Analytical science advances最新文献

{"title":"Schiff Base-UiO66 Composite-based Dispersive Micro Solid-Phase Extraction of Pesticides From Fruit Juices Combined With Dispersive Liquid-Liquid Microextraction Prior to Gas Chromatography-Flame Ionisation Detection","authors":"Behnam Hosseininezhad,&nbsp;Mahdi Bomorovat,&nbsp;Mahboob Nemati,&nbsp;Mir Ali Farajzadeh,&nbsp;Ramin Atazadeh,&nbsp;Mohammad Reza Afshar Mogaddam","doi":"10.1002/ansa.70102","DOIUrl":"10.1002/ansa.70102","url":null,"abstract":"<p>A dispersive micro solid-phase extraction method was introduced using a Schiff base-UiO66 nanocomposite as the adsorbent in the extraction of multi-class pesticide residues in pear, cherry, grape, and apricot juices. The extraction procedure was hyphenated with a liquid-phase microextraction procedure, providing a high preconcentration factor and sensitive detection limit. During the extraction procedure, a small amount of the sorbent was added to the sample solution and dispersed via vortexing. Then, the sorbent particles were separated, and the adsorbed analytes were eluted by an appropriate water-miscible organic solvent, which was subsequently used for the preconcentration of the analytes in the following microextraction step. Gas chromatography-flame ionisation detector was utilised as a determination system. All effective factors were evaluated to obtain their optimum values, and then the method was validated. Low limits of detection (0.13–0.27 ng mL⁻¹) and low limits of quantification (0.43–0.89 ng mL⁻¹), acceptable extraction recoveries (59%–71%), high enrichment factors (295–355) and good relative standard deviations (≤10.1%) were the advantages of the method. Fortunately, application of the method on the studied samples showed that the fruit juices were free of the analytes.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"7 2","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13432960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148671572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a Circular RNA as a Potential Diagnostic and Prognostic Biomarker in Breast Cancer Through Integrated Bioinformatic and Experimental Analyses","authors":"MohamadReza Hashemi,&nbsp;Pejman Morovat,&nbsp;Azin Khoshghiafeh,&nbsp;Novin Nikbakhsh,&nbsp;Mohamadreza Ahmadifard","doi":"10.1002/ansa.70098","DOIUrl":"https://doi.org/10.1002/ansa.70098","url":null,"abstract":"<p>Breast cancer (BC), which has complex molecular subgroups that contribute to a range of clinical outcomes, is still one of the top causes of morbidity and death among women globally. This challenge necessitates ongoing research to improve early detection, treatment strategies, and patient outcomes. Recent research has shown that circular RNAs (circRNAs) influence gene expression through various mechanisms, particularly by functioning as competing endogenous RNAs (ceRNAs). Moreover, numerous circRNAs have been implicated in the initiation and progression of various tumor types. Yet, the expression and functional roles of multiple circRNAs remain largely unexplored in specific cancers. In this study, circRNA expression data from three Gene Expression Omnibus datasets were analyzed to identify candidate circRNAs associated with BC. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) was performed to validate the candidate circRNAs in tissue samples from 24 BC patients. Using bioinformatic analyses, downstream target microRNAs and messenger RNAs of these circRNAs were probed for the construction of a ceRNA regulatory network. A combined number of 40 distinct circRNAs with differential expression were identified, among which hsa_circ_0000231 and hsa_circ_0011385 were selected based on their novelty and potential to function as ceRNAs, and a corresponding ceRNA network was built. RT-qPCR revealed that hsa_circ_0000231 is significantly upregulated in BC tissues compared to adjacent non-cancerous tissues. Additionally, based on bioinformatic investigations, hsa_circ_0000231/hsa-miR-5683/cyclin B1 regulatory axis was predicted. By combining cross-dataset computational screening with targeted experimental validation and diagnostic accuracy assessment, this study presents a systematic framework for translating high-throughput RNA data into quantifiable biomarker candidates with potential clinical relevance. These findings may contribute to the identification of a novel diagnostic and prognostic biomarker in BC.