Magnetic resonance (Gottingen, Germany)最新文献

{"title":"Conformational features and ionization states of Lys side chains in a protein studied using the stereo-array isotope labeling (SAIL) method.","authors":"Mitsuhiro Takeda,&nbsp;Yohei Miyanoiri,&nbsp;Tsutomu Terauchi,&nbsp;Masatsune Kainosho","doi":"10.5194/mr-2-223-2021","DOIUrl":"10.5194/mr-2-223-2021","url":null,"abstract":"<p><p>Although both the <i>hydrophobic</i> aliphatic chain and <i>hydrophilic</i> <math><mi>ζ</mi></math>-amino group of the Lys side chain presumably contribute to the structures and functions of proteins, the <i>dual</i> nature of the Lys residue has not been fully investigated using NMR spectroscopy, due to the lack of appropriate methods to acquire comprehensive information on its long consecutive methylene chain. We describe herein a robust strategy to address the current situation, using various isotope-aided NMR technologies. The feasibility of our approach is demonstrated for the <math><mrow><mi>Δ</mi><mo>+</mo></mrow></math>PHS/V66K variant of staphylococcal nuclease (SNase), which contains 21 Lys residues, including the engineered Lys-66 with an unusually low p<math><mrow><msub><mi>K</mi><mi>a</mi></msub></mrow></math> of <math><mo>∼</mo></math> 5.6. All of the NMR signals for the 21 Lys residues were sequentially and stereospecifically assigned using the stereo-array isotope-labeled Lys (SAIL-Lys), [U-<math><msup><mi></mi><mn>13</mn></msup></math>C,<math><msup><mi></mi><mn>15</mn></msup></math>N; <math><mrow><msub><mi>β</mi><mn>2</mn></msub></mrow></math>,<math><mrow><msub><mi>γ</mi><mn>2</mn></msub></mrow></math>,<math><mrow><msub><mi>δ</mi><mn>2</mn></msub></mrow></math>,<math><mrow><msub><mi>ε</mi><mn>3</mn></msub></mrow></math>-D<math><msub><mi></mi><mn>4</mn></msub></math>]-Lys. The complete set of assigned <math><msup><mi></mi><mn>1</mn></msup></math>H, <math><msup><mi></mi><mn>13</mn></msup></math>C, and <math><msup><mi></mi><mn>15</mn></msup></math>N NMR signals for the Lys side-chain moieties affords useful structural information. For example, the set includes the characteristic chemical shifts for the <math><msup><mi></mi><mn>13</mn></msup></math>C<math><msup><mi></mi><mi>δ</mi></msup></math>, <math><msup><mi></mi><mn>13</mn></msup></math>C<math><msup><mi></mi><mi>ε</mi></msup></math>, and <math><msup><mi></mi><mn>15</mn></msup></math>N<math><msup><mi></mi><mi>ζ</mi></msup></math> signals for Lys-66, which has the deprotonated <math><mi>ζ</mi></math>-amino group, and the large upfield shifts for the <math><msup><mi></mi><mn>1</mn></msup></math>H and <math><msup><mi></mi><mn>13</mn></msup></math>C signals for the Lys-9, Lys-28, Lys-84, Lys-110, and Lys-133 side chains, which are indicative of nearby aromatic rings. The <math><msup><mi></mi><mn>13</mn></msup></math>C<math><msup><mi></mi><mi>ε</mi></msup></math> and <math><msup><mi></mi><mn>15</mn></msup></math>N<math><msup><mi></mi><mi>ζ</mi></msup></math> chemical shifts of the SNase variant selectively labeled with either [<math><mi>ε</mi></math>-<math><msup><mi></mi><mn>13</mn></msup></math>C;<math><mi>ε</mi></math>,<math><mi>ε</mi></math>-D<math><msub><mi></mi><mn>2</mn></msub></math>]-Lys or SAIL-Lys, dissolved in H<math><msub><mi></mi><mn>2</mn></msub></math>O and D<math><msub><mi></mi><mn>2</mn></msub></math>O, showed that the deuterium-induced shifts for Lys-66 were sub","PeriodicalId":93333,"journal":{"name":"Magnetic resonance (Gottingen, Germany)","volume":"2 1","pages":"223-237"},"PeriodicalIF":0.0,"publicationDate":"2021-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71415870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"When the MOUSE leaves the house.","authors":"Bernhard Blümich,&nbsp;Jens Anders","doi":"10.5194/mr-2-149-2021","DOIUrl":"10.5194/mr-2-149-2021","url":null,"abstract":"<p><p>Change is inherent to time being transient. With the NMR-MOUSE (MObile Universal Surface Explorer) having matured into an established NMR tool for nondestructive testing of materials, this forward-looking retrospective assesses the challenges the NMR-MOUSE faced when deployed outside a protected laboratory and how its performance quality can be maintained and improved when operated under adverse conditions in foreign environments. This work is dedicated to my dear colleague and friend Geoffrey Bodenhausen on the occasion of his crossing an honorable timeline in appreciation of his ever-continuing success of fueling the dynamics of magnetic resonance.