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[Chimeric SHA-D domain ("SH3-Bergerac"): 3D-structure and dynamics studies in solution]. [嵌合SHA-D结构域(“SH3-Bergerac”):溶液中的3d结构和动力学研究]。
Bioorganicheskaia khimiia Pub Date : 2010-07-01
V S Khristophorov, D A Prokhorov, M A Timchenko, Iu A Kudrevatykh, L V Gushchina, V V Filimonov, V P Kutyshenko
{"title":"[Chimeric SHA-D domain (\"SH3-Bergerac\"): 3D-structure and dynamics studies in solution].","authors":"V S Khristophorov,&nbsp;D A Prokhorov,&nbsp;M A Timchenko,&nbsp;Iu A Kudrevatykh,&nbsp;L V Gushchina,&nbsp;V V Filimonov,&nbsp;V P Kutyshenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Protein SHA-D of \"SH3-Bergerac\" chimeric proteins family was constructed by substitution of beta-turn N47-D48 in spectrin SH3-domain by KATANDKTYE amino acid sequence. Structural and dynamics properties of SHA-D in solution were studied by with the help of high-resolution NMR. The extension of SHA-D polypeptide chain in comparison with wild type of protein WT-SH3 (~ 17%) practically doesn't affect almost the total molecule topology. 3D-structure of SHA-D is practically identical to the proteins of \"SH3-Bergerac\" family. However there are some differences in dynamic characteristics in the region of substitution. The G52D substitution in SHA-D protein results in a destabilization of the region insertion where the conditions for conformational exchange appear. Destabilization further affects the entire SHA- D molecule making its structure more labile.</p>","PeriodicalId":9325,"journal":{"name":"Bioorganicheskaia khimiia","volume":"36 4","pages":"505-13"},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29294448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Oxidation of dermatan sulfate with a NaOCl-NaBr-2,2,6,6-tetramethylpiperidine-1-oxyl reagent in the water medium]. [用naocl - nabr -2,2,6,6-四甲基哌啶-1-氧试剂在水介质中氧化硫酸皮聚糖]。
Bioorganicheskaia khimiia Pub Date : 2010-05-01
I Iu Ponedel'kina, E A Khaĭbrakhmanova, V N Odinokov, L M Khalilov, U M Dzhemilev
{"title":"[Oxidation of dermatan sulfate with a NaOCl-NaBr-2,2,6,6-tetramethylpiperidine-1-oxyl reagent in the water medium].","authors":"I Iu Ponedel'kina,&nbsp;E A Khaĭbrakhmanova,&nbsp;V N Odinokov,&nbsp;L M Khalilov,&nbsp;U M Dzhemilev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The oxidation of primary hydroxyl groups in dermatan sulfate with the NaOCl-NaBr-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) reagent in an aqueous alkaline environment was carried out for the first time. Modified dermatan sulfates containing hydrated aldehyde (15-50%) and carboxyl (25-100%) groups were obtained.</p>","PeriodicalId":9325,"journal":{"name":"Bioorganicheskaia khimiia","volume":"36 3","pages":"387-91"},"PeriodicalIF":0.0,"publicationDate":"2010-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29133981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Synthesis, structures, and acute toxicity of gossypol nonsymmetrical aldehyde derivatives]. [棉酚不对称醛衍生物的合成、结构和急性毒性]。
Bioorganicheskaia khimiia Pub Date : 2010-05-01
K Z Tiliabaev, F G Kamaev, N L Vypova, A M Iuldashev, B T Ibragimov, S A Talipov
{"title":"[Synthesis, structures, and acute toxicity of gossypol nonsymmetrical aldehyde derivatives].","authors":"K Z Tiliabaev,&nbsp;F G Kamaev,&nbsp;N L Vypova,&nbsp;A M Iuldashev,&nbsp;B T Ibragimov,&nbsp;S A Talipov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Nonsymmetrical aldehyde derivatives of gossypol, a yellow polyphenolic pigment of cottonseed, were synthesized by reactions with ammonia, aniline, 4-aminoantipyrine, and barbituric acid. Their structures were determined by UV spectrophotometry and IR and (1)H NMR spectroscopy methods. Their acute toxicities in white mice were compared with those of gossypol and the corresponding symmetrical analogues. It was demonstrated that in general, the fewer free aldehyde groups that contained the gossypol derivative, the lower its acute toxicity. Only in the case of a nonsymmetrical gossypol derivative bearing a 4-aminoantipyrine residue did we observe a deviation from the above correlation: its symmetrical counterpart was even more toxic, but still less toxic than gossypol.</p>","PeriodicalId":9325,"journal":{"name":"Bioorganicheskaia khimiia","volume":"36 3","pages":"423-8"},"PeriodicalIF":0.0,"publicationDate":"2010-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29133885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[2'-aldehyde oligonucleotides: synthesis and use for affinity modification of DNA-recognizing proteins]. [2'-醛寡核苷酸:合成及其在dna识别蛋白亲和修饰中的应用]。
Bioorganicheskaia khimiia Pub Date : 2010-05-01
E A Khomiakova, E V Kazanova, E M Zubin, E A Kubareva, N V Molochkov, E M Riazanova, T S Oretskaia