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"7 2","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.70098","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148616298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Biochemical Analytes Stability: The Role of Centrifugation and Storage Conditions in the Preanalytical Phase","authors":"Zivile Jacike,&nbsp;Rugile Skripkauske,&nbsp;Zivile Sauciuniene,&nbsp;Ema Rajackaite,&nbsp;Paulius Kibisa,&nbsp;Vaiva Lesauskaitė,&nbsp;Ingrida Grabauskyte,&nbsp;Gajdová Marie,&nbsp;Ramune Sepetiene","doi":"10.1002/ansa.70100","DOIUrl":"10.1002/ansa.70100","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>The preanalytical phase represents a critical component of laboratory testing, encompassing all procedures prior to sample analysis and exerting a substantial influence on the quality and reliability of laboratory results. To evaluate changes in the concentrations of commonly measured biochemical analytes in blood samples subjected to different preanalytical conditions, including centrifugation duration, storage temperature and storage time. Blood samples obtained from 20 individuals (960 measurements) were analysed for glucose, albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglycerides (TG), potassium (K), sodium (Na) and chloride (Cl). Samples were centrifuged for 5, 10 and 15 min, analysed under optimal conditions (within 2 h of collection), and subsequently reassessed after storage for 2, 4, 8, 10 and 14 days at +23, 4 and −20°C. Statistical analysis was performed using the Kruskal–Wallis test. Storage temperature significantly affected HDL, TG, ALT, AST, glucose, albumin, LDL, and TC (<i>p</i> &lt; 0.001) concentrations, with higher HDL and TG concentrations at +23°C ([1.71(1.12-2.50)]mmol/l] and [1.82(0.73-10.19)]mmol/l respectively) and significant AST and ALT concentration decrease at 23°C ([16.0 (4-60)]U/l and [10.0(2-95)]U/l respectively). Storage duration influenced TC, HDL, TG, albumin, ALT, and AST concentrations (<i>p</i> &lt; 0.001). Centrifugation time had a significant effect only on serum chloride and glucose concentrations (<i>p</i> = 0.018, <i>p</i> = 0.025 respectively). Most biochemical analytes were significantly influenced by storage temperature and duration, whereas centrifugation time had a comparatively limited effect.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"7 2","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13401993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148594972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Phytochemical Characterisation, Pharmacological Evaluation and Computational Investigation of Acampe papillosa Stem Extract","authors":"Qurratul Ain Sadia,&nbsp;Sadia Tamanna Tamim,&nbsp;Faruq Mohammed Tashriq,&nbsp;Nasir Uddin,&nbsp;Fatematus Zhohara Alam Hima,&nbsp;Ulfat Sobha Surat,&nbsp;Mily Khastagir,&nbsp;Muhammad Abdul Jalil,&nbsp;Md. Hossain Rasel,&nbsp;Arman Ullah Rafi,&nbsp;S. M. Moazzem Hossen","doi":"10.1002/ansa.70097","DOIUrl":"10.1002/ansa.70097","url":null,"abstract":"<p><i>Acampe papillosa</i>, an orchid species traditionally utilised as an herbal medicine, has yet to be extensively researched for its potential pharmacological properties. This study examined the potential for anti-anxiety, sedative, anti-inflammatory and antipyretic effects of methanol extract of the stem of <i>A. papillosa</i> (APSME) through in vivo and in silico approaches. A dose of 200 and 400 mg/kg was administered to Swiss albino rodents. When examined in the elevated plus maze, APSME-treated mice spent significantly longer periods of time in the open arms of the maze at both dosages than control animals (<i>p</i> &lt; 0.001), indicating an anxiolytic effect of APSME. The sedative effects of APSME were assessed by measuring locomotor activity using an open field test and a hole cross test, with both methods showing a significant decrease in locomotion as APSME doses increased from 200 to 400 mg. Following treatment of rodents with carrageenan-induced paw oedema using APSME (400 mg/kg), significant reductions in the thickness of the paw were observed at 4 h post-administration compared to control groups (<i>p</i> &lt; 0.001), indicating strong anti-inflammatory activity. Antipyretic effects of APSME were measured using a brewer's yeast-induced pyrexia animal model and were accompanied by statistically significant reductions in rectal temperature at 21 h post-treatment (98.04 ± 0.05°F). Molecular docking and PASS prediction further support these findings by showing favourable interactions between the plant phytochemicals and relevant biological targets. Overall, the results suggest that <i>A. papillosa</i> could be a promising natural source for developing treatments for anxiety, inflammation, fever and related conditions.