactone, vitexin and coumaric acid, respectively, in the presence of a kosmotrope. The optimized conditions of extraction in the presence of chaotrope were an ethanol percentage of 66% and a centrifugation time of 10 min with concentrations of 6727, 20,833 and 8618 mg kg⁻¹ for enterolactone, vitexin and coumaric acid, respectively. The ATPE method involving Na₂CO₃ was a better extractant of all the compounds studied relative to that of NaCl. The superior extraction capability of Na₂CO₃ in ATPE could serve as a prototype for the development of efficient extraction methods to meet the high demand for medicinal compounds derived from natural products.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 7-8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202400006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges and recent advances in quantitative mass spectrometry-based metabolomics","authors":"Nathan Ghafari,&nbsp;Lekha Sleno","doi":"10.1002/ansa.202400007","DOIUrl":"10.1002/ansa.202400007","url":null,"abstract":"<p>The field of metabolomics has gained tremendous interest in recent years. Whether the goal is to discover biomarkers related to certain pathologies or to better understand the impact of a drug or contaminant, numerous studies have demonstrated how crucial it is to understand variations in metabolism. Detailed knowledge of metabolic variabilities can lead to more effective treatments, as well as faster or less invasive diagnostics. Exploratory approaches are often employed in metabolomics, using relative quantitation to look at perturbations between groups of samples. Most metabolomics studies have been based on metabolite profiling using relative quantitation, with very few studies using an approach for absolute quantitation. Using accurate quantitation facilitates the comparison between different studies, as well as enabling longitudinal studies. In this review, we discuss the most widely used techniques for quantitative metabolomics using mass spectrometry (MS). Various aspects will be addressed, such as the use of external and/or internal standards, derivatization techniques, in vivo isotopic labelling, or quantitative MS imaging. The principles, as well as the associated limitations and challenges, will be described for each approach.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 5-6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141473392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted and untargeted metabolomics and lipidomics in dried blood microsampling: Recent applications and perspectives","authors":"Pauline Couacault,&nbsp;Dennisse Avella,&nbsp;Sara Londoño-Osorio,&nbsp;Ana S. Lorenzo,&nbsp;Ana Gradillas,&nbsp;Olli Kärkkäinen,&nbsp;Elizabeth Want,&nbsp;Michael Witting","doi":"10.1002/ansa.202400002","DOIUrl":"10.1002/ansa.202400002","url":null,"abstract":"<p>Blood microsampling (BµS) offers an alternative to conventional methods that use plasma or serum for profiling human health, being minimally invasive and cost effective, especially beneficial for vulnerable populations. We present a non-systematic review that offers a synopsis of the analytical methods, applications and perspectives related to dry blood microsampling in targeted and untargeted metabolomics and lipidomics research in the years 2022 and 2023. BµS shows potential in neonatal and paediatric studies, therapeutic drug monitoring, metabolite screening, biomarker research, sports supervision, clinical disorders studies and forensic toxicology. Notably, dried blood spots and volumetric absorptive microsampling options have been more extensively studied than other volumetric technologies. Therefore, we suggest that a further investigation and application of the volumetric technologies will contribute to the use of BµS as an alternative to conventional methods. Conversely, we support the idea that harmonisation of the analytical methods when using BµS would have a positive impact on its implementation.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 5-6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202400002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141344304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection and association of 12.1 kb deletion within the high mobility AT-hook 2 gene in the Netherlands dwarf rabbit (Oryctolagus Cuniculus)","authors":"Tai Duc Nguyen,&nbsp;Lam Van Dang,&nbsp;Phuong Nhu Nguyen Tran,&nbsp;Dai Van Nguyen,&nbsp;Anh Phu Nam Bui","doi":"10.1002/ansa.202300050","DOIUrl":"10.1002/ansa.202300050","url":null,"abstract":"<p>Rabbits are mainly bred for human consumption and medical research. However, it has been recently showed that several rabbit breeds are also kept as pets for human leisure. The Netherlands dwarf rabbit is currently in the immense interest of many Vietnamese customers due to its personality and miniature stature. However, 12.1 kb deletion from position 44,709,089 to 44,721,236 bp in the high mobility AT-hook 2 (HMGA2) gene on chromosome 4 was identified as the structural variant causing dwarfism and altered craniofacial development in this breed. It has been documented that HMGA2 plays an important role in regulating growth and individuals with genotype HMGA2 <i>del/del</i> are fatal several days after birth. Despite the economically high value of the Netherlands dwarf rabbit, there has been no study on the genetic survey of lethal alleles in this breed in Vietnam. The aim of this study is to develop a fast and reliable method to screen the frequency of lethal alleles of HMGA2 in the South of Vietnam. Rabbit saliva was collected, and DNA extraction was followed. Multiplex polymerase chain reaction (PCR) with three primers was optimized and performed to detect the presence of 12.1 kb deletion within the HMGA2 sequence. Our data showed that the 12.1 kb deletion in the Netherlands dwarf rabbit population was detected by our optimized multiplex PCR. In 100 rabbit animals, 34 and 16 individuals were homozygous wild type (<i>+/+</i>) and homozygous mutant (del/del), respectively, while 50 rabbits were heterozygous. The frequency of HMGA2 lethal allele carrier was 66% (66/100 individuals). Our results indicated that we successfully developed a fast, accurate multiplex PCR to detect carrier individuals. Verification of the genotypes was followed by sequencing. We recommend implementing our multiplex PCR procedure in genetic selection for carrier and homozygous wild-type animals in the mating scheme to prevent the lethality of the rabbit offspring. Additionally, awareness should be raised among rabbit breeders to monitor the genetic makeup of the Netherlands dwarf rabbit populations. However, due to the limitation of the sample size, more samples should be taken in future studies to obtain the genetic frequency of the HMGA2 lethal allele more accurately.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"5 7-8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.202300050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141273279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}