BMC Biology最新文献

{"title":"Multi-omics analysis reveals diapause-associated lipid remodeling in the fat body of Colorado potato beetle.","authors":"Doga Cedden, Gözde Güney, Şerife Bayram, Doug Baldwin, Cathy Coutu, Dwayne Hegedus, Megan N Lipke, Cansu Doğan, Russell L Groves, Umut Toprak","doi":"10.1186/s12915-025-02408-3","DOIUrl":"10.1186/s12915-025-02408-3","url":null,"abstract":"<p><strong>Background: </strong>The Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae), is a major pest of potato that undergoes diapause (hibernation) to survive harsh winter conditions. Lipid metabolism plays a crucial role in diapause preparation and maintenance. However, the specific changes in lipid composition and their molecular regulation remain unclear. This study integrates lipidomics, transcriptomics, and proteomics to investigate diapause-associated metabolic shifts in the fat body of L. decemlineata.</p><p><strong>Results: </strong>We identified significant increases in monounsaturated (e.g., oleic acid) and polyunsaturated (e.g., linoleic acid) fatty acids, as well as phosphatidylethanolamines (membrane lipids) enriched in unsaturated fatty acids during diapause. Transcriptomic analysis revealed the differential expression of genes related to energy metabolism and lipid processing, while qualitative proteomics identified some calcium-associated protein isoforms (regucalcin-like and annexin B9-like) that are uniquely present in diapause. Correlation analysis suggested that specific transcripts, such as carboxyl ester lipase, are associated with the regulation of key lipid molecules, including oleic acid and linoleic acid, during diapause. These findings advance our understanding of lipid regulation during diapause and highlight potential molecular targets for disrupting overwintering strategies, which could inform novel pest control approaches.</p><p><strong>Conclusions: </strong>Diapause in the Colorado potato beetle is associated with changes in lipid composition in the fat body, which is mediated through changes at the transcriptome and proteome levels.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"294"},"PeriodicalIF":4.5,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the origins of guanylate-binding proteins in mammals (Monotreme, Marsupials and Placentals).","authors":"João Vasco Côrte-Real, Ana Pinheiro, Jordan M Sampson, Kimberly A Morrissey, João Pedro Marques, Hanna-Mari Baldauf, Robert D Miller, Joana Abrantes, Pedro José Esteves","doi":"10.1186/s12915-025-02403-8","DOIUrl":"10.1186/s12915-025-02403-8","url":null,"abstract":"<p><strong>Background: </strong>Guanylate-binding proteins (GBPs) belong to the large guanosine triphosphatases (GTPases) family and have specialised in host defence in vivo against a broad spectrum of invading pathogens. This ancient evolutionary group of genes was first studied in humans and rodents, but its evolution remained largely unknown for nearly 20 years. In recent years, more studies have emerged deepening the knowledge of GBP evolution in specific mammalian groups: Primates, Tupaia, Muroids (Rodents), Bats and Lagomorphs.</p><p><strong>Results: </strong>Here, we aimed to present a comprehensive analysis of mammals GBP evolution. Our phylogenetic analysis demonstrates that mammals' GBPs share a common ancestor and that each major mammalian group has evolved a specific GBP repertoire. Two Monotreme GBP groups, GBP8 and GBP9, cluster independently in the phylogenetic tree and do not share the synteny of the other mammalian GBP genes. The other two Monotreme GBP groups, GBP1/2/3/5 and GBP4/6/7, are at the basal position of the main mammalian groups. Marsupials have two GBP groups, Marsupial GBP1/2/3/5, basal to Placental GBP1/2/3/5, and Marsupial GBP4/6/7, basal to Placental GBP4/6/7. Marsupial GBP1/2/3/5 can be subdivided into three sub-groups, similarly to what is observed in the Placental GBPs, whereas Marsupial GBP4/6/7 underwent several duplication events across species. We also examined the GBP tissue expression pattern in Monodelphis domestica and found that GBPs are ubiquitously expressed in most tissues, with some differences. Noteworthy was the presence of GBP transcripts in late foetal and newborn opossum tissues.</p><p><strong>Conclusions: </strong>The GBP genes revealed a distinct evolutionary pattern in each main mammalian group. Phylogenetic analysis shows that Monotremes and Marsupials have specific GBPs. Particularly intriguing is the presence of GBP8 and GBP9 only in Monotremes.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"292"},"PeriodicalIF":4.