BMC Biology最新文献

{"title":"Berberine ameliorates dextran sulfate sodium -induced colitis through tuft cells and bitter taste signalling.","authors":"Yuxuan Yang, Wenqing Li, Kaineng Sun, Siyu Sun, Yong Zhang, Lin Chen, Yangyue Ni, Min Hou, Zhipeng Xu, Lu Chen, Minjun Ji","doi":"10.1186/s12915-024-02078-7","DOIUrl":"https://doi.org/10.1186/s12915-024-02078-7","url":null,"abstract":"<p><strong>Background: </strong>Inflammatory bowel disease (IBD), a persistent gastrointestinal disease, is featured with impaired gut immunity. Previous studies have demonstrated that tuft cells can regulate the intestinal type 2 immune response by activating downstream ILC2 and Th2 cells and repair gut barrier upon invasion of parasitic helminths, bacteria, protozoans, and enteritis through different chemo-sensing receptors, such as bitter taste receptors. Berberine is a widely used in the treatment of diarrhea in clinic, however the mechanism underlying this effect is not clear. In this study, we aim to explore the relationship between berberine and tuft cells in dextran sulfate sodium (DSS) -induced colitis.</p><p><strong>Results: </strong>Our data showed that berberine significantly ameliorated DSS-induced colitis and regulating type 2 innate immune lymphocytes (ILC2) and Th2 immune cells via tuft cells in the gut. Furthermore, the effect of berberine on colitis was partially abolished by U73122, a bitter taste receptor inhibitor, suggesting that bitter taste signalling pathway played an important role in the effect of berberine on relieving colitis.</p><p><strong>Conclusions: </strong>Berberine ameliorates dextran sulfate sodium -induced colitis through tuft cells and bitter taste signalling. Our study reveals the unique pharmacological mechanisms of berberine in the context of colitis, laying the foundation for further clinical applications of this compound.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"22 1","pages":"280"},"PeriodicalIF":4.4,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11610372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tripartite interactions of PKA catalytic subunit and C-terminal domains of cardiac Ca²⁺ channel may modulate its β-adrenergic regulation.","authors":"Shimrit Oz, Tal Keren-Raifman, Tom Sharon, Suraj Subramaniam, Tamara Pallien, Moshe Katz, Vladimir Tsemakhovich, Anastasiia Sholokh, Baraa Watad, Debi Ranjan Tripathy, Giorgia Sasson, Orna Chomsky-Hecht, Leonid Vysochek, Maike Schulz-Christian, Claudia Fecher-Trost, Kerstin Zühlke, Daniela Bertinetti, Friedrich W Herberg, Veit Flockerzi, Joel A Hirsch, Enno Klussmann, Sharon Weiss, Nathan Dascal","doi":"10.1186/s12915-024-02076-9","DOIUrl":"10.1186/s12915-024-02076-9","url":null,"abstract":"<p><strong>Background: </strong>The β-adrenergic augmentation of cardiac contraction, by increasing the conductivity of L-type voltage-gated Ca_V1.2 channels, is of great physiological and pathophysiological importance. Stimulation of β-adrenergic receptors (βAR) activates protein kinase A (PKA) through separation of regulatory (PKAR) from catalytic (PKAC) subunits. Free PKAC phosphorylates the inhibitory protein Rad, leading to increased Ca²⁺ influx. In cardiomyocytes, the core subunit of Ca_V1.2, Ca_V1.2α₁, exists in two forms: full-length or truncated (lacking the distal C-terminus (dCT)). Signaling efficiency is believed to emanate from protein interactions within multimolecular complexes, such as anchoring PKA (via PKAR) to Ca_V1.2α₁ by A-kinase anchoring proteins (AKAPs). However, AKAPs are inessential for βAR regulation of Ca_V1.2 in heterologous models, and their role in cardiomyocytes also remains unclear.</p><p><strong>Results: </strong>We show that PKAC interacts with Ca_V1.2α₁ in heart and a heterologous model, independently of Rad, PKAR, or AKAPs. Studies with peptide array assays and purified recombinant proteins demonstrate direct binding of PKAC to two domains in Ca_V1.2α₁-CT: the proximal and distal C-terminal regulatory domains (PCRD and DCRD), which also interact with each other. Data indicate both partial competition and possible simultaneous interaction of PCRD and DCRD with PKAC. The βAR regulation of Ca_V1.2α₁ lacking dCT (which harbors DCRD) was preserved, but subtly altered, in a heterologous model, the Xenopus oocyte.</p><p><strong>Conclusions: </strong>We discover direct interactions between PKAC and two domains in Ca_V1.2α₁. We propose that these tripartite interactions, if present in vivo, may participate in organizing the multimolecular signaling complex and fine-tuning the βAR effect in cardiomyocytes.