BMC Biology最新文献

{"title":"The phosphatase PPM1F, a negative regulator of integrin activity, is essential for embryonic development and controls tumor cell invasion.","authors":"Tanja M Grimm, Nina I Dierdorf, Marleen Herbinger, Sarah Baumgärtner, Erik Sontowski, Christoph Paone, Timo Baade, Christof R Hauck","doi":"10.1186/s12915-025-02254-3","DOIUrl":"10.1186/s12915-025-02254-3","url":null,"abstract":"<p><strong>Background: </strong>The Mn²⁺/Mg²⁺-dependent Ser/Thr phosphatase PPM1F was identified to control integrin activity. Furthermore, PPM1F regulates several protein kinases known to be involved in organizing the cytoskeleton and other cellular functions. Therefore, PPM1F appears critical for a multitude of physiological processes.</p><p><strong>Results: </strong>Here, we report the phenotype of ppm1f gene disruption in mice. While heterozygous ppm1f ± mice are viable and fertile, ppm1f-/- mice show severe defects and significant morphological abnormalities in the developing brain and vasculature and abort embryonic development at day E10.5. Isolated ppm1f-/- MEFs or PPM1F-depleted human neuro-epithelial cells display enhanced integrin-dependent cell adhesion, deregulated PAK phosphorylation, and perturbed cell migration. These phenotypes were reversed by re-expression of the wildtype enzyme, but not the phosphatase-inactive PPM1F. In different human tumor cell types, PPM1F expression levels directly correlated with invasive potential, while deletion of PPM1F abrogates tissue invasion.</p><p><strong>Conclusions: </strong>These results highlight the non-redundant role of this enzyme in integrin and PAK regulation and identify PPM1F as a promising target to limit tumor metastasis.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"166"},"PeriodicalIF":4.4,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12180154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Author Correction: Tyrosinase in melanoma inhibits anti-tumor activity of PD-1 deficient T cells.","authors":"Rong Huang, Yingbin Wang, Haitao Teng, Mengjun Xu, Kexin He, Yingzhuo Shen, Guo Guo, Xinyu Feng, Tianhan Li, Binhui Zhou, Marc Bajenoff, Toby Lawrence, Yinming Liang, Liaoxun Lu, Lichen Zhang","doi":"10.1186/s12915-025-02285-w","DOIUrl":"10.1186/s12915-025-02285-w","url":null,"abstract":"","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"165"},"PeriodicalIF":4.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12168257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond venomous fangs: Uloboridae spiders have lost their venom but not their toxicity.","authors":"Xiaojing Peng, Ludwig Dersch, Josephine Dresler, Tim Lüddecke, Tim Dederichs, Peter Michalik, Steve Peigneur, Jan Tytgat, Afrah Hassan, Antonio Mucciolo, Marc Robinson-Rechavi, Giulia Zancolli","doi":"10.1186/s12915-025-02248-1","DOIUrl":"10.1186/s12915-025-02248-1","url":null,"abstract":"<p><strong>Background: </strong>Venom, one of nature's most potent secretions, has played a crucial role in the evolutionary success of many animal groups, including spiders. However, Uloboridae spiders appear to lack venom and capture their prey, unlike venomous spiders, by extensive silk-wrapping and regurgitation of digestive fluids onto the entire prey package. A prevailing hypothesis posits that toxins may have been reallocated from the venom to alternative secretions, like silk or digestive fluids. Yet, whether uloborids have retained venom toxins and the mechanisms underlying prey immobilisation remain unresolved. Here, we employed a multi-disciplinary approach to assess the absence of venom glands in Uloborus plumipes, toxin gene expression and toxicity of digestive proteins.</p><p><strong>Results: </strong>Our findings confirm that U. plumipes lacks a venom apparatus, while neurotoxin-like transcripts were highly expressed in the digestive system. Midgut extract had comparable toxicity levels to that of the venomous Parasteatoda tepidariorum. However, no inhibitory effects on sodium nor potassium channels were observed, indicating a different toxic mechanism.</p><p><strong>Conclusions: </strong>These findings support the hypothesis that Uloboridae spiders have lost their venom apparatus while retaining toxin-like genes. The potent toxicity of their digestive fluids, a trait conserved across spiders, likely compensate for the absence of venom, ensuring effective prey immobilisation and digestion.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"159"},"PeriodicalIF":4.