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Relationships between lateral hypothalamic orexin circuits and electroacupuncture-induced mitigation of anxiety in a post-traumatic stress disorder model. 下丘脑外侧食欲素回路与电针诱导的创伤后应激障碍模型焦虑缓解的关系
IF 3.7 3区 医学
Brain Research Bulletin Pub Date : 2025-10-10 DOI: 10.1016/j.brainresbull.2025.111578
Xiaoyi Qu, Jia Sun, Chao Zhang, Jiaqi Lu, Yong Xia, Xueyong Shen, Zouqin Huang, Sheng Liu
{"title":"Relationships between lateral hypothalamic orexin circuits and electroacupuncture-induced mitigation of anxiety in a post-traumatic stress disorder model.","authors":"Xiaoyi Qu, Jia Sun, Chao Zhang, Jiaqi Lu, Yong Xia, Xueyong Shen, Zouqin Huang, Sheng Liu","doi":"10.1016/j.brainresbull.2025.111578","DOIUrl":"10.1016/j.brainresbull.2025.111578","url":null,"abstract":"<p><strong>Background: </strong>Post-traumatic stress disorder (PTSD) is a distressing condition characterized by persistent negative affective states. Electroacupuncture (EA) is a clinically recognized, safe, and efficacious treatment for managing negative emotions associated with PTSD. However, the neural circuits involved in the effects of EA on these emotional disturbances remain unclear.</p><p><strong>Methods: </strong>A modified single prolonged stress (MSPS) procedure was used to develop a mouse model presenting PTSD-like anxiety-related behaviors (ALBs). Adeno-associated viral tracing showed excitatory synaptic transmission from the lateral hypothalamus (LH) to the bed nucleus of the stria terminalis (BNST). By combining anterograde and retrograde tracing, ex vivo electrophysiological analysis, and chemogenetic modulation, the study elucidated the modulatory role of the LH<sup>orexin</sup>-BNST circuit in regulating ALBs under the influence of EA.</p><p><strong>Results: </strong>EA significantly reduced ALBs in MSPS mice, as evaluated by open field tests and elevated plus mazes (all P < 0.05). MSPS mice showed reduced c-Fos-positive neuronal activity in the LH orexin after behavioral testing, which was reversed by EA treatment (P < 0.01). EA upregulated orexin type 2 receptor protein expression in the LH and activated LH-BNST neural projections (all P < 0.05). Orexin-A potentiated spontaneous excitatory postsynaptic currents and action potential firing in BNST glutamatergic neurons. Chemogenetic inhibition of the LH<sup>orexin</sup>-BNST circuit suppressed EA-induced anxiolytic behaviors and reduced neuronal activity in LH orexinergic and BNST glutamatergic populations (all P < 0.05). Similarly, chemogenetic activation alleviated ALBs (P < 0.05) and enhanced neuronal activity (P < 0.01), simulating EA's effects.</p><p><strong>Conclusion: </strong>EA regulates synaptic activity in BNST glutamatergic neurons, identifying the LH<sup>orexin</sup>-BNST glutamatergic circuit as a key mediator of EA-induced anxiolytic effects and a possible therapeutic target for PTSD management.</p>","PeriodicalId":9302,"journal":{"name":"Brain Research Bulletin","volume":" ","pages":"111578"},"PeriodicalIF":3.7,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Resting-state functional magnetic resonance imaging reveals the effects of intravesical electrical stimulation on brain activity and functional connectivity in rats with detrusor underactivity 静息状态功能磁共振成像揭示了膀胱内电刺激对逼尿肌活动不足大鼠脑活动和功能连通性的影响。
IF 3.7 3区 医学
Brain Research Bulletin Pub Date : 2025-10-09 DOI: 10.1016/j.brainresbull.2025.111576
Han Deng , Limin Liao , Xing Li , Yixi Liu , Zitian He , Haoyu Sun
{"title":"Resting-state functional magnetic resonance imaging reveals the effects of intravesical electrical stimulation on brain activity and functional connectivity in rats with detrusor underactivity","authors":"Han Deng ,&nbsp;Limin Liao ,&nbsp;Xing Li ,&nbsp;Yixi Liu ,&nbsp;Zitian He ,&nbsp;Haoyu Sun","doi":"10.1016/j.brainresbull.2025.111576","DOIUrl":"10.1016/j.brainresbull.2025.111576","url":null,"abstract":"<div><h3>Introduction</h3><div>The central mechanisms of intravesical electrical stimulation (IVES) were explored in a rat model of detrusor underactivity (DU) induced by bilateral pelvic nerve crush (bPNC). Resting-state functional magnetic resonance imaging (rs-fMRI) was used to evaluate regional homogeneity (ReHo) and functional connectivity (FC).