BMC Biology最新文献

{"title":"Brain miR-137 governs growth and development via GH/IGF-1 signaling.","authors":"Keng-Mao Liao, Wei-Lun Hsu, Wan-Yi Huang, Wei-Jia Luo, Jung-Hsuan Chang, Sung-Liang Yu, Pan-Chyr Yang, Kang-Yi Su","doi":"10.1186/s12915-025-02306-8","DOIUrl":"10.1186/s12915-025-02306-8","url":null,"abstract":"<p><strong>Background: </strong>Brain-enriched miR-137 is highly associated with neuropsychiatric disorders and neural development. Although complete loss of miR-137 that leads to postnatal lethality had been addressed in mice, the underlying mechanism particularly related to growth and development remains unknown.</p><p><strong>Results: </strong>MiR-137-deficient mice (Mir137^-/-) exhibited postnatal lethality, severe growth retardation, osteoporosis, fat atrophy, and hypothermia. Despite comparable serum growth hormone (GH) levels, IGF-1 levels in both liver and serum were significantly reduced, with compensatory upregulation of IGF-1 receptor expression in major organs. Reduced IGF-1 levels were not due to defects in GH secretion by the pituitary nor GH responsiveness of hepatocytes. Instead, impaired in vivo GH-induced p-STAT5 signaling suggested GH resistance in Mir137^-/-. Conditional deletion of Mir137 in the nervous system, but not in the liver, showed similar results, confirming the brain-specific role of miR-137. Transcriptomic analyses revealed that differentially expressed genes in the brain were enriched in development and neurogenesis while those in the liver showed diverse and less enrichments. IGF-1 reduction caused by miR-137 deficiency emerged as a central factor impacting the cell proliferation network to systemic growth.</p><p><strong>Conclusions: </strong>This study underscores the critical role of miR-137 in failure to thrive through regulation of the GH/IGF-1 axis and supports the use of MiR137^-/- as a disease model for GH resistance. Given the conserved miR-137 sequences between mice and humans, further human studies or clinical trials may validate its potential as a biomarker and therapeutic target for growth retardation.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"197"},"PeriodicalIF":4.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep learning-based dipeptidyl peptidase IV inhibitor screening, experimental validation, and GaMD/LiGaMD analysis.","authors":"Yi He, Yan Zhang, Minghao Liu, Jiaying Li, Wannan Li, Weiwei Han","doi":"10.1186/s12915-025-02295-8","DOIUrl":"10.1186/s12915-025-02295-8","url":null,"abstract":"<p><strong>Background: </strong>Dipeptidyl peptidase-4 (DPP4) is considered a crucial enzyme in type 2 diabetes (T2D) treatment, targeted by inhibitors due to its role in cleaving glucagon-like peptide-1 (GLP-1). In this study, a novel DPP4 inhibitor screening strategy was developed, which significantly improved screening accuracy.</p><p><strong>Results: </strong>In this study, a DPP4 inhibitor screening method was developed, integrating receptor-based ConPLex, ligand-based KPGT, and molecular docking to enhance screening accuracy. Using this approach, four potential drugs were identified from the FDA database, achieving a 100% hit rate. Among these, Isavuconazonium demonstrated the highest inhibitory activity (IC₅₀ = 6.60 µM). Furthermore, a user-friendly server, DPP4META, was established to predict IC₅₀ values for DPP4 inhibitors. The binding and dissociation mechanisms of these drugs with DPP4 were further examined through Gaussian accelerated Molecular Dynamics (GaMD) and ligand Gaussian accelerated Molecular Dynamics (LiGaMD), revealing strong correlations with IC₅₀ values. Additionally, a Python-based toolkit, pymd, was developed to facilitate protein-compound binding analysis.</p><p><strong>Conclusions: </strong>Our study offers a robust approach and valuable insights for the development of DPP4 inhibitors, providing an effective means to investigate the binding and dissociation mechanisms between proteins and compounds.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"173"},"PeriodicalIF":4.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12211148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KLF13 promotes ferroptosis and chemosensitivity in lung adenocarcinoma.","authors":"Haochun Shi, Binyang Pan, Gujie Wu, Jiaqi Liang, Yunyi Bian, Guangyao Shan, Shencheng Ren, Guoshu Bi, Cheng Zhan, Weigang Guo","doi":"10.1186/s12915-025-02303-x","DOIUrl":"10.1186/s12915-025-02303-x","url":null,"abstract":"<p><strong>Background: </strong>Ferroptosis, a form of cell death reliant on iron metabolism dysregulation and lipid peroxidation, has emerged as a promising target for improving tumor drug resistance. This study aims to unveil the underlying molecular mechanisms of Kruppel-like factor 13 (KLF13) in ferroptosis and chemotherapy sensitivity in lung adenocarcinoma (LUAD).