</p>","PeriodicalId":93333,"journal":{"name":"Magnetic resonance (Gottingen, Germany)","volume":"2 1","pages":"149-160"},"PeriodicalIF":0.0,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43845303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The decay of the refocused Hahn echo in double electron-electron resonance (DEER) experiments.","authors":"Thorsten Bahrenberg, Samuel M Jahn, Akiva Feintuch, Stefan Stoll, Daniella Goldfarb","doi":"10.5194/mr-2-161-2021","DOIUrl":"10.5194/mr-2-161-2021","url":null,"abstract":"<p><p>Double electron-electron resonance (DEER) is a pulse electron paramagnetic resonance (EPR) technique that measures distances between paramagnetic centres. It utilizes a four-pulse sequence based on the refocused Hahn spin echo. The echo decays with increasing pulse sequence length <math><mrow><mn>2</mn><mo>(</mo><msub><mi>τ</mi><mn>1</mn></msub><mo>+</mo><msub><mi>τ</mi><mn>2</mn></msub><mo>)</mo></mrow></math>, where <math><mrow><msub><mi>τ</mi><mn>1</mn></msub></mrow></math> and <math><mrow><msub><mi>τ</mi><mn>2</mn></msub></mrow></math> are the two time delays. In DEER, the value of <math><mrow><msub><mi>τ</mi><mn>2</mn></msub></mrow></math> is determined by the longest inter-spin distance that needs to be resolved, and <math><mrow><msub><mi>τ</mi><mn>1</mn></msub></mrow></math> is adjusted to maximize the echo amplitude and, thus, sensitivity. We show experimentally that, for typical spin centres (nitroxyl, trityl, and Gd(III)) diluted in frozen protonated solvents, the largest refocused echo amplitude for a given <math><mrow><msub><mi>τ</mi><mn>2</mn></msub></mrow></math> is obtained neither at very short <math><mrow><msub><mi>τ</mi><mn>1</mn></msub></mrow></math> (which minimizes the pulse sequence length) nor at <math><mrow><msub><mi>τ</mi><mn>1</mn></msub><mo>=</mo><msub><mi>τ</mi><mn>2</mn></msub></mrow></math> (which maximizes dynamic decoupling for a given total sequence length) but rather at <math><mrow><msub><mi>τ</mi><mn>1</mn></msub></mrow></math> values smaller than <math><mrow><msub><mi>τ</mi><mn>2</mn></msub></mrow></math>. Large-scale spin dynamics simulations based on the coupled cluster expansion (CCE), including the electron spin and several hundred neighbouring protons, reproduce the experimentally observed behaviour almost quantitatively. They show that electron spin dephasing is driven by solvent protons via the flip-flop coupling among themselves and their hyperfine couplings to the electron spin.</p>","PeriodicalId":93333,"journal":{"name":"Magnetic resonance (Gottingen, Germany)","volume":"2 1","pages":"161-173"},"PeriodicalIF":0.0,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539729/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71415889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Four-dimensional NOE-NOE spectroscopy of SARS-CoV-2 Main Protease to facilitate resonance assignment and structural analysis.","authors":"Angus J Robertson, Jinfa Ying, Ad Bax","doi":"10.5194/mr-2-129-2021","DOIUrl":"10.5194/mr-2-129-2021","url":null,"abstract":"<p><p>Resonance assignment and structural studies of larger proteins by nuclear magnetic resonance (NMR) can be challenging when exchange broadening, multiple stable conformations, and <math><msup><mi></mi><mn>1</mn></msup></math>H back-exchange of the fully deuterated chain pose problems. These difficulties arise for the SARS-CoV-2 Main Protease, a homodimer of 2 <math><mo>×</mo></math> 306 residues. We demonstrate that the combination of four-dimensional (4D) TROSY-NOESY-TROSY spectroscopy and 4D NOESY-NOESY-TROSY spectroscopy provides an effective tool for delineating the <math><msup><mi></mi><mn>1</mn></msup></math>H-<math><msup><mi></mi><mn>1</mn></msup></math>H dipolar relaxation network. In combination with detailed structural information obtained from prior X-ray crystallography work, such data are particularly useful for extending and validating resonance assignments as well as for probing structural features.