</div></div><div><h3>Methods</h3><div>Eighteen female Sprague-Dawley rats were randomly assigned to sham, bPNC, or IVES groups. The IVES group received 20-minute daily stimulations for 20 days after bPNC. Following treatment, rs-fMRI, cystometry, and immunofluorescence staining for ΔFosB (neuronal activity marker) and GAP43 (axonal plasticity marker) were performed.</div></div><div><h3>Results</h3><div>Compared with bPNC rats, IVES significantly reduced maximum cystometric capacity and post-void residual volume, and increased detrusor pressure variation during voiding. The bPNC group had higher bladder capacity, increased filling-phase pressure variation, and lower voiding pressure than the sham group. Compared to sham and IVES rats, bPNC rats showed reduced ReHo in the right striatum and periaqueductal gray (PAG). FC between the right striatum and bilateral somatosensory cortex, and between the right PAG and left cerebellum, was weaker in bPNC rats. ΔFosB expression in the right striatum and PAG was higher in bPNC rats than in sham and IVES groups.</div></div><div><h3>Conclusions</h3><div>IVES may improve DU via modulation of neuronal activity in the right striatum and PAG and enhancing FC between the right striatum and somatosensory cortex, and between the right PAG and left cerebellum.</div></div>","PeriodicalId":9302,"journal":{"name":"Brain Research Bulletin","volume":"232 ","pages":"Article 111576"},"PeriodicalIF":3.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neuroimaging Study on Brain Structural Network Topological Properties Affected by Obesity in Childen and Adolescents. 儿童和青少年肥胖影响脑结构网络拓扑特性的神经影像学研究。
IF 3.7 3区 医学
Brain Research Bulletin Pub Date : 2025-10-09 DOI: 10.1016/j.brainresbull.2025.111573
Xin Wang, Qian Li, Ruizhu Wang, Fei Yang, Yanli Xi, Ming Yang
{"title":"Neuroimaging Study on Brain Structural Network Topological Properties Affected by Obesity in Childen and Adolescents.","authors":"Xin Wang, Qian Li, Ruizhu Wang, Fei Yang, Yanli Xi, Ming Yang","doi":"10.1016/j.brainresbull.2025.111573","DOIUrl":"https://doi.org/10.1016/j.brainresbull.2025.111573","url":null,"abstract":"<p><strong>Objective: </strong>To examine alterations in the topological properties of brain structural networks in obese adolescents through graph theory analysis.</p><p><strong>Method: </strong>A total of 86 obese adolescents admitted to the Department of Child Health Care at the Children's Hospital of Nanjing Medical University from January 2023 to April 2024 were prospectively enrolled. This cohort included 60 patients in the simple obesity group (Simple OB), 26 patients in the obese group with metabolic syndrome (MS), and 24 healthy volunteers in the control group (CON). All participants underwent DTI scans of the head, anthropometric assessments, and blood tests. Using graph theory, a whole-brain structural network was constructed, and global and nodal topological properties were calculated. Modular analysis was also conducted, and the differences among the three groups were compared using one-way ANOVA. Post-hoc corrections were applied after ANOVA.</p><p><strong>Results: </strong>(1) There were no significant differences in gender, age or fasting blood glucose (FBG) among the three groups. However, there were significant differences in body height(p < 0.001), body weight (p < 0.001), BMI (p < 0.001), Waist circumference (p = 0.006), DBP (p = 0.005), systolic blood pressure (SBP) (p = 0.004), HDL-C (p = 0.002) among the three groups. Compared with the CON group, there were significant differences in TBF (p < 0.001), TG (p < 0.001), TC (p = 0.029), ALT (p < 0.001), AST (p = 0.020), FINS (p = 0.019) between the OB group and the MS group. (2) The small-world attribute (σ), characteristic path length (Lp) and global efficiency (Eg) did not differ significantly among the three groups (p > 0.05). However, the clustering coefficient (Cp) and local efficiency (Eloc) varied among the three groups, with both Cp and Eloc values being lower in the OB group than in the CON group(p = 0.039 and 0.041 respectively). Node level analysis revealed differences in node properties in the left frontal lobe, right insula, right anterior central gyrus, left anterior cingulate gyrus, left anterior cuneus and bilateral occipital lobes. The modular analysis revealed that the connection strength between the Association and Limbic modules in the OB group was significantly higher than that in the CON group (p = 0.033). (3) The Eloc was negatively correlated with TBF(β = - 0.227, p = 0.036), The modularity was negatively correlated with FINS (β = - 0.293, p = 0.006).