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"22 1","pages":"276"},"PeriodicalIF":4.4,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11603854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The neglected burden of chronic hypoxia on the resistance of glioblastoma multiforme to first-line therapies.","authors":"Jolie Bou-Gharios, Georges Noël, Hélène Burckel","doi":"10.1186/s12915-024-02075-w","DOIUrl":"10.1186/s12915-024-02075-w","url":null,"abstract":"<p><p>Glioblastoma multiforme (GBM) is the most common adult primary brain tumor. The standard of care involves maximal surgery followed by radiotherapy and concomitant chemotherapy with temozolomide (TMZ), in addition to adjuvant TMZ. However, the recurrence rate of GBM within 1-2 years post-diagnosis is still elevated and has been attributed to the accumulation of multiple factors including the heterogeneity of GBM, genomic instability, angiogenesis, and chronic tumor hypoxia. Tumor hypoxia activates downstream signaling pathways involved in the adaptation of GBM to the newly oxygen-deprived environment, thereby contributing to the resistance and recurrence phenomena, despite the multimodal therapeutic approach used to eradicate the tumor. Therefore, in this review, we will focus on the development and implication of chronic or limited-diffusion hypoxia in tumor persistence through genetic and epigenetic modifications. Then, we will detail the hypoxia-induced activation of vital biological pathways and mechanisms that contribute to GBM resistance. Finally, we will discuss a proteomics-based approach to encourage the implication of personalized GBM treatments based on a hypoxia signature.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"22 1","pages":"278"},"PeriodicalIF":4.4,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11603919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome DNA methylomes of tree shrew brains reveal conserved and divergent roles of DNA methylation on sex chromosome regulation.","authors":"Dongmin R Son, Yifan Kong, Yulian Tan, Ting Hu, Lei Shi, Soojin V Yi","doi":"10.1186/s12915-024-02071-0","DOIUrl":"10.1186/s12915-024-02071-0","url":null,"abstract":"<p><strong>Background: </strong>The tree shrew (Tupaia belangeri) is a promising emerging model organism in biomedical studies, notably due to their evolutionary proximity to primates. To enhance our understanding of how DNA methylation is implicated in regulation of gene expression and the X chromosome inactivation (XCI) in tree shrew brains, here we present their first genome-wide, single-base-resolution methylomes integrated with transcriptomes from prefrontal cortices.</p><p><strong>Results: </strong>Genome-wide relationships between DNA methylation and gene expression are consistent with those in other mammals. Interestingly, we observed a clear and significant global reduction (hypomethylation) of DNA methylation across the entire female X chromosome compared to male X. Female hypomethylation does not directly contribute to the gene silencing of the inactivated X chromosome nor does it significantly drive sex-specific gene expression in tree shrews. However, we identified a putative regulatory region in the 5' end of the X-inactive-specific transcript (Xist) gene, whose pattern of differential DNA methylation strongly relate to its sex-differential expression in tree shrews. Furthermore, differential methylation of this region is conserved across different species. We also provide evidence suggesting that the observed difference between human and tree shrew X-linked promoter methylation is associated with the difference in genomic CpG contents.</p><p><strong>Conclusions: </strong>Our study offers novel information on genomic DNA methylation of tree shrews as well as insights into the evolution of sex chromosome regulation in mammals. Specifically, we show conserved role of DNA methylation in regulation of Xist expression and propose genomic CpG contents as a factor in driving sex-differential DNA methylation of X-linked promoters.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"22 1","pages":"277"},"PeriodicalIF":4.4,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11603898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple losses of aKRAB from PRDM9 coincide with a teleost-specific intron size distribution.","authors":"Ann-Christin Zinner, Lars Martin Jakt","doi":"10.1186/s12915-024-02059-w","DOIUrl":"10.1186/s12915-024-02059-w","url":null,"abstract":"<p><strong>Background: </strong>Primary transcripts are largely comprised of intronic sequences that are excised and discarded shortly after synthesis. In vertebrates, the shape of the intron size distribution is largely constant; however, most teleost fish have a diverged log-bimodal 'teleost distribution' (TD) that is seen only in teleosts. How the TD evolved and to what extent this was affected by adaptative or non-adaptive mechanisms is unknown.