</p>","PeriodicalId":93333,"journal":{"name":"Magnetic resonance (Gottingen, Germany)","volume":" ","pages":"129-138"},"PeriodicalIF":0.0,"publicationDate":"2021-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44528067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exchange interaction in short-lived flavine adenine dinucleotide biradical in aqueous solution revisited by CIDNP (chemically induced dynamic nuclear polarization) and nuclear magnetic relaxation dispersion.","authors":"Ivan V Zhukov, Alexey S Kiryutin, Mikhail S Panov, Natalya N Fishman, Olga B Morozova, Nikita N Lukzen, Konstantin L Ivanov, Hans-Martin Vieth, Renad Z Sagdeev, Alexandra V Yurkovskaya","doi":"10.5194/mr-2-139-2021","DOIUrl":"10.5194/mr-2-139-2021","url":null,"abstract":"<p><p>Flavin adenine dinucleotide (FAD) is an important cofactor in many light-sensitive enzymes. The role of the adenine moiety of FAD in light-induced electron transfer was obscured, because it involves an adenine radical, which is short-lived with a weak chromophore. However, an intramolecular electron transfer from adenine to flavin was revealed several years ago by Robert Kaptein by using chemically induced dynamic nuclear polarization (CIDNP). The question of whether one or two types of biradicals of FAD in aqueous solution are formed stays unresolved so far. In the present work, we revisited the CIDNP study of FAD using a robust mechanical sample shuttling setup covering a wide magnetic field range with sample illumination by a light-emitting diode. Also, a cost efficient fast field cycling apparatus with high spectral resolution detection up to 16.4 T for nuclear magnetic relaxation dispersion studies was built based on a 700 MHz NMR spectrometer. Site-specific proton relaxation dispersion data for FAD show a strong restriction of the relative motion of its isoalloxazine and adenine rings with coincident correlation times for adenine, flavin, and their ribityl phosphate linker. This finding is consistent with the assumption that the molecular structure of FAD is rigid and compact. The structure with close proximity of the isoalloxazine and purine moieties is favorable for reversible light-induced intramolecular electron transfer from adenine to triplet excited flavin with formation of a transient spin-correlated triplet biradical F<math><msup><mi></mi><mrow><mi>⚫</mi><mo>-</mo></mrow></msup></math>-A<math><msup><mi></mi><mrow><mi>⚫</mi><mo>+</mo></mrow></msup></math>. Spin-selective recombination of the biradical leads to the formation of CIDNP with a common emissive maximum at 4.0 mT detected for adenine and flavin protons. Careful correction of the CIDNP data for relaxation losses during sample shuttling shows that only a single maximum of CIDNP is formed in the magnetic field range from 0.1 mT to 9 T; thus, only one type of FAD biradical is detectable. Modeling of the CIDNP field dependence provides good agreement with the experimental data for a normal distance distribution between the two radical centers around 0.89 nm and an effective electron exchange interaction of <math><mo>-</mo></math>2.0 mT.