[派克肝中α类谷胱甘肽s -转移酶]。

Bioorganicheskaia khimiia Pub Date : 2013-09-01

E V Borvinskaia, L P Smirnov, N N Nemova

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摘要

本研究从白鲑肝脏中分离到谷胱甘肽s -转移酶(GST)，经SDS-PAGE和等电聚焦检测，GST为均质。其亚基质量为25235.36 Da(根据HPLC-MS/MS)， pI约为6.4。测定了底物特异性、热稳定性、部分动力学特性和最佳pH值。该酶被鉴定为Alpha类GST。

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[Glutathione S-transferase of alpha class from pike liver].

In this study, glutathione S-transferase (GST) was isolated from the liver of pike Esox lucius, which was homogenous according to SDS-PAGE and isoelectrofocusing. It is a homodimer with subunits mass 25235.36 Da (according to HPLC-MS/MS) and pI about 6.4. Substrate specificity, thermostability, some kinetic characteristics and optimum pH were determined. The enzyme was identified as Alpha class GST.

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Bioorganicheskaia khimiia

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