</p><p><strong>Results: </strong>Here, we show that the TD has evolved independently at least six times and that its appearance is linked to the loss of the aKRAB domain from PRDM9. We determined intron size distributions and identified PRDM9 orthologues from annotated genomes in addition to scanning 1193 teleost assemblies for the aKRAB domain. We show that a diverged form of PRDM9 ( <math><mi>β</mi></math> ) is predominant in teleosts whereas the <math><mi>α</mi></math> version is absent from most species. Only a subset of PRDM9- <math><mi>α</mi></math> proteins contain aKRAB, and hence, it is present only in a small number of teleost lineages. Almost all lineages lacking aKRAB (but no species with) had TDs.</p><p><strong>Conclusions: </strong>In mammals, PRDM9 defines the sites of meiotic recombination through a mechanism that increases structural variance and depends on aKRAB. The loss of aKRAB is likely to have shifted the locations of both recombination and structural variance hotspots. Our observations suggest that the TD evolved as a side-effect of these changes and link recombination to the evolution of intron size illustrating how genome architectures can evolve in the absence of selection.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"22 1","pages":"275"},"PeriodicalIF":4.4,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11600626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel human protein-coding locus identified using a targeted RNA enrichment technique.","authors":"Lu Tang, Dongyang Xu, Lingcong Luo, Weiyan Ma, Xiaojie He, Yong Diao, Rongqin Ke, Philipp Kapranov","doi":"10.1186/s12915-024-02069-8","DOIUrl":"10.1186/s12915-024-02069-8","url":null,"abstract":"<p><strong>Background: </strong>Accurate and comprehensive genomic annotation, including the full list of protein-coding genes, is vital for understanding the molecular mechanisms of human biology. We have previously shown that the genome contains a multitude of yet hidden functional exons and transcripts, some of which might represent novel mRNAs. These results resonate with those from other groups and strongly argue that two decades after the completion of the first draft of the human genome sequence, the current annotation of human genes and transcripts remains far from being complete.</p><p><strong>Results: </strong>Using a targeted RNA enrichment technique, we showed that one of the novel functional exons previously discovered by us and currently annotated as part of a long non-coding RNA, is actually a part of a novel protein-coding gene, InSETG-4, which encodes a novel human protein with no known homologs or motifs. We found that InSETG-4 is induced by various DNA-damaging agents across multiple cell types and therefore might represent a novel component of DNA damage response. Despite its low abundance in bulk cell populations, InSETG-4 exhibited expression restricted to a small fraction of cells, as demonstrated by the amplification-based single-molecule fluorescence in situ hybridization (asmFISH) analysis.</p><p><strong>Conclusions: </strong>This study argues that yet undiscovered human protein-coding genes exist and provides an example of how targeted RNA enrichment techniques can help to fill this major gap in our knowledge of the information encoded in the human genome.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"22 1","pages":"273"},"PeriodicalIF":4.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological evidence of sarbecovirus exposure along Sunda pangolin trafficking pathways.","authors":"Brian M Worthington, Portia Y-H Wong, Kishoree K Kumaree, Tracey-Leigh Prigge, Kar Hon Ng, Yunshi Liao, Paolo Martelli, Sarah Churgin, Foo K Lee, Chris Perkins, Michael Bradley, Mac P Pierce, Marcus H-H Shum, Elliott F Miot, William Y-M Cheung, Shelby E McIlroy, Helen C Nash, Wirdateti, Gono Semiadi, Chee-Wah Tan, Lin-Fa Wang, Gary Ades, David M Baker, Caroline Dingle, Oliver G Pybus, Edward C Holmes, Gabriel M Leung, Yi Guan, Huachen Zhu, Timothy C Bonebrake, Tommy T Y Lam","doi":"10.1186/s12915-024-02074-x","DOIUrl":"10.1186/s12915-024-02074-x","url":null,"abstract":"<p><strong>Background: </strong>Early in the coronavirus disease 2019 (COVID-19) pandemic, Sunda pangolins (Manis javanica) involved in the illegal wildlife trade in mainland China were identified as hosts of severe acute respiratory syndrome-related coronaviruses (SARSr-CoVs). Although it is unconfirmed whether pangolins or other traded wildlife served as intermediate hosts for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the trafficking of pangolins presents a clear risk for transmission of viruses with zoonotic and epizootic potential regardless. We have investigated the origins of pangolin carcasses seized in Hong Kong and have evaluated their potential exposure to SARSr-CoVs, other coronaviruses, and paramyxoviruses, aiming to address a gap in our knowledge with regard to the role of wildlife trade in the maintenance and emergence of pathogens with zoonotic and epizootic potential.