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"7 2","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13395471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148581627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design-Assisted Chemometric UV Spectrophotometric Determination of Candesartan Cilexetil, Chlorthalidone and Amlodipine Using Principal Component Regression, Partial Least Squares and Genetic Algorithm-Partial Least Squares Models","authors":"Khanda F. M. Amin,&nbsp;Samar H. Elagamy,&nbsp;Reem H. Obaydo,&nbsp;Hayam M. Lotfy","doi":"10.1002/ansa.70101","DOIUrl":"10.1002/ansa.70101","url":null,"abstract":"<p>This study presents a sustainable chemometric-assisted UV spectrophotometric strategy for the simultaneous determination of candesartan cilexetil (CAN), chlorthalidone (CTL) and amlodipine (AML) in laboratory-prepared mixtures and commercial pharmaceutical formulations. Owing to the extensive spectral overlap of the three drugs in the UV region, conventional spectrophotometric methods are inadequate for their direct simultaneous analysis. To address this challenge, multivariate calibration models based on principal component regression (PCR), partial least squares (PLS) and genetic algorithm-optimized partial least squares (GA-PLS) were developed, enabling accurate quantification without prior separation or complex sample preparation. A design of experiments (DoE) approach was employed to construct the calibration set, while an independent validation set was generated using orthogonal array-based Latin hypercube sampling (OALHS) to ensure robust external validation across the concentration domain. The GA-PLS model enhanced predictive performance through effective wavelength selection, reducing spectral redundancy and improving model robustness. Furthermore, variable importance in projection (VIP) analysis was employed to identify the most influential spectral variables and provide insight into the spectral regions contributing to analyte quantification. The developed models exhibited excellent analytical performance, characterized by low calibration errors (root mean square error of calibration [RMSEC] &lt; 0.30), strong predictive ability and satisfactory external validation results (RMSEP = 0.2278–0.4419; RRMSEP = 0.1558–0.3978), with recoveries ranging from 99.0% to 100.0%. The sustainability of the proposed methodology was assessed using the multi-colour assessment (MA) tool according to white analytical chemistry principles, yielding a high whiteness score and demonstrating superior environmental performance compared with conventional chromatographic methods. In addition, the graphical layout tool for analytical chemistry evaluation (GLANCE) visualization framework provided a comprehensive graphical assessment of analytical performance and sustainability metrics. The proposed chemometric strategy offers a rapid, reliable, cost-effective and environmentally friendly alternative for routine quality control of multicomponent pharmaceutical formulations.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"7 2","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148564066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Laboratory Innovations: Nanoscale Detection of Chloramphenicol Using Nanomaterial Biosensors","authors":"Nava Moghadasian Niaki,&nbsp;Reza Kheradmand,&nbsp;Seyedeh Giso Akbarian,&nbsp;Elham Shaterian,&nbsp;Hamed Shaterian,&nbsp;Ahmad Mobed","doi":"10.1002/ansa.70093","DOIUrl":"10.1002/ansa.70093","url":null,"abstract":"<p>Chloramphenicol (CAP) is an important broad-spectrum antibiotic used to treat severe bacterial infections, but its clinical application is limited by serious toxic effects, including aplastic anaemia and bone marrow suppression. These care concerns underscore the need for fast, sensitive and reliable analytical methods for CAP detection in biological and food samples. In recent years, nanomaterial-based biosensors have emerged as encouraging tools for this purpose, offering enhanced sensitivity, selectivity and analytical performance. This review examines recent advances in nanoscale detection of CAP using nanomaterial biosensors, with specific emphasis on nanomaterial-based electrochemical platforms. The exceptional physicochemical properties of these materials, including enhanced electron transfer, large surface area, catalytic activity and signal amplification, have contributed to the development of highly effective sensing systems. These biosensors have shown strong potential for rapid analysis and trace detection in complex matrices. Moreover, several challenges remain before these platforms can be fully translated into routine clinical and regulatory use. Subjects such as matrix effects, reproducibility, stability and validation against reference methods require further attention. This review critically discusses current progress, highlights the limitations of existing approaches and outlines future directions for the development of practical laboratory-based biosensing approaches for CAP detection.