</p><p><strong>Conclusion: </strong>Adolescent obesity results in aberrant topological properties of brain's structural network, leading to abnormalities in brain regions associated with self-awareness, cognitive control, emotional regulation and sensorimotor functions in adolescents with obesity. At the level of the brain's structural network, these findings may provide insights into the underlying neural mechanisms of obesity and offer imaging evidence to support clinical interventions.</p>","PeriodicalId":9302,"journal":{"name":"Brain Research Bulletin","volume":" ","pages":"111573"},"PeriodicalIF":3.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Altered functional connectivity of reward circuits in adolescents with addictive nonsuicidal self-injury 青少年成瘾性非自杀性自伤的奖励回路功能连通性改变。
IF 3.7 3区 医学
Brain Research Bulletin Pub Date : 2025-10-08 DOI: 10.1016/j.brainresbull.2025.111574
Kaike Liao , Qianqian Liu , Rui Yu , Xinyue Chen , Xinyan Wu , Shiji Peng , Ying Li , Hangyu Li , Xiongxiong Yang , Nian Liu
{"title":"Altered functional connectivity of reward circuits in adolescents with addictive nonsuicidal self-injury","authors":"Kaike Liao ,&nbsp;Qianqian Liu ,&nbsp;Rui Yu ,&nbsp;Xinyue Chen ,&nbsp;Xinyan Wu ,&nbsp;Shiji Peng ,&nbsp;Ying Li ,&nbsp;Hangyu Li ,&nbsp;Xiongxiong Yang ,&nbsp;Nian Liu","doi":"10.1016/j.brainresbull.2025.111574","DOIUrl":"10.1016/j.brainresbull.2025.111574","url":null,"abstract":"<div><h3>Background</h3><div>Non-suicide self-injury (NSSI) behavioral addiction may be related to impaired midbrain dopamine reward system. However, the underlying neural mechanisms remain unclear. The purpose of this study is to explore the alterations in resting-state functional connectivity (rs-FC) between regions of the reward circuits and the whole brain, and to elucidate the link between these rs-FC alterations and clinical characteristics among NSSI adolescents with addictive features.</div></div><div><h3>Methods</h3><div>A total of 37 unmedicated adolescents with NSSI were recruited, including 24 adolescents with addictive NSSI (aNSSI group), 13 adolescents with non-addictive NSSI (nNSSI group), and 25 healthy controls (HCs). Seed-based FC approach was used to examine the FC alterations between regions of the reward circuit and the whole brain. Subsequently, we employed Pearson’s correlation analyses to evaluate the relationship between FC alterations and self-injury.</div></div><div><h3>Results</h3><div>Relative to the nNSSI group, the aNSSI group showed increased in FC between left hippocampus and right caudate nucleus, as well as a decline in FC between left nucleus accumbens and bilateral medial cingulate gyrus, and between left amygdala and right inferior orbitofrontal gyrus. The FC between left hippocampus and right caudate nucleus was positively correlated with the self-injury frequency in the past month.</div></div><div><h3>Conclusions</h3><div>Our findings suggest that marked FC alterations between the amygdala, nucleus accumbens, and hippocampus and whole brain region among adolescents with addictive NSSI versus adolescents with non-addictive NSSI. Moreover, this alteration may be connected to the frequency of self-injury. These findings may provide new neuroimaging evidence for behavioral addiction of NSSI and for distinguishing the subtypes of NSSI.</div></div>","PeriodicalId":9302,"journal":{"name":"Brain Research Bulletin","volume":"232 ","pages":"Article 111574"},"PeriodicalIF":3.7,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Temporal evolution of brain structural changes associated with anxiety sensitivity in patients with breast cancer: A causal network analysis 乳腺癌患者与焦虑敏感性相关的脑结构变化的时间演化:因果网络分析