</p><p><strong>Results: </strong>We conducted RNA sequencing on lung adenocarcinoma cells treated with ferroptosis inducers and found that the expression level of KLF13 changed during ferroptosis, suggesting its potential involvement in this process. Subsequently, we generated stable cell lines overexpressing and knocking down KLF13. Cytotoxicity assays and reactive oxygen species (ROS) detection demonstrated that overexpression of KLF13 promoted ferroptosis induced by IKE and RSL3 in LUAD cells, whereas silencing KLF13 inhibited ferroptosis. Furthermore, overexpression of KLF13 enhanced the chemotherapeutic efficacy of cisplatin and pemetrexed. RNA-seq, qPCR, and western blot experiments revealed that KLF13 downregulated the RNA and protein expression of GPX4. ChIP assays and dual-luciferase reporter gene assays indicated that KLF13 directly bound to the promoter region of GPX4, inhibiting transcriptional activity of GPX4. Additionally, overexpression and knockdown of GPX4 could reverse the effects of KLF13 on ferroptosis and chemosensitivity. These findings were further confirmed through immunohistochemical staining and animal experiments.</p><p><strong>Conclusions: </strong>Our study reveals that KLF13 promotes ferroptosis in LUAD by inhibiting GPX4, thereby enhancing sensitivity to chemotherapy drugs. Overall, targeting KLF13 may contribute to developing new therapeutic strategies for LUAD.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"178"},"PeriodicalIF":4.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12210755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144538967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering gut microbiome patterns from host preferences and microbial interactions in healthy Korean individuals.","authors":"Seungpyo Hong, Mi Young Lim, Won-Hyong Chung, Ji-Hee Shin, Young-Do Nam","doi":"10.1186/s12915-025-02291-y","DOIUrl":"10.1186/s12915-025-02291-y","url":null,"abstract":"<p><strong>Background: </strong>The gut microbiome is crucial for human health maintenance and disease development, yet limited understanding of its structure and maintenance hinders effective microbiome-based health improvement strategies. We investigated gut microbiome compositional patterns in healthy Koreans (n = 890), identifying six clusters (I-VI) with unique compositions and host preferences.</p><p><strong>Results: </strong>Each cluster had a distinct topological structure within the microbial interaction network, underscoring its diverse roles in maintaining microbial communities. Cluster II, predominated by Bacteroides and Faecalibacterium, was consistently found across individuals and centrally located within the microbial interaction network. Cluster III, mainly composed of Oscillospira and Coprococcus, and IV, dominated by Enterobacteriaceae and Bacteroides fragilis, demonstrated mutually exclusive relationships, reflecting affinities for host clusters with varied dietary patterns and microbial diversity. Clusters V and VI linked different microbial clusters, and cluster I had separate subcommunities.</p><p><strong>Conclusions: </strong>This study reveals intricate structures and interactions within microbial communities, offering insights into the gut microbiome ecology and guiding health enhancement strategies.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"185"},"PeriodicalIF":4.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12220063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The hierarchical folding dynamics of topologically associating domains during early embryo development.","authors":"Xuemei Bai, Xiaohan Tang, Yuyang Wang, Shutong Yue, Xiang Xu, Pengzhen Hu, Jingxuan Xu, Yaru Li, Junting Wang, Huan Tao, Yang Zheng, Bijia Chen, Mengge Tian, Lin Lin, Ruiqing Wang, Yu Sun, Chao Ren, Xiaochen Bo, Hao Li, Hebing Chen, Meisong Lu","doi":"10.1186/s12915-025-02259-y","DOIUrl":"10.1186/s12915-025-02259-y","url":null,"abstract":"<p><strong>Background: </strong>Recent research has indicated a close connection between the three-dimensional (3D) structure of chromatin and early embryo development, with precise higher-order chromatin folding playing a significant role in mediating gene expression. However, the specific role of 3D genomic hierarchical structure and its dynamics in early embryo development remains largely unknown.</p><p><strong>Results: </strong>In this study, we examined the hierarchical topological association domain (TAD) during early embryo development and its relationship with zygotic gene activation (ZGA), gene expression, and chromatin accessibility to gain a better understanding of the dynamics of TAD nesting levels during this developmental stage. Our findings show that ZGA precedes the establishment of hierarchical TAD, leading to widespread gene expression, an increase in the percentage of high-level TAD structures, and enhanced chromatin accessibility at higher hierarchical levels. Additionally, we utilized a deep neural network to investigate the formation of TAD boundaries and found that histone H3 lysine 4 trimethylation (H3K4me3) and histone H3 lysine trimethylation (H3K27me3) are key features in the establishment of TAD boundaries. Furthermore, we observed heterogeneous dynamics of hierarchical TAD among different species.