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12164160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell chromatin accessibility profiling reveals regulatory mechanisms and evolution in pig brains.","authors":"Yue Xiang, Saixian Zhang, Yi Huang, Zhuqing Zheng, Jiahui Sun, Qiulin Zhao, Peng Zhou, Xiaolong Qi, Jingjin Li, Fuyang Xiong, Jing Xu, Shengquan Wang, Liangliang Fu, Xinyun Li","doi":"10.1186/s12915-025-02263-2","DOIUrl":"10.1186/s12915-025-02263-2","url":null,"abstract":"<p><strong>Background: </strong>Pig brains serve as a valuable biomedical model for studying brain-related diseases due to their significant structural similarities to the human brain. Furthermore, the long-term domestication and artificial selection of domestic pigs have profoundly shaped their brains, making them an interesting subject for research. However, a comprehensive understanding of the regulatory mechanisms governing pig brain function and their impact on various phenotypes remains elusive due to the high degree of cellular heterogeneity present in the brain.</p><p><strong>Results: </strong>In this study, we profiled 71,798 cells from domestic pig and wild boar cerebral cortex and cerebellum, identifying nine cell types, and integrated single-cell RNA sequencing data to explore cell type-specific regulatory landscapes and oligodendrocyte developmental trajectory. Furthermore, comparative analysis of each cell type between domestic pigs and wild boars indicated that oligodendrocyte progenitor cells may potentially exhibit a faster evolutionary rate. Finally, cross-species analysis suggested that, compared to humans, the proportion of sequence-conserved and functionally conserved regulatory elements in each cell type appears to be higher in pigs than in mice. Studies on the enrichment of genetic variants associated with 15 human diseases and complex traits in conserved regulatory elements across cell types indicated that immune-related diseases were more enriched in pigs, whereas neurological diseases were somewhat more enriched in mice. However, the enrichment of Alzheimer's disease-associated variants in pigs but not in mice suggests that pigs could be a more suitable model for this condition.</p><p><strong>Conclusions: </strong>Our research offers preliminary insights into the heterogeneity of pig brains and suggests the potential underlying regulatory mechanisms. Additionally, we explore the possible impact of nervous system differences on phenotypic changes, which could lay the groundwork for further biomedical studies.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"163"},"PeriodicalIF":4.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential risk of dipterocarps in the marginal Asian rainforests: low population size and high genomic erosion.","authors":"Xian-Liang Zhu, Wen-Ji Luo, Sheng-Feng Chai, Jian-Min Tang, Xiao Wei, Alison K S Wee, Ming Kang","doi":"10.1186/s12915-025-02275-y","DOIUrl":"10.1186/s12915-025-02275-y","url":null,"abstract":"<p><strong>Background: </strong>Vatica guangxiensis, one of the northernmost dipterocarp tree species in Asian tropical rainforests and among the most critically endangered members of the Dipterocarpaceae, faces severe genetic risks due to its extremely small population size.</p><p><strong>Results: </strong>We present a chromosome-scale genome assembly of V. guangxiensis, totaling 454.89 Mb, comprising 54 scaffolds (contig N50 = 38.11 Mb). To inform conservation strategies, we conducted a population genomic analysis involving 148 individuals from three natural populations of V. guangxiensis sensu lato and two of V. mangachapoi, a widely distributed tropical rainforest species in Asia. Our results clarified the population structure, interspecific differentiation, and species delimitation of V. guangxiensis sensu lato, resolving longstanding taxonomic ambiguities and supporting the reinstatement of the two Yunnan populations as V. xishuangbannaensis. Compared with V. xishuangbannaensis and V. mangachapoi, V. guangxiensis exhibits significantly lower genetic diversity, higher inbreeding, and an elevated mutation load. Additionally, we revealed a near-complete absence of gene flow with related species and documented historically low effective population sizes, underscoring its vulnerability to genome erosion. Notably, genomic comparisons between mature and regenerating individuals revealed a 26.45% decrease in genetic diversity and a 10.70% increase in mutation load in smaller trees of V. guangxiensis, reflecting accelerated genomic erosion during natural regeneration.</p><p><strong>Conclusions: </strong>Our findings underscore the urgent need to monitor genomic health and implement effective conservation measures to safeguard V. guangxiensis. This study highlights the critical role of genomic data in assessing population health and informing strategies for the preservation of endangered species in marginal tropical rainforests.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"161"},"PeriodicalIF":4.