IF 3.7 3区 医学
Brain Research Bulletin Pub Date : 2025-10-08 DOI: 10.1016/j.brainresbull.2025.111575
Tian-Ye Lin , Shao-Shuai Sun , Yang Yang , Qing-Yang Li , Nan Sun , Ying-Shi Sun
{"title":"Temporal evolution of brain structural changes associated with anxiety sensitivity in patients with breast cancer: A causal network analysis","authors":"Tian-Ye Lin ,&nbsp;Shao-Shuai Sun ,&nbsp;Yang Yang ,&nbsp;Qing-Yang Li ,&nbsp;Nan Sun ,&nbsp;Ying-Shi Sun","doi":"10.1016/j.brainresbull.2025.111575","DOIUrl":"10.1016/j.brainresbull.2025.111575","url":null,"abstract":"<div><h3>Background</h3><div>Anxiety sensitivity (AS) significantly impacts treatment outcomes and quality of life in breast cancer patients, yet the neural mechanisms underlying AS-related brain structural changes remain poorly understood.</div></div><div><h3>Aims</h3><div>To investigate brain structural alterations associated with AS and their temporal evolution in patients with breast cancer.</div></div><div><h3>Methods</h3><div>Eighty-five patients with breast cancer (42 with high AS and 43 with low AS) and 42 healthy controls (HCs) were recruited. Voxel-based morphometry analysis was conducted to examine gray matter volume (GMV) differences between the high and low AS patient groups and the HCs. Causal structural covariance network (CaSCN) analysis was employed to investigate the inferred temporal evolution of GMV alterations in relation to AS levels and disease duration using cross-sectional data.</div></div><div><h3>Results</h3><div>AS levels showed a marked decline within the first two months post-diagnosis before stabilizing. Both patient groups exhibited GMV reduction compared with HCs, with low AS patients showing more widespread atrophy. The left middle cingulum (MCC.L) GMV negatively correlated with AS scores in high AS patients (r = −0.448, <em>P</em> = 0.0029). CaSCN analysis revealed a directional influence pathway from the MCC.L to the right thalamus (THA.R), with the THA.R showing positive self-feedback. Conjunction analysis identified shared effects of decreasing AS and increasing illness duration mediated by the THA.R, resulting in concordant atrophy in regions such as the left cerebellum (lobule VI) and discordant changes in temporal, parietal, and limbic areas.</div></div><div><h3>Conclusions</h3><div>THA.R plays a central role in mediating AS-related brain structural alterations in breast cancer patients, offering potential therapeutic targets for managing AS.</div></div>","PeriodicalId":9302,"journal":{"name":"Brain Research Bulletin","volume":"232 ","pages":"Article 111575"},"PeriodicalIF":3.7,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hypothalamic subunit volumes in schizophrenia with comorbidity of metabolic syndrome 伴有代谢综合征共病的精神分裂症的下丘脑亚基体积
IF 3.7 3区 医学
Brain Research Bulletin Pub Date : 2025-10-07 DOI: 10.1016/j.brainresbull.2025.111569
Jingyu Zhou , Xueguo Wang , Yufan Zhou , Hongyuan Deng , Sisi Jiang , Huan Huang , Lan Yang , Jiangyan Liao , Hui He , Xianmei Luo , Chao Mu , Mingjun Duan , María Luisa Bringas Vega , Gang Yao , Cheng Luo , Dezhong Yao
{"title":"Hypothalamic subunit volumes in schizophrenia with comorbidity of metabolic syndrome","authors":"Jingyu Zhou ,&nbsp;Xueguo Wang ,&nbsp;Yufan Zhou ,&nbsp;Hongyuan Deng ,&nbsp;Sisi Jiang ,&nbsp;Huan Huang ,&nbsp;Lan Yang ,&nbsp;Jiangyan Liao ,&nbsp;Hui He ,&nbsp;Xianmei Luo ,&nbsp;Chao Mu ,&nbsp;Mingjun Duan ,&nbsp;María Luisa Bringas Vega ,&nbsp;Gang Yao ,&nbsp;Cheng Luo ,&nbsp;Dezhong Yao","doi":"10.1016/j.brainresbull.2025.111569","DOIUrl":"10.1016/j.brainresbull.2025.111569","url":null,"abstract":"<div><div>Schizophrenia and metabolic syndrome (MS) are common conditions that frequently co-occur, yet the neurobiological mechanisms underlying their comorbidity remain unclear. This study aimed to investigate whether hypothalamic structural alterations contribute to this comorbidity. A total of 194 participants were included and categorized into four groups based on diagnoses of schizophrenia and MS: schizophrenia patients with/without metabolic