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural variant landscape provides insights into genome organisation and domestication in European seabass.","authors":"Zexin Jiao, Robert Mukiibi, Manu Kumar Gundappa, Massimiliano Babbucci, James G D Prendergast, Diego Robledo, Luca Bargelloni, Daniel J Macqueen","doi":"10.1186/s12915-025-02404-7","DOIUrl":"10.1186/s12915-025-02404-7","url":null,"abstract":"<p><strong>Background: </strong>Structural variants (SVs) are genetic polymorphisms including deletions, insertions, inversions, and duplications, with potential to influence traits through impacts on gene function and expression. SVs have not been widely utilized in genetic analysis, owing to the challenge of their accurate detection and genotyping. Addressing this general issue, and the broader demand to understand their role in commercially important taxa, we report a comprehensive analysis of SVs in the genome of European seabass (Dicentrarchus labrax), the most commercially important fish in the Mediterranean region.</p><p><strong>Results: </strong>Using whole genome sequencing from a farmed population (n = 90 samples), 21,428 SVs were identified using a comprehensive detection and genotyping strategy involving manual curation of every variant. This high-confidence SV atlas was annotated to predict impacts on genes and evolutionarily conserved sequences. We explored the overlap between SVs and repeats, identified heterogeneity in SV density across the genome, and tested if the coding genes disrupted by SVs are enriched for specific biological processes or conserved protein domains. SVs impacting evolutionarily conserved genomic regions were enriched in genes with nervous system and developmental functions. Finally, we performed a comparative analysis incorporating 38,408 high-confidence SVs identified independently for three wild populations (n = 80 samples) using identical methods. An analysis of 41,336 SVs merged across the two datasets provides insights into genes and biological functions targeted during aquaculture domestication, with evidence of shifts in allele frequency for SVs located within or near genes controlling behaviour, enriched for forebrain and synaptic functions, and specifically expressed in the brain.</p><p><strong>Conclusions: </strong>This study sheds light on the global organisation of SVs across the European seabass genome, revealing a potential role in aquaculture domestication. The reported datasets provide a novel, high-quality reference for future genetic investigations of both farmed and wild European seabass.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"288"},"PeriodicalIF":4.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A W chromosome-derived feminizing piRNA in pyralid moths demonstrates convergent evolution for primary sex determination signals in Lepidoptera.","authors":"Sander Visser, Atsuo Yoshido, Irena Provazníková, Martina Dalíková, Dagmar Voříšková, Anna Chung Voleníková, Eveline C Verhulst, František Marec","doi":"10.1186/s12915-025-02392-8","DOIUrl":"10.1186/s12915-025-02392-8","url":null,"abstract":"<p><strong>Background: </strong>The primary signals of sex determination in insects are diverse and evolve erratically. This also appears to be the case with moths and butterflies. In the silkworm Bombyx mori, female development is triggered by a W chromosome-derived Feminizer (Fem) piRNA that degrades the mRNA of the Z-linked Masculinizer (Masc) gene, which controls male development. We investigated whether this mechanism is conserved in another group of Lepidoptera.</p><p><strong>Results: </strong>We identified a putative feminizing piRNA and many partial copies of the EkMasc gene on the W chromosome of Ephestia kuehniella. The piRNA is generated by a repetitive W-linked sequence named E. kuehniella Moth-overruler-of-masculinization (EkMom). EkMom piRNA shows high similarity to a region of Z-linked EkMasc and is expressed at the onset of female development, but has no relationship to the B. mori Fem piRNA. We then mapped small RNA-seq data from embryos of the related Plodia interpunctella to the PiMasc gene and identified a single small RNA, a PiMom piRNA, able to target PiMasc and with high sequence identity to the EkMom piRNA. Both the PiMom and EkMom repeats are present in high copy number and form a single cluster on the W chromosome. In both species, the Mom piRNA is responsible for Masc mRNA cleavage, clearly demonstrating that the Mom piRNA triggers female development.</p><p><strong>Conclusions: </strong>Our study provides multiple lines of evidence that Mom piRNA is the primary sex-determining signal in two pyralid moths and highlights a possible pathway for the origin of feminizing piRNAs in Lepidoptera. The similarity in female sex determination between the phylogenetically distant species suggests convergent evolution of feminizing piRNAs in Lepidoptera.