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"7 2","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13345455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148414199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Pharmacokinetics of Six Bioactive Constituents From Danggui Jixueteng Decoction in Normal and Myelosuppressive Rats","authors":"Mingxin Guo,&nbsp;Yingjie Liu,&nbsp;Zhiqiang Hu,&nbsp;Xiayu Liu","doi":"10.1002/ansa.70096","DOIUrl":"https://doi.org/10.1002/ansa.70096","url":null,"abstract":"<p>This study compared the pharmacokinetics of six constituents from Danggui Jixueteng Decoction in normal versus carboplatin-induced myelosuppressive rats. A rapid, sensitive ultra-high performance liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous quantification of six diverse constituents (8-<i>O</i>-debenzoylpaeoniflorin, ferulic acid, calycosin, albiflorin, oxypeucedanin hydrate and neocryptotanshinone) in rat plasma, and applied for the first time to compare pharmacokinetics under normal and diseased states. Male Sprague-Dawley rats received oral decoction (12.42 g/kg daily, 7 days) after myelosuppression induction by intraperitoneal carboplatin. Serial blood samples were collected after the final dose and analysed. In model rats, systemic exposure to calycosin increased significantly, with elevated area under the curve (AUC_0–∞) and maximum concentration (<i>C</i>_max) (<i>p</i> &lt; 0.05), along with a shortened <i>T</i>_max. 8-<i>O</i>-Debenzoylpaeoniflorin showed a trend towards increased exposure, though the differences did not reach statistical significance. Conversely, neocryptotanshinone showed reduced AUC_0–∞ and <i>C</i>_max, delayed <i>T</i>_max, and a significantly shorter elimination half-life (<i>p</i> &lt; 0.05). Ferulic acid exposure remained stable, but its half-life shortened significantly (<i>p</i> &lt; 0.05). Albiflorin and oxypeucedanin hydrate were minimally affected. Myelosuppression selectively alters the in vivo disposition of the formula constituents, supporting its use as an adjunctive therapy during chemotherapy.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"7 2","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.70096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148358089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic Mapping of Greek Olive Leaves by Untargeted NMR-Based Profiling and LC–HRMS Dereplication","authors":"Mariacaterina Lianza,&nbsp;Stavros Beteinakis,&nbsp;Vasileios Siderakis,&nbsp;Panagiotis Stathopoulos,&nbsp;Emmanuel Hatzakis,&nbsp;Maria Halabalaki","doi":"10.1002/ansa.70094","DOIUrl":"https://doi.org/10.1002/ansa.70094","url":null,"abstract":"<p>Olive tree leaves are phytochemically rich yet insufficiently valorised in terms of systematic cultivar-based characterisation and exploitation, and are an abundant olive industry side-product. This study represents the first large-scale metabolome mapping based on 340 leaf extracts from five major Greek cultivars (Amfissis, Koroneiki, Manaki, Lianolia-Kerkyra and Thasou) collected across diverse regions, agronomic practices, harvest periods and years. An integrated analytical workflow combining untargeted ¹H NMR profiling and LC–HRMS dereplication was applied for the first time at this scale. NMR provided a quantitative and highly reproducible overview of the core metabolome and served as the basis for multivariate statistical analysis, while HRMS enabled confident structural annotation of 62 metabolites, including secoiridoids, flavonoids, phenolic acids, triterpenoids and fatty acids. Multivariate data analysis (PCA and OPLS-DA) revealed that cultivar identity constitutes the dominant driver of metabolic variation, exceeding the influence of environmental, agronomic and temporal factors. Pentacyclic triterpenic acids, that are, oleanolic and maslinic acids, emerged as robust, genotype-dependent chemotaxonomic markers, outperforming the widely used oleuropein, whose abundance is highly sensitive to environmental and agronomic factors. Flavonoids (apigenin, luteolin, quercetin) and the sugar alcohol mannitol displayed cultivar-specific patterns consistent with differential stress adaptation strategies rather than strict taxonomic control. Amfissis was characterised by the highest and most stable triterpenoid levels, Koroneiki/Thasou by elevated flavonoid levels, while Koroneiki/Manaki showed the highest relative concentration of oleuropein and mannitol. Overall, this integrated NMR/LC–HRMS approach establishes a robust framework for olive leaf cultivar authentication and supports genotype-driven valorisation of this agricultural side-product, enabling the development of standardised, cultivar-specific extracts for phytotherapeutic, nutraceutical and cosmetic applications.