BMC Biology最新文献

{"title":"Melanin deposition and key molecular features in Xenopus tropicalis oocytes.","authors":"Hongyang Yi, Weizheng Liang, Sumei Yang, Han Liu, Jiayu Deng, Shuhong Han, Xiaohui Feng, Wenjie Cheng, Yonglong Chen, Jing Hang, Hongzhou Lu, Rensen Ran","doi":"10.1186/s12915-025-02168-0","DOIUrl":"10.1186/s12915-025-02168-0","url":null,"abstract":"<p><strong>Background: </strong>Melanin pigmentation in oocytes is a critical feature for both the esthetic and developmental aspects of oocytes, influencing their polarity and overall development. Despite substantial knowledge of melanogenesis in melanocytes and retinal pigment epithelium cells, the molecular mechanisms underlying oocyte melanogenesis remain largely unknown.</p><p><strong>Results: </strong>Here, we compare the oocytes of wild-type, tyr^-/- and mitf^-/- Xenopus tropicalis and found that mitf^-/- oocytes exhibit normal melanin deposition at the animal pole, whereas tyr^-/- oocytes show no melanin deposition at this site. Transmission electron microscopy confirmed that melanogenesis in mitf^-/- oocytes proceeds normally, similar to wild-type oocytes. Transcriptomic analysis revealed that mitf^-/- oocytes still express melanogenesis-related genes, enabling them to complete melanogenesis. Additionally, in Xenopus tropicalis oocytes, the expression of the MiT subfamily factor tfe3 is relatively high, while tfeb, mitf, and tfec levels are extremely low. The expression pattern of tfe3 is similar to that of tyr and other melanogenesis-related genes. Thus, melanogenesis in Xenopus tropicalis oocytes is independent of Mitf and may be regulated by other MiT subfamily factors such as Tfe3, which control the expression of genes like tyr, dct, and tyrp1. Furthermore, transcriptomic data revealed that changes in the expression of genes related to mitochondrial cloud formation represent the most significant molecular changes during oocyte development.</p><p><strong>Conclusions: </strong>Overall, these findings suggest that further elucidation of Tyr-dependent and Mitf-independent mechanisms of melanin deposition at the animal pole will enhance our understanding of melanogenesis and Oogenesis.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"62"},"PeriodicalIF":4.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MultiCycPermea: accurate and interpretable prediction of cyclic peptide permeability using a multimodal image-sequence model.","authors":"Zixu Wang, Yangyang Chen, Yifan Shang, Xiulong Yang, Wenqiong Pan, Xiucai Ye, Tetsuya Sakurai, Xiangxiang Zeng","doi":"10.1186/s12915-025-02166-2","DOIUrl":"10.1186/s12915-025-02166-2","url":null,"abstract":"<p><strong>Background: </strong>Cyclic peptides, known for their high binding affinity and low toxicity, show potential as innovative drugs for targeting \"undruggable\" proteins. However, their therapeutic efficacy is often hindered by poor membrane permeability. Over the past decade, the FDA has approved an average of one macrocyclic peptide drug per year, with romidepsin being the only one targeting an intracellular site. Biological experiments to measure permeability are time-consuming and labor-intensive. Rapid assessment of cyclic peptide permeability is crucial for their development.</p><p><strong>Results: </strong>In this work, we proposed a novel deep learning model, dubbed as MultiCycPermea, for predicting cyclic peptide permeability. MultiCycPermea extracts features from both the image information (2D structural information) and sequence information (1D structural information) of cyclic peptides. Additionally, we proposed a substructure-constrained feature alignment module to align the two types of features. MultiCycPermea has made a leap in predictive accuracy. In the in-distribution setting of the CycPeptMPDB dataset, MultiCycPermea reduced the mean squared error (MSE) by approximately 44.83% compared to the latest model Multi_CycGT (0.29 vs 0.16). By leveraging visual analysis tools, MultiCycPermea can reveal the relationship between peptide modification structures and membrane permeability, providing insights to improve the membrane permeability of cyclic peptides.</p><p><strong>Conclusions: </strong>MultiCycPermea provides an effective tool that accurately predicts the permeability of cyclic peptides, offering valuable insights for improving the membrane permeability of cyclic peptides. This work paves a new path for the application of artificial intelligence in assisting the design of membrane-permeable cyclic peptides.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"63"},"PeriodicalIF":4.