{"title":"[2'-aldehyde oligonucleotides: synthesis and use for affinity modification of DNA-recognizing proteins].","authors":"E A Khomiakova,&nbsp;E V Kazanova,&nbsp;E M Zubin,&nbsp;E A Kubareva,&nbsp;N V Molochkov,&nbsp;E M Riazanova,&nbsp;T S Oretskaia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Oligonucleotides with 1,2-diol grouping were prepared from 2'-O-[2-(2,3-dihydroxypropyl)amino-2-oxo-ethyl]uridine 3'-phosphoramidite. The thermal stability of modified DNA duplexes and their ability to form complexes with the p50 subunit of the NF-kappaB transcription factor and (cytosine-5)-DNA methyltransferase SsoII were studied. The periodate oxidation of the l,2-diol grouping of the oligonucleotides resulted in reactive 2'-aldehyde derivatives. The opportunity of their use for the affinity modification of DNA-recognizing proteins was studied.</p>","PeriodicalId":9325,"journal":{"name":"Bioorganicheskaia khimiia","volume":"36 3","pages":"343-53"},"PeriodicalIF":0.0,"publicationDate":"2010-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29133980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Isolation and determination of activity of IgA1 protease from Neisseria meningitidis]. [脑膜炎奈瑟菌IgA1蛋白酶的分离及活性测定]。
Bioorganicheskaia khimiia Pub Date : 2010-01-01
E Iu Iagudaeva, L S Zhigis, O A Razguliaeva, V S Zueva, E E Mel'nikov, V P Zubov, L V Kozlov, A M Bichucher, O V Kotel'nikova, A P Alliluev, A E Avakov, L D Rumsh
{"title":"[Isolation and determination of activity of IgA1 protease from Neisseria meningitidis].","authors":"E Iu Iagudaeva,&nbsp;L S Zhigis,&nbsp;O A Razguliaeva,&nbsp;V S Zueva,&nbsp;E E Mel'nikov,&nbsp;V P Zubov,&nbsp;L V Kozlov,&nbsp;A M Bichucher,&nbsp;O V Kotel'nikova,&nbsp;A P Alliluev,&nbsp;A E Avakov,&nbsp;L D Rumsh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method of the isolation and purification of IgAl protease from a culture of Neisseria meningitidis serogroup A has been developed. Three inactivated intermediates of the production of the meningococcal vaccine, a culture liquid, as well as a supernatant and sediment obtained by the precipitation of bacterial cells by cetavlon, served as a starting material. The purity of IgA1 protease was determined by SDS-PAGE. An immunoenzyme assay for determining the IgA1 protease activity has been devised. The yield of the enzyme with a specific activity of 0.5 to 4 million units/mg from 103 g of the cetavlon precipitate (40 l of culture liquid) was about 600 mug. It was shown that IgAl protease isolated from serogroup A meningococcus is capable of protecting experimental animals (mice) infected with meningococcus of serogroup B.</p>","PeriodicalId":9325,"journal":{"name":"Bioorganicheskaia khimiia","volume":"36 1","pages":"89-97"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28917438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[STAT1: a many-sided transcription factor]. [STAT1:一种多侧转录因子]。
Bioorganicheskaia khimiia Pub Date : 2010-01-01
I A Kostanian, A V Vonarshenko, V M Lipkin
{"title":"[STAT1: a many-sided transcription factor].","authors":"I A Kostanian,&nbsp;A V Vonarshenko,&nbsp;V M Lipkin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cell homeostasis is regulated by numerous signaling proteins. Their main task is to process extracellular signals and activate the intracellular cascades of reactions that lead to the modulation of gene activity. One of the important signaling systems is the STAT family, which is comprised of seven members. Various STATs operate as effective transcription factors delivering cytokine and growth factor signals to the nucleus. The first found and most studied member of this family is STAT1. This review summarizes modern data on the role of STAT1 in the maintenance of cellular homeostasis, and special attention is paid to this protein in the proliferation and apoptosis of immune system cell processes. It is shown that disturbances in the functionality of this molecule might contribute to oncogenesis.</p>","PeriodicalId":9325,"journal":{"name":"Bioorganicheskaia khimiia","volume":"36 1","pages":"15-28"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28916957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Comparison of polypeptide compositions from individual Agelena orientalis spider venoms]. [东方艾格莉娜蜘蛛毒液中多肽成分的比较]。
Bioorganicheskaia khimiia Pub Date : 2010-01-01
Iu M Shliapnikov, S A Kozlov, A A Fedorov, E V Grishin