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovarian transcriptome analyses indicate that weak juvenile hormone signaling underlies the molecular basis of oogenesis deficiencies in mosquitoes.","authors":"Monika M Wiśniewska, Martin Kolísko, Petra Berková, Martin Moos, Houda O Maaroufi, Fernando G Noriega, Marcela Nouzova","doi":"10.1186/s12915-025-02266-z","DOIUrl":"10.1186/s12915-025-02266-z","url":null,"abstract":"<p><strong>Background: </strong>Juvenile hormone (JH) is synthesized by the corpora allata (CA) and controls development and reproduction in insects. We recently used CRISPR/Cas9 to establish a line lacking the enzyme that catalyzes the final step of JH biosynthesis in mosquitoes, a P450 epoxidase. The CA of the epox^-/- mutants do not synthesize epoxidized JH III but methyl farneosate (MF), a weak agonist of the JH receptor. Female epox^-/- mosquitoes have reduced JH signaling and show a substantial loss of reproductive fitness. To understand the molecular basis of this loss of fitness, we constructed ovarian mRNA libraries of Ae. aegypti of the Orlando strain wild-type (WT) and epoxidase null mutants (epox^-/-) and investigated differential expression of reproductive genes.</p><p><strong>Results: </strong>We performed triplicate RNA-seq analyses of female WT and epox^-/- ovaries dissected at four critical stages of oogenesis: Ovaries from newly eclosed females (0h), sugar-fed females at 4 days post-eclosion (4d SF), females 16h (16h BF), and 48 h after a blood meal (48h BF). Silencing of epoxidase resulted in a drastic change in the expression of thousands of genes.</p><p><strong>Conclusions: </strong>Our results suggest that epoxidase deficiency leads to a reduction in JH signaling that has significant effects on Ae. aegypti ovarian transcriptome profiles. Ecdysteroid titers are dysregulated in the mutants, leading to a significant delay in the expression of vitelline membrane genes and other transcripts. We discovered changes in the expression of 230 long non-coding RNAs (lncRNAs) that may play an important role in the regulation of ovarian genes.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"160"},"PeriodicalIF":4.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering alternative splicing patterns during cell fate transition of fast chemical reprogramming.","authors":"Yunkun Lu, Kainan Lin, Yeling Ruan, Junjie Li, Huizhen Zhang, Tianyuan Pan, Qianqian Wang, Lianyu Lin, Sijie Feng","doi":"10.1186/s12915-025-02264-1","DOIUrl":"10.1186/s12915-025-02264-1","url":null,"abstract":"<p><strong>Background: </strong>Alternative splicing (AS) is a substantial contributor to the high complexity of transcriptomes in multicellular eukaryotes. Fast chemical reprogramming (FCR) system is an innovative approach that facilitates the rapid transition of somatic cells into induced pluripotent stem cells (iPSCs).</p><p><strong>Results: </strong>In this study, we used the FCR system to delve into the dynamics of AS during cell fate transition. The trajectory of FCR, as characterized by gene expression profiles, consistently aligned with that observed in AS patterns, revealing a complex interplay between AS and gene expression regulation. Additionally, we discovered that the exon exclusion events were more prevalent than the exon inclusion events, indicating a predominant mode of splicing regulation during FCR. Compared to transcription factor-induced reprogramming (TFR), FCR showed a distinct AS pattern, underscoring the unique regulatory mechanisms governing AS in each reprogramming system. Further investigation uncovered polypyrimidine tract-binding protein 3 (Ptbp3) as an important splicing factor, possibly participating in epigenetic regulation in late stage of FCR by affecting AS of epigenetic regulators. Moreover, we found an abundance of intron retention events caused by decrease in spliceosome activity, potentially contributing to the downregulation of key diapause-related genes in the middle and late stages of FCR.</p><p><strong>Conclusions: </strong>This research provided a comprehensive characterization of AS during FCR, highlighting the pivotal roles of AS in regulating cell fate transitions. Our findings advanced the understanding of the molecular mechanisms governing cell fate decisions and offered new insights into the potential of FCR for regenerative medicine and therapeutic applications.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"164"},"PeriodicalIF":4.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"iHofman: a predictive model integrating high-order and low-order features with weighted attention mechanisms for circRNA-miRNA interactions.","authors":"Chang-Qing Yu, Chen Jiang, Lei Wang, Zhu-Hong You, Xin-Fei Wang, Meng-Meng Wei, Tai-Long Shi, Si-Zhe Liang","doi":"10.1186/s12915-025-02260-5","DOIUrl":"10.1186/s12915-025-02260-5","url":null,"abstract":"<p><strong>Background: </strong>Increasing research indicates that the complex interactions between circular RNAs (circRNAs) and microRNAs (miRNAs) are critical for diagnosing and treating various human diseases. Consequently, accurately predicting potential circRNA-miRNA interactions (CMIs) has become increasingly important and urgent. Traditional biological experiments, however, are often labor-intensive, time-consuming, and prone to external influences.