</p><p><strong>Results: </strong>Using a combination of virological and wildlife forensics tools, we investigated 89 Sunda pangolin carcasses seized by Hong Kong authorities during anti-smuggling operations in the territory conducted in 2013 (n = 1) and 2018 (n = 88). Swabs, organ tissues, blood, and other body fluids were collected during post-mortem examination. Two enzyme-linked immunosorbent assays (ELISAs), which employ a double-antigen sandwich format, were used to detect antibodies reactive against SARSr-CoVs. One individual was found to be seropositive with support from both methods, while five individuals exhibited a putatively seropositive result from one ELISA method. Polymerase chain reaction (PCR) screening for coronavirus and paramyxovirus ribonucleic acid (RNA) did not yield any positives. Based on genomic data, the seropositive individual was determined to have likely originated from Java, while the putatively seropositive individuals were determined to have originated from populations in Borneo, Java, and Singapore/Sumatra.</p><p><strong>Conclusions: </strong>While the role of pangolins in the evolution and ecology of SARS-CoV-2 is uncertain, our results suggest susceptibility and potential exposure of pangolins to SARSr-CoVs, occurring naturally or associated with the illegal trafficking of these animals. Complex dynamics between natural populations, traded individuals, and pathogen susceptibility complicate conclusions about the role of pangolins, as well as other host species, in the ecology of SARSr-CoVs and potentially zoonotic viruses with risk of future emergence.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"22 1","pages":"274"},"PeriodicalIF":4.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11600613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bayesian tip-dated timeline for diversification and major biogeographic events in Muroidea (Rodentia), the largest mammalian radiation.","authors":"Raquel López-Antoñanzas, Tiago R Simões, Fabien L Condamine, Moritz Dirnberger, Pablo Peláez-Campomanes","doi":"10.1186/s12915-024-02053-2","DOIUrl":"10.1186/s12915-024-02053-2","url":null,"abstract":"<p><strong>Background: </strong>Extinct organisms provide vital information about the time of origination and biogeography of extant groups. The development of phylogenetic methods to study evolutionary processes through time has revolutionized the field of evolutionary biology and led to an unprecedented expansion of our knowledge of the tree of life. Recent developments applying Bayesian approaches, using fossil taxa as tips to be included alongside their living relatives, have revitalized the use of morphological data in evolutionary tree inferences. Eumuroida rodents represent the largest group of mammals including more than a quarter of all extant mammals and have a rich fossil record spanning the last ~ 45 million years. Despite this wealth of data, our current understanding of the classification, major biogeographic patterns, and divergence times for this group comes from molecular phylogenies that use fossils only as a source of node calibrations. However, node calibrations impose several constraints on tree topology and must necessarily make a priori assumptions about the placement of fossil taxa without testing their placement in the tree.</p><p><strong>Results: </strong>We present the first morphological dataset with extensive fossil sampling for Muroidea. By applying Bayesian morphological clocks with tip dating and process-based biogeographic models, we provide a novel hypothesis for muroid relationships and revised divergence times for the clade that incorporates uncertainty in the placement of all fossil species. Even under strong violation of the clock model, we found strong congruence between results for divergence times, providing a robust timeline for muroid diversification. This new timeline was used for biogeographic analyses, which revealed a dynamic scenario mostly explained by dispersal events between and within the Palearctic and North African regions.</p><p><strong>Conclusions: </strong>Our results provide important insights into the evolution of Muroidea rodents and clarify the evolutionary pathways of their main lineages. We exploited the advantage of tip dating Bayesian approaches in morphology-based datasets and provided a classification of the largest superfamily of mammals resulting from robust phylogenetic inference, inferring the biogeographical history, diversification, and divergence times of its major lineages.