</p><p><strong>Conclusions: </strong>Overall, our study sheds light on the folding dynamics of hierarchical TADs during early embryo development and underscores their close relationship with transcriptional programs.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"175"},"PeriodicalIF":4.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12210587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strain sharing and mobile genetic elements shape the interconnected resistomes of Campylobacter coli in Brazil.","authors":"Ana Beatriz Garcez Buiatte, Leticia Roberta Martins Costa, Stephanie S R Souza, Nicole I Zac Soligno, Roberta Torres de Melo, Paulo Marcel Armendaris, Daise Aparecida Rossi, Cheryl P Andam","doi":"10.1186/s12915-025-02283-y","DOIUrl":"10.1186/s12915-025-02283-y","url":null,"abstract":"<p><strong>Background: </strong>Campylobacter coli is a ubiquitous commensal in the gut of birds and mammals. It causes acute gastroenteritis in humans when contaminated livestock meat is consumed and can be fatal in vulnerable individuals. Here, we aim to characterize the population genomic structure and antimicrobial resistance (AMR) dissemination of C. coli in Brazil contextualized against a South American background.</p><p><strong>Results: </strong>We analyzed 32 newly sequenced short-read genomes from Brazil and 158 previously sequenced genomes from different locations in South America. Clonal complex CC828, which is known to be predominant in agriculture and human diseases, accounted for 82.4% of the Brazilian genomes (n = 108). We identified six mutations, 15 acquired genes, and one operon (cmeABC) that were associated with AMR in the Brazilian population. Six AMR determinants (aad9, aph(3')-IIIa, gyrA T86I, 23S A2075G, blaOXA-460, tet(O)) displayed co-occurrence albeit at different paired combinations and probabilities. The Brazilian genomes showed close phylogenetic relationship with those from Chile, Ecuador, and Peru. Across the four countries, AMR determinants associated with putative plasmids or transposable elements included those related to aminoglycosides, streptothricin, tetracyclines, and multidrug resistance.</p><p><strong>Conclusions: </strong>The widespread dissemination of CC828 and their mobile genetic elements shape AMR distribution in C. coli across Brazil. A long-term epidemiological and genomic surveillance system in the country will be useful to determine how campylobacteriosis can be controlled effectively and the scale at which interventions need to be enforced.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"172"},"PeriodicalIF":4.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12210442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The phosphatase PPM1F, a negative regulator of integrin activity, is essential for embryonic development and controls tumor cell invasion.","authors":"Tanja M Grimm, Nina I Dierdorf, Marleen Herbinger, Sarah Baumgärtner, Erik Sontowski, Christoph Paone, Timo Baade, Christof R Hauck","doi":"10.1186/s12915-025-02254-3","DOIUrl":"10.1186/s12915-025-02254-3","url":null,"abstract":"<p><strong>Background: </strong>The Mn²⁺/Mg²⁺-dependent Ser/Thr phosphatase PPM1F was identified to control integrin activity. Furthermore, PPM1F regulates several protein kinases known to be involved in organizing the cytoskeleton and other cellular functions. Therefore, PPM1F appears critical for a multitude of physiological processes.</p><p><strong>Results: </strong>Here, we report the phenotype of ppm1f gene disruption in mice. While heterozygous ppm1f ± mice are viable and fertile, ppm1f-/- mice show severe defects and significant morphological abnormalities in the developing brain and vasculature and abort embryonic development at day E10.5. Isolated ppm1f-/- MEFs or PPM1F-depleted human neuro-epithelial cells display enhanced integrin-dependent cell adhesion, deregulated PAK phosphorylation, and perturbed cell migration. These phenotypes were reversed by re-expression of the wildtype enzyme, but not the phosphatase-inactive PPM1F. In different human tumor cell types, PPM1F expression levels directly correlated with invasive potential, while deletion of PPM1F abrogates tissue invasion.</p><p><strong>Conclusions: </strong>These results highlight the non-redundant role of this enzyme in integrin and PAK regulation and identify PPM1F as a promising target to limit tumor metastasis.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"166"},"PeriodicalIF":4.4,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12180154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Author Correction: Tyrosinase in melanoma inhibits anti-tumor activity of PD-1 deficient T cells.","authors":"Rong Huang, Yingbin Wang, Haitao Teng, Mengjun Xu, Kexin He, Yingzhuo Shen, Guo Guo, Xinyu Feng, Tianhan Li, Binhui Zhou, Marc Bajenoff, Toby Lawrence, Yinming Liang, Liaoxun Lu, Lichen Zhang","doi":"10.1186/s12915-025-02285-w","DOIUrl":"10.1186/s12915-025-02285-w","url":null,"abstract":"","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"165"},"PeriodicalIF":4.