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"7 2","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.70094","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148358177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biphasic Extraction of Selected Heavy Metals From Corn With the Aid of Kosmotropic Salts","authors":"Vhuhwavho Masindi,&nbsp;Shirley Selahle,&nbsp;Tebogo Mokgehle","doi":"10.1002/ansa.70095","DOIUrl":"https://doi.org/10.1002/ansa.70095","url":null,"abstract":"<p>This study investigated the use of aqueous two-phase extraction (ATPE) with kosmotropic salts for the extraction of toxic heavy metals from maize. Heavy metals, such as cadmium (Cd), chromium (Cr) and iron (Fe), are harmful to human health because they accumulate in the food chain and may cause kidney damage, cancer and nervous system problems. This study tested copper sulphate (CuSO₄) and magnesium sulphate (MgSO₄) as extracting agents under different conditions of salt concentration, solvent volume and maize mass. The extracted metals were analysed using atomic absorption spectroscopy (AAS). Results showed that CuSO₄ was significantly more effective than MgSO₄, with the highest extraction achieved for cadmium, followed by chromium, and the least for iron. Response surface methodology (RSM) analysis confirmed that medium salt concentrations (30%–35%) and solvent volumes of 7–8 mL gave the best results. Fitting central composite design response model to the data generated a quadratic model with fits of <i>R</i>² = 0.90, <i>R</i>² = 0.90 and <i>R</i>² = 0.92 for Fe(I), Cr(III), and Cd, respectively. As we use environmental method (ATPE) to minimize those heavy metals (Cr(III), Cd(II) and Fe(I)) from corn, then corn can still be edible after exposure to ATPE because only water and ethanol are used. Comparison with international food safety standards revealed that Fe and Cr levels in maize were well within World Health Organization (WHO) limits. However, Cd(II) was found at 0.328 mg kg⁻¹, which is higher than both the WHO limit (0.2 mg kg⁻¹) and the stricter South African standard (0.05 mg kg⁻¹). This indicates a serious food safety risk. The study concludes that ATPE with CuSO₄ is a promising, eco-friendly and low-cost method for extracting heavy metals from maize. However, further optimization and the use of complementary agents are recommended to improve cadmium extraction and ensure safe consumption.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"7 2","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ansa.70095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148358178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Two New Flavonoid Glycosides From <i>Zanha golungensis</i> Hiern With COX Inhibition Activity: Structural Elucidation, DFT Calculations, In Vitro and Molecular Docking Assessment.","authors":"Jean Zeuikena, Romeo Toko Feunaing, Alfred Ngenge Tamfu, Abel Joel Yaya Gbaweng, Celine Henoumont, Sophie Laurent, El Hassane Anouar, Emmanuel Talla","doi":"10.1002/ansa.70092","DOIUrl":"10.1002/ansa.70092","url":null,"abstract":"<p><p><i>Zanha golungensis</i> is used as herbal medicine for a variety of ailments. In this study, flavonoids, including two new kaempferide polyglycosides, namely, kaempferide-3-<i>O</i>-[α-l-rhamnopyranosyl-(1 <math> <mrow><mrow><mo>→</mo> <mn>2</mn></mrow> </mrow> </math> )-α-l-rhamnopyranosyl-(1 <math> <mrow><mrow><mo>→</mo> <mn>6</mn></mrow> </mrow> </math> )]-β-d-glucopiranoside-7-<i>O</i>-α-l-rhamnopiranoside (<b>1</b>) and kaempferide-3-<i>O</i>-[4-<i>O</i>-acetyl-α-l-rhamnopyranosyl-(1 <math> <mrow><mrow><mo>→</mo> <mn>2</mn></mrow> </mrow> </math> )-α-l-rhamnopyranosyl-(1 <math> <mrow><mrow><mo>→</mo> <mn>6</mn></mrow> </mrow> </math> )]-β-d-glucopiranoside-7-<i>O</i>-α-l-rhamnopiranoside (<b>2</b>) alongside known compounds (-)-epicathecin (<b>3</b>), procyanidin A2 (<b>4</b>) and procyanidin B2 (<b>5</b>), were isolated and characterized using ESI-TOF-MS and NMR. The compounds exhibited good cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) inhibitions with high percentage inhibitions at 20 µg/mL. Compound was most active with percentage inhibitions of 74.3 ± 1.2 and 78.6 ± 1.5 on COX-1 and COX-2, respectively, compared to Cy-3-coumSamb-5-Glc (standard) with percentage inhibitions of 68.3 ± 1.1 and 70.0 ± 1.4 on COX-1 and COX-2, respectively. Density functional theory (DFT) calculations were used in predicting the electronic and molecular properties of isolated compounds, notably optimized geometries and molecular electrostatic potentials, which all correlated with highest occupied molecular orbital (HOMO)-lowest unoccupied molecular orbital (LUMO) energies. Molecular docking predictions indicated favourable and thermodynamically stable complexes between the compounds and COX-2 receptor sites revealed by binding affinities and negative binding energies. The results show the potential anti-inflammatory, antipyretic and anticancer potential drug design of the isolated compounds.</p>","PeriodicalId":93411,"journal":{"name":"Analytical science advances","volume":"7 1","pages":"e70092"},"PeriodicalIF":5.4,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13262979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148254906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}