[Comparison of polypeptide compositions from individual Agelena orientalis spider venoms].

Bioorganicheskaia khimiia Pub Date : 2010-01-01
Iu M Shliapnikov, S A Kozlov, A A Fedorov, E V Grishin
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Abstract

Spider venoms are peculiar combinatory libraries of polypeptide molecules that specifically affect various cell targets. However, the question has remained up to now regarding whether the observed diversity of the polypeptides results from the synthesis of the complete library of these molecules by each individual spider or is due to the peculiarity of each zooid producing a limited set of components. We studied the composition of the mixed venom taken from several dozens of zooids of the Central Asian species of the Agelena orientalis and compared it with the venoms of 20 individual spiders of this species. The venoms were qualitatively and quantitatively analyzed by HPLC, mass spectrometry, and amino acid sequencing. It was shown that the individual venoms contain a lesser number of polypeptide components in comparison with the mixed venom and, in addition, differ from each other by the component composition. The set of components produced by single zooids is relatively narrow, and on the whole is a set identical to that of the mixed venom. The polypeptides with a high content in the venom were structurally characterized and compared with the amino acid sequences deduced from the cDNA library of this species.

[东方艾格莉娜蜘蛛毒液中多肽成分的比较]。
蜘蛛毒液是一种特殊的多肽分子组合文库,可以特异性地影响各种细胞目标。然而,迄今为止的问题仍然是,观察到的多肽的多样性是由于每只蜘蛛合成了完整的这些分子文库,还是由于每只蜘蛛产生有限成分的特性。我们研究了从中亚种Agelena orientalis的几十种动物中提取的混合毒液的成分,并将其与该种蜘蛛的20个个体的毒液进行了比较。采用高效液相色谱法、质谱法和氨基酸测序对毒液进行定性和定量分析。结果表明,与混合毒液相比,单个毒液含有较少数量的多肽成分,并且在成分组成上彼此不同。单个动物产生的一组成分相对较窄,总体上与混合毒液的一组成分相同。对毒液中含量较高的多肽进行了结构表征,并与从该物种cDNA文库中推导出的氨基酸序列进行了比较。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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