</p>","PeriodicalId":93333,"journal":{"name":"Magnetic resonance (Gottingen, Germany)","volume":"2 1","pages":"139-148"},"PeriodicalIF":0.0,"publicationDate":"2021-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71415873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pd-based bimetallic catalysts for parahydrogen-induced polarization in heterogeneous hydrogenations.","authors":"Dudari B Burueva, Aleksandr Y Stakheev, Igor V Koptyug","doi":"10.5194/mr-2-93-2021","DOIUrl":"10.5194/mr-2-93-2021","url":null,"abstract":"<p><p>Production of hyperpolarized catalyst-free gases and liquids by heterogeneous hydrogenation with parahydrogen can be useful for various technical as well as biomedical applications, including in vivo studies, investigations of mechanisms of industrially important catalytic processes, enrichment of nuclear spin isomers of polyatomic gases, and more. In this regard, the wide systematic search for heterogeneous catalysts effective in pairwise <math><mrow><msub><mi>H</mi><mn>2</mn></msub></mrow></math> addition required for the observation of parahydrogen-induced polarization (PHIP) effects is crucial. Here in this work we demonstrate the competitive advantage of Pd-based bimetallic catalysts for PHIP in heterogeneous hydrogenations (HET-PHIP). The dilution of catalytically active Pd with less active Ag or In atoms provides the formation of atomically dispersed <math><mrow><msub><mi>Pd</mi><mn>1</mn></msub></mrow></math> sites on the surface of Pd-based bimetallic catalysts, which are significantly more selective toward pairwise <math><mrow><msub><mi>H</mi><mn>2</mn></msub></mrow></math> addition compared to the monometallic Pd. Furthermore, the choice of the dilution metal (Ag or In) has a pronounced effect on the efficiency of bimetallic catalysts in HET-PHIP, as revealed by comparing Pd-Ag and Pd-In bimetallic catalysts.</p>","PeriodicalId":93333,"journal":{"name":"Magnetic resonance (Gottingen, Germany)","volume":"2 1","pages":"93-103"},"PeriodicalIF":0.0,"publicationDate":"2021-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71415881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extending the applicability of P3D for structure determination of small molecules.","authors":"Alain Ibáñez de Opakua,&nbsp;Markus Zweckstetter","doi":"10.5194/mr-2-105-2021","DOIUrl":"10.5194/mr-2-105-2021","url":null,"abstract":"<p><p>The application of anisotropic nuclear magnetic resonance (NMR) parameters for the correct structural assignment of small molecules requires the use of partially ordered media. Previously we demonstrated that the use of P3D simulations using poly(<math><mi>γ</mi></math>-benzyl-L-glutamate) (PBLG) as an alignment medium allows for the determination of the correct diastereomer from extremely sparse NMR data. Through the analysis of the structural characteristics of small molecules in different alignment media, here we show that when steric or electrostatic factors dominate the alignment, P3D-PBLG retains its diastereomer discrimination power. We also demonstrate that P3D simulations can define the different conformations of a flexible small molecule from sparse NMR data.</p>","PeriodicalId":93333,"journal":{"name":"Magnetic resonance (Gottingen, Germany)","volume":"2 1","pages":"105-116"},"PeriodicalIF":0.0,"publicationDate":"2021-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71415875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simple rules for resolved level-crossing spectra in magnetic field effects on reaction yields.","authors":"Dmitri V Stass,&nbsp;Victor A Bagryansky,&nbsp;Yuri N Molin","doi":"10.5194/mr-2-77-2021","DOIUrl":"10.5194/mr-2-77-2021","url":null,"abstract":"<p><p>In this work we derive conditions under which a level-crossing line in a magnetic field effect curve for a recombining radical pair will be equivalent to the electron spin resonance (ESR) spectrum and discuss three simple rules for qualitative prediction of the level-crossing spectra.