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"289"},"PeriodicalIF":4.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complex competition interactions between Egyptian fruit bats and black rats in the real world.","authors":"Xing Chen, Lee Harten, Adi Rachum, Liraz Attia, Yossi Yovel","doi":"10.1186/s12915-025-02380-y","DOIUrl":"10.1186/s12915-025-02380-y","url":null,"abstract":"<p><strong>Background: </strong>Interspecific interactions, including competition and predation, are key drivers of ecological systems. Understanding these interactions remains challenging in the wild as it requires quantifying their effects, particularly the non-consumptive effects (NCEs) driven by predation risk. We conducted a 7-month study in a semi-natural open bat colony, monitoring interactions between Egyptian fruit bats (Rousettus aegyptiacus) and black rats (Rattus rattus) competing for food, where rats also pose a potential predation risk to the bats.</p><p><strong>Results: </strong>Video analysis revealed that bat responses to rats were fundamentally different from responses to conspecifics. The primary response was avoidance, with bat landings near food decreasing significantly when rats were present. For the 789 landings that did occur, bats showed increased vigilance and reduced foraging success, demonstrating clear NCEs. Crucially, bat foraging strategies were highly context-dependent, shifting with seasonal resource availability and rat abundance. During winter when rats were uncommon, the bats primarily employed predation risk-averse strategies (avoidance and vigilance). In spring, when rats were frequent, although there was clear temporal partitioning between the bat and the rat populations, some of the bats shifted to heterospecific interference competition, and occasionally attacked the rats to gain access to food-a behavior inconsistent with simple risk-aversion models.</p><p><strong>Conclusions: </strong>Our findings demonstrate that the bat-rat interactions are dynamically modulated by resource availability, which alters rat presence and thereby the context-dependent interplay between interference competition and NCEs. This study provides rare quantitative evidence of how behaviorally flexible animals strategically manage interference competition and predation risk based on seasonal ecological conditions.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"290"},"PeriodicalIF":4.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated 2D framework for quantifying cellular mechanics reveals the impact of juxtacrine Notch signalling on directed collective migration of endothelial cells.","authors":"Janine Grolleman, Carlijn V C Bouten, Vito Conte, Cecilia M Sahlgren","doi":"10.1186/s12915-025-02396-4","DOIUrl":"10.1186/s12915-025-02396-4","url":null,"abstract":"<p><strong>Background: </strong>Collective migration is the coordinated movement of a group of cells-a fundamental process in health and disease. Many models have been developed to study the molecular and physical mechanisms of collective migration. However, the aim of this study is to engineer a flexible in vitro framework that allows for mechanobiological quantification of the separate and combined contributions of individual cell mechanics to the directed migration of a collective. We utilised this framework to understand the role of juxtacrine Notch signalling during collective endothelial migration-an essential process during the formation of new blood vessels (known as angiogenesis).</p><p><strong>Results: </strong>This framework enables users to perform high spatiotemporal analysis of migrative behaviour, cell-matrix traction forces, and intercellular forces in different microenvironments. With this framework, we show that Notch inhibited collectives adopt a distinct regime of directed collective migration. Whereas the directionality of migration, traction forces and intercellular forces are not affected by Notch inhibition, we observed spatiotemporal differences in migration speed, traction force magnitude and normal and shear stresses within Notch-inhibited collectives.</p><p><strong>Conclusions: </strong>The in vitro framework is a powerful approach for dissecting the mechanisms of collective migration. With this framework, we show that a potential link exists between the juxtacrine signalling of Notch and an increased mechanical cohesiveness among collective cells.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"291"},"PeriodicalIF":4.