</p><p><strong>Results: </strong>To tackle this challenge, we present a novel model, iHofman, designed to predict CMIs by integrating high-order and low-order features with weighted attention mechanisms. Specifically, we first extract sequence and structural information representations using FastText and GraRep, respectively, and capture high-order and low-order features from sequence information representations using stacked autoencoders. Subsequently, weighted attention mechanisms are applied for feature fusion, focusing on the most relevant information. Finally, multi-layer perceptron is employed to accurately infer potential CMIs. In the fivefold cross-validation (CV) experiment on the baseline dataset, iHofman achieved an accuracy of 82.49% with an AUC of 0.9092. iHofman also demonstrates solid performance on other CMI datasets. In case studies, 26 of the top 30 CMIs with the highest iHofman predictive scores were confirmed in relevant literature.</p><p><strong>Conclusions: </strong>The above experimental results indicate that iHofman can effectively predict potential CMIs and has achieved outstanding performance compared with existing methods. It provides a reliable supplementary approach for subsequent biological wet experiments.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"162"},"PeriodicalIF":4.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferroptosis-related LncRNAs in diseases.","authors":"Wu Zhou, Jean Paul Thiery","doi":"10.1186/s12915-025-02268-x","DOIUrl":"10.1186/s12915-025-02268-x","url":null,"abstract":"<p><p>Ferroptosis is a form of regulated cell death (RCD) caused by the accumulation of intracellular iron and lipids and is involved in many pathological processes, including neurodegenerative and cardiovascular diseases, and cancer. Long non-coding RNAs (lncRNAs), RNA molecules exceeding 200 nt in length that do not possess protein coding function can interfere with ferroptosis by binding ferroptosis-related miRNAs or proteins. Recently, ferroptosis-related lncRNAs (FRlncRNAs) have been identified in cancer and non-malignant disease models, including inprediction of drug resistance, intra-tumoral immune infiltration, metabolic reprogramming and mutation landscape. Here, we review FRlncRNAs in cancer and non-malignant diseases, from prognosis to treatment.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"158"},"PeriodicalIF":4.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shrub encroachment enhances AMF network stability and complexity, while cropland destabilizes AMF communities in a subtropical alpine grassland.","authors":"Junqin Li, Yujun Zhang, Yang Gao, Xiangtao Wang, Yuting Yang, Denghui Wang, Lili Zhao, Puchang Wang","doi":"10.1186/s12915-025-02267-y","DOIUrl":"10.1186/s12915-025-02267-y","url":null,"abstract":"<p><strong>Background: </strong>Ecosystem conversion, primarily driven by agricultural expansion, has profoundly altered ecosystem structure and function. Grasslands, characterized by deep, nutrient-rich soils that support high soil carbon content, are particularly vulnerable to conversion for agricultural purposes. This transformation significantly impacts soil microbial communities, yet the effects of such changes on the stability and complexity of arbuscular mycorrhizal fungi (AMF) networks remain poorly understood, particularly in subtropical alpine grasslands.</p><p><strong>Results: </strong>In this study, we investigated how the conversion of natural grasslands into shrublands, artificial woodlands, and croplands affects AMF communities in a subtropical alpine region of China. Our results demonstrate that shrub encroachment increased AMF diversity by up to 25%, and enhanced network modularity and robustness by approximately 20% and 25%, respectively, compared with natural grasslands. This phenomenon may be partially attributed to deep root-mediated niche diversification and the alleviation of soil disturbance in shrubland. In contrast, conversion to cropland decreased AMF diversity by nearly 40%, destabilizing microbial networks due to increased nutrient enrichment and mechanical disturbance. Although the dominant genera Glomus and Paraglomus persisted across all systems, their relative abundance shifted (e.g., a 10-15% reduction of Glomus in croplands). Soil organic carbon, nitrogen, and phosphorus collectively explained up to 89.7% of the variation in AMF network complexity.</p><p><strong>Conclusions: </strong>These findings address the critical knowledge gap identified in the background regarding AMF responses to land-use changes in subtropical alpine grasslands. By demonstrating that shrub encroachment enhances soil fertility and AMF network stability-contrasting sharply with cropland conversion-our results highlight the importance of preserving natural succession processes to maintain microbial-driven ecosystem functions. This aligns with global efforts to mitigate grassland degradation and supports sustainable management practices in vulnerable alpine regions.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"157"},"PeriodicalIF":4.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}