syndrome (SZ-wMS and SZ-nMS), and healthy controls with/without metabolic syndrome. T1-weighted structural magnetic resonance imaging (MRI) was acquired. Clinical assessments included metabolic indicators, cognitive function tests, and the Positive and Negative Syndrome Scale. Based on structural MRI, the hypothalamus was segmented into five subunits in each hemisphere. We examined the interaction effects of schizophrenia and MS on the volumes of hypothalamic subunits and conducted partial correlation and moderation analyses to explore clinical relevance. An interaction effect was found in the volume of the right superior tubular subunit (supTub), with SZ-wMS showing the greatest volume reduction. Reduced right supTub volume was associated with elevated fasting blood glucose level and higher negative symptom scores. The association between right supTub volume and negative symptom scores was moderated by triglycerides level. In the SZ-wMS group, reduced right supTub volume was associated with cognitive function scores. These findings suggest that reduced volume of the right supTub may represent a potential mechanism contributing to the comorbidity of schizophrenia and MS. The observed associations with cognitive dysfunction highlight the right supTub as a possible translational target for clinical interventions aimed at improving cognitive deficits in schizophrenia patients with comorbidity of MS.</div></div>","PeriodicalId":9302,"journal":{"name":"Brain Research Bulletin","volume":"232 ","pages":"Article 111569"},"PeriodicalIF":3.7,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Abnormal intrinsic brain activity patterns in patients with carbon monoxide poisoning associated with neurotransmitter profiles. 一氧化碳中毒患者异常的内在脑活动模式与神经递质谱相关。
IF 3.7 3区 医学
Brain Research Bulletin Pub Date : 2025-10-06 DOI: 10.1016/j.brainresbull.2025.111566
Hongkun Liu, Hongyi Zheng, Gengbiao Zhang, Lan Luo, Jiayan Zhuang, Xuewen Wang, Hui Tan, Bixia Wu, Weijia Li, Yingwen Li, Wenbin Zheng
{"title":"Abnormal intrinsic brain activity patterns in patients with carbon monoxide poisoning associated with neurotransmitter profiles.","authors":"Hongkun Liu, Hongyi Zheng, Gengbiao Zhang, Lan Luo, Jiayan Zhuang, Xuewen Wang, Hui Tan, Bixia Wu, Weijia Li, Yingwen Li, Wenbin Zheng","doi":"10.1016/j.brainresbull.2025.111566","DOIUrl":"https://doi.org/10.1016/j.brainresbull.2025.111566","url":null,"abstract":"<p><p>Currently, no study has comprehensively evaluated local neuronal activity in the brain following carbon monoxide poisoning (COP). Moreover, the corresponding pathological changes in brain disease are not randomly distributed, and the molecular mechanism underlying the abnormal neuronal activity pattern associated with COP remains unknown. In this study, low-frequency fluctuation amplitude (ALFF) was combined with neurotransmitter maps to initially explore potential brain activity patterns related to COP and their potential neurochemical basis. In COP patients, brain activity in regions of the higher order association network was decreased, while brain activity in regions of the lower order sensorimotor network was increased, and brain activity in these regions was correlated with the severity of clinical symptoms. In addition, abnormal intrinsic brain activity patterns in COP patients were spatially correlated with the density of neurotransmitter receptors and transporters for monoaminergic neurotransmission (i.e., norepinephrine, serotonin, and dopamine), and N-methyl-D-aspartate receptors. These results suggest that ALFF can characterize COP-related intrinsic brain activity abnormalities, which may be driven by specific neurotransmitter signals in the brain, and that low-frequency fluctuation in specific brain regions can be used to explain clinical symptoms and neurocognitive manifestations.</p>","PeriodicalId":9302,"journal":{"name":"Brain Research Bulletin","volume":" ","pages":"111566"},"PeriodicalIF":3.7,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ATF3 enhancement of CHAC1 expression: A pathway to neuronal ferroptosis in spinal cord injury ATF3增强CHAC1表达:脊髓损伤中神经元铁下垂的途径。
IF 3.7 3区 医学
