[An expedient synthesis of fluorescent labeled ceramide-1-phosphate analogues].

Abstract

A synthesis for fluorescent analogs of ceramide-1-phosphate bearing 9-anthrylvinyl or 4,4-difluoro-3a,4a- diaza-s-indacene-8-yl (Me4-BODIPY) fluorophore at o-position of fatty acid residue was carried out. The key stage of the synthesis is hydrolysis of corresponding sphingomyelins catalyzed by phospholipase D from Streptomyces chromofuscus; the enzymatic yield has been raised to 50-70% by appliance of organic solvent in the incubation medium.