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12168257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond venomous fangs: Uloboridae spiders have lost their venom but not their toxicity.","authors":"Xiaojing Peng, Ludwig Dersch, Josephine Dresler, Tim Lüddecke, Tim Dederichs, Peter Michalik, Steve Peigneur, Jan Tytgat, Afrah Hassan, Antonio Mucciolo, Marc Robinson-Rechavi, Giulia Zancolli","doi":"10.1186/s12915-025-02248-1","DOIUrl":"10.1186/s12915-025-02248-1","url":null,"abstract":"<p><strong>Background: </strong>Venom, one of nature's most potent secretions, has played a crucial role in the evolutionary success of many animal groups, including spiders. However, Uloboridae spiders appear to lack venom and capture their prey, unlike venomous spiders, by extensive silk-wrapping and regurgitation of digestive fluids onto the entire prey package. A prevailing hypothesis posits that toxins may have been reallocated from the venom to alternative secretions, like silk or digestive fluids. Yet, whether uloborids have retained venom toxins and the mechanisms underlying prey immobilisation remain unresolved. Here, we employed a multi-disciplinary approach to assess the absence of venom glands in Uloborus plumipes, toxin gene expression and toxicity of digestive proteins.</p><p><strong>Results: </strong>Our findings confirm that U. plumipes lacks a venom apparatus, while neurotoxin-like transcripts were highly expressed in the digestive system. Midgut extract had comparable toxicity levels to that of the venomous Parasteatoda tepidariorum. However, no inhibitory effects on sodium nor potassium channels were observed, indicating a different toxic mechanism.</p><p><strong>Conclusions: </strong>These findings support the hypothesis that Uloboridae spiders have lost their venom apparatus while retaining toxin-like genes. The potent toxicity of their digestive fluids, a trait conserved across spiders, likely compensate for the absence of venom, ensuring effective prey immobilisation and digestion.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"159"},"PeriodicalIF":4.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12164160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell chromatin accessibility profiling reveals regulatory mechanisms and evolution in pig brains.","authors":"Yue Xiang, Saixian Zhang, Yi Huang, Zhuqing Zheng, Jiahui Sun, Qiulin Zhao, Peng Zhou, Xiaolong Qi, Jingjin Li, Fuyang Xiong, Jing Xu, Shengquan Wang, Liangliang Fu, Xinyun Li","doi":"10.1186/s12915-025-02263-2","DOIUrl":"10.1186/s12915-025-02263-2","url":null,"abstract":"<p><strong>Background: </strong>Pig brains serve as a valuable biomedical model for studying brain-related diseases due to their significant structural similarities to the human brain. Furthermore, the long-term domestication and artificial selection of domestic pigs have profoundly shaped their brains, making them an interesting subject for research. However, a comprehensive understanding of the regulatory mechanisms governing pig brain function and their impact on various phenotypes remains elusive due to the high degree of cellular heterogeneity present in the brain.</p><p><strong>Results: </strong>In this study, we profiled 71,798 cells from domestic pig and wild boar cerebral cortex and cerebellum, identifying nine cell types, and integrated single-cell RNA sequencing data to explore cell type-specific regulatory landscapes and oligodendrocyte developmental trajectory. Furthermore, comparative analysis of each cell type between domestic pigs and wild boars indicated that oligodendrocyte progenitor cells may potentially exhibit a faster evolutionary rate. Finally, cross-species analysis suggested that, compared to humans, the proportion of sequence-conserved and functionally conserved regulatory elements in each cell type appears to be higher in pigs than in mice. Studies on the enrichment of genetic variants associated with 15 human diseases and complex traits in conserved regulatory elements across cell types indicated that immune-related diseases were more enriched in pigs, whereas neurological diseases were somewhat more enriched in mice. However, the enrichment of Alzheimer's disease-associated variants in pigs but not in mice suggests that pigs could be a more suitable model for this condition.</p><p><strong>Conclusions: </strong>Our research offers preliminary insights into the heterogeneity of pig brains and suggests the potential underlying regulatory mechanisms. Additionally, we explore the possible impact of nervous system differences on phenotypic changes, which could lay the groundwork for further biomedical studies.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"163"},"PeriodicalIF":4.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}