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"22 1","pages":"270"},"PeriodicalIF":4.4,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The golden genome annotation of Ganoderma lingzhi reveals a more complex scenario of eukaryotic gene structure and transcription activity.","authors":"Lining Wang, Peiqi Shi, Zhaohua Ping, Qinghua Huang, Liqun Jiang, Nianfang Ma, Qingfu Wang, Jiang Xu, Yajie Zou, Zhihai Huang","doi":"10.1186/s12915-024-02073-y","DOIUrl":"10.1186/s12915-024-02073-y","url":null,"abstract":"<p><strong>Background: </strong>It is generally accepted that nuclear genes in eukaryotes are located independently on chromosomes and expressed in a monocistronic manner. However, accumulating evidence suggests a more complex landscape of gene structure and transcription. Ganoderma lingzhi, a model medicinal fungus, currently lacks high-quality genome annotation, hindering genetic studies.</p><p><strong>Results: </strong>Here, we reported a golden annotation of G. lingzhi, featuring 14,147 high-confidence genes derived from extensive manual corrections. Novel characteristics of gene structure and transcription were identified accordingly. Notably, non-canonical splicing sites accounted for 1.99% of the whole genome, with the predominant types being GC-AG (1.85%), GT-AC (0.05%), and GT-GG (0.04%). 1165 pairs of genes were found to have overlapped transcribed regions, and 92.19% of which showed opposite directions of gene transcription. A total of 5,412,158 genetic variations were identified among 13 G. lingzhi strains, and the manually corrected gene sets resulted in enhanced functional annotation of these variations. More than 60% of G. lingzhi genes were alternatively spliced. In addition, we found that two or more protein-coding genes (PCGs) can be transcribed into a single RNA molecule, referred to as polycistronic genes. In total, 1272 polycistronic genes associated with 2815 PCGs were identified.</p><p><strong>Conclusions: </strong>The widespread presence of polycistronic genes in G. lingzhi strongly complements the theory that polycistron is also present in eukaryotic genomes. The extraordinary gene structure and transcriptional activity uncovered through this golden annotation provide implications for the study of genes, genomes, and related studies in G. lingzhi and other eukaryotes.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"22 1","pages":"271"},"PeriodicalIF":4.4,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature-dependent dynamics of energy stores in Drosophila.","authors":"Diana Knoblochová, Malleswara Dharanikota, Martina Gáliková, Peter Klepsatel","doi":"10.1186/s12915-024-02072-z","DOIUrl":"10.1186/s12915-024-02072-z","url":null,"abstract":"<p><strong>Background: </strong>Understanding how ectotherms manage energy in response to temperature is crucial for predicting their responses to climate change. However, the complex interplay between developmental and adult thermal conditions on total energy stores remains poorly understood. Here, we present the first comprehensive quantification of this relationship in Drosophila melanogaster, a model ectotherm, across its entire thermal tolerance range. To account for potential intraspecific variation, we used flies from two distinct populations originating from different climate zones. Utilizing a full factorial design, we assessed the effects of both developmental and adult temperatures on the amount of key energy macromolecules (fat, glycogen, trehalose, and glucose). Importantly, by quantifying these macromolecules, we were able to calculate the total available energy.</p><p><strong>Results: </strong>Our findings reveal that the dynamic interplay between developmental and adult temperatures profoundly influences the energy balance in Drosophila. The total energy reserves exhibited a quadratic response to adult temperature, with an optimal range of 18-21 °C for maximizing energy levels. Additionally, the temperature during development considerably affected maximum energy stores, with the highest reserves observed at a developmental temperature of approximately 20-21 °C. Deviations from this relatively narrow optimal thermal range markedly reduced energy stores, with each 1 °C increase above 25 °C diminishing energy reserves by approximately 15%.</p><p><strong>Conclusions: </strong>This study highlights the critical and interacting roles of both developmental and adult thermal conditions in shaping Drosophila energy reserves, with potentially profound implications for fitness, survival, and ecological interactions under future climate scenarios.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":"22 1","pages":"272"},"PeriodicalIF":4.4,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}