Bioorganicheskaia khimiia最新文献_第6页

[Immunoassay of nine serological tumor markers on hydrogel-based microchip]. [9种血清学肿瘤标志物在水凝胶微芯片上的免疫分析]。

Bioorganicheskaia khimiia Pub Date : 2013-11-01

Zh I Zubcova, E N Savvateeva, V I Butvilovskaia, M V Cybul'skaia, V R Chechetkin, L O Samokhina, L I Vinnitskiĭ, V V Maslennikov, Iu P Reznikov, A S Zasedatelev, A Iu Rubina

{"title":"[Immunoassay of nine serological tumor markers on hydrogel-based microchip].","authors":"Zh I Zubcova, E N Savvateeva, V I Butvilovskaia, M V Cybul'skaia, V R Chechetkin, L O Samokhina, L I Vinnitskiĭ, V V Maslennikov, Iu P Reznikov, A S Zasedatelev, A Iu Rubina","doi":"","DOIUrl":"","url":null,"abstract":"A prototype of test-system for simultaneous quantitative assay of nine tumor markers in blood serum was developed. The main constituent of the test-system is OM-9 biochip containing immobilized antibodies against nine oncomarkers: α-fetoprotein (AFP), carcinoembryonic antigen (CEA), human chorionic gonadotropin (HCG), cancer antigen 15-3 (CA 15-3), cancer antigen 125 (CA 125), cancer antigen 19-9 (CA 19-9), prostate-specific antigen, total (PSAtot) and free (PSAfree) forms, neuron-specific enolase (NSE). The biochip-based assay procedure for carrying out simultaneous quantitative determination of nine tumor markers in patient's blood serum: two-steps sandwich-immunoassay, was proposed. The main analytical characteristics of the method were obtained. The results permit to consider the prototype of the test-system as a promising instrument for clinical application. The test-system prototype was tested using blood serum samples of oncological patients (252 samples) and healthy donors (185 samples). Increased concentrations of one or more tumor markers above the normal level were found in 76.6% cases of oncological patients and only in 6% cases of healthy donors. For colorectal cancer patients group, application of modern statistical methods of data-processing in medical researchers, i.e. ROC-analysis and logistic regression, indicted that the simultaneous assay of nine tumor markers on biochips showed much more diagnostic significance (area under the ROC-curve (AUC) reached 0.84) than traditional assay of 2 tumor markers, CEA and CA 19-9 (AUC = 0.59). The developed biochip-based test-system can be recommended both for the estimation of people's health, e.g., for standard medical examination, and for tracking of tumoral process in postsurgical period or after specific tumor treatment.","PeriodicalId":9325,"journal":{"name":"Bioorganicheskaia khimiia","volume":"39 6","pages":"693-704"},"PeriodicalIF":0.0,"publicationDate":"2013-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33067199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Investigation of active center of deoxynucleoside monophosphate kinase of bacteriophage T5 by site-directed mutagenesis]. 噬菌体T5脱氧核苷单磷酸激酶活性中心的定点诱变研究。

Bioorganicheskaia khimiia Pub Date : 2013-11-01

G V Mikulinskaia, S A Taran, Iu S Skoblov, S A Feofanov

{"title":"[Investigation of active center of deoxynucleoside monophosphate kinase of bacteriophage T5 by site-directed mutagenesis].","authors":"G V Mikulinskaia, S A Taran, Iu S Skoblov, S A Feofanov","doi":"","DOIUrl":"","url":null,"abstract":"Based on the computer model of active center of bacteriophage T5 deoxyribonucleoside monophosphate kinase amino acid residues essential for the enzyme activity were determined. As the result of site-directed mutagenesis, cloning and expression of the gene in E. coli series of proteins were obtained with single amino acid substitutions of conservative active center residues--S13A, D16N, T17N, T17S, R130K, K131E, Q134A, G137A, T138A, W150F, W150A, D170N, R172I, E176Q. Electrophoretically homogeneous preparations of mutant forms were purified using ion exchange and affinity chromatographic steps. Measuring of the specific enzyme activities of these enzymes for the natural acceptors of phosphoryl group (dAMP, dCMP, dGMP, dTMP) revealed that substitutions of charged residues of NMP-binding domain-namely, R130, R172, D170 and E176-lead to almost complete loss of enzyme activity. It was shown that presence of OH-group at position 17 is also important for catalytic activity. Based on the changes in specific activities we suppose that arginine residues at positions 130 and 172 participate in binding of γ-phosphoryl of donor and α-phosphoryl of acceptor. Also, aspartic acid at 16 position of ATP-binding site (P-loop) probably assists in the binding of acceptor, first of all dTMP. Unequal decrease in enzyme activities for different substrates of partially active mutants--G137A, T138A, T17N, Q134A, S13A, D16N--indicate that in the binding of various substrates different amino acid residues take part.","PeriodicalId":9325,"journal":{"name":"Bioorganicheskaia khimiia","volume":"39 6","pages":"680-92"},"PeriodicalIF":0.0,"publicationDate":"2013-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33067200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel urea and thiourea derivatives of thiazole-glutamic acid conjugate as potential inhibitors of microbes and fungi. 噻唑-谷氨酸缀合物的新型尿素和硫脲衍生物作为微生物和真菌的潜在抑制剂。

Bioorganicheskaia khimiia Pub Date : 2013-11-01

A Sharma, R Suhas, S Chandan, D C Gowda

引用次数: 0

Mulberry (Morus L.) methionine sulfoxide rreductase gene cloning, sequence analysis, and expression in plant development and stress response. 桑树(Morus L.)蛋氨酸亚砜还原酶基因的克隆、序列分析及其在植物发育和胁迫响应中的表达。