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conservation genomics of two endangered buntings reveal genetic diversity before and after severe population declines.","authors":"Shangyu Wang, Dezhi Zhang, Xiaolu Jiao, Lei Wu, Qiang-Hui Zhu, Hongrui Lv, Haitao Wang, Zheng Han, Shi Li, Peng He, Jun Chen, Shaohong Feng, Urban Olsson, Per Alström, Fumin Lei","doi":"10.1186/s12915-025-02393-7","DOIUrl":"10.1186/s12915-025-02393-7","url":null,"abstract":"<p><strong>Background: </strong>Museomics utilizes historical genetic data from museum specimens to inform threatened species conservation. The Yellow-breasted Bunting (Emberiza aureola) and the Jankowski's Bunting (E. jankowskii), categorized as Critically Endangered or Endangered, respectively, have experienced population declines since the 1970s-1980s. Comparing genetic diversity changes before and after declines is crucial for refining conservation strategies.</p><p><strong>Results: </strong>We de novo assembled genomes for both species and resequenced 29 E. aureola (16 historical pre-decline specimens from the 1930s to the 1950s) and 18 E. jankowskii (4 historical pre-decline specimens from the 1950s to the 1960s), with 45 individuals from six least-concern Emberiza species for comparison. Genetic diversity remained stable in both endangered species from the pre-decline to post-decline periods, with their overall genetic diversity levels being comparable to those of their least-concern congeners. While historically, both had large effective population sizes, E. jankowskii showed a gradual decline over 1000 generations, whereas E. aureola remained stable. Both modern populations of E. aureola and E. jankowskii exhibited a higher proportion of long runs of homozygosity (ROH) compared to their historical counterparts, indicating an increased impact of inbreeding following population declines.</p><p><strong>Conclusions: </strong>Despite severe population declines, both species retained high genetic diversity but experienced increased inbreeding. E. jankowskii faces ongoing effective population size decline. These insights guide targeted conservation strategies, highlighting the value of museomics in understanding demographic and genetic histories.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"287"},"PeriodicalIF":4.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modification of adipose mass by targeting distal enhancers of Ptrf.","authors":"Yu Zhang, Keren Long, Songling Zhang, Biwei Zhang, Xiaokai Li, Xuemin Li, Zhining Zhong, Ziqi Li, Qiuyue Chen, Penghao Li, Li Chen, Jinwei Zhang, Jianlin Yuan, Anan Jiang, Lu Lu, Haohuan Li, Long Jin, Liangpeng Ge, Peng Shang, Xuewei Li, Mingzhou Li","doi":"10.1186/s12915-025-02405-6","DOIUrl":"10.1186/s12915-025-02405-6","url":null,"abstract":"<p><strong>Background: </strong>Adipose tissue plays a central role in systemically metabolic regulation. Polymerase I and transcript release factor (PTRF) is responsible for caveolae structure formation and plays a crucial role in lipid metabolism.</p><p><strong>Results: </strong>We investigated the possibility of modifying adipose mass by targeting the enhancers of Ptrf. Single-nucleus transcriptomic analysis revealed that the reduction in fat mass caused by Ptrf knockout is associated with impaired adipogenesis and differentiation, adipocyte metabolism, nutrient transportation, and altered regulatory network. We demonstrated a closely positive association between Ptrf transcription and adipose mass in mice. Then, circular chromosome conformation capture sequencing and ChIP-seq data of mature adipocytes identified six candidate enhancers (E1-E6) of Ptrf, and the dual-luciferase reporting system revealed the transcriptional activity of three active candidate enhancers (E1, E3, E5) in Human embryonic kidney 293 (HEK293) and mouse embryo fibroblast (3T3-L1) cells. During the adipogenic differentiation of 3T3-L1, the epigenetic perturbation of these enhancers with the dCas9-KRAB system demonstrated their important roles during adipogenesis, with inhibited Ptrf expression and decreased lipid droplets and triglyceride contents. Injecting lentivirus carrying the dCas9-KRAB system into the inguinal fat of mice downregulated Ptrf expression and decreased body weight, body adipose percentage, and adipocyte diameter in vivo. Additionally, we observed the close binding of Pparg and Cebpa to Ptrf-E1.</p><p><strong>Conclusions: </strong>Taken together, these findings identify the mechanism by which Ptrf deletion leads to reduced fat mass in mice, and indicate that adipose mass may be reduced by targeting cis-regulatory elements of key adipogenesis-related genes.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"286"},"PeriodicalIF":4.