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical stimulation in plants: molecular insights, morphological adaptations, and agricultural applications in monocots.","authors":"Annalene Hansen, Agnieszka Gladala-Kostarz, Rebecca Hindhaugh, John H Doonan, Maurice Bosch","doi":"10.1186/s12915-025-02157-3","DOIUrl":"10.1186/s12915-025-02157-3","url":null,"abstract":"<p><p>Mechanical stimulation, including wind exposure, is a common environmental factor for plants and can significantly impact plant phenotype, development, and growth. Most responses to external mechanical stimulation are defined by the term thigmomorphogenesis. While these morphogenetic changes in growth and development may not be immediately apparent, their end-results can be substantial. Although mostly studied in dicotyledonous plants, recently monocot grasses, particularly cereal crops, have received more attention. This review summarizes current knowledge on mechanical stimulation in plants, particularly focusing on the molecular, physiological, and phenological responses in cereals, and explores practical applications to sustainably improve the resilience of agricultural crops.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"58"},"PeriodicalIF":4.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11863685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Receptor deorphanization in starfish reveals the evolution of relaxin signaling as a regulator of reproduction.","authors":"Yuling Feng, Nayeli Escudero Castelán, Mohammed Akhter Hossain, Hongkang Wu, Hidekazu Katayama, Stuart J Smith, Scott F Cummins, Masatoshi Mita, Ross A D Bathgate, Maurice R Elphick","doi":"10.1186/s12915-025-02158-2","DOIUrl":"10.1186/s12915-025-02158-2","url":null,"abstract":"<p><strong>Background: </strong>Relaxins are a family of peptides that regulate reproductive physiology in vertebrates. Evidence that this is an evolutionarily ancient role of relaxins has been provided by the discovery of two relaxin-like gonad-stimulating peptides (RGP1 and RGP2) that trigger spawning in starfish. The main aim of this study was to identify the receptor(s) that mediate(s) the effects of RGP1 and RGP2 in starfish.</p><p><strong>Results: </strong>Here we show that RGP1 and RGP2 belong to a family of peptides that include vertebrate relaxins, Drosophila insulin-like peptide 8 (Dilp8), and other relaxin-like peptides in several protostome taxa. An ortholog of the human relaxin receptors RXFP1 and RXFP2 and the Drosophila receptor LGR3 was identified in starfish (RXFP/LGR3). In Drosophila, but not in humans and other vertebrates, there is a paralog of LGR3 known as LGR4, and here an LGR4-type receptor was also identified in starfish. In vitro pharmacological experiments revealed that both RGP1 and RGP2 act as ligands for RXFP/LGR3 in the starfish Acanthaster cf. solaris and Asterias rubens, but neither peptide acts as a ligand for LGR4 in these species.</p><p><strong>Conclusions: </strong>Discovery of the RXFP/LGR3-type receptor for RGP1 and RGP2 in starfish provides a new insight into the evolution of relaxin-type signaling as a regulator of reproductive processes. Furthermore, our findings indicate that RXFP/LGR3-type receptors have been lost in several phyla, including urochordates, mollusks, bryozoans, platyhelminthes, and nematodes.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"59"},"PeriodicalIF":4.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11863921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing ferroptosis for precision oncology: challenges and prospects.","authors":"Roberto Fernández-Acosta, Iuliana Vintea, Ine Koeken, Behrouz Hassannia, Tom Vanden Berghe","doi":"10.1186/s12915-025-02154-6","DOIUrl":"10.1186/s12915-025-02154-6","url":null,"abstract":"<p><p>The discovery of diverse molecular mechanisms of regulated cell death has opened new avenues for cancer therapy. Ferroptosis, a unique form of cell death driven by iron-catalyzed peroxidation of membrane phospholipids, holds particular promise for targeting resistant cancer types. This review critically examines current literature on ferroptosis, focusing on its defining features and therapeutic potential. We discuss how molecular profiling of tumors and liquid biopsies can generate extensive multi-omics datasets, which can be leveraged through machine learning-based analytical approaches for patient stratification. Addressing these challenges is essential for advancing the clinical integration of ferroptosis-driven treatments in cancer care.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"57"},"PeriodicalIF":4.4,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11849278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the thermotolerance mechanism of Pichia kudriavzevii LC375240 through transcriptomic and genetic analyses.","authors":"Yanhua Qi, Qijian Qin, Jiayin Ma, Bin Wang, Cheng Jin, Wenxia Fang","doi":"10.1186/s12915-025-02159-1","DOIUrl":"10.1186/s12915-025-02159-1","url":null,"abstract":"<p><strong>Background: </strong>Thermotolerance is a critical trait for yeasts employed in industrial settings, and the utilization of unconventional yeasts has gained notable attention in recent years. However, the mechanisms underlying thermotolerance in unconventional yeasts, particularly Pichia spp., remain insufficiently elucidated.