Brain Research Bulletin Pub Date : 2025-10-03 DOI: 10.1016/j.brainresbull.2025.111568
Chongwen Wang, Congdi Liu, Jialun Wang, Yongjie Ye, Zhi Zhang
{"title":"ATF3 enhancement of CHAC1 expression: A pathway to neuronal ferroptosis in spinal cord injury","authors":"Chongwen Wang,&nbsp;Congdi Liu,&nbsp;Jialun Wang,&nbsp;Yongjie Ye,&nbsp;Zhi Zhang","doi":"10.1016/j.brainresbull.2025.111568","DOIUrl":"10.1016/j.brainresbull.2025.111568","url":null,"abstract":"<div><h3>Background</h3><div>Spinal cord injury (SCI) is a crippling neurological disorder with few therapies. Recent findings show that ferroptosis, an iron-dependent form of programmed cell death, is critical to SCI. This study looks at how activating transcription factor 3 (ATF3) promotes neuronal ferroptosis via cation transport regulator 1 (CHAC1) in SCI mice.</div></div><div><h3>Methods</h3><div>C57BL/6JNifdc mice were utilized to establish an SCI model through contusion injury. The expression and implications of ATF3 and CHAC1 were explored using immunofluorescence, Terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) staining, and quantitative real-time polymerase chain reaction (qRT-PCR). VSC4.1 motor neuron cells injury was induced by H<sub>2</sub>O<sub>2</sub> and employed to further delineate the pathway <em>in vitro</em>, with treatments including siRNA-mediated ATF3 silencing and CHAC1 overexpression. Cell apoptosis and cloney-forming ability were assessed by flow cytometry and colony formation assay. And specific kits were used to detect ferroptosis indicators such as lipid peroxidation, glutathione (GSH) levels, and iron accumulation. The expressions of nuclear factor-erythroid 2 related factor 2 (NRF2) and glutathione peroxidase 4 (GPX4) were detected by western blot.</div></div><div><h3>Results</h3><div>Ferroptosis of neuron cells occurred in SCI mice. ATF3 was significantly upregulated in SCI mice, correlating with increased CHAC1 expression and enhanced neuronal ferroptosis markers. <em>In vitro</em>, ATF3 knockdown reduced ferroptosis and improved cell survival, while CHAC1 overexpression negated these effects, highlighting a critical interaction in ferroptosis regulation.</div></div><div><h3>Conclusions</h3><div>ATF3 enhances CHAC1 expression to promote neuronal ferroptosis in SCI, suggesting that targeting this pathway could offer a novel therapeutic strategy for SCI recovery.</div></div>","PeriodicalId":9302,"journal":{"name":"Brain Research Bulletin","volume":"232 ","pages":"Article 111568"},"PeriodicalIF":3.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modulation of TLR4-mediated inflammatory pathways and oxidative stress by cerium oxide nanoparticles in traumatic brain injury 氧化铈纳米颗粒在创伤性脑损伤中对tlr4介导的炎症通路和氧化应激的调节
IF 3.7 3区 医学
Brain Research Bulletin Pub Date : 2025-10-03 DOI: 10.1016/j.brainresbull.2025.111567
Mohammad Abbas Bejeshk , Mohammad Amin Rajizadeh , Mohammad Khaksari , Mehrdad khatami , Fatemeh Bagheri , Mohadeseh Chahkandi , Ladan Amirkhosravi , Sedigheh Amiresmaili
{"title":"Modulation of TLR4-mediated inflammatory pathways and oxidative stress by cerium oxide nanoparticles in traumatic brain injury","authors":"Mohammad Abbas Bejeshk ,&nbsp;Mohammad Amin Rajizadeh ,&nbsp;Mohammad Khaksari ,&nbsp;Mehrdad khatami ,&nbsp;Fatemeh Bagheri ,&nbsp;Mohadeseh Chahkandi ,&nbsp;Ladan Amirkhosravi ,&nbsp;Sedigheh Amiresmaili","doi":"10.1016/j.brainresbull.2025.111567","DOIUrl":"10.1016/j.brainresbull.2025.111567","url":null,"abstract":"<div><h3>Objects</h3><div>Traumatic brain injury (TBI) induces secondary neurodegeneration by interdependent inflammatory and oxidative processes. While cerium oxide nanoparticles (CeO<sub>2</sub>) exhibit antioxidant potential, regulation of Toll-like receptor 4 (TLR4)-driven neuroinflammation remains to be disclosed. The current study investigates the neuroprotective function of CeO<sub>2</sub> in rat models of diffuse TBI focusing on the modulation of the TLR4 signaling pathway.</div></div><div><h3>Method</h3><div>Thirty-six male Wistar rats (n = 6/group) were randomly assigned to six groups: Sham, DMSO, TBI, and three TBI groups receiving CeO<sub>2</sub> at 0.1, 0.5, or 1 µg/kg. Neuroinflammation (TLR4, TNF-α, IL-1β), markers of oxidative stress (MDA, NO, SOD, GPx), functional recovery through Veterinary Coma Scale (VCS), and histopathological changes were examined.