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A LXG toxin stabilized by DUF4176 contributes to Streptococcus suis competition and pathogenicity.","authors":"Huizhen Wu, Yifan Wu, Qiankun Bai, Zijing Liang, Xinchi Zhu, Xinming Pan, Minghui Liu, Huochun Yao, Jiale Ma, Zongfu Wu","doi":"10.1186/s12915-025-02391-9","DOIUrl":"10.1186/s12915-025-02391-9","url":null,"abstract":"<p><strong>Background: </strong>Streptococcus suis poses a significant threat to both humans and pigs, causing severe disease such as meningitis, septicaemia, and sudden death. The type VII secretion system (T7SS) plays a crucial role in exporting effectors that mediate bacterial competition and colonization in polymicrobial environments. Although Leu-x-Gly (LXG) polymorphic toxins are among the main effectors secreted by the T7SS, the mechanism underlying their secretion remains to be fully elucidated.</p><p><strong>Results: </strong>Here, we identified a complex repertoire of LXG effectors in S. suis, comprising at least five distinct LXG-domain groups and 14 C-terminal toxin types. We focused on one LXG effector, LXG1-CT3, from the virulent strain WUSS351, demonstrating its pore-forming activity and essential role in S. suis competition and virulence. Additionally, we discovered that a small protein family, DUF4176, partners with each cognate C-terminal toxin, stabilizing the corresponding LXG effector prior to secretion, thereby enhancing bacterial competitiveness.</p><p><strong>Conclusions: </strong>These findings deepen our understanding of S. suis competition and pathogenesis, while offering new insights into the T7SS-mediated secretion of LXG effectors.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"284"},"PeriodicalIF":4.5,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The high-quality genome of Bellamya aeruginosa reveals the molecular mechanisms underlying its remarkable stress resistance.","authors":"Gang Wang, Lianfu Chen, Haoran Pan, Aobo Pang, Yanxia Shi, Sisi Chen, Zhijuan Bian, Chijie Yin, Rongchen Liu, Xiaoli Sun, Xiaoxiao Wu, Sheng Tang, Fujun Xuan, Qiuning Liu, Shouquan Hou, Boping Tang, Daizhen Zhang","doi":"10.1186/s12915-025-02401-w","DOIUrl":"10.1186/s12915-025-02401-w","url":null,"abstract":"<p><strong>Background: </strong>Bellamya aeruginosa belongs to the family Viviparidae and is widely distributed in freshwater ecosystems in Asia. This species is resilient to thermal stress and can survive in polluted waters and consequently has been widely studied as a sentinel species for assessing ecosystem risk. To gain resources to study how B. aeruginosa adapts to harsh environments at the molecular level, we assembled a high-quality genome of this species and compared responses in transcriptome sequencing following exposure to wastewater, heat stress, and cold stress.</p><p><strong>Results: </strong>We assembled a pseudo-chromosome genome for B. aeruginosa (1.2 Gb, 8 chromosomes) through the integration of multiple sequencing strategies. Comparative genomic analyses demonstrated that genes for the expansion of the Viviparidae branch comprising Cipangopaludina cathayensis, Bellamya purificata, and B. aeruginosa were enriched in GO terms such as developmental growth involved in morphogenesis, regulation of animal organ morphogenesis, and regulation of embryonic development. This may be related to the pattern of viviparous reproduction in viviparids. Transcriptomic analyses revealed that this organism relies on lipid metabolism regulation and antioxidant systems to adapt to hypoxic stresses imposed by wastewater. Additionally, we identified multiple genes encoding heat shock proteins (Hsps) among differentially expressed genes resulting from temperature stress treatments, suggesting that the heat shock response plays a crucial role in enabling B. aeruginosa to cope with environmental stress. In light of these observations, we conducted a comprehensive identification and analysis of the Hsp gene family within B. aeruginosa, discovering that the number of Hsp20 family members was significantly higher in this species compared to other closely related organisms-corresponding with our previous analyses regarding gene family expansion and contraction.</p><p><strong>Conclusions: </strong>This study has yielded a high-quality genome of B. aeruginosa and provided insights into the potential molecular mechanisms that underpin the organism's environmental resilience, and that Hsps in this species are sensitive to environmental stresses and play a key role in its stress mechanisms.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"23 1","pages":"285"},"PeriodicalIF":4.5,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}