</p><p><strong>Results: </strong>This study focuses on the thermotolerance of a non-traditional yeast strain Pichia kudriavzevii LC375240, renowned for its remarkable thermotolerance. Through transcriptomic analysis of both short-term and long-term heat shock exposures, we uncovered an intricate regulatory response in P. kudriavzevii. During long-term heat treatment, the yeast exhibited elevated expression of genes involved in the tricarboxylic acid (TCA) cycle and suppressed expression of genes in the pentose phosphate pathway (PPP). Additionally, long-term heat treatment led to an upregulation of heat shock proteins (HSPs) and an increase in trehalose, glutathione (GSH), and superoxide dismutase (SOD) levels, along with a reduction in the intracellular NADPH/NADP⁺ ratio and pyruvate content. These changes collectively contribute to the thermotolerance of P. kudriavzevii. CRISPR-Cas9-mediated knockout experiments further highlighted the critical roles of HSPs, antioxidases, and the trehalose metabolic pathway in the yeast's response to high temperatures.</p><p><strong>Conclusions: </strong>Taken together, this study demonstrates that P. kudriavzevii adapts to thermal stress through a combination of enhanced TCA cycle, reduced PPP, increased HSPs, trehalose, GSH, and SOD levels. These findings provide a comprehensive understanding of the molecular mechanisms underlying thermotolerance in P. kudriavzevii.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"55"},"PeriodicalIF":4.4,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11849260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of a pheromone binding protein affected by timeless gene governs female mating behavior in Bactrocera dorsalis.","authors":"Yuting Jiao, Guohong Luo, Yongyue Lu, Daifeng Cheng","doi":"10.1186/s12915-025-02164-4","DOIUrl":"10.1186/s12915-025-02164-4","url":null,"abstract":"<p><strong>Background: </strong>The rhythmic mating behavior of insects has been extensively documented, yet the regulation of this behavior through sex pheromone sensing olfactory genes affected by the clock genes in the rhythm pathway remains unclear.</p><p><strong>Results: </strong>In this study, we investigated the impact of circadian rhythm on female recognition of male rectal Bacillus-produced sex pheromone in B. dorsolis. Behavioral and electrophysiological assays revealed a peak in both mating behavior and response to sex pheromones in the evening in females. Comparative transcriptome analysis of female heads demonstrated rhythmic expression of the Timeless gene-Tim and odorant binding protein gene-Pbp5, with the highest expression levels occurring in the evening. Protein structural modeling, tissue expression patterns, RNAi treatment, and physiological/behavioral studies supported Pbp5 as a sex pheromone binding protein whose expression is affected by Tim. Furthermore, manipulation of the female circadian rhythm resulted in increased morning mating activity, accompanied by consistent peak expression of Tim and Pbp5 during this time period. These findings provide evidence that insect mating behavior can be modulated by clock genes through their effects on sex pheromone sensing processes.</p><p><strong>Conclusions: </strong>Our results also contribute to a better understanding of the molecular mechanisms underlying rhythmic insect mating behavior.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"56"},"PeriodicalIF":4.4,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11849186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The origin, evolution, and translocation of sex chromosomes in Silurus catfish mediated by transposons.","authors":"Shuqing Zheng, Hongyan Tao, Yuheng Song, Mao Li, Haowen Yang, Jianzhen Li, Hongwei Yan, Bakhtiyor Sheraliev, Wenjing Tao, Zuogang Peng, Yaoguang Zhang, Deshou Wang","doi":"10.1186/s12915-025-02160-8","DOIUrl":"10.1186/s12915-025-02160-8","url":null,"abstract":"<p><strong>Background: </strong>Sex chromosome (SC) evolution is a longstanding topic of focus in evolutionary biology. Teleosts often exhibit rapid turnover of SCs and sex-determining (SD) genes, alongside a diverse range of SC differentiation mechanisms.</p><p><strong>Results: </strong>On the basis of new chromosome-scale assemblies of three Silurus species (S. microdorsalis, S. glanis, and S. lanzhouensis) and two outgroup species (Pterocryptis cochinchinensis and Kryptopterus bicirrhis), along with our previous assemblies of S. meridionalis and S. asotus, we traced the evolution of SC in the Silurus genus (Siluriformes), following the fate of the known SD gene amhr2y. Phylogenetic analysis showed that amhr2y occurred at least before the divergence of Pterocryptis, Kryptopterus, and Silurus and lost in P. cochinchinensis and K. bicirrhis. Chr24 has become the SC in the ancestor of five Silurus species due to the duplication-and-translocation of amhr2 mediated by LTR transposon. Then, a proto Y was formed and maintained with a shared 60 kb male-specific region of the Y chromosome (MSY) by transposable elements (TEs) expansion and gene gathering. Due to the continuous TEs accumulation, genes other than amhr2y in MSYs have degenerated or been lost, while non-recombinant regions continue to expend, forming MSYs of different sizes in different Silurus species (from 320 to 550 kb). Two turnover events, one homologous (from the left arm to the right arm of Chr24) and one nonhomologous (from Chr24 to Chr5), occurring among five Silurus species were possibly mediated by hAT and Helitron transposons.