</div></div><div><h3>Results</h3><div>CeO<sub>2</sub> treatment demonstrated significant TLR4 suppression, corresponding with reduced pro-inflammatory cytokine release. The nanoparticles also concurrently inhibited oxidative damage by enhancing endogenous antioxidants. These molecular effects improved neural function, and treated animals were more responsive in motor and alertness tests. Histological analysis showed a reduction in edema in the CeO<sub>2</sub> treated groups compared to the TBI group.</div></div><div><h3>Conclusion</h3><div>The study establishes that CeO<sub>2</sub> exert neuroprotection through following mechanisms: (1) TLR4-mediated anti-inflammatory action and (2) catalytic ROS scavenging. Notably, we identify TLR4 modulation as a previously unrecognized therapeutic target of CeO<sub>2</sub> in TBI. These findings position CeO<sub>2</sub> as a promising multitarget nanotherapeutic for TBI that can treat neuroinflammation and oxidative stress - two principal drivers of secondary injury simultaneously. This research provides groundbreaking evidence for the development of CeO<sub>2</sub> based neuroprotective strategies and offers potential advantages over current pharmacological approaches under clinical evaluation.</div></div>","PeriodicalId":9302,"journal":{"name":"Brain Research Bulletin","volume":"232 ","pages":"Article 111567"},"PeriodicalIF":3.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibition of Sirtuin 2 enhances autophagy and restores neuronal function in aged hippocampal neurons. 抑制Sirtuin 2增强衰老海马神经元的自噬并恢复神经元功能。
IF 3.7 3区 医学
Brain Research Bulletin Pub Date : 2025-10-01 Epub Date: 2025-08-07 DOI: 10.1016/j.brainresbull.2025.111501
Zhenyuan Zhang, Lan Zhang, Yidan Zhang, Yuan Zhao, Ya Gao, Cong Zhang, Dongxiao Li, Xiangjian Zhang, Guofeng Yang, Jian Zhang
{"title":"Inhibition of Sirtuin 2 enhances autophagy and restores neuronal function in aged hippocampal neurons.","authors":"Zhenyuan Zhang, Lan Zhang, Yidan Zhang, Yuan Zhao, Ya Gao, Cong Zhang, Dongxiao Li, Xiangjian Zhang, Guofeng Yang, Jian Zhang","doi":"10.1016/j.brainresbull.2025.111501","DOIUrl":"10.1016/j.brainresbull.2025.111501","url":null,"abstract":"<p><p>Age-related cognitive decline is linked to impaired autophagy and hippocampal dysfunction. This study investigates the role of Sirtuin 2 (SIRT2) in age-related cognitive decline, focusing on its impact on autophagy and hippocampal function. Quantitative proteomic analysis revealed 67 significantly dysregulated proteins in the hippocampus of naturally aged male mice, including upregulated SIRT2 and impaired autophagy. To explore the role of SIRT2 in brain aging and its association with autophagy, naturally aged male mice received AK7, a SIRT2-specific inhibitor, for four consecutive weeks, followed by behavioral assessment using the Morris water maze. Western blot and immunofluorescence analyses were applied to assess mTOR phosphorylation, LC3B-II turnover, and SQSTM1/p62 degradation, complemented by in vitro validation in a D-galactose-induced HT-22 cellular senescence model. The results indicated that AK7 administration improved cognitive performance in aged mice, while simultaneously reducing mTOR phosphorylation and enhancing autophagy markers. In vitro, SIRT2 genetic knockdown restored the mTOR phosphorylation, LC3B-II/LC3I ratio, and SQSTM1/p62 accumulation, while also reducing senescence markers (including TNF-α, P21,Trp53) in D-galactose-induced HT-22 cells. These effects were abolished by mTOR activation, confirming mTOR as a downstream mediator of SIRT2. Our findings highlight SIRT2 inhibition as a promising therapeutic strategy to counteract age-related cognitive decline through the modulation of mTOR-dependent autophagy in the hippocampus.</p>","PeriodicalId":9302,"journal":{"name":"Brain Research Bulletin","volume":" ","pages":"111501"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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