</p>","PeriodicalId":93333,"journal":{"name":"Magnetic resonance (Gottingen, Germany)","volume":"2 1","pages":"77-91"},"PeriodicalIF":0.0,"publicationDate":"2021-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71415886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of coherent re-arrangement for long-lived spin order","authors":"F. Teleanu, P. Vasos","doi":"10.5194/MR-2021-36","DOIUrl":"https://doi.org/10.5194/MR-2021-36","url":null,"abstract":"Abstract. Long-lived spin order-based approaches for magnetic resonance rely on the transition between two magnetic environments of different symmetry, one governed by the magnetic field of the spectrometer and the other where this strong magnetic field is inconsequential. Research on the excitation of magnetic-symmetry transitions in nuclear spins is a scientific field that debuted in Southampton in the years 2000. We advanced in this field carrying the baggage of pre-established directions in NMR spectroscopy. We propose to reveal in this text the part of discoveries that may have been obscured by our choice to only look at them through the experience of such pre-established directions, at the time. Focussing on potential applications, we may have insufficiently emphasised in the manuscripts the methodological developments that necessitated most scientific effort. Such methods developments foster most of the progress in NMR. Thus, we present the contributed mechanisms of translation between the symmetric and non-symmetric environments with respect to the main magnetic field B0, free of any utilitarian perspective. The concept of zero-quantum rotations in the starting blocks of long-lived state populations, magnetisation transfers between hyperpolarised heteronuclei and protons, and selective inversion for long-lived coherences are discussed, as well as hybrid 2D methods based on both insensitive nuclei excitation (“INEPT”) and long-lived spin order. We can see at this point that these magnetic wheels will take a longer time than we initially thought to set in motion new applications in studies of slow diffusion, angiography, or large-protein structure. However, these pulse sequences seed subsequent magnetic mechanisms that are sure to contribute to applications. For instance, some of the introduced coherence rotations were combined with classical pulse blocks to obtain 2D correlations between protons and heteronuclei. We hope the pulse sequence building blocks discussed herein open further perspectives for magnetic resonance experiments with long-lived spin order.\u0000","PeriodicalId":93333,"journal":{"name":"Magnetic resonance (Gottingen, Germany)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42466265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small-molecule inhibitors of the PDZ domain of Dishevelled \u0000proteins interrupt Wnt signalling","authors":"N. Kamdem, Y. Roske, D. Kovalskyy, M. Platonov, O. Balinskyi, A. Kreuchwig, J. Saupe, L. Fang, A. Diehl, P. Schmieder, G. Krause, J. Rademann, U. Heinemann, W. Birchmeier, H. Oschkinat","doi":"10.2210/pdb6zc7/pdb","DOIUrl":"https://doi.org/10.2210/pdb6zc7/pdb","url":null,"abstract":"Abstract. Dishevelled (Dvl) proteins are important regulators of the Wnt signalling pathway, interacting through their PDZ domains with the Wnt receptor Frizzled. Blocking the Dvl PDZ/Frizzled interaction represents a potential approach for cancer treatment, which stimulated the identification of small molecule inhibitors, among them the anti-inflammatory drug Sulindac and Ky-02327. Aiming to develop tighter binding compounds without side effects, we investigated structure-activity relationships of sulfonamides. X-ray crystallography showed high complementarity of anthranilic acid derivatives in the GLGF loop cavity and space for ligand growth towards the PDZ surface. Our best binding compound inhibits Wnt signalling in a dose-dependent manner as demonstrated by TOP-GFP assays (IC50 ~50 µM), and Western blotting of β-catenin levels. Real-time PCR showed reduction in the expression of Wnt-specific genes. Our compound interacted with Dvl-1 PDZ (Kd = 2.4 µM) stronger than Ky-02327 and may be developed into a lead compound interfering with the Wnt pathway.\u0000","PeriodicalId":93333,"journal":{"name":"Magnetic resonance (Gottingen, Germany)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47942992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}