</p><p><strong>Conclusions: </strong>Our results on the dynamic evolutionary trajectory of SD gene amhr2y, MSYs, and SCs in Silurus catfish indicated the variability and diversity of fish SCs and confirmed that frequent turnover is an important way to maintain the homology and low differentiation of fish SCs.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"54"},"PeriodicalIF":4.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A whole-body atlas of BMP signaling activity in an adult sea anemone.","authors":"Paul Knabl, David Mörsdorf, Grigory Genikhovich","doi":"10.1186/s12915-025-02150-w","DOIUrl":"10.1186/s12915-025-02150-w","url":null,"abstract":"<p><strong>Background: </strong>BMP signaling is responsible for the second body axis patterning in Bilateria and in the bilaterally symmetric members of the bilaterian sister clade Cnidaria-corals and sea anemones. However, medusozoan cnidarians (jellyfish, hydroids) are radially symmetric, and yet their genomes contain BMP signaling components. This evolutionary conservation suggests that BMP signaling must have other functions not related to axial patterning, which keeps BMP signaling components under selective pressure.</p><p><strong>Results: </strong>To find out what these functions might be, we generated a detailed whole-body atlas of BMP activity in the sea anemone Nematostella. In the adult polyp, we discover an unexpected diversity of domains with BMP signaling activity, which is especially prominent in the head, as well as across the neuro-muscular and reproductive parts of the gastrodermis. In accordance, analysis of two medusozoan species, the true jellyfish Aurelia and the box jellyfish Tripedalia, revealed similarly broad and diverse BMP activity.</p><p><strong>Conclusions: </strong>Our study reveals multiple, distinct domains of BMP signaling in Anthozoa and Medusozoa, supporting the versatile nature of the BMP pathway across Cnidaria. Most prominently, BMP signaling appears to be involved in tentacle formation, neuronal development, and gameto- or gonadogenesis.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"49"},"PeriodicalIF":4.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization and evolution of olfactory responses in coeloconic sensilla of the global fruit pest Drosophila suzukii.","authors":"Qi Xue, Kazi Sifat Hasan, Omar Dweck, Shimaa A M Ebrahim, Hany K M Dweck","doi":"10.1186/s12915-025-02151-9","DOIUrl":"10.1186/s12915-025-02151-9","url":null,"abstract":"<p><strong>Background: </strong>When a species changes its host preference, it often requires modifications in its sensory systems. Many of these changes remain largely uninvestigated in the global fruit pest Drosophila suzukii (also known as spotted wing Drosophila, SWD). This species, which shares a last common ancestor with the model organism D. melanogaster-a species that prefers overripe fruits- ~ 15 million years ago, has shifted its preference from overripe to ripe, soft-skinned fruits, causing significant damage to fruit industries worldwide.</p><p><strong>Results: </strong>Here, we functionally characterized the coeloconic sensilla in D. suzukii and compared their responses to those of its close relatives, D. biarmipes and D. melanogaster. We find that D. suzukii's responses are grouped into four functional types. These responses are consistent across sexes and reproductive status. The odorant receptor co-receptor Orco is required for certain responses. Comparative analysis across these species revealed evolutionary changes in physiological and behavioral responses to specific odorants, such as acetic acid, a key indicator of microbial fermentation, and phenylacetaldehyde, an aromatic compound found in a diverse range of fruits. Phenylacetaldehyde produced lower electrophysiological responses in D. suzukii compared to D. melanogaster and elicited strong attraction in D. suzukii but not in any of the other tested species.</p><p><strong>Conclusions: </strong>The olfactory changes identified in this study likely play a significant role in the novel behavior of D. suzukii. This work also identifies phenylacetaldehyde as a potent attractant for D. suzukii, which can be used to develop targeted management strategies to mitigate the serious impact of this pest.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"50"},"PeriodicalIF":4.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}