{"title":"[Comparison of polypeptide compositions from individual Agelena orientalis spider venoms].","authors":"Iu M Shliapnikov,&nbsp;S A Kozlov,&nbsp;A A Fedorov,&nbsp;E V Grishin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Spider venoms are peculiar combinatory libraries of polypeptide molecules that specifically affect various cell targets. However, the question has remained up to now regarding whether the observed diversity of the polypeptides results from the synthesis of the complete library of these molecules by each individual spider or is due to the peculiarity of each zooid producing a limited set of components. We studied the composition of the mixed venom taken from several dozens of zooids of the Central Asian species of the Agelena orientalis and compared it with the venoms of 20 individual spiders of this species. The venoms were qualitatively and quantitatively analyzed by HPLC, mass spectrometry, and amino acid sequencing. It was shown that the individual venoms contain a lesser number of polypeptide components in comparison with the mixed venom and, in addition, differ from each other by the component composition. The set of components produced by single zooids is relatively narrow, and on the whole is a set identical to that of the mixed venom. The polypeptides with a high content in the venom were structurally characterized and compared with the amino acid sequences deduced from the cDNA library of this species.</p>","PeriodicalId":9325,"journal":{"name":"Bioorganicheskaia khimiia","volume":"36 1","pages":"81-8"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28917437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Molecular chaperones]. (分子伴侣’)。
Bioorganicheskaia khimiia Pub Date : 2010-01-01
E E Mel'nikov, T V Rotanova
{"title":"[Molecular chaperones].","authors":"E E Mel'nikov,&nbsp;T V Rotanova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Chaperones are unique remodeling proteins that participate in a great number of intracellular processes and are involved in the correction of protein structure, the prevention of the aggregation of misfolded proteins, the destruction of protein aggregates, and also the unfolding of native protein targets for their translocation across a membrane. In addition to this, Chaperones assist in the dismantling of active oligomers into inactive unfolded monomers for their subsequent photolytic degradation and the assembly of folded subunits into protein assemblies and specific complexes. Data on the structure and functioning of molecular chaperones from five basic families are summarized in the review.</p>","PeriodicalId":9325,"journal":{"name":"Bioorganicheskaia khimiia","volume":"36 1","pages":"5-14"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28916956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Sulfonium derivatives of thioxanthenone, a new class of photodetritylating agents for microarray oligonucleotide synthesis]. [硫代蒽酮的磺化衍生物，一类用于微阵列寡核苷酸合成的新型光去三烷基化剂]。
Bioorganicheskaia khimiia Pub Date : 2010-01-01
A N Siniakov, A A Riabinin, G A Maksakova, V V Shelkovnikov, V A Loskutov, E V Vasil'ev, N V Shekleina
{"title":"[Sulfonium derivatives of thioxanthenone, a new class of photodetritylating agents for microarray oligonucleotide synthesis].","authors":"A N Siniakov,&nbsp;A A Riabinin,&nbsp;G A Maksakova,&nbsp;V V Shelkovnikov,&nbsp;V A Loskutov,&nbsp;E V Vasil'ev,&nbsp;N V Shekleina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The usability of a new class of photo acids, namely, sulfonium hexaphosphates based on thioxanthenone, for the removal of the dimethoxytrityl protective group in the process of oligonucleotide synthesis has been studied in order to search for new detritylating agents for microarray oligodeoxyribonucleotide synthesis. 2,4-Diethyl-9-oxo-10-(4-heptyloxyphenyl)-9H-thioxanthenium hexafluorophosphate has been successfully used for the solid-phase synthesis of (dT)(10).</p>","PeriodicalId":9325,"journal":{"name":"Bioorganicheskaia khimiia","volume":"36 1","pages":"139-41"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28917399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Synthetic peptide immunorphin as an instrument for studying nonopioid beta-endorphin receptor]. [合成肽免疫啡肽作为研究非阿片样β -内啡肽受体的工具]。
Bioorganicheskaia khimiia Pub Date : 2010-01-01
Iu A Kovalitskaia, E V Navolotskaia
{"title":"[Synthetic peptide immunorphin as an instrument for studying nonopioid beta-endorphin receptor].","authors":"Iu A Kovalitskaia,&nbsp;E V Navolotskaia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Research results of the synthetic decapeptide SLTCLVKGFY (the author's term is immunorphin) corresponding to the 364-373 sequence of G heavy-chain human immunoglobulin are summarized. Special attention is paid to the interaction between immunorphin and a nonopioid (insensitive to the opioid antagonist naloxone) beta-endorphin receptor. Using radioligand analysis, data were found regarding the distribution and functions of a nonopioid beta-endorphin receptor in human and animal bodies and the binding characteristics of immunorphin with a nonopioid receptor.</p>","PeriodicalId":9325,"journal":{"name":"Bioorganicheskaia khimiia","volume":"36 1","pages":"47-55"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28917439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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